scholarly journals Pangenome of white lupin provides insights into the diversity of the species

2020 ◽  
Author(s):  
Bárbara Hufnagel ◽  
Alexandre Soriano ◽  
Jemma Taylor ◽  
Fanchon Divol ◽  
Magdalena Kroc ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Nutritional Value ◽  
Lupinus Albus ◽  
Reference Sequence ◽  
White Lupin ◽  
Plant Protein Sources ◽  
Modern Breeding ◽  
Recent Sequencing ◽  
Core Genes ◽  
Comprehensive Reference

ABSTRACTBackgroundWhite lupin is an old crop with renewed interest due to its seed high protein content and high nutritional value. Despite a long domestication history in the Mediterranean basin, modern breeding efforts have been fairly scarce. Recent sequencing of its genome has provided tools for further description of genetic resources but detailed characterization is still missing.ResultsHere, we report the genome sequencing of several accessions that were used to establish a white lupin pangenome. We defined core genes that are present in all individuals and variable genes that are absent in some and may represent a gene pool for stress adaptation. We believe that the identification of novel genes, together with a more comprehensive reference sequence, represents a significant improvement of the white lupin genetic resources. As an example, we used this pangenome to identify selection footprints and to provide a candidate gene for one of the main QTLs associated with late flowering in Ethiopian lupin types. A 686 nucleotide deletion was identified in exon 3 of the LaFTa1 (Lupinus albus Flowering Time a1) gene that suggests a molecular origin for this trait of importance, defining the need for vernalization in some lupins.ConclusionsThe white lupin pangenome provides a novel genetic resource to better understand how domestication has shaped the genomic variability amongst this crop. It will be of major importance for breeders to select new breeding traits and incorporate them into new, more efficient and robust cultivars in order to face a growing demand for plant protein sources, notably in Europe.

CRUDE PROTEIN, AMINO ACID AND ALKALOID CONTENTS OF ANNUAL SWEET LUPIN (LUPINUS SPP. L.) FORAGES AND SEEDS GROWN IN ETHIOPIA

2012 ◽  
Vol 48 (3) ◽  
pp. 414-427 ◽  
Author(s):  
LIKAWENT YEHEYIS ◽  
C. KIJORA ◽  
E. VAN SANTEN ◽  
M. WINK ◽  
JÜRGEN DANIER ◽  
...  
Keyword(s):  
Amino Acid ◽  
Protein Content ◽  
Crude Protein ◽  
Nutritional Value ◽  
Lupinus Albus ◽  
White Lupin ◽  
Crude Protein Content ◽  
Protein Amino Acid ◽  
Livestock Feed ◽  
Alkaloid Contents

SUMMARYThough bitter white lupin (Lupinus albus L.) is a traditional crop in Ethiopia, sweet lupins are new to the country. As a result, the nutritional value of low-alkaloid lupins has not been evaluated under Ethiopian conditions. Crude protein, amino acid and alkaloid contents of 16 cultivars of three annual lupin species grown in four lupin growing locations (Merawi, Finoteselam, Kossober-1 and Kossober-2) of Ethiopia were evaluated. Location × cultivar interaction was a significant source of variation for all traits (p < 0.0001). In all locations, blue entries had either similar (p ≥ 0.0584) or higher (p ≤ 0.0235) forage crude protein content than the Local Landrace, white group and yellow entry. Compared with the Local Landrace, white and blue entries, the sole yellow entry had higher (p ≤ 0.0148) seed crude protein content at all locations except at Kossober-2, where it had similar (p = 0.8460) crude protein content as white entries. The Local Landrace had the highest forage and seed alkaloid contents. However, sweet blue Vitabor and Sanabor entries had the lowest forage and seed alkaloid contents, respectively. Low alkaloid and higher crude protein contents of sweet lupins grown in Ethiopia show the possibility to use sweet lupin forage and seeds as cheap home-grown protein source for livestock feed and human food in the country. However, for more reliable information, the laboratory results need to be verified by animal and human evaluations of the crop.

