scholarly journals Impact of Plasma-Activated Water (PAW) on Seed Germination of Soybean

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Rajesh Prakash Guragain ◽  
Suman Prakash Pradhan ◽  
Hom Bahadur Baniya ◽  
Bishnu Prasad Pandey ◽  
Niroj Basnet ◽  
...  
Keyword(s):  
Water Treatment ◽  
Seed Germination ◽  
Dissolved Oxygen ◽  
Seedling Growth ◽  
Treatment Time ◽  
Germination Rate ◽  
Physical Parameters ◽  
Oxygen Conductivity ◽  
Normal Water ◽  
Plasma Activated Water

The present study reports the generation of plasma-activated water (PAW) using dielectric barrier discharge (DBD), its physicochemical properties, and its potential impact on the seed germination and seedling growth of soybean. The results revealed significant changes in physical parameters, such as pH, total dissolved solids, total suspended solids, turbidity, conductivity, dissolved oxygen, and chemical parameters, such as calcium, chromium, sodium, manganese, nitrate, nitrites, phosphorus, and sulfur and biological parameter such as E. coli in water after plasma treatment. The concentration of dissolved oxygen, conductivity, nitrate, nitrite, and sulfur was increased with an increase in water treatment time, and the amounts of the other analyzed parameters decreased with the increase in water treatment time. The effects of untreated water and plasma-activated water treated for 20 minutes on soybean germination and growth were studied. The germination rate was found to be higher with plasma-treated water. Shoot lengths, seedlings length, vigor index, and germination rates were increased as compared to those germinated by normal water irrigation. The seedlings irrigated with PAW responded to the abundance of nitrogen by producing intensely green leaves because of their increased chlorophyll a as compared to seedlings irrigated with normal water. However, the content of chlorophyll b and carotenoids was found to decrease in the case of seedlings irrigated with PAW. Based on this report, we conclude that PAW could be used to substantially enhance seed germination and seedling growth.

scholarly journals The Effect of Oleander (Nerium oleander L.) Extracts on Seed Germination and Seedling Growth of Four Pistacia Species

2019 ◽  
Vol 7 (2) ◽  
pp. 286
Author(s):  
Yusuf Nikpeyma ◽  
Mehmet Hakkı Alma ◽  
Ferudun Koçer
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Treatment Time ◽  
Germination Rate ◽  
Nerium Oleander ◽  
Plant Parts ◽  
Positive Effects ◽  
Flower Stem ◽  
Pistacia Terebinthus ◽  
Pistacia Species

In this study, the effects of the extracts obtained from flower, stem, leaf, branch and their mixture of Nerium oleander L. on the seed germination and seedling growth of four Pistacia species (Pistacia terebinthus L., P. vera L., P. khinjuk Stocks., P. atlantica Desf. and P. terebinthus L.) were investigated. Five hundred grams of N. oleander was taken from the plant parts and the extracts were mixed with distilled water (1.5 L). The extracts were applied to Pistacia species for 24 and 48 hours. The results showed that the extract had positive effects on their germination and growths of the species mentioned above. The highest germination rate for all the Pistacia species was obtained from the extracts of flower, while the lowest germination rate was recorded in the mixture of all parts of N. oleander mentioned above. Moreover, the treatment time was found to reduce the germination ratio. It was also found that the extracts from the stem of N. oleander were the most effective on the stem height of the Pistacia species studied. It was followed by the extracts from branches, leaves and flower, respectively. Furthermore, the findings indicated that the extracts from the stem of N. oleander had pronounced effect on the stem diameter of the Pistacia species studied. It was followed by the extracts from flower, leaves, and branches, respectively. In general, the effects of the extracts from several parts of N. oleander on the germination and other growing parameters were found to be almost comparable to those of synthetic promoter, Gibberellic Acid (GA3).

scholarly journals Impacts of different salt source and concentrations on germination and seedling growth of many pumpkin seeds used as rootstoch in Iran

