The Impact of Zeta Potential and Physicochemical Properties of TiO2-Based Nanocomposites on Their Biological Activity

Nanomaterials, such as hydroxyapatite nanoparticles show a great promise for medical applications due to their unique properties at the nanoscale. However, there are concerns about the safety of using these materials in biological environments. Despite a great number of published studies of nanoobjects and their aggregates or agglomerates, the impact of their physicochemical properties (such as particle size, surface area, purity, details of structure and degree of agglomeration) on living cells is not yet fully understood. Significant differences in these properties, resulting from different manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B and J774.1 to assess the influence of the nanoparticles on immune, reproductive and respiratory systems.

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Assessment of the impact of manufacturing changes on the physicochemical properties and biological activity of Her1-ECD vaccine during product development

Vaccine ◽

10.1016/j.vaccine.2015.05.018 ◽

2015 ◽

Vol 33 (35) ◽

pp. 4292-4299 ◽

Cited By ~ 3

Author(s):

Katia Garcia Duardo ◽

Yadira Prieto Curbelo ◽

Judith Raymond Pous ◽

Estela Yamilet Rabasa Legón ◽

Belinda Sánchez Ramírez ◽

...

Keyword(s):

Biological Activity ◽

Product Development ◽

Physicochemical Properties ◽

The Impact

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Demonstration of Equivalence of Generic Glatiramer Acetate and Copaxone®

Frontiers in Pharmacology ◽

10.3389/fphar.2021.760726 ◽

2021 ◽

Vol 12 ◽

Author(s):

Peter Lipsky ◽

Patrick T. Vallano ◽

Jeffrey Smith ◽

Walter Owens ◽

Daniel Snider ◽

...

Keyword(s):

Biological Activity ◽

Amino Acid ◽

Physicochemical Properties ◽

Glatiramer Acetate ◽

Drug Administration ◽

Food And Drug Administration ◽

Reaction Scheme ◽

Amino Acid Sequences ◽

The Us ◽

The Impact

The objective of the current work was to demonstrate the equivalence of Mylan’s glatiramer acetate (GA) to that of the reference product Copaxone® (COP) using the four criteria for active pharmaceutical ingredient sameness as established by the US Food and Drug Administration (FDA). The reaction scheme used to produce Mylan’s glatiramer acetate (MGA) was compared with that of COP, determined from publicly available literature. Comparative analyses of MGA and COP were performed for physicochemical properties such as amino acid composition and molecular weight distributions. Spectroscopic fingerprints were obtained using circular dichroism spectroscopy. Structural signatures for polymerization and depolymerization including total diethylamine (DEA) content, relative proportions of DEA-adducted amino acids, and N-and C-terminal amino acid sequences were probed with an array of highly sensitive analytical methods. Biological activity of the products was assessed using validated murine Experimental autoimmune encephalomyelitis (EAE) models of multiple sclerosis. MGA is produced using the same fundamental reaction scheme as COP and was shown to have equivalent physicochemical properties and composition. Analyses of multiple structural signatures demonstrated equivalence of MGA and COP with regard to polymerization, depolymerization, and propagational shift. Examination of the impact on prevention and treatment of EAE demonstrated equivalence of MGA and COP with respect to both activity and toxicity, and thereby provided confirmatory evidence of sameness. A rigorous, multi-pronged comparison of MGA and COP produced using an equivalent fundamental reaction scheme demonstrated equivalent physicochemical properties, structural signatures for polymerization and depolymerization, and biological activity as evidenced by comparable effects in EAE. These studies demonstrate the equivalence of MGA and COP, establishing active ingredient sameness by the US Food and Drug Administration (FDA) criteria for GA, and provide compelling evidence that the FDA-approved generic MGA can be substituted for COP for the treatment of patients with relapsing-remitting MS.

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Synthesis and Biological Activity of Brassinosteroid Analogues with a Nitrogen-Containing Side Chain

International Journal of Molecular Sciences ◽

10.3390/ijms22010155 ◽

2020 ◽

Vol 22 (1) ◽

pp. 155

Author(s):

Mikhail V. Diachkov ◽

Karoll Ferrer ◽

Jana Oklestkova ◽

Lucie Rarova ◽

Vaclav Bazgier ◽

...

