scholarly journals Toxic Effects of Citrus aurantium and C. limon Essential Oils on Spodoptera frugiperda (Lepidoptera: Noctuidae)

2011 ◽  
Vol 6 (9) ◽  
pp. 1934578X1100600 ◽  
Author(s):  
Emilio Villafañe ◽  
Diego Tolosa ◽  
Alicia Bardón ◽  
Adriana Neske
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Spodoptera Frugiperda ◽  
Larval Mortality ◽  
Ethanol Extract ◽  
Toxic Effects ◽  
Citrus Aurantium ◽  
Ethanol Extracts ◽  
Pupal Mortality ◽  
Lepidopteran Pest

Citrus aurantium and C. limon were selected in the search for natural plant insecticides. The essential oils of C. aurantium and C. limon and ethanol extracts of the seeds, pulp, albedo, and peel of C. aurantium were incorporated into the larval diet of the lepidopteran pest Spodoptera frugiperda. Larval and pupal mortality were quantified and adult malformation was observed. C aurantium essential oil had antifeedant action and the mixture of albedo ethanol extract and C aurantium essential oil had toxic effects on S. frugiperda larvae at early stages, when they had not yet produced major damage to the crop. Our results indicated that a mixture of ethanol extract of albedo and C. aurantium essential oil (250 μg of extract mix per g of diet) deterred feeding by 46% and had the highest larval mortality (100%) of the materials tested. The peel extract (250 μg per g of diet) produced an increment in growth rate and diet consumption. However, 40% of the larval and 45% of the pupal populations died after 96 h of treatment. The blend of essential oil and C. aurantium albedo ethanol extract showed the lowest consumption and a poor nutrient conversion into biomass. Finally, the presence of D-limonene and nootkatone in the peel ethanol extract, and C. limon and C. aurantium essential oils, may be the cause of the response in the feeding behavior and toxic effects found on S. frugiperda.

scholarly journals FUMIGATION TOXICITY OF ESSENTIAL OILS AGAINST Rhyzopertha dominica (F.) IN STORED MAIZE GRAIN

2016 ◽  
Vol 29 (2) ◽  
pp. 435-440 ◽  
Author(s):  
VALDEANY NÚBIA DE SOUZA ◽  
CARLOS ROMERO FERREIRA DE OLIVEIRA ◽  
CLÁUDIA HELENA CYSNEIROS MATOS ◽  
DAIANY KEILA FLORENTINO DE ALMEIDA
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Rhyzopertha Dominica ◽  
Fumigant Toxicity ◽  
Citrus Aurantium ◽  
Mentha Spicata ◽  
Insect Mortality ◽  
Maize Grain ◽  
Potential Use ◽  
Fumigation Toxicity

ABSTRACT: The Rhyzopertha dominica F. (Coleoptera: Bostrichidae) is a primary pest of stored grains in many regions of the world. In this work we evaluated the fumigant activity of essential oils of Ocimum basilicum L., Citrus aurantium L., Mentha spicata L. and Croton pulegiodorus Baill on adult R. dominica in stored maize. Tests were conducted to determine lethals concentrations (CL50 and CL100) and mortality (fumigation).The fumigation test was done in containers made of glass containing 10 individuals of R. dominica, where essential oils were applied at different concentrations: O. basilicum and M. spicata (5, 10, 15, 20, 30 and 40µL/L of air), C. aurantium (10, 20, 30, 40, 50 and 60µL/L of air) and C. pulegiodorus (0, 20, 30, 50, 70 and 90µL/L of air). After 48 hours of exposure to the oils the percentage of insect mortality was evaluated. According to LC50 and LC100 the toxicity of essential oils decreased in the following order: O. basilicum> M. spicata> C. pulegiodorus> C. aurantium. The essential oil of O. basilicum exhibited strong fumigant toxicity against R. dominica adults, with a LC50 value of 17.67 µL/L air and LC100 value of 27.15 µL/L air. The C. aurantium essential oil required higher concentrations than O. basilicum, M. spicata and C. pulegiodorus to kill insects. However, all oils evaluated presented fumigating property to promote the control of R. dominica and demonstrated potential use in the management of this coleoptera.

scholarly journals Synergistic effects of binary mixtures of linalool with pyrethroids against fall armyworm

2020 ◽  
Vol 36 ◽  
Author(s):  
Sérgio Macedo Silva ◽  
João Paulo Arantes Rodrigues da Cunha ◽  
César Henrique Souza Zandonadi ◽  
Heli Heros Teodoro de Assunção ◽  
Matheus Gregorio Marques
Keyword(s):  
Essential Oil ◽  
Binary Mixtures ◽  
Spodoptera Frugiperda ◽  
Synergistic Effects ◽  
Larval Mortality ◽  
Lethal Dose ◽  
Fall Armyworm ◽  
Toxicity Tests ◽  
Pyrethroid Insecticides ◽  
Corn Plants

