scholarly journals Antifungal Effect of Lavender Essential Oil (Lavandula angustifolia) and Clotrimazole onCandida albicans: AnIn VitroStudy

Scientifica ◽  
2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Fereshteh Behmanesh ◽  
Hajar Pasha ◽  
Ali Asghar Sefidgar ◽  
Mohsen Taghizadeh ◽  
Ali Akbar Moghadamnia ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Essential Oil ◽  
Cell Count ◽  
Vaginal Discharge ◽  
Pairwise Comparison ◽  
Vaginal Candidiasis ◽  
Fungal Cell ◽  
Antifungal Effect ◽  
Significant Difference

Background. The treatment of candidiasis infections is an important problem in the health care system. This study aimed to investigate thein vitroeffect of lavender essential oil and clotrimazole on isolatedC. albicansfrom vaginal candidiasis.Materials and Methods. In this clinical trial,C. albicansisolated from the vaginal discharge samples was obtained.Results. The pairwise comparison showed that lavender and clotrimazole had a significant difference; this difference in the lavender group was lower than clotrimazole. But, after 48 hours, there was no difference seen between groups. There was a significant difference between clotrimazole and DMSO groups. Comparing the changes between groups based on the same dilution, at 24 h and 48 h in clotrimazole group, showed a significant difference two times in the fungal cell count that its average during 48 h was less than 24 h. A significant difference was observed between the two periods in lavender group, only at the dilutions of 1/20 and 1/80. The average fungal cell count after 48 h was also lower in lavender group.Conclusions. Given that the lavender has antifungal activity, this can be used as an antifungal agent. However, more clinical studies are necessary to validate its use in candida infection.

scholarly journals Comparison of Shear Bond Strength and Microleakage of Various Bulk-fill Bioactive Dentin substitutes: An in vitro Study

2016 ◽  
Vol 17 (12) ◽  
pp. 997-1002 ◽  
Author(s):  
Fahad I Alkhudhairy ◽  
Zeeshan H Ahmad
Keyword(s):  
Bond Strength ◽  
Shear Bond Strength ◽  
Physical And Mechanical Properties ◽  
Pairwise Comparison ◽  
In Vitro Study ◽  
Composite Resins ◽  
Intergroup Comparison ◽  
Significant Difference ◽  
Tukey Honestly Significant Difference

ABSTRACT Introduction Various bulk-fill materials depending on their composition, viscosity, and flow ability have different physical and mechanical properties. The aim of this in vitro study was to determine and compare the shear bond strength and microleakage properties of activa restorative with other bulk-fill restorative materials surefil (SDR), Biodentine, ever X posterior. Materials and methods Forty permanent premolars were selected for shear bond strength, and 20 permanent premolars were selected with class II cavities on mesial and distal side for microleakage. Universal testing device was used to assess the shear bond strength. Microleakage was checked using dye penetration method under a stereomicroscope. Mean and standard deviation values were calculated from the recorded values. Intergroup comparison was done by one-way analysis of variance (ANOVA) followed by pairwise comparison using Tukey honestly significant difference (HSD) post hoc test. Results The mean shear bond strength was highest for SDR surefil followed by Ever X posterior, Bioactive restorative, and Biodentine respectively. In this study, SDR (surefil) showed better shear bond strength and better microleakage properties compared with the other test materials (F = 186.7157, p < 0.05). Conclusion The result of this study showed that flowable and fiber-reinforced composites have better shear bond strength and microleakage properties. Clinical significance Flowable bulk-fill composite resins can be used as dentin substitutes because of its superior properties. How to cite this article Alkhudhairy FI, Ahmad ZH. Comparison of Shear Bond Strength and Microleakage of Various Bulk-fill Bioactive Dentin substitutes: An in vitro study. J Contemp Dent Pract 2016;17(12):997-1002.

