PSIII-12 The effect of maternal dietary isoflavone supplementation and infection with porcine reproductive and respiratory syndrome virus on the growth performance of offspring

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 291-292
Author(s):  
Xuenan Chen
Keyword(s):  
Control Diet ◽  
Liver Weight ◽  
Prenatal Development ◽  
Cell Number ◽  
Whole Body ◽  
Respiratory Syndrome Virus ◽  
Organ Weights ◽  
Relative Liver Weight ◽  
Body Muscle ◽  
Time Point

Abstract The objective of this experiment was to evaluate the effects of maternal infection with porcine reproductive and respiratory syndrome virus (PRRSV) on piglet prenatal development and pre-weaning growth, and the potential for isoflavone supplementation to alleviate these effects. First parity gilts (n = 24) at gestational day (GD) 65 were allotted to one of three treatments: uninfected and fed a diet devoid of isoflavones (CON), infected with PRRSV and fed the control diet (POS), or infected with PRRSV and fed a diet supplemented with 1,500 ppm soy isoflavones (ISF). Gilts were inoculated intranasally with saline or 2.5×104 TCID50/mL of suspended live PRRSV (NADC20 strain) on GD 70. To determine the effects of PRRSV infection in sows, feed intake (GD 70–114) and rectal temperatures (GD 70–91) were recorded daily, while serum TNF-α concentrations were measured weekly. After farrowing (GD 114 ± 2), two piglets closest to the average litter weight were culled either at birth or weaning (21 d of age) to determine body, muscle, and organ weights of offspring pigs. Hematoxylin and eosin staining was performed on left semitendinosus muscles for determination of muscle cell number and size at each time-point. At birth, whole body, muscle, and organ weights were not different (P > 0.22) between groups except that relative liver weight was increased (P < 0.05) in POS compared with CON piglets. At weaning, ISF reduced (P ≤ 0.05) whole body and muscle weights, but greater (P < 0.05) kidney weight compared with CON, and greater (P < 0.05) relative liver weight compared with CON or POS. However, muscle fiber number and size were not different (P > 0.39) between groups at either time-point. These results suggest maternal PRRSV infection altered offspring organ growth, but dietary ISF did not alleviate the detrimental effects of PRRSV infection in sows or offspring.

scholarly journals Maternal nutrient restriction in guinea pigs as an animal model for studying growth-restricted offspring with postnatal catch-up growth

2018 ◽  
Vol 314 (5) ◽  
pp. R647-R654 ◽  
Author(s):  
Catherine L. Nevin ◽  
Evan Formosa ◽  
Yohei Maki ◽  
Brad Matushewski ◽  
Timothy R. H. Regnault ◽  
...  
Keyword(s):  
Growth Rates ◽  
Guinea Pigs ◽  
Disease Risk ◽  
Control Diet ◽  
Whole Body ◽  
Nutrient Restriction ◽  
Organ Weights ◽  
Organ Specific ◽  
Catch Up ◽  
The Impact

We determined the impact of moderate maternal nutrient restriction (MNR) in guinea pigs with fetal growth restriction (FGR) on offspring body and organ weights, hypothesizing that FGR-MNR animals will show catch-up growth but with organ-specific differences. Guinea pig sows were fed ad libitum (Control) or 70% of the control diet from 4 weeks preconception, switching to 90% at midpregnancy (MNR). Control newborns >95 g [appropriate for gestational age (AGA); n = 37] and MNR newborns <85 g (FGR; n = 37) were monitored until neonatal (~25 days) or adult (~110 days) necropsy. Birth weights and body/organ weights at necropsy were used to calculate absolute and fractional growth rates (FRs). FGR-MNR birth weights were decreased ~32% compared with the AGA-Controls. FGR-MNR neonatal whole body FRs were increased ~36% compared with Controls indicating catch-up growth, with values negatively correlated to birth weights indicating the degree of FGR leads to greater catch-up growth. However, the increase in organ FRs in the FGR-MNR neonates compared with Controls was variable, being similar for the brain and kidneys indicating comparable catch-up growth to that of the whole body and twofold increased for the liver but negligible for the heart indicating markedly increased and absent catch-up growth, respectively. While FGR-MNR body and organ weights were unchanged from the AGA-Controls by adulthood, whole body growth rates were increased. These findings confirm early catch-up growth in FGR-MNR guinea pigs but with organ-specific differences and enhanced growth rates by adulthood, which are likely to have implications for structural alterations and disease risk in later life.