Genetika ◽  
2012 ◽  
Vol 44 (2) ◽  
pp. 235-250 ◽  
Author(s):  
Ahmad Dadashpour
Keyword(s):  
Electrical Conductivity ◽  
Seed Germination ◽  
Seedling Growth ◽  
Root Length ◽  
Germination Rate ◽  
Shoot Length ◽  
Root Weight ◽  
Pumpkin Seeds ◽  
High Concentrations ◽  
Shoot Weight

The effects of different salt sources (C Cl2, NaCl, and KCl) and concentrations, as measured by electrical conductivity, (0, "control", 1, 3, 5, 7 and 9 dS m-1) on seed germination and seedling growth of ?Ferro?, ?Obez?, ?RS 841? and ?Strong Tosa F1? pumpkin varieties used as rootstock were investigated in this study. The results showed that germination rate, root length, shoot length, fresh root weight, dry root weight, fresh shoot weight and dry shoot weights tend to decrease when the electrical conductivity of the solution is higher than 5 dS m-1, independent of salt sources and in all of the varieties. Three days after seeding, a germination ratio of 5 % was obtained from RS 841 variety in all salt source and concentrations, while a germination ratio over 50 % was obtained in ?Strong Tosa? variety for the same conditions except CaCl2 salt source. Nevertheless, seeds germinated in medium having high concentrations of CaCl2 had lower germination rate and poor seedling growth, compared to media having the same concentrations of NaCl and KCl. It was concluded that all of the varieties studied were more sensitive to the concentrations prepared using CaCl2 than that of the KCl, and NaCl.

scholarly journals Elucidation of seed dormancy and phytohormones by germination after exogenous foliage whey application

1970 ◽  
pp. 08-14
Author(s):  
Jadhav Rajesh K, Pranay Mulam, Khot Vidhesh
Keyword(s):  
Seed Germination ◽  
Seed Dormancy ◽  
Seedling Growth ◽  
Rapid Growth ◽  
Germination Rate ◽  
Exogenous Application ◽  
Paper Towel ◽  
Leguminous Seeds ◽  
Growth Of Seedlings ◽  
Leaf Juice

A study was conducted with an aim of achieving 100% seed germination and to evaluate the rate of dormancy upon exogenous application of the deproteinised leaf juice (DPJ) in various leguminous and non leguminous seeds. DPJ if inducing retardation of dormancy, the activity of enzyme was analysed to prove the presence of phytohormones. DPJ from the three non leguminous foliages of brinjal, radddish and dasheen were prepared by green crop fractionation. The seed germination results obtained by the paper towel method and the activity of enzyme protease in DPJ of radish and Colocasia leaves separately. There was variation in germination rate by different DPJ influence. Some seeds showed fast germination while some germinated late. Few seeds were not germinated by control. In some seeds, DPJ decreased the rate of germination while on the contrary there was rapid growth of seedlings by the potentiality of DPJ. DPJ found mutagenic by its influencing inhibitory expression in seedling growth in some cases. The enzyme protease released by phytohormone gibberellin during the seed germination. Positive glyoxlic test indicated presence of auxins in whey.

scholarly journals ​Influence of Temperature and Salinity Stress on Seed Germination and Seedling Growth of Soybean (Glycine max L.)

2021 ◽  
Author(s):  
Mustafa Çirka ◽  
Ali Rahmi Kaya ◽  
Tamer Eryiğit
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Germination Rate ◽  
Petri Dish ◽  
Negative Effects ◽  
Randomized Design ◽  
Salt Level ◽  
Glycine Max L ◽  
Early Seedling ◽  
Lower Temperature