Keyword(s):

Biological Activity ◽

Functional Groups ◽

Side Chain ◽

Biotic And Abiotic Stresses ◽

Short Side ◽

Arabidopsis Root ◽

Physiological Processes ◽

Growth Promoting ◽

The Impact ◽

Brassinosteroid Analogues

Brassinosteroids are a class of plant hormones that regulate a broad range of physiological processes such as plant growth, development and immunity, including the suppression of biotic and abiotic stresses. In this paper, we report the synthesis of new brassinosteroid analogues with a nitrogen-containing side chain and their biological activity on Arabidopis thaliana. Based on molecular docking experiments, two groups of brassinosteroid analogues were prepared with short and long side chains in order to study the impact of side chain length on plants. The derivatives with a short side chain were prepared with amide, amine and ammonium functional groups. The derivatives with a long side chain were synthesized using amide and ammonium functional groups. A total of 25 new brassinosteroid analogues were prepared. All 25 compounds were tested in an Arabidopsis root sensitivity bioassay and cytotoxicity screening. The synthesized substances showed no significant inhibitory activity compared to natural 24-epibrassinolide. In contrast, in low concentration, several compounds (8a, 8b, 8e, 16e, 22a and 22e) showed interesting growth-promoting activity. The cytotoxicity assay showed no toxicity of the prepared compounds on cancer and normal cell lines.

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Combinations of Freeze-Dried Amorphous Vardenafil Hydrochloride with Saccharides as A Way to Enhance Dissolution Rate and Permeability

Pharmaceuticals ◽

10.3390/ph14050453 ◽

2021 ◽

Vol 14 (5) ◽

pp. 453

Author(s):

Gabriela Wiergowska ◽

Dominika Ludowicz ◽

Kamil Wdowiak ◽

Andrzej Miklaszewski ◽

Kornelia Lewandowska ◽

...

Keyword(s):

Physicochemical Properties ◽

Density Functional ◽

Hydroxypropyl Methylcellulose ◽

Fold Increase ◽

Crystalline Form ◽

Amorphous Form ◽

Freeze Dried ◽

Apparent Solubility ◽

Gastrointestinal Epithelium ◽

The Impact

To improve physicochemical properties of vardenafil hydrochloride (VAR), its amorphous form and combinations with excipients—hydroxypropyl methylcellulose (HPMC) and β-cyclodextrin (β-CD)—were prepared. The impact of the modification on physicochemical properties was estimated by comparing amorphous mixtures of VAR to their crystalline form. The amorphous form of VAR was obtained as a result of the freeze-drying process. Confirmation of the identity of the amorphous dispersion of VAR was obtained through the use of comprehensive analysis techniques—X-ray powder diffraction (PXRD) and differential scanning calorimetry (DSC), supported by FT-IR (Fourier-transform infrared spectroscopy) coupled with density functional theory (DFT) calculations. The amorphous mixtures of VAR increased its apparent solubility compared to the crystalline form. Moreover, a nearly 1.3-fold increase of amorphous VAR permeability through membranes simulating gastrointestinal epithelium as a consequence of the changes of apparent solubility (Papp crystalline VAR = 6.83 × 10−6 cm/s vs. Papp amorphous VAR = 8.75 × 10−6 cm/s) was observed, especially for its combinations with β-CD in the ratio of 1:5—more than 1.5-fold increase (Papp amorphous VAR = 8.75 × 10−6 cm/s vs. Papp amorphous VAR:β-CD 1:5 = 13.43 × 10−6 cm/s). The stability of the amorphous VAR was confirmed for 7 months. The HPMC and β-CD are effective modifiers of its apparent solubility and permeation through membranes simulating gastrointestinal epithelium, suggesting a possibility of a stronger pharmacological effect.

