Escherichia coli antimicrobial resistance; Phenotypic and genotypic characterization

AbstractImproper use of the antimicrobials as E. coli giving the microorganism multi-resistance against many antimicrobials by gene mutation on integrons, transposons and plasmids. Therefore, our aim in this study is to 1)examine antibiotics resistance phenotype and genotype in Escherichia coli, 2) identifying the structure of bacterial resistance genes on whole-genome sequencing against multi-drug resistant of Escherichia coli in marketed poultry meat. Samples collected, prepared and Bacteriological examination, Antimicrobial sensitivity test performed, Serological identification of Escherichia coli isolates. Results declared that; the prevalence of E. coli from tested chicken meat samples of 100 chicken meat samples surveyed against E. coli the result declared that about 40%. Antimicrobial susceptibility was; antibiotics of choice against E. coli Sulfonamides, Cephalosporins,Tetracyclines, Quinolones. Serologically, STEC (O157:H7) 30%, ETEC (O142) 10%, EHEC (O26:H11). The subunit B of shiga-like toxin (SLT) gene appeared as a homogenous band. Heat-labile toxin (LT) gene was screened in both genomic DNA and plasmid preps in tested strains. Control STEC as it represents a danger to the poultry consumers. We recommended to increase the hygienic measures during slaughtering, processing and/or handling of chicken carcasses and avoidance unnecessary usage of any antimicrobials to avoid appearance of new antimicrobials resistant.

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Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

10.21203/rs.3.rs-72382/v1 ◽

2020 ◽

Author(s):

Thang Nam Nguyen ◽

Diep Thi Khong ◽

Ha Viet Le ◽

Hoa Thi Tran ◽

Quang Ngoc Phan ◽

...

Keyword(s):

Escherichia Coli ◽

Food Contamination ◽

Chicken Meat ◽

Resistant Bacteria ◽

Meat Sample ◽

E Coli ◽

Drug Resistant Bacteria ◽

The Status ◽

Retail Meat ◽

Meat Samples

Abstract Objective: The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the level of resistant bacteria in food, have not yet been well elucidated.Results: In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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Quantitative Analysis of Colistin-Resistant Escherichia Coli in Retail Meat from Local Vietnamese Markets

10.21203/rs.3.rs-72382/v2 ◽

2020 ◽

Author(s):

Thang Nam Nguyen ◽

Diep Thi Khong ◽

Ha Viet Le ◽

Hoa Thi Tran ◽

Quang Ngoc Phan ◽

...

Keyword(s):

Escherichia Coli ◽

Food Contamination ◽

Chicken Meat ◽

Resistant Bacteria ◽

Meat Sample ◽

E Coli ◽

Drug Resistant Bacteria ◽

The Status ◽

Retail Meat ◽

Meat Samples

Abstract The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 104 and 4.2 × 103 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli were a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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Prevalence of Shiga toxin-producing Escherichia coli isolated from chicken meat in west of Iran

10.21203/rs.2.11448/v1 ◽

2019 ◽

Author(s):

omid zarei ◽

Leili Shokoohizadeh ◽

Hadi Hossainpour ◽

Mohammad Yousef Alikhani

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Genetic Relatedness ◽

Culture Media ◽

Chicken Meat ◽

Diffusion Method ◽

Disk Diffusion Method ◽

E Coli ◽

Meat Samples ◽

High Level

Abstract Objective: Shiga toxin producing Escherichia coli (STEC) has known as a crucial zoonotic food borne pathogen. A total of 257 row chicken meat samples were collected from different markets in Hamadan city from January 2016 to May 2017. Samples were cultured on selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by disk diffusion method. The genetic relatedness of STEC isolates were analyzed by ERIC-PCR. Results: Totally, 93(36%) of isolates were identified as E. coli in this current study. According serological and microbiological tests, 5(5.3%), 31(33.3%) and 7(7.5%) of E. coli isolates, characterized as Enterohemorrhagic E. coli (EHEC), STEC and attaching and effacing E. coli (AEEC) strains, respectively. High level resistance to tetracycline (89.8), ampicillin (82.8%) and sulfametoxazole-trimotoprime (71%) were detected among E. coli isolates. Analysis of ERIC-PCR results showed five different ERIC types among EHEC isolates. Based on our findings, chicken meat identified as a sources of STEC strains, therefore, the controlling and checkup the chicken meats for the resistance and virulent strains of E. coli should be consider as a crucial issues in public health.