Background: The high salt level of a germinating environment can lead to reduced, delayed, and even complete inhibition of germination and seeding growth due to osmotic action and/or ion toxicity. Based on this viewpoint, the aim of this study was to investigate germination temperature and salinity effects on germination and early seedling growth of soybean, which can be sown as first and second crops. Methods: Soybean seeds were subjected to NaCl induced saline germinating media prepared in petri dishes under two different germination temperatures (20±2 and 25±2oC). Thirty sterilized seeds per petri dish were sown in ten salt treatments (0, 100, 150, 250, 750, 1000, 2250, 5000, 7500 and 10000 ppm NaCl L-1). The study was carried out according to the completely randomized design with four replications. Result: Lower temperature promoted seed germination, while the high temperature significantly inhibited the seed germination at all NaCl doses tested. As a result, 25oC temperatures, which can only be measured at the time of the second sowing, have been found to negatively affect germination and also increase the negative effects of salt. Due to the moderate tolerance of soybeans to salt stress, the germination rate was positively affected up to 750 ppm NaCl L-1 dose and resulted in severe reductions in subsequent doses. Also, the tolerance of soybean was negatively influenced by the interaction of temperature and NaCl concentration.

scholarly journals Transcriptome Analysis Revealed the Regulation of Gibberellin and the Establishment of Photosynthetic System Promote Rapid Seed Germination and Early Growth of Seedling in Pearl Millet

2020 ◽  
Author(s):  
Bingchao Wu ◽  
Min Sun ◽  
Huan Zhang ◽  
Dan Yang ◽  
Chuang Lin ◽  
...  
Keyword(s):  
Seed Germination ◽  
Pearl Millet ◽  
Seedling Growth ◽  
Germination Rate ◽  
Expression Patterns ◽  
Early Growth ◽  
High Yield ◽  
Maintenance Cost ◽  
Key Genes ◽  
Pearl Millet Seeds

Abstract Background：Seed germination is the most important stage for the formation of a new plant. This process starts when the dry seeds begin to absorb water and ends when the radicles sticks out. The germination rate of different plant seeds varies differently. Most energy plants that usually grow on marginal land, the rapid germination of seeds is more conducive to its superiority in competition with surrounding plants, which is also the guarantee of normal plant development and high yield. Pearl millet is an important cereal crop that shares widespread applications in the world. It has the advantages of fast growth, high yield, and low maintenance cost. It can also be used to extract bioethanol to solve the increasingly prominent energy problems. Previous germination experiments are the evidence of very fast seed germination rate of pearl millet, but the molecular mechanisms behind it is still unclear.Results: Through the germination test and the measurement of the germs and radicles length of the seedlings, we found that pearl millet seeds germinated very quickly after 24 hours of swelling of the dry seeds. By using transcriptome sequencing technology, we characterized the gene expression patterns of dry seeds, water imbibed seeds, germs and radicles of seedlings, and found the more DEGs in radicles than germs. Further analysis showed that different genome clusters function specifically at different tissues and time periods. WGCNA and KEGG enrichment analysis showed that that many genes that positively regulate plant growth and development are highly enriched and expressed, especially the gibberellin signaling pathway that can promote seed germination. We speculated that the activation of these key genes promotes the germination of pearl millet seeds and the growth of seedlings. To verify this inference, we measured the content of the main effect hormone gibberellin and found that the gibberellin content after seed imbibition rose sharply and remained at a high level.Conclusions：This study explored the expression patterns of genes involved in pearl millet growth from the germination of dry seeds to the early growth stages. Also identifies the key genes involved in the regulation of seed germination and seedling growth. The activation of key genes in these pathways may contribute to the rapid germination and growth of seeds and seedlings in pearl millet. These results provide new insights to solve the problem for the plants with slow seed germination and seedling growth.

scholarly journals The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity Stress

2012 ◽  
Vol 76 (1) ◽  
pp. 119-126 ◽  
Author(s):  
Maryam Haghighi ◽  
Zahra Afifipour ◽  
Maryam Mozafarian
Keyword(s):  
Seed Germination ◽  
Salinity Stress ◽  
Seedling Growth ◽  
Germination Rate ◽  
Germination Percentage ◽  
Percentage Germination ◽  
Tomato Seeds ◽  
Germination Characteristics ◽  
Salinity Levels ◽  
Mean Germination Time

