The volatile exudates from germinating pea seeds of different viability and vigor

1985 ◽  
Vol 63 (6) ◽  
pp. 1035-1039 ◽  
Author(s):  
R. J. Gorecki ◽  
G. E. Harman ◽  
L. R. Mattick
Keyword(s):  
Gas Chromatography ◽  
Relative Humidity ◽  
Trichoderma Harzianum ◽  
Storage Time ◽  
Enterobacter Cloacae ◽  
Storage Condition ◽  
Seed Vigor ◽  
Embryonic Axes ◽  
Pea Seeds ◽  
Germinating Seeds

Pea seeds var. Kriter were stored aseptically at 92% relative humidity and 30 °C. After 0, 4, 6, 8, or 10 weeks of storage, viability, vigor, and volatile exudates were determined on sublots of seeds. As storage time increased, vigor, as measured by dehydrogenase activity, growth of embryonic axes, and conductivity decreased. Later, viability also decreased. Imbibing and germinating pea seeds produced ethanol, acetaldehyde, and lesser amounts of methanol. No qualitative differences in volatile exudates were observed from germinating seeds regardless of age or storage condition. Nonaged seeds with highest vigor produced the smallest amounts of volatiles, but with increased aging the quantities of ethanol and acetaldehyde gradually increased. Dry seed produced small quantities of both volatiles. The amount of these compounds produced reached a maximum between 12 and 48 h of germination. Infestation of seed samples with Enterobacter cloacae or Trichoderma harzianum reduced the quantities of these compounds measured. These results indicate that determinations of acetaldehyde and ethanol in the space over germinating seeds by means of gas chromatography may be a useful seed vigor test.

scholarly journals Responses of quinoa (Chenopodium quinoa Willd.) seeds stored under different germination temperatures

2017 ◽  
Vol 39 (1) ◽  
pp. 83 ◽  
Author(s):  
Andressa Strenske ◽  
Edmar Soares de Vasconcelos ◽  
Vanessa Aline Egewarth ◽  
Neusa Francisca Michelon Herzog ◽  
Marlene De Matos Malavasi
Keyword(s):  
Room Temperature ◽  
Storage Time ◽  
Chenopodium Quinoa ◽  
Seed Vigor ◽  
Continuous Darkness ◽  
Experimental Conditions ◽  
Growing Seasons ◽  
Germinating Seeds ◽  
Germinated Seeds ◽  
Plot Design

In this experiment, we assessed the germination and vigor of quinoa seeds packed in paper bags and stored at room temperature for 36, 85, 119, 146, 177 and 270 days. The seeds were harvested under experimental conditions in Marechal Candido Rondon, Paraná, during the 2012/13 growing seasons. Four replicates of 100 seeds each were established for each storage time, and the seeds were evaluated, on paper, based on the BOD under the following experimental temperature conditions: alternating temperatures of 20 and 30°C and a constant temperature of 25°C. The seeds from both treatments were subject to seven-hour photoperiods and 25°C under continuous darkness. The germinated seeds were counted daily for eight days after sowing, and we evaluated the percentages of normal and abnormal seedlings and the germination index. The experimental design was completely randomized using a split-plot design. Increasing the storage time decreased the percentage of germinated seeds and seed vigor due to the increased number of abnormal seedlings. Over the 430-day study period, quinoa seed germination completely declined under the experimental conditions. The final number of germinating seeds should be evaluated 7 days after the beginning of the germination test. 

scholarly journals Proteins in pea seeds after 7 months of storage at 50 and 90% relative humidity

2014 ◽  
Vol 54 (4) ◽  
pp. 367-378
Author(s):  
Ryszard J. Górecki ◽  
Andrzej Klasa ◽  
Artur Bałajewicz
Keyword(s):  
Relative Humidity ◽  
Gel Electrophoresis ◽  
Room Temperature ◽  
Polyacrylamide Gel Electrophoresis ◽  
Electrophoretic Pattern ◽  
Embryonic Axes ◽  
Albumin Content ◽  
Pea Seeds ◽  
Viable Seeds ◽  
Pea Seed

Two pea seed lots var. Flavanda were stored at 50 and 90% relative humidity (r.h.) at room temperature. After 7 months the germinability of seeds stored at 90% r.h. was reduced to 19%;. Similarly, the vigor of these seeds was lower as compared with seeds stored at 50% r.h. Deterioration of seeds resulted in the reduction of the albumin content from about 14 to 8 mg per seed. The content of vicillin in non-viable seeds was twice as high than in fully vigorous ones. No changes in the level of legumin appeared. During germination of seeds with lowered viability and vigor, the protein content of their embryonic axes did not increase. Polyacrylamide gel electrophoresis of protein extracted from the cotyledons of aged seeds showed visible changes only in the electrophoretic pattern of the albumin fraction.

