scholarly journals Association of IL10-1082 and IFN-γ+874 Polymorphisms with Cervical Cancer among Tunisian Women

ISRN Genetics ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
Sabrina Zidi ◽  
Yosra Benothmen ◽  
Ikram Sghaier ◽  
Ezzeddine Ghazoueni ◽  
Amel Mezlini ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Study Design ◽  
Protective Factor ◽  
Healthy Controls ◽  
Exact Test ◽  
Healthy Women ◽  
Arms Pcr ◽  
Il10 Promoter ◽  
Ifn Γ

Objective. The aim of this study was to investigate the role of IL10-1082 and IFN-γ+874 polymorphisms in susceptibility to cervical cancer among Tunisian women. Study Design. The IL10-1082 and IFN-γ+874 polymorphisms were analyzed by ARMS-PCR in 160 healthy women and 122 with cervical cancer. The search for associations between those polymorphisms and cervical cancer was based on the χ2 test or Fisher's exact test. Results. The IFN-γ+874 polymorphism showed significant increased frequency of T allele in healthy controls compared with patients (OR = 0.71, 95% CI = 0.50–1.01, and P=0.0459) and individuals with homozygote IFN-γ+874 T/T genotype were at lesser risk of cervical cancer (OR = 0.53, 95% CI = 0.31–0.92, and P=0.015). However, carriers of allele have higher risk for developing cervical cancer (OR = 1.88, 95% CI = 1.09–3.24, and P=0.015). At the polymorphic nucleotide in position 1082 of the IL10 promoter, no differences were found between patients and controls subjects. Conclusion. Our study shows that the T/T genotype polymorphism of IFN-γ+874 T>A is a protective factor for cervical cancer among Tunisian women.

scholarly journals Association of cervical carcinogenesis risk with HPV16 E6 and E7 variants in the Taizhou area, China

BMC Cancer ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Mei-Zhen Dai ◽  
Yi Qiu ◽  
Xing-Hong Di ◽  
Wei-Wu Shi ◽  
Hui-Hui Xu
Keyword(s):  
Cervical Cancer ◽  
Cervical Carcinogenesis ◽  
Chi Square ◽  
Major Health Problem ◽  
Hpv16 E6 ◽  
Exact Test ◽  
Chi Square Test ◽  
E6 And E7

Abstract Background Human papillomavirus (HPV) type 16 accounts for a larger share of cervical cancer and has been a major health problem worldwide for decades. The progression of initial infection to cervical cancer has been linked to viral sequence properties; however, the role of HPV16 variants in the risk of cervical carcinogenesis, especially with longitudinal follow-up, is not fully understood in China. Methods We aimed to investigate the genetic variability of HPV16 E6 and E7 oncogenes in isolates from cervical exfoliated cells. Between December 2012 and December 2014, a total of 310 single HPV16-positive samples were selected from women living in the Taizhou area, China. Sequences of all E6 and E7 oncogenes were analysed by PCR-sequencing assay. Detailed sequence comparison, genetic heterogeneity analyses and maximum-likelihood phylogenetic tree construction were performed with BioEdit Sequence Alignment Editor and MEGA X software. Data for cytology tests and histological diagnoses were obtained from our Taizhou Area Study with longitudinal follow-up for at least 5 years. The relationship between HPV16 variants and cervical carcinogenesis risk was analysed by the chi-square test or Fisher’s exact test. Results In this study, we obtained 64 distinct variation patterns with the accession GenBank numbers MT681266-MT681329. Phylogenetic analysis revealed that 98.3% of HPV16 variants belong to lineage A, in which the A4 (Asian) sublineage was dominant (64.8%), followed by A2 (12.1%), A1 (11.4%), and A3 (10.0%). The A4 (Asian) sublineage had a higher risk of CIN2+ than the A1–3 (European) sublineages (OR = 2.69, 95% CI = 1.04–6.97, P < 0.05). Furthermore, nucleotide variation in HPV16 E6 T178G is associated with the development of cervical cancer. Conclusion These data could provide novel insights into the role of HPV16 variants in cervical carcinogenesis risk in China.

scholarly journals PVT1 Assumes Signifying Capacity in Cervical Cancer Diagnosis.

