A comparative study of experimental urinary catheters containing silver and zinc for biofilm inhibition

2021 ◽  
Vol 35 (8) ◽  
pp. 1071-1081
Author(s):  
Simonas Vaitkus ◽  
Rafaela Simoes-Torigoe ◽  
Nicholas Wong ◽  
Karcher Morris ◽  
Frederick E Spada ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Biofilm Formation ◽  
Silver Ions ◽  
Oxygen Species ◽  
Urinary Catheters ◽  
Hydrogen Peroxide Production ◽  
Biofilm Inhibition ◽  
Planktonic Growth ◽  
Escherichia Coli Bacteria ◽  
Growth Of Bacteria

Both commercial and experimental antibacterial urinary catheters were investigated for their efficacy in preventing planktonic growth and biofilm formation of Escherichia Coli bacteria in a synthetic urine solution. Experimental antibacterial catheters having thin (<500 µm) dispersions of Ag, Ag/Ag2O, or Zn/Ag2O in polydimethylsiloxane (PDMS) binder all exhibited significant antimicrobial activity, outperforming traditional commercial antibacterial catheters. All experimental catheters prevented planktonic growth of bacteria and did not exhibit biofilm formation during a six-day test period using a colony forming unit (CFU) measurement method. On the other hand, the best performing commercial catheters demonstrated efficacy for only 3 days in planktonic growth tests and formed multiple bacterial colonies in CFU measurements. The Zn/Ag2O/PDMS experimental catheter was the only catheter observed to produce hydrogen peroxide, a reactive oxygen species known to inhibit biofilm formation; lack of detectable hydrogen peroxide production by the Ag2O/PDMS and Ag/Ag2O/PDMS experimental catheters suggests that bactericidal action most likely arises from release of silver ions present in the PDMS coatings.

scholarly journals NADPH Oxidase-Mediated Reactive Oxygen Species Production: Subcellular Localization and Reassessment of Its Role in Plant Defense

2009 ◽  
Vol 22 (7) ◽  
pp. 868-881 ◽  
Author(s):  
Jeannine Lherminier ◽  
Taline Elmayan ◽  
Jérôme Fromentin ◽  
Khadija Tantaoui Elaraqui ◽  
Simona Vesa ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Plasma Membrane ◽  
Nadph Oxidase ◽  
Subcellular Localization ◽  
Acquired Resistance ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Wild Type ◽  
Hydrogen Peroxide Production

Chemiluminescence detection of reactive oxygen species (ROS) triggered in tobacco BY-2 cells by the fungal elicitor cryptogein was previously demonstrated to be abolished in cells transformed with an antisense construct of the plasma membrane NADPH oxidase, NtrbohD. Here, using electron microscopy, it has been confirmed that the first hydrogen peroxide production occurring a few minutes after challenge of tobacco cells with cryptogein is plasma membrane located and NtrbohD mediated. Furthermore, the presence of NtrbohD in detergent-resistant membrane fractions could be associated with the presence of NtrbohD-mediated hydrogen peroxide patches along the plasma membrane. Comparison of the subcellular localization of ROS in wild-type tobacco and in plants transformed with antisense constructs of NtrbohD revealed that this enzyme is also responsible for the hydrogen peroxide production occurring at the plasma membrane after infiltration of tobacco leaves with cryptogein. Finally, the reactivity of wild-type and transformed plants to the elicitor and their resistance against the pathogenic oomycete Phytophthora parasitica were examined. NtrbohD-mediated hydrogen peroxide production does not seem determinant for either hypersensitive response development or the establishment of acquired resistance but it is most likely involved in the signaling pathways associated with the protection of the plant cell.

