Purple acid phosphatase 10c encodes a major acid phosphatase that regulates plant growth under phosphate-deficient conditions in rice

Abstract Whilst constitutive overexpression of particular acid phosphatases (APases) can increase utilization of extracellular organic phosphate, negative effects are frequently observed in these transgenic plants under conditions of inorganic phosphate (Pi) sufficiency. In this study, we identified rice purple acid phosphatase 10c (OsPAP10c) as being a novel and major APase that exhibits activities associated both with the root surface and with secretion. Two constructs were used to generate the OsPAP10c-overexpression plants by driving its coding sequence with either a ubiquitin promoter (UP) or the OsPAP10c-native promoter (NP). Compared with the UP transgenic plants, lower expression levels and APase activities were observed in the NP plants. However, the UP and NP plants both showed a similar ability to degrade extracellular ATP and both promoted root growth. The growth performance and yield of the NP transgenic plants were better than the wild-type and UP plants in both hydroponic and field experiments irrespective of the level of Pi supply. Overexpression of APase by its native promoter therefore provides a potential way to improve crop production that might avoid increased APase activity in untargeted tissues and its inhibition of the growth of transgenic plants.

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Influences of water deficiency on the productivity of young plants at different sites

Acta Agraria Debreceniensis ◽

10.34101/actaagrar/150/1733 ◽

2018 ◽

pp. 371-378

Author(s):

Katalin Sárdi

Keyword(s):

Crop Production ◽

Production Efficiency ◽

Field Experiments ◽

Nutrient Supply ◽

Limiting Factors ◽

Nutrient Concentrations ◽

Growth Stages ◽

Water Deficiency ◽

Negative Effects ◽

Site Characteristics

Water deficiency has become one of the most limiting factors of crop production in Hungary as the tendency in annual amounts of precipitation shows a decreasing tendency; therefore, it has become similar to those of Southern Europe. The most significant decrease in precipitation occurs typically during spring, approximately 20% of the data expressed in the averages of the last century. Studying the relationship between water deficiency as a stress factor and nutrient supply is important in order to improve the production efficiency of crops. Nowadays, this problem receives outstanding attention presented in numerous papers both in Hungary and globally, however, there are several questions yet to be answered. Our pot experiments were carried out under controlled greenhouse conditions in order to establish new data on these relationships. Experimental soils were typical for Western Transdanubia, taken from long-term field experiments representing four different site characteristics of the region. It was concluded from the results that drought periods during the early growth stages (i.e. 4–5 weeks after emergence) of plants may result in significant decreases in both dry matter production, nutrient concentrations, nutrient uptake and shoot:root ratios. Better nutrient supply, especially potassium, plays a significant role in reducing the negative effects of water deficiency.

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The Arabidopsis Purple Acid Phosphatase AtPAP10 Is Predominantly Associated with the Root Surface and Plays an Important Role in Plant Tolerance to Phosphate Limitation

PLANT PHYSIOLOGY ◽

10.1104/pp.111.183723 ◽

2011 ◽

Vol 157 (3) ◽

pp. 1283-1299 ◽

Cited By ~ 84

Author(s):

Liangsheng Wang ◽

Zheng Li ◽

Weiqiang Qian ◽

Wanli Guo ◽

Xiang Gao ◽

...

Keyword(s):

Acid Phosphatase ◽

Root Surface ◽

Purple Acid Phosphatase ◽

Phosphate Limitation ◽

Plant Tolerance

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Influence of NPK fertilization on weed flora in maize field

Agrokémia és Talajtan ◽

10.1556/agrokem.63.2014.1.15 ◽

2014 ◽

Vol 63 (1) ◽

pp. 139-148 ◽

Cited By ~ 1

Author(s):

Éva Lehoczky ◽

M. Kamuti ◽

N. Mazsu ◽

J. Tamás ◽

D. Sáringer-Kenyeres ◽

...

