scholarly journals 86 Impact of steeping corn DDGS over 72 hours without or with fiber degrading enzymes and protease alone or in combination on concentration of sugars and organic acids and pH in the liquid medium

2019 ◽  
Vol 97 (Supplement_2) ◽  
pp. 49-50
Author(s):  
Youngji Rho ◽  
Rob Patterson ◽  
Elijah Kiarie
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Liquid Medium ◽  
Ph Measurements ◽  
Degrading Enzymes ◽  
Sterile Plastic ◽  
And Control ◽  
The Impact ◽  
Over Time ◽  
Corn Ddgs

Abstract We investigated the impact of steeping corn DDGS over 72 h with or without fiber degrading enzymes (FDE) and protease (PRO) on concentration of sugar, acetic and lactic acid, and pH. The concentration of crude fat, CP, NDF and ADF in DDGS sample was 8.6, 30.8, 36.1, 12.2% DM, respectively. Treatments were: 1) DDGS steeped without enzymes (Control), 2) DDGS steeped with FDE (FDE), 3) DDGS steeped with protease (PRO), 4) DDGS steeped with combination (FDEPRO). Enzymes were added at 1% of DDGS. Briefly, 50g of DDGS was mixed with 500-mL water with or without enzymes in sterile plastic bottles and steeped for 0, 12, 24, 48 and 72-h at 37℃ with continuous agitation. Samples were aliquoted to individual bottle for each timepoint. At each time point, bottles were pulled out from the incubator for pH measurements and liquid medium supernatant for sugars and acids. Highest arabinose, xylose and glucose was observed at 12 and 24 h, followed by decrease at 48 and 72 h. At 12 h, arabinose was higher (P < 0.05) for FDE and FDEPRO than control. The highest xylose and glucose was seen at 12 h for PRO, FDE and FDEPRO while control was highest at 24 h. Acetic acid and lactic acid increased over time. At 72 h, FEDPRO had highest acetic acid compared to control and PRO, while lactic acid was highest for FDE and FDPRO compared with (P < 0.0001) control and PRO. The pH decreased (P < 0.05) over time for all treatments. At 72 h, FDE had lowest pH followed by FDEPRO, PRO and control (P < .0001). Overall, arabinose, xylose and glucose increased to a certain timepoint and decreased, while lactic and acetic acid increased over time, subsequently leading to lowest pH at 72 h. In conclusion, FDE increased concentration of sugars and acids in steeped DDGS whereas PRO had no effect.

Effect of Indoor Wall Climbing on Self-Efficacy and Self-Perceptions of Children with Special Needs

2009 ◽  
Vol 26 (3) ◽  
pp. 259-273 ◽  
Author(s):  
Erin R. Mazzoni ◽  
P. Lynn Purves ◽  
Julie Southward ◽  
Ryan E. Rhodes ◽  
Viviene A. Temple
Keyword(s):  
Special Needs ◽  
Self Efficacy ◽  
Control Group ◽  
Children With Special Needs ◽  
Control Groups ◽  
Self Perceptions ◽  
Self Worth ◽  
And Control ◽  
The Impact ◽  
Over Time

The impact of a six-week indoor wall climbing on the perceptions of self for children with special needs aged 6–12 years was explored. Participants (n = 46) were randomly assigned to the intervention (girls, n = 4; boys, n = 19) and control groups (girls, n = 5; boys, n = 18). Belayers’ and children’s perceptions of efficacy were measured using specifically designed questionnaires and perceptions of competence and global self-worth were measured using Harter’s (1985) Self-Perception Profile for Children for participants with an adaptive age of 8 years or higher. Children’s self-efficacy and belayers’ ratings of children’s efficacy improved significantly, t(21) = 3.9, p = .001, d = .84 and F(2, 44) = 30.03, p < .001, respectively. The children’s judgments of their athletic and social competence and global self-worth, however, did not change over time or differ from the wait-listed control group (p > .05). These results suggest that it is likely that many experiences that enhance self-efficacy may be needed to improve self-perceptions.

scholarly journals Impact of Milk Fortification on the Microbiological and Physicochemical Properties of Set-Type Skimmed Yoghurt Using Three Commercial Soluble Prebiotics

