scholarly journals Associations of Pulmonary Fibrosis with Peripheral Blood Th1/Th2 Cell Imbalance and EBF3 Gene Methylation in Uygur Pigeon Breeder’s Lung Patients

2018 ◽  
Vol 47 (3) ◽  
pp. 1141-1151 ◽  
Author(s):  
Chao Wu ◽  
Zujin Luo ◽  
Baosen Pang ◽  
Wenyi Wang ◽  
Mingqin Deng ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Pulmonary Fibrosis ◽  
Mrna Expression ◽  
Peripheral Blood ◽  
Enzyme Linked Immunosorbent Assay ◽  
Gene Methylation ◽  
Control Groups ◽  
Th2 Cell ◽  
Breeding History ◽  
Pigeon Breeding

Background/Aims Pigeon breeder’s lung (PBL) results from Th1/Th2 cell imbalance. B cells inhibit the immune activity of Th1, and EBF3 is a key B cell factor. This study explored the relationship between EBF3 and Th1/Th2 imbalance in chronic PBL cases complicated with pulmonary fibrosis (PF). Methods Twenty Uygur PBL+PF patients, 20 pigeon breeders without PBL or PF, and 20 healthy individuals without pigeon breeding history constituted the patient I, negative control, and normal control groups, respectively. Peripheral blood specimens and case backgrounds were collected between June 2016 and March 2017. EBF3 gene methylation was analyzed by matrix assisted laser desorption ionization-time of flight mass spectrometry. To compare different mechanisms of PF progression in PBL, samples from 20 Uygur PBL patients without PF (at acute and sub-acute stages) were collected between October 2017 and February 2018, constituting the patient II group. EBF3 mRNA expression was evaluated by real-time polymerase chain reaction. IFN-γ, IL-4 and IL-10 expression and Th1/Th2 imbalance in PBL were evaluated by enzyme-linked immunosorbent assay and flow cytometry. Results CpG-2 and general methylation rates in the patient I group were lower than those in the control groups (P˂0.017). The level of EBF3 mRNA expression in the patient I group was significantly higher than that in any other group. Compared with the control groups, the patient I group showed a significantly higher level of IL-4, whereas the patient II group showed a significantly lower level. IL-10 was also expressed more highly in the patient I group than in any other group (P< 0.01). Flow cytometry showed INF-γ dominance (Th1 cytokine) in PBL at the acute/sub-acute stage and IL-4 dominance (Th2 cytokine) at the chronic stage after PF occurred. The general methylation rate was negatively correlated with the mRNA level, with the latter being positively correlated with the IL-10 level and number of pigeons bred in the past 3 months. IL-4 expression was negatively correlated with INF-γ but positively correlated with PF area and duration of pigeon breeding history. Conclusions After PF occurs in chronic PBL, the inflammation type changes from Th1 dominance to Th2 dominance. During PBL development, IL-10 increases before IL-4 does, which may be associated with EBF3 hypomethylation and the involvement of B lymphocytes.

scholarly journals Identification of molecular signatures of cystic fibrosis disease status with plasma-based functional genomics

2019 ◽  
Vol 51 (1) ◽  
pp. 27-41 ◽  
Author(s):  
Hara Levy ◽  
Shuang Jia ◽  
Amy Pan ◽  
Xi Zhang ◽  
Mary Kaldunski ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Flow Cytometry ◽  
Functional Genomics ◽  
Treatment Response ◽  
Peripheral Blood ◽  
Disease Status ◽  
Enzyme Linked Immunosorbent Assay ◽  
Molecular Signatures ◽  
Healthy Controls ◽  
Plasma Samples

