scholarly journals Genomic variations of SARS-CoV-2 suggest multiple outbreak sources of transmission

2020 ◽  
Author(s):  
Liangsheng Zhang ◽  
Jian-Rong Yang ◽  
Zhenguo Zhang ◽  
Zhenguo Lin
Keyword(s):  
Phylogenetic Analysis ◽  
Prevention And Control ◽  
Type I ◽  
Type Ii ◽  
Direct Link ◽  
Genomic Variations ◽  
Type Ia ◽  
And Control

SummaryWe examined 169 genomes of SARS-CoV-2 and found that they can be classified into two major genotypes, Type I and Type II. Type I can be further divided into Type IA and IB. Our phylogenetic analysis showed that the Type IA resembles the ancestral SARS-CoV-2 most. Type II was likely evolved from Type I and predominant in the infections. Our results suggest that Type II SARS-CoV-2 was the source of the outbreak in the Wuhan Huanan market and it was likely originated from a super-spreader. The outbreak caused by the Type I virus should have occurred somewhere else, because the patients had no direct link to the market. Furthermore, by analyzing three genomic sites that distinguish Type I and Type II strains, we found that synonymous changes at two of the three sites confer higher protein translational efficiencies in Type II strains than in Type I strains, which might explain why Type II strains are predominant, implying that Type II is more contagious (transmissible) than Type I. These findings could be valuable for the current epidemic prevention and control.

scholarly journals Genetic and Pathogenic Characterization of QX(GI-19)-Recombinant Infectious Bronchitis Viruses in South Korea

Viruses ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1163
Author(s):  
So-Youn Youn ◽  
Ji-Youn Lee ◽  
You-Chan Bae ◽  
Yong-Kuk Kwon ◽  
Hye-Ryoung Kim
Keyword(s):  
Phylogenetic Analysis ◽  
Disease Prevention ◽  
South Korea ◽  
Prevention And Control ◽  
Genetic Recombination ◽  
Detection Methods ◽  
Infectious Bronchitis ◽  
Reference Sequences ◽  
And Control

Infectious bronchitis viruses (IBVs) are evolving continuously via genetic drift and genetic recombination, making disease prevention and control difficult. In this study, we undertook genetic and pathogenic characterization of recombinant IBVs isolated from chickens in South Korea between 2003 and 2019. Phylogenetic analysis showed that 46 IBV isolates belonged to GI-19, which includes nephropathogenic IBVs. Ten isolates formed a new cluster, the genomic sequences of which were different from those of reference sequences. Recombination events in the S1 gene were identified, with putative parental strains identified as QX-like, KM91-like, and GI-15. Recombination detection methods identified three patterns (rGI-19-I, rGI-19-II, and rGI-19-III). To better understand the pathogenicity of recombinant IBVs, we compared the pathogenicity of GI-19 with that of the rGI-19s. The results suggest that rGI-19s may be more likely to cause trachea infections than GI-19, whereas rGI-19s were less pathogenic in the kidney. Additionally, the pathogenicity of rGI-19s varied according to the genotype of the major parent. These results indicate that genetic recombination between heterologous strains belonging to different genotypes has occurred, resulting in the emergence of new recombinant IBVs in South Korea.

scholarly journals Implications of Aerobic Exercises and Walking Parallel to Diabetes Medications on Diabetic Sportspersons

2021 ◽  
pp. 197-204
Author(s):  
Fehmida Ayub ◽  
Abida Naseer ◽  
Saeed Javed ◽  
Adnan Asghar ◽  
Abd Rahim Mohd Shariff ◽  
...  
Keyword(s):  
Aerobic Exercise ◽  
Blood Glucose Control ◽  
Daily Basis ◽  
Control Group ◽  
Type I ◽  
Type Ii ◽  
Glucose Levels ◽  
Spare Time ◽  
And Control ◽  
Experimental Group

