37 USE OF THERMO-RESISTANCE TEST (TRT) TO EVALUATE EQUINE COOLED SEMEN STORED AT 5°C

2010 ◽  
Vol 22 (1) ◽  
pp. 176
Author(s):  
G. Pugliesi ◽  
J. M. Silva Filho ◽  
C. A. A. Torres ◽  
D. M. Rates ◽  
P. G. Ker ◽  
...  
Keyword(s):  
Reproductive Potential ◽  
Egg Yolk ◽  
Resistance Test ◽  
Progressive Motility ◽  
Effective Use ◽  
Short Time ◽  
Bovine Species ◽  
Artificial Vagina ◽  
Sperm Movement ◽  
Made In

Evaluation of seminal characteristics is an important step to predict the reproductive potential of equine semen in natural or AI programs. Thermo-resistance test (TRT) has wide acceptance among tests in the bovine species, mainly because of its high correlation with fertility field. However, the TRT for stallion semen has not been widely studied. The objective of this study was to evaluate the effective use of TRT for equine cooled semen diluted with different extenders. Three stallions of Mangalarga Marchador breed aged between 8 and 14 years were used. Five semen samples per stallion were obtained, collected 3 times a week, with the aid of an artificial vagina (adapted Hannover model) using mares in natural estrus as dummy. The semen was diluted in 2 extenders: skim dried milk-glucose (E1) and glycine-egg yolk (E2), packaged in samples containing 12 mL of diluted semen to reach a final concentration of 30 million viable spermatozoa mL-1 and then stored at 5°C in an Equitainer® for 24 h. The cooled semen was warmed at 37°C in a water-bath. Spermatozoal progressive motility and vigor of semen were evaluated at 0 (TRT0), 30 (TRT30), 60 (TRT60), and 90 (TRT90) min after the start of warming. Treatment differences for sperm parameters were determined using ANOVA. The average values of sperm motility during TRT0, TRT30, TRT60, and TRT90 in E1 and E2 were, respectively, (E1) 37.0, 31.3, 23.7, and 19.7 and (E2) 30.3, 23.7, 18.3, and 15.7. The average values of vigor during TRT0, TRT30, TRT60, and TRT90 in E1 and E2 were, respectively, (E1) 2.4, 2.03, 1.53, and 1.43 and (E2) 1.97, 1.53, 1.33, and 1.17. During the test, the progressive motility obtained with E1 was higher (P < 0.05) than that with E2, and is within the patterns of motility considered acceptable only at 0 and 30 min of TRT. The E2 extender gave the worst result of the test, which was below the standards recommended for cooled semen. The seminal characteristics decreased in a very short time of TRT (30 min). This test is for use in insemination program. Thus, this demonstrates that changes in interpretation of the test need to be made in equine semen evaluation. A marked reduction of progressive motility at 30 min of test can be caused by loss of intracellular components or lesions in sperm movement structures. Possibly, availability of cyclic nucleotides involved in oxidative phosphorylation and motility are insufficient, although the mitochondria have the ability to produce energy. The TTR time of 90 min is long for equine cooled semen, and a duration for TTR of 30 min may be more appropriate in this species. Supported by grants from CNPq and CAPES.

57 EFFECT OF CHOLESTANOL OR CHOLESTEROL ON THE MOTILITY OF FROZEN GOAT SPERMATOZOA AFTER A THERMAL RESISTANCE TEST

2014 ◽  
Vol 26 (1) ◽  
pp. 142
Author(s):  
B. G. Silva ◽  
E. A. Moraes ◽  
W. C. G. Matos ◽  
C. S. Oliveira ◽  
W. D. Ferrari Junior ◽  
...  
Keyword(s):  
Thermal Resistance ◽  
Liquid Nitrogen ◽  
Semen Analysis ◽  
Egg Yolk ◽  
Water Bath ◽  
Resistance Test ◽  
Computer Assisted ◽  
Progressive Motility ◽  
Working Solution ◽  
Analysis System

