Cyclopiazonic acid augments the hepatic and renal oxidative stress in broiler chicks

2010 ◽  
Vol 30 (8) ◽  
pp. 910-919 ◽  
Author(s):  
H. Malekinejad ◽  
P. Akbari ◽  
M. Allymehr ◽  
R. Hobbenaghi ◽  
A. Rezaie
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Uric Acid ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Cyclopiazonic Acid ◽  
Cell Swelling ◽  
Broiler Chicks ◽  
Mda Content ◽  
Glutamyl Transferase

Generation of reactive oxygen species (ROS) leads to serious tissue injuries. The effect of cyclopiazonic acid (CPA) on oxidative stress markers in the liver and kidneys of broiler chicks was studied. Ten-day-old male broiler chicks (Ross 308) were assigned into the control and test groups, which received normal saline and 10, 25, and 50 μg/kg CPA, respectively, for 28 days. Body weight gain, serum level of alkaline phosphatase (ALP), γ-glutamyl transferase (GGT), uric acid, creatinine, and blood urea nitrogen (BUN) were measured after 2 and 4 weeks exposure. Moreover, the total thiol molecules (TTM) and malondialdehyde (MDA) content of the liver and kidneys were assessed. No significant differences (p > 0.05) were found in body weight gain between the control and test groups. Whereas, the hepatic weight increased significantly (p < 0.05) in animals that received 25 and 50 μg/kg CPA. Both ALP and GGT level in serum were elevated in comparison to the control group. CPA also resulted in uric acid, creatinine, and BUN enhancement in broilers. The MDA content of the liver and kidneys showed remarkable increase. By contrast, the TTM levels in the liver and kidneys were significantly (p < 0.05) attenuated. Histopathological findings confirmed the biochemical changes in either organ characterized by inflammatory cells infiltration along with severe congestion and cell swelling, suggesting an inflammatory response. These data suggest that exposure to CPA resulted in hepatic and renal disorders, which were reflected as biochemical markers alteration and pathological injuries in either organ. The biochemical alteration and pathological abnormalities may be attributed to CPA-induced oxidative stress.

scholarly journals Encapsulated Mulberry Fruit Extract Alleviates Changes in an Animal Model of Menopause with Metabolic Syndrome

2019 ◽  
Vol 2019 ◽  
pp. 1-23 ◽  
Author(s):  
Jintanaporn Wattanathorn ◽  
Supannika Kawvised ◽  
Wipawee Thukham-mee
Keyword(s):  
Oxidative Stress ◽  
Metabolic Syndrome ◽  
Body Weight ◽  
Weight Gain ◽  
Protein Expression ◽  
Body Weight Gain ◽  
Atherogenic Index ◽  
Fruit Extract ◽  
Mulberry Fruit ◽  
Oxidative Stress Status

Currently, the therapeutic strategy against metabolic syndrome and its complications is required due to the increasing prevalence and its impact. Due to the benefits of both mulberry fruit extract and encapsulation technology, we hypothesized that encapsulated mulberry fruit extract (MME) could improve metabolic parameters and its complication risk in postmenopausal metabolic syndrome. To test this hypothesis, female Wistar rats were induced experimental menopause with metabolic syndrome by bilateral ovariectomy (OVX) and high-carbohydrate high-fat (HCHF) diet. Then, they were orally given MME at doses of 10, 50, and 250 mg/kg BW for 8 weeks and the parameters, such as percentage of body weight gain, total cholesterol, triglycerides, HDL-C, LDL-C, atherogenic index, fasting blood glucose, plasma glucose area under the curve, serum angiotensin-converting enzyme (ACE), oxidative stress status, histology, and protein expression of PPAR-γ, TNF-α, and NF-κB in adipose tissues were determined. MME improved body weight gain, adiposity index, glucose intolerance, lipid profiles, atherogenic index, ACE, oxidative stress status, and protein expression of TNF-αand NF-κB. Moreover, MME attenuated adipocyte hypertrophy and enhanced PPAR-γexpression. Taken altogether, MME decreased metabolic syndrome and its complication via the increased PPAR-γexpression. Therefore, MME is the potential candidate for improving metabolic syndrome and its related complications. However, further research in clinical trial is still necessary.