scholarly journals Dietary values of astaxanthin and canthaxanthin in Penaeus monodon in the presence and absence of cholesterol supplementation: effect on growth, nutrient digestibility and tissue carotenoid composition

2011 ◽  
Vol 108 (1) ◽  
pp. 80-91 ◽  
Author(s):  
Jin Niu ◽  
Chun-Hou Li ◽  
Yong-Jian Liu ◽  
Li-Xia Tian ◽  
Xu Chen ◽  
...  
Keyword(s):  
Stress Test ◽  
Control Diet ◽  
Penaeus Monodon ◽  
Nutrient Digestibility ◽  
Whole Body ◽  
Retention Efficiency ◽  
Commercial Diet ◽  
Body Muscle ◽  
Plasma Antioxidant Capacity ◽  
Cholesterol Supplementation

Penaeus monodon (mean initial wet weight 1·19 (se 0·01) g) were fed seven diets in triplicate: a control diet (D1) without carotenoids; three diets formulated to supply 0·1 % astaxanthin alone (D2), 0·2 % astaxanthin alone (D3), and a combination of 0·1 % astaxanthin and 1 % cholesterol (D4); three diets with 0·07 % canthaxanthin alone (D5), 0·13 % canthaxanthin alone (D6), and a combination of 0·07 % canthaxanthin and 1 % cholesterol (D7). Weight gain (WG, %), specific growth rate (SGR, %/d) and survival were chosen as parameters of shrimp growth performance. Total antioxidant status (TAS), superoxide dismutase (SOD), aspartate aminotransferase (AST) and alanine aminotransferase (ALT) were chosen as indices of shrimp plasma antioxidant capacity. Meanwhile, digestibility, retention efficiency and tissue carotenoids were also investigated to determine the additive effect of cholesterol on the efficiency of astaxanthin and canthaxanthin. After 74 d rearing, WG and SGR of shrimp fed D2–D4 and D7 were higher than those of shrimp fed D1 (P < 0·05). Shrimp fed D4 had the highest survival. The apparent digestibility coefficients (ADC) of astaxanthin in D2–D4 were higher than those of canthaxanthin in D5–D7 (P < 0·05). Although ADC of astaxanthin were quite high (>98 %) in D2–D4 and no differences were found among them (P>0·05), the carotenoid retention efficiencies in the whole body, muscle and shell (D2–D3 treatments) were considerably low; however, cholesterol supplementation significantly improved the carotenoid retention efficiencies in the whole body, muscle and shell (D4 treatment). Accordingly, the addition of cholesterol also significantly enhanced the carotenoid contents of tissues. Shrimp fed supplemented carotenoid diets (D2–D7) had higher TAS and lower SOD, ALT and AST than shrimp fed D1 (P < 0·05). A low dissolved oxygen stress test was conducted for 7 d after the rearing trial and shrimp survival was also compared among the treatments. The survival of shrimp fed the diets supplemented with astaxanthin or canthaxanthin was higher than that of shrimp fed D1 during the stress test (P < 0·05). In conclusion, all data suggested that astaxanthin was better than canthaxanthin as the dietary carotenoid source in the commercial diet of P. monodon, and the supplement of cholesterol could positively enhance the efficiency of astaxanthin and canthaxanthin.

scholarly journals 52 Alterations of fecal microbiome characteristics by dietary soy isoflavone ingestion in growing pigs infected with porcine reproductive and respiratory syndrome virus

2020 ◽  
Vol 98 (Supplement_3) ◽  
pp. 30-31
Author(s):  
Brooke N Smith ◽  
Stephen A Fleming ◽  
Mei Wang ◽  
Ryan N Dilger
Keyword(s):  
Control Diet ◽  
Growing Pigs ◽  
Respiratory Syndrome Virus ◽  
Post Inoculation ◽  
Long Term Effects ◽  
Unifrac Distance ◽  
Fecal Microbiome ◽  
Microbiome Composition ◽  
Time Points ◽  
Experimental Treatments