The objective of the present study was to determine the concentration of cholesterol or cholestanol-loaded-cyclodextrin that needs to be added to goat sperm before cryopreservation to optimize its survival. The cholesterol or cholestanol loaded methyl-β-cyclodextrin was prepared as described by Moraes et al. (2010 Anim. Reprod. Sci. 118, 148–154). A working solution of the cholesterol or cholestanol-loaded cyclodextrin was prepared by adding 50 mg of each one to 1 mL of TALP at 37°C and mixing the solution briefly using a vortex mixer. Ejaculates (n = 24) from 5 bucks were used for this experiment. Sperm from each ejaculate were diluted 1 : 1 (vol : vol) in Tris diluent (200 mM Tris, 65 mM citric acid, and 55 mM glucose) and centrifuged at 800 × g for 10 min. The pellets were resuspended to a concentration of 120 × 106 sperm mL–1 in Tris and subdivided into 7 aliquots of 5 mL each (600 × 106 total sperm). Sperm were treated in 7 treatment groups that received no additive (0 mg; control) or different levels of cholesterol or cholestanol (0.75, 1.5, or 3.0 mg/120 × 106 sperm). All treatments were incubated for 15 min at room temperature and then cooled to 4°C over 2 h. The samples were diluted with Tris-egg yolk diluent containing 2% glycerol. The sperm were packaged into 0.5-cc straws and frozen in static liquid nitrogen vapor for 20 min and then straws were plunged into liquid nitrogen and stored until analysed for motility and thermal resistance test using a computer-assisted semen analysis system (CASA). Two straws from each treatment were thawed in a 37°C water bath for 30 s and extended in Tris. For the thermal resistance test, after thawing, 0.5 mL of semen from each treatment was placed in 1.5-mL tubes in a water bath at 37°C for 3 h. At 0, 60, 120, and 180 min, subsamples were evaluated for sperm total and progressive motility using a computer-assisted sperm motion analyzer. A total of 200 spermatozoa were counted in at least 5 different fields. Data were analysed using ANOVA and treatment means were separated, using the SNK test at 5% probability. Cholesterol (0.75 mg; 46.7%) and cholestanol (1.5 mg; 40.5%) produced an increase in progressive motility compared with other treatments after 1 h of incubation (P < 0.05). However, cholestanol (0.75 mg; 39.5 and 31%) was higher for total and progressive motility after 3 h of sperm incubation compared with the control (27 and 17.8%; P < 0.05), respectively. The addition of 0.75 mg of cholestanol in fresh sperm before cryopreservation improved the motility of freeze-thawed goat sperm compared with cholesterol. Therefore, adding cholestanol to goat sperm membranes improved cell cryosurvival. Supported by Fundação de Amparo à Ciência e Tecnologia de Pernambuco (FACEPE) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

scholarly journals Ram semen preserved at 0°C with soybean lecithin Tris-based extender substituted for egg yolk

2021 ◽  
Vol 34 (2) ◽  
pp. 192-197
Author(s):  
Jian-qing Zhao ◽  
Guo-liang Xiao ◽  
Wen-liang Zhu ◽  
Di Fang ◽  
Na Li ◽  
...  
Keyword(s):  
Soybean Lecithin ◽  
Egg Yolk ◽  
Control Group ◽  
Progressive Motility ◽  
Significant Difference ◽  
Acrosome Integrity ◽  
Normal Offspring ◽  
And Control ◽  
Ram Sperm ◽  
Artificial Vagina

Objective: The present study evaluated the preservation of ram semen at 0°C using soybean lecithin with a Tris-fructose extender.Methods: Semen was collected by artificial vagina ejaculation from six rams with proven fertility. High quality ejaculates were diluted by soybean lecithin (0.25%, 0.5%, 0.75%, 1.0%, 1.25%) using Tris-fructose extender and control (Tris-fructose egg yolk extender), respectively. The ejaculates were diluted to a concentration of 5×10<sup>8</sup> sperm/mL, followed by cooling to 0°C in 90 min and maintaining the temperature for 12 days. The diluted semen samples were examined and recorded for sperm progressive motility, acrosome integrity at 0, 24, 72, 144, 216, 288 h, respectively. Two hundred and twenty-three ewes were inseminated for 216 h with optimal soybean lecithin concentrated semen or control via trans-cervical insemination.Results: The results showed that there were no differences in sperm progressive motility at 0, 24, 72, and 144 h (p>0.05). After 216 h, the sperm progressive motility in the control group and 0.5% concentration groups was significantly higher when compared to 0.25% concentration (p<0.05). The 0.5% concentration group demonstrated the highest survival rate and had no difference with the control group (p>0.05). At 216 h, the sperm progressive motility of all groups was still above 50%. The acrosome integrity of all groups was decreased with prolongation of storage time, but there was no difference at each time point (p>0.05). There was no significant difference in the lambing rate and pregnancy rate between the 0.5% concentration group and the control group (p>0.05).Conclusion: These results suggest that ram sperm is capable of fertilization after preservation at 0°C with 0.5% of soybean lecithin in Tris-based extender substituted for egg yolk and produce normal offspring after insemination.

