Pasteurized Orange Juice Rich in Carotenoids Protects Caenorhabditis elegans against Oxidative Stress and β-Amyloid Toxicity through Direct and Indirect Mechanisms

‘Cara Cara’ is a red orange (Citrus sinensis (L.) Osbeck) variety originally from Venezuela characterized by a significantly higher and diversified carotenoid content including higher-concentration lycopene, all-E-β-carotene, phytoene, and other carotenoids when compared with the carotenoid profile of its isogenic blond counterpart ‘Bahia’, also known as Washington navel. The exceptionally high carotenoid content of ‘Cara Cara’ is of special interest due to its neuroprotective potential. Here, we used the nematode Caenorhabditis elegans to analyze the antioxidant effect and the protection against β-amyloid-induced toxicity of pasteurized orange juice (POJ) obtained from ‘Cara Cara’ and compare to that from ‘Bahia’. POJ treatment reduced the endogenous ROS levels and increased the worm’s survival rate under normal and oxidative stress conditions. POJ treatment also upregulated the expression of antioxidant (gcs-1, gst-4, and sod-3) and chaperonin (hsp-16.2) genes. Remarkably, ROS reduction, gene expression activation, oxidative stress resistance, and longevity extension were significantly increased in the animals treated with ‘Cara Cara’ orange juice compared to animals treated with ‘Bahia’ orange juice. Furthermore, the body paralysis induced by β-amyloid peptide was delayed by both POJs but the mean paralysis time for the worms treated with ‘Cara Cara’ orange juice was significantly higher compared to ‘Bahia’ orange juice. Our mechanistic studies indicated that POJ-reduced ROS levels are primarily a result of the direct scavenging action of natural compounds available in the orange juice. Moreover, POJ-induced gst-4::GFP expression and –increased stress resistance was dependent of the SKN-1/Nrf2 transcription factor. Finally, the transcription factors SKN-1, DAF-16, and HSF-1 were required for the POJ-mediated protective effect against Aβ toxicity. Collectively, these results suggest that orange juice from ‘Cara Cara’ induced a stronger response against oxidative stress and β-amyloid toxicity compared to orange juice from ‘Bahia’ possibly due to its higher carotenoid content.

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Streblus asper Lour. exerts MAPK and SKN-1 mediated anti-aging, anti-photoaging activities and imparts neuroprotection by ameliorating Aβ in Caenorhabditis elegans

Nutrition and Healthy Aging ◽

10.3233/nha-210121 ◽

2021 ◽

pp. 1-17

Author(s):

Mani Iyer Prasanth ◽

James Michael Brimson ◽

Dicson Sheeja Malar ◽

Anchalee Prasansuklab ◽

Tewin Tencomnao

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Stress Resistance ◽

Vital Role ◽

Transgenic Strain ◽

Wild Type ◽

Neuroprotective Effects ◽

C Elegans ◽

Streblus Asper

BACKGROUND: Streblus asper Lour., has been reported to have anti-aging and neuroprotective efficacies in vitro. OBJECTIVE: To analyze the anti-aging, anti-photoaging and neuroprotective efficacies of S. asper in Caenorhabditis elegans. METHODS: C. elegans (wild type and gene specific mutants) were treated with S. asper extract and analyzed for lifespan and other health benefits through physiological assays, fluorescence microscopy, qPCR and Western blot. RESULTS: The plant extract was found to increase the lifespan, reduce the accumulation of lipofuscin and modulate the expression of candidate genes. It could extend the lifespan of both daf-16 and daf-2 mutants whereas the pmk-1 mutant showed no effect. The activation of skn-1 was observed in skn-1::GFP transgenic strain and in qPCR expression. Further, the extract can extend the lifespan of UV-A exposed nematodes along with reducing ROS levels. Additionally, the extract also extends lifespan and reduces paralysis in Aβ transgenic strain, apart from reducing Aβ expression. CONCLUSIONS: S. asper was able to extend the lifespan and healthspan of C. elegans which was independent of DAF-16 pathway but dependent on SKN-1 and MAPK which could play a vital role in eliciting the anti-aging, anti-photoaging and neuroprotective effects, as the extract could impart oxidative stress resistance and neuroprotection.