Abstract Porcine reproductive and respiratory syndrome virus (PRRSV) is an economically-important disease and ingestion of soy isoflavones (ISF) may benefit PRRSV-infected pigs due to demonstrated anti-inflammatory and anti-viral properties. The objective of this study was to quantify long-term effects of ISF consumption on fecal microbiome characteristics under disease challenge. In total, 96 weaned barrows were group-housed in a BSL-2 containment facility and allotted to 1 of 3 experimental treatments that were maintained throughout the wean-to-finish study: non-infected pigs receiving an ISF-devoid control diet (NC, n=24), and infected pigs receiving either the control diet (PC, n=36) or that supplemented with total ISF in excess of 1,600 mg/kg (ISF, n=36) (Table 1). Following a 7-day adaptation, pigs were inoculated intranasally with either a sham-control (PBS) or live PRRSV (1×105 TCID50/mL, strain NADC20). Fecal samples were collected from 48 individual pigs at pre-infection (-2 days post-inoculation, DPI), peak-infection (10 DPI), and post-infection (144 DPI) time-points and extracted DNA was used for 16S bacterial rRNA sequencing. Differences in bacterial communities among diet groups were evaluated using UniFrac distance matrices (weighted and unweighted) in QIIME. All other data were analyzed by one-way ANOVA performed on transformed data using R. Across all time-points, only minimal differences were observed due to ISF alone. At 10 DPI, PRRSV infection reduced Prevotella 9 genera abundance from approximately 20% to less than 10%, but the specific function of this variety in pigs is unclear. The most notable finding was decreased relative abundance of Actinobacteria at 144 DPI between non-infected and infected treatments (P &lt; 0.05), which is consistent with various dysbioses observed in other disease models. Our findings indicate that differences present were mainly due to PRRSV infection and not strongly influenced by ISF ingestion, which implies previously observed performance benefits conferred by dietary ISF are not likely due to changes in microbiome composition.

scholarly journals Quantifying the dietary potassium requirement of juvenile hybrid tilapia (Oreochromis niloticus×O. aureus)

2001 ◽  
Vol 85 (2) ◽  
pp. 213-218 ◽  
Author(s):  
Shi-Yen Shiau ◽  
Jia-Fen Hsieh
Keyword(s):  
Weight Gain ◽  
Atpase Activity ◽  
Oreochromis Niloticus ◽  
Polynomial Regression ◽  
Control Diet ◽  
Mean Value ◽  
Whole Body ◽  
Feeding Trial ◽  
Dietary Potassium ◽  
K Concentration

An 8 week feeding trial was conducted to determine the dietary K requirement for juvenile hybrid tilapia (Oreochromis niloticus × O. aureus). Purified diets with eight levels (0, 1, 2, 3, 4, 5, 7, 10 g/kg diet) of supplemental K were fed to tilapia. Each diet was fed to three replicate groups of fish initially weighing a mean value of 0.77 (SE 0.01) g/fish in a closed, recirculating rearing system. Weight gain was higher (P<0.05) in fish fed the diets supplemented with 2, 3 and 4 g K/kg diet than in fish fed diet with 10 g K/kg diet and the unsupplemented control diet. Gill Na+-K+ ATPase activity was highest in fish fed the diets supplemented with 1–3 g K/kg diet, followed by fish fed the diet with 5 g K/kg diet and lowest in fish fed the diet with 10 g K/kg diet. Whole-body K content in fish were generally increased as the dietary K supplementation level increased. Analysis by polynomial regression of weight gain and gill Na+-K+ ATPase activity and by linear regression of whole-body K retention of the fish indicated that the adequate dietary K concentration for tilapia is about 2–3 g/kg diet.

scholarly journals Dietary sodium requirement determined for juvenile hybrid tilapia (Oreochromis niloticus × O. aureus) reared in fresh water and seawater

