Betalain exerts cardioprotective and anti-inflammatory effects against the experimental model of heart failure

2021 ◽  
pp. 096032712110279
Author(s):  
Y Gao ◽  
X Liang ◽  
Z Tian ◽  
Y Ma ◽  
C Sun
Keyword(s):  
Heart Failure ◽  
Experimental Model ◽  
Matrix Protein ◽  
Biological Activities ◽  
Experimental Period ◽  
Matrix Proteins ◽  
Control Group ◽  
Micro Rnas ◽  
Anti Oxidant ◽  
Anti Inflammatory

Betalain is a natural plant pigment known to elicit various biological activities. However, studies on the protective effect of betalain against heart failure have not reported yet. The experimental model of heart failure was created in Wistar rats using isoproterenol (ISO). The animals were randomly assigned into four groups such as sham-control, ISO-induced heart failure, betalain pretreated before ISO induction (50 mg/kg/day), and betalain drug control group were maintained for 6 weeks. At the end of the experimental period, anti-oxidant enzymes, inflammatory markers, matrix proteins, cardiac-specific markers, and micro RNAs were elucidated using RT-PCR, and ELISA analysis. The results demonstrated that the rats induced with ISO displayed an abnormality in cardiac functions, increased oxidative stress markers ( p < 0.01), inflammatory cytokines ( p < 0.01) while abrogated the expression of miR-18a, and increased miR-199a. While betalain pre-treated rats prevented the cardiac failure significantly ( p < 0.01) with improved anti-oxidant enzymes, abrogated the inflammatory signals with restored matrix proteins, cardiac biomarker genes, and attenuated miR-423 and miR-27 compared to heart failure rats. The results of the study suggest that the betalain treatment protected the hearts from failing via microRNA mediated activation the anti-inflammatory signaling and restoring the matrix protein modulation.

Pharmacologically potentials of different substituted coumarin derivatives

2019 ◽  
Author(s):  
Chem Int
Keyword(s):  
Benzene Ring ◽  
Bioactive Compounds ◽  
Biological Activities ◽  
Pharmacological Properties ◽  
Coumarin Derivatives ◽  
Wide Range ◽  
Anti Oxidant ◽  
Anti Inflammatory ◽  
Coumarin Compounds

Coumarin and its derivatives are widely spread in nature. Coumarin goes to agroup as benzopyrones, which consists of a benzene ring connected to a pyronemoiety. Coumarins displayed a broad range of pharmacologically useful profile.Coumarins are considered as a promising group of bioactive compounds thatexhibited a wide range of biological activities like anti-microbial, anti-viral,antiparasitic, anti-helmintic, analgesic, anti-inflammatory, anti-diabetic, anticancer,anti-oxidant, anti-proliferative, anti-convulsant, and antihypertensiveactivities etc. The coumarin compounds have immense interest due to theirdiverse pharmacological properties. In particular, these biological activities makecoumarin compounds more attractive and testing as novel therapeuticcompounds.

Anti-inflammatory and analgesic effects of methanol extract of red cultivar Allium cepa bulbs in rats and mice

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Adeoye Joshua Oyewusi ◽  
Olayinka Ayotunde Oridupa ◽  
Adebowale Bernard Saba ◽  
Ibironke Kofoworola Oyewusi ◽  
Jonny Olufemi Olukunle
Keyword(s):  
Allium Cepa ◽  
Methanol Extract ◽  
Biological Activities ◽  
Hot Water ◽  
Control Group ◽  
Tail Flick ◽  
Control Groups ◽  
Inflammatory Models ◽  
Paw Oedema ◽  
Anti Inflammatory

