scholarly journals Cyniclomyces guttulatus is an opportunistic pathogen in rabbits with coccidiosis

2019 ◽  
Author(s):  
Tuanyuan Shi ◽  
Hongchao Sun ◽  
Yuan Fu ◽  
Hao Lili ◽  
Yongxue Zhou ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Gastrointestinal Tract ◽  
Digestive Tract ◽  
Body Weight Gain ◽  
Clinical Signs ◽  
Opportunistic Pathogen ◽  
Culture Conditions ◽  
Severe Diarrhea ◽  
Positive Rate

AbstractCyniclomyces guttulatus is a common inhabitant of the gastrointestinal tract in rabbits, and large numbers are often present in feces of diarrheic rabbits. However, its relation with rabbit diarrhea has not been clearly identified. We isolated a C. guttulatus Zhejiang strain from a rabbit with severe diarrhea and optimized the culture conditions in YPG medium. The sequenced 18S and 26S ribosomal DNA fragments were 1559bp and 632bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from the dog and 100% homology with the 26S ribosomal sequence of the DPA-CGR1 and CGDPA-GP1 isolates from the rabbit and guinea pig. Our isolate was not pathogenic to healthy SPF rabbits. Instead, rabbits inoculated with the yeast had a slightly better body weight gain and higher food intake. Rabbits co-inoculated with C. guttulatus and the coccidian, E. intestinalis developed more severe coccidiosis as shown by clinical signs, and decreased body weight gain, diarrhea and death, associated with significantly higher fecal output of C. guttulatus vegetative cells but lower coccidian oocysts output than the rabbits inoculated with C. guttulatus or E. intestinalis alone. We also surveyed the prevalence of C. guttulatus in rabbits and found a positive rate of 83% in Zhejiang province. Our results indicate that C. guttulatus alone is not pathogenic to healthy rabbits, but could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.Author summaryCyniclomyces guttulatus, a commensal yeast in rabbit gastrointestinal tract, is very commonly seen in diarrhea cases. However, it is unclear whether it causes or is a co-cause of diarrhea with other pathogens. Here, a C. guttulatus Zhejiang strain was firstly isolated from a rabbit with severe diarrhea and the culture conditions in YPG medium were optimized. Then, it was identified in morphology and molecular. It was agreed with the previous description in morphology and showed a closer phylogenetic relationship with other strains originated from herbivores than those from the carnivore. Finally, the C. guttulatus Zhejiang strain was inoculated to SPF rabbits singly or co-inoculated with Eimeria intestinalis. All of the results in animal assays show C. guttulatus alone is not pathogenic but seems a probiotic microorganism in rabbits. However, it could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.

scholarly journals Cyniclomyces guttulatus is an opportunistic pathogen in rabbits with coccidiosis

2019 ◽  
Author(s):  
Tuanyuan Shi ◽  
Hongchao Sun ◽  
Yuan Fu ◽  
Lili Hao ◽  
Yongxue Zhou ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Clinical Signs ◽  
Opportunistic Pathogen ◽  
Culture Conditions ◽  
Fecal Output ◽  
Large Numbers ◽  
Positive Rate ◽  
Probiotic Microorganism