Controlling biofilm formation by hydrogen peroxide and silver combined disinfectant

2000 ◽  
Vol 42 (1-2) ◽  
pp. 187-192 ◽  
Author(s):  
R. Armon ◽  
N. Laot ◽  
O. Lev ◽  
H. Shuval ◽  
B. Fattal
Keyword(s):  
Hydrogen Peroxide ◽  
Catalase Activity ◽  
Biofilm Formation ◽  
Prolonged Exposure ◽  
Film Growth ◽  
Silver Ions ◽  
Continuous Operation ◽  
Biofilm Growth ◽  
Biofilm Development ◽  
Biofilm Prevention

Controlling biofilm growth in drinking and wastewater pipelines has attracted considerable scientific and technological attention over recent years. In this work, we have examined the biofilm control effectivity of a combined disinfectant comprised of hydrogen peroxide and silver ions. The performance of the combined disinfectant was compared to the effectivity of each of the ingredients alone and to the effectivity of chlorine disinfectant. Biofilm growth was investigated on uncoated and CaCO3 coated galvanized iron samples over prolonged exposure duration. It was found that the CaCO3 film does not significantly affect biofilm development. A combination of hydrogen peroxide and silver ions (30 ppm hydrogen peroxide and 30 ppb silver ions) were as effective in preventing film growth as hydrogen peroxide alone (30 ppm). Both compositions showed significant biofilm prevention effectivity as compared to silver ions alone. Biofilm prevention effectivity of chlorine (approximately 1 ppm) was considerably higher than that of the combined disinfectant. The bacteria that survived after 48 hours disinfection with hydrogen peroxide and the combined disinfectant showed high catalase activity hinting that hydrogen peroxide and the combined disinfectant may have a rather limited effectivity in continuous operation.

scholarly journals Targeting biofilm inhibition using Quercetin – Interaction with bacterial cell membrane and ROS mediated biofilm control

2018 ◽  
Vol 8 (6) ◽  
pp. 292 ◽  
Author(s):  
Sreelatha Sarangapani ◽  
Ayyavoo Jayachitra
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Antimicrobial Activity ◽  
Biofilm Formation ◽  
Biological Activities ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Biofilm Inhibition ◽  
Biofilm Control ◽  
Enhanced Production

Background: Quercetin is an active nutraceutical ingredient widely distributed in foods, vegetables, fruits, and more. Quercetin is a versatile functional food with extensive protective effects against many infectious and degenerative diseases due to their antioxidant activities. Apsergillus niger is a filamentous fungus and the most abundant mold found in the environment. This fungus has been the source of several bioactive compounds and industrial enzymes through biotransformation.Aim: In this report we emphasized the potential of Aspergillus species for the selective conversion of rutin to quercetin, which involved stereoselective and regiospecific reactions with enhanced production and minimization of the formation of toxic wastes. This fungal microbe was able to transform the complex structure of rutin to quercetin with remarkable catalytic activity for the reaction with high product yield. The quercetin produced demonstrated the ability to inhibit biofilm formation and eradicate established biofilm involving the production of reactive oxygen species (ROS) indicative of membrane activity. These results suggest quercetin may have implications in biofilm control targeting reactive oxygen species as a novel therapeutic strategy.Methods: Quercetin was synthesized by microbial biotransformation recruiting Aspergillus niger. The synthesis of quercetin was compared with the chemical process. Furthermore, the quercetin produced by the biotransformation process was characterized by high performance thin layer liquid chromatography. The quercetin produced was assessed for biological activities. The antimicrobial activity, hemolytic activity, inhibition of biofilm by crystal violet staining, and cell viability by confocal laser scanning microscope was assessed. The membrane interaction effect and oxidant scavenging effect by DPPH, Intracellular ROS release, and lipid peroxidation was measured.Results: Quercetin produced by microbial transformation demonstrated antimicrobial activity against S. aureus by effectively inhibiting the growth and dispersion of preformed biofilms. Quercetin demonstrated a significant free radical scavenging activity and significant inhibition of lipid peroxidation. Significant release of reactive oxygen species was observed in bacterial cells.Conclusion: In conclusion, the bio transformed quercetin exhibited disruptive potential of biofilm formation by preventing cell surface attachment and biofilm growth. Therefore, it can be suggested that the major public health benefits could be achieved by substantially increasing the consumption of quercetin rich foods.