Adaptation, diversity, and exploitation of global white lupin (Lupinus albus L.) landrace genetic resources

2010 ◽  
Vol 119 (1) ◽  
pp. 114-124 ◽  
Author(s):  
Paolo Annicchiarico ◽  
Nathalie Harzic ◽  
Antonio Melchiorre Carroni
Keyword(s):  
Genetic Resources ◽  
Lupinus Albus ◽  
White Lupin ◽  
Lupinus Albus L

scholarly journals Development of 50 InDel-based barcode system for genetic identification of tartary buckwheat resources

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0250786
Author(s):  
Hwang-Bae Sohn ◽  
Su-Jeong Kim ◽  
Su-Young Hong ◽  
Sin-Gi Park ◽  
Dong-Ha Oh ◽  
...  
Keyword(s):  
Genetic Resources ◽  
Genetic Identification ◽  
Ex Situ ◽  
Tartary Buckwheat ◽  
Reference Sequence ◽  
Fagopyrum Tataricum ◽  
Indel Markers ◽  
Pcr Products ◽  
User Friendly ◽  
Modern Breeding

Tartary buckwheat (Fagopyrum tataricum Gartn.) is a highly functional crop that is poised to be the target of many future breeding efforts. The reliable ex situ conservation of various genetic resources is essential for the modern breeding of tartary buckwheat varieties. We developed PCR-based co-dominant insertion/deletion (InDel) markers to discriminate tartary buckwheat genetic resources. First, we obtained the whole genome from 26 accessions across a superscaffold-scale reference genome of 569.37 Mb for tartary buckwheat cv. “Daegwan 3–7.” Next, 171,926 homogeneous and 53,755 heterogeneous InDels were detected by comparing 26 accessions with the “Daegwan 3–7” reference sequence. Of these, 100 candidate InDels ranging from 5–20 bp in length were chosen for validation, and 50 of them revealed polymorphisms between the 26 accessions and “Daegwan 3–7.” The validated InDels were further tested through the assessment of their likelihood to give rise to a single or a few PCR products in 50 other accessions, covering most tartary buckwheat genome types. The major allele frequencies ranged from 0.5616 at the TB42 locus to 0.9863 at the TB48 locus, with the average PIC value of 0.1532 with a range of 0.0267–0.3712. To create a user-friendly system, the homology of the genotypes between and among the accessions were visualized in both one- (1D) and two-dimensional (2D) barcode types by comparing amplicon polymorphisms with the reference variety, “Daegwan 3–7.” A phylogenetic tree and population structure of the 76 accessions according to amplicon polymorphisms for the 50 InDel markers corresponded to those using non-synonymous single nucleotide polymorphism variants, indicating that the barcode system based on the 50 InDels was a useful tool to improve the reliability of identification of tartary buckwheat accessions in the germplasm stocks.

scholarly journals Quantitative Control of Early Flowering in White Lupin (Lupinus albus L.)

2021 ◽  
Vol 22 (8) ◽  
pp. 3856
Author(s):  
Sandra Rychel-Bielska ◽  
Anna Surma ◽  
Wojciech Bielski ◽  
Bartosz Kozak ◽  
Renata Galek ◽  
...  
Keyword(s):  
Flowering Time ◽  
Association Studies ◽  
Lupinus Albus ◽  
Genotypic Diversity ◽  
Early Flowering ◽  
White Lupin ◽  
Genome Wide Association Studies ◽  
Interacting Protein ◽  
Major Qtls ◽  
Lupinus Albus L

