Increased oxidative stress and plasma Hsp70 levels among gasoline filling station attendants

Objectives: Methylcyclopentadienyl manganese tricarbonyl (MMT) is an organic derivative of manganese (Mn) and is used as an antiknock agent and octane enhancer in gasoline. In this article, we tested the oxidative stress and heat stress protein (Hsp) 70 levels of gasoline station attendants to explore potential plasma biomarkers. Furthermore, the dose–response relationship was also identified. Methods: A total of 144 workers, including 96 petrol fillers and 48 cashiers, participated in the study. Ambient concentrations of benzene, toluene, ethylbenzene, and xylene (BTEX) and Mn were monitored at nine filling stations. During the measuring process, the individual cumulative exposure index was calculated. Plasma oxidative stress and Hsp70 levels were also analysed using enzyme-linked immunosorbent assay. Results: The BTEX time-weighted average in office areas was significantly lower than in refuelling areas ( p < 0.05). In refuelling areas, the content of Mn ranged from 6.44 μg/m3 to 127.34 μg/m3, which was much higher than that in office areas (3.16–7.22 μg/m3; p < 0.05). Exposed workers had significantly different plasma oxidative stress indicators compared with the control group, respectively: superoxide dismutase (SOD), 39.18 ± 6.05 U/mL versus 52.84 ± 3.87 U/mL; glutathione peroxidase (GSH-Px), 186.07 ± 15.63 U versus 194.38 ± 10.42 U; and malondialdehyde (MDA), 1.68 ± 0.52 nmol/L versus 1.43 ± 0.64 nmol/L (in all comparisons, p < 0.05). Plasma Hsp70 level in the exposed group (2.77 ± 0.64 ng/mL) was significantly higher than in the control group (2.32 ± 0.87 ng/mL; p < 0.05). Furthermore, Hsp70 levels were inversely correlated with the activities of SOD ( r = −0.305) and GSH-Px ( r = −0.302) in the exposed group ( p < 0.05). Moreover, a positive correlation ( r = 0.653) was found between plasma Hsp70 levels and plasma MDA levels ( p < 0.05). Conclusion: Exposure to MMT-containing gasoline may result in increasing reactive oxygen stress among filling station attendants. Plasma Hsp70 levels could be used as a sensitive responsive biomarker for exposed workers.

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Arsenic Exposure and Methylation Efficiency in Relation to Oxidative Stress in Semiconductor Workers

Atmosphere ◽

10.3390/atmos11050464 ◽

2020 ◽

Vol 11 (5) ◽

pp. 464

Author(s):

Chih-Hong Pan ◽

Ching-Yu Lin ◽

Ching-Huang Lai ◽

Hueiwang Anna Jeng

Keyword(s):

Oxidative Stress ◽

Occupational Exposure ◽

Urinary Excretion ◽

Exposed Group ◽

Control Group ◽

Dimethylarsinic Acid ◽

Staff Members ◽

Hair Samples ◽

Inorganic As ◽

Exposed Workers

This study examined associations between oxidative stress and arsenic (As) exposure and methylation efficiency in semiconductor workers. An As-exposed group (n = 427) and a control group (n = 91) were included. The As-exposure group (n = 427) included 149 maintenance staff members and 278 production staff members representing high As exposure and low As exposure, respectively. The control group included 91 administrative staff members with no or minimal As exposure. An occupational exposure assessment was conducted to assess personal As exposure by measuring As concentrations in urine, hair, and fingernails of the subjects. Urinary As(III), As(V), monomethylarsonic (MMA), and dimethylarsinic acid (DMA) were quantified to assess an internal dose of inorganic As. Urinary 8-hydroxy-2’-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) were measured to asses oxidative DNA damage and lipid peroxidation, respectively. As concentrations in urine, hair, and fingernails significantly increased (p < 0.05) in the As-exposed group in comparison to the control group. Geometric mean urinary concentrations of As, 8-OHdG, and MDA in the As-exposed group significantly exceeded those in the control group. As exposure to As-exposed workers had increased concentrations of 8-OHdG in contrast to those in control subjects. Moreover, urinary 8-OHdG concentrations in the semiconductor workers were positively correlated with urinary total As metabolite (As(III) + As(V) + MMA + DMA) concentrations. Furthermore, urinary excretion of 8-OHdG concentrations in As-exposed workers were positively associated with urinary excretion of MMA concentrations and primary methylation index values (the ration of MMA/inorganic As). However, fingernail and hair samples did not perform as well as urinary samples to measure oxidative stress induced by As exposure. 8-OHdG could serve as a more reliable biomarker for assessing As methylation than MDA did. Occupational exposure to inorganic As was associated with increased oxidative stress among semiconductor workers.