Foods ◽  
2019 ◽  
Vol 8 (6) ◽  
pp. 181 ◽  
Author(s):  
Rui Li ◽  
Qi Ding ◽  
Xin-Huai Zhao
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Physicochemical Properties ◽  
Acid Content ◽  
Titratable Acidity ◽  
Lactobacillus Delbrueckii ◽  
Chemical Compositions ◽  
Acid Fermentation ◽  
Ph Values ◽  
The Impact

The impact of milk fortification on the microbiological and physicochemical properties of a set-type skimmed yoghurt using three commercial soluble prebiotics (inulin, iso-malto-oligosaccharides, and xylo-oligosaccharides) at either 3 or 5 g/kg was assessed. The three prebiotics had an insignificant impact on yoghurt fermentation because all yoghurt samples had similar titratable acidity and similar pH values after their lactic acid fermentation. Regarding the control yoghurt samples without prebiotics usage, the prebiotics-fortified yoghurt samples showed no difference in their main chemical compositions, hardness, syneresis extent, and apparent viscosity (p > 0.05), but had a slightly higher lactic acid content and a viable quantity of starter strains. All yoghurt samples had the same acetic acid content, while propionic and butyric acids were not produced. Yoghurt storage at 4 °C for 21 day gave these yoghurt samples decreased pH values and a viable quantity of starter strains (especially Lactobacillus delbrueckii subsp. bulgaricus) and unchanged acetic acid; however, it increased lactic acid contents. Overall, prebiotics fortification up to 5 g/kg had a completely insignificant impact on the fermentation and quality attributes of yoghurt samples but could possibly improve the health of consumers due to higher dietary fibers and starter strain populations.

scholarly journals OP0279 THE IMPACT OF A REFERRAL STRATEGY FOR AXIAL SPONDYLOARTHRITIS: 12 MONTHS FOLLOW-UP OF PATIENT REPORTED OUTCOMES

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 174.2-174
Author(s):  
M. J. Abdelkadir ◽  
M. Kuijper ◽  
C. Appels ◽  
A. Spoorenberg ◽  
J. Hazes ◽  
...  
Keyword(s):  
Primary Care ◽  
Usual Care ◽  
Axial Spondyloarthritis ◽  
Control Group ◽  
P Value ◽  
Patient Reported ◽  
Referral Strategy ◽  
And Control ◽  
The Impact ◽  
Over Time

Background:Early recognition of axial spondyloarthritis (axSpA) patients is difficult for general practitioners within the large amount of chronic low back pain (CLBP) patients1. As a result, several referral strategies have been developed to help physicians identify patients at risk for axSpA. Most referral strategies were developed in secondary care patients with no available data on their impact. The only referral strategy that was developed and validated in primary CLBP patients is the Case Finding Axial Spondyloarthritis (CaFaSpA) strategy, but required an impact analysis before implementation in daily clinical practice2-3.Objectives:The purpose of this study was to assess the impact of using the CaFaSpA referral strategy on patient reported outcome outcomes (PROs) in primary care patients with CLBP at risk for axSpA.Methods:A clustered randomized controlled trial was performed in a primary care setting in the Netherlands. (ClinicalTrials.gov Identifier:NCT01944163). Each cluster contained the general practices from a single primary care practice and their included patients. Clusters were randomized to either the intervention (use of CaFaSpA referral strategy) or the control group (usual care). Primary outcome was disability after 12 months. Secondary outcome was quality of life, pain and fatigue after 12 months. A linear mixed-effects model was used to explore the effects over time according to intention to treat analysis.Results:In total 679 patients were included within 93 GP clusters. Sixty-four percent of our study population were female and mean age was 36 (7.5) years. Overall RMDQ reduced over time both in the intervention and control group (figure 1). The difference in decrease was not statically significant between the groups (p-value 0.81).Figure 1.Estimated mean RMDQ scores over time for the overall intervention and usual care group.EQ-5D increased by 0.03 after 12 months within the intervention group and 0.01 in the control group (not significant) (table 1). The decrease in pain and fatigue did not differ significantly between the intervention and control group.Table 1.Mean change in PROs after 12 months in the intervention and control groupPROsInterventionUsual careBaseline12 monthsp-valueBaseline12 monthsp-valueEQ-5D mean (SD)0.69 (0.26)0.72 (0.27)0.140.72 (0.24)0.73 (0.25)0.53VAS-pain mean (SD)5.03 (2.42)4.68 (2.69)0.074.96 (2.42)4.55 (2.69)0.02VAS-fatigue mean (SD)5.19 (2.50)5.01 (0.21)0.355.23 (2.45)4.86 (2.73)0.04Conclusion:Although the functional disability due to pain reduces over time, there was no positive effect by referring based on the CaFaSPA model. Further data on PROMs for the axSpA patients are under investigation.References:[1]Jois RN et al. Rheumatology (Oxford) 2008;47:1364-1366.[2]van Hoeven L et al. PLoS One 2015; 22;10(7):e0131963.[2]Moons KG et al. Heart 2012;98(9):691-8.Disclosure of Interests:None declared