scholarly journals Efficacy of tulsi leaves and neem leaves extract in broiler production

2014 ◽  
Vol 11 (1) ◽  
pp. 1-5 ◽  
Author(s):  
S Khatun ◽  
M Mostofa ◽  
F Alom ◽  
J Uddin ◽  
MN Alam ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Control Group ◽  
Broiler Chicks ◽  
Growth Promoter ◽  
Neem Extract ◽  
Final Body Weight ◽  
Neem Leaves ◽  
Group A

The efficacy of tulsi (Ocimum sactum) and neem (Azadirachta indica) leaves extract as a growth promoter were studied in broiler. A total of 40 day-old broiler chicks were purchased from Kazi hatchery and after three days of acclimatization the chicks were randomly divided into four groups (n=10). No vaccination schedule was practiced and no antibiotic was added in ration of group A, B, C, and D respectively. Group A served control without any supplements while group B, C and D were supplemented with combination of tulsi and neem extract @ 1 ml, 2ml and 3 ml/liter of drinking water. Live body weight gain was recorded weekly up to 6th weeks and hematological studies were performed at 21st and 42nd day of experiments. At the end of 42nd day of experiment final body weight of group A, B, C and D were 1561± 12.10 g, 1698± 12.87 g, 1608± 12.04 g and 1763± 13.28 g, respectively. The birds of group D utilized their feed more efficiently among the treatment groups (p<0.05). The net body weight gain were, 1533± 11.98 g, 1443± 11.32 g and 1588± 12.10 g in treated groups compared to control group (1393± 11.07 g ) and total net profit per broiler was 19.08, 40.10, 20.68 and 45.07 Taka , respectively. Hematological parameterss (TEC, PCV, Hb and ESR) were not significantly changed among the treated and control group suggesting no side effects of herbal extracts in broiler. It can be concluded that tulsi and neem extract is economic and safe in broiler production.DOI: http://dx.doi.org/10.3329/bjvm.v11i1.17728 Bangl. J. Vet. Med. (2013). 11 (1): 1- 5

scholarly journals Effects of Neem and Nishyinda leaves supplementation on growth performance and gut biosis in broiler

2020 ◽  
Vol 6 (2) ◽  
pp. 168-175
Author(s):  
Atikur Rahaman ◽  
Md Bazlar Rashid ◽  
Md Mahmudul Hasan ◽  
Rakibul Islam ◽  
Fahima Binthe Aziz ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Growth Performance ◽  
Body Weight Gain ◽  
Health Hazard ◽  
Feed Consumption ◽  
Control Group ◽  
Broiler Chicks ◽  
Neem Leaves ◽  
Group D

This study was conducted under the Department of Physiology and Pharmacology to determine the effects of Neem and Nishyinda leaves supplementation on growth performance and GUT biosis in broilers. A total of 40 day old broiler chicks were divided into four groups A, B, C and D. Group A was considered as control, fed only with commercial broiler ration. Group B supplemented with formulation of 2 gm grinded Neem leaves, Group C with 2 gm grinded Nishyinda leaves and Group D with 1 gm grinded Neem leaves plus 1 gm grinded Nishyinda leaves per liter of water respectively. Observations were recorded for live body weight, weight gain, feed consumption, feed efficiency, hemato-biochemical parameters and GUT biosis specially the parasite of birds in six weeks. Body weights were increased significantly (p<0.05) in all treated groups in respect to the control and highest was recorded in combine Neem plus Nishyinda supplemented groups (Group D). No significant (p>0.05) differences were observed among the groups for PCV and TEC values. Hb content increased and ESR decreased significantly (p<0.05) with combine Neem plus Nishyinda supplemented group (Group D). The serum SGOT and SGPT levels were also decreased with the same kind of supplementation. In treatment group there was significantly decreased of parasitic eggs, but in case of control group no decrease of parasitic eggs. There was no significant pathological change in any internal organs of the broiler of treated groups. Best result was found in the group D. The present study reveals that combine supplementation of Neem plus Nishyinda gives better result over other groups in respect to body weight gain, feed conversion ratio, hemato-biochemical parameter and profitability without making any health hazard of the broilers. Asian J. Med. Biol. Res. June 2020, 6(2): 168-175

scholarly journals Effect of replacing maize and starch with Shoti meal (Curcuma zedoaria) in broiler