2020 ◽  
Author(s):  
Shuangqing Cao ◽  
Lei Zheng
Keyword(s):  
Cervical Cancer ◽  
Roc Curve ◽  
Mrna Level ◽  
Hpv Infection ◽  
Clinical Effects ◽  
Healthy Controls ◽  
Operating Characteristics ◽  
Large Tumor ◽  
Diagnostic Role

Abstract Background: Cervical cancer (CC) is the second most prevalent malignancy among women, which severely threatens patients’ health. The study was conducted to determine the diagnostic role of plasmacytoma variant translocation 1 (PVT1) in CC to improve patients outcomes.Methods: The qRT-PCR was used to determine the expression level of PVT1 mRNA in CC samples and healthy controls. Chi-square test was used to determine the clinical effects of patients’ features on PVT1 expression. The receiver operating characteristics (ROC) curve with the area under the curve (AUC) was used as a tool for assessing the diagnostic role of PVT1 expression in CC.Results: The PVT1 mRNA level was significantly higher in CC samples than healthy controls (P<0.0001). Large tumor size (P=0.006), positive uterus infiltration (P=0.031) and advanced FIGO stages (P=0.011) were contributed to the elevated expression of PVT1 level. However, there was no close relationship between PVT1 expression and other clinical parameters, including age (P=0.205), family history (P=0.073), positive HPV infection (P=0.155 and histological type (P=0.159). The ROC curve showed the optimal cutoff point for PVT1 was 2.325, providing the sensitivity and specificity of 85.84% and 72.15%, respectively. Moreover, the AUC was 0.856, suggesting PVT1 level could be regarded as a diagnostic biomarker in CC (P<0.0001, 95%CI= 0.803-0.909).Conclusion: In summary, the level of PVT1 mRNA was significantly increased in CC samples and the up-regulation of PVT1 could distinguish CC patients from healthy controls.

scholarly journals ILC1s and ILC3s Exhibit Inflammatory Phenotype in Periodontal Ligament of Periodontitis Patients

2021 ◽  
Vol 12 ◽  
Author(s):  
Changyi Li ◽  
Jianyue Liu ◽  
Jie Pan ◽  
Yuhui Wang ◽  
Lei Shen ◽  
...  
Keyword(s):  
Immune Responses ◽  
Periodontal Ligament ◽  
Inflammatory Diseases ◽  
Innate Lymphoid Cells ◽  
Lymphoid Cells ◽  
Mucosal Barrier ◽  
Healthy Controls ◽  
Inflammatory Phenotype ◽  
Ifn Γ

Innate lymphoid cells (ILCs) are emerging as important players in inflammatory diseases. The oral mucosal barrier harbors all ILC subsets, but how these cells regulate the immune responses in periodontal ligament tissue during periodontitis remains undefined. Here, we show that total ILCs are markedly increased in periodontal ligament of periodontitis patients compared with healthy controls. Among them, ILC1s and ILC3s, particularly NKp44+ILC3 subset, are the predominant subsets accumulated in the periodontal ligament. Remarkably, ILC1s and ILC3s from periodontitis patients produce more IL-17A and IFN-γ than that from healthy controls. Collectively, our results highlight the role of ILCs in regulating oral immunity and periodontal ligament inflammation and provide insights into targeting ILCs for the treatment of periodontitis.

scholarly journals Interleukin-35 Prevents Development of Autoimmune Diabetes Possibly by Maintaining the Phenotype of Regulatory B Cells

2021 ◽  
Vol 22 (23) ◽  
pp. 12988
Author(s):  
Zhengkang Luo ◽  
Sara Lundin ◽  
Mariela Mejia-Cordova ◽  
Imane Hassani ◽  
Martin Blixt ◽  
...  
Keyword(s):  
B Cells ◽  
Low Dose ◽  
Autoimmune Diabetes ◽  
Healthy Controls ◽  
Regulatory B Cells ◽  
Experimental Autoimmune Uveitis ◽  
Ifn Γ ◽  
Experimental Autoimmune