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Decreased paraoxonase 1 activity and increased oxidative stress in low lead−exposed workers

Human & Experimental Toxicology ◽

10.1177/0960327110388536 ◽

2011 ◽

Vol 30 (9) ◽

pp. 1196-1203 ◽

Cited By ~ 13

Author(s):

Thinnakorn Permpongpaiboon ◽

Amar Nagila ◽

Phannee Pidetcha ◽

Kulwadee Tuangmungsakulchai ◽

Soontharee Tantrarongroj ◽

...

Keyword(s):

Oxidative Stress ◽

Lead Exposure ◽

Linear Regression Analysis ◽

Exposed Group ◽

Paraoxonase 1 ◽

Pon1 Activity ◽

Control Group ◽

Oxidative Stress Parameters ◽

Exposed Workers ◽

Stress Parameters

Paraoxonase 1 (PON1) has been proposed as an antioxidant enzyme. Although lead-inhibited PON1 activity has been demonstrated mostly based on in vitro experiments, it is uncertain whether this phenomenon is relevant in pathogenesis of lead-induced oxidative stress in the lead exposure. We examined associations of blood lead levels (BLL) and PON1 activity along with oxidative stress parameters in lead exposure workers. We determined malondialdehyde (MDA), conjugated diene (CD), total peroxides (TP), total antioxidant status (TAS), the oxidative stress index (OSI), and PON1 activity in earthenware factory workers ( n = 60) and control subjects ( n = 65). The lead-exposed group significantly increased lipid peroxidation parameters and OSI compared to the control group ( p < 0.001). The lead-exposed group had significantly decreased PON1 activity and TAS levels compared to the control group ( p < 0.001). Multiple linear regression analysis revealed that BLL were significantly correlated with decreased TAS ( r = −0.496) and PON1 activity ( r = −0.434), but with increased CD ( r = 0.694), TP ( r = 0.614), MDA ( r = 0.788), and OSI ( r = 0.722). Interestingly, BLL at 10 µg/dL significantly decreased PON1 activity and increased oxidative stress parameters with insignificant changes in other biochemical and hematological parameters. Altogether, the reduction of PON1 activity may associate in an imbalance in pro-oxidants and antioxidants, leading to oxidative damage in lead-exposed workers even at low BLL.

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The compromise of dynamic disulfide/thiol homeostasis as a biomarker of oxidative stress in trichloroethylene exposure

Human & Experimental Toxicology ◽

10.1177/0960327115608928 ◽

2016 ◽

Vol 35 (9) ◽

pp. 915-920 ◽

Cited By ~ 11

Author(s):

C Bal ◽

M Büyükşekerci ◽

C Koca ◽

ER Ağış ◽

S Erdoğan ◽

...