Abstract Background: Cyniclomyces guttulatus is a common inhabitant of the gastrointestinal tract in rabbits, and large numbers are often present in feces of diarrheic rabbits. However, its relation with rabbit diarrhea has not been clearly identified. Relationship between C. guttulatus and the rabbit are identified in this paper.Methods: YPG (pH 1.5) medium and 96-well culture plate were used to isolate C. guttulatus from rabbit. Automatic microbial growth curve analyser and optical density scanner were used to optimized culture conditions of the C. guttulatus strain in the YPG medium. Microscope observation, PCR and gene sequencing were used to identify the C. guttulatus strain. Animal inoculation with the C. guttulatus strain or co-inoculation with E. intestinalis were used identify relationship between C. guttulatus and the rabbit. Results: A C. guttulatus Zhejiang strain was isolated from a rabbit with diarrhea and the culture conditions in YPG medium were optimized. The sequenced 18S and 26S ribosomal DNA fragments were 1559bp and 632bp, respectively, and showed 99.8% homology with the 18S ribosomal sequence of the NRRL Y-17561 isolate from the dog and 100% homology with the 26S ribosomal sequence of the DPA-CGR1 and CGDPA-GP1 isolates from the rabbit and guinea pig. Our isolate was not pathogenic to healthy SPF rabbits. Instead, rabbits inoculated with the yeast had a slightly better body weight gain and higher food intake. Rabbits co-inoculated with C. guttulatus and the coccidian, E. intestinalis developed more severe coccidiosis as shown by clinical signs, and decreased body weight gain, diarrhea and death, associated with significantly higher fecal output of C. guttulatus vegetative cells but lower coccidian oocysts output than the rabbits inoculated with C. guttulatus or E. intestinalis alone. We also surveyed the prevalence of C. guttulatus in rabbits and found a positive rate of 83% in Zhejiang province. Conclusions: Our results indicate that C. guttulatus alone is not pathogenic to healthy rabbits and seems a probiotic microorganism in rabbits, but could become an opportunistic pathogen when the digestive tract is damaged by other pathogens such as coccidia.

scholarly journals Feeding microparticle protein diet combined with Lactobacillus sp. on existence of intestinal bacteria and growth of broiler chickens

2018 ◽  
Vol 43 (3) ◽  
pp. 265 ◽  
Author(s):  
M. A. Cholis ◽  
N. Suthama ◽  
B. Sukamto
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Digestive Tract ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Dietary Treatment ◽  
Protein Diet ◽  
Carcass Weight ◽  
Intact Protein ◽  
Daily Body Weight

The objective of the study was to evaluate feeding effect of microparticle protein derived from soybean meal and fish meal combined with Lactobacillus sp. at 1.2 mL on physiological condition of digestive tract and productivity of broiler. Total of 192 broiler, with initial body weight was 481.0 ± 67.2 g, given dietary treatment starting on day 21 until day 42. The experiment was assigned in a completely randomized design with 8 treatments and 4 replications. Dietary treatment were 21% intact protein (T0), 18% intact protein (T1), 21% microparticle protein (T2), 18% microparticle protein (T3), T0+Lactobacillus sp.1.2 mL (T4), T1+Lactobacillus sp.1.2 mL (T5), T2+Lactobacillus sp.1.2 mL (T6), T3+Lactobacillus sp.1.2 mL (T7). Parameters measured were total ileal lactic acid bacteria (LAB), Coliform and pH, rate of passage, daily body weight gain (DWG) and also carcass weight. Data were subjected to analysis of variance and followed by Duncan multiple range test (P<0.05). Experimental results showed that microparticle protein diet added with Lactobacillus sp. (P<0.05) increased total LAB and DWG, but decreased Coliform population, and slowed down the rate of passage. However, carcass weight was not significantly affected by treatments. Feeding microparticle protein (18%) with addition of Lactobacillus sp. (1.2 mL) can improve to be better condition of digestive tract based on higher LAB and lower Coliform populations, and increase daily body weight gain with the same carcass weight. 

Experimental inoculation of day-old ducks with Brachyspira pilosicoli and B. alvinipulli

2011 ◽  
Vol 59 (2) ◽  
pp. 165-174 ◽  
Author(s):  
Ákos Thuma ◽  
Ádám Dán ◽  
Éva Kaszanyitzky ◽  
Béla Fazekas ◽  
Ádám Tóth ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Mucous Membrane ◽  
Body Weight Gain ◽  
Clinical Signs ◽  
Experimental Period ◽  
The Body ◽  
Bursa Of Fabricius ◽  
Brachyspira Pilosicoli ◽  
Significant Difference

