Effects of the oxidative stress and genetic changes in varicose vein patients

2018 ◽  
Vol 34 (6) ◽  
pp. 406-413 ◽  
Author(s):  
Devrim Saribal ◽  
Eyup Murat Kanber ◽  
Fatma Sinem Hocaoglu-Emre ◽  
Mehmet C Akyolcu
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Patient Group ◽  
Varicose Veins ◽  
Antioxidant Defense System ◽  
Glutathione S Transferase ◽  
Compensatory Response ◽  
Stress Indicator ◽  
Significant Difference

Background Etiology of the varicose veins is still partly known. It has been proposed that varicose veins formation might be a cause of the oxidative stress and/or cause from genetical reasons. Method The levels of antioxidant defense system enzymes, superoxide dismutase, catalase, glutathione peroxidase, glutathione S-transferase, and an oxidative stress indicator, malondialdehyde, were measured in saphenous vein samples of varicose veins patients. Additionally, genetical polymorphism of glutathione S-transferase theta-1 has been studied. Result In this study, measurements revealed significant increase in catalase and malondialdehyde levels in the patient group, whereas superoxide dismutase and glutathione peroxidase and glutathione S-transferase enzyme activity and comparison of the null mutation frequency in the glutathione S-transferase theta-1 gene did not show a statistically significant difference. Conclusion We propose that the increase in catalase and malondialdehyde activities in our patient group may be related to each other. Increase in catalase levels, an antioxidant enzyme might be a compensatory response to the increase in malondialdehyde levels, an oxidative molecule.

scholarly journals Characteristics of lipid peroxidation processes and factors of the antioxidant defense system in chronic atrophic gastritis and gastric cancer

2022 ◽  
Vol 20 (4) ◽  
pp. 63-70
Author(s):  
O. V. Smirnova ◽  
V. V. Tsukanov ◽  
A. A. Sinyakov ◽  
O. L. Moskalenko ◽  
N. G. Elmanova ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gastric Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Defense System ◽  
Glutathione S Transferase

Background. The problem of gastric cancer remains unresolved throughout the world, while chronic atrophic gastritis (CAG) increases the likelihood of its development by 15 times. In the Russian Federation, the incidence of gastric cancer (GC) is among the highest, with it prevailing among males. One of the leading mechanisms in molecular pathology of membranes is lipid peroxidation (LPO). The severity of oxidative membrane damage depends on concomitant diseases, contributing to emergence and progression of pathological processes and development of cancer. Currently, the problem of LPO is unsolved in biological systems.The aim of this study was to investigate the state of LPO and antioxidant defense system in CAG and GC. Materials and methods. The parameters were studied in 45 patients with CAG and 50 patients with GC. The control group included 50 practically healthy volunteers without gastrointestinal complaints, who did not have changes in the gastric mucosa according to the fibroesophagogastroduodenoscopy (FEGDS) findings.Results. In patients with CAG, an increase in malondialdehyde, superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase was found in the blood plasma compared with the control group. In patients with CAG, lipid peroxidation was activated, and the malondialdehyde level increased by 3.5 times relative to normal values. At the same time, the body fought against oxidative stress by increasing the activity of antioxidant enzymes, such as superoxide dismutase, catalase, glutathione S-transferase, and glutathione peroxidase. All patients with GC showed pronounced oxidative stress in the blood plasma in the form of a 45-fold increase in malondialdehyde. The activity of the main antioxidant enzyme superoxide dismutase was reduced in GC. Catalase was activated, which indicated pronounced oxidative stress, significant damage to blood vessels, and massive cell death. Glutathione-related enzymes (glutathione S-transferase and glutathione peroxidase) and the antioxidant protein ceruloplasmin were activated, which also indicated significant oxidative stress and severe intoxication in patients with GC.Conclusion. Depending on the stage and type of cancer, an in-depth study of lipid peroxidation and factors of the antioxidant defense system can be used to correct therapy and prevent cancer and can serve as markers of progression and prognosis in gastric cancer. 

scholarly journals Precompetitional Weight Reduction Modifies Prooxidative-Antioxidative Status in Judokas