Keyword(s):

Oxidative Stress ◽

Trichloroacetic Acid ◽

Exposed Group ◽

Control Group ◽

The Novel ◽

Automated Method ◽

Exposed Workers ◽

Novel Method ◽

Thiol Homeostasis ◽

Healthy Nonsmoker

In this study, we aimed to investigate disulfide/thiol homeostasis in trichloroethylene (TCE) exposure. The study was carried out in 30 nonsmoker TCE-exposed workers with a variety of occupations. Additionally, 30 healthy nonsmoker volunteers were recruited as the control group. TCE exposure was determined by measuring urinary trichloroacetic acid (TCA) concentration. Median urinary TCA levels of exposed workers (20.5 mg/L) were significantly higher than control subjects (5 mg/L). Thiol and disulfide concentrations were determined using a novel automated method. Disulfide/thiol ratio was significantly higher in the exposed group ( p < 0.001). Thiol/disulfide homeostasis was found to be disturbed in TCE-exposed workers. We predict that in TCE-exposed workers this disturbance can be a therapeutic target, and the efficiency of the treatment can easily be monitored by the novel method we used.

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Peripheral levels of superoxide dismutase and glutathione peroxidase in youths in ultra-high risk for psychosis: a pilot study

CNS Spectrums ◽

10.1017/s1092852917000803 ◽

2017 ◽

Vol 24 (03) ◽

pp. 333-337 ◽

Cited By ~ 5

Author(s):

Maiara Zeni-Graiff ◽

Adiel C. Rios ◽

Pawan K. Maurya ◽

Lucas B. Rizzo ◽

Sumit Sethi ◽

...

Keyword(s):

Oxidative Stress ◽

At Risk ◽

Superoxide Dismutase ◽

High Risk ◽

Glutathione Peroxidase ◽

Healthy Control Group ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Ultra High Risk ◽

Healthy Control

IntroductionOxidative stress has been documented in chronic schizophrenia and in the first episode of psychosis, but there are very little data on oxidative stress prior to the disease onset.ObjectiveThis work aimed to compare serum levels of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in young individuals at ultra-high risk (UHR) of developing psychosis with a comparison healthy control group (HC).MethodsThirteen UHR subjects and 29 age- and sex-matched healthy controls (HC) were enrolled in this study. Clinical assessment included the Comprehensive Assessment of At-Risk Mental States (CAARMS), the Semi-Structured Clinical Interview for DSM-IV Axis-I (SCID-I) or the Kiddie-SADS-Present and Lifetime Version (K-SADS-PL), and the Global Assessment of Functioning (GAF) scale. Activities of SOD and GPx were measured in serum by the spectrophotometric method using enzyme-linked immunosorbent assay kits.ResultsAfter adjusting for age and years of education, there was a significant lower activity of SOD and lower GPX activity in the UHR group compared to the healthy control group (rate ratio [RR]=0.330, 95% CI 0.187; 0.584, p<0.001 and RR=0.509, 95% CI 0.323; 0.803, p=0.004, respectively). There were also positive correlations between GAF functioning scores and GPx and SOD activities.ConclusionOur results suggest that oxidative imbalances could be present prior to the onset of full-blown psychosis, including in at-risk stages. Future studies should replicate and expand these results.

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Oxydative stress markers and cytokine levels in rosuvastatin-medicated hypercholesterolemia patients

Turkish Journal of Biochemistry ◽

10.1515/tjb-2018-0267 ◽

2018 ◽

Vol 44 (4) ◽

pp. 530-538

Author(s):

Aysun Çetin ◽

İhsan Çetin ◽

Semih Yılmaz ◽

Ahmet Şen ◽

Göktuğ Savaş ◽

...