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A Cocoa Peptide Protects Caenorhabditis elegans from Oxidative Stress and β-Amyloid Peptide Toxicity

PLoS ONE ◽

10.1371/journal.pone.0063283 ◽

2013 ◽

Vol 8 (5) ◽

pp. e63283 ◽

Cited By ~ 39

Author(s):

Patricia Martorell ◽

Esther Bataller ◽

Silvia Llopis ◽

Núria Gonzalez ◽

Beatriz Álvarez ◽

...

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Amyloid Peptide ◽

Β Amyloid ◽

Β Amyloid Peptide

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Chlorophyll enhances oxidative stress tolerance inCaenorhabditis elegansand extends its lifespan

PeerJ ◽

10.7717/peerj.1879 ◽

2016 ◽

Vol 4 ◽

pp. e1879 ◽

Cited By ~ 22

Author(s):

Erjia Wang ◽

Michael Wink

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Secondary Metabolites ◽

Model System ◽

The Body ◽

Aging Effects ◽

Oxidative Stress Tolerance ◽

C Elegans ◽

Dependent Pathway

Green vegetables are thought to be responsible for several beneficial properties such as antioxidant, anti-mutagenic, and detoxification activities. It is not known whether these effects are due to chlorophyll which exists in large amounts in many foods or result from other secondary metabolites. In this study, we used the model systemCaenorhabditis elegansto investigate the anti-oxidative and anti-aging effects of chlorophyllin vivo. We found that chlorophyll significantly improves resistance to oxidative stress. It also enhances the lifespan ofC. elegansby up to 25% via activation of the DAF-16/FOXO-dependent pathway. The results indicate that chlorophyll is absorbed by the worms and is thus bioavailable, constituting an important prerequisite for antioxidant and longevity-promoting activities inside the body. Our study thereby supports the view that green vegetables may also be beneficial for humans.

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Ingredients in Zijuan Pu’er Tea Extract Alleviate β-Amyloid Peptide Toxicity in a Caenorhabditis elegans Model of Alzheimer’s Disease Likely through DAF-16

Molecules ◽

10.3390/molecules24040729 ◽

2019 ◽

Vol 24 (4) ◽

pp. 729 ◽

Cited By ~ 2

Author(s):

Fangzhou Du ◽

Lin Zhou ◽

Yan Jiao ◽

Shuju Bai ◽

Lu Wang ◽

...

Keyword(s):

Oxidative Stress ◽

Alzheimer’S Disease ◽

Alzheimer's Disease ◽

Caenorhabditis Elegans ◽

Protective Effect ◽

Nuclear Translocation ◽

Amyloid Β ◽

Amyloid Peptide ◽

C Elegans ◽

Β Amyloid

Amyloid-β, one of the hallmarks of Alzheimer’s disease (AD), is toxic to neurons and can also cause brain cell death. Oxidative stress is known to play an important role in AD, and there is strong evidence that oxidative stress is associated with amyloid-β. In the present study we report the protective effect of Zijuan Pu’er tea water extract (ZTWE) and the mixture of main ingredients (+)-catechins, caffeine and procyanidin (MCCP) in ZTWE on β-amyloid-induced toxicity in transgenic Caenorhabditis elegans (C. elegans) CL4176 expressing the human Aβ1–42 gene. ZTWE, (+)-catechins, caffeine, procyanidin and MCCP delayed the β-amyloid-induced paralysis to different degrees. The MCCP treatment did not affect the transcript abundance of amyloid-β transgene (amy-1); however, Thioflavin T staining showed a significant decrease in Aβ accumulation compared to untreated worms. Further research using transgenic worms found that MCCP promoted the translocation of DAF-16 from cytoplasm to nucleus and increased the expression of superoxide dismutase 3 (SOD-3). In addition, MCCP decreased the reactive oxygen species (ROS) content and increased the SOD activity in CL4176 worms. In conclusion, the results suggested that MCCP had a significant protective effect on β-amyloid-induced toxicity in C. elegans by reducing β-amyloid aggregation and inducing DAF-16 nuclear translocation that could activate the downstream signal pathway and enhance resistance to oxidative stress.

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Production of YP170 Vitellogenins Promotes Intestinal Senescence in Caenorhabditis elegans

The Journals of Gerontology Series A ◽

10.1093/gerona/glz067 ◽

2019 ◽

Vol 74 (8) ◽

pp. 1180-1188 ◽

Cited By ~ 2

Author(s):

Thanet Sornda ◽

Marina Ezcurra ◽

Carina Kern ◽

Evgeniy R Galimov ◽

Catherine Au ◽

...