The present work aimed to determine the toxicity of linalool and evaluate the lethal and toxic effects of linalool associated with pyrethroids in binary mixtures to fall armyworm (Spodoptera frugiperda). The insects used in the experiment were obtained from stock breeding initiated from larvae collected from conventional corn plants, grown in an experimental area, in the city of Uberlândia, Minas Gerais. Also, it was obtained essential oil from a variety of Ocimum basilicum, with a high content of linalool (80%), found naturally, as a measure of comparison of different linalool (97.5%) assays. Dose-response bioassays with 3rd instar larvae were performed to determine lethal dose for 50% mortality (LD50) of linalool. Toxicity tests were also performed with O. basilicum essential oil and with pyrethroid insecticides: deltamethrin and its commercial product (Decis 25 EC, Bayer®). After this, combinations between different doses of these products were made and applied on 3rd instar larvae of Spodoptera frugiperda (Smith). Linalool presented high toxicity to S. frugiperda (LD50 = 0.177 μL a.i. μL-1). It was observed neurotoxic effects after the linalool application since the insects presented an aspect of confusion, followed by extreme agitation and finally death. All binary mixtures caused mortality higher than the products applied alone (deltamethrin and linalool) used at 100% LD50, except to 75% LD50 deltamethrin added to 25% LD50 linalool, whose mortality did not differ the products alone, in 24 hours. It was obtained over 90% larval mortality when linalool was combined with 25% LD50 of deltamethrin, in 24 and 48 hours after application, and over 80% of mortality when linalool was combined with 25% LD50 of Decis, only in 48 hours after application. We conclude that linalool is a potential insecticidal and can be associated with pyrethroids to control of S. frugiperda. Further studies are required in order to evaluate the synergistic combinations against field populations of S. frugiperda.

scholarly journals Aktivitas Antioksidan Minyak Atsiri dan Ekstrak Etanol Kulit Batang Sintok (Cinnamomum sintoc Bl.) terhadap 1,1-difenil-2-pikrilhidrazil (DPPH)

2011 ◽  
Vol 1 (1) ◽  
Author(s):  
Sri Adi Sumiwi ◽  
Anas Subarnas ◽  
Supriyatna Supriyatna ◽  
Marline Abdassah Bratadiredja
Keyword(s):  
Antioxidant Activity ◽  
Ascorbic Acid ◽  
Essential Oil ◽  
Essential Oils ◽  
Radical Scavenging ◽  
Ethanolic Extract ◽  
Ethanol Extract ◽  
Scavenging Effect ◽  
Ic50 Value ◽  
Radical Scavenging Effect

Sintoc (Cinnamomum sintoc Bl.) is a plant which is used as medicine. This plant has been known to have an analgesic antiinflamatory activity, therefore it is predicted to have an antioxidant activity. An investigation on antioxidant activity of sintoc essential oils and ethanolic extract of its cortex using ascorbic acid as standard has been carried out. Essential oils and ethanol extract of sintoc cortex was tested using DPPH (1,1-diphenyl-2-pikril-hidrazil) by measuring absorbance using visible spectrophotometer at 518 nm. The methods of this research were distillation of essential oils and extraction of sintoc cortex, determination of the essential oil and extract concentrations required for 50% inhibition of DPPH radical scavenging effect (IC50) with ascorbic acid as the possitive control. The variation concentration  of essential oils are 15, 5, 1, 0.1, 0.5 ppm and 25, 20, 17, 15, 10 ppm for ethanolic extracts. The results showed that the essential oil showed antioxidant activity with IC50 value was 16.29 ppm (5 times lower than ascorbic acid) and then ethanolic extract showed IC50 value 38.89 ppm (11 times lower than ascorbic acid, IC50 of ascorbic acid was 3.35 ppm).

scholarly journals Potent In Vitro Activity of Citrus aurantium Essential Oil and Vitis vinifera Hydrolate Against Gut Yeast Isolates from Irritable Bowel Syndrome Patients—The Right Mix for Potential Therapeutic Use

Nutrients ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1329
Author(s):  
Maura Di Vito ◽  
Maria Grazia Bellardi ◽  
Maurizio Sanguinetti ◽  
Francesca Mondello ◽  
Antonietta Girolamo ◽  
...  
Keyword(s):  
Irritable Bowel Syndrome ◽  
Essential Oil ◽  
Vitis Vinifera ◽  
Essential Oils ◽  
Lactobacillus Species ◽  
Dependent Manner ◽  
Citrus Aurantium ◽  
Candida Spp ◽  
Concentration Dependent Manner ◽  
Irritable Bowel

