Clinical Significance of p27 and Skp2 Protein Expression in Uterine Cervical Neoplasm

Abstract Background Emerging evidence confirms that lncRNAs (long non-coding RNAs) are potential biomarkers that play vital roles in tumors. ZNF582-AS1 is a novel lncRNA that serves as a potential prognostic marker of cancers. However, the specific clinical significance and molecular mechanism of ZNF582-AS1 in ccRCC (clear cell renal cell carcinoma) are unclear. Methods Expression level and clinical significance of ZNF582-AS1 were determined by TCGA-KIRC data and qRT-PCR results of 62 ccRCCs. DNA methylation status of ZNF582-AS1 promoter was examined by MSP, MassARRAY methylation and demethylation analysis. Gain-of-function experiments were conducted to investigate the biological roles of ZNF582-AS1 in the phenotype of ccRCC. The subcellular localization of ZNF582-AS1 was detected by RNA FISH. iTRAQ, RNA pull-down and RIP-qRT-PCR were used to identify the downstream targets of ZNF582-AS1. rRNA MeRIP-seq and MeRIP-qRT-PCR were utilized to examine the N(6)-methyladenosine modification status. Western blot and immunohistochemistry assays were used to determine the protein expression level. Results ZNF582-AS1 was downregulated in ccRCC, and decreased ZNF582-AS1 expression was significantly correlated with advanced tumor stage, higher pathological stage, distant metastasis and poor prognosis. Decreased ZNF582-AS1 expression was caused by DNA methylation at the CpG islands within its promoter. ZNF582-AS1 overexpression inhibited cell proliferative, migratory and invasive ability, and increased cell apoptotic rate in vitro and in vivo. Mechanistically, we found that ZNF582-AS1 overexpression suppressed the N(6)-methyladenosine modification of MT-RNR1 by reducing rRNA adenine N(6)-methyltransferase A8K0B9 protein level, resulting in the decrease of MT-RNR1 expression, followed by the inhibition of MT-CO2 protein expression. Furthermore, MT-RNR1 overexpression reversed the decreased MT-CO2 expression and phenotype inhibition of ccRCC induced by increased ZNF582-AS1 expression. Conclusions This study demonstrates for the first time that ZNF582-AS1 functions as a tumor suppressor gene in ccRCC and ZNF582-AS1 may serve as a potential biomarker and therapeutic target of ccRCC.

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Clinical significance of gelsolin-like actin-capping protein expression in oral carcinogenesis: an immunohistochemical study of premalignant and malignant lesions of the oral cavity

BMC Cancer ◽

10.1186/1471-2407-8-39 ◽

2008 ◽

Vol 8 (1) ◽

Cited By ~ 27

Author(s):

Hitomi Nomura ◽

Katsuhiro Uzawa ◽

Takashi Ishigami ◽

Yukinao Kouzu ◽

Hirofumi Koike ◽

...

Keyword(s):

Oral Cavity ◽

Protein Expression ◽

Clinical Significance ◽

Immunohistochemical Study ◽

Capping Protein ◽

Oral Carcinogenesis ◽

Actin Capping Protein ◽

Malignant Lesions ◽

Actin Capping

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Clinical significance of Reg IV protein expression in human colorectal cancer

World Chinese Journal of Digestology ◽

10.11569/wcjd.v18.i33.3594 ◽

2010 ◽

Vol 18 (33) ◽

pp. 3594

Author(s):

Sheng-Qin Zheng ◽

Jie He ◽

Pei-Lin Huang ◽

Jian-Hua Wang

Keyword(s):

Colorectal Cancer ◽

Protein Expression ◽

Clinical Significance ◽

Human Colorectal Cancer

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The expression and clinical significance of PDCD4 and Her-2 in human gastric cancer

European Journal of Inflammation ◽

10.1177/2058739219828236 ◽

2019 ◽

Vol 17 ◽

pp. 205873921982823

Author(s):

Yuelou Yang ◽

Xiangjun Jiang ◽

Dong Li ◽

Feiyan Wang ◽

Qun Yang ◽

...

Keyword(s):