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Katarzyna Knapik ◽  
Karolina Sieroń ◽  
Ewa Wojtyna ◽  
Grzegorz Onik ◽  
Ewa Romuk ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Weight Reduction ◽  
Glutathione S Transferase ◽  
Second Stage ◽  
The Third ◽  
Third Stage ◽  
Protein Sulfhydryl ◽  
Total Oxidative Stress

Objective. The main aim of the study was an assessment of the influence of rapid weight loss on oxidative stress parameters in judokas differing in weight reduction value. Materials and Methods. The study included 30 judokas with an age range of 18-30 years (mean age: 22.4±3.40 years). Enzymatic and nonenzymatic antioxidative markers, lipid peroxidation markers, and total oxidative stress were assessed three times: one week before a competition (the first stage), after gaining the desired weight (the second stage), and one week after the competition (the third stage). Results. Between the first and the second stage, the concentration of lipid hydroperoxides (LPH) decreased significantly. The superoxide dismutase (SOD), copper- and zinc-containing superoxide dismutase (Cu,Zn-SOD), ceruloplasmin (CER), malondialdehyde (MDA), LPH, and total oxidative stress (TOS) concentrations were the lowest one week after the competition. Linear regression indicated that the emphases on increased weight reduction increased the activity of glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST), and protein sulfhydryl (PSH) between the first and the second stage of the study. Moderate weight reduction (2-5%) resulted in elevated levels of SOD, Mn-SOD, LPH, MDA, and TOS in comparison to low and high reductions. An opposite relation was observed in PSH. In judokas, the precompetitional weight reduction range was 0.44-6.10% (mean: 2.93%±1.76%) of the initial body weight. Concentrations of superoxide dismutase (SOD; p<.01), manganese-dependent superoxide dismutase (Mn-SOD; p<.001), and ceruloplasmin (CER; p<.05) decreased between the first and the third stage of the study as well between the second and third one. Before competitions, a decrease in lipid hydroperoxide (LPH; p<.01) concentration was observed. A reduction of malondialdehyde (MDA; p<.05), LPH (p<.01), and total oxidative stress (TOS; p<.05) levels between the first and the final stage occurred. The increase in weight reduction was linearly correlated with the rise of glutathione peroxidase (GPx; p<.05), glutathione reductase (GR; p<.05), glutathione S-transferase (GST; p<.05), and protein sulfhydryl (PSH; p<.05) concentrations between the first and the second stage of the study. Moderate weight reduction (2-5%) resulted in elevated levels of SOD (p<.05), Mn-SOD (p<.05), LPH (p<.05), MDA (p<.05), and TOS (p<.05) in comparison to low and high reductions. An opposite relation was observed in PSH (p<.005). Conclusions. The effect of weight reduction in judo athletes on prooxidative-antioxidative system diversity depends on the weight reduction value.

scholarly journals Effects of Three Types of Massage on Serum Levels of Malondialdehyde, Superoxide Dismutase and Glutathione Peroxidase After One Session of Exhaustive Exercise in Female Futsal Players

2021 ◽  
Vol 10 (4) ◽  
pp. 328-339
Author(s):  
Bahare Heydari ◽  
◽  
Mohsen Ghofrani ◽  
Mohammad Ebrahim Bahram ◽  
◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Recovery Period ◽  
Serum Levels ◽  
The Body ◽  
Exhaustive Exercise ◽  
Oxidative Stress Marker ◽  
Control Group ◽  
Significant Difference

Objective: The production of reactive oxygen species in exercise causes oxidative stress which disturbs the balance of oxidants and antioxidants, causing destructive effects on cells. The present study aims to investigate the effect of three types of massage (Swedish, Russian, Thai) on serum levels of Malondialdehyde (MDA), Glutathione Peroxidase (GPX) and Superoxide Dismutase (SOD) following one session of exhaustive exercise. Methods: This quasi-experimental study was conducted on 48 female futsal players aged 17-22 years in Zahedan, Iran who were selected using a purposive sampling method, and randomly divided into four groups of Swedish massage (Long strokes with pressing and tapping using hands), Russian massage (Medium to high pressure), Thai massage (Pressure to certain parts of the body) and Control. The exercise program was based on Bruce protocol. Serum levels of MDA, GPX and SOD were measured by before and immediately after exercise and after massage. Data analysis was performed using repeated measures ANOVA, considering a significance level of P≤0.05. Results: In all three types of massage, there was a significant decrease in serum level of MDA (0.22±0.08), and a significant increase in GPX (1.84±0.46) and SOD (10.02±2.86) levels after exhaustive (P=0.001). No significant difference was observed in the control group. Conclusion: It seems that Russian, Thai, and Swedish types of massage can affect the serum levels of the MDA (as an oxidative stress marker) and the antioxidant enzymes of GPX and SOD during the post-exercise recovery period.