2018 ◽  
Vol 47 (1) ◽  
pp. 40-46
Author(s):  
S Datta ◽  
S Rahman ◽  
O Islam ◽  
M Hassan ◽  
M Hossain ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Feed Efficiency ◽  
Dry Matter ◽  
Body Weight Gain ◽  
Control Diet ◽  
Broiler Chicks ◽  
Curcuma Zedoaria ◽  
Physical Treatment ◽  
Dry Matter Digestibility

The effects of raw and autoclaved Shoti (Curcuma zedoaria) meal on the growth and N utilization of growing broilers were determined. Shoti from Bangladesh Agricultural University campus was collected and sorted on the basis of white color of the rhizome. Then they were chopped into pieces, sun dried and pulverized in a flour mill. Aliquot samples were autoclaved at 1200C under 15 lb of pressure per square inch for 30 min. Autoclaved samples were stored in tightly lid contained for future use. 3 One hundred -day-old Cobb 500 broiler chicks were reared on the floor and a total of 24 growing broilers, 4 birds in each group were weighed on d 15 Two broilers were placed in each cage. There were a total of four experimental and two control (positive and negative Non-protein control) diets. Diets were fed ad libitum from day 15 to 33days. Body weight, feed intake, feed refusal and fecal weights were recorded every day. Cumulative feed efficiency per bird was calculated as the ratio of weight gained to feed consumed. Dry matter of excreta was determined from the last 10 d of feeding trial by drying aliquots of every day’s droppings by drying for 6 h at 1050C. On d 19, birds were bled to death. Immediately after slaughter, the carcasses were examined systematically. The chemical composition of Shoti meal showed that it is low in protein, fat and trace minerals and therefore mainly a source of energy. The bulk of the tuber (75 percent) consists of carbohydrates, made up of 10.6% fiber, and 64.9% nitrogen free extracts (NFE). A peculiarity of Curcuma rhizome meal contains high level of ash (9.9%) on DM basis. The results showed that at day 33, the weight of the broiler chicken fed shoti 100g, 200g, 300 g and 200g (autoclaved shoti meal)/ Kg diet was significantly different from the weight of the chicken fed on a maize-soybean diet. The birds given shoti meal consumed less feed than those provided with a maize diet. Feed efficiency or weight gain to feed ration over 19-d period was the poorest when fed 300 g Shoti/ Kg diet. Intake picked up when the shoti meal was incorporated after having been autoclaved and there was slight improvement in body weight gain as compared to that of other Shoti fed group. However, feed efficiency and body weight gain as % of initial weight remained significantly lower than the maize-soybean control. The inclusion of Shoti meal generally had an adverse effect on the apparent dry matter digestibility compared with the control diets. The group fed autoclaved shoti meal had a similar effect and the true N digestibility was the lowest when compared to those of control fed on maize-soybean meal. Thus, physical treatment like using autoclave, caused further reduction of digestibility of its N. Lipid digestibility was reduced by ingestion of shoti meal. Since the diets were isocaloric, after correction for the apparent endogenous lipid losses, which were obtained from the results of non-protein control feeding, the net faecal losses was around 66.2% of the ingested lipid against control diet fed birds. Bang. J. Anim. Sci. 2018. 47 (1): 40-46

Insecticide chlorpyrifos and fungicide carbendazim, common food contaminants mixture, induce hepatic, renal, and splenic oxidative damage in female rats

2016 ◽  
Vol 36 (5) ◽  
pp. 483-493 ◽  
Author(s):  
AO Abolaji ◽  
IO Awogbindin ◽  
IA Adedara ◽  
EO Farombi
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Weight Gain ◽  
Oxidative Damage ◽  
Body Weight Gain ◽  
The Body ◽  
Female Rats ◽  
Exposure Group ◽  
Food Contaminants ◽  
Antioxidant Enzymes Activities