scholarly journals 195 Sperm motion characteristics of ram semen liquid-stored in a milk egg yolk extender at four temperatures

2019 ◽  
Vol 97 (Supplement_1) ◽  
pp. 78-78
Author(s):  
Stephan Wildeus ◽  
Dahlia O’Brien
Keyword(s):  
Storage Temperature ◽  
Skim Milk ◽  
Egg Yolk ◽  
Computer Assisted ◽  
Cooling Rates ◽  
Progressive Motility ◽  
Liquid Storage ◽  
Motion Characteristics ◽  
Sperm Motion ◽  
Artificial Vagina

Abstract Varying temperatures have been used for liquid storage of ram semen and that of other species. This study evaluated motility characteristics of ram semen stored at 5, 10, 15, and 20°C for up to 96 h. Two ejaculates were collected and pooled from each of 6 rams using an artificial vagina and evaluated for motility and concentration. Samples were extended in ultra-high temperature pasteurized skim milk and egg yolk (10% v/v) containing penicillin and streptomycin, diluted to 250 million sperm/mL, and packaged in 0.5 mL straws. Semen was held at 32°C during processing, and straws placed in 500 mL jars for storage at 5°C (refrigerator), 10 and 15°C (refrigerated water bath) and 20°C (air conditioned room temperature). Cooling rates were 0.04, 0.25, 0.44, and 0.02 °C/min, and final temperatures reached after 672, 83, 36, and 567 min in the four storage environments, respectively, with cooling rates faster in a liquid than air environment. Straws from individual rams were removed at 6, 24, 48, 72 and 96 h of storage and analyzed with a computer-assisted sperm analyzer after warming to 36°C. Data were analyzed for the effect of storage time, temperature, and their interaction on sperm motion characteristics. Sperm motion characteristics were not affected over time during storage at 5 and 10°C. At 15°C motility parameters decreased in a curvilinear (P < 0.05) relationship with time (progressive motility: 52.6 to 29.7%; rapid motility: 36.5 to 16.5%), and at 20°C in linear (P < 0.001) relationship (progressive motility: 51.9 to 13.1%; rapid motility: 36.1 to 6.3%). Circular motility decreased (P < 0.05) with increasing temperatures after 72 and 96 h of storage, while local motility was not affected by storage temperature and time. Results suggest storage at 10°C may be a viable alternative to storage at 5°C as retention of motility was similar.

scholarly journals Effect Concentration of Moringa (Moringa oleifera Lam) Leaf Extract in Citrate-Egg Yolk in Maintaining Motility and Viability of Spermatozoa of Kacang Goat

2021 ◽  
Vol 16 (4) ◽  
pp. 340-346
Author(s):  
R. R. Dapawole ◽  
I. P. Sirappa
Keyword(s):  
Leaf Extract ◽  
Moringa Oleifera ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Progressive Motility ◽  
Randomized Design ◽  
Effect Concentration ◽  
Completely Randomized Design ◽  
Artificial Vagina ◽  
Moringa Oleifera Lam

This study aimed to determine the effectiveness and the best concentration of Moringa leaf extract (MLE) in the citrate-egg yolk (C-EY) to maintain the motility and viability of spermatozoa kacang goat. Semen was collected from 3 goats aged two years; by using the artificial vagina method. The semen was evaluated macroscopically and microscopically. The semen that had >70% sperm motility and >250x106/ml sperm concentration was divided into 4 equal tubes, each diluted with100% C-EY (P1), 10% MLE+ 90%C-EY (P2), 20% MLE +80% C-EY (P3), and 30% MLE+70% C-EY (P4). The diluted samples were then stored in a refrigerator (3-5?C) and evaluated for motility and viability every 24 hours. The study was designed using a completely randomized design (CRD) consisting of four treatments and five replications. The results showed that the addition of MLE in C-EY significantly affected goat spermatozoa's progressive motility and viability. The data showed that the spermatozoa kept during four days in a diluent of P2 had higher (P<0.05) motility 44.67±4.80% and viability 74.24±4.46%than the other three diluents of P1(36.00±4.70%; 70.10±3.6%), P3(33.67±0.42%; 66.85±4.99%) and P4 (29.67±3.99%; 63.96±5.44%). This study concluded that adding 10% MLE was the best concentration as source energy in 90% C-EY diluents, which effectively maintained the motility and viability of kacang goat spermatozoa for four days of storage at a temperature of 3-5oC.