Background: Irritable bowel syndrome (IBS) is a functional disorder without any pathological alteration, in which the alterations of the Candida/Saccharomyces ratio of the gut microbiota, the balance of pro and anti-inflammatory cytokines and the brain-gut-microbiome axis are important for the development and progression of IBS. The aim of the study was to identify natural products, including essential oils or hydrolates, which were contextually harmless for the gut beneficial strains (e.g., Saccharomyces spp.) but inhibitory for the pathogenic ones (Candida spp.). Methods: The effectiveness of 6 essential oils and 2 hydrolates was evaluated using microbiological tests, carried out on 50 clinical isolates (Candida, Saccharomyces and Galattomyces species) and 9 probiotic strains (Saccharomyces cerevisiae, Lactobacillus species, Akkermansia muciniphila and Faecalibacterium prausnitzii) and immunological and antioxidant assays. Results: The study led to a mixture based on a 1/100 ratio of Citrus aurantium var. amara essential oil / Vitis vinifera cv Italia hydrolate able to contextually reduce, in a concentration-dependent manner, the ability of Candida species to form hyphal filaments and have an interesting immunomodulatory and anti-oxidant action. This mixture can potentially be useful in the IBS treatment promoting the restoration of the intestinal microbial and immunological balance.

scholarly journals Phytochemical Analysis and Antimicrobial, Antinociceptive, and Anti-Inflammatory Activities of Two Chemotypes ofPimenta pseudocaryophyllus(Myrtaceae)

2012 ◽  
Vol 2012 ◽  
pp. 1-15 ◽  
Author(s):  
Joelma Abadia Marciano de Paula ◽  
Maria do Rosário Rodrigues Silva ◽  
Maysa P. Costa ◽  
Danielle Guimarães Almeida Diniz ◽  
Fabyola A. S. Sá ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oils ◽  
Folk Medicine ◽  
Ethanol Extract ◽  
Phytochemical Analysis ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Anti Inflammatory ◽  
Ethanol Extracts

Preparations fromPimenta pseudocaryophyllus(Gomes) L.R. Landrum (Myrtaceae) have been widely used in Brazilian folk medicine. This study aims to evaluate the antimicrobial activity of the crude ethanol extracts, fractions, semipurified substances, and essential oils obtained from leaves of two chemotypes ofP. pseudocaryophyllusand to perform the antinociceptive and anti-inflammatory screening. The ethanol extracts were purified by column chromatography and main compounds were spectrally characterised (1D and 2D1H and13C NMR). The essential oils constituents were identified by GC/MS. The broth microdilution method was used for testing the antimicrobial activity. The abdominal contortions induced by acetic acid and the ear oedema induced by croton oil were used for screening of antinociceptive and anti-inflammatory activities, respectively. The phytochemical analysis resulted in the isolation of pentacyclic triterpenes, flavonoids, and phenol acids. The oleanolic acid showed the best profile of antibacterial activity for Gram-positive bacteria (31.2–125 μg mL−1), followed by the essential oil of the citral chemotype (62.5–250 μg mL−1). Among the semipurified substances,Ppm5, which contained gallic acid, was the most active forCandidaspp. (31.2 μg mL−1) andCryptococcusspp. (3.9–15.6 μg mL−1). The crude ethanol extract and fractions from citral chemotype showed antinociceptive and anti-inflammatory effects.

scholarly journals Larvicidal Activity of Two Cymbopogon Species Leaf Extracts and Essential Oils against Anopheles gambiae Gilles (Diptera: Culicidae)

2019 ◽  
pp. 1-12
Author(s):  
Aurore Christelle Sengue Batti ◽  
Lame Younoussa ◽  
Elias Nchiwan Nukenine ◽  
Mallam Kary Oumarou ◽  
Abraham Fomena
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Anopheles Gambiae ◽  
Plant Extracts ◽  
Mosquito Control ◽  
Larval Mortality ◽  
Chemistry Laboratory ◽  
Mosquito Larvae ◽  
Leaf Extracts ◽  
Methanol Extracts