White lupin (Lupinus albus L.) is a pulse annual plant cultivated from the tropics to temperate regions for its high-protein grain as well as a cover crop or green manure. Wild populations are typically late flowering and have high vernalization requirements. Nevertheless, some early flowering and thermoneutral accessions were found in the Mediterranean basin. Recently, quantitative trait loci (QTLs) explaining flowering time variance were identified in bi-parental population mapping, however, phenotypic and genotypic diversity in the world collection has not been addressed yet. In this study, a diverse set of white lupin accessions (n = 160) was phenotyped for time to flowering in a controlled environment and genotyped with PCR-based markers (n = 50) tagging major QTLs and selected homologs of photoperiod and vernalization pathway genes. This survey highlighted quantitative control of flowering time in white lupin, providing statistically significant associations for all major QTLs and numerous regulatory genes, including white lupin homologs of CONSTANS, FLOWERING LOCUS T, FY, MOTHER OF FT AND TFL1, PHYTOCHROME INTERACTING FACTOR 4, SKI-INTERACTING PROTEIN 1, and VERNALIZATION INDEPENDENCE 3. This revealed the complexity of flowering control in white lupin, dispersed among numerous loci localized on several chromosomes, provided economic justification for future genome-wide association studies or genomic selection rather than relying on simple marker-assisted selection.

scholarly journals Overcoming limitations to environmental DNA studies: A coastal temperate reference sequence database for multiple chloroplast gene regions generated in a single assay.

2021 ◽  
Author(s):  
Nicole Foster ◽  
Kor-jent Dijk ◽  
Ed Biffin ◽  
Jennifer Young ◽  
Vicki Thomson ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Dna Barcode ◽  
Environmental Dna ◽  
Reference Sequence ◽  
Reference Database ◽  
Chloroplast Gene ◽  
Coastal Plants ◽  
Reference Databases ◽  
Targeted Capture ◽  
Comprehensive Reference

A proliferation in environmental DNA (eDNA) research has increased the reliance on reference sequence databases to assign unknown DNA sequences to known taxa. Without comprehensive reference databases, DNA extracted from environmental samples cannot be correctly assigned to taxa, limiting the use of this genetic information to identify organisms in unknown sample mixtures. For animals, standard metabarcoding practices involve amplification of the mitochondrial Cytochrome-c oxidase subunit 1 (CO1) region, which is a universally amplifyable region across majority of animal taxa. This region, however, does not work well as a DNA barcode for plants and fungi, and there is no similar universal single barcode locus that has the same species resolution. Therefore, generating reference sequences has been more difficult and several loci have been suggested to be used in parallel to get to species identification. For this reason, we developed a multi-gene targeted capture approach to generate reference DNA sequences for plant taxa across 20 target chloroplast gene regions in a single assay. We successfully compiled a reference database for 93 temperate coastal plants including seagrasses, mangroves, and saltmarshes/samphire’s. We demonstrate the importance of a comprehensive reference database to prevent species going undetected in eDNA studies. We also investigate how using multiple chloroplast gene regions impacts the ability to discriminate between taxa.

scholarly journals EVALUATION OF NUTRITIONAL VALUE OF GRAIN-HAYLAGE FROM LUPIN, OAT AND SUDAN GRASS

2020 ◽  
Vol 2020 (1) ◽  
pp. 63-69
Author(s):  
Valentina Ruckaya ◽  
Alexander Sorokin ◽  
Elena Afonina ◽  
Natal'ya ZAYCEVA
Keyword(s):  
Crude Protein ◽  
Dry Matter ◽  
Nutritional Value ◽  
Single Species ◽  
Matter Content ◽  
Metabolic Energy ◽  
White Lupin ◽  
Dry Matter Content ◽  
Sudan Grass ◽  
Sowing Period

The average data for 2018–2019 are presented for yield, dry matter content in grain-haylage mass, nutritional value of test grain-haylage samples produced from plant mass of white lupin, oat and Sudan grass from single and mixed cenosis of different sowing times. The grain-haylage mass was harvested in the phase of brilliantly ripening lupin bean, milky-waxy ripeness of oats and the end of flowering of the Sudan grass. It’s revealed that variants of the second sowing date — 2 decade of May — have the best yield of grain-haylage mass and its dry matter content. The yield of grain-haylage mass in the best variants of the second sowing period exceeded by 7–8 t/ha. According to the nutritional value of grain silage samples (the content of metabolic energy, crude protein, crude ash and carotene), the best results were obtained in most variants of the first sowing period. In content of the metabolic energy and crude protein of 1 kg of dry matter of grain-haylage, the best were variants of the single-species cenosis of lupine and its mixture with oats of the first sowing period. Variants of single-species sowing of lupine and Sudan grass were distinguished by the content of carotene.