Abstract Objectives Several cultivars of Allium cepa L. have been studied for anti-inflammatory and analgesic activities but there is inadequate information on such biological activities of the concentrated extracts of the Nigerian grown red cultivar A. cepa bulb. Methods The anti-inflammatory models used in this study were Carrageenan-induced paw oedema and formalin-induced paw lick in rats, while acetic acid-induced abdominal writhing, hot plate reaction, hot water tail flick tests in mice were the analgesic models. Results At 30 min post-induction (pi), the inhibition of paw oedema (62.50%) by 200 mg/kg of methanol extract of red cultivar A. cepa bulb (MERCACB) was significantly (p<0.001) higher than that of indomethacin (15.63%) at 10 mg/kg. The paw oedema inhibition at 60 min pi by MERCACB (76.92%) was significantly higher than that of indomethacin (41.03%). At the early phase of formalin paw-lick test, the pain reaction time (PRT) of rat treated with MERCACB (400 mg/kg) was significantly lower than that of indomethacin and the control groups. The hotplate test revealed that PRT of mice treated with 800 mg/kg of MERCACB were significantly (p<0.01) longer in comparism to indomethacin and control groups. The PRT of mice subjected to thermal pain due to hot water and treated with 800 mg/kg of MERCACB was significantly (p<0.05) longer than that of the control group. Conclusions These findings indicate that MERCACB possesses potent anti-inflammatory and analgesic properties which confirm the traditional use of the plant for the treatment of inflammatory diseases and may be useful as a future therapeutic agent.

Detraction of Diclofenac-Associated Hepatotoxicity by Fresh Beet Root Juice in Male Albino Mice

2021 ◽  
Vol 72 (2) ◽  
pp. 19-26
Author(s):  
Tahani Mohamad Alhazani ◽  
Badr Abdullah Aldahmash ◽  
Doaa Mohamed El-Nagar ◽  
Khalid Elfaki Ibrahim ◽  
Saheed Olaide Anifowose ◽  
...  
Keyword(s):  
Liver Enzymes ◽  
Immunohistochemical Analysis ◽  
Control Group ◽  
Beet Root ◽  
Hepatotoxic Effect ◽  
Anti Oxidant ◽  
Albino Mice ◽  
Anti Inflammatory ◽  
Pre Treatment ◽  
Main Component

The beet root as dietary supplement hepatoprotective ability has gained interest in recent days. The present study was designed to determine the potential hepatoprotective effect of beet root juice as anti-inflammatory and anti-oxidant agent to eliminate the hepatotoxic effect of diclofenac as wide spread analgesic agent. Male albino mice were divided randomly into 4 groups, the 1st group served as control group, the 2nd group received 8 mL/kg of freshly prepared beet root juice, the 3rd group received oral administration 20 mg/kg of diclofenac and the 4th group pre-treated with beet root before one-hour diclofenac administration for 30 days. Biochemical results revealed sharp significant raised levels of liver enzymes level (AST, ALT, ALP and GGT) in the 3rd group that received diclofenac, besides to marked pathological changes manifested by high pathological scoring system such as hepatocytes degeneration, ballooning, infiltration and fibrosis. Immunohistochemical analysis elucidated massive incidence of MDA as an indicator of oxidative stress, moreover great number of neutrophils were seen as main component of inflammation. Whereas, pre-treatment of beet root juice one hour before diclofenac resulted in significant decrease of liver enzymes, clear attenuation of pathological features, decrease of pathological score. A great reduction of MDA in liver tissue and number of neutrophils stained histochemically. It was concluded that beet root juice possessed beneficial hepatoprotective role against diclofenac, as significant anti-oxidant and anti-inflammatory effect.

scholarly journals Effect of Rosmarinic and Caffeic Acids on Inflammatory and Nociception Process in Rats

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Giovana Duzzo Gamaro ◽  
Edna Suyenaga ◽  
Milene Borsoi ◽  
Joice Lermen ◽  
Patrícia Pereira ◽  
...  
Keyword(s):  
Caffeic Acid ◽  
Rosmarinic Acid ◽  
Pleural Cavity ◽  
Biological Activities ◽  
Control Group ◽  
Tail Flick ◽  
Nociceptive Response ◽  
Tail Flick Latency ◽  
Balanced Distribution ◽  
Anti Inflammatory

