scholarly journals A complex influence of chromium and zinc citrates on antioxidant defense system in rats’ organism with an experimentally induced diabetes mellitus

2017 ◽  
Author(s):  
O. M. Slivinska ◽  
R. J. Iskra
Keyword(s):  
Diabetes Mellitus ◽  
Diabetes Prevention ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Antioxidant Defense System ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Research Groups ◽  
Group 3 ◽  
Group 2

Introduction. Since the diabetes mellitus is a genetically predetermined disease, preventive measures to postpone the onset of the disease or to avert its appearance are especially important for people from risk group. That is why it is so important to evaluate role of some microelements in diabetes prevention. In particular, it is known that zinc plays an important role in insulin synthesis, accumulation and release by β-cells in pancreas, while its synergist chromium activates insulin receptors on cell membranes.The aim of the study – to establish the effect of chromium and zinc citrates on the functioning of antioxidant system and on the content of lipid peroxidation products in rats’ blood and tissues under the condition of experimental streptozotocin-induced diabetes mellitus.Methods of the research. Experiments were conducted on white laboratory rats which were divided into three groups: 1 – the control group, 2, 3 – research groups. Rats from the 1 and 2 groups received only basic ration, while chromium and zinc citrates solutions were added to the water of animals from the group 3 in 25 mcg Cr/kg and 50 mg Zn/kg of body weight dosage. An experimental diabetes mellitus (EDM) was induced in animals from 2 and 3 research groups on the 25th day of the experiment, after 24-hour fasting, by intraperitoneal injection of Streptozotocin in 45 mg/kg bodyweight dosage. Materials for the research were rats’ blood and tissues that were received after decapitation. The content of lipid hydroperoxides in blood and tissues was determined by a method that requires protein sedimentation by a trichloroacetic acid (TCA) and further addition of ammonium thiocyanate to the solution. The MDA content was measured by means of a colored reaction of malondialdehyde with the thiobarbituric acid. The activity of superoxide dismutase (SOD, EC 1.1.15.1.) was determined by a method where nitrotetrazolium is reduced by superoxide radicals. Glutathione peroxidase activity (GP, EC 1.11.1.9.) was measured by the speed of reduced glutathione oxidation. The activity of catalase (EC 1.11.1.6.) was measured by hydrogen peroxide ability to form a stable colored complex with molybdenum salts. The activity of glutathione reductase (GR, EC 1.6.4.2) was measured by a speed of glutathione reduction in the presence of NADPH. The content of reduced glutathione was gauged by the level of thionitrophenol anion formation as a result of SH-groups of glutathione and 5.5-dithiobis-(2-nitrobenzoic acid) reaction.Results and Discussion. It was determined during the research that lipid hydroperoxides (LHP) and MDA contents in rats’ blood, skeletal muscles and pancreas were increased, while the activity of antioxidant defense system enzymes and reduced glutathione content were decreased under the condition of streptozotocin-induced DM. Under the condition of combined chromium and zinc citrates addition to the diet of rats from group 3 we observed the credible decrease of lipid hydroperoxides content, while the increased activity of antioxidant enzymes compared to those indexes from group 2.Conclusions. The received results indicate the normalization of lipids peroxidation processes and antioxidant defense under the influence of chromium and zinc citrates in animals with hyperglycemia. The further research of antioxidant properties of organic compounds of these microelements will let to develop medical remedies for diabetes prevention and treatment. 

scholarly journals Corrective effect of red wine concentrate enriched with natural complex of polyphenols on activity of antioxidant defense enzymes in cardiac muscle under experimental diabetes mellitus

2021 ◽  
Vol 15 (1) ◽  
pp. 37-48
Author(s):  
D. Yu. Hertsyk ◽  
◽  
M. V. Sabadashka ◽  
L. V. Kaprelyants ◽  
N. O. Sybirna ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Superoxide Dismutase ◽  
Antioxidant Defense ◽  
Red Wine ◽  
Experimental Diabetes ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Experimental Diabetes Mellitus ◽  
Corrective Effect ◽  
Antioxidant Defense Enzymes

