Cryopreservation of Prunus padus seeds: emphasising the significance of Bayesian methods for data analysis

2016 ◽  
Vol 46 (6) ◽  
pp. 766-774 ◽  
Author(s):  
Elena Popova ◽  
Elena Moltchanova ◽  
Sim Hee Han ◽  
Praveen Saxena ◽  
Du Hyun Kim
Keyword(s):  
No Reduction ◽  
Seedling Emergence ◽  
Seed Viability ◽  
Seed Storage ◽  
Prunus Species ◽  
Fresh Weight Basis ◽  
Prunus Padus ◽  
Seed Conservation ◽  
Effective Conservation ◽  
Direct Immersion

Conservation of Prunus padus L., a tree of high ecological and pharmacological importance, has been evaluated by storing seeds at subzero (−20 °C and −80 °C) and cryogenic (−196 °C) temperatures for various durations. The effect of the seed’s water content (WC) ranging from 3.5% to 21.1%, fresh weight basis, and the effect of cooling and rewarming procedures on seed viability was investigated. Emergence of seedlings was observed for 40%–55% of the noncryopreserved seeds, with no significant effect of WC. The same seedling emergence was recorded for seeds cryopreserved by direct immersion in liquid nitrogen within the WC range of 3.5%–15.0%. Seeds rehydrated above 17% WC were unable to tolerate cryopreservation. Seedling emergence was not affected by cooling regime but decreased by 10% after stepwise rewarming compared with rapid rewarming in a water bath or on air. No reduction in seedling emergence was recorded after storage at −20 °C, −80 °C, and −196 °C for 1 h, 1 week, and 1 month. We recommend seed storage at subzero or cryogenic temperatures as an effective conservation option for P. padus and possibly other Prunus species. We also demonstrated high effectiveness and reliability of Bayesian statistical methods for analyzing binomial data such as the data obtained in seed conservation experiments.

scholarly journals Temperature and Relative Humidity Govern Germination and Storage of Gladiolus Seed

HortScience ◽  
1991 ◽  
Vol 26 (8) ◽  
pp. 1054-1057 ◽  
Author(s):  
W.J. Carpenter ◽  
G.J. Wilfret ◽  
J.A. Cornell
Keyword(s):  
Relative Humidity ◽  
Seed Treatment ◽  
No Reduction ◽  
Germination Rate ◽  
Seed Viability ◽  
Seed Storage ◽  
Moisture Contents ◽  
Seed Moisture ◽  
Germination Temperature ◽  
And Storage

Gladiolus (G. grandiflorus) seed germination was light-independent, but temperature influenced the germination rate. Constant 20C promoted higher total germination (97%), fewer days (4.3) to 50% of final germination, and shorter span of days (4.8) between 10% and 90% germination than other constant temperatures, although similar results were achieved by alternating 12-h cycles of 20 to 25C. Total germination was unchanged after seed treatment for 7 days at 10 to -20C, but longer germination periods were required after treatments below -10C. Reducing seed moisture contents from 11.8% to 4.2% caused no reduction in total germination, but moisture contents below 6.6% delayed achieving 50% of final germination and extended the periods from 10% to 90% of germination. Temperature and relative humidity (RH) during storage were important in retaining seed viability, with RH having a larger effect. Smallest declines in total germination during 12 months of storage occurred at 11% and 33% RH at 15C. The statistical analysis estimated the optimum seed storage at 14C and 26% RH.

scholarly journals Effect of different conditions of storage on seed viability and seedling growth of six European wild fruit woody plants

2020 ◽  
Vol 77 (2) ◽  
Author(s):  
Mikołaj Krzysztof Wawrzyniak ◽  
Marcin Michalak ◽  
Paweł Chmielarz
Keyword(s):  
Seedling Growth ◽  
Prunus Avium ◽  
Seedling Emergence ◽  
Seed Viability ◽  
Storage Conditions ◽  
Sorbus Aucuparia ◽  
Prunus Padus ◽  
Wild Fruit ◽  
Malus Sylvestris ◽  
Physiological Dormancy

