Fabrication and optimization of PCL/PVP nanofibers with Lawsonia inermis for antibacterial wound dressings

2021 ◽  
Vol 29 (9_suppl) ◽  
pp. S1403-S1413
Author(s):  
Mehdi Varsei ◽  
Nadia Rahimi Tanha ◽  
Mohsen Gorji ◽  
Saeedeh Mazinani
Keyword(s):  
Wound Dressing ◽  
Mechanical Performance ◽  
Polymer Concentration ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Gram Negative Bacteria ◽  
Lawsonia Inermis ◽  
Accelerate Wound Healing ◽  
Hydrophobic Polymer ◽  
Surface Method

Anti-infection ability and desirable air permeability (AP) are the important aspects of wound dressings that should be regulated with regard to primary polymers and antibacterial agents. This study aims to fabricate and optimize a wound dressing with antibacterial properties. For this purpose, polycaprolactone (PCL) as a hydrophobic polymer and polyvinylpyrrolidone (PVP) as a hydrophilic polymer were electrospun with Lawsonia inermis (Henna) extract as an antibacterial component based on the experiments proposed by Design-Expert software. The morphology and properties of the wound dressing were studied by scanning electron microscopy, Fourier transform infrared, and AP. The response surface method (RSM) was used to determine the optimal fiber diameter and AP of the samples as a function of the polymer concentration and feed rate. The optimal PCL/PVL/Henna wound dressing has antibacterial properties against Gram-positive and Gram-negative bacteria while being biocompatible according to the MTT assay. These fibrous structures can be used as a wound dressing to prevent infection and accelerate wound healing; thanks to proper Henna release, breathability, swelling ratio, and mechanical performance.

scholarly journals Antibacterial-Integrated Collagen Wound Dressing for Diabetes-Related Foot Ulcers: An Evidence-Based Review of Clinical Studies

Polymers ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2168
Author(s):  
Ibrahim N. Amirrah ◽  
Mohd Farhanulhakim Mohd Razip Wee ◽  
Yasuhiko Tabata ◽  
Ruszymah Bt Hj Idrus ◽  
Abid Nordin ◽  
...  
Keyword(s):  
Wound Healing ◽  
Clinical Trials ◽  
Clinical Studies ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Gentian Violet ◽  
Antibacterial Properties ◽  
Small Sample ◽  
Wound Dressings ◽  
Sample Sizes

Diabetic foot ulcer (DFU) is a chronic wound frequently delayed from severe infection. Wound dressing provides an essential barrier between the ulcer and the external environment. This review aimed to analyse the effectiveness of antibacterial collagen-based dressing for DFU treatment in a clinical setting. An electronic search in four databases, namely, Scopus, PubMed, Ovid MEDLINE(R), and ISI Web of Science, was performed to obtain relevant articles published within the last ten years. The published studies were included if they reported evidence of (1) collagen-based antibacterial dressing or (2) wound healing for diabetic ulcers, and (3) were written in English. Both randomised and non-randomised clinical trials were included. The search for relevant clinical studies (n) identified eight related references discussing the effectiveness of collagen-based antibacterial wound dressings for DFU comprising collagen impregnated with polyhexamethylene biguanide (n = 2), gentamicin (n = 3), combined-cellulose and silver (n = 1), gentian violet/methylene blue mixed (n = 1), and silver (n = 1). The clinical data were limited by small sample sizes and multiple aetiologies of chronic wounds. The evidence was not robust enough for a conclusive statement, although most of the studies reported positive outcomes for the use of collagen dressings loaded with antibacterial properties for DFU wound healing. This study emphasises the importance of having standardised clinical trials, larger sample sizes, and accurate reporting for reliable statistical evidence confirming DFU treatment efficiency.

scholarly journals Degradable tough chitosan dressing for skin wound recovery

2020 ◽  
Vol 9 (1) ◽  
pp. 1576-1585
Author(s):  
Yan Kong ◽  
Xiaoxuan Tang ◽  
Yahong Zhao ◽  
Xiaoli Chen ◽  
Ke Yao ◽  
...  
Keyword(s):  
Water Absorption ◽  
Wound Dressing ◽  
Antibacterial Properties ◽  
High Water ◽  
Skin Wound ◽  
Wound Dressings ◽  
Wound Recovery ◽  
Recovery And Healing ◽  
Optimized Treatment ◽  
Better Than

Abstract The performance of wound dressing determines the effect of wound closure and recovery. Water absorption and bacteriostasis of wound dressings play an important role in wound recovery and healing. In this study, an optimized chitosan wound dressing-tough chitosan dressing (TCS) with high water absorption, high bacteriostasis, and degradability was developed. The chemical structure of chitosan remained stable during the process of optimized treatment, and an increase in mechanical properties was obtained for the dressing. After optimization, the water absorption and antibacterial properties of the chitosan dressing were greatly improved, which is significantly better than sodium alginate dressing. The authors believe that TCS dressing with high hygroscopicity and high bacteriostasis has great potential application value in the field of wound recovery and healing.