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Prevalence of Salmonella Isolates and Antimicrobial Resistance in Poultry Meat from South Korea

Journal of Food Protection ◽

10.4315/0362-028x.jfp-14-018 ◽

2014 ◽

Vol 77 (9) ◽

pp. 1579-1582 ◽

Cited By ~ 11

Author(s):

RAN-HEE YOON ◽

SE-YEOUN CHA ◽

BAI WEI ◽

JAE-HEE ROH ◽

HYE-SUK SEO ◽

...

Keyword(s):

South Korea ◽

Foodborne Pathogens ◽

Salmonella Enteritidis ◽

Chicken Meat ◽

Poultry Meat ◽

Antibiotics Resistance ◽

Shopping Malls ◽

Internet Shopping ◽

Traditional Markets ◽

Meat Samples

Contamination of Salmonella was assessed in duck and chicken meat collected from supermarkets, traditional markets, internet shopping malls, and wholesale markets in Jeonlado, South Korea, in 2013. Salmonella contamination was found in 51.3% of duck meat samples and 3.7% of chicken meat samples. Salmonella contamination of duck meat samples differed by meat type, i.e., 69.8% of samples of whole ducks and 33.9% of samples of duck pieces. Six serotypes were identified from 64 Salmonella isolates in duck meat: Salmonella Typhimurium (37.5%), Salmonella Enteritidis (21.8%), Salmonella Stanley (3.1%), Salmonella Regent (1.6%), Salmonella Winterthur (3.1%), and Salmonella Westhampton (1.6%). All isolates were resistant to one or more antibiotics. Resistance to sulfisoxazole was most common (93.8% of isolates), followed by resistance to nalidixic acid (59.4%), ceftazidime (26.6%), and ampicillin (26.6%). To our knowledge, this study is the first to report Salmonella contamination in duck meat from Korea. Duck meat should be considered an important source of foodborne pathogens.

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The Prevalence of Shiga Toxin-Producing Escherichia coli and Enteropathogenic Escherichia coli Isolated from Raw Chicken Meat Samples

International Journal of Microbiology ◽

10.1155/2021/3333240 ◽

2021 ◽

Vol 2021 ◽

pp. 1-5

Author(s):

Omid Zarei ◽

Leili Shokoohizadeh ◽

Hadi Hossainpour ◽

Mohammad Yousef Alikhani

Keyword(s):

Escherichia Coli ◽

Shiga Toxin ◽

Genetic Relatedness ◽

Culture Media ◽

Chicken Meat ◽

Diffusion Method ◽

Disk Diffusion Method ◽

E Coli ◽

Meat Samples ◽

High Level

Background. Shiga toxin-producing Escherichia coli (STEC) is known as a crucial zoonotic food-borne pathogen. A total of 257 raw chicken meat samples were collected from different markets in Hamadan, west of Iran, from January 2016 to May 2017. Materials and Methods. The samples were cultured in selective and differential culture media, and the virulence genes of E. coli isolates were analyzed by PCR assay. The antibiotic resistance patterns of E. coli isolates were determined by the disk diffusion method. The genetic relatedness of the E. coli O157 isolates was analyzed by ERIC-PCR. Results. In total, 93 (36% ± 3.12) of the isolates were identified as E. coli in this study. Based on serological and microbiological tests, 36 (38.7% ± 9.9), 7 (7.5% ± 5.35), and 12 (12.9% ± 6.81) of the E. coli isolates were characterized as STEC, enteropathogenic E. coli (EPEC), and attaching and effacing E. coli (AEEC) strains, respectively. A high level of resistance to nalidixic acid (91.4% ± 5.7), tetracycline (89.2% ± 6.31), ampicillin (82.8% ± 7.67), and trimotoprime-sulfametoxazole (71% ± 9.22) was detected among the E. coli isolates. The analysis of the ERIC-PCR results showed five different ERIC types among the E. coli O157 isolates. Conclusions. Based on our findings, control and check-up of poultry meats should be considered as a crucial issue for public health.

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Determination of the ciprofloxacin-resistant Escherichia coli isolated from chicken meat in Turkey

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.25162 ◽

2020 ◽

Vol 71 (3) ◽

pp. 2291

Author(s):

S. SAHIN

Keyword(s):