Nature of the oxygen species generated by xanthine oxidase involved in secretory histamine release from mast cells

1989 ◽  
Vol 67 (8) ◽  
pp. 397-403 ◽  
Author(s):  
I. Aravind Menon ◽  
Shaila Shirwadkar ◽  
Narendranath S. Ranadive
Keyword(s):  
Hydrogen Peroxide ◽  
Superoxide Dismutase ◽  
Mast Cells ◽  
Xanthine Oxidase ◽  
Cell Lysis ◽  
Oxygen Species ◽  
Hydrogen Peroxide Production ◽  
Oxidase System ◽  
Beneficial Effects ◽  
Cellular Components

The present studies were carried out to characterize the nature of reactive oxygen species generated by the xanthine – xanthine oxidase system involved in the release of histamine by noncytotoxic and cytotoxic mechanisms. To distinguish secretory release from lytic release, mast cells were loaded with 51Cr and the release of 51Cr into the incubation medium was used as a measure of cell lysis. The secretory release of histamine was not inhibited by superoxide dismutase or catalase alone. However, together these agents inhibited the release. This suggests that the combination of superoxide and hydrogen peroxide can evoke secretory release. The lytic release of histamine, as monitored by concomitant release of 51Cr from mast cells at higher concentration of xanthine oxidase or longer periods of incubation, seems to be related to hydrogen peroxide production since catalase inhibited the cell lysis. Since it has been reported that exogenously added hydrogen peroxide at concentrations below 10 mM did not induce cell lysis, the lytic release, although hydrogen peroxide dependent, may not be due to its direct effect on the cell surface. The cell lysis observed in the xanthine – xanthine oxidase system seems to be brought about by a complex mechanism involving the interactions of hydrogen peroxide and superoxide with cellular components. These studies indicate that the beneficial effects of superoxide dismutase seen in biological systems may partly be due to inhibition of the secretory processes stimulated by superoxide.Key words: superoxide, hydrogen peroxide, mast cells, histamine, xanthine oxidase.

Antibiotics Application Strategies to Control Biofilm Formation in Pathogenic Bacteria

2020 ◽  
Vol 21 (4) ◽  
pp. 270-286 ◽  
Author(s):  
Fazlurrahman Khan ◽  
Dung T.N. Pham ◽  
Sandra F. Oloketuyi ◽  
Young-Mog Kim
Keyword(s):  
Biofilm Formation ◽  
Pathogenic Bacteria ◽  
Extracellular Polymeric Substances ◽  
Quorum Quenching ◽  
Resistance Mechanisms ◽  
Bacterial Biofilm ◽  
Bacterial Cells ◽  
High Concentration ◽  
Biofilm Inhibition ◽  
Abiotic Surfaces

Background: The establishment of a biofilm by most pathogenic bacteria has been known as one of the resistance mechanisms against antibiotics. A biofilm is a structural component where the bacterial community adheres to the biotic or abiotic surfaces by the help of Extracellular Polymeric Substances (EPS) produced by bacterial cells. The biofilm matrix possesses the ability to resist several adverse environmental factors, including the effect of antibiotics. Therefore, the resistance of bacterial biofilm-forming cells could be increased up to 1000 times than the planktonic cells, hence requiring a significantly high concentration of antibiotics for treatment. Methods: Up to the present, several methodologies employing antibiotics as an anti-biofilm, antivirulence or quorum quenching agent have been developed for biofilm inhibition and eradication of a pre-formed mature biofilm. Results: Among the anti-biofilm strategies being tested, the sub-minimal inhibitory concentration of several antibiotics either alone or in combination has been shown to inhibit biofilm formation and down-regulate the production of virulence factors. The combinatorial strategies include (1) combination of multiple antibiotics, (2) combination of antibiotics with non-antibiotic agents and (3) loading of antibiotics onto a carrier. Conclusion: The present review paper describes the role of several antibiotics as biofilm inhibitors and also the alternative strategies adopted for applications in eradicating and inhibiting the formation of biofilm by pathogenic bacteria.

Urinary Catheters and Biofilm Formation

2013 ◽  
Vol 3 (3) ◽  
pp. 196-203 ◽  
Author(s):  
Daniele Minardi ◽  
Alessandro Conti ◽  
Matteo Santoni ◽  
Daniele Cantoro ◽  
Oscar Cirioni ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Urinary Catheters

Decomposition of hydrogen peroxide on nickel-silver two-component catalyst

1984 ◽  
Vol 49 (10) ◽  
pp. 2222-2230 ◽  
Author(s):  
Viliam Múčka ◽  
Rostislav Silber
Keyword(s):  
Hydrogen Peroxide ◽  
Chemical Properties ◽  
Silver Ions ◽  
Surface Concentration ◽  
Chloride Ions ◽  
Nickel Silver ◽  
Silver Catalysts ◽  
Physico Chemical ◽  
Low Degree ◽  
Defective Crystal