Preparation and application of poly (hydroxyl ethyl methacrylate) nanocomposite hydrogels containing iron oxide nanoparticles as wound dressing

2022 ◽  
Vol 30 ◽  
pp. 096739112110631
Author(s):  
Azin Paydayesh ◽  
Leyla Heleil ◽  
Arezoo Sh Dadkhah
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Wound Dressing ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Maximum Increase ◽  
Ethyl Methacrylate ◽  
Nanocomposite Hydrogels ◽  
Degree Of Swelling

In recent years, polymeric hydrogels are widespread in the field of biological materials such as wound dressing and wound care. In this work, we report for the first time the preparation and application of pHEMA nanocomposite hydrogels containing iron oxide nanoparticles as wound dressings. For this purpose, nanocomposite hydrogels based on poly (hydroxyl ethyl methacrylate) (pHEMA) and various amounts of 5, 10, and 15 wt% iron oxide nanoparticles were successfully prepared via radical polymerization. The structure and morphology of nanocomposite hydrogels were determined by Fourier transform infrared spectroscopy (FTIR) and field emission scanning electron microscope (FE-SEM), respectively. The results of gel fraction and the degree of swelling of hydrogels demonstrated that the gel percentage of pHEMA increased, and the degree of swelling decreased with increasing the percentage of nanoparticles. The WVRT and the porosity of hydrogels decreased by increasing the quantity of nanoparticles and were suitable for wound dressing applications. The effect of iron oxide nanoparticles on the mechanical properties of nanocomposite hydrogels was also studied using compression test and hardness shore A durometer. The results indicated that the compression strength, modulus, strain, and hardness are steadily increasing compared to pure hydrogel by adding nanoparticles. The maximum increase was obtained for a hydrogel sample with 15 wt% iron oxide nanoparticles. Antibacterial properties and biocompatibility were determined by the disk-diffusion and MTT assay methods, respectively. Based on the results, nanocomposite hydrogels exhibited higher percentages of cell survival and better antibacterial properties compared to pure pHEMA.

Bi-Layered Disulfiram-Loaded Fiber Membranes With Antibacterial Properties for Wound Dressing

2021 ◽  
Author(s):  
Chenchen Xie ◽  
Jin Yan ◽  
Siyuan Cao ◽  
Ri Liu ◽  
Baishun Sun ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Wound Dressing ◽  
Polyvinylidene Fluoride ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Plasma Modification ◽  
Bottom Surface ◽  
Chemical Compositions ◽  
Fiber Membranes ◽  
Loaded Fiber

Abstract In this study, the bi-layered disulfiram-loaded fiber membranes with antibacterial activity and different surface wettabilities are prepared using electrospinning technology. In the application of wound dressing, the hydrophilic surface of fiber membranes is beneficial for the cell adhesion and drug release to heal the wound, meanwhile the hydrophobic outside surface is able to block water penetration to reduce the probability of wound infection. The obtained bi-layered drug-loaded fiber membranes are composed of polyvinylidene fluoride (PVDF) bottom surface and disulfiram (DSF)/polylactic acid (PLA) top surface. To modify the top surface wettability, the oxygen plasma modification of bi-layered membranes was carried out. We analyzed the morphology, wettability and chemical compositions of bi-layered drug-loaded fiber membranes by various techniques. And the bi-layered disulfiram-loaded membranes showed potent antibacterial activity in vitro against both Escherichia coli (Gram-negative) and Staphylococcus aureus (Gram-positive). Thus, the obtained bi-layered disulfiram-loaded fiber membranes are very suitable for wound dressings application.

scholarly journals Electrospun Antibacterial Nanomaterials for Wound Dressings Applications

Membranes ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 908
Author(s):  
Aysegul Gul ◽  
Izabela Gallus ◽  
Akshat Tegginamath ◽  
Jiri Maryska ◽  
Fatma Yalcinkaya
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Bacterial Infections ◽  
Antimicrobial Agents ◽  
Chronic Wounds ◽  
Antibacterial Properties ◽  
Electrospun Nanofibers ◽  
Electrospinning Process ◽  
Wound Dressings ◽  
Promising Solution

