Encapsulated Mulberry Fruit Extract Alleviates Changes in an Animal Model of Menopause with Metabolic Syndrome

Currently, the therapeutic strategy against metabolic syndrome and its complications is required due to the increasing prevalence and its impact. Due to the benefits of both mulberry fruit extract and encapsulation technology, we hypothesized that encapsulated mulberry fruit extract (MME) could improve metabolic parameters and its complication risk in postmenopausal metabolic syndrome. To test this hypothesis, female Wistar rats were induced experimental menopause with metabolic syndrome by bilateral ovariectomy (OVX) and high-carbohydrate high-fat (HCHF) diet. Then, they were orally given MME at doses of 10, 50, and 250 mg/kg BW for 8 weeks and the parameters, such as percentage of body weight gain, total cholesterol, triglycerides, HDL-C, LDL-C, atherogenic index, fasting blood glucose, plasma glucose area under the curve, serum angiotensin-converting enzyme (ACE), oxidative stress status, histology, and protein expression of PPAR-γ, TNF-α, and NF-κB in adipose tissues were determined. MME improved body weight gain, adiposity index, glucose intolerance, lipid profiles, atherogenic index, ACE, oxidative stress status, and protein expression of TNF-αand NF-κB. Moreover, MME attenuated adipocyte hypertrophy and enhanced PPAR-γexpression. Taken altogether, MME decreased metabolic syndrome and its complication via the increased PPAR-γexpression. Therefore, MME is the potential candidate for improving metabolic syndrome and its related complications. However, further research in clinical trial is still necessary.

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Insecticide chlorpyrifos and fungicide carbendazim, common food contaminants mixture, induce hepatic, renal, and splenic oxidative damage in female rats

Human & Experimental Toxicology ◽

10.1177/0960327116652459 ◽

2016 ◽

Vol 36 (5) ◽

pp. 483-493 ◽

Cited By ~ 16

Author(s):

AO Abolaji ◽

IO Awogbindin ◽

IA Adedara ◽

EO Farombi

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Weight Gain ◽

Oxidative Damage ◽

Body Weight Gain ◽

The Body ◽

Female Rats ◽

Exposure Group ◽

Food Contaminants ◽

Antioxidant Enzymes Activities

The fungicide carbendazim (CBZ) and insecticide chlorpyrifos (CPF) are currently applied together by farmers for the control of pests. Here, we investigated the impacts of 7 days oral co-exposure to 10 mg/kg body weight of CPF and 50 mg/kg body weight of CBZ on selected oxidative stress and antioxidant biomarkers in the liver, kidney, and spleen of female rats. The results showed that while the body weight gain and relative organ weights were not significantly affected after separate exposure to CPF and CBZ, there was a significant decrease in the body weight gain with concomitant increases in the relative kidney and spleen weights of rats treated with the mixture. Also, CPF and CBZ co-exposure significantly increased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine ( p < 0.05) when compared with the groups treated with CBZ or CPF alone and the control. The significant decreases in both antioxidant enzymes activities and nonenzymatic antioxidant level following individual administration of CPF and CBZ to rats were intensified in the co-exposure group ( p < 0.05). Additionally, the marked increases in the levels of oxidative stress indices in liver, kidney, and spleen of rats treated with CPF or CBZ alone were intensified in the co-exposure group ( p < 0.05). Histopathologically, co-exposure to CPF and CBZ exacerbates their individual effects on the liver, kidney, and spleen. These findings showed that co-exposure to CPF and CBZ in rats elicited more severe oxidative damage on the liver, kidney, and spleen of the rats, indicative of an additive effect compared to CPF or CBZ alone and as such, may pose a greater environmental risk to humans.

