Feed Fermentation with Reuteran- and Levan-Producing Lactobacillus reuteri Reduces Colonization of Weanling Pigs by Enterotoxigenic Escherichia coli
ABSTRACTThis study determined the effect of feed fermentation withLactobacillus reuterion growth performance and the abundance of enterotoxigenicEscherichia coli(ETEC) in weanling piglets.L. reuteristrains produce reuteran or levan, exopolysaccharides that inhibit ETEC adhesion to the mucosa, and feed fermentation was conducted under conditions supporting exopolysaccharide formation and under conditions not supporting exopolysaccharide formation. Diets were chosen to assess the impact of organic acids and the impact of viableL. reuteribacteria. Fecal samples were taken throughout 3 weeks of feeding; at the end of the 21-day feeding period, animals were euthanized to sample the gut digesta. The feed intake was reduced in pigs fed diets containing exopolysaccharides; however, feed efficiencies did not differ among the diets. Quantification ofL. reuteriby quantitative PCR (qPCR) detected the two strains used for feed fermentation throughout the intestinal tract. Quantification ofE. coliand ETEC virulence factors by qPCR demonstrated that fermented diets containing reuteran significantly (P< 0.05) reduced the copy numbers of genes forE. coliand the heat-stable enterotoxin in feces compared to those achieved with the control diet. Any fermented feed significantly (P< 0.05) reduced the abundance ofE. coliand the heat-stable enterotoxin in colonic digesta at 21 days; reuteran-containing diets reduced the copy numbers of the genes forE. coliand the heat-stable enterotoxin below the detection limit in samples from the ileum, the cecum, and the colon. In conclusion, feed fermentation withL. reuterireduced the level of colonization of weaning piglets with ETEC, and feed fermentation supplied concentrations of reuteran that may specifically contribute to the effect on ETEC.