Acylated Ghrelin and Circulatory Oxidative Stress Markers Responses to Acute Resistance and Aerobic Exercise in Postmenopausal Women

Background:Since exercise increases the production of reactive oxygen species in different tissues, the objective of this study is to evaluate, compare and correlate the acute effects of aerobic and resistance exercise in circulatory markers of oxidative stress and acylated ghrelin (AG) in postmenopausal women.Methods:Ten postmenopausal women completed different protocols: a control session (CON), an aerobic exercise session (AERO); and a single-set (SSR) or 3-set (MSR) resistance exercise protocol.Results:After exercise, both MSR (P = .06) and AERO (P = .02) sessions showed significant increased lipid peroxidation compared with baseline levels. CON and SSR sessions showed no differences after exercise. No differences were found between sessions at any time for total glutathione, glutathione dissulfide or AG concentrations.Conclusions:Exercise significantly increased lipid peroxidation compared with baseline values. As pro oxidant stimuli is necessary to promote chronic adaptations to the antioxidant defenses induced by exercise, our findings are important to consider when evaluating exercise programs prescription variables aiming quality of life in this population.

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Effects of Vitamin D and Simvastatin on Inflammatory and Oxidative Stress Markers of High-Fat Diet-Induced Obese Rats

Journal of Scientific Research in Medical and Biological Sciences ◽

10.47631/jsrmbs.v2i3.297 ◽

2021 ◽

Vol 2 (3) ◽

pp. 39-50

Author(s):

Abdel-Moniem A. Makhlouf ◽

Atef M. Mahmoud ◽

Rania G. Ibrahim ◽

Yasmeen S. Abdel Aziz

Keyword(s):

Oxidative Stress ◽

Vitamin D ◽

High Fat Diet ◽

Male Rats ◽

Antioxidant Defenses ◽

Control Group ◽

High Fat ◽

Stress Markers ◽

Obese Rats ◽

Markers Of Oxidative Stress

Purpose: This study was aimed to evaluate the antioxidant and anti-inflammatory effects of vitamin D and Simvastatin (SIM) on a high-fat diet (HFD) induced-obese rats. Methods: 40 adult male rats were divided into four groups: control group, HFD, HFD + vitamin D, and HFD + SIM for 14 weeks. Vitamin D or SIM supplementation was done for the last 6 weeks. Vitamin D dosage was 500 IU/kg, while SIM dosage was 10 mg/kg. Interleukin-6 (IL-6) concentration and markers of oxidative stress including malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and reduced glutathione(GSH) concentrations in serum were determined using ELISA kits and spectrophotometry methods, respectively. Results: Treatment with vitamin D or SIM could significantly reduce IL-6 and MDA and increases SOD, GPx activities, and GSH levels. Oxidative stress can result not only from increased ROS production but also from dysfunctional antioxidant defenses. Conclusion: From the experimental results, it was observed that SIM and vitamin D could attenuate oxidative stress and inflammation markers associated with obesity.

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Oxidative Damage in the Aging Heart: an Experimental Rat Model

The Open Cardiovascular Medicine Journal ◽

10.2174/1874192401509010078 ◽

2015 ◽

Vol 9 (1) ◽

pp. 78-82 ◽

Cited By ~ 2

Author(s):

Gustavo Lenci Marques ◽

Francisco Filipak Neto ◽

Ciro Alberto de Oliveira Ribeiro ◽

Samuel Liebel ◽

Rogério de Fraga ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Superoxide Dismutase ◽