Background. Diabetes mellitus is accompanied by oxidative-nitrative stress, which is caused both by an increased generation of the Reactive Oxygen Species and the Reactive Nitrogen Species under the conditions of this pathology and by the disorders of the antioxidant defense system, especially its enzymatic part. This leads alterations in the morpho-functional state of cells, organs, and the whole organism. Free radicals are involved in the destruction of pancreatic cells in type 1 diabetes mellitus, which leads to even greater inhibition of insulin secretion, worsening of the course of the disease and occurrence of diabetic complications. The complications of diabetes mellitus include often diagnosed cardiovascular diseases. Therefore, the use of treatments characterized not only by hypoglycemic properties for normalization of blood sugar level in diabetes mellitus, but also by antioxidant properties for normalization of oxidative/antioxidant balance of the organism in the studied pathology can be promising. Scientists pay a great attention to the study of substances with such properties, especially natural phenolic compounds of grape, which are also characterized by immunomodulatory properties. Materials and Methods. The study was performed on cardiac tissues of control rats, control animals treated with wine polyphenolic complex concentrate, animals with streptozotocin-induced diabetes mellitus and animals with experimental diabetes mellitus treated with a concentrate of wine polyphenolic complex. The activity of catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase were detected to examine the corrective effect of the concentrate of red wine natural polyphenolic comp­lex on the state of the enzymatic part of the antioxidant defense system. Results. The results have shown the normalization of activity of catalase, superoxide dismutase, and changes in the activity of enzymes of glutathione cycle after oral administration of polyphenolic complex concentrate for 14 days to rats with streptozo­tocin-induced diabetes mellitus. Conclusions. The results confirm a hypothesis about the antioxidant effect of the studied concentrate and the ability of natural polyphenolic complexes to serve as the basis for new drugs for treatment of diabetes-induced disorders.

scholarly journals Biochemical evaluation of excisional wound healing in rats with diabetes mellitus after using different methods of wound closure

2017 ◽  
Author(s):  
Ye. O. Loza ◽  
M. I. Marushchak
Keyword(s):  
Diabetes Mellitus ◽  
Wound Healing ◽  
Free Radical ◽  
Antioxidant Defense ◽  
Free Radical Oxidation ◽  
Antioxidant Defense System ◽  
Male Rats ◽  
Defense System ◽  
Radical Oxidation ◽  
Oxidation Processes

Introduction. Wound healing according to modern concepts is a coordinated process that passes through certain stages with the participation of different cells and products of its vital functions that regulate the healing process. It is well known that in patients with diabetes there is a violation of the process of wound healing, although until now, all the features of diabetes underlying such an inclination have not been fully understood.The aim of the study – to identify the features of oxidative stress on the background of diabetes mellitus in the rat's homogenate when different methods of closing wounds had been used. Research Methods. The experiment was conducted on 60 male rats weighing 280–320 g, which pre-simulated diabetes mellitus and surgical wounds. To detect the activation of free radical oxidation processes, the content of active forms of oxygen (AFC), lipid hydroperoxides (HPL), diene conjugates (DK) and trienic conjugates (TC) and oxidation modifications of proteins (OMP370 and OMP430). To study the antioxidant defense system, activity of superoxide dismutase was determined. Also, the activity of catalase and sulfhydryl groups (SH-groups) was determined.Results and Discussion. The research conducted by us showed that during the wounded process, on the background of diabetes, the activity of free radical oxidation processes increased. However, in the animal skin homogenates, which are labeled "Dermabond", all indices are significantly lower than in the group of animals that have been sewed over the course of all research periods. In the early stages of scar formation (3 days), an increase in the activity of the antioxidant defense system in both groups was observed. At the same time, in the experimental group of animals, which imposed the nodal seams, the activity of SOD and catalase significantly exceeded the skin index of animals, which applied glue and made (199.81±7.59) UM and (175.02±8.31) cat/kg, respectively.Conclusion. The use of skin glue reduces the intensity of the course of free radical oxidation in the cells of the scar tissue of the postoperative wound in conditions of diabetes mellitus in comparison with the imposition of nodal sutures on it: in the skin of animals, which is applied to the "Dermabond" glue, all indicators are significantly lower than in the group of animals, which imposed seam joints, throughout all research periods. 

