scholarly journals Autolysis in Crustacean Tissues after Death: A Case Study Using the Procambarus clarkii Hepatopancreas

2021 ◽  
Vol 2021 ◽  
pp. 1-8
Author(s):  
Xiaoli Huang ◽  
Guanqing Xiong ◽  
Xia Chen ◽  
Ruisi Liu ◽  
Minghao Li ◽  
...  
Keyword(s):  
Procambarus Clarkii ◽  
Apoptotic Cell ◽  
Reference Standards ◽  
Initiation Time ◽  
Tissue Samples ◽  
Tubular Cells ◽  
Cell Counts ◽  
Tissue Changes ◽  
Mesenchyme Cells

Autolysis is an internal phenomenon following the death of an organism that leads to the degradation of tissues. In order to explore the initial stages of autolysis and attempt to establish reference standards for tissue changes after death, we studied the rapidly autolyzing tissue of the crayfish hepatopancreas. Samples from the hepatopancreas of crayfish were examined 0, 5, 10, 30, 60, and 120 minutes after death. Histological and ultrapathological examinations and evaluations and apoptotic cell counts were conducted to determine the initiation time and degree of autolysis. The results showed that autolysis in the hepatopancreas of crayfish began within 5 minutes. Initially, autolysis manifested in the swelling of hepatic tubular cells and the widening of mesenchyme. Cells undergoing autolysis showed severe organelle necrolysis. Based on these observations, tissue samples should be collected and preserved within five minutes to avoid interfering with histopathological diagnoses.

scholarly journals Notoginsenoside R1 attenuates sevoflurane-induced neurotoxicity

2020 ◽  
Vol 11 (1) ◽  
pp. 215-226
Author(s):  
Yibing Zhang ◽  
Yong Zhao ◽  
Yongwang Ran ◽  
Jianyou Guo ◽  
Haifeng Cui ◽  
...  
Keyword(s):  
Neuronal Degeneration ◽  
Apoptotic Cell ◽  
Volatile Anesthetic ◽  
Adenosine Monophosphate ◽  
Heme Oxygenase 1 ◽  
Related Factor ◽  
Sprague Dawley ◽  
Quinone Oxidoreductase ◽  
Neuroprotective Effects ◽  
Cell Counts

AbstractBackgroundSevoflurane, a volatile anesthetic, is known to induce widespread neuronal degeneration and apoptosis. Recently, the stress-inducible protein sestrin 2 and adenosine monophosphate-activated protein kinase (AMPK) have been found to regulate the levels of intracellular reactive oxygen species (ROS) and suppress oxidative stress. Notoginsenoside R1 (NGR1), a saponin isolated from Panax notoginseng, has been shown to exert neuroprotective effects. The effects of NGR1 against neurotoxicity induced by sevoflurane were assessed.MethodsSprague-Dawley rat pups on postnatal day 7 (PD7) were exposed to sevoflurane (3%) anesthesia for 6 h. NGR1 at doses of 12.5, 25, or 50 mg/kg body weight was orally administered to pups from PD2 to PD7.ResultsPretreatment with NGR1 attenuated sevoflurane-induced generation of ROS and reduced apoptotic cell counts. Western blotting revealed decreased cleaved caspase 3 and Bad and Bax pro-apoptotic protein expression. NGR1 substantially upregulated nuclear factor erythroid 2-related factor 2 (Nrf2) expression along with increased heme oxygenase-1 (HO-1) and NAD(P)H quinone oxidoreductase-1 levels, suggesting Nrf2 signaling activation. Enhanced sestrin-2 and phosphorylated AMPK expression were noticed following NGR1 pretreatment.ConclusionThis study revealed the neuroprotective effects of NGR1 through effective suppression of apoptosis and ROS via regulation of apoptotic proteins and activation of Nrf2/HO-1 and sestrin 2/AMPK signaling cascades.

