Molecular Detection of Some Virulence Genes in Salmonella Spp Isolated from Food Samples in Lagos, Nigeria

Virulent and multi drug resistant (MDR) Salmonellaenterica is a foremost cause of foodborne diseases and had serious public health concern globally. The present study was undertaken to identify the pathogenicity and antimicrobial resistance (AMR) profiles of Salmonellaenterica serovars recovered from chicken at wet markets in Dhaka, Bangladesh. A total of 870 cecal contents of broiler, sonali, and native chickens were collected from 29 wet markets. The overall prevalence of S. Typhimurium, S. Enteritidis, and untyped Salmonella spp., were found to be 3.67%, 0.57%, and 1.95% respectively. All isolates were screened by polymerase chain reaction (PCR) for eight virulence genes, namely invA, agfA, IpfA, hilA, sivH, sefA, sopE, and spvC. S. Enteritidis isolates carried all virulence genes whilst S. Typhimurium isolates carried six virulence genes except sefA and spvC. A diverse phenotypic and genotypic AMR pattern was found. Harmonic descending trends of resistance patterns were observed among the broiler, sonali, and native chickens. Interestingly, virulent and MDR Salmonella enterica serovars were found in native chicken, although antimicrobials were not used in their production cycle. The research findings anticipate that virulent and MDR Salmonella enterica are roaming in the wet markets which can easily anchor to the vendor, consumers, and in the food chain.

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Molecular detection of six virulence genes in Pseudomonas aeruginosa isolates detected in children with urinary tract infection

Microbial Pathogenesis ◽

10.1016/j.micpath.2017.03.009 ◽

2017 ◽

Vol 107 ◽

pp. 44-47 ◽

Cited By ~ 10

Author(s):

Ali Badamchi ◽

Hossein Masoumi ◽

Shima Javadinia ◽

Ramin Asgarian ◽

Azardokht Tabatabaee

Keyword(s):

Pseudomonas Aeruginosa ◽

Urinary Tract Infection ◽

Urinary Tract ◽

Tract Infection ◽

Virulence Genes ◽

Molecular Detection

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Occurrence and Antimicrobial Susceptibility Profile of Salmonella Isolates from Animal Origin Food Items in Selected Areas of Arsi Zone, Southeastern Ethiopia, 2018/19

International Journal of Microbiology ◽

10.1155/2021/6633522 ◽

2021 ◽

Vol 2021 ◽

pp. 1-13

Author(s):

Minda Asfaw Geresu ◽

Behailu Assefa Wayuo ◽

Gezahegne Mamo Kassa

Keyword(s):

Antimicrobial Susceptibility ◽

Control Strategies ◽

Raw Milk ◽

Food Samples ◽

Animal Origin ◽

Salmonella Spp ◽

Food Items ◽

Antimicrobial Susceptibility Profile ◽

Arsi Zone ◽

Animal Origin Food

The status of Salmonella and its antimicrobial susceptibility profile in animal origin food items from different catering establishments in Ethiopia is scarce. Hence, this study aimed to investigate the occurrence and antimicrobial susceptibility profile of Salmonella isolates from animal origin food items in the selected areas of Arsi Zone. One hundred ninety-two animal origin food samples were collected and processed for Salmonella isolation. Isolates were tested for their susceptibility to 13 antimicrobials using Kirby–Bauer disk diffusion assay. An overall prevalence of 9.4% (18/192) Salmonella spp. isolates were recovered from animal origin food samples collected from different catering establishments. Seven (21.9%) of “Dulet,” 4 (12.5%) of “Kitfo,” 3 (9.4%) of “Kurt,” 2 (6.3%) of raw milk, 1 (3.1%) of egg sandwich and 1 (3.1%) of cream cake samples were positive for Salmonella. Catering establishments, protective clothing, source of contamination, manner of hand washing, and money handling were among the putative risk factors that were significantly associated ( p < 0.05 ) with Salmonella spp. occurrence. Ampicillin, nitrofurans, and sulphonamide resistance were significantly associated ( p < 0.05 ) with Salmonella spp. occurrence in the selected food items. Three (16.7%), 5 (27.8%), 5 (27.8%), and 4 (22.2%) of the isolates were resistant to 3, 4, 5, and 6 antibiotics, respectively, whereas only a sole isolate was resistant to two antibiotics (viz. ampicillin and kanamycin). In conclusion, the general sanitary condition of the catering establishments, utensils used, and personnel hygienic practices were not to the recommended standards in the current study. Besides, detection of multidrug-resistant strains of Salmonella in animal origin food items from different catering establishments suggests the need for detailed epidemiological and molecular characterization of the pathogen so as to establish the sources of acquisition of resistant Salmonella strains. Hence, implementation of Salmonella prevention and control strategies from farm production to consumption of animal origin food items are crucial.