Two groups of one-day-old Peking ducklings (Groups I and II, 12 birds/group) were inoculated orally withBrachyspira pilosicoliand two groups withB. alvinipulli(Groups III and IV, 12 birds/group). T-2 toxin was added to the feed of Groups II and IV in a dose of 1 mg/kg of feed. Groups V and VI served as uninfected control groups (ducks of Group VI received T-2 toxin). The body weight gain of the ducks was measured and clinical signs were monitored continuously. The birds were sacrificed and necropsied on days 7, 14, 21, and 28 post infection (PI). The liver, spleen, kidney, thymus, bursa of Fabricius, ileum, caecum and colon were examined histologically. Culturing ofBrachyspiraspp. and immunohistochemistry were performed from the sampled parts of the intestines as well. No gross pathological or histological lesions that could be associated withB. pilosicoliorB. alvinipulliwere detectable in the intestinal mucous membrane including the colonised intestinal glands. Mortality did not occur during the experimental period. Decrease in body weight gain was significant in the T-2-toxin-treated groups, and it was slight (not significant) in theBrachyspira-infected groups. Crust on the beaks, necrosis, crusting and ulceration in the mucous membrane of the oral cavity and on the skin of the feet, atrophy of the thymus and bursa of Fabricius due to the effect of T-2 toxin, accompanied by lymphocyte depletion, were observed. These lesions were most prominent on days 14 and 21 PI but were seen on day 28 PI as well. Immunohistochemical detection and reisolation ofB. pilosicoliandB. alvinipulliwere successful on days 7, 14, 21 and 28 days from different segments of the intestine of certain birds, but no significant difference was observed in the colonisation rate between the T-2-toxin-treated and the untreated groups.

Tamarind Seed Polysaccharide

2013 ◽  
Vol 32 (3) ◽  
pp. 198-208 ◽  
Author(s):  
James T. Heimbach ◽  
Hiroshi Egawa ◽  
Palma Ann Marone ◽  
Mark R. Bauter ◽  
Elke Kennepohl
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Food Consumption ◽  
Body Weight Gain ◽  
Clinical Chemistry ◽  
Clinical Signs ◽  
Female Rats ◽  
Feed Consumption ◽  
Test Substance ◽  
Tamarind Seed

Forty male and 40 female Crl:SD® CD® IGS rats were fed diets containing 0, 40 000, 80 000, or 120 000 ppm tamarind seed polysaccharide (equivalent to 3450.8, 6738.9, or 10 597.1 mg/kg bw/day and 3602.1, 7190.1, or 10 690.7 mg/kg bw/day for males and females, respectively) for 28 days. Animals were observed for adverse clinical signs, body weight, feed consumption, hematology and clinical chemistry parameters, urinalysis values were recorded, and at the end of the study the rats underwent a full necropsy. Functional Observational Battery (FOB) and Motor Activity (MA) tests were performed on all animals. There were no mortalities, no clinical or ophthalmologic signs, body weight, body weight gain, food consumption and food efficiency, FOB or MA findings associated with the administration of tamarind seed polysaccharide. Initial statistically significant decreases in body weight gain and food consumption resolved after the first week and were considered the result of reduced palatability. There were no adverse changes in hematology, coagulation, clinical chemistry or urinalysis parameters in male or female rats considered the result of test substance administration. At necropsy, there were no macroscopic, histopathological findings, estrus cycle, or organ weight changes deemed related to administration of the test substance. Under the conditions of this study and based on the toxicological endpoints evaluated, the no-observed-adverse-effect level (NOAEL) for tamarind seed polysaccharide in the diet was the highest concentration tested of 120 000 ppm (equivalent to 10 597 mg/kg bw/day and 10 691 mg/kg bw/day for male and female rats, respectively).

scholarly journals Secretory Defense Response in the Bird’s Gastro-Intestinal Tract and Nutritional Strategies to Modulate It

2021 ◽  
Author(s):  
Luis-Miguel Gomez-Osorio ◽  
Zhengyu Jiang ◽  
Qian Zhang ◽  
Hui Yan ◽  
Ana-Maria Villegas ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Immune Response ◽  
Body Weight ◽  
Weight Gain ◽  
Gastrointestinal Tract ◽  
Defense Response ◽  
Body Weight Gain ◽  
Nutritional Factors ◽  
Gastro Intestinal Tract ◽  
Nutritional Strategies