scholarly journals Pathological Relationship between Intracellular Superoxide Metabolism and p53 Signaling in Mice

2021 ◽  
Vol 22 (7) ◽  
pp. 3548
Author(s):  
Kenji Watanabe ◽  
Shuichi Shibuya ◽  
Yusuke Ozawa ◽  
Toshihiko Toda ◽  
Takahiko Shimizu
Keyword(s):  
Apoptotic Cell ◽  
Oxygen Species ◽  
Double Knockout ◽  
In Vivo Experiments ◽  
P53 Signaling ◽  
Tissue Degeneration ◽  
Age Related ◽  
Tissue Changes ◽  
P53 Activation

Intracellular superoxide dismutases (SODs) maintain tissue homeostasis via superoxide metabolism. We previously reported that intracellular reactive oxygen species (ROS), including superoxide accumulation caused by cytoplasmic SOD (SOD1) or mitochondrial SOD (SOD2) insufficiency, induced p53 activation in cells. SOD1 loss also induced several age-related pathological changes associated with increased oxidative molecules in mice. To evaluate the contribution of p53 activation for SOD1 knockout (KO) (Sod1−/−) mice, we generated SOD1 and p53 KO (double-knockout (DKO)) mice. DKO fibroblasts showed increased cell viability with decreased apoptosis compared with Sod1−/− fibroblasts. In vivo experiments revealed that p53 insufficiency was not a great contributor to aging-like tissue changes but accelerated tumorigenesis in Sod1−/− mice. Furthermore, p53 loss failed to improve dilated cardiomyopathy or the survival in heart-specific SOD2 conditional KO mice. These data indicated that p53 regulated ROS-mediated apoptotic cell death and tumorigenesis but not ROS-mediated tissue degeneration in SOD-deficient models.

Prognostic significance of bcl-xL gene expression and apoptotic cell counts in follicular lymphoma

Blood ◽  
2004 ◽  
Vol 103 (2) ◽  
pp. 695-697 ◽  
Author(s):  
Wei-Li Zhao ◽  
Marjan Ertault Daneshpouy ◽  
Nicolas Mounier ◽  
Josette Brière ◽  
Christophe Leboeuf ◽  
...  
Keyword(s):  
Gene Expression ◽  
Follicular Lymphoma ◽  
Apoptotic Cell ◽  
International Prognostic Index ◽  
Prognostic Significance ◽  
Prognostic Index ◽  
Pcr Analysis ◽  
Survival Times ◽  
Lymphoma Cells ◽  
Cell Counts

Abstract bcl-xL, a member of the Bcl-2 family, exerts an antiapoptotic effect on lymphocytes. To assess its clinical significance in patients with follicular lymphoma, realtime quantitative reverse transcription–polymerase chain reaction (RT-PCR) analysis of bcl-xL gene expression was investigated in whole lymph node sections and laser-microdissected lymphoma cells of 27 patients. Compared with 10 patients with reactive follicular hyperplasia, the bcl-xL gene was overexpressed in patients with follicular lymphoma at a higher level in microdissected lymphoma cells. The bcl-xL gene level correlated with the number of apoptotic lymphoma cells labeled by terminal deoxytransferase-catalyzed DNA nick-end labeling (TUNEL) assays (r = -0.7736). Clinically, a high bcl-xL level was significantly associated with multiple sites of extranodal involvement (P = .0020), elevated lactate dehydrogenase level (P = .0478), and an International Prognostic Index indicating high risk (P = .0235). Moreover, bcl-xL gene overexpression was linked to short overall survival times (P = .0129). The value of bcl-xL gene expression as a prognostic marker in follicular lymphoma should thus be considered.