Keyword(s):

Oxidative Stress ◽

Interleukin 1 ◽

Paraoxonase 1 ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Phenotypic Effect ◽

Necrosis Factor Alpha ◽

Stress Markers ◽

Tnf Α ◽

Before And After

Abstract Background Limited research is available concerning the relationship between oxidative stress and inflammation parameters, and simultaneously the effects of rosuvastatin on these markers in patients with hypercholesterolemia. We aimed to investigate the connection between cytokines and oxidative stress markers in patients with hypercholesterolemia before and after rosuvastatin treatment. Methods The study consisted of 30 hypercholesterolemic patients diagnosed with routine laboratory tests and 30 healthy participants. The lipid parameters, interleukin-1 beta (IL-1β), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), paraoxonase-1 (PON1) and malondialdehyde (MDA) levels in controls and patients with hypercholesterolemia before and after 12-week treatment with rosuvastatin (10 mg/kg/day), were analyzed by means of enzyme-linked immunosorbent assay. Results It was found that a 12-week cure with rosuvastatin resulted in substantial reductions in IL-1β, IL-6 and TNF-α and MDA levels as in rising activities of PON1 in patients with hypercholesterolemia. Before treatment, the PON1 levels were significantly negatively correlated with TNF-α and IL-6 in control group, while it was positively correlated with TNF-α in patients. Conclusion Our outcomes provide evidence of protected effect of rosuvastatin for inflammation and oxidative damage. It will be of great interest to determine whether the correlation between PON1 and cytokines has any phenotypic effect on PON1.

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Tinnitus in Noise Exposed Workers

10.21203/rs.3.rs-48080/v1 ◽

2020 ◽

Author(s):

Lu Bai ◽

Jianping Yu ◽

Hong Du ◽

Zhuang Shen ◽

Hongyan Yang

Keyword(s):

Occupational Health ◽

Noise Exposure ◽

Past History ◽

Exposed Group ◽

Control Group ◽

Hearing Level ◽

Railway Transportation ◽

Face To Face ◽

Significant Difference ◽

Exposed Workers

Abstract Background Tinnitus in noise exposed workers is worse, which seriously affects the normal life and work. Workers with tinnitus often complain that even going to see the doctor is ineffective. They think tinnitus is caused by occupational noise exposure, which makes tinnitus worse. To find the truth the investigation and analysis of tinnitus including reason, treatment and effect and hearing level was proceeded among all noise-exposed workers in railway transportation mechanical manufacturers in Beijing in 2015. Methods Cross-section study was conducted. 519 noise-exposed workers were selected as exposed group, 515 non noise-exposed workers were selected as control group. Self-designed questionnaire was used in the way of face-to-face to get subjects’ information including social statistical data, occupational history, past history and living habits. Results The incidence of tinnitus was 36.6% among noise-exposed workers, which was significantly higher than that in the control group (χ2 = 40.725, P < 0.05). Meanwhile the incidence of seeing the doctor was 15.8%. The incidence of tinnitus was significantly different in different subgroups of exposed group (χ2 = 6.072, P < 0.05). There was no significant difference between using earphones and tinnitus (P > 0.05). In the exposed group the incidence of hearing abnormal in workers with tinnitus was significantly higher than those without tinnitus (χ2 = 18.570, P < 0.05). Conclusions Right knowledge, attitude and practice (KAP) would be helpful for workers with tinnitus. Occupational health education and supervision of noise-exposed workers should be further strengthened in this industry. Personal protection should be enhanced so as to improve occupational health.

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Astragaloside IV Synergizing with Ferulic Acid Ameliorates Pulmonary Fibrosis by TGF-β1/Smad3 Signaling

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2021/8845798 ◽

2021 ◽

Vol 2021 ◽

pp. 1-9

Author(s):

Jiahuan Tong ◽

Zhisong Wu ◽

Yuchen Wang ◽

Qingxun Hao ◽

Haoge Liu ◽

...

