Response of Spatial Patterns of Denitrifying Bacteria Communities to Water Properties in the Stream Inlets at Dianchi Lake, China

Streams are an important sink for anthropogenic N owing to their hydrological connections with terrestrial systems, but main factors influencing the community structure and abundance of denitrifiers in stream water remain unclear. To elucidate the potential impact of varying water properties of different streams on denitrifiers, the abundance and community of three denitrifying genes coding for nitrite (nirK, nirS) and nitrous oxide (nosZ) reductase were investigated in 11 streams inlets at the north part of Dianchi Lake. The DGGE results showed the significant pairwise differences in community structure ofnirK, nirS, andnosZgenes among different streams. The results of redundancy analysis (RDA) confirmed that nitrogen and phosphorus concentrations, pH, and temperature in waters were the main environmental factors leading to a significant alteration in the community structure of denitrifiers among different streams. The denitrifying community size was assessed by quantitative PCR (qPCR) of thenirS, nirK, andnosZgenes. The abundance ofnirK, nirS, andnosZwas positively associated with concentrations of total N (TN) andPO43- (p<0.001). The difference in spatial patterns betweennirKandnirScommunity diversity, in combination with the spatial distribution of thenirS/nirKratio, indicated the occurrence of habitat selection for these two types of denitrifiers in the different streams. The results indicated that the varying of N species andPO43- together with pH and temperature would be the main factors shaping the community structure of denitrifiers. Meanwhile, the levels of N in water, together withPO43- , tend to affect the abundance of denitrifiers.

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Nitrogen and phosphorus relationships to benthic algal biomass in temperate streams

Canadian Journal of Fisheries and Aquatic Sciences ◽

10.1139/f02-063 ◽

2002 ◽

Vol 59 (5) ◽

pp. 865-874 ◽

Cited By ~ 268

Author(s):

Walter K Dodds ◽

Val H Smith ◽

Kirk Lohman

Keyword(s):

Stream Water ◽

Water Quality Monitoring ◽

The United States ◽

Anthropogenic Effects ◽

Nutrient Concentrations ◽

Monitoring Networks ◽

Stream Water Quality ◽

Nitrogen And Phosphorus ◽

Total N ◽

Temperate Streams

Knowledge of factors limiting benthic algal (periphyton) biomass is central to understanding energy flow in stream ecosystems and stream eutrophication. We used several data sets to determine how water column nutrients and nonnutrient factors are linked to periphytic biomass and if the ecoregion concept is applicable to nutrientperiphyton relationships. Literature values for seasonal means of biomass of periphyton, nutrient concentrations, and other stream characteristics were collected for almost 300 sampling periods from temperate streams. Data for benthic chlorophyll and nutrient concentrations from a subset of 620 stations in the United States National Stream Water-Quality Monitoring Networks were also analyzed. The greatest portion of variance in models for the mean and maximum biomass of benthic stream algae (about 40%) was explained by concentrations of total N and P. Breakpoint regression and a two-dimensional KolmogorovSmirnov statistical technique established significant breakpoints of about 30 µg total P·L1 and 40 µg total N·L1, above which mean chlorophyll values were substantially higher. Ecoregion effects on nutrientchlorophyll relationships were weak. Ecoregion effects were cross-correlated with anthropogenic effects such as percent urban and cropland area in the watershed and population density. Thus, caution is necessary to separate anthropogenic effects from natural variation at the ecoregion level.

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Evidence of Phosphate Mining and Agriculture Influence on Concentrations, Forms, and Ratios of Nitrogen and Phosphorus in a Florida River

Water ◽

10.3390/w13081064 ◽

2021 ◽

Vol 13 (8) ◽

pp. 1064

Author(s):

Shuiwang Duan ◽

Kamaljit Banger ◽

Gurpal S. Toor

Keyword(s):