Although cystic fibrosis (CF) is attributed to dysfunction of a single gene, the relationships between the abnormal gene product and the development of inflammation and progression of lung disease are not fully understood, which limits our ability to predict an individual patient’s clinical course and treatment response. To better understand CF progression, we characterized the molecular signatures of CF disease status with plasma-based functional genomics. Peripheral blood mononuclear cells (PBMCs) from healthy donors were cultured with plasma samples from CF patients ( n = 103) and unrelated, healthy controls ( n = 31). Gene expression levels were measured with an Affymetrix microarray (GeneChip Human Genome U133 Plus 2.0). Peripheral blood samples from a subset of the CF patients ( n = 40) were immunophenotyped by flow cytometry, and the data were compared with historical data for age-matched healthy controls ( n = 351). Plasma samples from another subset of CF patients ( n = 56) and healthy controls ( n = 16) were analyzed by multiplex enzyme-linked immunosorbent assay (ELISA) for numerous cytokines and chemokines. Principal component analysis and hierarchical clustering of induced transcriptional data revealed disease-specific plasma-induced PBMC profiles. Among 1,094 differentially expressed probe sets, 51 genes were associated with pancreatic sufficient status, and 224 genes were associated with infection with Pseudomonas aeruginosa. The flow cytometry and ELISA data confirmed that various immune modulators are relevant contributors to the CF molecular signature. This study provides strong evidence for distinct molecular signatures among CF patients. An understanding of these molecular signatures may lead to unique molecular markers that will enable more personalized prognoses, individualized treatment plans, and rapid monitoring of treatment response.

scholarly journals Heat stress combined with lipopolysaccharide alter the activity and superficial molecules of peripheral monocytes

2019 ◽  
Vol 33 ◽  
pp. 205873841982889
Author(s):  
Jiajing Luo ◽  
Yi Chen ◽  
Chengjia Ding ◽  
Jialing Qiu ◽  
Yulan Chen ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Heat Stress ◽  
Western Blot ◽  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Heat Stroke ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Rt Pcr ◽  
Tnf Α

The purpose of this study was to focus on the underlying relationship between the hyperactivity for the peripheral monocytes and heat stroke by investigating the inflammatory oxidative activity of and the expression of superficial molecules. Peripheral blood samples were collected from 10 healthy adult volunteers. Human blood monocytes were isolated by density gradient centrifugation and sequent adherent culture. The objectives were divided into four groups: 43°C heat stress combined with lipopolysaccharide (LPS) group, 43°C heat stress group, LPS group, and control group. There were 10 cases in each group. An enzyme-linked immunosorbent assay (ELISA) test was used to measure the concentrations of supernatant inflammatory mediators (tumor necrosis factor alpha (TNF-α), interleukin-1β (IL-1β) and interleukin-10 (IL-10)). After loaded by 2,7-Dichlorodi-hydrofluorescein-diacetate (DCFHDA) fluorescent probe, intracellular reactive oxygen species (ROS) levels were determined by a flow cytometry. After fluorescent microspheres incubation, the phagocytosis of monocytes was observed under a fluorescent microscope. Respectively, the flow cytometry and Western blot were used to evaluate the level of triggering receptor expressed on myeloid cells-1 (TREM-1) and Toll-like receptor-4 (TLR-4) on the monocytes. Furthermore, the mRNA expression of TREM-1 and TLR-4 was detected by real-time polymerase chain reaction (RT-PCR). The heat stress combined with LPS stimulation promoted the peripheral monocytes to produce inflammatory mediators (TNF-α, IL-1β, and IL-10) and release ROS. Otherwise, such complex strike significantly suppressed the phagocytic activity of monocytes in peripheral blood. Moreover, the expression of TREM-1, TLR-4 and CD86 was measured by the flow cytometry on peripheral monocytes which were respectively promoted by the union of heat stress and LPS. The results of Western blot and RT-PCR demonstrated the similar kinetics on these superficial molecules (TREM-1, TLR-4, and CD86) stimulated by the combination of heat stress and LPS. The underlying mechanism of the dysfunction for the peripheral monocytes may be related to the abnormal expression of superficial molecules TREM-1, TLR-4, and CD86 on the monocytes induced by heat stress and LPS.

scholarly journals Effect of Traditional Chinese Medicine on Inflammatory Mediators in Pediatric Asthma

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Hui Du ◽  
Yonghong Wang ◽  
Yumin Shi ◽  
Jian Yu ◽  
Wen Sun ◽  
...  
Keyword(s):  
Chinese Medicine ◽  
Traditional Chinese Medicine ◽  
Mrna Expression ◽  
Inflammatory Mediators ◽  
Peripheral Blood ◽  
Transforming Growth Factor ◽  
Pediatric Asthma ◽  
Enzyme Linked Immunosorbent Assay ◽  
Significant Difference ◽  
Before And After