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Antioxidant Activity of Sprouts Extracts Is Correlated with Their Anti-Obesity and Anti-Inflammatory Effects in High-Fat Diet-Fed Mice

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/8367802 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Chung Shil Kwak ◽

Mi-Ju Kim ◽

Sunyeong Park ◽

In Gyu Kim

Keyword(s):

Oxidative Stress ◽

Antioxidant Activity ◽

Body Weight ◽

Adipose Tissue ◽

Weight Gain ◽

Body Weight Gain ◽

Mrna Levels ◽

Adipocyte Size ◽

Anti Inflammatory ◽

Visceral Adipose

Obesity is closely associated with oxidative stress and chronic inflammation leading to related metabolic diseases. Some natural extracts or polyphenols reportedly possess anti-obesity and anti-inflammatory effects as well as antioxidant activity. In this study, we assessed the correlations between the antioxidant, anti-obesity, and anti-inflammatory activities of plant extracts with potent antioxidant activity in diet-induced obese mice. Sprouts of Cedrela sinensis (CS) and Oenothera biennis L. (OB) were selected as the most potent antioxidant plant based on analysis of in vitro antioxidant activity of the extracts of ten different edible plants. C57BL/6 mice were fed with a high-fat diet (HFD) and orally treated with 50% ethanol extract of CS or OB at 50 or 100 mg/kg body weight 5 days a week for 14 weeks. Body weight gain, weight of adipose tissue, adipocyte size, and levels of lipid metabolism, inflammation, and oxidative stress markers were investigated. The CS or OB extract reduced body weight gain, visceral adipose tissue weight, adipocyte size, and plasma leptin levels, and expressions of adipogenic genes (PPARγ and fatty acid synthase) in the adipose tissue and liver of HFD-fed mice. Both extracts also reduced mRNA levels of pro-inflammatory cytokines (IL-6 and TNF-α) and oxidative stress-related genes (heme oxygenase- (HO-) 1 and p40phox). Body weight gain of mice was significantly correlated with visceral adipose tissue weight and adipocyte size. Body weight gain and adipocyte size were significantly correlated with plasma total cholesterol and 8-epi PGF2α levels, mRNA levels of leptin, HO-1, p40phox, and CD-11 in the adipose tissue, and mRNA levels of TNF-α in the adipose tissue and liver. These results suggest that the CS and OB extracts with potent antioxidant activity may inhibit fat deposition in adipose tissue and subsequent inflammation.

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The Vagus Nerve and Spleen: Influence on White Adipose Mass and Histology of Obese and Non-obese Rats

Frontiers in Physiology ◽

10.3389/fphys.2021.672027 ◽

2021 ◽

Vol 12 ◽

Author(s):

Joice Cristina Kuchler ◽

Bruna Schumaker Siqueira ◽

Vanessa Marieli Ceglarek ◽

Fernanda Vigilato Chasko ◽

Isllany Carvalho Moura ◽

...

Keyword(s):

Body Weight ◽

Weight Gain ◽

Protein Expression ◽

Vagus Nerve ◽

Plasma Levels ◽

Body Weight Gain ◽

Monosodium Glutamate ◽

Health State ◽

Dependent Manner ◽

Obese Rats

The vagus nerve (VN) and spleen represent a complex interface between neural and immunological functions, affecting both energy metabolism and white adipose tissue (WAT) content. Here, we evaluated whether vagal and splenic axis participates in WAT mass regulation in obese and non-obese male Wistar rats. High doses of monosodium glutamate (M; 4 g/Kg) were administered during the neonatal period to induce hypothalamic lesion and obesity (M-Obese rats). Non-obese or Control (CTL) rats received equimolar saline. At 60 days of life, M-Obese and CTL rats were randomly distributed into experimental subgroups according to the following surgical procedures: sham, subdiaphragmatic vagotomy (SV), splenectomy (SPL), and SV + SPL (n = 11 rats/group). At 150 days of life and after 12 h of fasting, rats were euthanized, blood was collected, and the plasma levels of glucose, triglycerides, cholesterol, insulin, and interleukin 10 (IL10) were analyzed. The visceral and subcutaneous WAT depots were excised, weighed, and histologically evaluated for number and size of adipocytes as well as IL10 protein expression. M-Obese rats showed higher adiposity, hyperinsulinemia, hypertriglyceridemia, and insulin resistance when compared with CTL groups (p < 0.05). In CTL and M-Obese rats, SV reduced body weight gain and triglycerides levels, diminishing adipocyte size without changes in IL10 expression in WAT (p< 0.05). The SV procedure resulted in high IL10 plasma levels in CTL rats, but not in the M-Obese group. The splenectomy prevented the SV anti-adiposity effects, as well as blocked the elevation of IL10 levels in plasma of CTL rats. In contrast, neither SV nor SPL surgeries modified the plasma levels of IL10 and IL10 protein expression in WAT from M-Obese rats. In conclusion, vagotomy promotes body weight and adiposity reduction, elevating IL10 plasma levels in non-obese animals, in a spleen-dependent manner. Under hypothalamic obesity conditions, VN ablation also reduces body weight gain and adiposity, improving insulin sensitivity without changes in IL10 protein expression in WAT or IL10 plasma levels, in a spleen-independent manner. Our findings indicate that the vagal-spleen axis influence the WAT mass in a health state, while this mechanism seems to be disturbed in hypothalamic obese animals.