The fungicide carbendazim (CBZ) and insecticide chlorpyrifos (CPF) are currently applied together by farmers for the control of pests. Here, we investigated the impacts of 7 days oral co-exposure to 10 mg/kg body weight of CPF and 50 mg/kg body weight of CBZ on selected oxidative stress and antioxidant biomarkers in the liver, kidney, and spleen of female rats. The results showed that while the body weight gain and relative organ weights were not significantly affected after separate exposure to CPF and CBZ, there was a significant decrease in the body weight gain with concomitant increases in the relative kidney and spleen weights of rats treated with the mixture. Also, CPF and CBZ co-exposure significantly increased the levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), urea, and creatinine ( p < 0.05) when compared with the groups treated with CBZ or CPF alone and the control. The significant decreases in both antioxidant enzymes activities and nonenzymatic antioxidant level following individual administration of CPF and CBZ to rats were intensified in the co-exposure group ( p < 0.05). Additionally, the marked increases in the levels of oxidative stress indices in liver, kidney, and spleen of rats treated with CPF or CBZ alone were intensified in the co-exposure group ( p < 0.05). Histopathologically, co-exposure to CPF and CBZ exacerbates their individual effects on the liver, kidney, and spleen. These findings showed that co-exposure to CPF and CBZ in rats elicited more severe oxidative damage on the liver, kidney, and spleen of the rats, indicative of an additive effect compared to CPF or CBZ alone and as such, may pose a greater environmental risk to humans.

scholarly journals Antioxidant Activity of Sprouts Extracts Is Correlated with Their Anti-Obesity and Anti-Inflammatory Effects in High-Fat Diet-Fed Mice

2021 ◽  
Vol 2021 ◽  
pp. 1-13
Author(s):  
Chung Shil Kwak ◽  
Mi-Ju Kim ◽  
Sunyeong Park ◽  
In Gyu Kim
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Adipose Tissue ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Mrna Levels ◽  
Adipocyte Size ◽  
Anti Inflammatory ◽  
Visceral Adipose

Obesity is closely associated with oxidative stress and chronic inflammation leading to related metabolic diseases. Some natural extracts or polyphenols reportedly possess anti-obesity and anti-inflammatory effects as well as antioxidant activity. In this study, we assessed the correlations between the antioxidant, anti-obesity, and anti-inflammatory activities of plant extracts with potent antioxidant activity in diet-induced obese mice. Sprouts of Cedrela sinensis (CS) and Oenothera biennis L. (OB) were selected as the most potent antioxidant plant based on analysis of in vitro antioxidant activity of the extracts of ten different edible plants. C57BL/6 mice were fed with a high-fat diet (HFD) and orally treated with 50% ethanol extract of CS or OB at 50 or 100 mg/kg body weight 5 days a week for 14 weeks. Body weight gain, weight of adipose tissue, adipocyte size, and levels of lipid metabolism, inflammation, and oxidative stress markers were investigated. The CS or OB extract reduced body weight gain, visceral adipose tissue weight, adipocyte size, and plasma leptin levels, and expressions of adipogenic genes (PPARγ and fatty acid synthase) in the adipose tissue and liver of HFD-fed mice. Both extracts also reduced mRNA levels of pro-inflammatory cytokines (IL-6 and TNF-α) and oxidative stress-related genes (heme oxygenase- (HO-) 1 and p40phox). Body weight gain of mice was significantly correlated with visceral adipose tissue weight and adipocyte size. Body weight gain and adipocyte size were significantly correlated with plasma total cholesterol and 8-epi PGF2α levels, mRNA levels of leptin, HO-1, p40phox, and CD-11 in the adipose tissue, and mRNA levels of TNF-α in the adipose tissue and liver. These results suggest that the CS and OB extracts with potent antioxidant activity may inhibit fat deposition in adipose tissue and subsequent inflammation.

scholarly journals Aplikasi Teknologi Nanopartikel Perak (AgNPs) dalam Air Minum dan Bentuk Kabut terhadap Kadar Amonia Ekskreta Broiler