The Gastrointestinal tract (GIT) is a very complex environment which converges a lot of players including nutrients, microorganisms, pathogens, cells, and peptides which determine the type of outcome against threats affecting feed efficiency and body weight gain. Traditionally, GIT is examined as a selective barrier which permit or deny the entrance of molecules, but beyond of that, it is a crucial place to produce important proteins for the host which, at least in part, determine the outcome for a threat such as microorganisms, toxins, anti-nutritional factors, among others. During the non-antibiotic promotants time, there is a necessity to understand how this system works and how we can modulate through nutrition, in part to take advantage of this, and support a better immune response and nutrient absorption in challenged poultry environments. The goal of this chapter is to review the different mechanism of immunity in the GIT emphasizing on secretory defense response and the nutritional strategies including fiber and fatty acids to improve it.

scholarly journals Single Oral Acute Toxicity of Banhasasim-Tang and Its Antiobesity Effect on Diet-Induced Obese Mice and 3T3-L1 Adipocytes

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Sae-Rom Yoo ◽  
Soo-Jin Jeong ◽  
Mee-young Lee ◽  
Hyeun-Kyoo Shin ◽  
Chang-Seob Seo ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Acute Toxicity ◽  
Body Weight Gain ◽  
Clinical Signs ◽  
The Body ◽  
Dependent Manner ◽  
Obese Mice ◽  
Triglyceride Accumulation ◽  
Sd Rats

We had tested antiobesity effect of 52 traditional herbal formulas in 3T3-L1 adipocyte, and Banhasasim-tang (BHSST) was chosen as one of the effective medications to inhibit triglyceride accumulation. We investigated the antiobesity effect of BHSST on 3T3-L1 adipocytes and high-fat diet- (HFD-) induced obese mice. In addition, we evaluated the acute toxicity of BHSST in Sprague Dawley (SD) rats. Differentiated 3T3-L1 cells were treated with various concentrations of BHSST for 8 days. Accumulated triglyceride level and the expressions of adipogenesis-related genes and proteins were subsequently investigated. To evaluate the single oral toxicity of BHSST, the SD rats of each sex were administered a single dose (5000 mg/kg) of BHSST via oral gavage; the control group received vehicle only. After a single administration, the mortality, clinical signs, gross findings, and body weight were monitored for 15 days. Male C57BL/6J mice were fed HFD for 4 weeks to induce obesity and randomly received 50 mg/kg of Orlistat (n=12, OR), 200 mg/kg of BHSST (n=12, B200), and 1000 mg/kg of BHSST (n=12, B1000) for another 8 weeks. BHSST suppressed the triglyceride contents and lipid accumulation in a dose-dependent manner in 3T3-L1 adipocytes. BHSST also downregulated the adipogenesis-related gene levels and protein expression compared with those in undifferentiated adipocytes. In a single oral dose toxicity study, there was no adverse effect on mortality, clinical signs, body weight changes, and gross findings in the treatment group. HFD-fed mice treated with BHSST showed significantly reduced body weight gain, food efficiency ratio, and white adipose tissue weight. The medial lethal dose (LD50) of BHSST was 5000 mg/kg/day body weight for each sex in the rats. BHSST decreased the body weight gain in HFD-fed obese mice and inhibited triglyceride accumulation via a cascade of multiple factors at the mRNA and protein levels in 3T3-L1 adipocytes.

scholarly journals Isolat Bakteri Asam Laktat Sebagai Probiotik dengan Vaksinasi AI dan ND dalam Pembentukan Titer Antibodi Dan Bobot Badan Ayam Jantan Tipe Medium

2017 ◽  
Vol 14 (2) ◽  
Author(s):  
Rudy Sutrisna
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Health Status ◽  
Digestive Tract ◽  
Body Weight Gain ◽  
The Body ◽  
Water Medium ◽  
Average Value ◽  
Antibody Titers ◽  
Medium Type