Agricultural Land ◽

Water Discharge ◽

Mining Area ◽

Specific Conductance ◽

Organic N ◽

Wet Season ◽

Nitrogen And Phosphorus ◽

Total N ◽

Phosphate Mining ◽

Molar Ratios

Florida has a long history of phosphate-mining, but less is known about how mining affects nutrient exports to coastal waters. Here, we investigated the transport of inorganic and organic forms of nitrogen (N) and phosphorus (P) over 23 sampling events during a wet season (June–September) in primary tributaries and mainstem of Alafia River that drains into the Tampa Bay Estuary. Results showed that a tributary draining the largest phosphate-mining area (South Prong) had less flashy peaks, and nutrients were more evenly exported relative to an adjacent tributary (North Prong), highlighting the effectiveness of the mining reclamation on stream hydrology. Tributaries draining > 10% phosphate-mining area had significantly higher specific conductance (SC), pH, dissolved reactive P (DRP), and total P (TP) than tributaries without phosphate-mining. Further, mean SC, pH, and particulate reactive P were positively correlated with the percent phosphate-mining area. As phosphate-mining occurred in the upper part of the watershed, the SC, pH, DRP, and TP concentrations increased downstream along the mainstem. For example, the upper watershed contributed 91% of TP compared to 59% water discharge to the Alafia River. In contrast to P, the highest concentrations of total N (TN), especially nitrate + nitrite (NOx–N) occurred in agricultural tributaries, where the mean NOx–N was positively correlated with the percent agricultural land. Dissolved organic N was dominant in all streamwaters and showed minor variability across sites. As a result of N depletion and P enrichment, the phosphate-mining tributaries had significantly lower molar ratios of TN:TP and NOx–N:DRP than other tributaries. Bi-weekly monitoring data showed consistent increases in SC and DRP and a decrease in NOx–N at the South Prong tributary (highest phosphate-mining area) throughout the wet season, and different responses of dissolved inorganic nutrients (negative) and particulate nutrients (positive) to water discharge. We conclude that (1) watersheds with active and reclaimed phosphate-mining and agriculture lands are important sources of streamwater P and N, respectively, and (2) elevated P inputs from the phosphate-mining areas altered the N:P ratios in streamwaters of the Alafia River.

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Interaction between typical sulfonamides and bacterial diversity in drinking water

Journal of Water and Health ◽

10.2166/wh.2018.210 ◽

2018 ◽

Vol 16 (6) ◽

pp. 914-920 ◽

Cited By ~ 2

Author(s):

Qing Wu ◽

Shuqun Li ◽

Xiaofei Zhao ◽

Xinhua Zhao

Keyword(s):

Drinking Water ◽

Community Structure ◽

Microbial Community ◽

Bacterial Community ◽

Bacterial Diversity ◽

Microbial Community Structure ◽

Antibiotic Use ◽

Community Diversity ◽

Wide Range ◽

Growth Of Bacteria

Abstract The abuse of antibiotics is becoming more serious as antibiotic use has increased. The sulfa antibiotics, sulfamerazine (SM1) and sulfamethoxazole (SMZ), are frequently detected in a wide range of environments. The interaction between SM1/SMZ and bacterial diversity in drinking water was investigated in this study. The results showed that after treatment with SM1 or SMZ at four different concentrations, the microbial community structure of the drinking water changed statistically significantly compared to the blank sample. At the genus level, the proportions of the different bacteria in drinking water may affect the degradation of the SM1/SMZ. The growth of bacteria in drinking water can be inhibited after the addition of SM1/SMZ, and bacterial community diversity in drinking water declined in this study. Furthermore, the resistance gene sul2 was induced by SM1 in the drinking water.

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Diurnal and spatial (vertical) dynamics of nutrients (N, P, Si) in four sampling days (summer, fall, winter, and spring) in a tropical shallow reservoir and their relationships with the phytoplankton community

Brazilian Journal of Biology ◽

10.1590/s1519-69842005000100018 ◽

2005 ◽

Vol 65 (1) ◽

pp. 141-157 ◽

Cited By ~ 8

Author(s):

J. J. Ramírez ◽

C. E. M. Bicudo

Keyword(s):

Phytoplankton Community ◽

Southeastern Brazil ◽

Nitrogen And Phosphorus ◽

Vertical Variation ◽

Total N ◽

Phosphorus Forms ◽

Reactive Silica ◽

Photosynthetic Assimilation ◽

The City ◽

Total P

The vertical and diurnal variation of nitrogen and phosphorus forms, as well as that of soluble reactive silica (SRS), were studied in four sampling days at Garças reservoir, a shallow tropical one located in the city of São Paulo, in southeastern Brazil. Except for N-NH4, all other inorganic forms of nitrogen (N-NO2, N-NO3, and total N) demonstrated decreased concentrations toward the bottom of reservoir. Similarly, all showed significant diurnal differences on every sampling day, with increased values during the night due to absence of photosynthetic assimilation during that period. In the sampling days, these forms decreased on the spring sampling day due to the bloom of Microcystis registered during this period of the year. All three forms of phosphorus (SRP, particulate P, and total P) showed significant vertical variation, except on the fall sampling day. On the summer sampling day there was an increase of both total P and particulate P, the latter because it constitutes more than 70% of the total P during all sampling days. Hourly phosphorus variation was significant during all sampling days, except for the summer one. The SRS vertical variation was significant during all sampling days, except for that in the spring. It was also different hourly on sampling days.