Oxidative Damage ◽

Catalase Activity ◽

Protein Carbonylation ◽

Antioxidant Defenses ◽

Stress Markers ◽

Complex Process ◽

Protein Thiols

Introduction: Several theories have been proposed to explain the cause of ‘aging’; however, the factors that affect this complex process are still poorly understood. Of these theories, the accumulation of oxidative damage over time is among the most accepted. Particularly, the heart is one of the most affected organs by oxidative stress. The current study, therefore, aimed to investigate oxidative stress markers in myocardial tissue of rats at different ages.Methods: Seventy-two rats were distributed into 6 groups of 12 animals each and maintained for 3, 6, 9, 12, 18 and 24 months. After euthanasia, the heart was removed and the levels of non-protein thiols, lipid peroxidation, and protein carbonylation, as well as superoxide dismutase and catalase activities were determined.Results:Superoxide dismutase, catalase activity and lipid peroxidation were reduced in the older groups of animals, when compared with the younger group. However, protein carbonylation showed an increase in the 12-month group followed by a decrease in the older groups. In addition, the levels of non-protein thiols were increased in the 12-month group and not detected in the older groups.Conclusion:Our data showed that oxidative stress is not associated with aging in the heart. However, an increase in non-protein thiols may be an important factor that compensates for the decrease of superoxide dismutase and catalase activity in the oldest rats, to maintain appropriate antioxidant defenses against oxidative insults.

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Impact of Plasma Oxidative Stress Markers on Post-race Recovery in Ultramarathon Runners: A Sex and Age Perspective Overview

Antioxidants ◽

10.3390/antiox10030355 ◽

2021 ◽

Vol 10 (3) ◽

pp. 355

Author(s):

Carlos Guerrero ◽

Eladio Collado-Boira ◽

Ignacio Martinez-Navarro ◽

Barbara Hernando ◽

Carlos Hernando ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Enzymes ◽

Oxidative Damage ◽

Antioxidant Defenses ◽

Lipid Peroxidation Product ◽

Finish Line ◽

Stress Markers ◽

Markers Of Oxidative Stress ◽

The Impact ◽

Plasma Markers

Oxidative stress has been widely studied in association to ultra-endurance sports. Although it is clearly demonstrated the increase in reactive oxygen species and free radicals after these extreme endurance exercises, the effects on the antioxidant defenses and the oxidative damage to macromolecules, remain to be fully clarified. Therefore, the aim of this study was to elucidate the impact of an ultramarathon race on the plasma markers of oxidative stress of 32 runners and their post-race recovery, with especial focused on sex and age effect. For this purpose, the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR) activity, as well as the lipid peroxidation product malondialdehyde (MDA) and the carbonyl groups (CG) content were measured before the race, in the finish line and 24 and 48 h after the race. We have reported an increase of the oxidative damage to lipids and proteins (MDA and CG) after the race and 48 h later. Moreover, there was an increase of the GR activity after the race. No changes were observed in runners’ plasma GPx activity throughout the study. Finally, we have observed sex and age differences regarding damage to macromolecules, but no differences were found regarding the antioxidant enzymes measured. Our results suggest that several basal plasma markers of oxidative stress might be related to the extent of muscle damage after an ultraendurance race and also might affect the muscle strength evolution.

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Effect of Tai Chi on Markers of Oxidative Stress: Systematic Review and Meta-Analysis

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph18073458 ◽

2021 ◽

Vol 18 (7) ◽

pp. 3458

Author(s):

Juana Rosado-Pérez ◽

Osvaldo D. Castelán-Martínez ◽

Abril J. Mújica-Calderón ◽

Martha A. Sánchez-Rodríguez ◽

Víctor Manuel Mendoza-Núñez

Keyword(s):

Oxidative Stress ◽

Systematic Review ◽

Superoxide Dismutase ◽

Sedentary Behavior ◽

Tai Chi ◽

Meta Analysis ◽

Cochrane Library ◽

Stress Markers ◽

Markers Of Oxidative Stress ◽

Mean Differences

Background: This study aimed to synthesize the evidence of the effect of practicing Tai Chi on oxidative stress markers (OxSM). Methods: This systematic review and meta-analysis was conducting using the MEDLINE, Cochrane Library, ScienceDirect, Scopus, Epistemonikos, Lilacs, and Ovid databases to identify randomized (RCT) and non-randomized (NRCT) clinical trials that evaluated the Tai Chi effect on OxSM compared to sedentary behavior, walking or yoga. Pooled mean differences (MDs) with 95% confidence intervals (95%CI) were estimated using the inverse variance method to determine the effect of Tai Chi on OxSM. PROSPERO register: CRD42019138362. Results: Five RCT and five NRCT were included. Compared to sedentary behavior, regular Tai Chi practice increases the levels of the enzymes superoxide dismutase (MD = 34.97 U/mL, (95%CI, 9.45 to 60.48), 344 participants) and catalase (MD = 15.63 U/mL, (95%CI, 4.05 to 27.22), 110 participants), as well as reducing the levels of lipoperoxides (MD = −0.02 µmol/L, (95%CI, −0.04 to −0.00), 234 participants). For comparisons with walking or yoga, only one study per activity was identified comparing the effect on OxSM. Conclusions: Regular Tai Chi practice increases the levels of superoxide dismutase and catalase, as well as reducing the levels of lipoperoxides. More studies are necessary to determine the effect of Tai Chi on OxSM when compared to other physical activities.