The anti-inflammatory role of regulatory B cells (Breg cells) has been associated with IL-35 based on studies of experimental autoimmune uveitis and encephalitis. The role of Breg cells and IL-35+ Breg cells for type 1 diabetes (T1D) remains to be investigated. We studied PBMCs from T1D subjects and healthy controls (HC) and found lowered proportions of Breg cells and IL-35+ Breg cells in T1D. To elucidate the role of Breg cells, the lymphoid organs of two mouse models of T1D were examined. Lower proportions of Breg cells and IL-35+ Breg cells were found in the animal models of T1D compared with control mice. In addition, the systemic administration of recombinant mouse IL-35 prevented hyperglycemia after multiple low dose streptozotocin (MLDSTZ) injections and increased the proportions of Breg cells and IL-35+ Breg cells. A higher proportion of IFN-γ+ cells among Breg cells were found in the PBMCs of the T1D subjects. In the MLDSTZ mice, IL-35 administration decreased the proportions of IFN-γ+ cells among the Breg cells. Our data illustrate that Breg cells may play an important role in the development of T1D and that IL-35 treatment prevents the development of hyperglycemia by maintaining the phenotype of the Breg cells under an experimental T1D condition.

scholarly journals Association of interleukin 2, interleukin 12, and interferon-γ with intervertebral disc degeneration in Iranian population

2020 ◽  
Author(s):  
Sara Hanaei ◽  
Sina Abdollahzade ◽  
Maryam Sadr ◽  
Mohammad Hossein Mirbolouk ◽  
Ehsan Fattahi ◽  
...  
Keyword(s):  
Intervertebral Disc ◽  
Disc Degeneration ◽  
Intervertebral Disc Degeneration ◽  
Interleukin 2 ◽  
Interleukin 12 ◽  
Healthy Controls ◽  
Eligibility Criteria ◽  
Age Related ◽  
Ifn Γ

Abstract Background: Intervertebral disc degeneration (IVDD) is an age-related degenerative disease, presenting with low back pain or radicular pain. The inflammatory changes would occur in discs in the process of IVDD. Therefore, the inflammatory and anti-inflammatory cytokines, as well as their respective genes, have been proposed to play roles in pathophysiology of disease. This study has been conducted to elucidate the role of IL-2, IL-12, and IFN-γ single nucleotide polymorphisms (SNP) in this disease.Method: 76 patients who were diagnosed with IVDD and 140 healthy controls who complied with eligibility criteria were included. A total volume of 5cc peripheral blood was obtained from each participant to investigate the IL-2 +166G/T, IL-2 -330G/T, IL-12 -1188A/C, and IFN-γ +847A/T SNPs through PCR-SSP method.Results: The ‘TG’ and ‘TT’ genotypes of IL-2 -330G/T polymorphism were significantly more common among patients and healthy controls respectively. The ‘GT’ and ‘TT’ haplotypes of IL-2 (comprised of -330G/T, and +166G/T SNPs) were also more common among patients and controls respectively.Conclusion: This study indicated the significant role of IL-2 genotypes and haplotypes in IVDD. These SNPs were differently distributed in patients and controls. Therefore, alteration in the structure of IL-2 gene could play an important role in pathophysiology of IVDD.

scholarly journals Activated but impaired IFN-γ production of mucosal-associated invariant T cells in patients with hepatocellular carcinoma

2021 ◽  
Vol 9 (11) ◽  
pp. e003685
Author(s):  
Wenyong Huang ◽  
Dongmei Ye ◽  
Wenjing He ◽  
Xiaoshun He ◽  
Xiaomin Shi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
T Cells ◽  
Peripheral Blood ◽  
Healthy Controls ◽  
Activation Markers ◽  
Mait Cells ◽  
Hla Dr ◽  
Ifn Γ ◽  
And Function