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Effects of sediment nutrients and depth on small-scale spatial heterogeneity of submersed macrophyte communities in Lake Pleasant, Pennsylvania

Canadian Journal of Fisheries and Aquatic Sciences ◽

10.1139/f04-081 ◽

2004 ◽

Vol 61 (8) ◽

pp. 1493-1502 ◽

Cited By ~ 10

Author(s):

R K Johnson ◽

M L Ostrofsky

Keyword(s):

Organic Matter ◽

Community Structure ◽

Spatial Heterogeneity ◽

Environmental Gradients ◽

Small Scale ◽

Sediment Characteristics ◽

Available Nitrogen ◽

Total N ◽

Available N ◽

Prevailing Paradigm

Sediment concentrations of total and available nitrogen (N), phosphorus (P), and potassium (K) and organic matter from the littoral zone of Lake Pleasant, Pennsylvania, were highly variable. Only organic matter and total N were correlated with depth, however. This result suggests the existence of more complex environmental gradients than the prevailing paradigm of monotonic changes in sediment characteristics with increasing depth. The spatial heterogeneity of submersed aquatic plant communities was significantly correlated with depth, and available N and P. Canonical correspondence analysis demonstrated that these three factors explained 38% of the variance in community structure. Other sediment characteristics (available K, organic matter, and total N, P and K) were not significant by themselves, but all variables combined explained 63% of community-structure variance. Cluster analysis identified species or groups of species typical of endpoints on the depth versus nutrient axes. Myriophyllum exalbescens was typical of deep sites with relatively nutrient-rich sediments, whereas deep nutrient-poor sites were dominated by Vallisneria americana and Megalodonta beckii. Shallow nutrient-rich sites were dominated by several species of Potamogeton and Elodea canadensis, and shallow nutrient-poor sites were dominated by Heteranthera dubia and Najas flexilis. These results demonstrate the importance of sediment characteristics in determining macrophytes' community structure within lakes.

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Heterotrophic Bacterial Community Structure of Multistage Biofilters in a Commercial Pufferfish Takifugu rubripes RAS

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.726-731.1621 ◽

2013 ◽

Vol 726-731 ◽

pp. 1621-1627 ◽

Cited By ~ 2

Author(s):

Zhu Chen ◽

Ying Liu ◽

Liang Zi Liu ◽

Xiao Jing Wang ◽

Zhi Pei Liu ◽

...

Keyword(s):

Community Structure ◽

Microbial Community ◽

Bacterial Community ◽

Bacterial Community Structure ◽

Heterotrophic Bacteria ◽

Diversity Index ◽

Community Diversity ◽

Takifugu Rubripes ◽

Recirculating Aquaculture ◽

Microbial Resources

The success of a recirculating aquaculture system (RAS) greatly depends on the structure, dynamics and activities of microbial community. Heterotrophic bacteria as the major members play various roles. The heterotrophic bacterial community structure in threestaged biofilters was studied using four different media. 228 isolates belonging to 77species were obtained and affiliated toGammaproteobacteria,Alphaproteobacteria,Bacteroidetes,Firmicutes,ActinobacteriaandBetaproteobacteria.Gammaproteobacteriawas the predominant group. The concurrence was found between potential pathogens (VibrioandShewanella) and probiotics (BacillusandPseudomonas). On the basis of community diversity index, we could infer that differences existed between stages, and the diversity index increased along the biofilters. A comprehensive understanding of microbial community in RAS will be in favor of utilization of microbial resources and optimizing the culture systems' operation.

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Phytoplankton community structure in Menjer Lake, Central Java

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/869/1/012037 ◽

2021 ◽

Vol 869 (1) ◽

pp. 012037

Author(s):

F Sulawesty ◽

G P Yoga ◽

L Subehi ◽

R Rosidah

Keyword(s):