Objective: Diabetes have a central contribution with type I or type II towards the healthy lifestyles of sportspersons. Aerobic exercise and daily walking stay them fit and control their glucose levels in their bloodstream. The aim of this study was to find out the effects of aerobic exercises and walk on the sportspersons of type I and II diabetes. Methodology: The existing research has experimental design itself wherein pre-tests and post-tests were employed to make sure the novelty of results. The data was collected from the diabetic sportspersons dividing them equally into control group (N-20) and experimental group (N-20). Both groups had type I (N-20) and type II (N-20) diabetic individuals. Aerobic exercise and walk protocol was applied for six weeks on experimental group, whereas, control group continued their routine activities. Afterwards, the data was collected through pre and post treatments and edited into SPSS (v-26). The collected data was analyzed through descriptive statistics using frequencies and percentages, whereas, T-test was applied to make the differences of pre and post treatments. Results: The findings has shown that aerobic exercises and walk decrease the higher levels of glucose in blood and enable to stable glycemic balance, weight loss maintenance, decrease insulin resistance, blood pressure decrease, and blood glucose control. Conclusion: The prominent differences were observed between control and experimental groups either type I or type II. It was concluded that the sportspersons may reduce the excessive glucose engaging in aerobic exercises and walk on daily basis rather than using medications. They should spend their happy lives and get rid of medications and insulin through spending their spare time using light exercises and maintain their glucose levels in blood as well.

scholarly journals Roles of gene transcription and PKA subtype activation in maturation of murine oocytes

Reproduction ◽  
2002 ◽  
pp. 799-806 ◽  
Author(s):  
KF Rodriguez ◽  
RM Petters ◽  
AE Crosier ◽  
CE Farin
Keyword(s):  
Gene Transcription ◽  
Cumulus Cell ◽  
Cumulus Cells ◽  
Type I ◽  
Type Ii ◽  
Germinal Vesicle Breakdown ◽  
Series Of Experiments ◽  
And Control ◽  
Alpha Amanitin

The aims of this study were to examine the role of transcription and the coincident involvement of type I and type II protein kinase A (PKA) in the resumption of meiosis in murine cumulus-oocyte complexes (COCs) using the transcriptional inhibitors 5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole (DRB) and alpha-amanitin. The first series of experiments was designed to: (i) characterize the role of transcription in gonadotrophin-mediated and spontaneous maturation of murine oocytes; (ii) examine the roles of specific gonadotrophins (FSH versus hCG) and cumulus cells in transcriptionally mediated oocyte maturation; and (iii) determine the reversibility of the transcriptional arrest of meiosis. In the presence of FSH, transcriptional inhibitors arrested germinal vesicle breakdown (GVBD) (DRB: 2 +/- 2% and control: 76 +/- 2%; alpha-amanitin: 4 +/- 4% and control: 70 +/- 4%). Furthermore, cumulus cells were required for transcriptional inhibitors to arrest GVBD (DRB with cumulus cells: 0 +/- 15%; DRB without cumulus cells: 94 +/- 13%; alpha-amanitin with cumulus cells: 15 +/- 2%; alpha-amanitin without cumulus cells: 99 +/- 2%). Thus, in mice, FSH-mediated GVBD uses a transcriptional mechanism, which probably occurs within the cumulus cell compartment. In a second series of experiments, the role of transcription in mediating the resumption of meiosis after activation of either type I or type II PKA was examined. Activation of type I PKA in murine COCs resulted in an arrest of GVBD that was independent of a transcriptional event (with DRB: 7 +/- 9% GVBD; without DRB: 11 +/- 9% GVBD). In contrast, activation of type II PKA resulted in a resumption of meiosis, which required the occurrence of gene transcription (with DRB: 12 +/- 9% GVBD; without DRB: 80 +/- 9% GVBD). As FSH binding to cumulus cells activates the PKA second messenger system, our results indicate that, in cultured murine COCs, FSH binding to cumulus cells results in the activation of type II PKA, which, in turn, mediates a downstream transcriptional event required for the initiation of GVBD.