Gastric Cancer ◽

Protein Expression ◽

Western Blot ◽

Clinical Significance ◽

Normal Mucosa ◽

Normal Tissues ◽

Positive Rate ◽

Her 2 ◽

Cancer Tissues ◽

Pdcd4 Protein

To investigate the correlation and clinical significance between programmed cell death factor 4 (PDCD4) and epidermal growth factor receptor 2 (Her-2) expressions and clinicopathological parameters in patients with gastric cancer, a total of 65 cases of gastric cancer and the corresponding normal mucosa with PDCD4 and Her-2 protein expressions were detected by SP immunohistochemical staining, and 50 cases of gastric cancer and the corresponding normal mucosa with PDCD4 and Her-2 protein expression quantities were detected by Western blot, in order to analyze the relationship between the positive expressions of PDCD4 and Her-2 protein and the clinicopathological features of patients with gastric cancer. The results showed that the positive rate of PDCD4 protein expression in gastric cancer tissues was 7.7%, which was significantly lower than that in the corresponding normal tissues, that is, 77.5% ( P < 0.05); the positive rate of Her-2 expression was 41.5%, which was significantly higher than that of the corresponding normal tissues, which is 2.5% ( P < 0.05). The Western blot test showed that the expression of PDCD4 protein in gastric cancer was 0.3105 ± 0.0073, which was significantly lower than that in the corresponding normal tissues, that is, 0.9428 ± 0.0127 ( P < 0.05); the expression level of Her-2 protein in gastric cancer tissues was 0.9428 ± 0.0127, which was significantly higher than that of the corresponding normal mucosa, which is 0.2054 ± 0.0264 ( P < 0.05). The positive expressions of PDCD4 (5/65) and Her-2 (27/65) were significantly correlated with the differentiation degrees and TNM stages of gastric cancer ( P < 0.05). However, no significant correlation can be observed from Table 2 ( P > 0.05), regarding sex, age, tumor size, and lymph node metastasis. Our research claimed that PDCD4 and Her-2 may play an important role in the invasion and metastasis of gastric cancer, which has a negative correlation with biological behaviors of gastric cancer. The low expression of PDCD4 and the high expression of Her-2 in gastric cancer may promote the occurrence and progression of cancer. The PDCD4 and Her-2 test can be used as an index to evaluate the malignant biological behaviors of gastric cancer and prognosis, and provide a theoretical basis for targeted therapy.

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Uterine Cervical Neoplasm Diagnosed by Flexible Magnifying Endoscopy with Narrow Band Imaging

Diagnostics ◽

10.3390/diagnostics10110903 ◽

2020 ◽

Vol 10 (11) ◽

pp. 903

Author(s):

Hideki Kobara ◽

Kunihisa Uchita ◽

Noriya Uedo ◽

Noriko Matsuura ◽

Noriko Nishiyama ◽

...

Keyword(s):

Cervical Cancer ◽

Pap Smear ◽

Narrow Band ◽

Narrow Band Imaging ◽

Early Stage ◽

Magnifying Endoscopy ◽

Diagnostic Tools ◽

Gastrointestinal Neoplasms ◽

Cervical Neoplasm ◽

Uterine Cervical Neoplasm

When detected early, uterine cervical cancer is one of the most successfully treatable forms of cancer. The diagnostic accuracy of the standard method, the Pap smear test followed by colposcopy, remains unsatisfactory. To improve detection of early-stage cervical cancer, new diagnostic tools for uterine cervical intraepithelial neoplasm (CIN) need to be developed. Magnifying endoscopy with narrow- band imaging (ME-NBI), which allows the visualization of the micro-structure as well as micro-vascularity of the mucosal surface, has excellent diagnostic ability for early gastrointestinal neoplasms. In our previous investigation, ME-NBI was efficacious for diagnosis of CIN. We herein report two notable cases of CIN3 that were diagnosed by ME-NBI that were not detected by colposcopy. These cases illustrate the usefulness of ME-NBI for diagnosis of early-stage uterine cervical neoplasms.

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Expression and Clinical Significance of ILF2 in Gastric Cancer

Disease Markers ◽

10.1155/2017/4387081 ◽

2017 ◽

Vol 2017 ◽

pp. 1-9 ◽

Cited By ~ 6

Author(s):

Zi-huan Yin ◽

Xing-wang Jiang ◽

Wu-bing Shi ◽

Qian-le Gui ◽

Dong-feng Yu

Keyword(s):

Gastric Cancer ◽

Protein Expression ◽

Clinical Significance ◽

Disease Free Survival ◽

High Expression ◽

Clinicopathological Parameters ◽

Rank Test ◽

Depth Of Invasion ◽

Clinical Prognosis ◽

Expression Levels

The aim of this study is to investigate the expression levels and clinical significance of ILF2 in gastric cancer. The mRNA and protein expression levels of ILF2 were, respectively, examined by quantitative real-time PCR (qRT-PCR) and Western blot from 21 paired fresh frozen GC tissues and corresponding normal gastric tissues. In order to analyze the expression pattern of ILF2 in GC, 60 paired paraffin-embedded GC slides and corresponding normal gastric slides were detected by immunohistochemistry (IHC) assay. The correlation between ILF2 protein expression levels and clinicopathological parameters, overall survival (OS), disease-free survival (DFS), and clinical prognosis were analyzed by statistical methods. Significantly higher levels of ILF2 were detected in GC tissues compared with normal controls at both mRNA and protein level. High expression of ILF2 was tightly correlated with depth of invasion, lymph node metastasis, pathological stage, and histological differentiation. Log-rank test showed that high expression of ILF2 was positively associated with poor clinical prognosis. Multivariate analysis identified that ILF2 was an independent prognostic factor for OS and DFS. Our findings suggest that ILF2 may be a valuable biomarker and a novel potential prognosis predictor for GC patients.

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