Lipid Peroxidation and Antioxidant Defense System Changes in Mongoloids with Type 1 Diabetes Mellitus

2016 ◽  
Vol 100 ◽  
pp. S175-S176
Author(s):  
Marina A. Darenskaya ◽  
Lyudmila A. Grebenkina ◽  
Maria I. Dolgikh ◽  
Larisa V. Natyaganova ◽  
Svetlana V. Gnusina ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Lipid Peroxidation ◽  
Type 1 Diabetes ◽  
Type 1 Diabetes Mellitus ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Defense System ◽  
System Changes

Effect ofBauhinia racemosaStem Bark on N-nitrosodiethylamine-Induced Hepatocarcinogenesis in Rats

2007 ◽  
Vol 35 (01) ◽  
pp. 103-114 ◽  
Author(s):  
Ramanathan Sambath Kumar ◽  
Rajagopal Shanmuga Sunderam ◽  
Thangavel Sivakumar ◽  
Palanivel Sivakumar ◽  
Raman Sureshkumar ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Uric Acid ◽  
Antioxidant Defense ◽  
Methanol Extract ◽  
Reduced Glutathione ◽  
Antioxidant Defense System ◽  
Serum Enzymes ◽  
Nodule Development ◽  
Albino Rats ◽  
Defense System

The aim of this study is to investigate the antioxidant defense system induced by the methanol extract of Bauhinia racemosa L.(MEBR) against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis in Wister albino rats. The effects of MEBR on surface visible macroscopic (Morphometry) liver lesions (neoplastic nodules) and the levels of serum enzymes, lipid peroxidation and antioxidants were evaluated in NDEA-induced hepatocarcinogenesis in rats.In rats treated, with NDEA, significantly elevated levels of serum enzymes (SGOT, SGPT and ALP), bilirubin and decreased levels of protein and uric acid were observed. Significantly elevated amount of malondialdehyde (MDA), the end product of lipidperoxidation, indicated higher levels of lipid peroxidation, which was accompanied by significantly decreased levels of antioxidants like vitamin C, vitamin E, reduced glutathione (GSH), superoxide dismutase (SOD) and catalase (CAT). Administration of MEBR was able to suppress nodule development/hepatocellular lesion formation in rats. The extract treatment increases in antioxidant levels and dramatic decreases in lipid peroxidation levels. MEBR also produced a protective effect by decreasing the level of serum enzymes, bilirubin and increased the protein and uric acid levels. The results suggest that MEBR exert chemopreventive effects by suppressing nodule development and decreasing lipid peroxidation and enhancing the levels of antioxidants in NDEA carcinogenesis by reducing the formation of free radicals.

scholarly journals Influence of cadmium loading on the state of the antioxidant system in the organism of bulls

2016 ◽  
Vol 24 (1) ◽  
pp. 96-102 ◽  
Author(s):  
B. V. Gutyj ◽  
S. D. Mursjka ◽  
D. F. Hufrij ◽  
I. I. Hariv ◽  
N. D. Levkivska ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Cadmium Chloride ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Young Cattle

This article presents the results of research on the influence of cadmium loading on the state level of enzymatic and non-enzymatic antioxidant links of the antioxidant defense system of the organisms of young cattle, such as the activity of catalase, superoxide dismutase, glutathione peroxidase, glutathione levels, selenium, vitamins A and E. It was found that feeding bull calves with cadmium chloride at doses of 0.03 and 0.05 mg/kg of body weight helped to reduce both the enzymatic and non-enzymatic link of antioxidant protection (superoxide dismutase 31%, catalase 13%, glutathione peroxidase 23%, reduced glutathione 10%, vitamin A 28%, vitamin E 31%, selenium 20%). Toxic effects of cadmium promotes change in steady-state concentrations of radical metabolites О2–,ОН˙, НО2˙, which, in turn, trigger the process of lipid peroxidation. The lowest level of indicators of antioxidant defense system in the blood of young cattle was registered on the sixteenth and twenty-fourth days of the experiment, which is associated with increased activation of lipid peroxidation and the disturbaance of the balance between the antioxidant system and lipid peroxidation intensity. The activity of the antioxidant defense system in the blood was different for calves fed with cadmium chloride at doses of 0.03 and 0.05 mg/kg of animal mass. The more cadmium chloride in the feed, the lower the activity of the antioxidant defense system of the calves’ organisms was registered. Thus cadmium chloride depresses the antioxidant defense system, which specifically involves lowering the activity of enzymatic links (catalase, superoxide dismutase, glutathione peroxidase) and non-enzymatic links (reduced glutathione, selenium, vitamins A and E).