Physiological changes in the early stages of germination of pea seeds induced by aging and by infection by a storage fungus, Aspergillus ruber

1976 ◽  
Vol 54 (1-2) ◽  
pp. 39-44 ◽  
Author(s):  
G. E. Harman ◽  
A. A. Khan ◽  
K. L. Tao
Keyword(s):  
Alkaline Phosphatase ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Relative Humidity ◽  
Physiological Changes ◽  
Embryonic Axes ◽  
Storage Fungi ◽  
Storage Fungus ◽  
Pea Seeds ◽  
Reduced Activity

Pea seeds age rapidly and may be infected by storage fungi, e.g. Aspergillus ruber, when stored under conditions of high relative humidity and temperature (92% relative humidity and 30 °C in these experiments). In the absence of microorganisms, pea seeds retain their viability for 6 to 8 weeks, although the speed of germination is reduced. In embryonic axes, aging is associated with a slower increase in O2 uptake, a delay in protein synthesis, smaller ATP and amino acid pools, and reduced activity of alkaline phosphatase (EC 3.1.3.1) and 3-phosphoglyceraldelyde dehydrogenase (EC 1.2.1.12). Axes recover from these changes during germination. Infection by A. ruber reduces germination. In axes from infected peas the onset of protein synthesis is delayed even more and the ATP and amino acid pools are smaller than in aged axes. Axes from infected seeds recover slowly, if at all.

scholarly journals PHYSIOLOGICAL AND BIOCHEMICAL ALTERATIONS ON THE STORAGE OF Cedrela fissilis VELLOZO SEEDS

FLORESTA ◽  
2018 ◽  
Vol 48 (1) ◽  
pp. 01 ◽  
Author(s):  
Andressa Vasconcelos Flores ◽  
Glauciana Da Mata Ataíde ◽  
Eduardo Euclydes de Lima Borges ◽  
Renato Vinícius Oliveira Castro ◽  
Márcio Dias Pereira
Keyword(s):  
Electrical Conductivity ◽  
Relative Humidity ◽  
Storage Time ◽  
Ex Situ Conservation ◽  
Seed Vigor ◽  
Ex Situ ◽  
Biochemical Alterations ◽  
Germination Vigor ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Physiological And Biochemical

 In order to ensure the ex-situ conservation of a species, knowing the behavior of the seed during storage is necessary, as it is influenced by several factors inherent to the seed and by characteristics of the environment, such as temperature and relative humidity. This study aimed to evaluate the physiological and biochemical alterations on Cedrela fissilis (pink cedar) seeds during storage under different environmental conditions. Seeds were stored at 20ºC in desiccators containing salts that established the relative humidity of 34%, 55%, 75%, and 93%. Analysis were performed on fresh seeds (without storage - time zero), which were used as control, and every two months through a period of eight months of storage. At each assessment, the following aspects were quantified: water content; lipid peroxidation; and the activities of catalase and glucose-6-phosphate dehydrogenase. Germination and electrical conductivity tests were analysed. Seed vigor decreased on humidity at 20 °C. It correlated mainly with decrease of glucose-6-phosphate dehydrogenase activity and increase of lipid peroxidation.Keywords: Pink cedar; germination; vigor; enzymes, lipids. ResumoAlterações fisiológicas e bioquímicas durante o armazenamento de sementes de Cedrela fissilis Vellozo. Para garantir a conservação ex-situ de uma espécie, é necessário conhecer o comportamento das sementes durante o armazenamento, visto que este é influenciado por vários fatores inerentes à semente e às características do ambiente, tais como temperatura e umidade relativa. Este estudo teve como objetivo avaliar as alterações fisiológicas e bioquímicas em sementes de Cedrela fissilis (cedro rosa) durante o armazenamento sob diferentes condições ambientais. As sementes foram armazenadas a 20ºC em dessecadores contendo sais que estabeleceram as umidades relativas do ar de 34%, 55%, 75% e 93%. As análises foram realizadas em sementes frescas (sem armazenamento - tempo zero), usadas como controle, e a cada dois meses durante um período de oito meses de armazenamento. Em cada avaliação, foram quantificados: teor de água; peroxidação de lípidos; e as atividades das enzimas catalase e glicose-6-fosfato desidrogenase. Também, foi analisada a germinação e condutividade elétrica das sementes. O vigor das sementes diminuiu em todas as umidades a 20 °C, correlacionando-se, principalmente, com a diminuição na atividade de glicose-6-fosfato desidrogenase e com o aumento na peroxidação lipídica.Palavras-chave: Cedro rosa; germinação; vigor; enzimas, lipídios.

