scholarly journals Aqueous Extract ofAgaricus blazeiMurrill Prevents Age-Related Changes in the Myenteric Plexus of the Jejunum in Rats

2015 ◽  
Vol 2015 ◽  
pp. 1-13
Author(s):  
Ana Paula de Santi-Rampazzo ◽  
João Paulo Ferreira Schoffen ◽  
Carla Possani Cirilo ◽  
Mariana Cristina Vicente Umada Zapater ◽  
Fernando Augusto Vicentini ◽  
...  
Keyword(s):  
Glial Cells ◽  
Cell Body ◽  
Aqueous Extract ◽  
Myenteric Plexus ◽  
Blood Parameters ◽  
Quantitative Morphology ◽  
Age Related ◽  
Intestinal Length ◽  
Daily Dose ◽  
Jejunal Wall

This study evaluated the effects of the supplementation with aqueous extract ofAgaricus blazeiMurrill (ABM) on biometric and blood parameters and quantitative morphology of the myenteric plexus and jejunal wall in aging Wistar rats. The animals were euthanized at 7 (C7), 12 (C12 and CA12), and 23 months of age (C23 and CA23). The CA12 and CA23 groups received a daily dose of ABM extract (26 mg/animal) via gavage, beginning at 7 months of age. A reduction in food intake was observed with aging, with increases in the Lee index, retroperitoneal fat, intestinal length, and levels of total cholesterol and total proteins. Aging led to a reduction of the total wall thickness, mucosa tunic, villus height, crypt depth, and number of goblet cells. In the myenteric plexus, aging quantitatively decreased the population of HuC/D+neuronal and S100+glial cells, with maintenance of the nNOS+nitrergic subpopulation and increase in the cell body area of these populations. Supplementation with the ABM extract preserved the myenteric plexus in old animals, in which no differences were detected in the density and cell body profile of neurons and glial cells in the CA12 and CA23 groups, compared with C7 group. The supplementation with the aqueous extract of ABM efficiently maintained myenteric plexus homeostasis, which positively influenced the physiology and prevented the death of the neurons and glial cells.

scholarly journals Age-related cytokine profile in uncomplicated Plasmodium malaria infection

2011 ◽  
pp. 323-327 ◽  
Author(s):  
Youtchou Mirabeau Tatfeng ◽  
Dennis Edo Agbonlahor
Keyword(s):  
Malaria Infection ◽  
Malaria Diagnosis ◽  
Blood Parameters ◽  
Enzyme Linked Immunosorbent Assay ◽  
Monocyte Count ◽  
Healthy Controls ◽  
Age Related ◽  
Cytokine Levels ◽  
Adolescents And Adults ◽  
The Mean

Objective: Malaria infection is severe in children who are believed to be more at risk because of their relative poor immunity against the disease. Some cytokine levels (IFN-g, IL-2, IL-4, and IL-10) of children, adolescents, and adults were assessed in this study. Methods: Cytokine levels were assayed by using Enzyme Linked Immunosorbent Assay (ELISA). Malaria diagnosis and blood parameters were carried out by using standard parasitological and haematological techniques. Results: The mean cytokine levels were significantly elevated in children, adolescent, and adult subjects when compared to their respective healthy controls (p<0.05). Also, mean IFN-g and IL-2 levels were significantly higher in children than in adults (IFN-g: 57.31±77.79 pg/ml vs. 20.37± 2.95 pg/ml, and IL-2: 108.75±63.53 pg/ml vs. 66.09±45.34 pg/ml) (p<0.05) and adolescents (IFN-g: 20.37± 2.95 pg/ml and IL-2: 66.09±45.34 pg/ml) respectively. Furthermore, mean IL-10 level was significantly lower in children (7.39±15.08 pg/ml) than mean level in adults (22.73±13.89 pg/ml). The mean haematological parameters revealed significant increase in total white blood cell, CD4, and CD8 count and significant decrease in the hematocrit of children in relation to adolescent and adult subjects (p<0.05). However, mean monocyte count was significantly higher in subjects than in their respective healthy controls (p<0.05). Conclusion: Findings in this study revealed better Th1 driven immune response in children than in adolescents and adults.