Vaginal suppositories of cumin seeds essential oil for treatment of vaginal candidiasis: Formulation, in vitro, in vivo, and clinical evaluation

2021 ◽  
Vol 157 ◽  
pp. 105602
Author(s):  
Noura H. Abd Ellah ◽  
Asmaa S. Shaltout ◽  
Shreen M.M. Abd El Aziz ◽  
Ahmed M. Abbas ◽  
Hasnaa G. Abd El Moneem ◽  
...  
Keyword(s):  
Essential Oil ◽  
Clinical Evaluation ◽  
Vaginal Candidiasis

scholarly journals Efficacy and Safety Curcuma zadoaria L. to Inactivate the Hydatid Cyst Protoscoleces

2020 ◽  
Vol 15 (1) ◽  
pp. 64-71 ◽  
Author(s):  
Hossein Mahmoudvand ◽  
Mahbobeh Pakravanan ◽  
Farnaz Kheirandish ◽  
Sareh Jahanbakhsh ◽  
Maryam Sepahvand ◽  
...  
Keyword(s):  
Body Weight ◽  
Essential Oil ◽  
Hydatid Cyst ◽  
Intraperitoneal Injection ◽  
Ex Vivo ◽  
Clinical Chemistry ◽  
Hematological Parameters ◽  
Efficacy And Safety ◽  
Significant Difference

Background: The present work aimed to evaluate the chemical composition of Curcuma zadoaria essential oil and to investigate its efficacy and safety against hydatid cyst protoscoleces. Methods: Collected protoscoleces from liver fertile hydatid cysts of infected sheep were exposed to different concentrations of the essential oil (75, 150, 300 μl/mL) for 5-30 min in vitro and ex vivo. Then, by using the eosin exclusion assay, the viability of protoscoleces was studied. In the next step, 24 male NMRI mice were examined to assess the toxicity of C. zadoaria essential oil by measuring the biochemical and hematological parameters. Results: Based on the obtained results, the LD50 value of intraperitoneal injection of the C. zadoaria essential oil was 1.76 mL/kg of body weight and the maximum non-fatal dose was 0.96 mL/kg of body weight. C. zadoaria essential oil had a strong proto scolicidal activity in vitro so that at the 300 and 150 μl/ml entirely eliminates the parasite after 5 and 10 minutes; whereas, weak proto scolicidal activity was observed at lower doses. Ex vivo assay, no similar effect with in vitro was observed, therefore, more time is required to show a potent proto scolicidal activity. C. zadoaria essential oil at the concentrations of 300 and 150 μl/mL after an exposure time of 7 and 12 min, killed 100% of protoscoleces within the hydatid cyst, respectively. After intraperitoneal injection of the C. zadoaria essential oil for 2 weeks, no significant difference (p > 0.05) was observed in the clinical chemistry and hematologic parameters at the doses of 0.15, 0.3, 0.6 mL/kg. Conclusion: The obtained results in vitro and ex vivo exhibited that C. zadoaria essential oil had a favorable proto scolicidal activity on hydatid cyst protoscoleces. However, more supplementary works are required to verify these findings by assessing clinical subjects.

Microcatheter delivery of neurotherapeutics: compatibility with mesenchymal stem cells

2020 ◽  
Vol 133 (4) ◽  
pp. 1182-1190 ◽  
Author(s):  
Visish M. Srinivasan ◽  
Joy Gumin ◽  
Kevin M. Camstra ◽  
Stephen R. Chen ◽  
Jeremiah N. Johnson ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Cell Count ◽  
Glioma Cells ◽  
Injection Velocity ◽  
Viral Agent ◽  
Intraarterial Infusion ◽  
Significant Difference