Abstract Key message Malus sylvestris(L.) Mill.,Pyrus communis(L.),Sorbus aucuparia(L.),Prunus avium(L.),Prunus padus(L.), andCornus sanguinea(L.) are related, co-occurring species producingorthodoxseeds. However, we observed differences in their response to storage conditions, such as storage at different seed moisture contents (5%, 8%, and 11%) and/or temperatures (− 3 °C, − 18 °C, and − 196 °C). Severe desiccation to ca. 5% of MC negatively affected seeds ofM. sylvestris. Seeds ofP. aviumwere sensitive to storage in LN or at − 18 °C.S. aucupariaseeds are best stored at − 3 °C, whereasC. sanguineaseeds tolerate desiccation and storage in LN. In general, species with deeper physiological dormancy (S. aucuparia,P. padus, andC. sanguinea) tended to be more tolerant to desiccation and low temperatures. For all species, storage conditions did not affect seedling growth. Context Wild fruit woody species face many threats such as genetic loss, population fragmentation, and alien species; thus, their genetic variability should be preserved. Aims To examine the effect of storage conditions on seed viability and the initial growth of seedlings of six European wild fruit species: Malus sylvestris (L.) Mill., Pyrus communis (L.), Sorbus aucuparia (L.), Prunus avium (L.), Prunus padus (L.), and Cornus sanguinea (L.). Methods Seeds were desiccated to three different levels of moisture content (ca. 5, 8, or 11%) and stored at three different temperatures (− 3°, − 18°, or − 196 °C; liquid nitrogen, LN) for up to 3 years. Germination and seedling emergence tests were performed as well as measurement of seedling growth. Results Desiccation of M. sylvestris seeds from 10.7 to 4.9% significantly lowered germination from 91 to 77% and seedling emergence from 88 to 74%. In P. avium, LN storage significantly inhibited seedling emergence, both in the laboratory and the greenhouse, but did not affect total seed germination. In P. communis, P. padus, and C. sanguinea, neither germination nor seedling emergence was affected by seed storage conditions. There were small or no differences in stem height and root collar diameter in the first year of seedling growth of stored seeds. Conclusion Species with deeper physiological dormancy (S. aucuparia, P. padus, C. sanguinea) tended to be more tolerant of various storage conditions. Seeds of P. padus and C. sanguinea can be stored long term at − 18 °C or in LN at 5–8% MC without losing viability. M. sylvestris and P. avium seeds are sensitive to desiccation below 6% MC or low temperature of storage at − 18 °C or − 196 °C, respectively. We observed that storage conditions had significant influence on germination and seedling emergence but had no effect on seedling growth after the first growing season.

scholarly journals Adjustment of the methodology of the tetrazolium test for estimating viability of Eugenia uniflora L. seeds during storage

2014 ◽  
Vol 36 (3) ◽  
pp. 344-351 ◽  
Author(s):  
Daiana Karoline Kaiser ◽  
Laura Cristiane Nascimento de Freitas ◽  
Rubia Priscila Biron ◽  
Simone Cristina Simonato ◽  
Michele Fernanda Bortolini
Keyword(s):  
Seed Viability ◽  
Germination Test ◽  
Preparation Methods ◽  
Eugenia Uniflora ◽  
Paper Towel ◽  
Plastic Bags ◽  
Longitudinal Cutting ◽  
Direct Immersion ◽  
Tetrazolium Test

The study aimed to adjust the methodology of the tetrazolium test to estimate seed viability of Eugenia uniflora L. (Surinam Cherry). Seeds were collected in September 2012 and divided into four lots: freshly harvested (Lot I); stored in plastic bags in a refrigerator at 10 °C for: 15 days (Lot II); 30 days (Lot III); and 45 days (Lot IV). The freshly harvested seeds were preconditioned with direct immersion in water and wet paper towel, followed or not by longitudinal cutting. The seeds were immersed in a 0.1% tetrazolium solution for 4 hours at 30 °C. Appropriate soaking and preparation methods were applied to the seeds lots using three tetrazolium concentrations: 0.1; 0.5; and 1.0%; and four preconditioning periods (2, 4, 6 and 8 hours) at 30 °C. The viability results obtained by the tetrazolium test were compared with those of the germination test. Direct immersion of seeds in water for 24 hours at 25 °C, followed by a longitudinal cut was efficient for preconditioning the seeds. Seed staining with tetrazolium solution at a concentration of 0.5% for 2 hours at 30 °C can be used to estimate the viability of freshly harvested and stored Surinam Cherry seeds.