Keyword(s):

Oxidative Stress ◽

Pulmonary Fibrosis ◽

Ferulic Acid ◽

Lung Tissue ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Sham Operation ◽

Astragaloside Iv ◽

Group A ◽

Tgf Β1

Objective. The study aims to research the interventional effect and mechanism of astragaloside IV (Ast) synergizing with ferulic acid (FA) on idiopathic pulmonary fibrosis (IPF) induced by bleomycin in mice. Methods. The mice were randomly divided into seven groups with 10 mice in each group, namely, a sham operation group, a model group, a miRNA-29b (miR-29) group, a miR-29b negative control group (NC group), a FA group, an Ast group, and a combination group. A mouse model of pulmonary fibrosis was established by intratracheal instillation of bleomycin. Samples were collected after 28 days of continuous administration. Hematoxylin and eosin (HE) and Masson staining were used to observe pathological changes in the lung tissue, and the degree of fibrosis was evaluated using the hydroxyproline content. Changes in transforming growth factor-β1 (TGF-β1) and Smad3 in the lung were observed using immunohistochemistry. Enzyme-linked immunosorbent assay (ELISA) was used to detect the level of reactive oxygen species (ROS), malondialdehyde (MDA), and superoxide dismutase (SOD) in the serum. PCR was used to detect the expression of the miR-29b, TGF-β1, Smad3, and nuclear factor E2-related factor 2 (Nrf2) genes. Western blotting was used to detect the content of the TGF-β/Smad3 protein. Results. Ferulic acid combined with astragaloside IV reduced the degree of pulmonary fibrosis and the synthesis of hydroxyproline in lung tissue. The combination of the two also regulated the oxidative stress response ， TGF-β1/Smad3 pathway and miR-29b in lung tissue. Conclusion. Astragaloside IV combined with ferulic acid regulated the oxidative stress of lung tissues and TGF-β1/Smad3 signaling through miR-29b, thereby reducing the degree of pulmonary fibrosis. This provides a reference direction for the clinical treatment of IPF patients.

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Association between H3K36me3 modification and methylation of LINE-1 and MGMT in peripheral blood lymphocytes of PAH-exposed workers

Toxicology Research ◽

10.1093/toxres/tfaa074 ◽

2020 ◽

Vol 9 (5) ◽

pp. 661-668

Author(s):

Xiumei Xing ◽

Zhini He ◽

Ziwei Wang ◽

Ziying Mo ◽

Liping Chen ◽

...

Keyword(s):

Dna Damage ◽

Coke Oven ◽

Peripheral Blood Lymphocytes ◽

Exposed Group ◽

Control Group ◽

Exposure Level ◽

Mgmt Expression ◽

Exposed Workers ◽

Pah Exposure ◽

Genome Level

Abstract To explore the epigenetic alterations in response to DNA damage following polycyclic aromatic hydrocarbons (PAHs) exposure and the crosstalk between different epigenetic regulations, we examined trimethylated Lys 36 of histone H3 (H3K36me3) and methylation of ‘long interspersed element-1 (LINE-1)’ and ‘O 6-methylguanine-DNA methyltransferase (MGMT)’ in peripheral blood lymphocytes (PBLCs) of 173 coke oven workers (PAH-exposed group) and 94 non-exposed workers (control group). The PAH-exposed group showed higher internal PAH exposure level, enhanced DNA damage and increased MGMT expression (all P < 0.001). Notably, the methylation of LINE-1 and MGMT decreased by 3.9 and 40.8%, respectively, while H3K36me3 level was 1.7 times higher in PBLCs of PAH-exposed group compared to control group (all P < 0.001). These three epigenetic marks were significantly associated with DNA damage degree (all P < 0.001) and PAH exposure level in a dose–response manner (all P < 0.001). LINE-1 hypomethylation is correlated with enhanced H3K36me3 modification (β = −0.198, P = 0.002), indicating a synergistic effect between histone modification and DNA methylation at the whole genome level. In addition, MGMT expression was positively correlated with H3K36me3 modification (r = 0.253, P < 0.001), but not negatively correlated with MGMT methylation (r = 0.202, P < 0.05). The in vitro study using human bronchial epithelial cells treated with the organic extract of coke oven emissions confirmed that H3K36me3 is important for MGMT expression following PAH exposure. In summary, our study indicates that histone modification and DNA methylation might have synergistic effects on DNA damage induced by PAH exposure at the whole genome level and H3K36me3 is more essential for MGMT expression during the course.