Chronic wounds are caused by bacterial infections and create major healthcare discomforts; to overcome this issue, wound dressings with antibacterial properties are to be utilized. The requirements of antibacterial wound dressings cannot be fulfilled by traditional wound dressing materials. Hence, to improve and accelerate the process of wound healing, an antibacterial wound dressing is to be designed. Electrospun nanofibers offer a promising solution to the management of wound healing, and numerous options are available to load antibacterial compounds onto the nanofiber webs. This review gives us an overview of some recent advances of electrospun antibacterial nanomaterials used in wound dressings. First, we provide a brief overview of the electrospinning process of nanofibers in wound healing and later discuss electrospun fibers that have incorporated various antimicrobial agents to be used in wound dressings. In addition, we highlight the latest research and patents related to electrospun nanofibers in wound dressing. This review also aims to concentrate on the importance of nanofibers for wound dressing applications and discuss functionalized antibacterial nanofibers in wound dressing.

scholarly journals An alginate–PHMB–AgNPs based wound dressing polyamide nanocomposite with improved antibacterial and hemostatic properties

2021 ◽  
Vol 32 (1) ◽  
Author(s):  
Laleh Asadi ◽  
Javad Mokhtari ◽  
Marjan Abbasi
Keyword(s):  
Water Uptake ◽  
Wound Dressing ◽  
Culture Media ◽  
Substrate Surface ◽  
Antibacterial Properties ◽  
Wound Dressings ◽  
Diffusion Method ◽  
Potential Candidate ◽  
Uptake Capacity ◽  
Water Uptake Capacity

AbstractWound dressing should be impenetrable against microorganisms and it should keep the wound wet. Gauze and polyamide (PA) substrate were treated with various concentrations of AgNPs (25, 50, 75, and 100 ppm), PHMB (0.2, 0.4, 0.6, 0.8, and 1% w/v), and constant concentration of alginate (0.5% W/V) using a simple dipping method. Prepared samples were characterized by various techniques including Fourier transform infrared spectroscopy and scanning electron microscopy. The results indicated that the particles were successfully applied onto both substrates with an average diameter of particle size of 78 nm on gauze and 172 nm on the PA substrate surface (based on 50 nanoparticles). Antibacterial activity of the prepared nanocomposite against Staphylococcus aureus (gram-positive) bacteria on PA substrate and gauze were evaluated using the disc diffusion method. The results indicated that the prepared nanocomposites offer favorable antibacterial properties and bacteria would not grow in culture media. The water uptake capacity test of the treated samples was assessed and the data demonstrated that the water absorption rate significantly increases on both treated substrates (gauze and PA substrate) due to the presence of alginate polymer. Also, observing the results of the coagulation test showed that treated samples caused blood clots on the dressing. This is due to the presence of alginate polymer. The present work demonstrates that the prepared samples offer excellent antibacterial properties and good water uptake capacity that capable of being a potential candidate for wound dressings. Due to the results, the produced PA substrate could be an appropriate replacement for the cotton gauze as a wound dressing.

scholarly journals Electrospun Medicated Nanofibers for Wound Healing: Review

Membranes ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 770
Author(s):  
Xinkuan Liu ◽  
Haixia Xu ◽  
Mingxin Zhang ◽  
Deng-Guang Yu
Keyword(s):  
Wound Healing ◽  
Wound Dressing ◽  
Wound Care ◽  
Electrospun Fiber ◽  
Antibacterial Properties ◽  
Biologically Active ◽  
Wound Dressings ◽  
Future Research ◽  
Manufacturing Method ◽  
Active Substances

With the increasing demand for wound care and treatment worldwide, traditional dressings have been unable to meet the needs of the existing market due to their limited antibacterial properties and other defects. Electrospinning technology has attracted more and more researchers’ attention as a simple and versatile manufacturing method. The electrospun nanofiber membrane has a unique structure and biological function similar to the extracellular matrix (ECM), and is considered an advanced wound dressing. They have significant potential in encapsulating and delivering active substances that promote wound healing. This article first discusses the common types of wound dressing, and then summarizes the development of electrospun fiber preparation technology. Finally, the polymers and common biologically active substances used in electrospinning wound dressings are summarized, and portable electrospinning equipment is also discussed. Additionally, future research needs are put forward.

Optimization of Boesenbergia rotunda Extract-Loaded Polyvinyl Alcohol Hydrogel Wound Dressing by Box-Behnken Design

2019 ◽  
Vol 819 ◽  
pp. 38-44
Author(s):  
Pattaranut Eakwaropas ◽  
Yin Yin Myat ◽  
Tanasait Ngawhirunpat ◽  
Theerasak Rojanarata ◽  
Prasopchai Patrojanasophon ◽  
...  
Keyword(s):  
Wound Dressing ◽  
Quadratic Equation ◽  
Wound Dressings ◽  
Elongation At Break ◽  
Box Behnken Design ◽  
Freeze Thaw ◽  
Surface Method ◽  
Thaw Cycle ◽  
Freeze Thaw Cycle ◽  
Boesenbergia Rotunda