scholarly journals Adaptation of the tetrazolium test method for estimating the viability of sorghum seeds

2014 ◽  
Vol 36 (2) ◽  
pp. 246-252 ◽  
Author(s):  
Tereza Cristina de Carvalho ◽  
Camila Ribeiro de Souza Grzybowski ◽  
Osvaldo de Castro Ohlson ◽  
Maristela Panobianco
Keyword(s):  
Quality Control ◽  
Filter Paper ◽  
Seed Quality ◽  
Seed Viability ◽  
Test Method ◽  
Accurate Assessment ◽  
Efficient Procedure ◽  
Paper Towels ◽  
Direct Immersion ◽  
Tetrazolium Test

The tetrazolium test is part of internal programs of seed quality control because it allows a quick, reliable and accurate assessment of seed viability. The purpose of this study was to determine, among the various methodologies suggested in the literature, a practical and efficient procedure for carrying out the tetrazolium test in sorghum seeds, with a view toward a rapid estimate of their viability. Four seed lots of the simple hybrid Buster were used, testing two forms of seed pre-conditioning (direct immersion in water and between moistened paper towels); two types of preparation (longitudinal cut of the seed through the embryo with immersion of one of the halves in the tetrazolium solution or placement of the two halves on filter paper moistened with tetrazolium solution); two forms of staining (on paper and with direct immersion in the solution) and three concentrations of tetrazolium solution (0.1%, 0.5% and 1.0%). The tetrazolium test may be efficiently conducted for sorghum seeds through pre-conditioning between paper towels for 18 hours at 20 ºC, with immersion staining of one half of the seed in tetrazolium solution at 0.1% for three hours at 40 ºC.

scholarly journals Assessment of physiological potential of stored pea (Pisum sativum L.) seeds

2013 ◽  
Vol 35 (1) ◽  
pp. 42-50 ◽  
Author(s):  
Fábio Mielezrski ◽  
Julio Marcos-Filho
Keyword(s):  
Electrical Conductivity ◽  
Growth Habit ◽  
Seedling Emergence ◽  
Accelerated Aging ◽  
Seed Storage ◽  
Seed Vigor ◽  
Seedling Length ◽  
Pea Seeds ◽  
Cold Chamber ◽  
Pea Seed

Research on pea seed storage and the identification of efficient seed vigor tests for this species is still insufficient, especially for cultivars that produce wrinkled seeds used for consumption in natura. The objective of this study was to verify the accuracy of tests to assess the physiological potential of pea seed lots stored in different environments. Four seed lots of two cultivars, 'Telefone Alta' (indeterminate growth habit) and 'Itapuã' (determinate growth habit) were stored under different environmental conditions for 8 months: a) laboratory uncontrolled b) dry and cold chamber (10 ºC and 30% RH), c) controlled environment (20 ºC and 70% RH). Seed vigor (accelerated aging, electrical conductivity, seedling length and seedling emergence) was evaluated initially and after four and eight months storage. Results showed that the use of seed lots of high initial physiological potential is fundamental for guaranteeing the desired performance of pea seeds during storage. Wrinkled pea seeds should be stored between harvest and sowing in an environment in which the sum of the relative humidity (%) and temperature (°C) does not exceed 70. Physiological potential of pea seeds should be evaluated by at least two vigor tests; consideration to accelerated aging and electrical conductivity tests is recommended.

scholarly journals Potential Role of Sequential Glufosinate and Foramsulfuron Applications for Management of Fescues (Festucaspp.) in Wild Blueberry

2017 ◽  
Vol 31 (1) ◽  
pp. 100-110 ◽  
Author(s):  
Scott N. White ◽  
Shanthanu Krishna Kumar
Keyword(s):  
Field Study ◽  
Seed Production ◽  
Potential Role ◽  
Seedling Recruitment ◽  
Seedling Emergence ◽  
Seed Viability ◽  
Early Spring ◽  
Seedling Mortality ◽  
Wild Blueberry