scholarly journals Voltammetric pH Measurements in Unadulterated Foodstuffs, Urine, and Serum with 3D-Printed Graphene/poly(lactic Acid) Electrodes

2020 ◽  
Author(s):  
Fakher M. Rabboh ◽  
Glen O'Neil
Keyword(s):  
Lactic Acid ◽  
Excellent Agreement ◽  
Alkali Metals ◽  
Reaction Rates ◽  
Poly Lactic Acid ◽  
Ph Measurements ◽  
Wide Range ◽  
3D Printed ◽  
Glass Ph Electrode ◽  
The Impact

The pH of a system is a critical descriptor of its chemistry – impacting reaction rates, solubility, chemical speciation, and homeostasis. As a result, pH is one of the most commonly measured parameters in food safety, clinical, and environmental laboratories. Glass pH probes are the gold standard for pH measurements, but suffer drawbacks including frequent recalibration, wet storage of the glass membrane, difficulty in miniaturization, and interferences from alkali metals. In this work, we describe a voltammetric pH sensor that uses a 3D-printed graphene/poly(lactic acid) filament electrode that is pretreated to introduce quinone functional groups to the graphene surface. After thoroughly characterizing the pretreatment parameters using outer-sphere and inner-sphere redox couples, we measured pH by reducing the surface-bound quinones, which undergo a pH-dependent 2e<sup>–</sup>/2H<sup>+</sup> reduction. The position of the redox peak was found to shift –60 ± 2 mV pH<sup>-1</sup> at 25 ºC, which is in excellent agreement with the theoretical value predicted by the Nernst Equation (–59.2 mV pH<sup>-1</sup>). Importantly, the sensors did not require the removal of dissolved oxygen prior to successful pH measurements. We investigated the impact of common interfering species (Pb<sup>2+</sup> and Cu<sup>2+</sup>) and found that there was no impact on the measured pH. We subsequently challenged the sensors to measure the pH of unadulterated complex samples including cola, vinegar, serum, and urine, and obtained excellent agreement compared to a glass pH electrode. In addition to the positive analytical characteristics, the sensors are extremely cheap and easy to fabricate, making them highly accessible to a wide range of researchers. These results pave the way for customizable pH sensors that can be fabricated in (nearly) any geometry for targeted applications using 3D-printing.

scholarly journals Study of effectiveness of lactic acid at varroatosis in the apiaries of Tyumen Region, Russia

2020 ◽  
Vol 10 (5) ◽  
pp. 155-159
Author(s):  
T.F. Domatskaya ◽  
A.N. Domatsky ◽  
A.N. Domatsky
Keyword(s):  
Aqueous Solution ◽  
Lactic Acid ◽  
Negative Impact ◽  
Daily Basis ◽  
Control Group ◽  
Control Groups ◽  
Study Objective ◽  
And Control ◽  
The Impact ◽  
Bee Colonies