Objective. To observe the effects of empirical prescriptions of traditional Chinese medicine (TCM) on inflammatory mediators in pediatric asthma and to explore the underlying molecular mechanism in the treatment of asthma.Methods. A total of 182 children with asthma were randomly placed into either the TCM group (n=97) or the salbutamol and montelukast (SM) group (n=85). Patients in the TCM group were treated with a series of empirical prescriptions of TCM, while those in the SM group received salbutamol and montelukast. Both groups received their respective treatment for 12 weeks. There were 35 patients in TCM group and 34 patients in SM group providing venous blood. Real-time PCR was used to determine the mRNA expression levels of interleukin- (IL-) 10, IL-17, matrix metalloproteinase-9 (MMP-9), and transforming growth factorβ1 (TGF-β1) in peripheral blood mononuclear cells before and after treatment. Enzyme-linked immunosorbent assay was used to measure the levels of IL-10, IL-17, MMP-9, and TGF-β1 in peripheral blood before and after treatment.Results. The mRNA expression of TGF-β1 in the SM group was downregulated (P=0.00) after treatment. No significant differences were found between the TCM group and the SM group after treatment (P>0.05). In the TCM group, the levels of IL-10, IL-17, and MMP-9 significantly decreased after treatment (P=0.01, 0.04, and 0.03, resp.). In the SM group, IL-17, MMP-9, and TGF-β1 levels significantly decreased after treatment (P=0.00, 0.03, and 0.00, resp.). There was no significant difference between the two groups regarding the levels of IL-10, IL-17, TGF-β1, and MMP-9 (P>0.05). The difference of the level of IL-17 was negatively correlated with the change of C-ACT score in TCM group and SM group.Conclusion. TCM has a regulatory effect on the balance of some inflammatory mediators in pediatric asthma.

scholarly journals ASSESSMENT OF ENDOTHELIAL DYSFUNCTION IN PREGNANT WOMEN WITH OBESITY AND PREECLAMPSIA

2021 ◽  
Vol 74 (8) ◽  
pp. 1905-1909
Author(s):  
Marta M. Zelinka-Khobzey ◽  
Kostiantyn V. Tarasenko ◽  
Tetiana V. Mamontova
Keyword(s):  
Flow Cytometry ◽  
Growth Factor ◽  
Endothelial Dysfunction ◽  
Blood Serum ◽  
Pregnant Women ◽  
Peripheral Blood ◽  
Enzyme Linked Immunosorbent Assay ◽  
Endothelial Microparticles ◽  
Vascular Growth ◽  
Vascular Growth Factor

The aim: To assess the values of endothelial vascular growth factor (VEGF) in blood serum and circulating endothelial microparticles CD32+CD40+ in the peripheral blood of pregnant women depending on the severity of obesity and presence of preeclampsia. Materials and methods: the study included 122 pregnant women divided into groups in accordance with their height and weight parameters and presence of preeclampsia. We studied the serum VEGF concentration by enzyme-linked immunosorbent assay, carried out the count of CD32+CD40+ circulating endothelial microparticles in the peripheral blood by using flow cytometry. Results: It has been found out the serum VEGF concentration in pregnant women with obesity decreases with rising level of obesity and the preeclampsia manifestation. In contrast to the decrease in this marker, there is an increase in the number of circulating endothelial microparticles CD32+CD40+ in the peripheral blood of pregnant women with obesity and preeclampsia. This pattern of these indicators points out the presence of endothelial dysfunction, which may contribute to occurrence of preeclampsia in pregnant women with concomitant obesity. Conclusions: The indicators of VEGF concentration and the count of circulating endothelial microparticles CD32+CD40+ in the blood serum can serve as reliable markers for evaluating the severity of endothelial dysfunction in pregnant women with concomitant obesity and preeclampsia.