Sheep and hair fescue are perennial, tuft forming grasses that spread by seed and form dense sods in wild blueberry fields. These sods compete with the crop for resources and hinder harvest. Field and greenhouse studies were conducted in 2015 to evaluate 1) the effect of sequential glufosinate and foramsulfuron applications on suppression of fescues in the greenhouse and field, and 2) efficacy of glufosinate and foramsulfuron on fescue seedlings when applied at 2, 4, 6, and 8 wk after seedling emergence in the greenhouse. Glufosinate applications at 750 and 1,005 g ai ha−1followed by foramsulfuron application at 35 g ai ha−1reduced fescue leaf number and biomass relative to foramsulfuron application alone in the greenhouse. In the field study, fescue flowering tuft density, tuft inflorescence height, seed production, and seed viability were reduced by foramsulfuron alone, but there was a trend towards lower seed production and tuft height when fescues were treated with glufosinate at 1,005 g ha−1followed by foramsulfuron. Foramsulfuron caused low seedling mortality at all application timings evaluated, but glufosinate caused >90% mortality in seedlings when applied at 2, 4, 6, or 8 wk after seedling emergence. Our results suggest that sequential applications of these herbicides are less effective under field conditions relative to results obtained in the greenhouse, though burndown glufosinate applications may have a role in reducing fescue seedling recruitment. Additional research should be conducted to determine the effect of early spring and autumn glufosinate applications on fescue seedling recruitment and suppression of established fescue tufts with subsequent foramsulfuron applications.

scholarly journals Biological features of seeds of a wild-growing species of Poterium polygamum

2022 ◽  
Vol 354 (11-12) ◽  
pp. 97-99
Author(s):  
M. A. Starostina ◽  
N. G. Lapenko
Keyword(s):  
Natural Population ◽  
Seed Viability ◽  
Storage Period ◽  
Seed Storage ◽  
Scientific Basis ◽  
The Third ◽  
Biological Features ◽  
High Germination ◽  
Grass Stand ◽  
The Subject

Relevance. Biological features (morphometric indicators, viability and germinating power) of Poterium polygamum seeds found in natural communites of the Stavropol Territory have been studied. The conservation of wild-growing plant species in the natural population and the rational use of their potential are relevant.Methods. Years of study — 2013–2021. The subject of the study is Poterium polygamum seeds. Seeds were collected in 2013. Studies were carried out according to the methods of studying seed material. Freshly harvested seeds and seeds of different shelf life were germinated in petri dishes at a temperature of 18–20 °C.Results. The collected seeds of Poterium polygamum are quite large. The length of the seeds is 4–5 mm, the width is about 2.5 mm. The mass of 1000 pieces of seeds on average was 10.59 grams. Complete absence of viability of freshly harvested Poterium polygamum seeds was revealed. After three months, seed viability was 97%. This is a shallow physiological rest associated with the post-harvest maturation period. During the storage of seeds, their high germination was established — from 70 to 100%. The duration of seed storage is 8 years. The largest number of germinated seeds and their germinating power were noted in the third year of their storage (100%). Seeds germinated on the third or fourth day. The differences between the viability of seeds and their germinating power are not great. In the eighth year of storage of Poterium polygamum seeds, viability and germinating power were slightly reduced. The storage period of seeds, in which they retain the ability to germinate and the germinating power, is long. It has been found that the wild species Poterium polygamum belongs to plants with a high (70 to 100%) viability. This species is regularly resumed in the natural population. The revealed biological features of Poterium polygamum seeds can become the scientific basis during the reconstruction of degraded natural grass stand and in selection work.

scholarly journals Mid storage seed hardening: a mechanical method to maintain seed viability during long term jute seed preservation

2021 ◽  
Vol 8 (1) ◽  
pp. 79-88
Author(s):  
Md. Nasir Uddin ◽  
S. M. Mahbub Ali ◽  
Md. Abu Sadat ◽  
Md Amazed Hossain Chowdhury ◽  
Israt Jahan Mumu ◽  
...  
Keyword(s):  
Crop Production ◽  
Agricultural Sector ◽  
Seed Viability ◽  
Early Period ◽  
Chemical Properties ◽  
Seed Storage ◽  
Germination Percentage ◽  
Oil Degradation ◽  
Hardening Treatment