Some large classes of n-valued Sheffer functions

1973 ◽  
Vol 74 (2) ◽  
pp. 201-211 ◽  
Author(s):  
J. C. Muzio
Keyword(s):  
Prime Factor ◽  
Type I ◽  
Type Ii ◽  
Large Classes ◽  
Type Iii ◽  
Type Ia ◽  
Sheffer Function

In 1967, Singer (11) gave 3 classes of n-valued two-place functors and proved that all these functors were Sheffer functions. Out of the n possible assignments needed to define a functor completely, Singer showed that it was sufficient to define 3n − 2, 3n − 2, and 2n assignments respectivelyfor the 3 classes. We shall enlarge Singer's classes to give functors of type Ia, type II and type III. For types Ia and III, it will be shown that it is sufficient to define 2n − 1 assignments and for type II we require 2n − 1 assignments to be defined and conditions on a further n/p1 assignments (where P1 is the least prime factor of n). These classes of functors include all of Singer's classes. We also introduce functors of type Ib, similar to those of type Ia, and show that for these itis sufficient to define 2n − 1 assignments to ensure the functor is a Sheffer function.

scholarly journals Is there a relationship between Lund-Mackay scale, olfactory bulb depth and width, and Keros classification in patients with nasal polyps?

2021 ◽  
Vol 11 (44) ◽  
pp. 167-173
Author(s):  
Ziya Şencan ◽  
Nuray Bayar Muluk ◽  
Mikail Inal ◽  
Selmin Perihan Kömürcü Erkmen ◽  
Ela Cömert
Keyword(s):  
Olfactory Bulb ◽  
Healthy Subjects ◽  
Older Patients ◽  
Nasal Polyps ◽  
Control Group ◽  
Type I ◽  
Type Ii ◽  
Positive Correlations ◽  
And Control ◽  
The Relationship

Abstract OBJECTIVE. We investigated the relationship between Lund-Mackay scale, olfactory bulb depth and width, and Keros classification in patients with chronic rhinosinusitis with nasal polyps (CRSwNP). MATERIAL AND METHODS. In this retrospective study, paranasal sinus computed tomography (PNSCT) images of 47 patients with CRSwNP and 47 healthy subjects (control) were evaluated. In the CRSwNP group, PNSCT scans were assessed based on Lund-Mackay scale. In both groups, olfactory fossae (OF) depth and width, and Keros classification were evaluated. RESULTS. The total Lund-Mackay score was 17.1±5.9. There were no significant differences between OF depth and width values of the nasal polyps group and control group. For both groups, Type II Keros was the most detected type; secondly, Keros type I and rarely Keros type III were detected. There was no significant correlation between Lund-Mackay score (All items and total score) and OF depth and width, and Keros type. There were negative correlations between ipsilateral OF depth and width (p<0.05), whereas there were positive correlations between contralateral OF depth and width (p>0.05). Keros type was positively correlated between ipsilateral and contralateral OF depth and Keros type (p<0.05). In older patients, left OF depth and Keros type decreased (p<0.05). CONCLUSION. As a conclusion, there was no correlation between Lund-Mackay score and olfactory fossa dimensions (depth and width). When considering age, one could notice that Keros type decreased in older patients.

scholarly journals Ruby Deposits: A Review and Geological Classification

Minerals ◽  
2020 ◽  
Vol 10 (7) ◽  
pp. 597 ◽  
Author(s):  
Gaston Giuliani ◽  
Lee Groat ◽  
Anthony Fallick ◽  
Isabella Pignatelli ◽  
Vincent Pardieu
Keyword(s):  
Democratic Republic Of Congo ◽  
Granulite Facies ◽  
Sensu Stricto ◽  
Classification Systems ◽  
Type I ◽  
Type Ii ◽  
Carbonate Platforms ◽  
Alkali Basalts ◽  
Rock Interaction ◽  
Type Ia