86 EFFECTS OF THREE CRYOPRESERVATION SYSTEMS ON LONGEVITY OF STALLION SPERM AFTER THAWING

2008 ◽  
Vol 20 (1) ◽  
pp. 123 ◽  
Author(s):  
D. F. Pasquini ◽  
H. N. Ferreira ◽  
F. O. Papa ◽  
J. A. Dell Aqua Jr ◽  
M. A. Alvarenga
Keyword(s):  
Large Scale ◽  
Egg Yolk ◽  
Final Concentration ◽  
Sperm Cells ◽  
Progressive Motility ◽  
Hamilton System ◽  
Nitrogen Vapor ◽  
Frozen Semen ◽  
Horse Industry ◽  
Artificial Vagina

Use of frozen semen by the horse industry is popular. Several extenders have been used to freeze stallion semen; the most used are EDTA-lactose egg yolk and INRA 82. A new extender named Botu-Crio� (BioTech, Botucatu, Sao Paulo, Brazil) has been used on a large scale in Brazil. No comparisons of these extenders have been published. The present experiment was designed to compare the ability of these three extenders to preserve sperm longevity after incubation. One ejaculate from each of 13 stallions was used. After collection using an artificial vagina, semen was filtered and diluted 1:1 with a milk-base extender (Botu-Semen�), split into 3 parts, and then centrifuged (600g/10 min). After removal of the supernatants, each semen pellet was resuspended with an aliquot of one of the extenders (INRA 82, EDTA-Lactose, or Botu-Crio) to a final concentration of 100 � 106 sperm cells mL–1. Semen was packaged in 0.5-mL French straws, and cooled for stabilization for 20 min at 5�C for Botu-Crio (BC), for 2 h at 5�C for INRA 82 (IR), and without stabilization for EDTA-Lactose (EL) extender. After stabilization, the straws were placed in nitrogen vapor for 15 min and then plunged into liquid nitrogen. Before motility evaluation by CASA using the Hamilton System Analyzer (Hamilton Thorne Biosciences, Beverly, MA, USA), straws were thawed (46�C/20 s) and then incubated at 37�C in a dry block. The patterns of total motility (TM) and progressive motility (PM) were evaluated immediately after thawing (T0), and at 10 (T10), 30 (T30), and 60 (T60) min after incubation at 37�C. Statistical analysis was performed using ANOVA and Tukey's test. Total motility, respectively, for IR, EL, and BC was TM0: 10%, 35%, and 57%; TM10: 25%, 37%, and 68%; TM30: 20%, 21%, and 56%; and TM60: 13%, 10%, and 41%. The respective progressive motility was PM0: 3%, 16%, and 28%; PM10: 11%, 16%, and 32%; PM30: 10%, 8%, and 26%; and PM60: 5%, 2%, and 16%. Motilities were always superior (P < 0.05) at all incubation times with the utilization of Botu-Crio extender. A fertility trial comparing the three extenders is in progress.

Kualitas Semen Cair Kambing Boer Berbahan Pengencer Air Kelapa Muda Varietas Viridis Setelah Simpan Dingin

2019 ◽  
Vol 6 (1) ◽  
pp. 78
Author(s):  
Muhammad Ade Salim ◽  
Muhammad Nur Ihsan ◽  
Nur Isnaini ◽  
Trinil Susilawati
Keyword(s):  
Semen Quality ◽  
Block Design ◽  
Animal Husbandry ◽  
Egg Yolk ◽  
Coconut Water ◽  
Boer Goat ◽  
Randomized Block Design ◽  
Frozen Semen ◽  
Artificial Vagina

