The Progress in Research on Silk Fibroin Blend Membranes

Silk fibroin film, which has good biological compatibility, is suitable for use as biomaterials and medical materials. However, silk fibroin film in dry state is too brittle to be used by itself. Blending with other natural or synthetic polymers could improve the poor mechanical properties of silk fibroin film. In this paper, recent researches in blend film of silk fibroin and their application were summarized.

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Performance of a porous composite scaffold containing silk fibroin: Applied research repair on oral jaw epithelial defects

Materials Express ◽

10.1166/mex.2020.1668 ◽

2020 ◽

Vol 10 (4) ◽

pp. 490-502

Author(s):

Huajun Zhu ◽

Chunyu Qian ◽

Wanshu Xiao ◽

Qiang Zhang ◽

Zili Ge

Keyword(s):

Mechanical Properties ◽

Contact Angle ◽

Silk Fibroin ◽

Composite Scaffold ◽

Epithelial Tissue ◽

Porous Composite ◽

Clinical Value ◽

Freeze Dried ◽

Silk Fibroin Film ◽

Oral Epithelial

Application research on repairing oral and maxillofacial epithelial defects with filin-protein porous composite scaffold. The silk fibroin solution was synthesized by hydrothermal synthesis, and the film was prepared by stirring and pouring. Then silk fibroin film and silk fibroin freeze-dried support were prepared by stirring and smooth casting. It was characterized by FTIR, mechanical properties, dissolution detection, contact Angle and SEM. To evaluate the performance of this material in repairing rabbit oral mucosa and rabbit skin epithelial defects. The characterization shows that the material has good contact Angle, mechanical properties, dissolution and biocompatibility. It has good repair function to rabbit oral epithelial tissue and skin epithelial tissue. Silk fibroin has excellent and unique properties. It has good development prospects and great clinical value in tissue regeneration.

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Preparation and Mechanical and Optical Properties of SF/Pyrrolidone Blend Film

Materials Science Forum ◽

10.4028/www.scientific.net/msf.815.327 ◽

2015 ◽

Vol 815 ◽

pp. 327-331

Author(s):

Jiao Jiao Li ◽

Shan Shan Zhang ◽

Xin Yue Zhang ◽

Shen Zhou Lu

Keyword(s):

Mechanical Properties ◽

Tensile Strength ◽

Silk Fibroin ◽

L929 Cell ◽

Light Transmittance ◽

X Ray Diffraction ◽

Elongation At Break ◽

Blend Film ◽

Blend Films ◽

Tensile Tester

To improve the properties of silk fibroin film, the silk fibroin/pyrrolidone blend films were prepared by mixing silk fibroin and pyrrolidone solution at different ratios. The structure and mechanical properties of blend films were characterized. X-ray diffraction was used to investigate structure of the SF/PYR films, and the results indicated that the blend films were composed of the β-sheet form. Tensile strength and elongation at break of blend films were measured using an instron tensile tester. The results showed that tensile strength and elongation at break of blend films were high enough for application. Furthermore, the films’ flexibility was significantly improved. The optical clarity of blend film was tested by Microplate system. It can be seen from the results that optical clarity is not stable, but in some particular ratios we also can get high light transmittance blend films. At last, biocompatibility of blend films (the rate of the blend film is 30%) was accessed with L929 cell. The results showed that all types of blend films were able to support cell adhesion and proliferation. In addition, the cellular morphology of the cells cultured on blend films was better than that on silk fibroin films. In view of the mechanical properties and optical clarity, combined with their ability to support L929 proliferation suggests that this blend films will offer new options in many different biomedical applications.

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Mechanical Properties and Antithrombogenecity of Blend Membrane of Silk Fibroin

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.288-289.445 ◽

2005 ◽

Vol 288-289 ◽

pp. 445-448

Author(s):

Yin Tang ◽

Chuan Bao Cao ◽

Xilan Ma ◽

Jing Hua Li ◽

He Sun Zhu

Keyword(s):

Mechanical Properties ◽

Silk Fibroin ◽

Poly Vinyl Alcohol ◽

Elongation At Break ◽

Blend Membrane ◽

Preparation Conditions ◽

Measurement Limit ◽

Blend Membranes ◽

Detection Instrument

The silk fibroin blend membranes were prepared by adding a small amount of poly (vinyl alcohol) (PVA) and heparin. By controlling the preparation conditions, the tensile strength and elongation at break reached to 8.86 MPa and 231.8%, respectively. Meanwhile the in vitro antithrombogenecity significant increased. The clot times (APTT, TT and PT) all exceeded the measurement limit of clot detection instrument. It is expectable using these natural materials to prepare small caliber vessel.