Rosmarinic acid is commonly found in species of the Boraginaceae and the subfamily Nepetoideae (Lamiaceae). It has a number of interesting biological activities, for example, antiviral, antibacterial, anti-inflammatory, and antioxidant. The aim of the present study was to investigate the effect of the i.p. administration of caffeic and rosmarinic acid (5 and 10 mg/kg) on anti-inflammatory and nociceptive response using carrageenan-induced pleurisy model and tail-flick assay in rats. The analysis of cells in the pleural exudates revealed a reduction of 66% of the number of leukocytes that migrated to the pleural cavity in the animals treated with 5 mg/kg caffeic acid, and of 92.9% for the animals treated with 10 mg/kg in comparison with the control group. These exudates showed a balanced distribution of polymorphonuclear (PMN) and mononuclear (MN) cells, differently from the control group, in which PMN cells were predominant. The analysis to tail-flick latency was increased in the group treated with 10 mg/kg caffeic acid characterizing a nociceptive response. While there was no difference between control group and animals treated with rosmarinic.

scholarly journals DPP-4 inhibitory activity and myocardial salvaging effects of Commiphora mukul in experimental diabetes

2019 ◽  
Vol 8 (3) ◽  
pp. 575
Author(s):  
Manjusha K. Borde ◽  
Ipseeta Ray Mohanty ◽  
Ujwala Maheshwari ◽  
Rajesh Kumar Suman ◽  
Y. A. Deshmukh
Keyword(s):  
Myocardial Infarction ◽  
Experimental Model ◽  
Inhibitory Activity ◽  
Experimental Period ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Commiphora Mukul ◽  
Cardioprotective Effects ◽  
Hs Crp ◽  
Biochemical Indices

Background: Commiphora mukul (Burseraceae) is commonly known as Guggul in Ayurveda. Several studies have reported antidiabetic activity of Commiphora mukul but there are no studies to explore the DPP-4 inhibitory activity and myocardial salvaging effects of Commiphora mukul in setting of diabetes mellitus. The present study was designed to evaluate the cardioprotective efficacy as well as safety of the medicinal plant Commiphora mukul (Guggul) in the experimental model of myocardial infarction co-existing with diabetes.Methods: Diabetes was induced with single dose of streptozotocin (STZ): 45mg/kg ip and myocardial infarction was produced by administering isoproterenol (ISP): (85mg/kg, sc) to rats 24 and 48 h prior to scarification (5th week). After the confirmation of diabetes on 7th day (glucose>200mg/dl), vildagliptin (10 mg/kg) and Commiphora mukul (200 mg/kg) were administered orally from 1st to 5th week (4 weeks). At the end of experimental period, normal control, diabetic-isoproterenol control, vildagliptin and Commiphora mukul group rats were sacrificed for further biochemical investigations as well as histopathological evaluation.Results: Commiphora mukul treatment demonstrated significant antidiabetic as well as myocardial salvaging effects as indicated by restoration of blood glucose, HbA1c and CPK-MB serum DPP-4, hs-CRP levels as compared to diabetic ISP control group. In addition, Commiphora mukul showed significant cardioprotection as indicated by positive correlation between cardiac marker CPK-MB and serum DPP-4. The histopathological assessment of heart, pancreas and biochemical indices of injury confirmed the cardioprotective effects of Commiphora mukul. In addition, Commiphora mukul was found to be safe to the liver and kidney.Conclusions: The natural DPP-4 inhibitor Commiphora mukul demonstrated significant cardioprotective effects in experimental model of myocardial infarction co-existing with diabetes.

scholarly journals Protective Effects of Myrtus communis Linn Fruit and Leaf Extracts on Isoproterenol-Induced Heart Failure in Rat

2021 ◽  
Author(s):  
Nahid Aboutaleb ◽  
Fataneh Hashem-Dabaghian ◽  
Azinmehr Elahian Boroujeni ◽  
Asie Shojaii
Keyword(s):  
Oxidative Stress ◽  
Heart Failure ◽  
Cardiac Fibrosis ◽  
Experimental Period ◽  
Stress Factors ◽  
Control Group ◽  
Alcoholic Extract ◽  
Protective Effects ◽  
Myrtus Communis ◽  
Leaf Extracts