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Ethanol/Gasoline Blends as Alternative Fuel in Last Generation Spark-Ignition Engines: A Review on CO and HC Engine Out Emissions

Energies ◽

10.3390/en14134034 ◽

2021 ◽

Vol 14 (13) ◽

pp. 4034

Author(s):

Paolo Iodice ◽

Massimo Cardone

Keyword(s):

Physicochemical Properties ◽

Alternative Fuels ◽

Experimental Studies ◽

Alternative Fuel ◽

Exhaust Emissions ◽

Spark Ignition ◽

Operating Conditions ◽

Spark Ignition Engine ◽

Spark Ignition Engines ◽

The Impact

Among the alternative fuels existing for spark-ignition engines, ethanol is considered worldwide as an important renewable fuel when mixed with pure gasoline because of its favorable physicochemical properties. An in-depth and updated investigation on the issue of CO and HC engine out emissions related to use of ethanol/gasoline fuels in spark-ignition engines is therefore necessary. Starting from our experimental studies on engine out emissions of a last generation spark-ignition engine fueled with ethanol/gasoline fuels, the aim of this new investigation is to offer a complete literature review on the present state of ethanol combustion in last generation spark-ignition engines under real working conditions to clarify the possible change in CO and HC emissions. In the first section of this paper, a comparison between physicochemical properties of ethanol and gasoline is examined to assess the practicability of using ethanol as an alternative fuel for spark-ignition engines and to investigate the effect on engine out emissions and combustion efficiency. In the next section, this article focuses on the impact of ethanol/gasoline fuels on CO and HC formation. Many studies related to combustion characteristics and exhaust emissions in spark-ignition engines fueled with ethanol/gasoline fuels are thus discussed in detail. Most of these experimental investigations conclude that the addition of ethanol with gasoline fuel mixtures can really decrease the CO and HC exhaust emissions of last generation spark-ignition engines in several operating conditions.

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Silver Nanoparticles Stabilized with Phosphorus-Containing Heterocyclic Surfactants: Synthesis, Physico-Chemical Properties, and Biological Activity Determination

Nanomaterials ◽

10.3390/nano11081883 ◽

2021 ◽

Vol 11 (8) ◽

pp. 1883

Author(s):

Martin Pisárčik ◽

Miloš Lukáč ◽

Josef Jampílek ◽

František Bilka ◽

Andrea Bilková ◽

...

Keyword(s):

Silver Nanoparticles ◽

Biological Activity ◽

Zeta Potential ◽

Chain Length ◽

Alkyl Chain ◽

Chemical Properties ◽

Alkyl Chain Length ◽

Microbial Pathogens ◽

Physico Chemical ◽

Phosphorus Containing

Phosphorus-containing heterocyclic cationic surfactants alkyldimethylphenylphospholium bromides with the alkyl chain length 14 to 18 carbon atoms were used for the stabilization of silver nanodispersions. Zeta potential of silver nanodispersions ranges from +35 to +70 mV, which indicates the formation of stable silver nanoparticles (AgNPs). Long-chain heptadecyl and octadecyl homologs of the surfactants series provided the most intensive stabilizing effect to AgNPs, resulting in high positive zeta potential values and smaller diameter of AgNPs in the range 50–60 nm. A comparison with non-heterocyclic alkyltrimethylphosphonium surfactants of the same alkyl chain length showed better stability and more positive zeta potential values for silver nanodispersions stabilized with heterocyclic phospholium surfactants. Investigations of biological activity of phospholium-capped AgNPs are represented by the studies of antimicrobial activity and cytotoxicity. While cytotoxicity results revealed an increased level of HepG2 cell growth inhibition as compared with the cytotoxicity level of silver-free surfactant solutions, no enhanced antimicrobial action of phospholium-capped AgNPs against microbial pathogens was observed. The comparison of cytotoxicity of AgNPs stabilized with various non-heterocyclic ammonium and phosphonium surfactants shows that AgNPs capped with heterocyclic alkyldimethylphenylphospholium and non-heterocyclic triphenyl-substituted phosphonium surfactants have the highest cytotoxicity among silver nanodispersions stabilized by the series of ammonium and phosphonium surfactants.