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Anti-Inflammatory Properties of Injectable Betamethasone-Loaded Tyramine-Modified Gellan Gum/Silk Fibroin Hydrogels

Biomolecules ◽

10.3390/biom10101456 ◽

2020 ◽

Vol 10 (10) ◽

pp. 1456

Author(s):

Isabel Matos Oliveira ◽

Cristiana Gonçalves ◽

Myeong Eun Shin ◽

Sumi Lee ◽

Rui Luis Reis ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Mechanical Properties ◽

Silk Fibroin ◽

Therapeutic Efficacy ◽

Composite Structures ◽

Gelation Time ◽

Polymeric Materials ◽

Gellan Gum ◽

Traditional Use

Rheumatoid arthritis is a rheumatic disease for which a healing treatment does not presently exist. Silk fibroin has been extensively studied for use in drug delivery systems due to its uniqueness, versatility and strong clinical track record in medicine. However, in general, natural polymeric materials are not mechanically stable enough, and have high rates of biodegradation. Thus, synthetic materials such as gellan gum can be used to produce composite structures with biological signals to promote tissue-specific interactions while providing the desired mechanical properties. In this work, we aimed to produce hydrogels of tyramine-modified gellan gum with silk fibroin (Ty–GG/SF) via horseradish peroxidase (HRP), with encapsulated betamethasone, to improve the biocompatibility and mechanical properties, and further increase therapeutic efficacy to treat rheumatoid arthritis (RA). The Ty–GG/SF hydrogels presented a β-sheet secondary structure, with gelation time around 2–5 min, good resistance to enzymatic degradation, a suitable injectability profile, viscoelastic capacity with a significant solid component and a betamethasone-controlled release profile over time. In vitro studies showed that Ty–GG/SF hydrogels did not produce a deleterious effect on cellular metabolic activity, morphology or proliferation. Furthermore, Ty–GG/SF hydrogels with encapsulated betamethasone revealed greater therapeutic efficacy than the drug applied alone. Therefore, this strategy can provide an improvement in therapeutic efficacy when compared to the traditional use of drugs for the treatment of rheumatoid arthritis.

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Is Dialdehyde Chitosan a Good Substance to Modify Physicochemical Properties of Biopolymeric Materials?

International Journal of Molecular Sciences ◽

10.3390/ijms22073391 ◽

2021 ◽

Vol 22 (7) ◽

pp. 3391

Author(s):

Sylwia Grabska-Zielińska ◽

Alina Sionkowska ◽

Ewa Olewnik-Kruszkowska ◽

Katarzyna Reczyńska ◽

Elżbieta Pamuła

Keyword(s):

Mechanical Properties ◽

Tissue Engineering ◽

Physicochemical Properties ◽

Silk Fibroin ◽

Three Dimensional ◽

Microscopy Imaging ◽

Maximum Deformation ◽

One Step ◽

Chitosan Blends ◽

Fourier Transfer Infrared Spectroscopy

The aim of this work was to compare physicochemical properties of three dimensional scaffolds based on silk fibroin, collagen and chitosan blends, cross-linked with dialdehyde starch (DAS) and dialdehyde chitosan (DAC). DAS was commercially available, while DAC was obtained by one-step synthesis. Structure and physicochemical properties of the materials were characterized using Fourier transfer infrared spectroscopy with attenuated total reflectance device (FTIR-ATR), swelling behavior and water content measurements, porosity and density observations, scanning electron microscopy imaging (SEM), mechanical properties evaluation and thermogravimetric analysis. Metabolic activity with AlamarBlue assay and live/dead fluorescence staining were performed to evaluate the cytocompatibility of the obtained materials with MG-63 osteoblast-like cells. The results showed that the properties of the scaffolds based on silk fibroin, collagen and chitosan can be modified by chemical cross-linking with DAS and DAC. It was found that DAS and DAC have different influence on the properties of biopolymeric scaffolds. Materials cross-linked with DAS were characterized by higher swelling ability (~4000% for DAS cross-linked materials; ~2500% for DAC cross-linked materials), they had lower density (Coll/CTS/30SF scaffold cross-linked with DAS: 21.8 ± 2.4 g/cm3; cross-linked with DAC: 14.6 ± 0.7 g/cm3) and lower mechanical properties (maximum deformation for DAC cross-linked scaffolds was about 69%; for DAS cross-linked scaffolds it was in the range of 12.67 ± 1.51% and 19.83 ± 1.30%) in comparison to materials cross-linked with DAC. Additionally, scaffolds cross-linked with DAS exhibited higher biocompatibility than those cross-linked with DAC. However, the obtained results showed that both types of scaffolds can provide the support required in regenerative medicine and tissue engineering. The scaffolds presented in the present work can be potentially used in bone tissue engineering to facilitate healing of small bone defects.