ObjectiveMucosal-associated invariant T (MAIT) cells are innate T cells with immunoregulatory activity and were recently found to be associated with various tumor types. The role of intrasinusoidal MAIT cells in hepatocellular carcinoma (HCC) has not been fully characterized.DesignPeripheral blood samples were obtained from patients with HCC and healthy controls. Liver-associated mononuclear cells (LMCs) were collected from liver perfusions of donors and patients with HCC undergoing liver transplantation. Blood and liver perfusates from patients with HCC were analyzed by flow cytometry for CD3 +CD161+Vα7.2+MAIT cell frequency, phenotype, and function.ResultsThere were fewer MAIT cells in the peripheral blood and liver of patients with HCC than in the healthy controls. Interferon-γ (IFN-γ) production by these cells was also reduced. Peripheral MAIT cells showed upregulation of HLA-DR (Human Leukocyte Antigen DR） and the inhibitory molecule PD-1 (Programmed Cell Death Protein 1), but no significant differences in upregulation were found in intrasinusoidal MAIT cells. MAIT cells were significantly enriched in the liver relative to that in the peripheral blood of patients with HCC. High levels of activation markers and exhaustion markers including HLA-DR, CD69, and PD-1 were observed in LMCs of patients with HCC but not in the peripheral blood. Single-cell RNA sequencing revealed that intrasinusoidal MAIT cells exhibited distinct features in patients with HCC and the controls.ConclusionOur study showed that alterations in MAIT cells are associated with HCC. The distinct activity and function of MAIT cells in the peripheral blood and liver of patients with HCC might suggest a potential role of these cells in disease pathogenesis.

Serum YKL-40 levels in patients with cervical cancer are elevated compared to patients with cervical intraepithelial neoplasia and healthy controls

2006 ◽  
Vol 24 (18_suppl) ◽  
pp. 5047-5047 ◽  
Author(s):  
J. S. Johansen ◽  
A. Roslind ◽  
C. Palle ◽  
I. J. Christensen ◽  
H. J. Nielsen ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cervical Intraepithelial Neoplasia ◽  
Elevated Serum ◽  
Intraepithelial Neoplasia ◽  
Healthy Controls ◽  
Serum Samples ◽  
Healthy Women ◽  
Stage Ib ◽  
Exact Function ◽  
Stage Ia

5047 Background: YKL-40 is secreted by cancer cells, macrophages and neutrophils. The exact function of YKL-40 is unknown. It may be a growth or differentiation factor, play a role in angiogenesis or protect against apoptosis. High serum YKL-40 is associated with poor prognosis in breast-, colorectal-, ovarian-, prostate-, small cell lung cancer and malignant melanoma. The aim was to examine serum YKL-40 in patients with cervical cancer and cervical intraepithelial neoplasia (CIN). Methods: YKL-40 was determined by ELISA (Quidel, Santa Clara, CA) in pretreatment serum samples from 116 patients with cervical cancer (FIGO stage Ia (N = 5), Ib (N = 55), II (N = 27), III (N = 25) and IV (N = 4); median age 50, range 27–87 years) and 152 patients with cervical CIN (34 years, 20–80 years). The controls included 63 healthy women undergoing sterilization (36 years, 25–59 years) and a group of 134 healthy women (51 years, 18–79 years). Results: Serum YKL-40 was increased (p < 0.001) in the patients with cervical cancer (median 76 μg/l, range 20–2310) compared to patients with CIN (45 μg/l, 20–288), women undergoing sterilization (37 μg/l, 20–125) and healthy women (43 μg/l, 20–172). Patients with CIN had slightly elevated serum YKL-40 levels. Serum YKL-40 increased with increasing stage (Ia: median 36 μg/l, range 26–137; Ib: 56 μg/l, 20–984; II: 92 μg/l, 28–2310; III: 163 μg/l, 40–1474; and IV: 224 μg/l, 137–391; p < 0.001 Kruskal-Wallis test). All stage IV patients, 72% stage III, 52% stage II, 24% stage Ib, 40% stage Ia and 13% of patients with CIN had elevated serum YKL-40 compared to healthy controls (95th percentile). Patients with squamous cell carcinoma had higher serum YKL-40 (median 92 μg/l, range 27–2310, N = 86) compared to patients with adenosquamous (67 μg/l, 20–93, N = 9, p = 0.027) and adenocarcinoma (43 μg/l, 20–1472, N = 20, p < 0.001). Conclusion: Median serum YKL-40 is elevated in patients with stage Ib - IV cervical cancer. The serum level of YKL-40 seems to be correlated to the stage. No significant financial relationships to disclose.

scholarly journals Acute-Phase Levels of CXCL8 as Risk Factor for Chronic Arthralgia Following Chikungunya Virus Infection