scholarly journals LaPT2 Gene Encodes a Flavonoid Prenyltransferase in White Lupin

2021 ◽  
Vol 12 ◽  
Author(s):  
Jinyue Liu ◽  
Yaying Xia ◽  
Wenbo Jiang ◽  
Guoan Shen ◽  
Yongzhen Pang
Keyword(s):  
Plant Defense ◽  
Human Health ◽  
Fluorescent Protein ◽  
Lupinus Albus ◽  
Yeast Cells ◽  
White Lupin ◽  
In Planta ◽  
Strong Activity ◽  
Prenylated Flavonoids ◽  
Flavonoid Compounds

Legume plants are rich in prenylated flavonoid compounds, which play an important role in plant defense and human health. In the present study, we identified a prenyltransferase (PT) gene, named LaPT2, in white lupin (Lupinus albus), which shows a high identity and close relationship with the other known PT genes involved in flavonoid prenylation in planta. The recombinant LaPT2 protein expressed in yeast cells exhibited a relatively strong activity toward several flavonols (e.g., kaempferol, quercetin, and myricetin) and a relatively weak activity toward flavanone (naringenin). In addition, the recombinant LaPT2 protein was also active toward several other types of flavonoids, including galangin, morin, 5-deoxyquercetin, 4'-O-methylkaempferol, taxifolin, and aromadendrin, with distinct enzymatic affinities. The LaPT2 gene was preferentially expressed in the roots, which is consistent with the presence of prenylated flavonoid kaempferol in the roots. Moreover, we found that the expression level of LaPT2 paralleled with those of LaF3H1 and LaFLS2 genes that were relatively higher in roots and lower in leaves, suggesting that they were essential for the accumulation of prenylated flavonoid kaempferol in roots. The deduced full-length LaPT2 protein and its signal peptide fused with a green fluorescent protein (GFP) are targeted to plastids in the Arabidopsis thaliana protoplast. Our study demonstrated that LaPT2 from white lupin is responsible for the biosynthesis of prenylated flavonoids, in particular flavonols, which could be utilized as phytoalexin for plant defense and bioactive flavonoid compounds for human health.

scholarly journals The impact of sowingdate and production area on the yield of white lupin (Lupinus albus L.) on Nyírség brown forest soil

2014 ◽  
pp. 133-137
Author(s):  
Gabriella Tóth ◽  
Ferenc Borbély
Keyword(s):  
Yield Components ◽  
Crop Yields ◽  
Lupinus Albus ◽  
Meteorological Conditions ◽  
White Lupin ◽  
Seed Number ◽  
Brown Forest Soil ◽  
Production Area ◽  
The Impact ◽  
Lupinus Albus L

The lupine is very sensitive to the different ecological conditions. The examinations of lupine was started in 2003 and our aim is determine yield components which directly affecting crop yields (flower, pod and seed number per plants) in different sowing times (3 times, two weeks apart) and growing area area (240, 480, 720 cm2) combinations. According to our results the sowing times, the growing area and the meteorological conditions are influence on yield significantly. Our data suggest that the early sowing and large growing area combination is favourable to rate of fertilized plants and to development of yield. Later sowing reduces the seed yield depending on the cropyear. In our experiment, the decrease of yield was in the unfavourable year (2003) 20–96%, and in the most favourable meteorological conditions (2004) 10–79%, and in rich rainfall year (2005) 15–88%.

Sign in / Sign up

Export Citation Format

Share Document