Study on the Change of Iron Transporter Expression in k562 Cells Apoptosis Induced by Artesunate

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 5019-5019
Author(s):  
Liu Xiaoli ◽  
Junmei Gong ◽  
Qingfeng Du ◽  
Rong Li ◽  
Song Zhang ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Mrna Expression ◽  
Antitumor Effect ◽  
Untreated Group ◽  
Flow Cytometry Analysis ◽  
Rt Pcr ◽  
High Concentration ◽  
Control Groups ◽  
Iron Transporter ◽  
Positive Rate

Abstract Background and objective The antitumor effect of artemisinin was widely reported in vivo and ex vivo, but the possible mechanisms have yet been poorly understood. As is the anti-malaria effect of artemisinin, the tumour cells have the richer iron than the normal cells, which maybe result to be sensitive to artemisinins. Moreover, it is a very interesting question whether artesininin and its derivates can affect the iron metabolism of tumor cells and finally result to the antitumor effect. So in this study, we investigate the expression change of iron transporter including TfR1 (transferrin receptor-1, CD71), DMT1(Divalent metal transporter 1) and FPN1(Ferroportin 1) in the wild-type K562 cell(K562-W) and imatinib-resistant K562 cell(K562-R) treated with artesunates. Methods The K562-W cells and K562-R cells were treated with 4×10−5 mol/L, 2×10−4 mol/L and 1×10−3 mol/L artesunate for 24, 48 and 72 hours respectively. The control groups include the untreated group and the group treated with desferrioxamine(20 umol/L). The different expressions of TfR1, DMT1 and FPN1 between the groups treated with artesunates and the control groups were analyzed by Semi-quantitative RT-PCR, Western-Blotting or flow cytometry. Apoptosis was measured by Annexin V-PI and Hochest 33258 staining. Results The artesunate can induce K562-W and K562-R cells apoptosis in time- and concentration-dependent manner. When K562-W cells treated with 4×10−5 mol/L, 2×10−4 mol/L and 1×10−3 mol/L of artesunate for 48 hours, the mRNA expression of TfR1 is lower in artesunate groups than in the untreated groups. DMT1 mRNA expression in low concentration of artesunate and desferrioxamine group is lower than untreated group, and there is no expression of DMT1 mRNA in middle and high concentration artesunate group. The expression of FPN1 mRNA in artesunate group and desferrioxamine group is lower than the untreared group. The positive rate of CD71 is lower in artesunate groups than untreated group by flow cytometry analysis. The expression of DMT1 protein in desferrioxamine group is lower than untreated group and no expression of DMT1 protein in artesunate groups by Western blot. The TfR1 mRNA expression of K562-R cells treated with 4×10−5mol/L, 2×10−4mol/L and 1×10−3mol/L artesunate for 48 hours is lower in artesunate groups than untreated group by Semi-quantity RT-PCR. DMT1 mRNA expression in low concentration artesunate group is lower than untreated group, and there is no expression of +IRE-DMT1 mRNA in middle and high concentration artesunate group. The expression of FPN1 mRNA in artesunate group is lower than that in the untreated group. The positive rate of CD71 is lower in artesunate groups than untreated group by flow cytometry analysis. There is no expression of DMT1 protein in middle concentration artesunate groups by Western blot. Conclusions Artesunate may reduce TfR1 and DMT1 expression in mRNA and/or protein level in K562-W and K562-R and lead to reduce the iron intake, iron transportation from the endosome to the cytoplasm and iron utilization in the mitochondria. Furthermore, Artesunate also reduce the expression of FPN1 mRNA to inhibit the outflow of iron. In addition to the generation of free radicals inducing to cell death, Artesunate can inhibit cell proliferation and induce cell apoptosis by the regulation of iron transporter.

T-lymphocyte subsets in peripheral blood and lung lavage in idiopathic pulmonary fibrosis and sarcoidosis: Analysis by monoclonal antibodies and flow cytometry

1982 ◽  
Vol 25 (1) ◽  
pp. 11-20 ◽  
Author(s):  
Leo C. Ginns ◽  
Paul D. Goldenheim ◽  
Robert C. Burton ◽  
Robert B. Colvin ◽  
Lawrence G. Miller ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Monoclonal Antibodies ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
T Lymphocyte ◽  
Peripheral Blood ◽  
Lymphocyte Subsets ◽  
Lung Lavage ◽  
T Lymphocyte Subsets

scholarly journals IL-9 blockade attenuates inflammation in a murine model of methicillin-resistant Staphylococcus aureus pneumonia