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Comparison of Osmotic Resistance, Shape and Transmembrane Potential of Erythrocytes Collected from Healthy and Fed with High Fat-Carbohydrates Diet (HF-CD) Pigs—Protective Effect of Cistus incanus L. Extracts

Materials ◽

10.3390/ma14041050 ◽

2021 ◽

Vol 14 (4) ◽

pp. 1050

Author(s):

Sylwia Cyboran-Mikołajczyk ◽

Robert Pasławski ◽

Urszula Pasławska ◽

Kacper Nowak ◽

Michał Płóciennik ◽

...

Keyword(s):

Biological Activity ◽

Physical Properties ◽

Plasma Lipid ◽

Membrane Lipid ◽

Osmotic Resistance ◽

High Fat ◽

Atherosclerotic Lesions ◽

Cistus Incanus ◽

Osmotic Resistance Of Erythrocytes ◽

The Impact

Long-term high fat-carbohydrates diet (HF-CD) contributes to the formation of irreversible changes in the organism that lead to the emergence of civilization diseases. In this study, the impact of three-month high-fat diet on the physical properties of erythrocytes (RBCs) was studied. Furthermore, the biological activity of Cistus incanus L. extracts, plant known with high pro-health potential, in relation to normal and HF-CD RBCs, was determined. Obtained results have shown that, applied HF-CD modified shape, membrane potential and osmotic resistance of erythrocytes causing changes in membrane lipid composition and the distribution of lipids. The impact of HF-CD on physical properties of RBCs along with atherosclerotic lesions of the artery was visible, despite the lack of statistically significant changes in blood morphology and plasma lipid profile. This suggests that erythrocytes may be good markers of obesity-related diseases. The studies of biological activity of Cistus incanus L. extracts have demonstrated that they may ameliorate the effect of HF-CD on erythrocytes through the membrane-modifying and antioxidant activity.

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Synthetic auxin-based double salt ionic liquids as herbicides with improved physicochemical properties and biological activity

Journal of Molecular Liquids ◽

10.1016/j.molliq.2021.116452 ◽

2021 ◽

pp. 116452

Author(s):

Tomasz Rzemieniecki ◽

Marta Wojcieszak ◽

Katarzyna Materna ◽

Tadeusz Praczyk ◽

Juliusz Pernak

Keyword(s):

Biological Activity ◽

Ionic Liquids ◽

Physicochemical Properties ◽

Double Salt ◽

Synthetic Auxin

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Dye Separation and Antibacterial Activities of Polyaniline Thin Film-Coated Poly(phenyl sulfone) Membranes

Membranes ◽

10.3390/membranes11010025 ◽

2020 ◽

Vol 11 (1) ◽

pp. 25

Author(s):

Javed Alam ◽

Arun Kumar Shukla ◽

Mohammad Azam Ansari ◽

Fekri Abdulraqeb Ahmed Ali ◽

Mansour Alhoshan

Keyword(s):

Zeta Potential ◽

Surface Properties ◽

Antibacterial Activities ◽

Pani Film ◽

Force Microscopy ◽

Water Permeation ◽

Membrane Pores ◽

Membrane Performance ◽

Phenyl Sulfone ◽

The Impact

We fabricated a nanofiltration membrane consisting of a polyaniline (PANI) film on a polyphenylsulfone (PPSU) substrate membrane. The PANI film acted as a potent separation enhancer and antimicrobial coating. The membrane was analyzed via scanning electron microscopy and atomic force microscopy to examine its morphology, topography, contact angle, and zeta potential. We aimed to investigate the impact of the PANI film on the surface properties of the membrane. Membrane performance was then evaluated in terms of water permeation and rejection of methylene blue (MB), an organic dye. Coating the PPSU membrane with a PANI film imparted significant advantages, including finely tuned nanometer-scale membrane pores and tailored surface properties, including increased hydrophilicity and zeta potential. The PANI film also significantly enhanced separation of the MB dye. The PANI-coated membrane rejected over 90% of MB with little compromise in membrane permeability. The PANI film also enhanced the antimicrobial activity of the membrane. The bacteriostasis (BR) values of PANI-coated PPSU membranes after six and sixteen hours of incubation with Escherichia coli were 63.5% and 95.2%, respectively. The BR values of PANI-coated PPSU membranes after six and sixteen hours of incubation with Staphylococcus aureus were 70.6% and 88.0%, respectively.

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