195 Sperm motion characteristics of ram semen liquid-stored in a milk egg yolk extender at four temperatures

Abstract Varying temperatures have been used for liquid storage of ram semen and that of other species. This study evaluated motility characteristics of ram semen stored at 5, 10, 15, and 20°C for up to 96 h. Two ejaculates were collected and pooled from each of 6 rams using an artificial vagina and evaluated for motility and concentration. Samples were extended in ultra-high temperature pasteurized skim milk and egg yolk (10% v/v) containing penicillin and streptomycin, diluted to 250 million sperm/mL, and packaged in 0.5 mL straws. Semen was held at 32°C during processing, and straws placed in 500 mL jars for storage at 5°C (refrigerator), 10 and 15°C (refrigerated water bath) and 20°C (air conditioned room temperature). Cooling rates were 0.04, 0.25, 0.44, and 0.02 °C/min, and final temperatures reached after 672, 83, 36, and 567 min in the four storage environments, respectively, with cooling rates faster in a liquid than air environment. Straws from individual rams were removed at 6, 24, 48, 72 and 96 h of storage and analyzed with a computer-assisted sperm analyzer after warming to 36°C. Data were analyzed for the effect of storage time, temperature, and their interaction on sperm motion characteristics. Sperm motion characteristics were not affected over time during storage at 5 and 10°C. At 15°C motility parameters decreased in a curvilinear (P < 0.05) relationship with time (progressive motility: 52.6 to 29.7%; rapid motility: 36.5 to 16.5%), and at 20°C in linear (P < 0.001) relationship (progressive motility: 51.9 to 13.1%; rapid motility: 36.1 to 6.3%). Circular motility decreased (P < 0.05) with increasing temperatures after 72 and 96 h of storage, while local motility was not affected by storage temperature and time. Results suggest storage at 10°C may be a viable alternative to storage at 5°C as retention of motility was similar.

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Effect of fruit-juice on spermatozoa viability and lipid peroxidation of Red Sokoto bucks during liquid storage

Nigerian Journal of Animal Production ◽

10.51791/njap.v41i1.2650 ◽

2021 ◽

Vol 41 (1) ◽

pp. 16-27

Author(s):

J. O. Daramola ◽

T. A. Sorongbe ◽

O. M. Onagbesan ◽

A. V. Jegede ◽

A. O. Ladokun ◽

...

Keyword(s):

Lipid Peroxidation ◽

Cell Damage ◽

Egg Yolk ◽

Protective Effects ◽

Sperm Viability ◽

Progressive Motility ◽

Liquid Storage ◽

Watermelon Juice ◽

And Control

Antioxidants are linked with sperm viability because of their protective effects against cell damage during preservation. In order to enhance the life span of refrigerated buck semen, this study was carried out to determine the effect of fruit-rich antioxidants on spermatozoa viability and lipid peroxidation (LPO) of buck semen during liquid storage. Pooled semen from five Red Sokoto bucks was diluted with Tris-egg yolk based extender and supplemented each with juices from pawpaw tomato and watermelon at 0, 2.5, 5, 7.5 and 10/ 100 ml respectively. Following dilution, the semen samples were assessed subjectively after in vitro storage at 5°C for 24, 48, 72 and 96 hours as regards sperm motility, abnormalities, and acrosome status using a phase-contrast microscope. The concentration of malondialdehyde (MDA) as indices of lipid peroxidation (LPO) in the stored semen was measured in thiobarbituric acid reactive substances (TBARS) at 24, 48, 72 and 96 hours. The results showed highest progressive motility in watermelon juice at 2.5% (P<0.05) during the first 24 hours of storage while the lowest progressive motility was recorded at various levels of pawpaw juice (P<0.05). After 48 hours of storage, extender supplemented with watermelon and tomato juices had better progressive motility compared to control except 7.5% and 10%% of tomato juice (P<0.05). Irrespective of level of juice in the extender, the percentage of intact acrosome was similar among the various juices and control. The results showed that spermatozoa extended with watermelon juice had the lowest (P<0.05) percentage abnormality compared to other extenders at 24, 48, 72 and 96 hours of storage. Higher (P<0.05) percent spermatozoa abnormality compared to other fruit juices and control was observed at 72 and 96 hours of storage in spermatozoa extended with pawpaw juice. Significant reductions of MDA concentrations were achieved by addition of fruit-rich antioxidants to Tris-egg yolk based extender during the first 72 hours and the reduction was much pronounced in extender supplemented with pawpaw juice compared to control (P<0.05). The findings reveal that fruit-rich antioxidants from watermelon and tomato have protective ability to maintain sperm viability and to reduce concentration MDA of buck semen during liquid storage.