Longitudinal characterization of a model of chronic allergic lung inflammation in mice using imaging, functional and immunological methods

2013 ◽  
Vol 125 (12) ◽  
pp. 555-564 ◽  
Author(s):  
Kumar Changani ◽  
Catherine Pereira ◽  
Simon Young ◽  
Robert Shaw ◽  
Simon P. Campbell ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Lung Inflammation ◽  
Allergic Inflammation ◽  
Treated Group ◽  
Similar Rate ◽  
Immunological Methods ◽  
Tissue Density ◽  
Cell Counts ◽  
Rate Of Increase ◽  
Tissue Changes

The present study investigated the role that imaging could have for assessing lung inflammation in a mouse model of HDM (house dust mite)-provoked allergic inflammation. Inflammation is usually assessed using terminal procedures such as BAL (bronchoalveolar lavage) and histopathology; however, MRI (magnetic resonance imaging) and CT (computed tomography) methods have the potential to allow longitudinal, repeated study of individual animals. Female BALB/c mice were administered daily either saline, or a solution of mixed HDM proteins sufficient to deliver a dose of 12 or 25 μg total HDM protein±budesonide (1 mg/kg of body weight, during weeks 5–7) for 7 weeks. AHR (airway hyper-responsiveness) and IgE measurements were taken on weeks 3, 5 and 7. Following imaging sessions at weeks 3, 5 and 7 lungs were prepared for histology. BAL samples were taken at week 7 and lungs prepared for histology. MRI showed a gradual weekly increase in LTI (lung tissue intensity) in animals treated with HDM compared with control. The 25 μg HDM group showed a continual significant increase in LTI between weeks 3 and 7, the 12 μg HDM-treated group showed a similar rate of increase, and plateaued by week 5. A corresponding increase in AHR, cell counts and IgE were observed. CT showed significant increases in lung tissue density from week 1 of HDM exposure and this was maintained throughout the 7 weeks. Budesonide treatment reversed the increase in tissue density. MRI and CT therefore provide non-invasive sensitive methods for longitudinally assessing lung inflammation. Lung tissue changes could be compared directly with the classical functional and inflammatory readouts, allowing more accurate assessments to be made within each animal and providing a clinically translatable approach.

scholarly journals WRF Model Simulations of the Influence of the Athabasca Oil Sands Development on Convective Storms

2018 ◽  
Vol 22 (3) ◽  
pp. 1-25 ◽  
Author(s):  
Daniel Brown ◽  
Gerhard Reuter
Keyword(s):  
Land Surface ◽  
Oil Sands ◽  
Waste Heat ◽  
Model Simulation ◽  
Wrf Model ◽  
Initiation Time ◽  
Athabasca Oil Sands ◽  
Model Simulations ◽  
Surface Disturbance

Abstract The Athabasca oil sands development has created a land surface disturbance of almost 900 km2 in northeastern Alberta. Both through industrial processes and the removal of boreal forest vegetation, this surface disturbance impacts meteorology in the vicinity by releasing waste heat, raising the surface temperature, and lowering the surface humidity. To investigate the effects of the Athabasca oil sands development on thunderstorm intensity, initiation time, and duration, the Weather Research and Forecasting (WRF) Model was employed to simulate the effect of the surface disturbance on atmospheric conditions on 10 case study days. The results suggested the oil sands surface disturbance was not associated with substantial increases in thunderstorm intensity on any of the case study days. On two case study days, however, the WRF Model simulations differed substantially from the observed meteorological conditions and only approached the observations when the oil sands surface disturbance was included in the model simulation. Including the oil sands surface disturbance in the model simulations resulted in thunderstorm initiation about 2 h earlier and increased thunderstorm duration. Data from commercial aircraft showed that the 850–500-mb temperature difference was greater than 30°C (very unstable) only on these 2 days. Such cases are sufficiently rare that they are not expected to affect the overall thunderstorm climatology. Still, in these very unstable cases, the oil sands development appears to have a significant effect on thunderstorm initiation time and duration.