2019 ◽  
Vol 17 (2) ◽  
pp. 26-31
Author(s):  
Ones Putra Hulu ◽  
M Sihombing ◽  
R H Saputro ◽  
A Darmawan ◽  
Y Herbani
Keyword(s):  
Drinking Water ◽  
Body Weight ◽  
Weight Gain ◽  
Relative Humidity ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Feed Consumption ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Ammonia Content

The purpose this study was evaluate the utilization of nanoparticles silver (AgNPs) on performance and ammonia concentration in broiler excreta.  This study used a factorial completely randomized design with 3x2 treatments and 4 replicates.  First factor (AgNPs at drinking water) was P1 = 0 ppm, P2 = 2 ppm, and P3 = 3 ppm and the  second factor Q (AgNPs by misty) was Q1 = 0 ppm and Q2 = 4 ppm.  The variables measured were excreta ammonia content, feed consumption, body weight gain, feed conversion ratio, mortality, temperature and relative humidity.  The results  showed that there was no interaction between AgNPs in drinking water and AgNPs in  its mist form on ammonia content.  AgNPs 4 ppm by misty also affected reduce FCR and body weight gain at week 3, also increased FCR at fourth week.  AgNPs in drinking water affected (P < 0.05) amonia content.  AgNPs 2 ppm in drinking water reduce until 11% amonia content.  AgNPs by misty also reduced (P< 0.05) amonia content.  AgNPs 0 ppm and 4 ppm by misty not created comfort temperature and relative humidity for broiler chicks. It is concluded that the addition of silver nanoparticles reduce excreta ammonia levels of broiler chickens.

scholarly journals Dietary evaluation of starter broiler chicks fed cooked cocoyam tuber meal (xanthosoma sagitifolium) as energy source in place of maize

2020 ◽  
Vol 46 (4) ◽  
pp. 153-160
Author(s):  
B. C. Anyaegbu ◽  
D. N. Onunkwo ◽  
G. Igwe ◽  
J. Nathaniel ◽  
C. M. Nkwo
Keyword(s):  
Energy Source ◽  
Body Weight ◽  
Weight Gain ◽  
Feed Intake ◽  
Body Weight Gain ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
The Body ◽  
Broiler Chicks ◽  
Feed Conversion

Considering the inherent attributes of tannia cocoyam (Xanthosoma sagitifolium) its appropriate utilization may enhance performance of starter broiler chicks. This study was therefore carried out to determine the dietary evaluation of starter broiler chicks fed cooked cocoyam tuber meal as energy source in place of maize. The cooked cocoyam tuber meal was used to replace maize at various levels of 25%, 50%, 75% and 100% respectively for diets 2, 3, 4 and 5 to determine the best replacement level that would give optimal performance in starter broiler chicks' diets. Result of the proximate composition of cooked cocoyam tuber meal showed that it contained 13.11% moisture, 2.60% ash, 1.85% ether extract, 3.90% crude protein, 9.45% crude fibre, 70.13% nitrogen free extract and 3004.37 Kcal/kgME. 150 day old Anak broiler chicks were used for the experiment. They were divided into 5 treatment groups of 30 broiler chicks each. Each treatment group was subdivided into 3 replicates of 10 broiler chicks each and kept in deep litter. Each starter diet was fed to a group of 30 starter broilers for 4 weeks using completely randomized design. They were given feed and water adlibitum. Parameters measured include initial body weight, final body weight, body weight gain, feed intake, feed conversion ratio and economics of production. In the starter broiler feeding trial, the starter broilers on 25% cooked cocoyam meal compared favourably with the control (P> 0.05) in terms of feed intake, body weight gain and feed conversion ratio and significantly (P > 0.05) performed between than those on 100% CCYM. The cost of production (N/Kg broiler) was however cheapest for diet 2 (25% CCYM) N395 versus control diet (N494.28). It was therefore concluded that cooked cocoyam tuber meal (Xanthosoma sagitifolium) could be used in the diets of starter broiler chicks' up to 25% without affecting the body weight gain, feed intake and feed conversion ratio as indicated in this study.