2004 ◽  
Vol 91 (4) ◽  
pp. 585-590 ◽  
Author(s):  
Shi-Yen Shiau ◽  
Li-Shan Lu
Keyword(s):  
Weight Gain ◽  
Atpase Activity ◽  
Fresh Water ◽  
Oreochromis Niloticus ◽  
Polynomial Regression ◽  
Control Diet ◽  
Dietary Treatment ◽  
Basal Diet ◽  
Dietary Sodium ◽  
Whole Body

Two 8-week feeding trials were conducted to determine the dietary Na requirement for juvenile hybrid tilapia (Oreochromis niloticus × O. aureus) reared in fresh water and seawater. In each experiment, NaCl was added to the basal diet at 0, 0·5, 1, 2, 3, 5, or 7g Na/kg diet (fresh water) and at 0, 0·2, 0·5, 0·8, 1·2, 1·5, 2, or 3g Na/kg diet (seawater). Each diet was fed to three replicate groups of fish, individual fish initially weighing 0·69 (se 0·01) g, in a closed, recirculating rearing system. In fresh water, the tilapia fed the diet supplemented with 2g Na/kg diet had significantly (P<0·05) greater weight gain than the fish fed the diets supplemented with ≥3 and ≤0·5g Na/kg diet. Feed efficiency (FE) in fish generally followed the weight-gain pattern. Gill Na+–K+ ATPase activity was highest in the fish fed the diets supplemented with 1–3g Na/kg diet, followed by the fish fed the diet with 7g Na/kg diet and lowest in the fish fed the unsupplemented control diet. In seawater, the weight gain, FE and gill Na+–K+ ATPase activity in fish were not affected by the dietary treatment. Analysis by polynomial regression of weight gain, by broken-line regression of gill Na+–K+ ATPase activity and by linear regression of whole-body Na retention of the fish reared in fresh water, indicated that the adequate dietary Na concentration for tilapia is about 1·5g/kg diet. The present study also suggests that no dietary Na is required for tilapia reared in seawater.

scholarly journals Growth and zinc homeostasis in the severely Zn-deficient rat

1984 ◽  
Vol 52 (3) ◽  
pp. 545-560 ◽  
Author(s):  
R. Giugliano ◽  
D. J. Millward
Keyword(s):  
Body Weight ◽  
Growth Rates ◽  
Control Diet ◽  
Rate Of Change ◽  
Zinc Homeostasis ◽  
Whole Body ◽  
Zn Deficiency ◽  
Control Groups ◽  
Body Weights ◽  
Cyclic Changes

1. Male weanling rats were fed on diets either adequate (55 mg/kg), or severely deficient (0.4 mg/kg) in zinc, either ad lib. or in restricted amounts in four experiments. Measurements were made of growth rates and Zn contents of muscle and several individual tissues.2. Zn-deficient rats exhibited the expected symptoms of deficiency including growth retardation, cyclic changes in food intake and body-weight.3. Zn deficiency specifically reduced whole body and muscle growth rates as indicated by the fact that (a) growth rates were lower in ad lib.-fed Zn-deficient rats compared with rats pair-fed on the control diet in two experiments, (b) Zn supplementation increased body-weights of Zn-deficient rats given a restricted amount of diet at a level at which they maintained weight if unsupplemented, (c) Zn supplementation maintained body-weights of Zn-deficient rats fed a restricted amount of diet at a level at which they lost weight if unsupplemented (d) since the ratio, muscle mass:body-weight was lower in the Zn-deficient rats than in the pair-fed control groups, the reduction in muscle mass was greater than the reduction in body-weight.4. Zn concentrations were maintained in muscle, spleen and thymus, reduced in comparison to some but not all control groups in liver, kidney, testis and intestine, and markedly reduced in plasma and bone. In plasma, Zn concentrations varied inversely with the rate of change of body-weight during the cyclic changes in body-weight.5. Calculation of the total Zn in the tissues examined showed a marked increase in muscle Zn with a similar loss from bone, indicating that Zn can be redistributed from bone to allow the growth of other tissues.6. The magnitude of the increase in muscle Zn in the severely Zn-deficient rat, together with the magnitude of the total losses of muscle tissue during the catabolic phases of the cycling, indicate that in the Zn-deficient rat Zn may be highly conserved in catabolic states.