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Differential susceptibility of a few members of the nasuta–albomicans complex of Drosophila to paraquat-induced lethality and oxidative stress

Genome ◽

10.1139/g11-049 ◽

2011 ◽

Vol 54 (10) ◽

pp. 829-835 ◽

Cited By ~ 2

Author(s):

Mysore S. Ranjini ◽

Ravikumar Hosamani ◽

Muralidhara ◽

Nallur B. Ramachandra

Keyword(s):

Oxidative Stress ◽

Biochemical Analysis ◽

Reduced Glutathione ◽

Differential Susceptibility ◽

Activity Levels ◽

Oxygen Species ◽

Stress Markers ◽

The Status ◽

Markers Of Oxidative Stress ◽

And Oxidative Stress

The evolution of karyotypically stabilized short-lived (SL) and long-lived (LL) cytoraces in the laboratory have been established and validated through our previous lifespan studies. In the present investigation, we examined the possible reason(s) for the differential longevity among selected members of SL and LL cytoraces, employing the well known paraquat (PQ) resistance bioassay. Exposure of these races to varying concentrations of PQ revealed relatively higher resistance among LL cytoraces than SL cytoraces, as evident by the lower incidence of mortality. Biochemical analysis for endogenous markers of oxidative stress revealed that LL-2 cytorace exhibited lower reactive oxygen species (ROS) and lipid peroxidation (LPO) levels, higher activity levels of superoxide dismutase (SOD), and coupled with higher levels of reduced glutathione (GSH) compared with the levels found in SL-2 cytorace. These findings suggest that the higher susceptibility of SL cytoraces to PQ challenge may be, at least in part, related to the higher endogenous levels of oxidative stress markers. Although the precise mechanisms responsible for the longer longevity among LL cytoraces of the nasuta–albomicans complex of Drosophila merits further investigation, our data suggest that the relatively longer lifespan may be related to the status of endogenous markers that renders them more resistant towards oxidative-stress-mediated lethality, as evident in the PQ assay.

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Photobiomodulation Leads to Reduced Oxidative Stress in Rats Submitted to High-Intensity Resistive Exercise

Oxidative Medicine and Cellular Longevity ◽

10.1155/2018/5763256 ◽

2018 ◽

Vol 2018 ◽

pp. 1-9 ◽

Cited By ~ 4

Author(s):

Helenita Antonia de Oliveira ◽

Ednei Luiz Antonio ◽

Gisela Arsa ◽

Eduardo Tadeu Santana ◽

Flávio André Silva ◽

...

Keyword(s):

Oxidative Stress ◽

Antioxidant Capacity ◽

High Intensity ◽

Maximum Load ◽

Exercise Session ◽

Time Interval ◽

Oxidative Stress Markers ◽

Resistive Exercise ◽

Stress Markers ◽

Low Intensity Laser Therapy

The aim of this study was to determine whether oxidative stress markers are influenced by low-intensity laser therapy (LLLT) in rats subjected to a high-intensity resistive exercise session (RE). Female Wistar rats divided into three experimental groups (Ctr: control, 4J: LLLT, and RE) and subdivided based on the sampling times (instantly or 24 h postexercise) underwent irradiation with LLLT using three-point transcutaneous method on the hind legs, which was applied to the gastrocnemius muscle at the distal, medial, and proximal points. Laser (4J) or placebo (device off) were carried out 60 sec prior to RE that consisted of four climbs bearing the maximum load with a 2 min time interval between each climb. Lipoperoxidation levels and antioxidant capacity were obtained in muscle. Lipoperoxidation levels were increased (4-HNE and CL markers) instantly post-RE. LLLT prior to RE avoided the increase of the lipid peroxidation levels. Similar results were also notified for oxidation protein assays. The GPx and FRAP activities did not reduce instantly or 24 h after RE. SOD increased 24 h after RE, while CAT activity did not change with RE or LLLT. In conclusion, LLLT prior to RE reduced the oxidative stress markers, as well as, avoided reduction, and still increased the antioxidant capacity.