Isolates of Lactic Acid Bacteria isolates assembled into a mixture of LAB as probiotics . LAB probiotic drops introduced through the mouth with the aim of suppressing pathogens and is expected to improve the health status of rooster type of medium . Isolates in the digestive tract will undergo adaptation by temperature , acidity and pH changes in the gut . The health status of the chicken can be expressed as growth to form the body weight , and the growth of LAB . In addition , AI and ND vaccination in healthy chickens conditions will produce antibodies to both . The success of vaccination can be evaluated from the results of the formation of chicken blood serum titre of research . Analysis of descriptive data on the dynamics of the growth of LAB , the formation of antibody titer and body weight gain 24-31 day old chickens . Based on the results of this study revealed that isolates of LAB can be used simultaneously in bercoccidiostat feed . LAB isolates may develop in the digestive tract rooster medium type as probiotics. The dynamics of the growth of a population produces a mixture of isolates of LAB isolates (mixture of B1, B3, B4, B5 as probiotics) in the highest reaches of the ilium and jejunum 14.00 x 10-6 13.5 x 10-6 CFU/ml. Test isolates viability of probiotic candidates in all isolates produced distilled water medium can live up to 72 hours of incubation . In addition, there is a synergy results in the formation of antibody titers ND uniform T3 treatment. T3 treatment is given probiotics 2x chicken every week for 3 weeks . In AI vaccination resulted in antibody titers are not uniform. Body weight gain reached the age of 24-31 days in a row 75.83 g, 76.54 g, 81.58 g, 87.04 g. Weight age of 31 days the average value obtained successively from the highest 329.16 g, 328.54 g, 325.54 g and 324.08 g lowest . Keywords; isolates LAB, probiotics, antibody titers, rooster layer medium type.

scholarly journals Effects of exogenous β-glucanase on ileal digesta soluble β-glucan molecular weight, digestive tract characteristics, and performance of coccidiosis vaccinated broiler chickens fed hulless barley-based diets with and without medication

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0236231
Author(s):  
Namalika D. Karunaratne ◽  
Rex W. Newkirk ◽  
Nancy P. Ames ◽  
Andrew G. Van Kessel ◽  
Michael R. Bedford ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Digestive Tract ◽  
Feed Efficiency ◽  
Body Weight Gain ◽  
Partial Replacement ◽  
Hulless Barley ◽  
Beta Glucanase

Introduction Limited use of medication in poultry feed led to the investigation of exogenous enzymes as antibiotic alternatives for controlling enteric disease. The objective of this study was to evaluate the effects of diet β-glucanase (BGase) and medication on β-glucan depolymerization, digestive tract characteristics, and growth performance of broilers. Materials and methods Broilers were fed hulless barley (HB) based diets with BGase (Econase GT 200P from AB Vista; 0 and 0.1%) and medication (Bacitracin and Salinomycin Na; with and without) arranged as a 2 × 2 factorial. In Experiment 1, 160 broilers were housed in cages from d 0 to 28. Each treatment was assigned to 10 cages. In Experiment 2, broilers (2376) were housed in floor pens and vaccinated for coccidiosis on d 5. Each treatment was assigned to one floor pen in each of nine rooms. Results In Experiment 1, the soluble β-glucan weighted average molecular weight (Mw) in the ileal digesta was lower with medication in the 0% BGase treatments. Peak molecular weight (Mp) and Mw were lower with BGase regardless of medication. The maximum molecular weight for the smallest 10% β-glucan (MW-10%) was lower with BGase addition. In Experiment 2, Mp was lower with medication in 0% BGase treatments. Beta-glucanase resulted in lower Mp regardless of medication, and the degree of response was lower with medication. The MW-10% was lower with BGase despite antibiotic addition. Body weight gain and feed efficiency were higher with medication regardless of BGase use through-out the trial (except d 11–22 feed efficiency). Beta-glucanase resulted in higher body weight gain after d 11 and worsened and improved feed efficiency before and after d 11, respectively, in unmedicated treatments. Conclusion BGase and medication caused the depolymerization of soluble ileal β-glucan. Beta-glucanase acted as a partial replacement for diet medication by increasing growth performance in coccidiosis vaccinated broilers.

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