Abstract TP210: The Genetic Polymorphisms in Oxidative Stress Genes, Superoxide Dismutase and Glutathione Peroxidase, Correlated With Ischemic Stroke

Stroke ◽  
2016 ◽  
Vol 47 (suppl_1) ◽  
Author(s):  
Binod Kumar Yadav ◽  
Renu Yadav ◽  
Byoung-Soo Shin
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Clinical Chemistry ◽  
Biochemical Parameter ◽  
Antioxidant Systems ◽  
Stress Genes ◽  
Increased Risk ◽  
Significant Difference ◽  
Pcr Rflp

Background: Reactive oxygen species (ROS) are controlled by endogenous antioxidant systems that include superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase and antioxidant vitamins. Defect in these systems may develop oxidative stress, and different studies have shown the profound effect of oxidative stress in the stroke because of high susceptibility of the brain to ROS-induced damage. Superoxide is dismutated to H 2 O 2 by SOD which is further converted to H 2 O and O 2 by GPx or catalase in the mitochondria and the lysosomes. The present study was aimed to investigate the association of SOD and GPx polymorphisms in the development of stroke. Methods: A total 982 subjects ( 674 patients, 308 controls) were recruited in this study. Genotyping of SOD1 ( rs1041740 ), SOD2 ( rs4880 ), GPx1 ( rs1050450 ) were performed by LightCycler real-time PCR using LightSNiPreagents and FastStartDNAMasterHybProbe while genotyping of GPx4 ( rs713041 ) were done by PCR-RFLP were done by PCR-RFLP. All biochemical parameter were measured in automated clinical chemistry analyzer, at department of laboratory medicine. Results: Our study demonstrated that the CC+CT/TT genotype of rs4880 significantly increased the risk of stroke (AOR=1.57, 95%CI=1.10-2.24) and SAD-stroke (AOR=1.64, 95%CI=1.17-2.41) compared with CC genotype. In GPx polymorphsims, rs1050450 showed no association with stoke, while rs713041 and the combination analysis of rs1050450 with rs713041 demonstrated the significant association for the stroke development. Interestingly, the inter-combined effect of genetic polymorphism of SOD/GPx showed the significant association for the stroke. Most of the genotypes of these SNPs demonstrated significant difference between control and stroke cases on different biochemical parameters. Conclusion: This study suggests a possible role for oxidative stress in the risk of stroke and clearly states that genetic component of stroke is polygenic as altered SOD and GPx genes interaction showed increased risk for stroke development

scholarly journals Analysis of oxidative stress indicators in Polish patients with prostate cancer

2021 ◽  
Author(s):  
Joanna Maria Drozdz-Afelt ◽  
Beata Barbara Koim-Puchowska ◽  
Piotr Kaminski
Keyword(s):  
Prostate Cancer ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Detoxification Enzymes ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Significant Difference ◽  
Oxidative Stress Indicators

AbstractThe aim of the study was to analyze the activity of antioxidant enzymes (glutathione S-transferase, catalase, superoxide dismutase) and the concentration of malondialdehyde in order to determine the role of detoxification mechanisms in prostate cancer. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) were measured using ready-made kits; lipid peroxidation intensity was determined by the thiobarbituric acid method. Superoxide dismutase was the only enzyme among antioxidant and detoxification enzymes for which a statistically significant difference in activity was found between the studied groups (1.4 U·ml−1 in patients vs. 1.6 U·ml−1 in control). No statistically significant differences were found for GST, CAT or the concentration of MDA between the group of men with prostate cancer and the control group. The lower SOD activity in men with prostate cancer may be due to a deficiency in their antioxidant defense system.

scholarly journals Antioxidants-Related Superoxide Dismutase (SOD), Catalase (CAT), Glutathione Peroxidase (GPX), Glutathione-S-Transferase (GST), and Nitric Oxide Synthase (NOS) Gene Variants Analysis in an Obese Population: A Preliminary Case-Control Study