2021 ◽  
Vol 12 ◽  
Author(s):  
Leile Camila Jacob-Nascimento ◽  
Caroline Xavier Carvalho ◽  
Monaíse Madalena Oliveira Silva ◽  
Mariana Kikuti ◽  
Rosângela Oliveira Anjos ◽  
...  
Keyword(s):  
Acute Phase ◽  
Joint Pain ◽  
Chikungunya Virus ◽  
Healthy Controls ◽  
Sample Collection ◽  
Chikv Infection ◽  
Flow Cytometric ◽  
Multivariable Analyses ◽  
Ifn Γ

The immunopathogenesis of chikungunya virus (CHIKV) infection and the role of acute-phase immune response on joint pain persistence is not fully understood. We investigated the profile of serum chemokine and cytokine in CHIKV-infected patients with acute disease, compared the levels of these biomarkers to those of patients with other acute febrile diseases (OAFD) and healthy controls (HC), and evaluated their role as predictors of chronic arthralgia development. Chemokines and cytokines were measured by flow Cytometric Bead Array. Patients with CHIKV infection were further categorized according to duration of arthralgia (≤ 3 months vs &gt;3 months), presence of anti-CHIKV IgM at acute-phase sample, and number of days of symptoms at sample collection (1 vs 2-3 vs ≥4). Patients with acute CHIKV infection had significantly higher levels of CXCL8, CCL2, CXCL9, CCL5, CXCL10, IL-1β, IL-6, IL-12, and IL-10 as compared to HC. CCL2, CCL5, and CXCL10 levels were also significantly higher in patients with CHIKV infection compared to patients with OAFD. Patients whose arthralgia lasted &gt; 3 months had increased CXCL8 levels compared to patients whose arthralgia did not (p&lt;0.05). Multivariable analyses further indicated that high levels of CXCL8 and female sex were associated with arthralgia lasting &gt;3 months. Patients with chikungunya and OAFD had similar cytokine kinetics for IL-1β, IL-12, TNF, IFN-γ, IL-2, and IL-4, although the levels were lower for CHIKV patients. This study suggests that chemokines may have an important role in the immunopathogenesis of chronic chikungunya-related arthralgia.

scholarly journals Multiple Sclerosis: Shall We Target CD33?

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1334
Author(s):  
Vasileios Siokas ◽  
Zisis Tsouris ◽  
Athina-Maria Aloizou ◽  
Christos Bakirtzis ◽  
Ioannis Liampas ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Central Nervous System ◽  
Nervous System ◽  
Protective Factor ◽  
Statistical Significance ◽  
Healthy Controls ◽  
Reference Allele ◽  
Myeloid Lineage ◽  
The Central Nervous System

Background: Multiple sclerosis (MS) is a chronic disease of the central nervous system (CNS). Myeloid lineage cells (microglia and macrophages) may participate in the pathogenic mechanisms leading to MS. CD33 is a transmembrane receptor, mainly expressed by myeloid lineage cells. CD33 rs3865444 is a promoter variant previously associated with Alzheimer’s disease, whose role in MS remains obscure. Objective: To assess the role of CD33 rs3865444 in MS risk. Methods: We genotyped 1396 patients with MS and 400 healthy controls for the presence of the CD33 rs3865444 variant. Odds ratios (ORs) with the respective 95% confidence intervals (CIs), were calculated with the SNPStats software, assuming five genetic models (co-dominant, dominant, recessive, over-dominant, and log-additive), with the G allele as the reference allele. The value of 0.05 was set as the threshold for statistical significance. Results: CD33 rs3865444 was associated with MS risk in the dominant (GG vs. GT + TT; OR (95% C.I.) = 0.79 (0.63–0.99), p = 0.041) and the over-dominant (GG + TT vs. GT; OR (95% C.I.) = 0.77 (0.61–0.97), p = 0.03) modes of inheritance. Given that the GG genotype was more frequent and the GT genotype was less frequent in MS patients compared to controls—while the observed frequency of the TT genotype did not differ between the two groups—the observed difference in MS risk may be stemming from either the GG (as a risk factor) or the GT (as a protective factor) genotype of CD33 rs3865444. Conclusions: Our preliminary results suggest a possible contribution of CD33 rs3865444 to MS. Therefore, larger multiethnic studies should be conducted, investigating the role of CD33 rs3865444 in MS.