OBJECTIVEBone marrow–derived human mesenchymal stem cells (BM-hMSCs) have been used in clinical trials for the treatment of several neurological disorders. MSCs have been explored as a delivery modality for targeted viral therapeutic agents in the treatment of intracranial pathologies. Delta-24-RGD, a tumor-selective oncolytic adenovirus designed to target malignant glioma cells, has been shown to be effective in animal models and in a recent clinical trial. However, the most efficient strategy for delivering oncolytic therapies remains unclear. BM-hMSCs have been shown to home toward glioma xenografts after intracarotid delivery. The feasibility of selective intraarterial infusion of BM-hMSCs loaded with Delta-24-RGD (BM-hMSC-Delta-24) to deliver the virus to the tumor is being investigated. To evaluate the feasibility of endovascular intraarterial delivery, the authors tested in vitro the compatibility of BM-hMSC-Delta-24 with a variety of commercially available, clinically common microcatheters.METHODSBM-hMSCs were cultured, transfected with Delta-24-RGD, and resuspended in 1% human serum albumin. The solution was then injected via 4 common neuroendovascular microcatheters of different inner diameters (Marathon, Echelon-14, Marksman, and SL-10). Cell count and viability after injection through the microcatheters were assessed, including tests of injection velocity and catheter configuration. Transwell assays were performed with the injected cells to test the efficacy of BM-hMSC-Delta-24 activity against U87 glioma cells. BM-hMSC-Delta-24 compatibility was also tested with common neuroendovascular medications: Omnipaque, verapamil, and heparin.RESULTSThe preinfusion BM-hMSC-Delta-24 cell count was 1.2 × 105 cells/ml, with 98.7% viability. There was no significant difference in postinfusion cell count or viability for any of the catheters. Increasing the injection velocity from 1.0 ml/min to 73.2 ml/min, or modifying the catheter shape from straight to tortuous, did not significantly reduce cell count or viability. Cell count and viability remained stable for up to 5 hours when the cell solution was stored on ice. Mixing BM-hMSC-Delta-24 with clinical concentrations of Omnipaque, verapamil, and heparin prior to infusion did not alter cell count or viability. Transwell experiments demonstrated that the antiglioma activity of BM-hMSC-Delta-24 was maintained after infusion.CONCLUSIONSBM-hMSC-Delta-24 is compatible with a wide variety of microcatheters and medications commonly used in neuroendovascular therapy. Stem cell viability and viral agent activity do not appear to be affected by catheter configuration or injection velocity. Commercially available microcatheters can be used to deliver stem cell neurotherapeutics via intraarterial routes.

Compare the effect of aromatherapy using lavender and Damask rose essential oils on the level of anxiety and severity of pain following C-section: A double-blinded randomized clinical trial

2020 ◽  
Vol 17 (3) ◽  
Author(s):  
Ali Abbasijahromi ◽  
Hamed Hojati ◽  
Saeid Nikooei ◽  
Hossein Kargar Jahromi ◽  
Hamid Reza Dowlatkhah ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Essential Oil ◽  
Essential Oils ◽  
Randomized Clinical Trial ◽  
Control Group ◽  
Content Type ◽  
Gate Control Theory ◽  
Significant Difference ◽  
The Mean ◽  
Damask Rose

AbstractBackgroundAnxiety is the most common psychological reaction in women during labor. Similar to numerous other surgeries, postoperative pain is also reported following cesarean section (C-section). According to the (Gate) Control Theory, there is a relationship between pain and psychological problems such as anxiety. Accordingly, the present study aimed to compare the effect of aromatherapy using lavender and Damask rose essential oils on the level of anxiety and severity of pain following C-section.MethodsThis triple-group randomized clinical trial was performed on 90 mothers who visited Motahari Hospital of Jahrom, Iran, for C-section in 2017. The incidence and severity of pain and anxiety were measured and recorded for all three groups prior to intervention. The intervention groups underwent aromatherapy with lavender and Damask rose essential oils. Patients were asked to inhale cotton balls, separately stained with three drops of each essential oil at a distance of 10 cm for 30 mins. The severity of pain and anxiety was measured using the visual analogue scale (VAS) and the Spielberger State-Trait Anxiety Inventory (STAI) 5 min after the specified process, respectively. The control group underwent aromatherapy in a similar fashion with normal saline. Finally, data were analyzed using descriptive statistical indices and ANOVA and Kruskal–Wallis tests in SPSS 21.ResultsThere was no significant difference between the three groups in the mean severity of pain and anxiety before the intervention (p>0.05). The mean severity of pain and overt anxiety in the lavender and Damask rose aromatherapy groups was significantly different than the control group after the intervention (p<0.001). In addition, no significant difference was observed between the overt and overall anxiety levels of the two intervention groups after the intervention (p>0.05).ConclusionsThe findings suggested that inhalation aromatherapy can reduce the severity of overt anxiety and pain after C-section, with Damask rose essential oil showing a larger effect than lavender.