scholarly journals Estimation of the relative bioavailability of several zinc sources for broilers when fed a conventional diet

2012 ◽  
Vol 28 (3) ◽  
pp. 441-453
Author(s):  
M. Sahraei ◽  
H. Janmmohamadi ◽  
A. Taghizadeh ◽  
Ali Moghadam ◽  
S.A. Rafat
Keyword(s):  
Zinc Oxide ◽  
Body Weight ◽  
Weight Gain ◽  
Zinc Sulfate ◽  
Body Weight Gain ◽  
Basal Diet ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Broiler Chicks ◽  
Feed Conversion

An experiment was conducted with 312 day-old male broiler chicks in grower phase(8-28d) to estimate the biological availability of four sources Zinc (Zn), Zinc sulfate(ZnSO4.H2O), two sources of Zinc oxide(ZnO FG1 and ZnO FG2) and Bioplex Zn. Zinc sulfate (ZnSO4.H2O) was used as the standard in the bioavailability assay. Chicks were allotted randomly to 13 dietary treatments with 6 birds per replicate and 4 replicates per treatment, that included an unsupplemented corn-soybean meal basal diet (25.50 mg of Zn/kg of DM), or the basal diet supplemented with 100, 150 or 200 mg/kg of DM as either ZnSO4.H2O (33 % Zn), Zinc oxide FG1(72%Zn), Zinc oxide FG2 (75%Zn) or Bioplex Zn (15% Zn). Dietary Zn level and source had no effect (P>0.05) of feed intake or body weight gain of chicks during first and second weeks of experimental periods, but feed conversion ratio in the first and second week and feed intake, body weight gain or feed conversion ratio in third week and total experimental periods were significant difference between treatments (P<0.05). The relative biological availability values using body weight gain were estimated to be 59, 99 or 45 for three levels of Zinc oxide FG1, 64, 78 or 31 for three levels of Zinc oxide FG2 and 151, 200 or 147 for three levels of Bioplex Zn, respectively. From the standpoint of bioavailability, Bioplex Zn was more available to broiler chicks than Zn from other sources and can be used by the feed industry as sources of supplemental Zn for broiler chickens.

Broiler chicken, Fishmeal, Infertile eggs meal, Performance, Carcass, Blood indices

2020 ◽  
Vol 47 (2) ◽  
pp. 131-143
Author(s):  
O. S. Akinola ◽  
K. A. Akintola ◽  
O. O. Oluwatosin
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Total Protein ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Serum Biochemistry ◽  
Broiler Chicks ◽  
Feed Conversion ◽  
Blood Indices ◽  
Feed Cost

Fishmeal, as animal protein source, is characterised by high cost, dwindling availability and adulteration by feed ingredient suppliers. In view of these, two hundred and ten (210) ten-day old broiler chicks were used, in 2 x 4 experimental layouts, to investigate the effect of replacing fishmeal with infertile egg meal of two forms (with or without shell) and at four graded levels (0, 30, 60 and 100%) on growth performance, haematology, serum biochemistry and carcass indices of broiler chicken. Crude protein (50.2%) and Ether extract (30.6%) were higher in infertile egg without shell (IEM) than in infertile egg withshell (IEMS) (41.36 and 23.4%). Egg forms (IEM or IEMS) did not affect (P>0.05) performance indices measured. Increasing levels of infertile egg meal reduced (P<0.05) body weight gain and final weight without affecting feed conversion ratio (FCR). The IEMS use led to reduction in feed cost (P<0.05) than in IEM. Levels of infertile egg meal reduced (P<0.05) feed cost per body weight gain with least cost at 100% replacement of fishmeal. The IEM elevated (P<0.05) serum total protein, urea and globulin but lowered serum creatinine. Increasing the levels of infertile egg meal lowered blood urea while increasingtotal protein and globulin. Inclusion of IEM at 100% improved Total protein, Albumin and Globulin. The cost of feed reduced (P<0.05) by 13.8% at 100% IEM and IEMS. It was concluded that infertile egg without shell can be used up to 100% in place of fishmeal in broiler chicken diets to reduce feeding cost and improve health without affecting the performance. 

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