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Influence of exposure to pesticides on liver enzymes and cholinesterase levels in male agriculture workers

Global NEST Journal ◽

10.30955/gnj.001285 ◽

2014 ◽

Vol 16 (5) ◽

pp. 1006-1015 ◽

Cited By ~ 6

Keyword(s):

Liver Enzymes ◽

Economic Status ◽

Exposed Group ◽

Direct Bilirubin ◽

Control Group ◽

Pesticide Application ◽

Positive Correlation ◽

Agricultural Work ◽

Exposed Workers ◽

Liver Functions

<div> human health and the environment. The study aimed to explore the effects of exposure to pesticides on the liver functions and acetycholinesterase levels in serum (AChES) and red blood cells (AChER) of 100 male participating in agricultural work ranging in age between 20 and 60 year with mean age 37.11±9.3. One hundred males matched for age and socio economic status were recruited as a control group to compare levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), bilriubin, alkaline phosphatase (ALP), total protein, AChES and AChER. The results indicate that AST, ALT and ALP were significantly (P<0.05) increased in pesticide-exposed workers compared with control. There was also a highly significantly (P<0.01) decrease in AChER among male agriculture workers compared with controls. At 10 - 50 times of pesticides applications there was a significantly (P<0.05) decrease in AChER and increase in AST, ALT and ALP activity among exposed group. However, there was a negative correlation between AST, ALT, direct bilirubin, and AChES and age among control group and a positive correlation between ALT and AST and age among exposed group. According to the number of pesticide application, there was a positive correlation between AST, ALP, total and direct bilirubin and number of pesticide application. Agricultural villages in Egypt require more attention to decrease the percentage of literacy among the farmers and raise their health awareness.  </div>

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The Cerebellum under Stress: Dietary African Walnut (Tetracarpidium conophorum)Abrogates Oxidative Stress-driven Neuropathology induced by Chronic Unpredictable Stress

Pan African Journal of Life Sciences ◽

10.36108/pajols/1202/50.0150 ◽

2021 ◽

Vol 5 (1) ◽

Author(s):

Taiwo A. Abayomi

Keyword(s):

Oxidative Stress ◽

Control Diet ◽

Diet Group ◽

Enzyme Linked Immunosorbent Assay ◽

Control Group ◽

Protective Effects ◽

Chronic Unpredictable Stress ◽

Serum Corticosterone ◽

Male Wistar Rats ◽

Group B

Background: Consumption of a healthy diet abundant in antioxidant and anti-inflammatory phytochemicals, offers an effective and least expensive way to prevent neurodegeneration. Herein, the role of Tetracarpidium conophorum (African walnut) enriched diet in chronic stress-induced cerebellar neuropathology was investigated Methodology: Twenty-one male Wistar rats were used for this investigation. Rats were randomly assigned into three groups (A, B, and C), each consisting of 7 rats (n = 7). Group A (Control group) were fed control diet; group B rats were subjected to different chronic unpredictable stressors (CUS) + control diet for 21 days, while group C rats were subjected to CUS + Walnut-enriched diet for 21 days. Serum corticosterone levels, the expression level of antioxidant and inflammatory markers, and cytoarchitectural changes in cerebellum were assessed by enzyme-linked immunosorbent assay (ELISA) immunohistochemistry methods. Results: The walnut-enriched diet prevented astrogliosis, modulated serum corticosterone expression, and tumor necrotic factor-α in the cerebellum. The walnut-enriched diet also caused an improvement in the antioxidant profile, indicating that it suppressed chronic unpredictable stress-induced perturbations. Conclusion: Our results suggest that African walnut exerts protective effects against oxidative stress-driven dysfunction by reducing serum corticosterone levels, modulating oxidative stress pathways, and preventing neuronal morphological damage in the cerebellum.

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