scholarly journals The antifungal effect of roselle calyx extract on Trichophyton rubrum growth in vitro

2012 ◽  
Vol 10 (1) ◽  
pp. 17-22
Author(s):  
SITA AULIA SARI ◽  
RUBEN DHARMAWAN ◽  
PARAMASARI DIRGAHAYU
Keyword(s):  
Trichophyton Rubrum ◽  
Positive Control ◽  
Negative Control ◽  
Antifungal Effect ◽  
Inhibition Effect ◽  
Extract Concentration ◽  
Significant Difference ◽  
Object Of Study ◽  
One Way Anova

Sari SA, Dharmawan R, Dirgahayu P. 2012. The antifungal effect of roselle calyx extract on Trichophyton rubrum growth in vitro. Biofarmasi 10: 17-22. Dermatophytosis is a fungal infection on skin that one of them caused by Trichophyton rubrum. Dermatophytosis treatment by using chemical drugs has many shortcomings, such as a high cost and a drug resistance. Roselle (Hibiscus sabdariffa L.) calyx content was flavonoid, which have an antifungal effect. Flavonoids on roselle calyx include anthocyanin, gossypeptin (hexahydroxyflavone) 3-glucoside, flavonol glucoside hibiscritin, flavonoid gossypeptin, delphinidine 3-monoglucoside, cyanidin 3-monoglucoside. The objective of this study was to determine the effect of roselle calyx on Trichophyton rubrum growth in vitro. The study was performed as an experimental laboratory. The object of study was T. rubrum. The sample of T. rubrum colonies in this study was taken by a random sampling. The study used T. rubrum colonies on seven Sabouraud Dextrose Agar plates. Each plate had four holes. Each hole was filled by aquadest as a negative control, fluconazole 25 µg/mL as a positive control, and various roselle calyx extract concentrations (10%, 20%, 30%, 40% and 50%). The plates were incubated in an incubator with a temperature of 25oC for 7 days and measured for the diameter of roselle calyx extract inhibition effect. The data were collected and analyzed by One-way ANOVA and Least Significance Difference (LSD) tests on SPSS 16.0 for Windows. The result of One-way ANOVA test showed that there was a difference of inhibition diameter mean among all of the various roselle calyx extract concentration groups (p<0.05). The diameter of roselle calyx extract inhibition effect increased for each concentration up to 50%. The inhibition diameter of positive control compared to 20% roselle calyx extract concentration had no a significant difference. The study was concluded that roselle calyx extract has an antifungal effect to T. rubrum growth in vitro.

scholarly journals In Vitro Antimicrobial Activity of Frankincense Oils from Boswellia sacra Grown in Different Locations of the Dhofar Region (Oman)

Antibiotics ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 195 ◽  
Author(s):  
Vita Di Stefano ◽  
Domenico Schillaci ◽  
Maria Grazia Cusimano ◽  
Mohammed Rishan ◽  
Luay Rashan
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Volatile Components ◽  
Clinical Settings ◽  
Antifungal Effect ◽  
Microbial Infections ◽  
Grade 3 ◽  
The Comparative Study ◽  
Boswellia Sacra