Age-related changes in the myenteric plexus of the porcine bowel

2009 ◽  
Vol 44 (9) ◽  
pp. 1771-1777 ◽  
Author(s):  
Thambipillai Sri Paran ◽  
Udo Rolle ◽  
Prem Puri
Keyword(s):  
Myenteric Plexus ◽  
Age Related ◽  
Age Related Changes

scholarly journals Behavioral and Hematological Response of Clarias gariepinus juvenile exposed to Acute concentrations of Aqueous Extract of Siam Weed (Chloromolaena odorata) leaf as Anesthetic agent

2017 ◽  
Author(s):  
Iheanacho Stanley Chidi ◽  
Nworu Shedrack
Keyword(s):  
Aqueous Extract ◽  
Exposure Time ◽  
Hematological Parameters ◽  
Clarias Gariepinus ◽  
Blood Parameters ◽  
Anesthetic Agent ◽  
Distilled Water ◽  
Chromolaena Odorata ◽  
Blood Samples ◽  
Significant Difference

AbstractThis experiment was carried out to investigate the effect of Siam Weed (chloromelena odorata) on the heamatology of Clarias gariepinus juvenile. A total of one hundred and fifty (150) juvenile of Clarias gariepinus were randomly assigned to different concentrations of C. odorata leave aqueous extract in a completely randomize design (CRD). The concentrations were 50mg/l, 100mg/l, 150mg/l, 200mg/l. Distilled water (0.00 mg/l) was used as the control. The fish exhibited stressful behavior which was higher as the concentration of Chromolaena odorata leave extract increased. There was a gradual decrease with time until a state of calmness, which was subsequently followed by death. The effect on 96hr exposed period was recorded and blood samples collected at 24hr and 96hr interval. Result on hematological parameters revealed significant difference (P<0.05) among treatments with increase in exposure time for all the blood parameters. C. odorata at increased concentrations affected the behavior and hematology of C. gariepinus.

scholarly journals Age-related clonal haematopoiesis is more prevalent in older adults with HIV: the ARCHIVE study

2020 ◽  
Author(s):  
Mark Dawson ◽  
Nila Dharan ◽  
Paul Yeh ◽  
Mark Bloch ◽  
Miriam Yeung ◽  
...  
Keyword(s):  
Hiv Infection ◽  
Age Groups ◽  
Selective Advantage ◽  
Blood Parameters ◽  
Hiv Positive ◽  
Hiv Status ◽  
Age Related ◽  
Potential Risk Factors ◽  
Infection And Inflammation ◽  
Hiv Negative

Abstract People with HIV have higher rates of certain comorbidities, particularly cardiovascular disease and some malignancies, than people without HIV. As somatic mutations associated with age related clonal haematopoiesis (CH) are linked to similar comorbidities in the general population, we hypothesized that CH may be more prevalent in people with HIV. To address this issue, we established a prospective cohort study recruiting 220 HIV-positive and 226 HIV negative participants aged 55 years or older in Australia. Demographic characteristics, clinical data and peripheral blood were collected to assess for the presence of CH mutations and identify potential risk factors for and clinical sequelae of CH. Investigators testing for CH were blinded to participants’ HIV status. In total, 132 CH mutations were identified in 99 (22.2%) of 446 participants. CH was more prevalent in HIV-positive participants than HIV-negative participants (27.7% vs. 16.8%, p =0.006), overall and across all age groups. HIV infection was associated with an increased odds of having CH (adjusted odds ratio 2.10, 95% confidence interval 1.30-3.38, p=0.002). The most common genes mutated were DNMT3A (48.5%), TET2(20.5%) and ASXL1 (11.4%). CH and HIV infection were independently associated with increases in blood parameters and biomarkers associated with inflammation. These data suggest a selective advantage for the emergence of CH in the context of chronic infection and inflammation related to HIV infection.

scholarly journals Neurons of the Myenteric Plexus of the Rat Ileum Are Vulnerable to the Action of the Herbicide 2,4-Dichlorophenoxyacetic Acid

2020 ◽  
Vol 9 (1) ◽  
pp. 14
Author(s):  
Ricardo De Melo Germano ◽  
Renata De Britto Mari ◽  
Paula Montanhini Favetta ◽  
Marcelo Alberto Elias ◽  
Wesley Alves Trindade ◽  
...  
Keyword(s):  
Cell Body ◽  
Myenteric Plexus ◽  
Daily Intake ◽  
Cholinergic Neurons ◽  
Dichlorophenoxyacetic Acid ◽  
Body Area ◽  
Rat Ileum ◽  
Male Wistar Rats ◽  
The Central Nervous System ◽  
2,4 Dichlorophenoxyacetic Acid

The herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) is widely used in several countries. Research on the neurotoxicity of 2,4-D has been focused on the central nervous system, and little is known about its effects on the myenteric plexus. Therefore, to elucidate the neurotoxicity of 2,4-D and the viability of its use, we investigated the effects of daily intake of 5 mg 2,4-D/kg for 60 days on the myenteric plexus neurons of the rat ileum using quantitative and morphometric analyses. Twenty male Wistar rats aged 60 days were divided into two groups (n=10 rats/group). The group E animals received daily 5 mg doses of 2,4-D/kg diluted in 1 mL of water, whereas the group C animals were not treated with the herbicide. The animals were euthanized with anesthetic after 60 days; subsequently, the ileum was dissected out, and procedures were performed to visualize the total population of neurons (Giemsa staining), the nitrergic neurons (NADPHd+), and the estimated population of cholinergic neurons (NADPHd-). The results were statistically analyzed and compared between groups. In group E, the densities of Giemsa-stained neurons and NADPHd- neurons decreased (p<0.05) by 7% and 10%, respectively, whereas the density of NADPHd+ neurons remained constant. The cell body area was 5.8% greater (p<0.05) for the NADPHd+ neurons but remained unchanged for the neurons stained with Giemsa. These results suggest that 2,4-D causes a reduction in neuronal density, particularly for cholinergic neurons, and promotes an increase in the cell body area of nitrergic neurons, leading to hypertrophy.

scholarly journals Development of Tolerance to Chronic Intermittent Furosemide Therapy in Pediatric Patients

2017 ◽  
Vol 22 (6) ◽  
pp. 394-398
Author(s):  
Gloria J. Kim ◽  
Edmund Capparelli ◽  
Gale Romanowski ◽  
James A. Proudfoot ◽  
Adriana H. Tremoulet
Keyword(s):  
Fluid Balance ◽  
Pediatric Patients ◽  
Daily Intake ◽  
Total Daily Dose ◽  
Day Range ◽  
Age Related ◽  
Days Of Therapy ◽  
Daily Dose ◽  
The Difference ◽  
Development Of Tolerance

OBJECTIVES The purpose of this study is to describe whether tolerance develops in pediatric patients receiving chronic intermittent furosemide therapy, to characterize when it occurs and whether age-related variations exist. The effects of increasing total daily dose of furosemide and concurrent diuretics and vasopressors were assessed as secondary aims. METHODS Charts from patients receiving intravenous or oral furosemide for at least 3 consecutive days of therapy between June 1, 2013, and December 31, 2013, were reviewed retrospectively. Daily net fluid balance was used as the objective marker for development of tolerance. Net fluid balance (mL/kg/mg) was defined as the difference in a patient's daily intake and urine output (mL), normalized by weight (kg) and total daily dose of furosemide (mg). RESULTS Sixty-one patients, aged 2 days to 20 years (median 3 years), were included in this study. Median daily dose of furosemide was 1.96 mg/kg/day (range, 0–13.7 mg/kg/day). Average net fluid balance for all patients on the first day and last day of therapy was 6.83 and 26.66 mL/kg/mg, respectively (p = 0.011). Linear regression and Spearman's correlation found no significant relationship between age and difference in net fluid balance between the first and last day. Linear mixed-effects model for net fluid balance with day as covariate found that net fluid balance increases over time (p = 0.002). CONCLUSIONS Pediatric patients appear to develop tolerance to chronic intermittent furosemide therapy.

scholarly journals Impaired Mitophagy in Neurons and Glial Cells during Aging and Age-Related Disorders

2021 ◽  
Vol 22 (19) ◽  
pp. 10251
Author(s):  
Vladimir Sukhorukov ◽  
Dmitry Voronkov ◽  
Tatiana Baranich ◽  
Natalia Mudzhiri ◽  
Alina Magnaeva ◽  
...  
Keyword(s):  
Glial Cells ◽  
Brain Aging ◽  
Cellular Level ◽  
Aging Brain ◽  
The Past ◽  
Age Related ◽  
Accumulation Of Damage ◽  
Quantity Control ◽  
The Brain