The study objective is to study the effectiveness of lactic acid in varroatosis and its impact on the development of bee colonies under conditions of the South of Tyumen region. The acaricidal effectiveness of lactic acid was studied in October 2019 using one method in two experiments in 30 broodless bee colonies kept in two apiaries, where bee colonies were divided into 2 groups (experimental - 10 colonies and control groups of 5 colonies each). For treatment of bee colonies from the experimental group, frames with bees were removed from the hives in turn, which were sprayed with a 15.0% aqueous solution of lactic acid in the volume of 5 ml on each side of the honeycomb. Bee colonies were treated twice with an interval of 5 days. The bees in the control group were not treated. Dead mites were registered on a daily basis in all groups within 5 days after each treatment, for these reasons laminated cardboard sheets were placed on the bottom of the hives, which then were taken out and the number of fallen ectoparasites was recorded. Studies on the impact of lactic acid on the development of bee colonies were conducted in 20 brood bee colonies in the period from May to July 2020. It has been found that the effectiveness of treatment of experimental bee colonies with the drug was 90.0 ± 2.1% (apiary No 1) and 7.8 ± 1.9% (apiary No 2). At the same time, the maximum death of mites in colonies was observed during the first day after treatment. In the control groups, the number of mites decreased by 10.9 ± 0.8% and 11.6 ± 1.0%, respectively. It has been established that the treatment of bees with an aqueous solution of lactic acid in the above concentration, dose and multiplicity did not have a negative impact on the development of bee colonies, their strength and food supply, and no deaths of bees and queens were detected during the observation period.

scholarly journals Towards a Starter Culture for Cocoa Fermentation by the Selection of Acetic Acid Bacteria

Fermentation ◽  
2021 ◽  
Vol 7 (1) ◽  
pp. 42
Author(s):  
Lucie Farrera ◽  
Alexandre Colas de la Noue ◽  
Caroline Strub ◽  
Benjamin Guibert ◽  
Christelle Kouame ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Acid Production ◽  
Acetic Acid Bacteria ◽  
Intraspecific Diversity ◽  
Cocoa Bean ◽  
Acetic Acid Production ◽  
Cocoa Bean Fermentation ◽  
The Impact ◽  
Selection Of

Acetic acid bacteria are involved in many food and beverage fermentation processes. They play an important role in cocoa bean fermentation through their acetic acid production. They initiate the development of some of the flavor precursors that are necessary for the organoleptic quality of cocoa, and for the beans’ color. The development of starter cultures with local strains would enable the preservation of the microbial biodiversity of each country in cocoa-producing areas, and would also control the fermentation. This approach could avoid the standardization of cocoa bean fermentation in the producing countries. One hundred and thirty acetic acid bacteria were isolated from three different cocoa-producing countries, and were identified based on their 16S rRNA gene sequence. The predominate strains were grown in a cocoa pulp simulation medium (CPSM-AAB) in order to compare their physiological traits regarding their specific growth rate, ethanol and lactic acid consumption, acetic acid production, and relative preferences of carbon sources. Finally, the intraspecific diversity of the strains was then assessed through the analysis of their genomic polymorphism by (GTG)5-PCR fingerprinting. Our results showed that Acetobacter pasteurianus was the most recovered species in all of the origins, with 86 isolates out of 130 cultures. A great similarity was observed between the strains according to their physiological characterization and genomic polymorphisms. However, the multi-parametric clustering results in the different groups highlighted some differences in their basic metabolism, such as their efficiency in converting carbon substrates to acetate, and their relative affinity to lactic acid and ethanol. The A. pasteurianus strains showed different behaviors regarding their ability to oxidize ethanol and lactic acid into acetic acid, and in their relative preference for each substrate. The impact of these behaviors on the cocoa quality should be investigated, and should be considered as a criterion for the selection of acetic acid bacteria starters.

The impact of chemotherapy on cognition: A longitudinal study in testicular cancer.