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Sperm quality assessments for endangered razorback suckers Xyrauchen texanus

Reproduction ◽

10.1530/rep-10-0153 ◽

2011 ◽

Vol 141 (1) ◽

pp. 55-65 ◽

Cited By ~ 11

Author(s):

Jill A Jenkins ◽

Bruce E Eilts ◽

Amy M Guitreau ◽

Chester R Figiel ◽

Rassa O Draugelis-Dale ◽

...

Keyword(s):

Sperm Quality ◽

Natural Populations ◽

Reproductive Capacity ◽

Dna Integrity ◽

Computer Assisted ◽

Progressive Motility ◽

Isotonic Buffer ◽

Quality Assessments ◽

Sperm Motion

Flow cytometry (FCM) and computer-assisted sperm motion analysis (CASA) methods were developed and validated for use with endangered razorback suckersXyrauchen texanuscollected (n=64) during the 2006 spawning season. Sperm motility could be activated within osmolality ranges noted during milt collections (here 167–343 mOsm/kg). We hypothesized that sperm quality of milt collected into isoosmotic (302 mOsm/kg) or hyperosmotic (500 mOsm/kg) Hanks' balanced salt solution would not differ. Pre-freeze viabilities were similar between osmolalities (79%±6 (s.e.m.) and 76%±7); however, post-thaw values were greater in hyperosmotic buffer (27%±3 and 12%±2;P=0.0065), as was mitochondrial membrane potential (33%±4 and 13%±2;P=0.0048). Visual estimates of pre-freeze motility correlated with total (r=0.7589; range 23–82%) and progressive motility (r=0.7449) by CASA and were associated with greater viability (r=0.5985;P<0.0001). Count (FCM) was negatively correlated with post-thaw viability (r=−0.83;P=0.0116) and mitochondrial function (r=−0.91;P=0.0016). By FCM-based assessments of DNA integrity, whereby increased fluorochrome binding indicated more fragmentation, higher levels were negatively correlated with count (r=−0.77;P<0.0001) and pre-freeze viabilities (r=−0.66;P=0.0004). Fragmentation was higher in isotonic buffer (P=0.0234). To increase reproductive capacity of natural populations, the strategy and protocols developed can serve as a template for use with other imperiled fish species, biomonitoring, and genome banking.

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Effect of three commercial extenders on sperm motility and fertility in liquid ram semen stored at 15 °C or 5 °C

Acta Veterinaria Hungarica ◽

10.1556/004.2019.043 ◽

2019 ◽

Vol 67 (3) ◽

pp. 430-444

Author(s):

Ander Arando ◽

Juan Vicente Delgado ◽

José Manuel León ◽

Sergio Nogales ◽

Francisco Javier Navas-González ◽

...

Keyword(s):

Sperm Motility ◽

Soybean Lecithin ◽

Egg Yolk ◽

Kinematic Parameters ◽

Progressive Motility ◽

Head Displacement ◽

Liquid Storage ◽

Lateral Head

The effect of different extenders on sperm motility and fertility was evaluated during liquid storage of ram semen at 5 °C and 15 °C. The semen was collected, pooled and diluted in three commercial extenders: Inra 96® (INRA) based on skimmed milk, Biladyl® A fraction (BIL) based on egg yolk, and Ovixcell® (OVIX) based on soybean lecithin. Then, sperm motility was evaluated at 0, 6, 24, 48, 72 and 96 h. In order to evaluate fertility, samples stored at 15 °C were used after dilution in INRA and OVIX. Results showed that progressive motility was significantly higher up to 72 h of storage in sperm samples maintained at 5 °C in comparison with 15 °C, similarly for each tested diluent. When samples were stored at 5 °C in OVIX, kinematic parameters such as velocity (except curvilinear velocity, VCL), trajectory [linearity (LIN), straightness (STR), wobble (WOB)], amplitude of lateral head displacement (ALH) and beat/cross frequency (BCF) were higher than in INRA and BIL. No significant differences in pregnancy rate were detected between INRA (62.6%) and OVIX (58.9%). In conclusion, liquid storage at 5 °C with OVIX extender is an interesting option since non-animal components are used, and this extender offers similar in vitro and in vivo efficacy as other extenders containing animal components.