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Life Span ◽

Stress Resistance ◽

Later Life ◽

Abundant Species ◽

Yolk Proteins ◽

Oxidative Stress Resistance ◽

Vitellogenin Synthesis ◽

Intestinal Atrophy

Abstract During aging, etiologies of senescence cause multiple pathologies, leading to morbidity and death. To understand aging requires identification of these etiologies. For example, Caenorhabditis elegans hermaphrodites consume their own intestinal biomass to support yolk production, which in later life drives intestinal atrophy and ectopic yolk deposition. Yolk proteins (YPs; vitellogenins) exist as three abundant species: YP170, derived from vit-1–vit-5; and YP115 and YP88, derived from vit-6. Here, we show that inhibiting YP170 synthesis leads to a reciprocal increase in YP115/YP88 levels and vice versa, an effect involving posttranscriptional mechanisms. Inhibiting YP170 production alone, despite increasing YP115/YP88 synthesis, reduces intestinal atrophy as much as inhibition of all YP synthesis, which increases life span. By contrast, inhibiting YP115/YP88 production alone accelerates intestinal atrophy and reduces life span, an effect that is dependent on increased YP170 production. Thus, despite copious abundance of both YP170 and YP115/YP88, only YP170 production is coupled to intestinal atrophy and shortened life span. In addition, increasing levels of YP115/YP88 but not of YP170 increases resistance to oxidative stress; thus, longevity resulting from reduced vitellogenin synthesis is not attributable to oxidative stress resistance.

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Faculty Opinions recommendation of Influence of resveratrol on oxidative stress resistance and life span in Caenorhabditis elegans.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.726214939.793515605 ◽

2016 ◽

Author(s):

David Lombard

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Life Span ◽

Stress Resistance ◽

Oxidative Stress Resistance

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Lentinan extends lifespan and increases oxidative stress resistance through DAF-16 and SKN-1 pathways in Caenorhabditis elegans

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2022.01.071 ◽

2022 ◽

Author(s):

Huimin Liu ◽

Ying Wang ◽

Weiping Zhang ◽

Weichao Sun ◽

Xiaoxue Ji ◽

...

Keyword(s):

Oxidative Stress ◽

Caenorhabditis Elegans ◽

Stress Resistance ◽

Oxidative Stress Resistance

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Carqueja (Baccharis trimera) Protects against Oxidative Stress andβ-Amyloid-Induced Toxicity inCaenorhabditis elegans

Oxidative Medicine and Cellular Longevity ◽

10.1155/2015/740162 ◽

2015 ◽

Vol 2015 ◽

pp. 1-15 ◽

Cited By ~ 9

Author(s):

Franciny Aparecida Paiva ◽

Larissa de Freitas Bonomo ◽

Patrícia Ferreira Boasquivis ◽

Igor Thadeu Borges Raposo de Paula ◽

Joyce Ferreira da Costa Guerra ◽

...

Keyword(s):

Oxidative Stress ◽

Stress Resistance ◽

Native Plant ◽

Hydroalcoholic Extract ◽

C Elegans ◽

Amyloid Toxicity ◽

First Time ◽

Baccharis Trimera

Carqueja (Baccharis trimera) is a native plant found throughout South America. Several studies have shown that Carqueja has antioxidant activityin vitro, as well as anti-inflammatory, antidiabetic, analgesic, antihepatotoxic, and antimutagenic properties. However, studies regarding its antioxidant potentialin vivoare limited. In this study, we usedCaenorhabditis elegansas a model to examine the antioxidant effects of a Carqueja hydroalcoholic extract (CHE) on stress resistance and lifespan and to investigate whether CHE has a protective effect in aC. elegansmodel for Alzheimer's disease. Here, we show for the first time, usingin vivoassays, that CHE treatment improved oxidative stress resistance by increasing survival rate and by reducing ROS levels under oxidative stress conditions independently of the stress-related signaling pathways (p38, JNK, and ERK) and transcription factors (SKN-1/Nrf and DAF-16/Foxo) tested here. CHE treatment also increased the defenses againstβ-amyloid toxicity inC. elegans, in part by increasing proteasome activity and the expression of two heat shock protein genes. Our findings suggest a potential neuroprotective use for Carqueja, supporting the idea that dietary antioxidants are a promising approach to boost the defensive systems against stress and neurodegeneration.

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