Frankincense essential oils from Boswellia sacra have been commonly used to treat microbial infections from as early as the 11th century. The main feature of the plant is its gum resin, from which it is possible to obtain essential oils. In the present study, we focused on the comparative study of the oils extracted from the resins of three different Boswellia sacra cultivars (Najdi, Sahli and Houjri). From each of frankincense resin three successive essential oil samples (Grade 1, Grade 2, Grade 3) were obtained. Houjri gum resin gave the lowest percentage (5%) of total essential oil content but showed the maximum number of volatile components in all three grades. Najdi Grade 2 essential oil showed a minimum inhibitory concentration (MIC) of 52 mg/mL toward relevant pathogens Staphylococcus aureus and Pseudomonas aeruginosa, and samples from Grade 2 of Sahily and Houjiri were particularly active against a dermatological strain Propionibacterium acnes, displaying MIC values of 0.264 and 0.66 mg/mL, respectively. Data obtained from in vitro studies showed that all essential oils had a significant antifungal effect against Candida albicans and Malassezia furfur, showing MIC values ranging from 54.56 to 0.246 mg/mL. This work aims to increase the number of substances available in the fight against pathogens and to combat the phenomenon of antibiotic resistance, encouraging the use of alternative resources, especially in non-clinical settings (farms, food processing, etc.).

scholarly journals Identification of sulfur components enhancing the anti-Candida effect of Lactobacillus rhamnosus Lcr35

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Caroline Dausset ◽  
Stéphanie Bornes ◽  
Sylvie Miquel ◽  
Nathalie Kondjoyan ◽  
Magaly Angenieux ◽  
...  
Keyword(s):  
Sulfur Compound ◽  
Sodium Thiosulfate ◽  
Lactobacillus Rhamnosus ◽  
Active Metabolites ◽  
Fungal Cell ◽  
Functional Link ◽  
Antifungal Effect ◽  
Highly Active ◽  
First Time

Abstract GYNOPHILUS (Lcr REGENERANS) is a live biotherapeutic product (LBP) aimed at restoring the vaginal microbiome and contains the live biotherapeutic microorganism Lactobacillus rhamnosus Lcr35. In this study, the LBP formulation and manufacturing process significantly enhanced the anti-Candida activity of L. rhamnosus Lcr35, with a complete loss of viability of the yeast after 48 h of coincubation. Sodium thiosulfate (STS), one excipient of the product, was used as a potentiator of the anti-Candida spp. activity of Lactobacilli. This contact-independent phenomenon induced fungal cell disturbances, as observed by electron microscopy observations. Nonverbal sensory experiments showed clear odor dissimilarities between cocultures of L. rhamnosus Lcr35 and C. albicans in the presence and absence of STS, suggesting an impact of odor-active metabolites. A volatolomic approach allowed the identification of six odor-active compounds, including one sulfur compound that was identified as S-methyl thioacetate (MTA). MTA was associated with the antifungal effect of Lcr35, and its functional link was established in vitro. We show for the first time that the LBP GYNOPHILUS, which is a highly active product in the reduction of vulvovaginal candidiasis, requires the presence of a sulfur compound to fully achieve its antifungal effect.

scholarly journals Cephalometric comparison of obstructive sleep apnea patients and healthy controls

2013 ◽  
Vol 07 (01) ◽  
pp. 048-054 ◽  
Author(s):  
Ahmet Yalcin Gungor ◽  
Hakan Turkkahraman ◽  
H. Huseyin Yilmaz ◽  
Murat Yariktas
Keyword(s):  
Clinical Trial ◽  
Obstructive Sleep Apnea ◽  
Essential Oil ◽  
Placebo Control ◽  
Controlled Clinical Trial ◽  
Healthy Controls ◽  
Lippia Sidoides ◽  
Obstructive Sleep ◽  
Significant Difference ◽  
Gel Preparation

ABSTRACTObjective: This parallel controlled clinical trial evaluated the effect of a gel containing Lippia sidoides essential oil on plaque and gingivitis control.Methods: Thirty patients (n=30) were randomly selected and allocated into three groups: Lippia sidoides (LS, n=10), chlorhexidine (CLX, n=10) or placebo (control, n=10). Plaque and bleeding index were recorded at baseline and after three months. All volunteers were instructed to brush with the gel three times a day throughout the experiment period.Results: There was a significant reduction on plaque and gingivitis in the test groups (P<.05), but no statistically significant difference was observed between them (P<.05).Conclusion: A gel preparation containing 10% Lippia sidoides essential oil was an efficient herbal antiplaque and antigingivitis agent. (Eur J Dent 2013;7:28-34)

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