scholarly journals Ultrasound Determination of Gestational Age Using Placental Thickness in Female Dogs: An Experimental Study

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
André Luiz Louzada Maldonado ◽  
Edward Araujo Júnior ◽  
Débora Sartori Mendonça ◽  
Luciano Marcondes Machado Nardozza ◽  
Antonio Fernandes Moron ◽  
...  

Objective. To verify if the placental thickness allows determining the gestational age, evaluating the correlation between the referred gestational age with the studied one, and the accuracy of the placental thickness measurement (biometry) with fetal morphologic parameters in bitches.Methods. The placental thickness of 336 bitches of diverse breeds was evaluated. Bitches were divided in three groups by body weight: small, medium, and big large size. The gestations pregnancies were evaluated by ultrasound from the third week of gestation. An analysis was performed between the mean values of the gestational age obtained of placental thickness by adjustment of curves and the reported gestational age. Student'st-test was applied to compare the mean of reported and placental thickness gestational age. Significance was defined asP<0.05.Results. A positive and statistically significant correlation exists between the placental thickness and gestational age. The expression that presents the best correlation coefficient and explanation was thickness of placenta = 0.021xgestational age −0.314.Conclusion. It is possible to determine the gestational age in relation to the placental thickness measured by ultrasound in bitches with a satisfactory accuracy in relation to fetal morphologic parameters as gestational vesicle, ribs, or kidneys.

2012 ◽  
Vol 69 (9) ◽  
pp. 765-770
Author(s):  
Sanja Knezevic ◽  
Nadezda Stojanovic ◽  
Ana Oros ◽  
Jasmina Knezevic

Background/Aim. Retinopathy of prematurity (ROP) is a multifactorial disease in premature infants. The aim of this study was to determine the incidence of ROP in children treated at the Center of Neonatology, Pediatric Clinic, Clinical Center in Kragujevac, Serbia. Methods. The study covered all children with birth weight below 2,000 g and/or gestational age below the 37th week, who from June 2006 to December 2009 underwent ophthalmological examination for ROP. The results of fundoscopy were classified in accordance with the International Classification of ROP. The treatment of infants and those with ROP was conducted in accordance with the early treatment of ROP study recommendations. We analyzed gestational age, birth weight and postconceptional age in two groups: healthy infants and those with severe form of ROP. Statistical analysis was performed using the SPSS 16. Results. A total of 478 children met the criteria of screening for ROP. Severe stage of ROP, which required laser treatment, had 102 (21.3%) children. Out of the infants with severe ROP 14 (13.7%) of the infants with APD had aggressive posterior disease, while two (0.4%) remained blind. The differences in the mean values of gestational age between the healthy and the children with severe form of the disease were statistically significant (p < 0.0005). The mean value of gestational age for the healthy children was 33.33 ? 2.28 weeks and for the seek infants 30.66 ? 2.79 weeks. The mean value of the weight in healthy children was 1.981 ? 407 g, and in sick children 1.535 ? 434 g which was statistically significant (p < 0.0005). Multivariate binary logistic regression showed that the occurrence of the disease depends on body weight and gestational age. Conclusion. The incidence of severe forms of ROP was 21.3%. Aggressive form of ROP was present in 13.7% of the children. The cut-off value for body weight was 1.740 g, and for gestational age 32.5 weeks.


1968 ◽  
Vol 51 (4) ◽  
pp. 828-834
Author(s):  
Omer Pelletier

Abstract The AOAC method and the Pelletier and Campbell method for the assay of niacinamide in multivitamin preparations were compared in two collaborative studies. In the first study, three multivitamin sirup preparations were analyzed by six laboratories; the proposed method gave higher results for two preparations but lower results for the third. In the second study, four types of multivitamin preparations were analyzed; twelve laboratories reported on the chemical methods and three laboratories on the microbiological method. There was no difference in the mean values for two types of samples, but one sample of multivitamin tablets yielded mean values about 5% lower by the proposed method, suggesting the presence of a trace of niacin; with a multivitamin and mineral sirup the proposed method gave values about 6% higher than the AOAC method and 3% lower than the microbiological assay. The coefficient of variation for any sample in any laboratory by the AOAC method was about 14% in the first study and 1 1% in the second study; by the proposed method it was 4 and 9% in the same respective studies. It is recommended that the present AOAC method be modified by adjusting the pH of sample hydrolysates in the range of 2.5—4.5 instead of 7—9 to reduce the absorhance of the blanks, and that the method of Pelletier and Campbell be adopted as official, first action.