Myrtus communis Linn. (MC) is a cardiotonic plant in traditional Persian medicine. This study was conducted to evaluate the protective effect of MC on isoproterenol-induced heart failure (HF) in rats. Isoproterenol was injected subcutaneously in all groups except the control group for 4 consecutive days to induce myocardial injury in male Wistar rats. In the case of treatment groups, the animals were treated with different doses of the hydro-alcoholic extract of MC fruit or leaves (150, 300, and 700 mg/kg), and were compared with healthy and HF rats. In order to evaluate cardiac function, echocardiography was performed on day 28 after treatment. MC fruit and leaf extracts were administered to all groups except the healthy control group for 28 days by gavage. At the end of the experimental period, the animals were sacrificed and the left ventricle regions of tissue hearts were collected to measure the levels of oxidative stress factors (MDA, SOD, GSH) using ELISA methods. Cardiac fibrosis was evaluated by Mason’s trichrome staining. Hematoxylin-eosin staining was used to assess histopathological changes in cardiac structure.  Our results showed that administration of MC fruit and leaf extracts significantly reduced the MDA level and increased SOD and GSH levels in treated HF rats compared to the HF group (P<0.05). In addition, MC mitigated fibrosis and improved cardiac histological changes compared to the HF group. Collectively, our findings show that MC can be considered as a good candidate to provide cardioprotective effects in HF rats through reduction of oxidative stress and myocardial fibrosis.

scholarly journals Insightful Valorization of the Biological Activities of Pani Heloch Leaves through Experimental and Computer-Aided Mechanisms

Molecules ◽  
2020 ◽  
Vol 25 (21) ◽  
pp. 5153
Author(s):  
Naureen Banu ◽  
Najmul Alam ◽  
Mohammad Nazmul Islam ◽  
Sanjida Islam ◽  
Shahenur Alam Sakib ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Methanol Extract ◽  
Biological Activities ◽  
Biological Effects ◽  
Experimental Models ◽  
Clot Lysis ◽  
Control Group ◽  
Anti Inflammatory

Pani heloch (Antidesma montanum) is traditionally used to treat innumerable diseases and is a source of wild vegetables for the management of different pathological conditions. The present study explored the qualitative phytochemicals; quantitative phenol and flavonoid contents; in vitro antioxidant, anti-inflammatory, and thrombolytic effects; and in vivo antipyretic and analgesic properties of the methanol extract of A. montanum leaves in different experimental models. The extract exhibited secondary metabolites including alkaloids, flavonoids, flavanols, phytosterols, cholesterols, phenols, terpenoids, glycosides, fixed oils, emodines, coumarins, resins, and tannins. Besides, Pani heloch showed strong antioxidant activity (IC50 = 99.00 µg/mL), while a moderate percentage of clot lysis (31.56%) in human blood and significant anti-inflammatory activity (p < 0.001) was achieved with the standard. Moreover, the analgesic and antipyretic properties appeared to trigger a significant response (p < 0.001) relative to in the control group. Besides, an in silico study of carpusin revealed favorable protein-binding affinities. Furthermore, the absorption, distribution, metabolism, excretion, and toxicity analysis and toxicological properties of all isolated compounds adopted Lipinski’s rule of five for drug-like potential and level of toxicity. Our research unveiled that the methanol extract of A. montanum leaves exhibited secondary metabolites that are a good source for managing inflammation, pyrexia, pain, and cellular toxicity. Computational approaches and further studies are required to identify the possible mechanism which responsible for the biological effects.

Synthesis, Characterization and Preliminary Pharmacological Evaluation of Triazolothiadiazoles Derived from some NSAIDs and Thiocarbohydrazide

2018 ◽  
pp. 73-92
Keyword(s):  
Biological Activities ◽  
Enzyme Synthesis ◽  
Control Group ◽  
Paw Edema ◽  
H Nmr ◽  
Pharmacological Test ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Analgesic Activities