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57 EFFECT OF CHOLESTANOL OR CHOLESTEROL ON THE MOTILITY OF FROZEN GOAT SPERMATOZOA AFTER A THERMAL RESISTANCE TEST

Reproduction Fertility and Development ◽

10.1071/rdv26n1ab57 ◽

2014 ◽

Vol 26 (1) ◽

pp. 142

Author(s):

B. G. Silva ◽

E. A. Moraes ◽

W. C. G. Matos ◽

C. S. Oliveira ◽

W. D. Ferrari Junior ◽

...

Keyword(s):

Thermal Resistance ◽

Liquid Nitrogen ◽

Semen Analysis ◽

Egg Yolk ◽

Water Bath ◽

Resistance Test ◽

Computer Assisted ◽

Progressive Motility ◽

Working Solution ◽

Analysis System

The objective of the present study was to determine the concentration of cholesterol or cholestanol-loaded-cyclodextrin that needs to be added to goat sperm before cryopreservation to optimize its survival. The cholesterol or cholestanol loaded methyl-β-cyclodextrin was prepared as described by Moraes et al. (2010 Anim. Reprod. Sci. 118, 148–154). A working solution of the cholesterol or cholestanol-loaded cyclodextrin was prepared by adding 50 mg of each one to 1 mL of TALP at 37°C and mixing the solution briefly using a vortex mixer. Ejaculates (n = 24) from 5 bucks were used for this experiment. Sperm from each ejaculate were diluted 1 : 1 (vol : vol) in Tris diluent (200 mM Tris, 65 mM citric acid, and 55 mM glucose) and centrifuged at 800 × g for 10 min. The pellets were resuspended to a concentration of 120 × 106 sperm mL–1 in Tris and subdivided into 7 aliquots of 5 mL each (600 × 106 total sperm). Sperm were treated in 7 treatment groups that received no additive (0 mg; control) or different levels of cholesterol or cholestanol (0.75, 1.5, or 3.0 mg/120 × 106 sperm). All treatments were incubated for 15 min at room temperature and then cooled to 4°C over 2 h. The samples were diluted with Tris-egg yolk diluent containing 2% glycerol. The sperm were packaged into 0.5-cc straws and frozen in static liquid nitrogen vapor for 20 min and then straws were plunged into liquid nitrogen and stored until analysed for motility and thermal resistance test using a computer-assisted semen analysis system (CASA). Two straws from each treatment were thawed in a 37°C water bath for 30 s and extended in Tris. For the thermal resistance test, after thawing, 0.5 mL of semen from each treatment was placed in 1.5-mL tubes in a water bath at 37°C for 3 h. At 0, 60, 120, and 180 min, subsamples were evaluated for sperm total and progressive motility using a computer-assisted sperm motion analyzer. A total of 200 spermatozoa were counted in at least 5 different fields. Data were analysed using ANOVA and treatment means were separated, using the SNK test at 5% probability. Cholesterol (0.75 mg; 46.7%) and cholestanol (1.5 mg; 40.5%) produced an increase in progressive motility compared with other treatments after 1 h of incubation (P < 0.05). However, cholestanol (0.75 mg; 39.5 and 31%) was higher for total and progressive motility after 3 h of sperm incubation compared with the control (27 and 17.8%; P < 0.05), respectively. The addition of 0.75 mg of cholestanol in fresh sperm before cryopreservation improved the motility of freeze-thawed goat sperm compared with cholesterol. Therefore, adding cholestanol to goat sperm membranes improved cell cryosurvival. Supported by Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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37 USE OF THERMO-RESISTANCE TEST (TRT) TO EVALUATE EQUINE COOLED SEMEN STORED AT 5°C

Reproduction Fertility and Development ◽

10.1071/rdv22n1ab37 ◽

2010 ◽

Vol 22 (1) ◽

pp. 176

Author(s):

G. Pugliesi ◽

J. M. Silva Filho ◽

C. A. A. Torres ◽

D. M. Rates ◽

P. G. Ker ◽

...