Escherichia Coli ◽

Public Health Problem ◽

Multidrug Resistant ◽

Chicken Meat ◽

Chain Reactions ◽

Extended Spectrum Beta Lactamase ◽

E Coli ◽

Polymerase Chain Reactions ◽

Resistance Patterns ◽

Meat Samples

In this study, the occurrence of the ciprofloxacin-resistant (CR) Escherichia coli in chicken meat was determined, and their clonal relations were investigated by using pulsed-field gel electrophoresis (PFGE). Antimicrobial resistance patterns of E. coli isolates were determined by using disc diffusion assay, and minimum inhibitory concentration of ciprofloxacin was determined by E-test. Plasmid-mediated quinolone resistance (PMQR) and extended spectrum beta-lactamase (ESBL) resistance genes were also screened through polymerase chain reactions. Sixty chicken meat samples were collected from different supermarkets and butchers in Sivas, Turkey. CR E. coli strains were determined in 59 (98.3%) chicken meat samples. By analyzing PFGE fingerprint data, 34 different pulsotypes were determined. All E. coli strains were found to be resistant to nalidixic acid, enrofloxacin, and norfloxacin. In addition, isolates were resistant to levofloxacin (40.7%), ampicillin (94.9%), trimethoprim-sulfamethoxazole (76.3%), tetracycline (69.5%), and chloramphenicol (44.1%). However, isolates were susceptible to imipenem and colistin. In this study, 81.4% of CR E. coli isolates were observed to have a multidrug-resistant profile, which is defined as resistance to three or more classes of antibiotics. Through phenotypic confirmation tests, five isolates (8.3%) were determined to be ESBL-producing. The PMQR genes were not determined in any of the isolates. Two isolates (3.4%) possessed the blaCTX-M and blaCMY-2 genes, and 40 isolates (67.8%) had the blaTEM gene. Taken together, retail raw chicken meat is highly contaminated with CR E. coli. However, these isolates are not found to be carriers of the PMQR genes, indicating a low public health problem.

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Prevalence of Escherichia coli in chicken carcasses from Mansoura, Egypt

Mansoura Veterinary Medical Journal ◽

10.35943/mvmj.2019.01.109 ◽

2019 ◽

Vol 20 (1) ◽

pp. 41-44

Author(s):

E Abo El-magd

Keyword(s):

Public Health ◽

Escherichia Coli ◽

Incidence Rate ◽

Clinical Relevance ◽

Chicken Meat ◽

E Coli ◽

Hygienic Measures ◽

Foodborne Infection

Objective: This study ended to detect the prevalence and public health important of Escherichia coli in chicken carcasses in Mansoura City. Procedures: A total of 100 whole frozen chicken carcasses purchased from supermarkets distributed at Mansoura city were examined to detect Escherichia coli. Results: Out of the 100 examined carcasses, 98 chicken carcasses were contaminated with Escherichia coli with an incidence rate 98%. Conclusion and clinical relevance: This study revealed that chicken meat are highly contaminated with E. coli and play an important role in transmission of E. coli to human, therefore special precautions and hygienic measures must be applied in abattoir during evisceration and dressing of carcasses, and in-home during handling and cooking of chickens to protect consumers from E. coli foodborne infection.

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Prevalence and Characterization of ESBL/AmpC Producing Escherichia coli from Fresh Meat in Portugal

Antibiotics ◽

10.3390/antibiotics10111333 ◽

2021 ◽

Vol 10 (11) ◽

pp. 1333

Author(s):

Lurdes Clemente ◽

Célia Leão ◽

Laura Moura ◽

Teresa Albuquerque ◽

Ana Amaro

Keyword(s):

Escherichia Coli ◽

Acquired Resistance ◽

Multidrug Resistant ◽

Resistance Mechanisms ◽

Selective Medium ◽

Poultry Meat ◽

Broiler Meat ◽

E Coli ◽

Antimicrobial Resistance Genes ◽

Meat Samples

The present study aimed to characterize the extended-spectrum β-lactamases and plasmid-mediated AmpC β-lactamases (ESBL/PMAβ) among Escherichia coli producers isolated from beef, pork, and poultry meat collected at retail, in Portugal. A total of 638 meat samples were collected and inoculated on selective medium for the search of E. coli resistant to 3rd generation cephalosporins. Isolates were characterized by antimicrobial susceptibility testing, molecular assays targeting ESBL/AmpC, plasmid-mediated quinolone resistance (PMQR), and plasmid-mediated colistin resistance (PMCR) encoding genes. The highest frequency of E. coli non-wild type to 3rd generation cephalosporins and fluoroquinolones was observed in broiler meat (30.3% and 93.3%, respectively). Overall, a diversity of acquired resistance mechanisms, were detected: blaESBL [blaCTX-M-1 (n = 19), blaCTX-M-15 (n = 4), blaCTX-M-32 (n = 12), blaCTX-M-55 (n = 8), blaCTX-M-65 (n = 4), blaCTX-M-27 (n = 2), blaCTX-M-9 (n = 1), blaCTX-M-14 (n = 11), blaSHV-12 (n = 27), blaTEM-52 (n = 1)], blaPMAβ [blaCMY-2 (n = 8)], PMQR [qnrB (n = 27), qnrS (n = 21) and aac(6’)-Ib-type (n = 4)] and PMCR [mcr-1 (n = 8)]. Our study highlights that consumers may be exposed through the food chain to multidrug-resistant E. coli carrying diverse plasmid-mediated antimicrobial resistance genes, posing a great hazard to food safety and a public health risk.