Keyword(s):

Plant Nutrition ◽

Crop Production ◽

N Availability ◽

Weed Species ◽

Negative Effects ◽

Production Factors ◽

Maize Field ◽

Weed Flora ◽

Npk Fertilization

Plant nutrition is one of the most important intensification factors of crop production. The utilization of nutrients, however, may be modified by a number of production factors, including weed presence. Thus, the knowledge of occurring weed species, their abundance, nutrient and water uptake is extremely important to establish an appropriate basis for the evaluation of their risks or negative effects on crops. That is why investigations were carried out in a long-term fertilization experiment on the influence of different nutrient supplies (Ø, PK, NK, NPK) on weed flora in maize field.The weed surveys recorded similar diversity on the experimental area: the species of A. artemisiifolia, S. halepense and D. stramonium were dominant, but C. album and C. hybridum were also common. These species and H. annuus were the most abundant weeds.Based on the totalized and average data of all treatments, density followed the same tendency in the experimental years. It was the highest in the PK treated and untreated plots, and significantly exceeded the values of NK fertilized areas. Presumably the better N availability promoted the development of nitrophilic weeds, while the mortality of other small species increased.Winter wheat and maize forecrops had no visible influence on the diversity and the intensity of weediness. On the contrary, there were consistent differences in the density of certain weed species in accordance to the applied nutrients. A. artemisiifolia was present in the largest number in the untreated control and PK fertilized plots. The density of S. halepense and H. annuus was also significantly higher in the control areas. The number of their individuals was smaller in those plots where N containing fertilizers were used. Contrary to them, the density of D. stramonium, C. album and C. hybridum was the highest in the NPK treatments.

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The questions of methodology of field experiments in agriculture and crop production

Interdepartmental thematic scientific collection "Agriculture" ◽

10.31073/zem.94.38-44 ◽

2018 ◽

Vol 1 (94) ◽

pp. 38-44

Author(s):

А.M. Malienkо ◽

N.E. Borуs ◽

N.G. Buslaeva

Keyword(s):

Labor Productivity ◽

Crop Production ◽

Field Experiments ◽

Weather Conditions ◽

High Accuracy ◽

Experimental Plot ◽

Increase Labor Productivity ◽

Biometric Parameters ◽

Field Crops ◽

High Condition

In the article, the results of research on the methodology for conducting studies with corn culture under various methods of sowing and weather conditions. The aim of the research was to establish and evaluate the reliability and high accuracy of the experiment, with a decrease in the area's acreage and taking one plant per repetition. Based on the results of the analysis of biometric parameters and yields, the possibility of sampling from 5 to 108 plants was established statistically and mathematically to establish the accuracy of the experiment. The established parameters of sites in experiments with maize indicate the possibility of obtaining much more information from a smaller unit of area, that is, to increase labor productivity not only with tilled crops. This is the goal of further scientific research with other field crops taking 1 plant of repetitions, observing the conditions of leveling the experimental plot according to the fertility of the soil and sowing seeds with high condition. The data obtained give grounds for continuing research on the minimum space required and the sample in the experiments.

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Effects of Ridge Tillage and Straw Mulching on Cultivation the Fresh Faba Beans

Agronomy ◽

10.3390/agronomy11061054 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1054

Author(s):

Bo Li ◽

Xinyu Chen ◽

Xiaoxu Shi ◽

Jian Liu ◽

Yafeng Wei ◽

...

Keyword(s):

Rice Straw ◽

Precision Agriculture ◽

Field Experiments ◽

Root Length Density ◽

Root Surface ◽

High Yield ◽

Root Surface Area ◽

Straw Mulching ◽

Faba Beans ◽

Ridge Tillage

Ridge tillage is an effective agronomic practice and a miniature precision agriculture; however, its effects on the growth of faba beans (Vicia faba L.) are poorly understood. This study aimed to determine the effect of ridge tillage and straw mulching on the root growth, nutrient accumulation and yield of faba beans. Field experiments were conducted during 2016 and 2017 cropping seasons and comprised four treatments: ridge tillage without any mulching (RT), flat tillage without any mulch (FT), flat tillage with rice straw mulched on the ridge tillage (FTRSM) and ridge tillage with rice straw mulched on the ridge tillage (RTRSM). The RT and RTRSM increased soil temperature and decreased soil humidity and improved soil total nitrogen, total phosphorus, available potassium and organic matter. RT and RTRSM increased the root length density, root surface area, root diameter and root activity of faba beans at flowering and harvest periods. The RT and RTRSM also increased the nitrogen, phosphorus, potassium absorption and the yield of faba beans. These results indicated that ridge tillage and straw mulching affect faba bean growth by improving soil moisture conditions and providing good air permeability and effective soil nutrition supply. This study provides a theoretical basis for the high yield cultivation improvement of faba beans.