The examination of a long series of observations in the search for periodicities by harmonic analysis is a tedious process. The method outlined below, although rough, has the merit of being much more rapid in its application, and has been found useful in the preliminary exploration of long meteorological records of periodic or quasi-periodic components. It is also useful in the study of periodicities of intermittent recurrence or variable length. The basis of the method is the division of a series of observations into series which are nearly, but not quite, equal, to half the length of the suspected periodicity. If each section were exactly half the periodicity the first, third, fifth, etc., sections would all occur in the same position relatively to the periodic curve, while the second, fourth, sixth, etc., would occur a half-cycle away. If each section is slightly less than half the length of the periodicity, then the third and so on, until after a certain interval the n th section occurs at a similar phase to the first. The number of sections required to bring this about depends on the relation between their length and the length of the periodicity. If the sections selected had been slightly longer than half the periodicity, the phase would advance somewhat between the first and third sections, and so on until the cycle was repeated. Thus by plotting the mean values of all the odd sections and drawing a curve through them we could obtain an idea of the length of the underlying periodicity. The even sections could be plotted in the same way, and if the series of observations were completely be the reverse of each other. Thus by substituting for the mean value of each section its difference from the mean of the whole series, and reversing the sign of all the even sections, we can bring the latter into line with the odd sections and draw a single curve representing all the observations.


Author(s):  
E.A. Derkach , O.I. Guseva

Objectives: to compare the accuracy of equations F.P. Hadlock and computer programs by V.N. Demidov in determining gestational age and fetal weight in the third trimester of gestation. Materials: 328 patients in terms 36–42 weeks of gestation are examined. Ultrasonography was performed in 0–5 days prior to childbirth. Results: it is established that the average mistake in determination of term of pregnancy when using the equation of F.P. Hadlock made 12,5 days, the computer program of V.N. Demidov – 4,4 days (distinction 2,8 times). The mistake within 4 days, when using the equation of F.P. Hadlock has met on average in 23,1 % of observations, the computer program of V.N. Demidov — 65,9 % (difference in 2,9 times). The mistake more than 10 days, took place respectively in 51,7 and 8,2 % (distinction by 6,3 times). At a comparative assessment of size of a mistake in determination of fetal mass it is established that when using the equation of F.P. Hadlock it has averaged 281,0 g, at application of the computer program of V.N. Demidov — 182,5 g (distinction of 54 %). The small mistake in the mass of a fetus which isn't exceeding 200 g at application of the equation of F.P. Hadlock has met in 48,1 % of cases and the computer program of V.N. Demidov — 64,0 % (distinction of 33,1 %). The mistake exceeding 500 g has been stated in 18 % (F.P. Hadlock) and 4,3 % (V.N. Demidov) respectively (distinction 4,2 times). Conclusions: the computer program of V.N. Demidov has high precision in determination of term of a gestation and mass of a fetus in the III pregnancy.


Author(s):  
J Grøndahl-HANSEN ◽  
N Agerlin ◽  
L S Nielsen ◽  
K Danø

An enzyme-linked immunosorbent assay (ELISA) was developed for the measurement of human urokinase-type plasminogen activator (u-PA) in plasma and serum. Microtiter plates were coated with a monoclonal antibody and incubated with standard or sample. Bound u-PA was quantitated with polyclonal antibodies conjugated with biotin, followed by avidin-peroxidase. The assay was 10-fold as sensitive as other previously reported ELISAs, the detection limit being approximately 1 pg of u-PA in a volume of 100 μl with a linear dose-response up to 15 pg of u-PA. The assay detected active u-PA and its inactive proenzyme form equally well and the recovery of both forms was higher than 90% in plasma. A variety of structurally related proteins, including t-PA, were tested, but no reaction with proteins other than u-PA and its amino-terminal degradation product were observed. The intra-assay and inter-assay coefficients of variation for determination of u-PA in plasma were 7.6% and 8.4%, respectively. The assay was equally applicable to serum. The values obtained with plasma and serum were similar, and the results were not affected by small variations in the preparation of the samples. The ELISA was used to measure the concentration of u-PA in plasma from 34 healthy donors. The mean values for u-PA in plasma from healthy donors was 1.1 ng/ml ± 0.3 ng/ml (SD) (range 0.6 - 1.5 ng/ml). No significant differences were found between men and women and no correlation between u-PA concentration and age could be demonstrated.The mean u-PA concentration in plasma from healthy donors obtained in this study is substantially lower than that reported by others. This might be due to different methods of determination of the protein content of the standard preparations or to differences in the specificity of the assays.


2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Leila Shafie Bafti ◽  
Maryam Alsadat Hashemipour ◽  
Hamidreza Poureslami ◽  
Zeinab Hoseinian

The aim of the present study was to evaluate the relationship between BMI and tooth decay in a population of Iranian children. In this cross-sectional descriptive/analytical study, 1482 children were selected from kindergartens and preschool centers in Kerman, Iran. The children underwent examination of deciduous teeth (using the dmft index) after determination of height and weight for calculation of BMI. The relationship between BMI (after adjustment for age) and dmft was determined using Poisson’s regression model. The mean of dmft in children with normal BMI was 1.5-fold that in subjects with extra body weight. Age had a significant effect on dmft. In addition, dmft was higher in boys compared to girls. The results of the present study showed that caries rate in the deciduous teeth of 3–6-year-old children decreases with an increase in body weight.