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 595
Author(s):  
Amani M. T. Gusti ◽  
Safaa Y. Qusti ◽  
Eida M. Alshammari ◽  
Eman A. Toraih ◽  
Manal S. Fawzy
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Superoxide Dismutase ◽  
Nitric Oxide Synthase ◽  
Glutathione Peroxidase ◽  
Disease Risk ◽  
Nucleotide Polymorphisms ◽  
Obesity Risk ◽  
Glutathione S Transferase ◽  
Oxide Synthase

Oxidative stress and antioxidants play an important role in obesity etiopathology. Genetic variants, including single nucleotide polymorphisms (SNPs) of the antioxidant-related genes, may impact disease risk in several populations. This preliminary study aimed to explore the association of 12 SNPs related to superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX), glutathione-S-transferase (GST), and nitric oxide synthase (NOS) genes with obesity susceptibility in a Saudi population. A total of 384 unrelated participants, including 154 (40.1%) obese individuals, were enrolled. TaqMan OpenArray Genotyping assays were used. Six SNPs were significantly more prevalent in obese cohorts: (1) GSTM1 rs1056806*C/T; (2) SOD1 rs2234694*A; (3) SOD2 rs4880*G; (4) SOD3 rs2536512*A; (5) GPX1 rs1800668*A; (6) NOS3 rs1799983*G. Four SNPs were associated with higher obesity risk under heterozygote and dominant models for GSTM1 rs1056806 (C/T), homozygote model for SOD2 rs4880 (A/G), and homozygote and recessive models for GPX1 rs1800668 (A/G). In contrast, SOD3 rs2536512 (A/G) were less likely to be obese under heterozygote and dominant models. The CGAG, CAAA, TGGG, and CGAG combined genotypes showed a higher risk of obesity. In conclusion, the present results suggest that oxidative-stress-related genetic determinants could significantly associate with obesity risk in the study population.

scholarly journals Influence of Classical Massage on Biochemical Markers of Oxidative Stress in Humans: Pilot Study

2021 ◽  
Vol 2021 ◽  
pp. 1-7
Author(s):  
Zofia Skubisz ◽  
Daria Kupczyk ◽  
Aleksander Goch ◽  
Marcin Siedlaczek ◽  
Łukasz Sielski ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Healthy Volunteers ◽  
Biochemical Markers ◽  
Massage Therapy ◽  
Human Organism ◽  
Single Session ◽  
Glutathione S Transferase ◽  
Markers Of Oxidative Stress

Classical massage is one of the most popular forms of conservative treatment in various diseases. Despite the wide scope of research, the mechanisms of massage are not fully known and understood. Apart from the well-described effects on individual body systems, there are few scientific reports on the effects of massage on the human body at the subcellular level. The study was designed to assess changes in oxidative stress parameters in healthy volunteers after a single session of classical massage. 29 healthy volunteers aged 22.24 ± 3.64 participated in the study. Before and 30 minutes after the massage procedures, blood samples were taken by experienced personnel. Biochemical markers of oxidative homeostasis were assessed with highly specific methods for each parameter: oxidase ceruloplasmin, glutathione, malondialdehyde, glutathione peroxidase, glutathione S-transferase, and superoxide dismutase. The study demonstrates that massage therapy caused statistically significant decrease in the concentration of glutathione peroxidase (red blood cells) and increase in the level of glutathione peroxidase (plasma), superoxide dismutase, and malondialdehyde. In contrast, statistically significant changes in the hematocrit, glutathione, NO2-/NO3-, and oxidase ceruloplasmin were not observed. The results show that complex influence of classical massage therapy on human organism may be reflected in parameters of the oxidative stress. To understand this mechanism clearly, further research is needed.

scholarly journals Determination of Malondialdehyde (MDA), Superoxide Dismutase (SOD) and Glutathione Peroxidase (GPx) Levels in Kangal Dogs with Maternal Cannibalism

2017 ◽  
Vol 5 (12) ◽  
pp. 1493
Author(s):  
Nazlı Ercan ◽  
Mustafa Koçkaya
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Blood Serum ◽  
Total Protein ◽  
Active Role ◽  
Antioxidant Defense System ◽  
Control Group ◽  
Mean Values ◽  
The Difference