scholarly journals Effects of Sources and Forms of Vitamin K3 on Its Storage Stability in Vitamin Premixes or Vitamin Trace Mineral Premixes

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1140
Author(s):  
Huakai Wang ◽  
Pan Yang ◽  
Longxian Li ◽  
Nan Zhang ◽  
Yongxi Ma ◽  
...  
Keyword(s):  
Relative Humidity ◽  
Storage Time ◽  
Storage Stability ◽  
Choline Chloride ◽  
Crystal Form ◽  
Negative Effects ◽  
Trace Mineral ◽  
Vitamin K3 ◽  
Choline Group ◽  
Micro Sphere

Six types of vitamin K3 (VK3); two sources (menadione sodium bisulfite, MSB; menadione nicotinamide bisulfite, MNB), and three different forms (crystal, micro-capsule, and micro-sphere) were used to determine the retention of VK3 in vitamin premixes (Experiment 1) or vitamin trace mineral (VTM) premixes (Experiment 2) after 1, 2, 3, and 6 months of storage. The retention of VK3 in vitamin premixes was evaluated at 25 °C/60% relative humidity or 40 °C/75% relative humidity in an incubator in Experiment 1 and in VTM premixes (choline chloride: 0 vs. 16,000 mg/kg) stored at room temperature in Experiment 2. The VK3 retention in vitamin premix or VTM premix decreased significantly with the extension of storage time (p < 0.05). In Experiment 1, the VK3 retention was higher in the 25 °C/60% incubator (56%) than in the 40 °C/75% incubator (28%). The MNB retention (52%) was higher than MSB retention (32%). The retention of VK3 in micro-capsules (43%) or micro-spheres (48%) was higher than the crystal form (35%) after six months of storage. In Experiment 2, there was no difference between the retention of MSB (49%) or MNB (47%). The retention of VK3 of micro-capsule (51%) or micro-sphere (54%) was higher than that of crystal form (40%). The VK3 retention was higher in the choline-free group (51%) than in the choline group (47%) after six months of storage. Finally, the predicted equations of VK3 retention with storage time in vitamin premixes or VTM premixes were established. The R2 of the prediction equations was ≥0.9005, indicating that time is an important factor in predicting VK3 retention. In conclusion, the higher temperature-relative humidity, choline had negative effects on VK3 retention during premix storage. MNB retention was higher than MSB during storage of vitamin premix. The encapsulated forms of VK3, micro-capsules and micro-spheres, could improve VK3 storage stability in vitamin premix and VTM premix.

scholarly journals Assessment of lettuce quality during storage at low relative humidity using Global Stability Index methodology

2012 ◽  
Vol 32 (2) ◽  
pp. 366-373 ◽  
Author(s):  
María Roberta Ansorena ◽  
María Victoria Agüero ◽  
María Grabriela Goñi ◽  
Sara Roura ◽  
Alejandra Ponce ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Relative Humidity ◽  
Global Stability ◽  
Water Content ◽  
Relative Water Content ◽  
Storage Time ◽  
Stability Index ◽  
Total Chlorophyll ◽  
External Zone ◽  
Relative Water

During postharvest, lettuce is usually exposed to adverse conditions (e.g. low relative humidity) that reduce the vegetable quality. In order to evaluate its shelf life, a great number of quality attributes must be analyzed, which requires careful experimental design, and it is time consuming. In this study, the modified Global Stability Index method was applied to estimate the quality of butter lettuce at low relative humidity during storage discriminating three lettuce zones (internal, middle, and external). The results indicated that the most relevant attributes were: the external zone - relative water content, water content , ascorbic acid, and total mesophilic counts; middle zone - relative water content, water content, total chlorophyll, and ascorbic acid; internal zone - relative water content, bound water, water content, and total mesophilic counts. A mathematical model that takes into account the Global Stability Index and overall visual quality for each lettuce zone was proposed. Moreover, the Weibull distribution was applied to estimate the maximum vegetable storage time which was 5, 4, and 3 days for the internal, middle, and external zone, respectively. When analyzing the effect of storage time for each lettuce zone, all the indices evaluated in the external zone of lettuce presented significant differences (p < 0.05). For both, internal and middle zones, the attributes presented significant differences (p < 0.05), except for water content and total chlorophyll.