Seed plays an important role in agricultural sector for both production and consumption purpose. Availability of vigour seed is one of the major constraints for maximizing crop production. However, healthy seed can also lose its viability during seed storage by changing different physio-chemical properties. Influence of environmental factors and seed containers during storage leading to seed deterioration. In this research, mid storage seed hardening treatment was applied in different aged seeds of jute species (C. Capsularis & C. olitorius) with two types of storage bags. Seed hardening treatment showed the less moisture content with better germination percentage compared to the untreated species of jute seeds. Seed packing in polythene bags during both short and long term seed storages had higher viable seeds compared to the cloth packing seeds. The effect of seed hardening treatment on seed oil content and pattern of oil degradation is distinct in early period of storage. The faster rate of oil degradation, soluble protein and free amino acids was found in seeds of un-treated stored seeds in cloth bag. Contrary, very slow rate of oil degradation was observed in harden seed and stored in polythene bag which indicated better storability of harden seeds.

Seed storage and germination studies of Garcinia talbotii Raizada ex Santapau seeds - an endemic tree from Western Ghats of India

2016 ◽  
Vol 23 (2) ◽  
pp. 73-78
Author(s):  
T. Sabu ◽  
P.S. Shameer ◽  
Chitra Rajeswary ◽  
N. Mohanan ◽  
C. Anilkumar
Keyword(s):  
Ex Situ Conservation ◽  
Storage Temperature ◽  
Seed Viability ◽  
Seed Storage ◽  
Ex Situ ◽  
Hermetic Storage ◽  
Western Ghats Of India ◽  
Relationship Of ◽  
The Relationship ◽  
And Storage

Seeds of Garcinia talbotii remained viable hardly for two weeks in open room conditions. As part of ex-situ conservation of Garcinia talbotii, seeds longevity was studied since seeds are the main propagule. For this, the relationship of seed viability with respect to different moisture content and storage temperature were analyzed. Seed storage behaviour is also investigated. Being recalcitrant, seeds are desiccation sensitive as well as chilling sensitive. During hermetic storage of seeds at 300C /70 % RH seeds retained viability about 6 months.

High seed viability recorded in an endangered endemic species, 'Isoplexis isabelliana' (Scrophulariaceae), after more than 30 years of storage in a conservation seed bank

2021 ◽  
Vol 42 ◽  
pp. e69341
Author(s):  
Miguel Ángel González Pérez ◽  
Nereida Cabrera-García ◽  
Isabel Cayon-Fernández
Keyword(s):  
Seed Bank ◽  
Germination Rate ◽  
Genetic Material ◽  
Natural Populations ◽  
Seed Viability ◽  
Seed Storage ◽  
Botanical Garden ◽  
Storage Conditions ◽  
Seed Banks ◽  
Ex Situ

Conservation seed banks are essential for ex-situ conservation of genetic biodiversity. These institutions are especially relevant for threatened species and play a vital role in their conservation by preserving genetic material. However, samples deposited in the seed banks must germinate when necessary to use them (i.e., recovery plans, etc.). This study uses four accessions of the endemic endangered species from Gran Canaria Island (Canary Islands), Isoplexis isabelliana (Webb & Berthel.) Masf. (Scrophulariaceae). Germination tests were carried out to measure seed viability through time and the possible impact of seed storage on their viability. These accessions have been kept in the seed bank for four months to thirty years under different storage conditions. Germination results differed for seeds after 45 days of exposition using 16 hours light and 8 hours darkness at 17 °C. Accessions kept in the seed bank, independently of storage, showed a high germination percentage (89%). Whereas the accessions with rough storage conditions showed a 0% germination rate. The results highlighted the good state of conservation of the material deposited in the Seed Bank of the Botanical Garden "Viera y Clavijo" and the reliability of the temperature and humidity conditions in which the seeds of I. isabelliana have been stored. We consider these results as momentous since several natural populations of I. isabelliana has been affected by the last forest fire on the island.

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