2016 ◽  
Vol 34 (2_suppl) ◽  
pp. 487-487
Author(s):  
Guy C. Toner ◽  
Hayley S. Whitford ◽  
Pawel Kalinowski ◽  
Peter S. Grimison ◽  
Martin R. Stockler ◽  
...  
Keyword(s):  
Cognitive Function ◽  
Testicular Cancer ◽  
Visual Learning ◽  
Control Groups ◽  
Perceived Cognitive Function ◽  
And Control ◽  
Prospective Longitudinal ◽  
The Impact ◽  
Over Time

487 Background: Neuropsychological assessments suggest chemotherapy may lead to cognitive impairment but causal links remain unclear. Prospective longitudinal studies are needed to address methodological limitations, with different chemotherapy regimens, appropriate controls and adjusting confounders. Methods: This 16 centre, prospective observational study accrued 145 patients with testicular cancer treated with surgery, or surgery + chemotherapy, as determined by disease staging. Cognition was assessed by CogHealth, a 10-minute online playing-card format tool designed to overcome language limitations, assessment burden, and practice/ceiling effects. Quality of life, fatigue, anxiety, depression and self-perceived cognitive function were also assessed. Comparisons were made between baseline (≤6 months from orchidectomy/pre-chemotherapy) and follow-Up (12-18 months), for 3 groups with sufficient data; cisplatin-based chemotherapy (BEP/EP, n=41), carboplatin (n=20), and controls (n=41). Results: With age as a covariate, linear mixed models were used to compare the 3 groups over time. There were no significant differences between groups over time for cognitive tasks assessing psychomotor function, complex decision making, visual learning, working memory, and visual attention; self-perceived cognitive function matched actual performance. At baseline, for fatigue (p = 0.008), BEP/EP scored significantly worse than carboplatin (d = -0.75) and control groups (d = -0.80). For anxiety (p = 0.005), BEP/EP scored worse than controls (d = -0.91). For all groups anxiety (p = 0.03), emotional (p = 0.001) and functional wellbeing (p = 0.002), improved over time. BEP/EP with poorest scores at baseline, improved the most by follow-up (d = 0.63, -1.24, -1.17, respectively). Only physical wellbeing showed significant effects (p ≤ 0.03) for group, time, and a differing pattern of change across groups; the BEP/EP group scoring worse at baseline compared to carboplatin (d = -0.94) and control groups (d = -1.16), improving by follow-Up (d = -1.21). Conclusions: For patients with testicular cancer there were no statistically significant differences between groups for cognition scores or self-reported cognitive function. Clinical trial information: 12609000545268.

Quantifying Effect of Lactic, Acetic, and Propionic Acids on Growth of Molds Isolated from Spoiled Bakery Products

2015 ◽  
Vol 78 (9) ◽  
pp. 1689-1698 ◽  
Author(s):  
STÉPHANE DAGNAS ◽  
EMILIE GAUVRY ◽  
BERNARD ONNO ◽  
JEANNE-MARIE MEMBRÉ
Keyword(s):  
Acetic Acid ◽  
Lactic Acid ◽  
Propionic Acid ◽  
Radial Growth ◽  
Polynomial Regression ◽  
Inhibitory Effect ◽  
Malt Extract ◽  
Gamma Model ◽  
Acid Effect ◽  
The Impact

The combined effect of undissociated lactic acid (0 to 180 mmol/liter), acetic acid (0 to 60 mmol/liter), and propionic acid (0 to 12 mmol/liter) on growth of the molds Aspergillus niger, Penicillium corylophilum, and Eurotium repens was quantified at pH 3.8 and 25°C on malt extract agar acid medium. The impact of these acids on lag time for growth (λ) was quantified through a gamma model based on the MIC. The impact of these acids on radial growth rate (μ) was analyzed statistically through polynomial regression. Concerning λ, propionic acid exhibited a stronger inhibitory effect (MIC of 8 to 20 mmol/liter depending on the mold species) than did acetic acid (MIC of 23 to 72 mmol/liter). The lactic acid effect was null on E. repens and inhibitory on A. niger and P. corylophilum. These results were validated using independent sets of data for the three acids at pH 3.8 but for only acetic and propionic acids at pH 4.5. Concerning μ, the effect of acetic and propionic acids was slightly inhibitory for A. niger and P. corylophilum but was not significant for E. repens. In contrast, lactic acid promoted radial growth of all three molds. The gamma terms developed here for these acids will be incorporated in a predictive model for temperature, water activity, and acid. More generally, results for μ and λ will be used to identify and evaluate solutions for controlling bakery product spoilage.