scholarly journals The influence of oxidative stress on the state of the antioxidant defense system in the organism of rats

2016 ◽  
Vol 7 (1) ◽  
pp. 8-12
Author(s):  
T. V. Martyshuk
Keyword(s):  
Oxidative Stress ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Experimental Group ◽  
Free Radical Lipid Oxidation ◽  
Free Radical Lipid ◽  
Excessive Accumulation

This article presents the results of research on the influence of oxidative stress on the intensity of the process of lipid peroxidation and the activity of the glutathione system of antioxidant defense in the organisms of rats. Intramuscular injection of 50% solution of tetrachloromethane at a dose of 0.25 ml per100 gof body weight to rats from the experimental group causes activation of the process of free radical lipid oxidation with excessive accumulation of intermediate anf final products of lipid peroxidation. The research results indicate that the development of oxidative stress leads to significant and probable acceleration of the formation and accumulation in the plasma of the rats, in all stages of the experiment, of lipid hydroperoxides and malonic dialdehyde. The highest level of hydroperoxides of lipids in the blood plasma of rats under oxidative stress was on the second day of the experiment, where it was 843 unE/ml, whereas in the control this index was 0.245 unE/ml. During the research into the content of malondialdehyde it was found that in the experimental group of animals it was 2.03 times higher than in the control group on the 5th day. On the 10th and 14th days of the experiment we observed a slight reduction in the levels of lipid hydroperoxides and malondialdehyde. The development of oxidative stress also leads to inhibition of the glutathione system of antioxidant defense in the rats’ organism. This shows the low activity of glutathione peroxidase and the low level of restored glutathione in the blood of the rats from the experimental group. On the 5th day of experiment the activity of glutathione peroxidase and restored glutathione level in the blood of the rats which were injected with carbon tetrachloride was at its lowest, compared with the control these indices decreased respectively by 53% and 51%. On the 10th and 14th days of the experiment the activity of glutathione peroxidase and restored glutathione level in the blood of the rats from the experimental group were slightly increased, but compared to the control they were still significantly lower. Significant disturbances were found in the oxidation-antioxidant balance of the animals under oxidative stress, which is characterized by the activation of the processes of free radical lipid oxidation with excessive accumulation of intermediate and final products and the inhibition of the antioxidant defense system.

scholarly journals Antioxidant status of piglets under nitrate-nitrite load and the action of corrective factors

2020 ◽  
Vol 3 (2) ◽  
pp. 53-59
Author(s):  
K. Y. Leskiv ◽  
B. V. Gutyj ◽  
D. F. Gufriy ◽  
V. I. Khalak ◽  
O. G. Demchuk
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Diene Conjugates ◽  
Peroxidation Processes

The article presents the results of research on the state of the antioxidant defense system of piglets under nitrate-nitrite toxicosis and the action of corrective factors (methionine, phenarone and methyphene). Nitrate-nitrite load in piglets caused inhibition of the activity of enzymes of the antioxidant defense system, that indicates a decrease in the activity of superoxide dismutase, catalase and glutathione peroxidase in the serum of testing animals. The lowest activity of antioxidant enzymes under chronic nitrate-nitrite toxicosis was on the 60th day of the experiment in the serum of piglets of the control group, where accordingly the activity of superoxide dismutase decreased by 14 %, catalase activity – by 18 %, glutathione peroxidase activity – by 12 %. Lipid peroxidation processes are activated in the blood of animals, namely the level of lipid hydroperoxides and diene conjugates increases under the conditions of experimental nitrate-nitrite load. It should be noted that the highest level of lipid peroxidation products in the serum of piglets with chronic nitrate-nitrite toxicosis was on the 60 th day of the testing, and accordingly the content of lipid hydroperoxides increased by 67 % and diene conjugates – by 33 %. Administration of methionine, phenarone and methyphene under nitrate load contributed to the activation of the antioxidant defense system compared to animals in the control group. The use of antioxidant drugs in sick piglets also contributed to the inhibition of lipid peroxidation, as indicated by a decrease in the content of lipid hydroperoxides and diene conjugates in the serum of experimental group. More pronounced antioxidant effect was shown by administrating methyphene. The mechanism of action of phenarone and methyphene is associated with the direct effect of its components on the inhibition of the absorption of metabolites that have a toxic effect on cell membranes of animals. As a result of the adsorbing action of zeolite, which is part of the drugs, in the digestive tract there is a decrease in the concentration of substances that can be substrates for lipid peroxidation, as well as removal of toxic metabolites in the blood, which are prooxidants. This process occurs by osmosis and diffusion of these substances through the capillaries of the microvilli of the small intestine and their subsequent fixation on the sorbent granules.