Corundum is not uncommon on Earth but the gem varieties of ruby and sapphire are relatively rare. Gem corundum deposits are classified as primary and secondary deposits. Primary deposits contain corundum either in the rocks where it crystallized or as xenocrysts and xenoliths carried by magmas to the Earth’s surface. Classification systems for corundum deposits are based on different mineralogical and geological features. An up-to-date classification scheme for ruby deposits is described in the present paper. Ruby forms in mafic or felsic geological environments, or in metamorphosed carbonate platforms but it is always associated with rocks depleted in silica and enriched in alumina. Two major geological environments are favorable for the presence of ruby: (1) amphibolite to medium pressure granulite facies metamorphic belts and (2) alkaline basaltic volcanism in continental rifting environments. Primary ruby deposits formed from the Archean (2.71 Ga) in Greenland to the Pliocene (5 Ma) in Nepal. Secondary ruby deposits have formed at various times from the erosion of metamorphic belts (since the Precambrian) and alkali basalts (from the Cenozoic to the Quaternary). Primary ruby deposits are subdivided into two types based on their geological environment of formation: (Type I) magmatic-related and (Type II) metamorphic-related. Type I is characterized by two sub-types, specifically Type IA where xenocrysts or xenoliths of gem ruby of metamorphic (sometimes magmatic) origin are hosted by alkali basalts (Madagascar and others), and Type IB corresponding to xenocrysts of ruby in kimberlite (Democratic Republic of Congo). Type II also has two sub-types; metamorphic deposits sensu stricto (Type IIA) that formed in amphibolite to granulite facies environments, and metamorphic-metasomatic deposits (Type IIB) formed via high fluid–rock interaction and metasomatism. Secondary ruby deposits, i.e., placers are termed sedimentary-related (Type III). These placers are hosted in sedimentary rocks (soil, rudite, arenite, and silt) that formed via erosion, gravity effect, mechanical transport, and sedimentation along slopes or basins related to neotectonic motions and deformation.

A new classification and strategies for endovascular treatment of celiac artery aneurysms

Vascular ◽  
2021 ◽  
pp. 170853812110327
Author(s):  
Xu Li ◽  
Wan Zhang ◽  
Min Zhou ◽  
Yong Ding ◽  
Yonggang Wang ◽  
...  
Keyword(s):  
Endovascular Treatment ◽  
Clinical Manifestations ◽  
Treatment Strategies ◽  
Celiac Artery ◽  
Type I ◽  
Type Ii ◽  
Main Trunk ◽  
Single Center Experience ◽  
Type Ia

Background Endovascular treatment is being increasingly used for celiac artery aneurysms (CAAs), but systematic endovascular treatment strategies have not been defined yet. This study intended to investigate the strategies of endovascular management of CAAs according to a single-center experience. Methods Anatomically, CAAs were classified into two types: Type I CAAs located in the main trunk of celiac artery. Type II CAAs located on the branches of the celiac artery. Type I and Type II CAAs can be further divided into two different subtypes according to fusiform (a) or saccular or (b) morphology: type Ia, type Ib, type IIa, and type IIb. Patient demographics, clinical manifestations, aneurysm characteristics, endovascular intervention procedures, and perioperative and follow-up outcomes were reviewed and analyzed. Results Between August 2012 and August 2020, 18 consecutive patients (12 men; mean age, 56.8 ± 14.5 years) with CAAs were identified and treated with endovascular procedures. There were seven patients with type Ia, three patients with type Ib, four patients with type IIa, and four patients with type IIb CAAs. One patient died of hemorrhagic shock due to a ruptured aneurysm. Technical success was achieved in 16 patients (88.9%). The mean follow-up period was 51.7 ± 19.4 months. No hepatic or intestinal ischemia or death developed perioperatively or during the follow-up period. No aneurysmal expansion was detected on CTA surveillance, except for one patient who was diagnosed with an endoleak during the follow-up and received reintervention. Conclusions The endovascular strategy based on the novel classification of CAAs was safe and effective, with a favorable mid-term clinical outcome.

scholarly journals Monopolar and Bipolar Combination Injuries of the Clavicle: Retrospective Incidence Analysis and Proposal of a New Classification System