Keyword(s):

Reproductive Potential ◽

Egg Yolk ◽

Resistance Test ◽

Progressive Motility ◽

Effective Use ◽

Short Time ◽

Bovine Species ◽

Artificial Vagina ◽

Sperm Movement ◽

Made In

Evaluation of seminal characteristics is an important step to predict the reproductive potential of equine semen in natural or AI programs. Thermo-resistance test (TRT) has wide acceptance among tests in the bovine species, mainly because of its high correlation with fertility field. However, the TRT for stallion semen has not been widely studied. The objective of this study was to evaluate the effective use of TRT for equine cooled semen diluted with different extenders. Three stallions of Mangalarga Marchador breed aged between 8 and 14 years were used. Five semen samples per stallion were obtained, collected 3 times a week, with the aid of an artificial vagina (adapted Hannover model) using mares in natural estrus as dummy. The semen was diluted in 2 extenders: skim dried milk-glucose (E1) and glycine-egg yolk (E2), packaged in samples containing 12 mL of diluted semen to reach a final concentration of 30 million viable spermatozoa mL-1 and then stored at 5°C in an Equitainer® for 24 h. The cooled semen was warmed at 37°C in a water-bath. Spermatozoal progressive motility and vigor of semen were evaluated at 0 (TRT0), 30 (TRT30), 60 (TRT60), and 90 (TRT90) min after the start of warming. Treatment differences for sperm parameters were determined using ANOVA. The average values of sperm motility during TRT0, TRT30, TRT60, and TRT90 in E1 and E2 were, respectively, (E1) 37.0, 31.3, 23.7, and 19.7 and (E2) 30.3, 23.7, 18.3, and 15.7. The average values of vigor during TRT0, TRT30, TRT60, and TRT90 in E1 and E2 were, respectively, (E1) 2.4, 2.03, 1.53, and 1.43 and (E2) 1.97, 1.53, 1.33, and 1.17. During the test, the progressive motility obtained with E1 was higher (P < 0.05) than that with E2, and is within the patterns of motility considered acceptable only at 0 and 30 min of TRT. The E2 extender gave the worst result of the test, which was below the standards recommended for cooled semen. The seminal characteristics decreased in a very short time of TRT (30 min). This test is for use in insemination program. Thus, this demonstrates that changes in interpretation of the test need to be made in equine semen evaluation. A marked reduction of progressive motility at 30 min of test can be caused by loss of intracellular components or lesions in sperm movement structures. Possibly, availability of cyclic nucleotides involved in oxidative phosphorylation and motility are insufficient, although the mitochondria have the ability to produce energy. The TTR time of 90 min is long for equine cooled semen, and a duration for TTR of 30 min may be more appropriate in this species. Supported by grants from CNPq and CAPES.

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113 Effect of antioxidants on motility and fertility of liquid-stored bovine sperm

Reproduction Fertility and Development ◽

10.1071/rdv32n2ab113 ◽

2020 ◽

Vol 32 (2) ◽

pp. 183

Author(s):

Y. Honkawa ◽

T. Fujikawa ◽

N. Miura ◽

C. Kubota

Keyword(s):