Aging is associated with a decline in cognitive function, which can partly be explained by the accumulation of damage to the brain cells over time. Neurons and glia undergo morphological and ultrastructure changes during aging. Over the past several years, it has become evident that at the cellular level, various hallmarks of an aging brain are closely related to mitophagy. The importance of mitochondria quality and quantity control through mitophagy is highlighted by the contribution that defects in mitochondria–autophagy crosstalk make to aging and age-related diseases. In this review, we analyze some of the more recent findings regarding the study of brain aging and neurodegeneration in the context of mitophagy. We discuss the data on the dynamics of selective autophagy in neurons and glial cells during aging and in the course of neurodegeneration, focusing on three mechanisms of mitophagy: non-receptor-mediated mitophagy, receptor-mediated mitophagy, and transcellular mitophagy. We review the role of mitophagy in neuronal/glial homeostasis and in the molecular pathogenesis of neurodegenerative disorders, such as Parkinson’s disease, Alzheimer’s disease, and other disorders. Common mechanisms of aging and neurodegeneration that are related to different mitophagy pathways provide a number of promising targets for potential therapeutic agents.

scholarly journals Hepatoprotective activity of aqueous extract from Caragana jubata (Pall.) Poir. shoots in the model of acute hepatitis induced by acetaminophen in rats

2018 ◽  
Vol 64 (3) ◽  
pp. 241-246 ◽  
Author(s):  
P.A. Kakorin ◽  
I.V. Babenkova ◽  
Y.O. Teselkin ◽  
G.V. Ramenskaya ◽  
T.A. Demura ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Alkaline Phosphatase ◽  
Aqueous Extract ◽  
Wistar Rats ◽  
Total Bilirubin ◽  
Histological Study ◽  
Blood Parameters ◽  
Hepatoprotective Activity ◽  
Reference Preparation ◽  
Liver Homogenates

The aim of the study was to investigate the hepatoprotective activity of an aqueous extract of Caragana jubata (Pall.) Poir. Acute experimental hepatitis was induced by acetaminophen administration of 1000 mg/kg. Studies were conducted in white Wistar rats. The aqueous extract of C. jubata demonstrated the hepatoprotective effect, comparable to that of the reference preparation “Carcil”. This was manifested by the normalization of biochemical blood parameters (ALT, AST, alkaline phosphatase, cholesterol, total bilirubin) and antioxidant activity of liver homogenates, determined by the method based on oxidation of luminol induced by 2,2¢-azo-bis-2-amidinopropane. Normalization of morphofunctional indices was also shown in a histological study of liver of rats that received aqueous extract from C. jubata.

scholarly journals Immunohistochemical and ultrastructural analysis of the maturing larval zebrafish enteric nervous system reveals the formation of a neuropil pattern

2019 ◽  
Author(s):  
Phillip A. Baker ◽  
Matthew D. Meyer ◽  
Ashley Tsang ◽  
Rosa A. Uribe
Keyword(s):  
Nervous System ◽  
Enteric Nervous System ◽  
Glial Cells ◽  
Myenteric Plexus ◽  
Larval Fish ◽  
Intestinal Barrier ◽  
Stem Cell Population ◽  
Functional Studies ◽  
Larval Stages ◽  
Enteric Ganglia

AbstractThe gastrointestinal tract is constructed with an intrinsic series of interconnected ganglia that span its entire length, called the enteric nervous system (ENS). The ENS exerts critical local reflex control over many essential gut functions; including peristalsis, water balance, hormone secretions and intestinal barrier homeostasis. ENS ganglia exist as a collection of neurons and glia that are arranged in a series of plexuses throughout the gut: the myenteric plexus and submucosal plexus. While it is known that enteric ganglia are derived from a stem cell population called the neural crest, mechanisms that dictate final neuropil plexus organization remain obscure. Recently, the vertebrate animal, zebrafish, has emerged as a useful model to understand ENS development, however knowledge of its developing myenteric plexus architecture was unknown. Here, we examine myenteric plexus of the maturing zebrafish larval fish histologically over time and find that it consists of a series of tight axon layers and long glial cell processes that wrap the circumference of the gut tube to completely encapsulate it, along all levels of the gut. By late larval stages, complexity of the myenteric plexus increases such that a layer of axons is juxtaposed to concentric layers of glial cells. Ultrastructurally, glial cells contain glial filaments and make intimate contacts with one another in long, thread-like projections. Conserved indicators of vesicular axon profiles are readily abundant throughout the larval plexus neuropil. Together, these data extend our understanding of myenteric plexus architecture in maturing zebrafish, thereby enabling functional studies of its formation in the future.

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