scholarly journals Lipid requirement of bay snook (Petenia splendida Günther, 1862) juveniles

2020 ◽  
Vol 48 (4) ◽  
pp. 674-685
Author(s):  
Uriel Rodríguez-Estrada ◽  
Otilio Méndez-Marín ◽  
Alfredo Pérez-Morales ◽  
Rafael Martínez-García ◽  
Emyr Peña-Marín ◽  
...  
Keyword(s):  
Polynomial Regression ◽  
Lipid Level ◽  
Control Diet ◽  
Optimal Growth ◽  
Whole Body ◽  
Average Weight ◽  
Lipid Levels ◽  
Commercial Diet ◽  
Highest Weight ◽  
Lipid Requirement

This research study is focused on the lipid requirements of Petenia splendida (0.65 ± 0.19 g) juveniles. Fish were fed with trout commercial diet (Silver Cup™ - 45% protein and 16% lipids), as control diet - CD (which is usually used to feed this species at commercial scale), and four isoproteic (45% crude protein) diet formulated with different lipid levels (5, 10, 15 and 20%). Diets were administered for 60 days by triplicate per treatment. Our results showed that fish fed with 10, 15 and 20% lipid levels were significantly higher (P < 0.05) in average weight and total length. Same treatments were significantly lower (P < 0.05) in terms of FCR compared to those shown in 5% and CD. However, fish fed 20% lipid level, showed a significant decrease in WG and a significant (P < 0.05) increase in FCR compared to those shown in 15% lipid level. Fish fed with 15% showed the highest weight gain and the lowest FCR compared with the rest of the treatments. Lipid efficiency ratio (LER) showed significant (P < 0.05) differences among experimental groups. Compared to other diets containing lipid levels (5, 10, and 20%) fish fed 15% diet recorded the lowest protein and lipid content in whole body. Additionally, based on second order polynomial regression analysis of WG, it was demonstrated that 16.2% lipid provides an optimal growth for P. splendida.

scholarly journals Hepatoprotective effects of hydroethanolic extracts of Crocus sativus tepals, stigmas and leaves on carbon tetrachloride induced acute liver injury in rats

2021 ◽  
Vol 25 (2) ◽  
pp. 178-188
Author(s):  
Sabir Ouahhoud ◽  
◽  
Ilham Touiss ◽  
Amine Khoulati ◽  
Iliass Lahmass ◽  
...  
Keyword(s):  
Carbon Tetrachloride ◽  
Liver Injury ◽  
Total Bilirubin ◽  
Liver Weight ◽  
Crocus Sativus ◽  
Male Rats ◽  
Control Group ◽  
Distilled Water ◽  
Relative Liver Weight ◽  
Hepatoprotective Effects

Introduction: The present study investigated the hepatoprotective effects of stigmas, tepals and leaves of Crocus sativus on carbon tetrachloride (CCL4) induced liver injury in rats. Methods: Hydroethanolic extracts of Crocus sativus (stigmas, tepals and leaves) were administrated daily for 14 days by oral gavage. In the present study, 30 male rats divided into five groups were treated as 1: normal rats gavaged with distilled water; 2: intoxicated rats gavaged with distilled water and injected with CCL4; 3: rats treated with stigmas extract and injected with CCL4; 4: rats treated with tepal extract and injected with CCL4; 5: rats treated with leaf extract and injected with CCL4. Bodyweight and the relative liver weight were determined. Alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), total cholesterol, triglycerides, bilirubin direct and total, total protein, albumin, urea and creatinine measured in plasma. Malondialdehyde (MDA) was quantified in liver homogenate. Results: The experimental data showed that the stigmas and tepals extracts significantly prevented weight body loss and improved the relative liver weight. They significantly protected against elevation of ALT, AST, direct bilirubin, total bilirubin, LDH, ALP, creatinine and MDA. Also, they enhanced significantly total proteins and albumin compared to the CCL4 control group. Moreover, leaves reduced ALT, AST, total bilirubin, LDH and MDA significantly. Conclusion: In conclusion, these results suggest that tepals, stigmas, and leaves extracts of Crocus sativus have hepatoprotective effects on CCL4 induced liver injury in rats.