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Carotid intima-media thickness and oxidative stress markers for assessment of atherosclerosis in children with β thalassemia major

Thalassemia Reports ◽

10.4081/thal.2016.4939 ◽

2016 ◽

Vol 6 (1) ◽

Author(s):

Geetanjali Jindal ◽

Prashant Chavan ◽

Ravinder Kaur ◽

Shivani Jaswal ◽

Kamal Kumar Singhal ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Oxidized Ldl ◽

Thalassemia Major ◽

Lipid Peroxidation Product ◽

Oxidative Stress Markers ◽

Lipoprotein A ◽

Stress Markers ◽

Total Antioxidant ◽

Peroxidation Product

The present study evaluates carotid intimamedia thickness (CIMT) in children with β thalassemia major to assess atherosclerosis and its relation to the underlying proposed causative mechanisms via lipid peroxidation product malondialdehyde (MDA), oxidized lowdensity lipoproteins (LDL), total antioxidant level, and lipid profile. A cross sectional study was conducted on 62 children (31 cases and 31 controls). CIMT by high resolution ultrasound and biochemical parameters i.e., total cholesterol, triglycerides, high-density lipoproteins, LDL, Oxidized LDL, lipoprotein (a), lipid peroxidation product MDA and total antioxidant were measured in enrolled subjects and compared. In our study, CIMT was significantly increased in β thalassemia major patients’ as compared to healthy controls. Mean CIMT in cases was 0.69±0.11 mm and in controls 0.51±0.07 mm. Mean oxidized LDL (EU/mL) in cases 39.3±34.4 (range 14.4 to 160) was significantly raised (P=0.02, t test) as compared to controls 23.9±13.4 (range 12 to 70). In our study we found MDA levels (nmol/mL) to be increased in β thalassemia patients as compared to controls. Mean MDA was 10.0±3.27 (4.41 to 17.48) in cases while in controls was 6.87±4.55 (1.5 to 17.9). Our study results show CIMT as an early marker of atherogenesis in β thalassemia major. Oxidative stress markers are also increased in β thalassemia major patients and lipoprotein (a) shows a positive correlation with CIMT. The present study points towards various atherogenetic mechanisms in β thalassemia major. 本研究评价β重型地中海贫血患儿颈动脉内膜中层厚度（CIMT），以评估动脉粥样硬化，以及与潜在通过血脂过氧化反应产物丙二醛（MDA）、氧化低密度脂蛋白（LDL）、总抗氧化水平和血脂谱所提出致病机制之间的关系。在62名儿童（31例病例和31例对照）中进行了一项横断面研究。在入组受试者中通过高分辨率超声和生化指标（即总胆固醇、甘油三酯、高密度脂蛋白、LDL、氧化LDL，脂蛋白（a）、血脂过氧化产物MDA和总抗氧化剂）测量CIMT并进行比较。在我们的研究中，CIMT在β重型地中海贫血患者中比健康对照组显著增加。病例组中的平均CIMT为0.69±0.11 mm，对照组0.51±0.07 mm。病例组中平均氧化LDL（EU/mL）为39.3±34.4（从14.4到160的范围）与对照组的23.9±13.4（12至70的范围）相比显著升高（P = 0.02，t检验）。在我们的研究中，我们发现β地中海贫血患者中的MDA水平（nmol/mL）比对照组更高。病例组中的平均MDA为10.0±3.27（4.41至17.48），而对照组为6.87±4.55（1.5到17.9）。我们的研究结果表明，CIMT是β重型地中海贫血动脉粥样硬化的早期标记物。氧化应激标记物在β重型地中海贫血患者中也有增加，脂蛋白（a）显示出与CIMT呈正相关。本研究针对β重型地中海贫血中的各种动脉粥样硬化机制。