The catalytic and physico-chemical properties of low-temperature nickel-silver catalysts with nickel oxide concentrations up to 43.8% (m/m) are examined via decomposition of hydrogen peroxide in aqueous solution. The mixed catalysts prepared at 250°C are composed of partly decomposed silver carbonate or oxide and nickel carbonate or hydroxide decomposed to a low degree only and exhibiting a very defective crystal structure. The activity of these catalysts is determined by the surface concentration of silver ions, which is affected by the nickel component present. The latter also contributes to the thermal stability of the catalytic centres of the silver component, viz. the Ag+ ions. The concentration of these ions varies with the temperature of the catalyst treatment, the activity varies qualitatively in the same manner, and the system approaches the Ag-NiO composition. The catalytic centres are very susceptible to poisoning by chloride ions. A previous exposition of the catalyst to a gamma dose of 10 kGy from a 60Co source has no measurable effect on the physico-chemical properties of the system.

scholarly journals Surface Immobilization of Nano-Silver on Polymeric Medical Devices to Prevent Bacterial Biofilm Formation

Pathogens ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 93 ◽  
Author(s):  
Riau ◽  
Aung ◽  
Setiawan ◽  
Yang ◽  
Yam ◽  
...  
Keyword(s):  
Medical Devices ◽  
Biofilm Formation ◽  
Culture Media ◽  
Silver Ions ◽  
Bacterial Biofilm ◽  
Surface Immobilization ◽  
Nano Silver ◽  
Gram Positive Bacteria ◽  
Tissue Microenvironment

: Bacterial biofilm on medical devices is difficult to eradicate. Many have capitalized the anti-infective capability of silver ions (Ag+) by incorporating nano-silver (nAg) in a biodegradable coating, which is then laid on polymeric medical devices. However, such coating can be subjected to premature dissolution, particularly in harsh diseased tissue microenvironment, leading to rapid nAg clearance. It stands to reason that impregnating nAg directly onto the device, at the surface, is a more ideal solution. We tested this concept for a corneal prosthesis by immobilizing nAg and nano-hydroxyapatite (nHAp) on poly(methyl methacrylate), and tested its biocompatibility with human stromal cells and antimicrobial performance against biofilm-forming pathogens, Pseudomonas aeruginosa and Staphylococcus aureus. Three different dual-functionalized substrates—high Ag (referred to as 75:25 HAp:Ag); intermediate Ag (95:5 HAp:Ag); and low Ag (99:1 HAp:Ag) were studied. The 75:25 HAp:Ag was effective in inhibiting biofilm formation, but was cytotoxic. The 95:5 HAp:Ag showed the best selectivity among the three substrates; it prevented biofilm formation of both pathogens and had excellent biocompatibility. The coating was also effective in eliminating non-adherent bacteria in the culture media. However, a 28-day incubation in artificial tear fluid revealed a ~40% reduction in Ag+ release, compared to freshly-coated substrates. The reduction affected the inhibition of S. aureus growth, but not the P. aeruginosa. Our findings suggest that Ag+ released from surface-immobilized nAg diminishes over time and becomes less effective in suppressing biofilm formation of Gram-positive bacteria, such as S. aureus. This advocates the coating, more as a protection against perioperative and early postoperative infections, and less as a long-term preventive solution.

Hydrogen peroxide-responsive AIE probe for imaging-guided organelle targeting and photodynamic cancer cell ablation

2021 ◽  
Author(s):  
Qian Wu ◽  
Youmei Li ◽  
Ying Li ◽  
Dong Wang ◽  
Ben Zhong Tang
Keyword(s):  
Reactive Oxygen Species ◽  
Hydrogen Peroxide ◽  
Cancer Cell ◽  
Reactive Oxygen ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Ablation ◽  
Selective Monitoring ◽  
Organelle Targeting

Hydrogen peroxide (H2O2), as one kind of key reactive oxygen species (ROS), is mainly produced endogenously primarily in the mitochondria. The selective monitoring of H2O2 in living cells is of...

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