measurements. This paper is confined to the different forms of sampling odourous gases for olfactometric measurements and the problems involved. It refers to existing guidelines for olfactometric measurements in the countries of the EEC, as well. 2. TYPES OF SAMPLING Samples of odourous gas may be collected in unconcentrated or concentrated form. Concentrated sampling is usually neces­ sary when gas chromatography or other chemical analytical meth­ ods are to be used. Unconcentrated sampling is provided if o-dour threshold concentrations are required (2). Depending on the type of olfactometer used dynamic sam­ pling or static sampling are provided. The principle of dynam­ ic sampling is shown in Figure 1. It requires a part-flow of the odourous gas to be continoulsy extracted from the source and subsequently directed to the olfactometer. This sampling method implies that the measurements are carried out close to the source. An advantage of the method is that there is the possibility of controlling a process, directly, and in case of the break-down of the process this can be noticed right away. A disadvantage of the dynamic method is that odour sources that are not readily accessible require a relatively great ef­ fort in order to install the olfactometer and suitable sam­ pling pipes which often should be insulated or heated to avoid adsorption or condensation (3). When static sampling is used a partial stream of the o-dourous air is collected in a sampling vessel. Samples are taken from this vessel or bag to dilute the odourous air for the olfactometer using syringes or on-line tubings. When using this method odour measurement with the panel can be carried out at any arbitrary location, if the vessel is a transport­ able one. An example for static sampling is given in Figure 2. 3. PROBLEMS OF SAMPLING the main problems encountered when sampling odourous air derive from surface effects of the sampling tubes and vessels, namely by - adsorption, - desorption, and - condensation. This depends mainly on the material of the tube, the vessel or the bag (adsorption) or on the nature of the gas, whether it is hot and/or containes a high amount of humidity (condensa­ tion). On the other hand the sample can be altered by trace components bleeding from the material of the walls of the ves­ sel or the tube (desorption). The following factors are to be observed for valid static sampli ng. aTTTToTce of_m£teri aj_ For tWe sampling of odourous gases glas vessels, stain­ less steel tanks (4) and flexible plastic bags (5) were tested. The initial concentrations of the test gases decrease consider­ ably with storage time in glass and steel vessels. In recent years bags made of Polyethylene(6), Teflon (3) and Tedlar (7), (8) were usually used. Figure 3 shows a graph from SCHUETZLE

1986 ◽  
pp. 59-59
Keyword(s):  
Gas Chromatography ◽  
Storage Time ◽  
Dynamic Method ◽  
The Other ◽  
Plastic Bags ◽  
Steel Tanks ◽  
On Line ◽  
Arbitrary Location ◽  
Adsorption Desorption ◽  
Trace Components

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

Thermoinhibition alters the polyamine levels in cotyledons and embryonic axes during germination of stratified chick-pea seeds

Plant Science ◽  
1994 ◽  
Vol 101 (2) ◽  
pp. 143-150 ◽  
Author(s):  
Paloma Muñoz de Rueda ◽  
Angel Jesús Matilla ◽  
Isabel María Sanchez-Calle
Keyword(s):  
Embryonic Axes ◽  
Chick Pea ◽  
Pea Seeds

Action of nitric oxide on the physiological potential and biochemical mechanisms of pea seeds

2021 ◽  
Vol 43 ◽  
Author(s):  
Marcelo Coelho Sekita ◽  
Denise Cunha Fernandes dos Santos Dias ◽  
Daniel Teixeira Pinheiro ◽  
Aparecida Leonir da Silva ◽  
Antônio César Batista Matos ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Seed Germination ◽  
Seed Vigor ◽  
Water Control ◽  
No Donor ◽  
Germination Process ◽  
Physiological Quality ◽  
Pea Seeds ◽  
Germination Vigor ◽  
Pea Seed

Abstract: Nitric oxide (NO) can act in biochemical pathways of the germination process; however, there is little information about how it acts on the performance of pea seeds. The aim of this study was to evaluate the physiological and biochemical effects of NO on pea seed germination and vigor. Pea seeds cv. Itapuã 600 obtained from three seed lots with different levels of physiological quality were sown in a substrate moistened with water (control) or sodium nitroprusside (SNP) solution, a NO donor (50 μM), to assess germination, vigor, activity of antioxidant enzymes, reactive oxygen species, lipid peroxidation, and amylase activity. NO application does not alter pea seed germination, but it increases vigor. It is more effective in seeds with lower physiological potential. In addition, NO leads to reduction in oxidative stress, favors the translocation of reserves to the embryo, and has potential for use in the treatment of pea seeds to increase seed vigor.

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