Community Structure ◽

Phytoplankton Community ◽

Diversity Index ◽

Nutrient Content ◽

Cell Counting ◽

Phytoplankton Community Structure ◽

Nitrogen And Phosphorus ◽

Central Java ◽

Phytoplankton Communities ◽

Ecological Pressure

Abstract The occurrence of land changes around Lake Menjer, Central Java Province will affect the condition of water quality subsequently will affect biota, including phytoplankton. The purposes of study was to analyze the composition and abundance of phytoplankton in Menjer Lake regarding to nutrient content i.e. nitrogen and phosphorus. Observations on the phytoplankton community were conducted in July and October 2019 at six locations in Menjer Lake. Water samples were taken at the water surface as much as 10 L filtered using plankton net. Identification was carried out under the inverted microscope Diaphot 300. The abundance was calculated using the Sedgwick Rafter cell counting (SRCC) method. Analysis of the phytoplankton community structure was derived by calculating the Diversity Index, Evenness Index, and Simpson Dominance Index. The nutrient content in Menjer Lake influences the structure of phytoplankton communities quantitatively and qualitatively. Observation showed that the diversity of phytoplankton was low and there was one species dominanted, the result explained that the phytoplankton community in Menjer Lake is unstable and there is ecological pressure on the community. It can be concluded that the improvement of environmental conditions around Menjer Lake is the basis for the sustainable management of Menjer Lake.

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Distribution of Triarrhena lutarioriparia and its reserve characteristics of nitrogen and phosphorus in Dongting Lake

E3S Web of Conferences ◽

10.1051/e3sconf/202123701004 ◽

2021 ◽

Vol 237 ◽

pp. 01004

Author(s):

Youze Xu ◽

Guangyi Fu ◽

Nan Tang ◽

Zhonghao He ◽

Lincheng Jian ◽

...

Keyword(s):

Scientific Basis ◽

Dongting Lake ◽

Lake Basin ◽

Nitrogen And Phosphorus ◽

Total N ◽

Remote Sensing Interpretation ◽

Storage Characteristics ◽

Total P ◽

Lake Wetlands

Triarrhena lutarioriparia, a typical and most abundant macrophyte in Dongting lake wetland, was in the state of abandonment following the papermaking industry revocation in the lake basin. In order to provide scientific basis for precise management of T. lutarioriparia, the T. lutarioriparia distribution charateristics in Dongting Lake and its storage characteristics of nutrients were investigated in this study. Remote sensing interpretation results showed that the total area of T. lutarioriparia in Dongting Lake wetland was 58, 450 ha, 48.31% of which distributed in South Doting Lake wetlands. The nutrients contents were significantly different in T. lutarioriparia tissues, ranking in the descending order of spikes (TN 27.90 mg/g, TP 3.46 mg/g)>leaves (TN 16.38 mg/g, TP 2.11 mg/g)>stems (TN 5.38 mg/g, TP 0.85 mg/g). The total P quantities in each T. lutarioriparia tissue were ranked in the order: stems (560.26 t)>leaves (396.52 t)>spikes (284.67 t), while the total N quantities were within the range of 2170.02-2801.3 t. It was estimated that about 7712.99 t of TN and 1241.45 t of TP were annually removed from Dongting Lake by reaping T. lutarioriparia. The nutrients stored in the dead tissues of T. lutarioriparia might possess non-negligible impact on the water quality of Doting Lake.

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Land use influences stream bacterial communities in lowland tropical watersheds

Scientific Reports ◽

10.1038/s41598-021-01193-7 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Karina A. Chavarria ◽

Kristin Saltonstall ◽

Jorge Vinda ◽

Jorge Batista ◽

Megan Lindmark ◽

...

Keyword(s):

Water Quality ◽

Land Use ◽

Bacterial Communities ◽

Secondary Forest ◽

Stream Water ◽

Community Diversity ◽

Forested Catchments ◽

Unifrac Distance ◽

Cattle Pasture ◽

The Impact

AbstractLand use is known to affect water quality yet the impact it has on aquatic microbial communities in tropical systems is poorly understood. We used 16S metabarcoding to assess the impact of land use on bacterial communities in the water column of four streams in central Panama. Each stream was influenced by a common Neotropical land use: mature forest, secondary forest, silvopasture and traditional cattle pasture. Bacterial community diversity and composition were significantly influenced by nearby land uses. Streams bordered by forests had higher phylogenetic diversity (Faith’s PD) and similar community structure (based on weighted UniFrac distance), whereas the stream surrounded by traditional cattle pasture had lower diversity and unique bacterial communities. The silvopasture stream showed strong seasonal shifts, with communities similar to forested catchments during the wet seasons and cattle pasture during dry seasons. We demonstrate that natural forest regrowth and targeted management, such as maintaining and restoring riparian corridors, benefit stream-water microbiomes in tropical landscapes and can provide a rapid and efficient approach to balancing agricultural activities and water quality protection.

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