The Alleviation Effect of Silicon on Seed Germination and Seedling Growth of Tomato Under Salinity StressThis study was conducted to evaluate the effectiveness of silicon (Si) application under salinity levels on seed germination and growth characteristics of tomato seeds. A laboratory experiment was performed on completely randomized design with two levels of salinity (25 and 50 mM NaCl) and 2 concentration of Si (1 and 2 mM) with 4 replications. Germination percentage, germination rate, seedling shoot and root length, fresh and dry weight of seedling and mean germination time was measured. Seed germination ofLycopersicon esculentumL. was significantly affected by salinity levels, Si and their interaction. Germination characteristics of tomato seeds decreased drastically by increasing NaCl concentrations. However, 1 mM Si had positive effects on seed germination characteristics and improved germination percentage, germination rate and mean germination time. Si alleviated the harmful effect of salinity stress on tomato seed germination at almost all germination characteristics.

scholarly journals Utilization of Grape Fruit Waste-based Substrates for Seed Germination and Seedling Growth of Lemon Basil

2017 ◽  
Vol 27 (4) ◽  
pp. 523-529 ◽  
Author(s):  
Mohammed El-Sayed El-Mahrouk ◽  
Yaser Hassan Dewir ◽  
Salah El-Hendawy
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Growth Parameters ◽  
Low Cost ◽  
Germination Rate ◽  
Germination Percentage ◽  
Waste Recycling ◽  
Grape Fruit ◽  
Fruit Waste ◽  
One To One

Grape (Vitis vinifera) waste management is a major problem in juice production, but it could be transformed into a major opportunity if the waste was recycled and used as a nursery growing medium. The aim of this study was to evaluate the suitability of four composts based on squeezed grape fruit waste (SGFW), mixed with coir or vermiculite in a one-to-one ratio by volume to form 13 growing media, for seed germination and seedling growth of ‘Mrs. Burns’ lemon basil (Ocimum basilicum var. citriodora). The final germination percentage (FGP), corrected germination rate index (CGRI), survival percentage, and seedling growth of ‘Mrs. Burns’ lemon basil were the variables measured. Pure SGFW reduced seed germination and seedling growth. The medium combining pure SGFW with vermiculite in a one-to-one ratio by volume was optimal for seed germination and seedling growth; in this medium the highest FGP, CGRI, survival rate, and growth parameters were recorded. The negative effects of pure SGFW composts were eliminated by mixing all composts with coir or vermiculite. These waste recycling media are low-cost products that can be beneficially used in nurseries on a commercial scale.

scholarly journals Seed Germination and Early Seedling Growth of Barley at Negative Water Potentials

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 671
Author(s):  
Tim L. Springer ◽  
Dolores W. Mornhinweg
Keyword(s):  
Seed Germination ◽  
Water Potential ◽  
Seedling Growth ◽  
Germination Rate ◽  
Moisture Stress ◽  
Potential Treatment ◽  
Line Treatment ◽  
Plant Materials ◽  
Early Seedling ◽  
Line P

The impacts of climate change may increase the duration and frequency of droughts, which would have deleterious effects on crop establishment. The objectives of this study were to determine the effects of moisture stress on seed germination and seedling growth of six winter barley (Hordeum vulgare) lines and discuss how the data are used to select plant materials for rapid germination. Twenty-five seeds of each line were germinated in water of potentials of −2.0, −1.6, −1.2, −0.8, −0.4, and 0 MPa for 4- and 7-days. The experimental design was a factorial arrangement of treatments (barley lines and water potential treatments) in a randomized block replicated four times and repeated twice. The 4- and 7-day percentage seed germination varied with line (p < 0.01), water potential treatment (p < 0.01), and line × treatment interactions (p < 0.01). The seed germination rate varied with water potential treatment (p < 0.01), and line × treatment interactions (p < 0.01). The data indicated that enough variation was present to effectively select and breed cultivars for improved germination at a negative water potential. Studying seed germination under moisture stress is the first step for developing an effected selection pressure for identifying plant materials with rapid seed germination.

scholarly journals Responses of Seed Germination, Seedling Growth, and Seed Yield Traits to Seed Pretreatment in Maize (Zea maysL.)