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DETECTION OF VIRULENCE GENES phoP AND phoQ IN Salmonella spp. USING IN SILICO POLYMERASE CHAIN REACTION

RESEARCH FAIR UNISRI ◽

10.33061/rsfu.v4i1.3414 ◽

2020 ◽

Vol 4 (1) ◽

Author(s):

Stalis Norma Ethica ◽

Hayatun Fuad ◽

Nur Hidayah ◽

Sri Sinto Dewi ◽

Aditya Rahman Ernanto ◽

...

Keyword(s):

In Silico ◽

Salmonella Enterica ◽

Virulence Genes ◽

Gene Detection ◽

Chain Reaction ◽

Salmonella Spp ◽

Detection Tool ◽

In Silico Study ◽

Polymerase Chain ◽

In Silico Pcr

Detection of Salmonella bacteria based on their virulence genes is among essential steps in the eradication of clinical infection by bacteria. In this study, two pair of primers, PhoPF-PhoPR: 5’- CCGCGCAGGAAAAACTCAAA-3’ and 5’-ATCTGTTCCAGCATCACCGG -3’ as well as PhoQF-PhoQR: 5’-AGAGATGATGCGCGTACTGG-3’ and 5’- CAGACGCCCCATGAGAACAT-3’, had been successfully designed using Primer3Plus to detect the presence of phoP and phoQ genes in Salmonella spp. Using genomic DNA of 44 genomic data of Salmonella spp. as templates, PhoPF-PhoPR could produce 520-bp amplicon, while PhoQF-PhoQR could result in 598-bp amplicon. Results of in silico PCR showed that both pairs of primers PhoPF-PhoPR and PhoQF-PhoQR could detect only Salmonella enterica species, and no Salmonella bongori species could be detected based on phoP and phoQ sequences. Both pairs of PhoPF-PhoPR and PhoQF-PhoQR primers were also able to detect the virulence genes in most of the studied subspecies of Salmonella enterica available in silico database unless Arizona subspecies. As conclusion, based on this in silico study, phoP and phoQ genes appeared to be biomarkers for Salmonella enterica species. Both pairs of primers designed in this study has potential to be used as detection tool to differentiate species Salmonella enterica from Salmonella bongori, and also to distinguish S.enterica subsp. enterica from subsp. Arizonae.Keywords: Gene detection, bacterial virulence, phoP, phoQ, Salmonella spp.

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Molecular detection of virulence genes in Staphylococcus aureus associated with bovine mastitis

10.3390/mol2net-05-06259 ◽

2019 ◽

Author(s):

Christhian Villanueva Medina

Keyword(s):

Staphylococcus Aureus ◽

Virulence Genes ◽

Molecular Detection ◽

Bovine Mastitis

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An Evaluation of the Pathogenic Potential, and the Antimicrobial Resistance, of Salmonella Strains Isolated from Mussels

Microorganisms ◽

10.3390/microorganisms10010126 ◽

2022 ◽

Vol 10 (1) ◽

pp. 126

Author(s):

Antonio Lozano-León ◽

Carlos García-Omil ◽

Rafael R. Rodríguez-Souto ◽

Alexandre Lamas ◽

Alejandro Garrido-Maestu

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Virulence Genes ◽

Health Issues ◽

Salmonella Spp ◽

Pathogenic Potential ◽

Antimicrobial Resistance Genes ◽

Important Health ◽

Main Production ◽

Production Areas

Salmonella spp. and antimicrobial resistant microorganisms are two of the most important health issues worldwide. In the present study, strains naturally isolated from mussels harvested in Galicia (one of the main production areas in the world), were genetically characterized attending to the presence of virulence and antimicrobial resistance genes. Additionally, the antimicrobial profile was also determined phenotypically. Strains presenting several virulence genes were isolated but lacked all the antimicrobial resistance genes analyzed. The fact that some of these strains presented multidrug resistance, highlighted the possibility of bearing different genes than those analyzed, or resistance based on completely different mechanisms. The current study highlights the importance of constant surveillance in order to improve the safety of foods.