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Opportunities and limits of controlled-environment plant phenotyping for climate response traits

Theoretical and Applied Genetics ◽

10.1007/s00122-021-03892-1 ◽

2021 ◽

Author(s):

Anna Langstroff ◽

Marc C. Heuermann ◽

Andreas Stahl ◽

Astrid Junker

Keyword(s):

Crop Production ◽

Field Trials ◽

Plant Phenotyping ◽

Controlled Environment ◽

Climate Response ◽

Negative Effects ◽

Phenotypic Data ◽

Non Invasive ◽

Controlled Environments ◽

Response Traits

AbstractRising temperatures and changing precipitation patterns will affect agricultural production substantially, exposing crops to extended and more intense periods of stress. Therefore, breeding of varieties adapted to the constantly changing conditions is pivotal to enable a quantitatively and qualitatively adequate crop production despite the negative effects of climate change. As it is not yet possible to select for adaptation to future climate scenarios in the field, simulations of future conditions in controlled-environment (CE) phenotyping facilities contribute to the understanding of the plant response to special stress conditions and help breeders to select ideal genotypes which cope with future conditions. CE phenotyping facilities enable the collection of traits that are not easy to measure under field conditions and the assessment of a plant‘s phenotype under repeatable, clearly defined environmental conditions using automated, non-invasive, high-throughput methods. However, extrapolation and translation of results obtained under controlled environments to field environments is ambiguous. This review outlines the opportunities and challenges of phenotyping approaches under controlled environments complementary to conventional field trials. It gives an overview on general principles and introduces existing phenotyping facilities that take up the challenge of obtaining reliable and robust phenotypic data on climate response traits to support breeding of climate-adapted crops.

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Crop Response to Leaf and Seed Applications of the Biostimulant ComCat® under Stress Conditions

Agronomy ◽

10.3390/agronomy11061161 ◽

2021 ◽

Vol 11 (6) ◽

pp. 1161

Author(s):

Roland Gerhards ◽

Fructueuse N. Ouidoh ◽

André Adjogboto ◽

Vodéa Armand Pascal Avohou ◽

Berteulot Latus Sètondji Dossounon ◽

...

Keyword(s):

Seed Treatment ◽

Crop Production ◽

Field Experiments ◽

Nutrient Deficiency ◽

Natural Compounds ◽

Application Rate ◽

Winter Barley ◽

Block Designs ◽

Crop Response ◽

Yield Data

Although clear evidence for benefits in crop production is partly missing, several natural compounds and microorganisms have been introduced to the market as biostimulants. They are supposed to enhance nutrient efficiency and availability in the rhizosphere, reduce abiotic stress, and improve crop quality parameters. Biostimulants often derive from natural compounds, such as microorganisms, algae, and plant extracts. In this study, the commercial plant extract-based biostimulant ComCat® was tested in two field experiments with maize in the communities of Banikoara and Matéri in Northern Benin and six pot experiments (four with maize and two with winter barley) at the University of Hohenheim in Germany. Maize was grown under nutrient deficiency, drought, and weed competition, and winter barley was stressed by the herbicide Luximo (cinmethylin). ComCat® was applied at half, full, and double the recommended field rate (50, 100, and 200 g ha−1) on the stressed and unstressed control plants as leaf or seed treatment. The experiments were conducted in randomized complete block designs with four replications. The above-ground biomass and yield data of one experiment in Benin were collected. The biostimulant did not promote maize and winter barley biomass production of the unstressed plants. When exposed to stress, ComCat@ resulted only in one out of eight experiments in higher barley biomass compared to the stressed treatment without ComCat® application. There was a reduced phytotoxic effect of cinmethylin after seed treatment with ComCat®. Crop response to ComCat® was independent of the application rate. Basic and applied studies are needed to investigate the response of crops to biostimulants and their mechanisms of action in the plants before they should be used in practical farming.