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Perbandingan Aktivitas Ekstrak Kulit Buah Manggis dan Berbagai Antioksidan terhadap Penampilan Broiler

Jurnal Penelitian Pertanian Terapan ◽

10.25181/jppt.v15i1.114 ◽

2017 ◽

Vol 15 (1) ◽

Author(s):

Agung Adi Candra

Keyword(s):

Body Weight ◽

Weight Gain ◽

Vitamin E ◽

Broiler Chickens ◽

Body Weight Gain ◽

Feed Conversion ◽

Fruit Extract ◽

Mangosteen Peel ◽

Carcass Performance ◽

Peel Extract

The aims of this study to determine the mangosteen peel extract on performance, percentage and carcass performance of broilers. This study used 200 broiler chickens were distributed into five treatments with 10 replications and each group of 4 broiler. The treatment groups were: control / blank, broilers with the addition of anti-oxidants synthesis (vitastress), the chickens are kept by using tocopherol, extracts of mangosteen rind 60 mg, and mangosteen peel extract 120 mg. Broilers were reared in the stable open as tropical enclosure models show weight gain diverse. The highest increase in keoompok mangosteen rind flour administration of 120 mg (equivalent to 2 capsules administration) followed by administration of vitamin E, an antioxidant synthesis and control. Mangosteen fruit extract 120 mg, equivalent to 2 capsules administration can increase body weight gain. While the mangosteen fruit extract 60 (1 capsule) gives the equivalent effect of vitamin E and antioksidang synthesis on the market. This implies the work of the mangosteen fruit extract capable meningkan body weight gain in giving 120 mg (2 capsules per cow per day). While the feed conversion rate (feed Conversion ratio) or defined as the ratio of the ability to convert feed into the ration in the treatment group in order from the smallest is the group with the administration of 2 capsules of 1.04 mangosteen mangosteen deilanjutkan 1 capsule of 1.3; antioxidants and vitamin E sinetesis of 1.4 and 1.7 control. Mangosteen 2 capsules a day is able to provide the ability to convert 1.04 kg of feed into 1 kg of meat. It gives an overview of the advantages for the farmer to use mangosteen peel extract as an antioxidant. Keywords: Mangosteen, Performa, antioxidant, broiler

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CHARD (BETA VULGARIS VAR. CICLA) EXTRACT MODULATES ZINC STATUS, GLUCOSE LEVEL AND ANTIOXYDANT VALUES IN DIABETIC RATS FED ZINC DEFICIENCY DIET

International Journal of Pharmacy and Pharmaceutical Sciences ◽

10.22159/ijpps.2017v9i10.19315 ◽

2017 ◽

Vol 9 (10) ◽

pp. 297 ◽

Cited By ~ 1

Author(s):