2020 ◽  
Vol 52 (2) ◽  
pp. 133-140
Author(s):  
Weihua Xu ◽  
Keyin Tian ◽  
Xiaoshuang Li ◽  
Shihai Zhang
Keyword(s):  
Staphylococcus Aureus ◽  
Flow Cytometry ◽  
Peripheral Blood ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Neutralizing Antibody ◽  
Lavage Fluid ◽  
Cell Counting ◽  
Enzyme Linked Immunosorbent Assay ◽  
Methicillin Resistant ◽  
Th9 Cells

Abstract Methicillin-resistant Staphylococcus aureus (MRSA) is an important etiology of pneumonia. Interleukin (IL)-9 is a T helper 9 (Th9) cytokine and participates in the pathogenesis of infectious diseases. Here, we investigated the role of IL-9 by using an MRSA pneumonia animal model. The BALB/c mice underwent nasal inhalation with an ST239 MRSA strain to establish the mouse model of MRSA pneumonia, and a subset of mice were intravenously injected with IL-9 neutralizing antibody or immunoglobulin (Ig) G. At 3 and 8 days postinfection, the peripheral blood, bronchioalveolar lavage fluid (BALF), and lung tissues were collected. The frequencies of Th9 cells and the levels of cytokines in peripheral blood, BALF, and lung tissues were determined by flow cytometry and enzyme-linked immunosorbent assay (ELISA), respectively. The colony counts of MRSA in BALF and lung tissue were detected. The lung pathological changes were examined using hematoxylin and eosin staining. Data from flow cytometry, qRT-PCR, and ELISA showed that MRSA-infected mice exhibited higher frequency of Th9 cells and higher IL-9 mRNA and protein levels in the peripheral blood, BALF, and lung tissues of mice. In contrast, the neutralization of IL-9 abrogated MRSA inoculation-induced Th9 cell generation and IL-9 production in BALF and lung tissues. Furthermore, bacterial counting and histological examination showed that the numbers of bacteria in BALF and lungs and the lung pathological scores induced by MRSA inoculation were attenuated by the neutralization of IL-9. Moreover, cell counting and ELISA results demonstrated that IL-9 neutralization diminished the MRSA inoculation-induced count of neutrophils and macrophages and levels of pro-inflammatory cytokines in BALF. Collectively, IL-9 neutralization attenuated inflammation of MRSA pneumonia by regulating Th9/IL-9 expression.

Autologous Mesenchymal Stem Cell Therapy for Idiopathic Pulmonary Fibrosis and Comprehensive Assessment of Circulating Immune Populations Cells

2021 ◽  
Author(s):  
Elena Atanasova ◽  
Dragana Milosevic ◽  
Svetlana Bornschlegl ◽  
Karen Krucker ◽  
Eapen Jacob ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Stem Cell ◽  
Idiopathic Pulmonary Fibrosis ◽  
Pulmonary Fibrosis ◽  
Osteogenic Differentiation ◽  
Peripheral Blood ◽  
T Cell Subsets ◽  
Age And Gender ◽  
Self Renewal ◽  
And Gender