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The interaction of seasonal rainfall and nitrogen fertiliser rate on soil N2O emission, total N loss and crop yield of dryland sorghum and sunflower grown on sub-tropical Vertosols

Soil Research ◽

10.1071/sr15286 ◽

2016 ◽

Vol 54 (5) ◽

pp. 604 ◽

Cited By ~ 23

Author(s):

G. D. Schwenke ◽

B. M. Haigh

Keyword(s):

Crop Yield ◽

Crop Production ◽

Field Experiments ◽

N2o Emission ◽

N2o Emissions ◽

N Loss ◽

N Losses ◽

Total N ◽

Tropical Regions ◽

The Impact

Summer crop production on slow-draining Vertosols in a sub-tropical climate has the potential for large emissions of soil nitrous oxide (N2O) from denitrification of applied nitrogen (N) fertiliser. While it is well established that applying N fertiliser will increase N2O emissions above background levels, previous research in temperate climates has shown that increasing N fertiliser rates can increase N2O emissions linearly, exponentially or not at all. Little such data exists for summer cropping in sub-tropical regions. In four field experiments at two locations across two summers, we assessed the impact of increasing N fertiliser rate on both soil N2O emissions and crop yield of grain sorghum (Sorghum bicolor L.) or sunflower (Helianthus annuus L.) in Vertosols of sub-tropical Australia. Rates of N fertiliser, applied as urea at sowing, included a nil application, an optimum N rate and a double-optimum rate. Daily N2O fluxes ranged from –3.8 to 2734g N2O-Nha–1day–1 and cumulative N2O emissions ranged from 96 to 6659g N2O-Nha–1 during crop growth. Emissions of N2O increased with increased N fertiliser rates at all experimental sites, but the rate of N loss was five times greater in wetter-than-average seasons than in drier conditions. For two of the four experiments, periods of intense rainfall resulted in N2O emission factors (EF, percent of applied N emitted) in the range of 1.2–3.2%. In contrast, the EFs for the two drier experiments were 0.41–0.56% with no effect of N fertiliser rate. Additional 15N mini-plots aimed to determine whether N fertiliser rate affected total N lost from the soil–plant system between sowing and harvest. Total 15N unaccounted was in the range of 28–45% of applied N and was presumed to be emitted as N2O+N2. At the drier site, the ratio of N2 (estimated by difference)to N2O (measured) lost was a constant 43%, whereas the ratio declined from 29% to 12% with increased N fertiliser rate for the wetter experiment. Choosing an N fertiliser rate aimed at optimum crop production mitigates potentially high environmental (N2O) and agronomic (N2+N2O) gaseous N losses from over-application, particularly in seasons with high intensity rainfall occurring soon after fertiliser application.

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Interactive effect of biochar and plant growth-promoting bacterial endophytes on ameliorating salinity stress in maize

Functional Plant Biology ◽

10.1071/fp15054 ◽

2015 ◽

Vol 42 (8) ◽

pp. 770 ◽

Cited By ~ 63

Author(s):

Saqib Saleem Akhtar ◽

Mathias Neumann Andersen ◽

Muhammad Naveed ◽

Zahir Ahmad Zahir ◽

Fulai Liu

Keyword(s):

Plant Growth ◽

Salinity Stress ◽

Crop Production ◽

Interactive Effect ◽

Nutrient Balance ◽

Bacterial Strains ◽

Negative Effects ◽

Plant Growth Promoting ◽

Growth Promoting ◽

Salt Affected Soils

The objective of this work was to study the interactive effect of biochar and plant growth-promoting endophytic bacteria containing 1-aminocyclopropane-1-carboxylate deaminase and exopolysaccharide activity on mitigating salinity stress in maize (Zea mays L.). The plants were grown in a greenhouse under controlled conditions, and were subjected to separate or combined treatments of biochar (0% and 5%, w/w) and two endophytic bacterial strains (Burkholderia phytofirmans (PsJN) and Enterobacter sp. (FD17)) and salinity stress. The results indicated that salinity significantly decreased the growth of maize, whereas both biochar and inoculation mitigated the negative effects of salinity on maize performance either by decreasing the xylem Na+ concentration ([Na+]xylem) uptake or by maintaining nutrient balance within the plant, especially when the two treatments were applied in combination. Moreover, in biochar-amended saline soil, strain FD17 performed significantly better than did PsJN in reducing [Na+]xylem. Our results suggested that inoculation of plants with endophytic baterial strains along with biochar amendment could be an effective approach for sustaining crop production in salt-affected soils.

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