Physiological Reactivity and Antioxidant Defense System of the Animal Organism Induced by Germanium, Chromium, and Selenium “Nanoaquacitrates” Soil-Remediating Activity of Agroecosystems and Chernozem Fertility Restoration Using Low-Carbon Technologies

2015 ◽  
Vol 2 (2) ◽  
pp. 50-55 ◽  
Author(s):  
O. Dolaychuk ◽  
R. Fedoruk ◽  
S. Kropyvka
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Physiological Effect ◽  
Female Rats ◽  
Physiological Reactivity ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Infant Rats ◽  
Old Rats

Aim. To study the state of physiological reactivity of the organism and blood antioxidant defense system of female rats and their offspring under prolonged feeding with “nanoaquacitrates” of germanium, chromium, and selenium. Methods. To accomplish the stated objective we carried out physiological and biochemical studies, using colori- metric methods to study the content of glycoproteins and separate monosaccharides of their carbohydrate com- ponents. The state of antioxidant defense system was studied by the intensity of lipid peroxidation that was also determined using the colorimetric methods based on the content of lipid hydroperoxides and TBA-active products in blood. Results. The intergroup differences in the content of glycoproteins and their carbohydrate components in the blood of female rats and their offspring of both experimental groups have been observed compared against the control. This may indicate approximately the same integrated biological effect of chromium, selenium, germanium in the second group, fed with chromium and selenium citrates along with the drinking water, calculated as 50 μg per 1 kg of body weight (b.w.) for Cr and Se, and germanium citrate – 15 μg Ge per 1 kg of b.w. The fi rst group served as a control; and the third one was fed with chromium and germanium citrates, calculated as 50 μg per 1 kg of b.w. for Cr, and germanium citrate – 15 μg Ge per 1 kg of b.w., which results in the activation of the physiological reac- tivity system. However, the integrated physiological effect of citrate compounds of the three elements – chromium, selenium, and germanium – on the rats of the second group was more signifi cant, as confi rmed by a high probability of differences compared against the control group. The complex of microelements, used in these quantities, dem- onstrates signifi cant antioxidant activity, which is typical for each microelement. Conclusions. Feeding mature, 4–8-month-old, rats (feeding started at 4 months and lasted till the end of the 8 th month) and young, 0–4-month-old, rats (feeding started at birth and lasted for 4 months) with “nanoaquacitrates” of germanium, chromium, and sele- nium causes an increase of physiological reactivity in their bodies, which is evidenced as follows, 1) the increase in the ceruloplasmin content in the blood of mothers and infant rats, in haptoglobin and sialic acids – only in mothers, and in protein-bound hexoses – in infant rats; 2) a reduction in the intensity of lipid peroxidation in the blood of mature females and young rats with lower content of lipid hydroperoxides and TBA-active products in the blood of the animals from experiment groups, which is more signifi cant in females; 3) identical orientation of the integrated physiological effect of chromium, selenium, germanium, and the combination of chromium and germanium on the organism of female and young rats which causes general activation of reactivity of the organism and its antioxidant system that is more vivid for females.