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QUANTITATIVE ACCOUNT AND DIFFERENTIAL PROPERTIES OF PATHOGENIC BACTERIA ALLOCATED FROM FOOD RAW MATERIALS

Problems of Veterinary Sanitation, Hygiene and Ecology ◽

10.36871/vet.san.hyg.ecol.202002017 ◽

2020 ◽

Vol 1 (2) ◽

pp. 228-235

Author(s):

E.M. Lenchenko ◽

◽

D.V. Stepanov ◽

Keyword(s):

Pathogenic Bacteria ◽

Raw Materials ◽

Food Samples ◽

Labor Costs ◽

Salmonella Spp ◽

Anaerobic Microorganisms ◽

Microbial Cultures ◽

Colony Counting ◽

Quantitative Indicators ◽

Differential Properties

The results of microbiological studies of generally accepted research methods and analysis studies for accelerated calculation the amount of mesophilic aerobic and facultative anaerobic microorganisms isolated from food raw materials are presented. Optimization of sample preparation for research and elimination of routine stages of colony counting significantly increases the number of performed analyzes, saves research time and material costs. Due to the simplicity of operations and a minimum of manual labor,productivity and security are increased, labor costs of staff time are reduced, subjective factors are excluded. The advantages are also in quantitative indicators of the total number of yeast and molds in the studied samples within 72 hours, whereas in the generally accepted method, the indicators are presented only after 5 days. A quantitative analysis of microorganisms of food samples (n = 82) revealed a mismatch of microbiological safety indicators, excess QMAFAnM: 23 (28,0%) samples food raw materials, of which 11 beef samples (47,2%); meat of offal and semi-finished poultry – 12 (20,3%). Based on a comparative assessment of growth-supporting and selective properties, effective diagnostic environments and test systems for differentiating similar types of microorganisms have been tested and selected. From the number of isolates allocated from food raw materials (n = 122), 36 microbial cultures were identified: Salmonella spp. – 10 (13,1%) microbial cultures; coliforms – 25 (24,3%); Listeria monocytogenes – 1 (1,3%).

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Molecular Detection and Antibiotyping of Multidrug-Resistant Salmonella Isolated from Houseflies in a Fish Market

Pathogens ◽

10.3390/pathogens8040191 ◽

2019 ◽

Vol 8 (4) ◽

pp. 191 ◽

Cited By ~ 5

Author(s):

Sobur ◽

Hasan ◽

Haque ◽

Mridul ◽

Noreddin ◽

...

Keyword(s):

Molecular Detection ◽

Multidrug Resistant ◽

Diffusion Method ◽

Resistant Bacteria ◽

Salmonella Spp ◽

Disk Diffusion Method ◽

Antibiotic Resistant ◽

Fish Market ◽

Pcr Method ◽

High Level

Houseflies (Musca domestica) are well-known mechanical vectors for spreading multidrug-resistant bacteria. Fish sold in open markets are exposed to houseflies. The present study investigated the prevalence and antibiotypes of multidrug-resistant (MDR) Salmonella spp. in houseflies captured from a fish market. Direct interviews with fish vendors and consumers were also performed to draw their perceptions about the role of flies in spreading antibiotic-resistant bacteria. A total of 60 houseflies were captured from a local fish market in Bangladesh. The presence of Salmonella spp. was confirmed using PCR method. Antibiogram was determined by the disk diffusion method, followed by the detection of tetA, tetB, and qnrA resistance genes by PCR. From the interview, it was found that most of the consumers and vendors were not aware of antibiotic resistance, but reported that flies can carry pathogens. Salmonella spp. were identified from the surface of 34 (56.7%) houseflies, of which 31 (91.2%) were found to be MDR. This study revealed 25 antibiotypes among the isolated Salmonella spp. All tested isolates were found to be resistant to tetracycline. tetA and tetB were detected in 100% and 47.1% of the isolates, respectively. Among the 10 isolates phenotypically found resistant to ciprofloxacin, six (60%) were found to be positive for qnrA gene. As far as we know, this is the first study from Bangladesh to report and describe the molecular detection of multidrug-resistant Salmonella spp. in houseflies in a fish market facility. The occurrence of a high level of MDR Salmonella in houseflies in the fish market is of great public health concerns.

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