Neuroserpin reduces cerebral infarct volume and protects neurons from ischemia-induced apoptosis

Blood ◽  
2000 ◽  
Vol 96 (2) ◽  
pp. 569-576 ◽  
Author(s):  
Manuel Yepes ◽  
Maria Sandkvist ◽  
Mike K. K. Wong ◽  
Timothy A. Coleman ◽  
Elizabeth Smith ◽  
...  
Keyword(s):  
Basement Membrane ◽  
Stroke Volume ◽  
Plasminogen Activator ◽  
Apoptotic Cell ◽  
Ischemia Reperfusion ◽  
Tissue Type ◽  
Ischemic Penumbra ◽  
Cell Counts ◽  
Therapeutic Benefits ◽  
Type Plasminogen Activator

Abstract Neuroserpin, a recently identified inhibitor of tissue-type plasminogen activator (tPA), is primarily localized to neurons within the central nervous system, where it is thought to regulate tPA activity. In the present study neuroserpin expression and its potential therapeutic benefits were examined in a rat model of stroke. Neuroserpin expression increased in neurons surrounding the ischemic core (ischemic penumbra) within 6 hours of occlusion of the middle cerebral artery and remained elevated during the first week after the ischemic insult. Injection of neuroserpin directly into the brain immediately after infarct reduced stroke volume by 64% at 72 hours compared with control animals. In untreated animals both tPA and urokinase-type plasminogen activator (uPA) activity was significantly increased within the region of infarct by 6 hours after reperfusion. Activity of tPA then decreased to control levels by 72 hours, whereas uPA activity continued to rise and was dramatically increased by 72 hours. Both tPA and uPA activity were significantly reduced in neuroserpin-treated animals. Immunohistochemical staining of basement membrane laminin with a monoclonal antibody directed toward a cryptic epitope suggested that proteolysis of the basement membrane occurred as early as 10 minutes after reperfusion and that intracerebral administration of neuroserpin significantly reduced this proteolysis. Neuroserpin also decreased apoptotic cell counts in the ischemic penumbra by more than 50%. Thus, neuroserpin may be a naturally occurring neuroprotective proteinase inhibitor, whose therapeutic administration decreases stroke volume most likely by inhibiting proteinase activity and subsequent apoptosis associated with focal cerebral ischemia/reperfusion.

scholarly journals A 20-Year Climatology of Nocturnal Convection Initiation over the Central and Southern Great Plains during the Warm Season

2017 ◽  
Vol 145 (5) ◽  
pp. 1615-1639 ◽  
Author(s):  
Dylan W. Reif ◽  
Howard B. Bluestein
Keyword(s):  
Great Plains ◽  
Warm Season ◽  
Thunderstorm Activity ◽  
Surface Boundary ◽  
Initiation Time ◽  
Southern Great Plains ◽  
Surface Data ◽  
Convection Initiation ◽  
Storm Characteristics

Abstract A nocturnal maximum in rainfall and thunderstorm activity over the central Great Plains has been widely documented, but the mechanisms for the development of thunderstorms over that region at night are still not well understood. Elevated convection above a surface frontal boundary is one explanation, but this study shows that many thunderstorms form at night without the presence of an elevated frontal inversion or nearby surface boundary. This study documents convection initiation (CI) events at night over the central Great Plains from 1996 to 2015 during the months of April–July. Storm characteristics such as storm type, linear system orientation, initiation time and location, and others were documented. Once all of the cases were documented, surface data were examined to locate any nearby surface boundaries. The event’s initiation location relative to these boundaries (if a boundary existed) was documented. Two main initiation locations relative to a surface boundary were identified: on a surface boundary and on the cold side of a surface boundary; CI events also occur without any nearby surface boundary. There are many differences among the different nocturnal CI modes. For example, there appear to be two main peaks of initiation time at night: one early at night and one later at night. The later peak is likely due to the events that form without a nearby surface boundary. Finally, a case study of three nocturnal CI events that occurred during the Plains Elevated Convection At Night (PECAN) field project when there was no nearby surface boundary is discussed.

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