2016 ◽  
Vol 46 (6) ◽  
pp. 1014-1018 ◽  
Author(s):  
Charline Zaratin Alves ◽  
Lennis Afraire Rodrigues ◽  
Carlos Henrique Queiroz Rego ◽  
Josué Bispo da Silva

ABSTRACT: Crambe is a rapeseed with high oil content and can be used as a winter cover or as a source of raw material for the production of biodiesel, however espite the growing interest in the culture, research on the subject is still incipient, especially concerning the seed production and analysis technology. The purpose of this study is to evaluate the physiological quality of crambe seeds, 'FMS Brilhante' cultivar, by testing the pH of exudate. Five seed lots were submitted to the determination of water content and the tests of germination and vigor (first count, emergence and tetrazolium). In the conduction of pH exudate test, temperatures (25 and 30oC), and periods of seed imbibition in water (15, 30 and 45 minutes) were tested. The experiment was conducted in a completely randomized manner, with four replicates, and the mean values were compared by the Tukey test at 5% probability; Pearson correlation between the pH of the exudate and initial tests was also made. Testing the pH of exudate is promising for separating lots of crambe seeds and the following combinations of 25°C/30 minutes or 30°C/45 minutes can be used.


2002 ◽  
Vol 54 (3-4) ◽  
pp. 69-74 ◽  
Author(s):  
Svetlana Savin-Zegarac ◽  
Dubravka Cvejic ◽  
Olgica Nedic ◽  
R. Radosavljevic ◽  
Ivana Petrovic

A few years after the iodine content of salt in Serbia was increased from 7 to 15 mg/kg NaCI, iodine, thyroxine (T4) and triiodothyronine (T3) concentrations were measured in thyroid tissue obtained at autopsy from 21 human neonates who died within 30 days after birth. The thyroidal iodine as well as T4 and T3 content per gland in?creased progressively with gestational age of human neonates (r = 0.73, 0.70 and 0.67 respectively, p < 0.001). In seven newborns (gestational age 36 to 41 weeks) the mean values for total iodine, T4 and T3 per gland were 109.1 ?g, 52.2 ?g and 4.4 ?g respectively. The results of iodine and iodothyroninc content found in neonatal thyroid gland, particularly at the end of gestation and a few days of postnatal life, indicates that the iodine supply was satisfactory.


1980 ◽  
Vol 43 (3) ◽  
pp. 393-402 ◽  
Author(s):  
Suzanne Large ◽  
G. Neal ◽  
J. Glover ◽  
O. Thanangkul ◽  
R. E. Olson

1. Changes in total retinol-binding protein (RBP), the holoprotein (holoRBP) and prealbumin (PA) concentrations have been monitored in plasma of thirty protein- and vitamin A-deficient preschool children from within a few hours up to 7 weeks after treatment with retinol and a good-quality protein diet.2. The children were classified into groups according to nutritional status as having either kwashiorkor, marasmus-kwashiorkor or marasmus, and given formula diets whose protein and energy contents increased stepwise from 1 g and 105 kJ/kg body-weight respectively up to 4 g and 733 kJ/kg body-weight after 4 weeks. Retinol was administered in the forms of retinyl palmitate either orally or intramuscularly.3. PA and total RBP were determined by electroimmunoassay procedures and the holoRBP by its fluorescence after separation from other plasma proteins.4. RBP in plasma of the vitamin A-deficient child is largely denatured and incapable of binding administered retinol, which must first be taken up by the liver before native holoRBP is released. An increased pool of native apoprotein accumulates in the liver during vitamin A deficiency which is released into plasma quickly after retinol uptake to form peak concentrations of total and holoRBP approximately 3 h after dosing intramuscularly and 6 h orally.5. The accumulated pool of RBP was highest in livers from the marasmus group and lowest in those from the kwashiorkor group, reflecting their relative capacities to synthesize plasma proteins.6. The mean plasma concentrations of total and holoRBP for the various groups were minimal 24–48 h after dosing with retinol and then improved almost linearly over the following week.7. Mean plasma PA concentrations of the various groups on admission were also in order of the severity of their malnutrition. There was little or no change in this protein concentration over the first 24 h after dosing with retinol, but thereafter the mean values rose almost linearly over 2 weeks. Albumin on the other hand changed little during the first week. The results show that PA is the more sensitive measurement of protein nutritional status.


2021 ◽  
pp. 64-66
Author(s):  
Sambhunath Bandyopadhyay ◽  
Ritayan Sasmal ◽  
Debarshi Jana

Accurate gestational age determination is very important for management of continuation and termination planning of the pregnancy. rd To establish placental thickness as a parameter for determination of gestational age and fetal growth pattern at 3 trimester. 100 primigravida women who are fullling the inclusion criteria and attend gynae &obs OPD or admitted in their third trimester at IPGME&R from January 2017 to june 2018. Placental thickness at 3rd trimester USG scan is moderately correlated with gestational age, if placental thickness expressed in millimeter then it correlated with gestational age at weeks. rd st Placental thickness with >3.2 cm (32 mm) at 3 trimester almost associated with good fetal outcome, with APGAR score >8 at 1 min and birth weight >2500 gm.


Sign in / Sign up

Export Citation Format

Share Document