Embryo Development ◽

Sperm Motility ◽

Sperm Concentration ◽

Frozen Storage ◽

Egg Yolk ◽

P Value ◽

Motile Sperm ◽

Progressive Motility ◽

Bovine Sperm ◽

Liquid Storage

It is difficult to maintain sperm in liquid storage for a long time, compared with permanent frozen storage in liquid nitrogen. Antioxidants have been reported to improve the quality and fertility of liquid-stored semen. In this study, we investigated whether antioxidants can extend the motility and fertility of frozen-thawed sperm in liquid storage. Frozen-thawed semen from one Japanese black bull (one ejaculate) was diluted in Tris-citrate-fructose (TCF) diluent with 10% (v/v) egg yolk to a sperm concentration of 1×107 spermmL−1. The antioxidants β-mercaptoethanol (βMe) and glutathione (GSH) were added independently, at various concentrations (0.1, 0.5, 1, and 5mM) to sperm suspensions, and these preparations were compared with Control (no added antioxidant). Sperm suspensions were packaged in centrifuge tubes and placed at 17°C in air and monitored daily until sperm motility had stopped (up to 14 days). Sperm motility was analysed by the Sperm Motility Analysis System (SMAS; Ditect Co. Ltd), and the percentage of progressively motile sperm (straight-line velocity (VSL) of >25μm s−1; Grade A classified by WHO manual), compared with that recorded on Day 0 (100%), was determined each day. For evaluation of fertilizing ability, after incubation in liquid storage for 0, 3, 5, and 7 days, sperm were used for IVF with invitro-matured oocytes (30 oocytes per treatment, three replicates). Embryo development was recorded as the proportion of embryos that reached blastocyst by 8 days after IVF. Data for motility were analysed using one-way ANOVA with Tukey test, and embryo development using chi-squared test. A P-value<0.05 was considered statistically significant. At 7 days, the percentage of progressively motile sperm was significantly higher for 0.5, 1, and 5mM βMe than for Control (30.8%, 48.1%, and 50.3%, vs. 0%, respectively). Treatments with 1 and 5mM βMe maintained some sperm progressive motility for 14 days (9.5% and 14.5%). Treatment with GSH showed the same trend at 7 days (32.2%, 36.3%, and 13.7% for 0.5, 1, and 5mM, vs. 0% for Control); 1 and 5mM GSH maintained sperm progressive motility over 10 days (24.8% and 4.4%). In both antioxidant treatments, embryo development was achieved with sperm stored for up to 5 days (Day 0 vs. Day 5 for 0.1mM βMe: 17.6% vs. 13.8%; for 1.0mM GSH: 26.0% vs. 6.7%; for Control: 17.6% vs. 0%). In this study, antioxidants extended both motility and fertility of frozen-thawed bovine sperm in liquid storage. This result suggests the possibility of application to AI using liquid-stored bovine semen.

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34 The effect of dilution method of beagle dog semen on the survival rate of cryopreserved spermatozoa after thawing

Reproduction Fertility and Development ◽

10.1071/rdv31n1ab34 ◽

2019 ◽

Vol 31 (1) ◽

pp. 143

Author(s):

S. W. Kim ◽

C.-L. Kim ◽

I. S. Jeon ◽

Y. G. Ko ◽

I.-S. Hwang

Keyword(s):

Drug Testing ◽

Semen Analysis ◽

Egg Yolk ◽

Control Group ◽

Dilution Method ◽

Computer Assisted ◽

Motile Sperm ◽

Optimal Method ◽

Beagle Dog ◽

Progressive Motility

The successful cryopreservation of spermatozoa of the beagle dog for AI is essential for the establishment of the genetic banks of drug detection dogs. The beagle dog is widely used for drug testing and chosen for breeding by breeders. However, the use of cryopreserved beagle semen is limited by the lower number of offspring of dog species. In this study, 3 highly trained beagle dogs were chosen and their semen was cryopreserved for the next generation. The effects of dilution methods of beagle semen were tested using a direct dilution method at RT and a 2-step dilution method at 5°C. As a control group, the effects of a direct dilution method of semen on the percentage of motile sperm and progressive motility were analysed by computer-assisted semen analysis system (SAIS, Korea), and abnormality of spermatozoa was examined by Diff Quik staining. A total of 9 samples from 3 dogs were extended in 4% glycerol containing Tris-egg yolk diluents at approximately 22 to 25°C. The diluted semen was cooled to 5°C within 2h. The packed 0.5-mL straws were placed 5cm above the surface of LN for 10min and then plunged in. A 2-step dilution method was conducted using the same procedures of freezing, but the first dilution was done with glycerol-free diluent. After cooling to 5°C within 2h, the second diluent with 8% glycerol was added to the same volume of diluted semen at 5°C and stabilised for 1h. After thawing for 45s at 37°C, the semen from the 2-step dilution method showed the higher percentage of motile sperm (65.4±6% v. 45.3±8%; P<0.05) and progressive motility (41.6±5.3% v. 32.3±3.7%; P<0.05). However, the abnormalities between groups showed no differences. The results suggest that the optimal method for freezing beagle dog spermatozoa is a 2-step dilution process that consists of the first dilution at RT and the second dilution with glycerol at 5°C into diluted semen.