The synthesis of new NSAIDs with improved efficacy and selectivity towards COX2, which encouraged by the various biological activities of 1,2,4-triazoles and 1,3,4-thiadiazoles. In this experiment, the production of 1,2,4-triazolothiadiazoles derivatives from Ibuprofen, Naproxen and Indomethacin. We have enhanced anti-inflammatory and analgesic activities by conventional method and microwave-assisted technique, and then compare the time consuming by reaction and yield percent of the product in both way, besides evaluation of anti-inflammatory action of the target compounds by pharmacological test with predictable selectivity towards COX-2 enzyme. Synthesis of the target compounds (P1a-3b, N1a-3b and I1a-3b) has been successfully accomplished by checking purity, characterization, also identification of the synthetic compounds which detected by estimation of physical properties, FT-IR and ¹H-NMR spectroscopy. In vivo potent anti-inflammatory activity of the ending compounds is evaluating in rats utilizing egg-white prompted edema model of inflammation. The experienced compounds (P1a-3b, N1a-3b and I1a-3b) and the reference drugs (Ibuprofen, Naproxen and Indomethacin) produced significant reduction in paw edema in compare to the effect of control group. Wholly tested compounds produced considerable decrease of paw edema in contrast to control group. However, compounds (P3b, N3b and I1b) have considerable more paw edema declining than Ibuprofen, Naproxen and Indomethacin. Intermediate and target compounds are synthesis by microwave method have better result by time and yield in compare with conventional way. The synthesized compounds (Pa1-3b and N1a-3b) may exhibit expected selectivity towards COX-2 enzyme properly due to their large size than its parent Ibuprofen, Naproxen.

scholarly journals Phytochemical profiling, and anti-oxidant, anti-bacterial, and anti-inflammatory properties of Viburnum coriaceum Blume

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Vijayakumar Vijaytha ◽  
R. V. Anupama ◽  
M. Haridas
Keyword(s):  
Methanol Extract ◽  
Biological Activities ◽  
Zone Of Inhibition ◽  
Agar Gel ◽  
Anti Oxidant ◽  
Medicinal Properties ◽  
Phytochemical Profile ◽  
Anti Inflammatory ◽  
Drug Leads ◽  
Diffusion Assay

Abstract Background Viburnums comprise a taxonomic group of plants distributed all over the world and were reported to have many biological activities. Viburnum coriaceum Blume is one of the least explored members of the group. The present study was aimed to explore the phytochemical profile of the plant, Viburnum coriaceum Blume with special emphasis to its anti-oxidant, anti-bacterial, and anti-inflammatory activities. Results Extracts of all parts of plants were found to possess a spectrum of chemicals in considerable amount, including triterpenoids and glycosides. Anti-oxidant property was found in extracts made of any part of the plant. Methanol extract of root expressed a prominent zone of inhibition in agar gel well-diffusion assay involving many microorganisms. Inhibition of enzymes, LOX, and trypsin showed by the hexane extract of root suggested a prominent anti-inflammatory potential of the plant under investigation. Conclusion Phytochemical profiling and the other assays using the plant extracts provide us with a plant having many valuable medicinal properties. Viburnum coriaceum Blume could be noted as a promising material for drug leads.

scholarly journals Biological Activities and Phytochemical Constituents of Trailing Daisy Trilobata: A Review

2019 ◽  
Vol 9 (4-s) ◽  
pp. 888-892
Author(s):  
K Sai Prasanna ◽  
G Jyothi Reddy ◽  
M Kiran ◽  
K Thyaga Raju
Keyword(s):  
Skin Diseases ◽  
Biological Activities ◽  
Pharmacological Activities ◽  
Traditional System ◽  
Wedelia Trilobata ◽  
Anti Oxidant ◽  
Phytochemical Constituents ◽  
Anti Inflammatory ◽  
Anti Fungal ◽  
Natural Medicines

The traditional system of medicinal plants have been found to possess significant anti-inflammatory, antibacterial, anti-fungal, anti-diabetic, analgesic properties etc. Plant-derived drugs are used to cure mental illness, skin diseases, tuberculosis, diabetes, jaundice, hypertension, and cancer. Wedelia Trilobata belongs to family Asteraceae. Leaf, stem, and flower of Wedelia trilobata show anti-microbial, anti-oxidant and anti-inflammatory activity, analgesic activity. Phytochemical screening of the extract has been reported to show the presence of tannins, cardiac glycosides, flavonoids, terpenoids, phenols, saponins, and coumarins. Wedelia Trilobata is also used in reproductive problems, amenorrhea, chest cold, dry cough, and fever. The present review aims to the study was phytoconstituents, biological and pharmacological activities of Wedelia trilobata. This study suggested a possible use of Wedelia trilobata as a source of natural medicines as an anti-inflammatory, anti-oxidant, anti-microbial, hepatoprotective, antidiabetic agents. Keywords: Wedelia Trilobata, Trialing daisy trilobata, Complaya trilobata(L), Sphagneticola Trilobata, pharmacological review.

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