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Roles of Superoxide dismutase(SOD) , Malondialdehyde (MDA), 8-iso-prostaglandinF2α ( 8-iso-PGF2α)as oxidative stress in development and progression of Brest cancer in Iraqi females patients

Al-Qadisiyah Journal Of Pure Science ◽

10.29350/jops.2020.25.1.1053 ◽

2020 ◽

Vol 25 (1) ◽

pp. 1-4

Author(s):

Ferdous Abbas Jabir ◽

Ahmed Sabah Shaker

Keyword(s):

Breast Cancer ◽

Oxidative Stress ◽

Cancer Patients ◽

Antioxidant Defenses ◽

Blood Levels ◽

P Value ◽

Oxygen Species ◽

Breast Cancer Patients ◽

Markers Of Oxidative Stress ◽

The Mean

Oxidative stress occurs as a result of disturbance in the balance between the production of reactive oxygen species (free radicals) and antioxidant defenses. Markers of oxidative stress were measured the markers of oxidative stress in breast cancer patients after diagnosis of breast cancer and compared these plasma blood levels controls This study was conducted to three markers of oxidative stress ;these are (SOD) enzyme ,malondialdehyde (MDA)and8-iso-prostaglandinF2α plasma of patients with breast cancer and compare with controls .In this study ; the mean MDA (ng/ml) levels for the breast cancer patients and the controls were55.91±3.31 and40.61±3.76 respectively, while the SOD (pg/ml) levels were1530.37±80.4 and1851.4 9±93.65 respectively and the 8-iso-PGF2α (ng/ml ) levels were 40.16±3.31 and 30.16±2.34 difference of the mean were statistically significant (p value <0.05).

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Salivary Oxidative Stress Markers’ Relation to Oral Diseases in Children and Adolescents

Antioxidants ◽

10.3390/antiox10101540 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1540

Author(s):

Bahareh Nazemi Salman ◽

Shayan Darvish ◽

Ancuta Goriuc ◽

Saeideh Mazloomzadeh ◽

Maryam Hossein Poor Tehrani ◽

...

Keyword(s):

Oxidative Stress ◽

Dental Caries ◽

Pediatric Population ◽

Current Evidence ◽

Oxidative Stress Markers ◽

Oral Diseases ◽

Stress Markers ◽

Gingival Bleeding ◽

Whole Saliva ◽

Markers Of Oxidative Stress

(1) Background: Current evidence suggests that salivary markers of oxidative stress are indicative of clinical disease indices such as the papillary bleeding index (PBI) and the caries index (CI). The aim of this study was to assess the relation of oxidative stress markers with oral dental caries and periodontal problems in a pediatric population. (2) Methods: In this case-control study, unstimulated whole saliva was collected from individuals aged 3–18 years (n = 177); 14 individuals were excluded. Study subjects were divided into those with caries (CI = 2, n = 78) and those who were caries-free (n = 85). These groups were then divided into another subset consisting of children (mean age 7.3 years, n = 121) and adolescents (mean age 16.1 years, n = 42). The PBI was determined in all groups. We then assessed salivary levels of oxidative stress markers. (3) Results: Total antioxidant capacity (TAC) level increased in patients with more gingival bleeding (p < 0.05) in the study group aged 3–18 years. In addition, TAC showed a significant decrease in samples with caries when compared to the caries-free group in adolescents (p = 0.008). (4) Conclusions: TAC levels may be a marker of both gingival bleeding and dental caries in young adult populations. We hope that in the near future, prophylaxis, control, follow up and even possible therapeutic use of oxidative stress markers in a chairside way will become possible as antioxidants have been shown to be effective against oral diseases.

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The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

Archives of Biological Sciences ◽

10.2298/abs1403075s ◽

2014 ◽

Vol 66 (3) ◽

pp. 1075-1081

Author(s):

Ivan Simic ◽

Violeta Iric-Cupic ◽

Rada Vucic ◽

Marina Petrovic ◽

Violeta Mladenovic ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Rat Brain ◽

Wistar Rats ◽

Superoxide Radical ◽

Reduced Glutathione ◽

Oxidative Stress Markers ◽

Sod Activity ◽

Stress Markers ◽

Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

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