scholarly journals HUBUNGAN PERAN BIDAN DENGAN PERILAKU PERIKSA INSPEKSI VISUAL ASAM ASETAT (IVA)

2019 ◽  
Vol 11 (01) ◽  
pp. 96
Author(s):  
Triani Yuliastanti ◽  
Rismawati .
Keyword(s):  
Cervical Cancer ◽  
Sampling Technique ◽  
Developed Countries ◽  
P Value ◽  
Fisher Exact Test ◽  
Test Results ◽  
Chi Square ◽  
Cross Sectional ◽  
Exact Test

ABSTRAK                           Latar Belakang: Kanker serviks merupakan salah satu penyakit kanker yang paling banyak terjadi pada kaum wanita. Kanker serviks menduduki urutan tertinggi di negara berkembang dan urutan ke 10 di negara maju atau urutan ke 5 secara global Berdasar pada kebijakan pemerintah mengadakan program baru pencegahan Ca Serviks yang dilakukan melalui pemeriksaan Inspeksi Visual Asam Asetat (IVA). Tujuan : Mengetahui hubungan peran bidan dengan perilaku periksa Inspeksi Visual Asam Asetat (IVA). Metode: Desain penelitian menggunakan korelasional dengan pendekatan cross sectional. populasi penelitian semua WUS di Desa Karanggeneng Kecamatan Boyolali sebanyak  971 WUS. Berdasarkan rumus slovin didapatkan jumlah sempel yaitu 91 responden. Teknik sampling accidental sampling. Alat pengumpulan data berupa kuesioner. Analisa data menggunakan uji chi square. Hasil : Penelitian menunjukkan responden yang diberikan penyuluhan dan  melakukan pemeperiksaan  IVA  sebanyak  41,2%, responden yang pernah dilakukan penyuluhan namun tidak periksa IVA sebanyak 58,8%. Sedangkan 100% responden yang tidak mendapatkan penyuluhan tidak melakukan pemeriksaan IVA. Hasil uji fisher exact didapatkan nilai p 0,006  < =0,05 sehingga ada hubungan antara peran bidan dengan perilaku periksa IVA. Kesimpulan : Ada hubungan  antara  peran bidan dengan perilaku periksa IVA. Bidan diharapkan melakukan penyuluhan dan memotivasi ibu secara berulang-ulang tentang pemeriksaan IVA agar meningkatkan pengetahuan dan minat ibu melakukan pemeriksaan IVA sebagai deteksi dini kangker servikKata kunci:  Peran bidan, perilaku periksa IVABIDAN ROLE RELATIONSHIP ROLE OF MIDWIVES WITH CHECK BEHAVIOR OF VISUAL ACID ACID INSPECTION (IVA) ABSTRACTBackground: Cervical cancer is one of the most common cancers in women. Cervical cancer ranks highest in developing countries and ranks 10th in developed countries or 5th place globally. Based on government policy, the implementation of a new program to prevent Cervical Ca is done by examining Visual Acetate Acid inspection (IVA). Objective: To determine the relationship between the role of midwives and the behavior of check for Visual Acetate Acid inspection (IVA). Method: The study design used correlational with cross sectional approach. the research population of all WUS in Karanggeneng Village, Boyolali District was 971 WUS. Based on the Slovin formula, the number of respondents was 91 respondents. The sampling technique is accidental sampling. Data collection tool in the form of a questionnaire. Data analysis using chi square test. Results: The study showed that respondents who were given counseling and conducted IVA examinations were 41.2%, respondents who had been given counseling did not check IVA for 58.8%. While 100% of respondents who did not receive counseling did not carry out IVA examinations. Fisher exact test results obtained p value 0.006 <= 0.05 so that there was a relationship between the role of midwives and IVA check behavior. Conclusion: There is a relationship between the role of midwives and the behavior of check IVA. Midwives are expected to conduct counseling and motivate mothers repeatedly about IVA examination so as to increase knowledge and interest in mothers conducting IVA examination as early detection of cervical cancerKeywords: Role of midwives, IVA check behavior.

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