scholarly journals Pre-treatment dips to enhance the removal of apple leafcurling midge from apples using high pressure washing

2017 ◽  
Vol 70 ◽  
pp. 315
Author(s):  
N.E.M. Page-Weir ◽  
L.E. Jamieson ◽  
A.J. Hawthorne ◽  
S.P. Redpath ◽  
A. Chhagen ◽  
...  
Keyword(s):  
High Pressure ◽  
Acetic Acid ◽  
Lactic Acid ◽  
Citric Acid ◽  
Hot Water ◽  
Hot Water Treatment ◽  
Pre Treatment ◽  
The Impact ◽  
Uv C ◽  
Improve Access

The presence of apple leafcurling midge (ALCM) on apples is of concern for many of New Zealand’s export apple markets. High pressure washing (HPW) systems have been implemented in export packhouses to reduce the risks associated with various pests; however, higher removal rates of ALCM are required to improve access to challenging markets. A range of pre-treatment dips to improve ALCM removal efficacy when applied before HPW were tested, and the impact of these pre-treatment dips on pest viability determined. Removal of ALCM cocoons by HPW alone was 41%, compared with pre- treatments of hot water at 51˚C (66%), Saturate® (58%), Prospect oil® (57%), malic acid (53%), citric acid (48%), lactic acid (46%), acetic acid (39%), HarvestCide® (37%) or sodium bicarbonate (37%). However, only the hot-water treatment significantly enhanced removal compared with HPW alone. In a second trial, removal of ALCM cocoons with HPW was 69% and hot water + UV-C increased removal to 76%, although this difference was not statistically significant. Overall, none of the pre-treatment dips was found to reduce ALCM viability significantly compared with HPW alone.

scholarly journals Voltammetric pH Measurements in Unadulterated Foodstuffs, Urine, and Serum with 3D-Printed Graphene/poly(lactic Acid) Electrodes

2020 ◽  
Author(s):  
Fakher M. Rabboh ◽  
Glen O'Neil
Keyword(s):  
Lactic Acid ◽  
Excellent Agreement ◽  
Alkali Metals ◽  
Reaction Rates ◽  
Poly Lactic Acid ◽  
Ph Measurements ◽  
Wide Range ◽  
3D Printed ◽  
Glass Ph Electrode ◽  
The Impact

The pH of a system is a critical descriptor of its chemistry – impacting reaction rates, solubility, chemical speciation, and homeostasis. As a result, pH is one of the most commonly measured parameters in food safety, clinical, and environmental laboratories. Glass pH probes are the gold standard for pH measurements, but suffer drawbacks including frequent recalibration, wet storage of the glass membrane, difficulty in miniaturization, and interferences from alkali metals. In this work, we describe a voltammetric pH sensor that uses a 3D-printed graphene/poly(lactic acid) filament electrode that is pretreated to introduce quinone functional groups to the graphene surface. After thoroughly characterizing the pretreatment parameters using outer-sphere and inner-sphere redox couples, we measured pH by reducing the surface-bound quinones, which undergo a pH-dependent 2e<sup>–</sup>/2H<sup>+</sup> reduction. The position of the redox peak was found to shift –60 ± 2 mV pH<sup>-1</sup> at 25 ºC, which is in excellent agreement with the theoretical value predicted by the Nernst Equation (–59.2 mV pH<sup>-1</sup>). Importantly, the sensors did not require the removal of dissolved oxygen prior to successful pH measurements. We investigated the impact of common interfering species (Pb<sup>2+</sup> and Cu<sup>2+</sup>) and found that there was no impact on the measured pH. We subsequently challenged the sensors to measure the pH of unadulterated complex samples including cola, vinegar, serum, and urine, and obtained excellent agreement compared to a glass pH electrode. In addition to the positive analytical characteristics, the sensors are extremely cheap and easy to fabricate, making them highly accessible to a wide range of researchers. These results pave the way for customizable pH sensors that can be fabricated in (nearly) any geometry for targeted applications using 3D-printing.

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