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Quantitative Analysis of Colistin-Resistant Escherichia coli in Retail Meat from Local Vietnamese Markets

BioMed Research International ◽

10.1155/2021/6678901 ◽

2021 ◽

Vol 2021 ◽

pp. 1-6

Author(s):

Thang N. Nguyen ◽

Diep T. Khong ◽

Ha V. Le ◽

Hoa T. Tran ◽

Quang N. Phan ◽

...

Keyword(s):

Escherichia Coli ◽

Food Contamination ◽

Chicken Meat ◽

Resistant Bacteria ◽

Meat Sample ◽

E Coli ◽

Drug Resistant Bacteria ◽

The Status ◽

Retail Meat ◽

Meat Samples

The spread of drug-resistant bacteria via food has contributed to the dissemination of resistant bacteria among humans. However, the status of food contamination with resistant bacteria, particularly the quantitative level of resistant bacteria in food, has not yet been well elucidated. In this study, the abundance of colistin-resistant Escherichia coli in meat samples was quantified to understand the origin of the contamination of meat available in local Vietnamese markets. Fifteen samples each of chicken and pork meat purchased from local Vietnamese markets were assessed for the presence of colistin-resistant E. coli with the mobile colistin resistance gene, mcr. The results showed that 40% (6/15) and 66% (10/15) of the pork and chicken meat samples, respectively, were contaminated with colistin-resistant E. coli. The median quantitative levels of colistin-resistant E. coli in the contaminated pork and chicken samples were 1.8 × 10 4 and 4.2 × 10 3 CFU/g, respectively. The results of phylogenetic analysis of isolates from a chicken meat sample showed that the contaminated colistin-resistant E. coli was a mix of multiple phylogenetical clones of bacteria that may have multiplied during sale. This is the first study to quantify the abundance of colistin-resistant E. coli in meat samples.

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Resistensi Antibiotik pada Escherichia coli yang Diisolasi dari Daging Ayam pada Pasar Tradisional di Kota Bogor (ANTIBIOTIC RESISTANCE IN ESCHERICHIA COLI ISOLATED FROM CHICKEN MEAT OF TRADITIONAL MARKETS IN THE CITY OF BOGOR)

jurnal veteriner ◽

10.19087/jveteriner.2019.20.1.125 ◽

2019 ◽

Vol 20 (1) ◽

pp. 125

Author(s):

Connie Januari ◽

Mirnawati Bachrum Sudarwanto ◽

Trioso Purnawarman

Keyword(s):

Escherichia Coli ◽

Antibiotic Resistance ◽

Bacterial Resistance ◽

Antibiotic Use ◽

Multidrug Resistant ◽

Chicken Meat ◽

Diffusion Method ◽

Negative Effects ◽

Disk Diffusion Method ◽

E Coli

Antibiotic use in farm is spread widely to treat of poultry disease including therapy, supportive or preventive use and as afeed additive to improve chicken performance. The negative effects of antibiotic use can increase the level of bacterial resistance to antibiotics. This study aimed to investigate on antibiotic resistance in Escherichia coli isolated from chicken meat that were sold in Traditional Market of Bogor City. A total of 175 samples of chicken meat were taken by purposive sampling method, out of 175 found 50 positive samples of E. coli. The samples were subjected to E. coli examination and the isolated E. coli were tested for the antibiotic resistance using eight antibiotics, i.e., amoxicillin, cefotaxime, colistin, nalidixid acid, streptomycin, erythromycin, oxytetracillin, and tetracycline. The study was conducted by using the disk diffusion method on Muller-Hinton agar according to the Clinical and Laboratory Standards Institute guidelines. The study showed E. coli isolated from chicken meat were resistance towards amoxicilin (90%), colistin (94%), nalidixid acid (86%), streptomycin (98%), erythromycin (98%), oxytetracillin (84%), tetracycline (86%), and cefotaxime antibiotics (12%). The proportion of multidrugresistant was 99%. The higher of multidrug-resistant indicated the E. coli would be a threat to public and environmental health.

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