ABSTRAKAir kelapa muda varietas viridisdapat dijadikan pengencer aletrnatif semen cair bagi program IB di daerah minim sarana semen beku. Tujuan penelitian ini untuk menguji pengaruh penggunaan air kelapa muda viridissebagai bahan pengencer terhadap kualitas semen cair kambing Boer setelah didinginkan. Dilaksanakanselama 3 bulan di Laboratorium Fakultas Peternakan UBUnit SumberSekar,Malang. Metodenya yaitu eksperimen. Semen dari  3 pejantan Boer umur 3-5 tahun, dikoleksi seminggu sekali dengan VB. Air kelapa mudaviridis umur 5-7 bulan serta tris aminomethane sebagai kontrol. Didesain menggunakan Rancangan Acak Kelompok (RAK) dengan 2 perlakuan yaitu P0 (tris aminomethane + 10% KT) dan  P1 (air kelapa muda viridis + 10% KT) masing-masing diulang 10 kali. Data dianalisis dengan analisis Ragam (Anova) dengan software Genstat 18. Variabelnya yaitu motilitas individu, viabilitas dan abnormalitas. Hasil penelitian yaitu motilitas individu pada P1bertahan sampai 4 hari (40,5± 24,3%), viabilitas terbaik sampai hari ke-5 (42±24,6%), abnormalitas terendah di hari ke-7(1,31± 0,6). Kesimpulannya, Pengencer air kelapa muda viridis dapat mempertahankan kualitas semen cair kambing Boer selama 4 hari untuk motilitas dan 5 hari untuk viabilitas.Kata Kunci:pengencer, air kelapa, varietas viridisABSTRACTYoung viridis coconut water could be used as an alternative to liquid semen diluent for artificial insemination program in the area with limited facility for frozen semen production. This study evaluated the use of young coconut water as a diluent on liquid semen quality of Boer goat after cold storage. This study was carried out for 3 months at Sumber Sekar Laboratory, Faculty of Animal Husbandry, University of Brawijaya, Malang. The semen was collected from 3 Boer bucks aged at 3 to 5 years old. The semen collection was done once a week with the aid of artificial vagina. The diluents used were young Viridis coconut (5 to 7 months old) and tris aminomethane. The method used was an experiment in a randomized block design with 2 treatments and 10 replicates. The treatments used were T0: tris aminomethane + 10% egg yolk (control) and T1:  young Viridis coconut water + 10% egg yolk. Data were analyzed by analysis of variance using Genstat 18 software. The variables measured were sperm individual motility, viability, and abnormality. The results showed that the sperm individual motility in T1 survived up to 4 days (40.5± 24.3%), the best viability at 5 days (42.0±24.6%),  while the lowest abnormality at 7 days (1.31±0.6). It could be concluded that: 1. Tris aminomethane diluent has higher quality with the storage length up to 9 days, 2. Young Viridis coconut water diluent could preserve liquid semen quality of Boer goat up to 4 days for sperm motility and 5 days for sperm viability.Keywords: diluents, coconut water, viridis variety

scholarly journals Synthesis of Metal Organic Frameworks by Ball-Milling

Crystals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Cheng-An Tao ◽  
Jian-Fang Wang
Keyword(s):  
Ball Milling ◽  
Large Scale ◽  
Raw Materials ◽  
Metal Organic Frameworks ◽  
Research Topic ◽  
Mass Ratio ◽  
Time Consumption ◽  
Metal Organic ◽  
Short Time ◽  
Made In

Metal-organic frameworks (MOFs) have been used in adsorption, separation, catalysis, sensing, photo/electro/magnetics, and biomedical fields because of their unique periodic pore structure and excellent properties and have become a hot research topic in recent years. Ball milling is a method of small pollution, short time-consumption, and large-scale synthesis of MOFs. In recent years, many important advances have been made. In this paper, the influencing factors of MOFs synthesized by grinding were reviewed systematically from four aspects: auxiliary additives, metal sources, organic linkers, and reaction specific conditions (such as frequency, reaction time, and mass ratio of ball and raw materials). The prospect for the future development of the synthesis of MOFs by grinding was proposed.

scholarly journals ACTUALITY OF APPLICATION OF BIOGAS INSTALLATIONS IN RUSSIA AND ABROAD

2017 ◽  
Vol 12 (2) ◽  
pp. 71-74
Author(s):  
Булат Зиганшин ◽  
Bulat Ziganshin ◽  
Ильназ Кашапов ◽  
Ilnaz Kashapov ◽  
Ильнур Гайфуллин ◽  
...  
Keyword(s):  
Activated Sludge ◽  
Power Plants ◽  
Biogas Production ◽  
Operating Experience ◽  
Farm Animals ◽  
National Program ◽  
Urban Wastewater ◽  
The World ◽  
Short Time ◽  
Made In

The first scientific developments in the field of biogas technologies were made in Russia more than 70 years ago. Since the 50th years one of the main directions was the anaerobic processing of activated sludge and sediments of urban wastewater. This method attracted attention in connection with the idea of obtaining biogas mainly from the manure of farm animals. Thanks to this in the middle of 50th years a number of pilot plants for biogas production were built in Zaporozhia, Belorussian, Georgian, Moldavian branches of All-Russian Institute of Agriculture Electrification, and also in Ekaterinburg. However, the operating experience of these installations was insignificant - one - two seasons. The problem of obtaining and using biogas is given great attention abroad. In a short time, in many countries around the world a whole industry for the production of biogas has emerged. The leader in the development of biogas industry is China. Since the middle of 1970, the National Program for the production of biogas from livestock wastes has been operating in this country. Currently, there are 10 million farm bioreactors. In addition, 40 000 biogas stations, 24 000 biogas treatment plants operate in China, which provides operation of 190 power plants.