scholarly journals Effects of food or nutrient restriction on milk vitamin A transfer and neonatal vitamin A stores in the rat

1990 ◽  
Vol 63 (2) ◽  
pp. 351-362 ◽  
Author(s):  
Ana Maria ◽  
G. Pasatiempo ◽  
A. Catharine Ross
Keyword(s):  
Vitamin A ◽  
Maternal Diet ◽  
Control Diet ◽  
Serum Vitamin ◽  
Liver Weight ◽  
Retinol Binding Protein ◽  
Serum Retinol ◽  
Total Food Intake ◽  
Affect Transfer ◽  
Liver Vitamin

We have investigated the effects of maternal diets low in fat or protein, or restricted in total food intake on vitamin A transfer from the dam to her pups. When animals were fed on diets moderately restricted in fat or protein, minimal differences in milk, serum, and liver vitamin A concentrations were observed compared with animals fed on a control diet. In a second study, dams were fed on diets more severely restricted in protein, or fat, or both, or were fed on a control diet equal to 50% of the intake of control rats but containing an equal amount of vitamin A. The quantity of milk obtained from these more severely restricted dams' nipples or the pups' stomachs was greatly reduced; however, there were no differences in milk vitamin A concentration. Body-weight, liver weight, and total liver vitamin A stores of undernourished pups were just half those measured for control pups, although serum vitamin A and serum retinol-binding protein were nearly normal in concentration. We conclude that (a) moderate restrictions in fat or protein in the maternal diet are insufficient to affect transfer of vitamin A to the suckling pup; (b) further dietary restrictions could cause decreased milk production with little change in milk vitamin A concentration and, hence, (c) the neonates' hepatic retinol accumulation during the suckling period is markedly reduced when maternal diets are severely deficient in fat or protein or of normal composition but restricted in amount.

Regulation of calcium balance in the sturgeon Acipenser naccarii: a role for PTHrP

2007 ◽  
Vol 293 (2) ◽  
pp. R884-R893 ◽  
Author(s):  
Juan Fuentes ◽  
Christophe Haond ◽  
Pedro M. Guerreiro ◽  
Nádia Silva ◽  
Deborah M. Power ◽  
...  
Keyword(s):  
Calcium Transport ◽  
Calcium Uptake ◽  
Dietary Calcium ◽  
Calcium Deficiency ◽  
Cell Number ◽  
The Body ◽  
Whole Body ◽  
Calcium Balance ◽  
Parathyroid Hormone Related Protein ◽  
Juvenile Sturgeon

Calcium regulation in sturgeon is of special interest because they are a representative of the ancient fishes possessing mainly cartilaginous skeletons and a supposedly low calcium demand. The present study aimed to characterize the effect of a chronic absence of dietary calcium and the effect of parathyroid hormone-related protein (PTHrPA) (1-34) ( 7 ) on calcium balance in juvenile sturgeon ( Acipenser naccarii). At rest, sturgeon juveniles are in net positive calcium balance, since whole body calcium uptake is significantly higher than efflux and calcium accumulates in the body. To study the importance of dietary calcium, the sturgeon were kept on a calcium-free diet for 8 wk. This manipulation impaired growth as measured by failure to gain weight or increase in length and indicates that dietary calcium is important for growth in sturgeon. An increased whole body calcium uptake partially compensated dietary calcium deficiency and was associated with increased gill chloride cell number in lamellae and filaments in parallel with increased gill Na+K+-ATPase activity. In addition, a single injection of piscine PTHrP(1-34) significantly increased whole body calcium uptake and decreased whole body calcium efflux. Administration of PTHrP significantly increased circulating plasma calcium 4–24 h postinjection. The increase in net calcium transport and increased plasma levels of calcium is consistent with the actions of a hypercalcemic factor. It would appear that the sturgeon rely on calcium for growth and tightly regulate calcium transport. The action in calcium balance is consistent with PTHrP acting as a hypercalcemic factor in sturgeon.

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