The role of antioxidant system dysfunction in the course of gastroesophageal reflux disease in young patients with autoimmune thyroiditis

2021 ◽  
Author(s):  
T. M. Pasiieshvili
Keyword(s):  
Antioxidant Activity ◽  
Gastroesophageal Reflux Disease ◽  
Gastroesophageal Reflux ◽  
Antioxidant Defense ◽  
Reduced Glutathione ◽  
Young Patients ◽  
Antioxidant Defense System ◽  
Total Antioxidant Activity ◽  
Defense System ◽  
Total Antioxidant

Objective — to determine the state of the antioxidant defense system by studying the total antioxidant activity and indicators of the glutathione system in young patients with gastroesophageal reflux disease (GERD) and autoimmune thyroiditis (AIT). Materials and methods. The study involved 120 patients with GERD and AIT (mean age — 21.9 ± 2.7 уears) and 45 subjects with isolated GERD (mean age — 21.2 ± 2.4 уears). All the investigated patients were the students of higher educational institutions. The general antioxidant activity was investigated as the aggregate indicator of the first phase of antioxidant defense and the state of the glutathione link of the antioxidant system (the activity of glutathione reductase, glutathione peroxidase and reduced glutathione). Results. It has been established that in patients with isolated GERD the total antioxidant activity of blood serum was significantly lower in comparison with the control group and was significantly suppressed with the addition of AIT. The decreased synthesis of glutathione reductase, glutathione peroxidase and reduced glutathione was reveled at the comorbid pathologies, as well as dependence of these parameters from the morphological forms of the esophageal mucous lesions: the most intensive alterations were observed  at the erosive form of the disease. Conclusions. The combination of GERD and AIT was accompanied by the changes in the antioxidant defense system parameters. This was manifested in the inhibition of the indicator of the total antioxidant activity and the enzymatic component of the second phase of the system — the glutathione link. These changes were dependent on the morphological form of the esophageal lesions.  

scholarly journals The effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy

2020 ◽  
Vol 22 (100) ◽  
pp. 36-40
Author(s):  
I. S. Varkholiak ◽  
B. V. Gutyj ◽  
V. I. Kushnir ◽  
N. V. Nazaruk ◽  
O. I. Lisnyak ◽  
...  
Keyword(s):  
Heart Failure ◽  
Lipid Peroxidation ◽  
Antioxidant Defense ◽  
Intraperitoneal Administration ◽  
Antioxidant Defense System ◽  
Feed Additives ◽  
Control Group ◽  
Lipid Hydroperoxides ◽  
Defense System ◽  
Experimental Group

Experimental studies obtained in recent years indicate the urgency of developing drugs of complex action to prevent the development of cardiovascular disease in dogs. This requires a much deeper study of the pathogenesis of heart failure, including cardiomyopathy. The aim of the study was the effect of bendamine on the intensity of lipid peroxidation and the activity of the antioxidant defense system of blood in rats in experimental doxorubicin-induced cardiomyopathy. To reproduce heart failure in rats, an experimental model was used by intraperitoneal administration of doxorubicin at a dose of 2.5 mg/kg 3 times a week for two weeks. The study was performed on white sexually mature young male Wistar rats weighing 180–200 g, which were kept on the standard diet of the institute vivarium of the State Research Control Institute of Veterinary Drugs and Feed Additives. For the study, three groups of rats of 6 animals in each were formed: control group – intact animals; experimental group R1, in which animals were simulated doxorubicin-induced cardiomyopathy by intraperitoneal administration of doxorubicin; experimental group R2, in which animals after injection of doxorubicin, intragastrically administered the drug “Bendamine” at a dose of 20 mg/kg. According to studies, doxorubicin intoxication in rats enhances LPO processes, as indicated by an increased content of lipid hydroperoxides by 47.4 %, diene conjugates – by 21.4 % and TBA-active products – by 24.9 % for animals of the control group. Doxorubicin cardiomyopathy in rats has a decrease in the activity of antioxidant enzymes in the blood of animals. In particular, the activity of catalase decreases by 36.9 % (P ≤ 0.001), and the activity of SOD – by 20.3 % (P ≤ 0.001). In the study of the activity of the glutathione link of the antioxidant defense system, similar changes were found as in the study of the activity of catalase and SOD. The use of the drug “Bendamine” in the rats of the experimental group helped to inhibit the excessive formation of lipid peroxidation products and increase the activity of both enzymatic and non-enzymatic parts of the antioxidant system.

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