Malika Hamdiken ◽

Zine Kechrid

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Weight Gain ◽

Beta Vulgaris ◽

Zinc Deficiency ◽

Body Weight Gain ◽

Diabetic Rats ◽

The Other ◽

Zinc Status ◽

Deficient Diet

Objective: Oxidative stress which comes from hyperglycemia, it accelerates the development of cellular and vascular damage complications in diabetes, but the antioxidants may play a beneficial role in its prevention. Several plants extracts have an antioxidant activity and the ability to reduce oxidative stress in diabetes. Thus this study was conducted to investigate the effect of Beta vulgaris var cicla extract on zinc status, glucose concentration and antioxidant parameters in streptozotocin-diabetic rats fed zinc deficiency diet.Methods: Twenty-eight male albino (Wistar) rats were divided into four groups: two groups fed a zinc-sufficient diet one non-diabetic and the other diabetic, while the others two diabetic groups were fed a zinc-deficient diet, one non-treated group and the other treated with the extract of Beta vulgaris var cicla. After 21 d of dietary manipulation, fasting animals were scarified. Blood glucose, tissues zinc (femur, liver, kidney), malondialdehyde (MDA), reduced glutathione (GSH), glutathione peroxidase (GSH-Px) and glutathione-S-transferase (GST) were evaluated.Results: Body weight gain of zinc-deficient diabetic animals was lower than that of zinc-adequate diabetic animals. It was noticed also that inadequate dietary zinc intake increased glucose and MDA levels. In addition, zinc deficiency diet led to a decrease in zinc tissues, GSH concentration both GST and GSH-Px activities. However, Oral administration of Beta vulgaris extract significantly decreased both serum glucose and MDA (p<0.001) levels, with a significant increase in body weight gain (p<0.001), GSH concentration (p<0.05, P<0.001), GST (p<0.05, p<0.001) and GSH-Px (p<0.001) activities.Conclusion: The present study showed that Beta vulgaris var cicla supplementation presumably acting as an antioxidant, and it can be a natural source for the reduction of diabetes development caused by zinc deficiency.

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Cyclopiazonic acid augments the hepatic and renal oxidative stress in broiler chicks

Human & Experimental Toxicology ◽

10.1177/0960327110384285 ◽

2010 ◽

Vol 30 (8) ◽

pp. 910-919 ◽

Cited By ~ 9

Author(s):

H. Malekinejad ◽

P. Akbari ◽

M. Allymehr ◽

R. Hobbenaghi ◽

A. Rezaie

Keyword(s):

Oxidative Stress ◽

Body Weight ◽

Uric Acid ◽

Weight Gain ◽

Body Weight Gain ◽

Cyclopiazonic Acid ◽

Cell Swelling ◽

Broiler Chicks ◽

Mda Content ◽

Glutamyl Transferase

Generation of reactive oxygen species (ROS) leads to serious tissue injuries. The effect of cyclopiazonic acid (CPA) on oxidative stress markers in the liver and kidneys of broiler chicks was studied. Ten-day-old male broiler chicks (Ross 308) were assigned into the control and test groups, which received normal saline and 10, 25, and 50 μg/kg CPA, respectively, for 28 days. Body weight gain, serum level of alkaline phosphatase (ALP), γ-glutamyl transferase (GGT), uric acid, creatinine, and blood urea nitrogen (BUN) were measured after 2 and 4 weeks exposure. Moreover, the total thiol molecules (TTM) and malondialdehyde (MDA) content of the liver and kidneys were assessed. No significant differences (p > 0.05) were found in body weight gain between the control and test groups. Whereas, the hepatic weight increased significantly (p < 0.05) in animals that received 25 and 50 μg/kg CPA. Both ALP and GGT level in serum were elevated in comparison to the control group. CPA also resulted in uric acid, creatinine, and BUN enhancement in broilers. The MDA content of the liver and kidneys showed remarkable increase. By contrast, the TTM levels in the liver and kidneys were significantly (p < 0.05) attenuated. Histopathological findings confirmed the biochemical changes in either organ characterized by inflammatory cells infiltration along with severe congestion and cell swelling, suggesting an inflammatory response. These data suggest that exposure to CPA resulted in hepatic and renal disorders, which were reflected as biochemical markers alteration and pathological injuries in either organ. The biochemical alteration and pathological abnormalities may be attributed to CPA-induced oxidative stress.

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