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Mechanical Properties and Bioactivity of Poly(Lactic Acid) Composites Containing Poly(Glycolic Acid) Fiber and Hydroxyapatite Particles

Nanomaterials ◽

10.3390/nano11010249 ◽

2021 ◽

Vol 11 (1) ◽

pp. 249

Author(s):

Han-Seung Ko ◽

Sangwoon Lee ◽

Doyoung Lee ◽

Jae Young Jho

Keyword(s):

Mechanical Properties ◽

Lactic Acid ◽

Flexural Strength ◽

Interfacial Adhesion ◽

Glycolic Acid ◽

Compact Bone ◽

Poly Lactic Acid ◽

Coupling Reactions ◽

The Poor ◽

Human Compact Bone

To enhance the mechanical strength and bioactivity of poly(lactic acid) (PLA) to the level that can be used as a material for spinal implants, poly(glycolic acid) (PGA) fibers and hydroxyapatite (HA) were introduced as fillers to PLA composites. To improve the poor interface between HA and PLA, HA was grafted by PLA to form HA-g-PLA through coupling reactions, and mixed with PLA. The size of the HA particles in the PLA matrix was observed to be reduced from several micrometers to sub-micrometer by grafting PLA onto HA. The tensile and flexural strength of PLA/HA-g-PLA composites were increased compared with those of PLA/HA, apparently due to the better dispersion of HA and stronger interfacial adhesion between the HA and PLA matrix. We also examined the effects of the length and frequency of grafted PLA chains on the tensile strength of the composites. By the addition of unidirectionally aligned PGA fibers, the flexural strength of the composites was greatly improved to a level comparable with human compact bone. In the bioactivity tests, the growth of apatite on the surface was fastest and most uniform in the PLA/PGA fiber/HA-g-PLA composite.

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Self-healing, Highly Elastic and Amphiphilic Silicone-based Polyurethane for Antifouling Coatings

Journal of Materials Chemistry B ◽

10.1039/d0tb02465a ◽

2021 ◽

Author(s):

Xiaobin Lin ◽

Qingyi Xie ◽

Chunfeng Ma ◽

Guangzhao Zhang

Keyword(s):

Mechanical Properties ◽

Silicone Elastomer ◽

Self Healing ◽

Fouling Resistance ◽

The Poor ◽

Antifouling Coatings ◽

Fouling Release

Silicone elastomer coatings have attracted increasing attention owing to its eco-friendly nature, excellent fouling release ability and drag-reducing property. However, the poor mechanical properties and lack of fouling resistance limits...

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Investigation of the biological activity, mechanical properties and wound healing application of a novel scaffold based on lignin–agarose hydrogel and silk fibroin embedded zinc chromite nanoparticles

RSC Advances ◽

10.1039/d1ra01300a ◽

2021 ◽

Vol 11 (29) ◽

pp. 17914-17923

Author(s):

Reza Eivazzadeh-Keihan ◽

Hooman Aghamirza Moghim Aliabadi ◽

Fateme Radinekiyan ◽

Mohammad Sobhani ◽

Farzane khalili ◽

...

Keyword(s):

Mechanical Properties ◽

Wound Healing ◽

Biological Activity ◽

Silk Fibroin ◽

Agarose Hydrogel ◽

Novel Scaffold ◽

Fibroin Solution ◽

Zinc Chromite

Given the important aspects of wound healing approaches, in this work, an innovative biocompatible nanobiocomposite scaffold was designed and prepared based on cross-linked lignin–agarose hydrogel, extracted silk fibroin solution, and zinc chromite (ZnCr2O4) nanoparticles.

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