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
Yu Tian ◽  
Bo Guan ◽  
Daowei Zhou ◽  
Junbao Yu ◽  
Guangdi Li ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seed Yield ◽  
Seedling Growth ◽  
Germination Rate ◽  
Seed Priming ◽  
Grain Weight ◽  
Shoot Biomass ◽  
Control Group ◽  
Yield Traits ◽  
Seed Pretreatment

A series of seed priming experiments were conducted to test the effects of different pretreatment methods to seed germination, seedling growth, and seed yield traits in maize (Zea maysL.). Results indicated that the seeds primed by gibberellins (GA), NaCl, and polyethylene glycol (PEG) reagents showed a higher imbibitions rate compared to those primed with water. The final germination percentage and germination rate varied with different reagents significantly (P<0.05). The recommended prime reagents were GA at 10 mg/L, NaCl at 50 mM, and PEG at 15% on account of germination experiment. 15% PEG priming reagent increased shoot and root biomass of maize seedling. The shoot biomass of seedlings after presoaking the seeds with NaCl reagent was significantly higher than the seedlings without priming treatment. No significant differences of plant height, leaf number, and hundred-grain weight were observed between control group and priming treatments. Presoaking with water, NaCl (50 mM), or PEG (15%) significantly increased the hundred-grain weight of maize. Therefore, seed pretreatment is proved to be an effective technique to improve the germination performance, seedling growth, and seed yield of maize. However, when compared with the two methods, if immediate sowing is possible, presoaking is recommended to harvest better benefits compared to priming method.

scholarly journals Effects of Plasma Focus on Seed Germination and Seedling Growth of 14 Thai Rice Varieties

2021 ◽  
Vol 18 (14) ◽  
Author(s):  
Vichai PURIPUNYAVANICH ◽  
Arlee TAMMAN ◽  
Piyanuch ORPONG ◽  
Roppon PICHA ◽  
Mayuree LIMTIYAYOTHIN ◽  
...  
Keyword(s):  
Seed Germination ◽  
Seedling Growth ◽  
Plasma Focus ◽  
Germination Rate ◽  
Growth Reduction ◽  
Rice Varieties ◽  
X Ray ◽  
Plasma Irradiation ◽  
Germination And Growth ◽  
Storage Energy

Thailand Plasma Focus 2 (TPF-2) is operated at 2.16 kJ of storage energy with argon as a filled gas and can emit sharp x-ray pulses to plant cells in nanosecond (10-9 s) duration. The effects of plasma focus x-ray on seed germination and seedling growth of 14 Thai rice varieties treated under 10 conditions; 0 (control), 2, 4, 6, 8, 10, 12, 14, 16, and 18 plasma focus shots, were investigated. The results show that the germination rates and growth reduction rates were significantly different among the rice varieties. The germination rate of 10 varieties (Jek Chuey, KDML105, LPT123, PTT1, RD41, RD47, RD49, RD67, RD79, and SPR2) decreased when treated with a high number of plasma focus shots. LD50 and GR50 values were calculated, except for KTH17, RD1, RD7, and RD31 whose germination only slightly decreased, or remained constant, even after they were treated by 18 plasma focus shots. HIGHLIGHTS Effects of plasma focus irradiation on germination and growth were assessed on 14 Thai rice varieties  Shoot and root lengths of rice seedlings were measured 10 days after of plasma treatment Plasma focus was operated at 12 kV and the number of plasma focus shots was varied from 0 (control) to 18 shots Sensitivity to plasma irradiation was found to differ among rice seeds tested GRAPHICAL ABSTRACT

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