Oxidative stress, which plays an active role in the pathogenesis of various diseases, continues to be relevant in assessing the condition changes such as aggression. Some of Kangal dogs have been found to eat their own puppies during their first 24 hours following birth, as a case called maternal cannibalism. The present study aims to determine the levels of serum malondialdehyde (MDA), which is a product of lipid peroxidation, and serum glutathione peroxidase (GPx) and superoxide dismutase (SOD) enzymes, which are the parameters of antioxidant defense system, and total protein and albumin levels and to show their relationship with cannibalism. The study material consists of blood and blood serum of 30 Kangal dog breed in total, 15 of which have maternal cannibalism and 15 haven’t observed maternal cannibalism as a control group. Glutathione peroxidase, superoxide dismutase, malondialdehyde levels were investigated by means of spectrophotometric method in blood serum of these animals. The difference between the mean values of total protein, GPx, SOD, MDA and albumin was statistically significant in the results obtained. These findings conclude that oxidative stress and antioxidative metabolism plays a role in the pathogenesis of cannibalism in dogs.

Analysis of Oxidative Stress Indicators in Polish Patients With Prostate Cancer

2021 ◽  
Author(s):  
Joanna Maria Drozdz-Afelt ◽  
Beata Barbara Koim-Puchowska ◽  
Piotr Kaminski
Keyword(s):  
Prostate Cancer ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Thiobarbituric Acid ◽  
Antioxidant Defense System ◽  
Detoxification Enzymes ◽  
Control Group ◽  
Glutathione S Transferase ◽  
Sod Activity ◽  
Significant Difference

Abstract Aims: The aim of the study was to analyze the activity of antioxidant enzymes (glutathione S-transferase, catalase, superoxide dismutase) in order to determine the role of detoxification mechanisms in prostate cancer. The concentration of malondialdehyde, which is an indicator of lipid peroxidation in cancer patients, was also tested.Methods: The activities of superoxide dismutase (SOD), catalase CAT and glutathione S-transferase (GST ) were measured using ready-made kits; lipid peroxidation intensity was determined by the thiobarbituric acid method.Results: Superoxide dismutase was the only enzyme among antioxidant and detoxification enzymes for which a statistically significant difference in activity was found between the studied groups [1.4 U * ml-1 in patients vs. 1.6 U * ml-1 in control]. No statistically significant differences were found for two other biomarkers of antioxidant activity (GST, CAT). There were also no statistically significant differences in the concentration of MDA between the group of men with prostate cancer and the control group.Conclusion: The lower SOD activity in men with prostate cancer may be due to a deficiency in their antioxidant defense system.

scholarly journals Impairment of liver antioxidant defense activity of broiler chickens exposed to benzo[α]pyrene

2011 ◽  
Vol 35 (1) ◽  
pp. 81-87
Author(s):  
I. K. Latif
Keyword(s):  
Oxidative Stress ◽  
Superoxide Dismutase ◽  
Glutathione Peroxidase ◽  
Broiler Chickens ◽  
Antioxidant Defense ◽  
Antioxidant Defense System ◽  
Defense System ◽  
Exogenous Compounds

gainst damage initiated by a variety of endogenous and exogenous compounds. This study was conducted to evaluate the influence of Benzo[α]Pyrene (BαP) administration on the liver antioxidant defense system. Chicks were assigned into five equal groups, as control, tricaprylin group and three groups treated with BαP (1.5 μg, 150 μg or 15 mg/kg BW). Five birds were sacrificed at days 7, 14, 21 and 35 from each group. To assess the liver antioxidant defense system, glutathione (GSH) concentration, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) and catalase (CAT) activities were employed. It was found that 15 mg of BαP level create significant increase (P<0.05) in the GSH level, GSH-Px, SOD and CAT activities (18.483, 9.88, 69.44 and 89.88 respectively) of broilers at day 7 post-instillation (p.i.) in compared with control (12.392, 6.51, 41.08 and 50.83). Nevertheless, significant decrease (P<0.05) in the GSH level, GSH-Px, SOD and CAT activities at 21 and 35 days. A key finding from this study is that exposure to BαP may induces oxidative stress on the liver and impair the antioxidant defense system in broilers.

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