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Low density lipoprotein on poor quality mithun (Bos frontalis) semen preservation

Indian Journal of Animal Research ◽

10.18805/ijar.v0iof.4568 ◽

2016 ◽

Author(s):

P. Perumal ◽

S. K. Srivastava ◽

K. K. Baruah ◽

J. S. Rajoriya ◽

N. Srivastava

Keyword(s):

Density Lipoprotein ◽

Egg Yolk ◽

Cholesterol Content ◽

Poor Quality ◽

Computer Assisted ◽

Low Density ◽

Liquid Storage ◽

Group I ◽

Intracellular Enzymes ◽

Group Ii

Low density lipoproteins (LDL) extracted from hens egg yolk (EY) has been studied over EY based extender for liquid storage of mithun semen with the objective to explore the use of LDL in place of EY. Physio-morphological attributes (PMAs) and mobility and velocity parameters were measured by computer assisted sperm analyser (CASA). Leakage of intracellular enzymes, activity of total antioxidants and lipid peroxidation following liquid storage (5oC) of mithun semen were studied. Fifty ejaculates were collected through transrectal massage method from matured mithun bulls and based on the mass activity and individual motility; the semen samples were splited into good and poor quality and diluted with the tris citrate glycerol (TCG) extender and were splited into three equal aliquots: Group I: Control, EY; Group II and Group III contained 8 and 10% LDL (w/v), respectively. PMAs, intracellular enzymatic leakage and biochemical profiles were evaluated at 5°C following 10hrs incubation. Result revealed a significant (p less than 0.05) improvement in PMAs, CASA parameters and cholesterol content of spermatozoa as well as reduction in leakage of intracellular enzymes, oxidative stress in Group II than control and other treatment group. It was concluded that addition of 8% LDL holds a clear advantage over EY or 10% LDL in liquid preservation of mithun semen.

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Ram semen preserved at 0°C with soybean lecithin Tris-based extender substituted for egg yolk

Animal Bioscience ◽

10.5713/ajas.20.0118 ◽

2021 ◽

Vol 34 (2) ◽

pp. 192-197

Author(s):

Jian-qing Zhao ◽

Guo-liang Xiao ◽

Wen-liang Zhu ◽

Di Fang ◽

Na Li ◽

...

Keyword(s):

Soybean Lecithin ◽

Egg Yolk ◽

Control Group ◽

Progressive Motility ◽

Significant Difference ◽

Acrosome Integrity ◽

Normal Offspring ◽

And Control ◽

Ram Sperm ◽

Artificial Vagina

Objective: The present study evaluated the preservation of ram semen at 0°C using soybean lecithin with a Tris-fructose extender.Methods: Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×10<sup>8</sup> sperm/mL, followed by cooling to 0°C in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination.Results: The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216 h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05).Conclusion: These results suggest that ram sperm is capable of fertilization after preservation at 0°C with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.

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Effect of semen extenders on sperm motion of in vitro stored muscovy drake spermatozoa

Biotechnology in Animal Husbandry ◽

10.2298/bah1103733g ◽

2011 ◽

Vol 27 (3) ◽

pp. 733-740

Author(s):

V. Gerzilov ◽

P. Rashev ◽

A. Bochukov ◽

P. Bonchev

Keyword(s):

Motion Analysis ◽

Computer Assisted ◽

Sperm Velocity ◽

In Vitro Storage ◽

Motion Parameters ◽

Motion Characteristics ◽

Sperm Motion

A study for the influence of semen extenders IMV-buffer, HIA- 1 and AU on sperm motion characteristics of Muscovy drake spermatozoa was carried out. The semen from each male (n=6) was divided into three equal parts and diluted in ratio 1:3 (semen:extender) with the IMV-buffer, HIA-1 and AU respectively, and then was stored at temperature 0-4C? for 6 hours. Sperm motion parameters - velocity of spermatozoa (rapid, medium, slow and statistic), VCL, VSL, VAP, LIN, STR, WOB were measured using a Sperm Class Analizer (Micropticum, Spain). Computer-assisted sperm motion analysis indicated that Muscovy spermatozoa preserved a rapid and medium sperm velocity after 6 hours in vitro storage in three examination extenders. The total VCL, VSL and VAP of spermatozoa in the semen diluted with AU extender were 110.47?7.44 ?m/s, 29.42?2.02 ?m/s and 57.39?3.73 ?m/s, in the semen diluted with IMV-buffer were 94.93?11.10 ?m/s, 27.57?2.45 ?m/s and 51.35?4.98 ?m/s, and in the semen diluted with HIA-1 were 68.48?12.74 ?m/s, 20.08?4.18 ?m/s and 37.75?7.65 ?m/s, as the differences were significant between AU and HIA-1 - (P<0.05). About LIN, STR, WOB there were no significant differences for the influence of the extenders.

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