Abstract BackgroundIdiopathic pulmonary fibrosis (IPF) is a chronic, progressive pulmonary disease characterized by aberrant tissue remodeling, formation of scar tissue within the lungs and continuous loss of lung function. The areas of fibrosis seen in lungs of IPF patients share many features with normal aging lung with cellular senescence being one. The contribution of the immune system to the etiology of IPF remains poorly understood. Evidence obtained from animal models and human studies suggests that innate and adaptive immune processes can orchestrate existing fibrotic responses. Currently, there is only modestly effective pharmacotherapy for IPF. Mesenchymal stem cells (MSCs)-based therapies have emerged as a potential option treatment of IPF. This study explores the possibility of using autologous MSCs as an IPF therapy and present an attempt to determine if the disease occurring in the lungs can be reflected on peripheral blood immune status. MethodsComprehensive characterization of autologous adipose derived MSCs (aMSCs) from five IPF patients and five age and gender matched Healthy Controls (HC) was done using flow cytometry, Droplet Digital PCR (ddPCR), Multiplex Luminex xMAP technology and confocal microscopy. For assessing the self renewal capacity and osteogenic differentiation IncuCyte Live Cell Imaging technology was used. Multi-parameter quantitative flow cytometry of un-manipulated whole blood of another group of 15 IPF patients and 87 (30 age and gender matched) HC was used to analyze 110 peripheral phenotypes.ResultsThere are no differences between autologous aMSCs from IPF patients and HC in their stem cell properties, self renewal capacity, plasticity for osteogenic differentiation, secretome content, cell cycle inhibitor marker levels and mitochondrial health. IPF patients had altered peripheral blood immunophenotype including reduced B cells subsets, increased T cell subsets, and increased granulocytes among others demonstrating clear and significant differences.ConclusionsOur results indicate that there is no difference in aMSCs properties from IPF patients and HC, suggesting that autologous aMSCs may be an acceptable option for IPF therapy.Characterization of peripheral immune phenotype may be a valuable indicator for successful therapy, and for potentially staging the disease.

A P-Selectin/CD62P Monoclonal Antibody (LYP-20), in Tandem with Flow Cytometry, Detects in vivo Activated Circulating Rat Platelets in Severe Vascular Trauma

1994 ◽  
Vol 72 (05) ◽  
pp. 745-749 ◽  
Author(s):  
Elza Chignier ◽  
Maud Parise ◽  
Lilian McGregor ◽  
Caroline Delabre ◽  
Sylvie Faucompret ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Flow Cytometry ◽  
Platelet Activation ◽  
Peripheral Blood ◽  
Vascular Trauma ◽  
Activated Platelets ◽  
Group I ◽  
Group Ii ◽  
Rat Platelets

SummaryP-selectin, also known as CD62P, GMP140 or PADGEM, is present in platelet a-granules and endothelial cell Weibel-Palade bodies and is very rapidly expressed on the surface of these cells on activation. In this study, an anti P-selectin monoclonal antibody (LYP20) was used, in tandem with flow cytometry, to identify activated platelets at the site of induced vascular trauma or in peripheral blood. Moreover, electron microscopy was performed to characterize sites of vascular trauma and quantify the number of adhering platelets. The same induced vascular trauma was observed to result into animals responding in 2 different ways (Group I, Group II) following the degree of platelet activation. Five rats, out of 14 with induced vascular trauma, had more than half of their circulating platelets expressing P-selectin when drawn at the site of the trauma (67.4% ± 3.44) or in peripheral blood (78.5% ± 2.5) (Group I). In the remaining 9 animals a much smaller proportion of circulating platelets expressed P-selectin when assayed from trauma sites (18% ± 3.34) or in peripheral blood (18.0% ± 4.30) (Group II). Enhanced P-selectin expression by circulating platelets in Group I, compared to Group II, appears to be linked to the degree of activated platelets adhering at sites of trauma (171 ± 15 × 103 platelets versus 48 ± 31 × 103 platelets per mm2). In the 5 control animals, that were not operated on, platelets expressing P-selectin when drawn at the site of a mock trauma (7.0% ± 1.84) or in the peripheral blood (11.2% ± 3.30) showed little activation. In addition, no platelet adhesion was seen on the vascular bed of these animals. Results from this study show that analysis of P-selectin (CD62P) expression, in circulating platelets, is a valuable and rapid marker of platelet activation following severe vascular trauma induced in rats. However, activated platelets were not detected to the same extent in the peripheral blood of all animals having undergone vascular trauma. It is conceivable that platelets, depending on the degree of activation, may be actively sequestered in organs and prevented from circulating. Alternatively, P-selectin may be rapidly endocytosed, or not expressed, by activated circulating platelets depending on the type of agonists implicated in vivo activation.

Quantitative mRNA expression of PROK2, DUSP6, and WDR11 in peripheral blood as diagnostic criteria for central hypogonadism

2020 ◽  
Author(s):  
Anna Loktionova ◽  
Irena Ilovayskaya ◽  
Lidia Nefedova
Keyword(s):  
Mrna Expression ◽  
Diagnostic Criteria ◽  
Peripheral Blood
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