The objective of this study was to optimize fabrication variables that affected desirable properties of dressings. Boesenbergia rotunda extract incorporated PVA hydrogels for wound dressings were fabricated by freeze-thaw method. The fabrication variables including PVA concentration (15, 17.5 and 20 % w/w), freeze-thaw cycle (2, 3 and 4 cycles) and extract loading (30, 40 and 50 % w/w) were studied and optimized. Effects of variables on the hydrogel wound dressing properties were determined by using Box-Behnken design and response surface method. Hydrogel properties such as tensile strength, elongation at break, Young’s Modulus, water content, swelling and erosion were measured and used as the designed responses. From statistical data analysis (p <0.05), the polynomial quadratic equation which indicated the significant effects of fabrication variables on the hydrogel properties was generated. In conclusion, desirable B. rotunda extract loaded PVA hydrogel dressing was favorably designed. The optimized PVA concentration, freeze-thaw cycle and extract loading were 17.07 % w/w, 3.86 cycles and 50 % w/w, respectively.

scholarly journals Polymer/Iron-Based Layered Double Hydroxides as Multifunctional Wound Dressings

Pharmaceutics ◽  
2020 ◽  
Vol 12 (11) ◽  
pp. 1130
Author(s):  
Mariana Pires Figueiredo ◽  
Ana Borrego-Sánchez ◽  
Fátima García-Villén ◽  
Dalila Miele ◽  
Silvia Rossi ◽  
...  
Keyword(s):  
Drug Release ◽  
High Performance ◽  
Mechanical Performance ◽  
Release Kinetics ◽  
Wound Dressings ◽  
In Vitro Drug Release ◽  
Improved Performance ◽  
Double Hydroxide ◽  
Double Hydroxides

This work presents the development of multifunctional therapeutic membranes based on a high-performance block copolymer scaffold formed by polyether (PE) and polyamide (PA) units (known as PEBA) and layered double hydroxide (LDH) biomaterials, with the aim to study their uses as wound dressings. Two LDH layer compositions were employed containing Mg2+ or Zn2+, Fe3+ and Al3+ cations, intercalated with chloride anions, abbreviated as Mg-Cl or Zn-Cl, or intercalated with naproxenate (NAP) anions, abbreviated as Mg-NAP or Zn-NAP. Membranes were structurally and physically characterized, and the in vitro drug release kinetics and cytotoxicity assessed. PEBA-loading NaNAP salt particles were also prepared for comparison. Intercalated NAP anions improved LDH–polymer interaction, resulting in membranes with greater mechanical performance compared to the polymer only or to the membranes containing the Cl-LDHs. Drug release (in saline solution) was sustained for at least 8 h for all samples and release kinetics could be modulated: a slower, an intermediate and a faster NAP release were observed from membranes containing Zn-NAP, NaNAP and Mg-NAP particles, respectively. In general, cell viability was higher in the presence of Mg-LDH and the membranes presented improved performance in comparison with the powdered samples. PEBA containing Mg-NAP sample stood out among all membranes in all the evaluated aspects, thus being considered a great candidate for application as multifunctional therapeutic dressings.

scholarly journals Fabrication of Hybrid Nanofibers from Biopolymers and Poly (Vinyl Alcohol)/Poly (ε-Caprolactone) for Wound Dressing Applications

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2104
Author(s):  
Sibusiso Alven ◽  
Blessing Atim Aderibigbe
Keyword(s):  
Mechanical Properties ◽  
Wound Healing ◽  
Vinyl Alcohol ◽  
Wound Dressing ◽  
Chronic Wounds ◽  
Cellular Adhesion ◽  
Wound Dressings ◽  
Poly Vinyl Alcohol ◽  
High Porosity ◽  
Hybrid Nanofibers

The management of chronic wounds is challenging. The factors that impede wound healing include malnutrition, diseases (such as diabetes, cancer), and bacterial infection. Most of the presently utilized wound dressing materials suffer from severe limitations, including poor antibacterial and mechanical properties. Wound dressings formulated from the combination of biopolymers and synthetic polymers (i.e., poly (vinyl alcohol) or poly (ε-caprolactone) display interesting properties, including good biocompatibility, improved biodegradation, good mechanical properties and antimicrobial effects, promote tissue regeneration, etc. Formulation of these wound dressings via electrospinning technique is cost-effective, useful for uniform and continuous nanofibers with controllable pore structure, high porosity, excellent swelling capacity, good gaseous exchange, excellent cellular adhesion, and show a good capability to provide moisture and warmth environment for the accelerated wound healing process. Based on the above-mentioned outstanding properties of nanofibers and the unique properties of hybrid wound dressings prepared from poly (vinyl alcohol) and poly (ε-caprolactone), this review reports the in vitro and in vivo outcomes of the reported hybrid nanofibers.

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