2021 ◽  
Vol 10 (24) ◽  
pp. 5764
Author(s):  
Mustafa Sinan Bakir ◽  
Roman Carbon ◽  
Axel Ekkernkamp ◽  
Stefan Schulz-Drost
Keyword(s):  
Case Reports ◽  
Clavicle Fracture ◽  
Type I ◽  
Type Ii ◽  
New Classification ◽  
Joint Dislocations ◽  
Type Ia ◽  
Icd 10 ◽  
Bipolar Type ◽  
Incidence Analysis

Clavicle injuries are common, but only few case reports describe combined clavicular injuries (CCI). CCI include combinations between clavicular fractures and acromioclavicular/sternoclavicular joint dislocations (SCJD). We present the first general therapeutic recommendations for CCI based on a new classification and their distribution. A retrospective, epidemiological, big data analysis was based on ICD-10 diagnoses from 2012 to 2014 provided by the German Federal Statistical Office. CCI represent 0.7% of all clavicle-related injuries (n = 814 out of 114,003). SCJD show by far the highest proportion of combination injuries (13.2% of all SCJD were part of CCI) while the proportion of CCI in relation to the other injury entities was significantly less (p < 0.023). CCIs were classified depending on (1) the polarity (monopolar type I, 92.2% versus bipolar type II, 7.8%). Monopolar type I was further differentiated depending on (2) the positional relationship between the combined injuries: Ia two injuries directly at the respective pole versus Ib with an injury at one end plus an additional midshaft clavicle fracture. Type II was further differentiated depending on (3) the injured structures: IIa ligamento-osseous, type IIb purely ligamentous (rarest with 0.6%). According to our classification, the CCI severity increases from type Ia to IIb. CCI are more important than previously believed and seen as an indication for surgery. The exclusion of further, contra-polar injuries in the event of a clavicle injury is clinically relevant and should be focused.

scholarly journals Ocular Lesions in Red-Tailed Hawks (Buteo jamaicensis) With Naturally Acquired West Nile Disease

2016 ◽  
Vol 54 (2) ◽  
pp. 277-287 ◽  
Author(s):  
A. Wünschmann ◽  
A. G. Armién ◽  
M. Khatri ◽  
L. C. Martinez ◽  
M. Willette ◽  
...  
Keyword(s):  
Terminal Deoxynucleotidyl Transferase ◽  
Glial Fibrillary Acid Protein ◽  
Type I ◽  
Type Ii ◽  
Outer Retina ◽  
West Nile ◽  
Retinal Lesions ◽  
Acid Protein ◽  
Ocular Lesions ◽  
Type Ia

Ocular lesions are common in red-tailed hawks with West Nile (WN) disease. These lesions consist of pectenitis, choroidal or retinal inflammation, or retinal necrosis, but detailed investigation of the ocular lesions is lacking. Postmortem examination of the eyes of 16 red-tailed hawks with naturally acquired WN disease and 3 red-tailed hawks without WN disease was performed using histopathology, immunohistochemistry for West Nile virus (WNV) antigen, glial fibrillary acid protein, cleaved caspase-3, and the terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling method. Retinal lesions were classified as type I or type II lesions. Type I lesions were characterized by lymphoplasmacytic infiltrates in the subjacent choroid with degeneration limited to the outer retina (type Ia lesion) or with degeneration and necrosis of the outer retina or outer and inner retina (type Ib lesion) while retinal collapse, atrophy, and scarring were hallmarks of type II lesions. Type II retinal lesions were associated with a more pronounced choroiditis. Although not statistically significant, WNV antigen tended to be present in larger quantity in type Ib lesions. Type I lesions are considered acute while type II lesions are chronic. The development of retinal lesions was associated with the presence of an inflammatory infiltrate in the choroid. A breakdown of the blood-retina barrier is suspected to be the main route of infection of the retina. Within the retina, virus appeared to spread via both neuronal and Müller cell processes.

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