scholarly journals Effect of fruit-juice on spermatozoa viability and lipid peroxidation of Red Sokoto bucks during liquid storage

2021 ◽  
Vol 41 (1) ◽  
pp. 16-27
Author(s):  
J. O. Daramola ◽  
T. A. Sorongbe ◽  
O. M. Onagbesan ◽  
A. V. Jegede ◽  
A. O. Ladokun ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Cell Damage ◽  
Egg Yolk ◽  
Protective Effects ◽  
Sperm Viability ◽  
Progressive Motility ◽  
Liquid Storage ◽  
Watermelon Juice ◽  
And Control

Antioxidants are linked with sperm viability because of their protective effects against cell damage during preservation. In order to enhance the life span of refrigerated buck semen, this study was carried out to determine the effect of fruit-rich antioxidants on spermatozoa viability and lipid peroxidation (LPO) of buck semen during liquid storage. Pooled semen from five Red Sokoto bucks was diluted with Tris-egg yolk based extender and supplemented each with juices from pawpaw tomato and watermelon at 0, 2.5, 5, 7.5 and 10/ 100 ml respectively. Following dilution, the semen samples were assessed subjectively after in vitro storage at 5°C for 24, 48, 72 and 96 hours as regards sperm motility, abnormalities, and acrosome status using a phase-contrast microscope. The concentration of malondialdehyde (MDA) as indices of lipid peroxidation (LPO) in the stored semen was measured in thiobarbituric acid reactive substances (TBARS) at 24, 48, 72 and 96 hours. The results showed highest progressive motility in watermelon juice at 2.5% (P<0.05) during the first 24 hours of storage while the lowest progressive motility was recorded at various levels of pawpaw juice (P<0.05). After 48 hours of storage, extender supplemented with watermelon and tomato juices had better progressive motility compared to control except 7.5% and 10%% of tomato juice (P<0.05). Irrespective of level of juice in the extender, the percentage of intact acrosome was similar among the various juices and control. The results showed that spermatozoa extended with watermelon juice had the lowest (P<0.05) percentage abnormality compared to other extenders at 24, 48, 72 and 96 hours of storage. Higher (P<0.05) percent spermatozoa abnormality compared to other fruit juices and control was observed at 72 and 96 hours of storage in spermatozoa extended with pawpaw juice. Significant reductions of MDA concentrations were achieved by addition of fruit-rich antioxidants to Tris-egg yolk based extender during the first 72 hours and the reduction was much pronounced in extender supplemented with pawpaw juice compared to control (P<0.05). The findings reveal that fruit-rich antioxidants from watermelon and tomato have protective ability to maintain sperm viability and to reduce concentration MDA of buck semen during liquid storage.

scholarly journals Ineffable Cultures or Material Devices: What Valuation Studies can Learn from the Disappearance of Ensured Solidarity in a Health Care Market

2015 ◽  
Vol 3 (1) ◽  
pp. 45-73 ◽  
Author(s):  
Teun Zuiderent-Jerak ◽  
Stans Van Egmond
Keyword(s):  
Health Care ◽  
Decisive Role ◽  
Cultural Value ◽  
Health Care Market ◽  
A Value ◽  
Dutch Health ◽  
Short Time ◽  
Regulated Competition ◽  
Made In

Valuation studies addresses how values are made in valuation practices. A next - or rather previous - question becomes: what then makes valuation practices? Two oppositional replies are starting to dominate how that question can be answered: a more materially oriented focus on devices of valuation and a more sociologically inclined focus on ineffable valuation cultures. The debate between proponents of both approaches may easily turn into the kind of leapfrog debates that have dominated many previous discussions on whether culture or materiality would play a decisive role in driving history. This paper explores a less repetitive reply. It does so by analyzing the puzzling case of the demise of solidarity as a core value within the recent Dutch health care system of regulated competition. While “solidarity among the insured” was both a strong cultural value within the Dutch welfare-based health system, and a value that was built into market devices by health economists, within a fairly short time “fairness” became of lesser importance than “competition”. This makes us call for a more historical, relational, and dynamic understanding of the role of economists, market devices, and of culture in valuation studies.

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