Aims: The present investigation aimed to assess the toxic effect of hexane, acetone and methanol extracts of Cymbopogon citratus and Cymbopogon giganteus against 3rd and 4th instar of Anopheles gambiae larvae under laboratory conditions. Place and Duration of Study: Plant products were extracted from November 2017 to February 2018 in the Chemistry laboratory, while the larvicidal tests were conducted from April to June 2018 in the laboratory of Applied Zoology of the Faculty of Science, University of Ngaoundere, Cameroon. Methodology: Twenty five (25) 3rd and 4th instars of An. gambiae were subjected to methanol, acetone and methanol plant extracts of the two plants at doses of 1000, 500, 250 and 125 mg/L each while their essential oils were tested at concentrations of 200, 100, 50 and 25 mg/L. Dichlovos tested at the recommended dose of 1000 mg/L was performed as positive control while 1mL of tween-80 in 99 mL of natural breeding site water was used as negative control. Larval mortality was recorded after 24 h post treatment for plant extracts and after 1, 6, 12, 18 and 24 h post-exposure for plant essential oils. Results: At the highest concentration of 1000 mg/L, C. citratus extract caused 100% mortality of mosquito larvae while 84, 81 and 88% mortality of larvae were recorded with hexane, acetone and methanol leaf extract of C. giganteus, respectively. C. citratus (LC50=58.32 mg/L) and C. giganteus (LC50=372.36 mg/L) hexane extracts were more potent than acetone and methanol extracts of the plants. C. citratus extracts were the most effective against larvae compared to C. giganteus extracts. Essential oil of C. citratus (LC50=27.98 mg/L after 1h) was also the most toxic on mosquito larvae compared to C. giganteus (LC50=180.07 mg/L after 1h) essential oil. Conclusion: C. citratus plant and specially its hexane extract and essential oil could be taken into consideration as a new source of botanical insecticide and may be used in the mosquito control programs for An. gambiae larvae elimination in their breeding sites.

scholarly journals Inhibitory effects of different medicinal plants on Candida albicans growth

2018 ◽  
Vol 74 (1) ◽  
pp. 5995-2018 ◽  
Author(s):  
ZORAN TAMBUR ◽  
DESANKA CENIĆ MILOŠEVIĆ ◽  
IVAN MILEUSNIĆ ◽  
RADOJE DODER ◽  
MARJAN MARJANOVIĆ ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Medicinal Plants ◽  
Essential Oil ◽  
Essential Oils ◽  
Dilution Method ◽  
Agar Dilution Method ◽  
Inhibitory Effects ◽  
Strong Activity ◽  
Ethanol Extracts

The objective of this study was to evaluate the in vitro antifungal properties of ethanol extracts and essential oils of medicinal plants from Serbia against Candida albicans (C. albicans) ATCC 10231. Ethanol extracts of fifteen plants were investigated, and their effects were compared with those of three different essential oils. The sensitivity of C. albicans to all plants was tested by the agar dilution method. The assay plates were estimated to contain 300, 150, 75, and 37.5 µg/ml of active extracts and 100, 50, 25, and 12.5 µg/ml of active essential oils. Inocula were applied to agar surfaces, giving approximately 106 cfu/ml of C. albicans. No inhibitory effects were observed for ethanol extracts of Hypericum perforatum and Salvia officinalis (MIC > 300 µg/ml). The most effective were the ethanol extract of Aesculus hippocastanum (MIC = 37.5 µg/ml) and the essential oil of Satureja kitaibelii (MIC = 12.5 µg/ml). Other plants showed MIC from 25 to 300 µg/ml. As far as we know, the inhibitory effects of these medicinal plants against the reference strain of C. albicans have not been commonly investigated in our country. Although the essential oil of Satureja kitaibelii shows strong activity against C. albicans, these results need clinical evaluation..

scholarly journals Toxicity of Chenopodium Ambrosioides L. (Chenopodiaceae) Products From Nigeria Against Three Storage Insects

2010 ◽  
Vol 50 (3) ◽  
pp. 379-384 ◽  
Author(s):  
Abiodun Denloye ◽  
Winifred Makanjuola ◽  
Oluwakemi Teslim ◽  
Oyindamola Alafia ◽  
Adeleke Kasali ◽  
...  
Keyword(s):  
Weight Loss ◽  
Essential Oil ◽  
Callosobruchus Maculatus ◽  
Larval Mortality ◽  
Ethanol Extract ◽  
Fumigant Toxicity ◽  
Chenopodium Ambrosioides ◽  
Storage Insects ◽  
Test Insect ◽  
Coleoptera Tenebrionidae

Toxicity ofChenopodium AmbrosioidesL. (Chenopodiaceae) Products From Nigeria Against Three Storage InsectsTests were carried out to study the toxicity, oviposition suppression, ovicidal and larvicidal effects ofChenopodium ambrosioidesL. as powder, extracts and essential oil againstCallosobruchus maculatusF. (Coleoptera: Bruchidae),Sitophilus zeamaisMotschulsky (Coleoptera: Curculionidae) andTribolium castaneumJacqueline du Val. (Coleoptera: Tenebrionidae). The powder formulation was more toxic toS. zeamaisthan eitherC. maculatusorT. castaneumwith 48 h LC50values of 0.46 g/kg, 1.60 g/kg and 2.14 g/kg, respectively. Ethanol extract was more toxic toC. maculatuswith a 48 h LC50value of 0.023 g/l, than other test insect species. The essential oil treatment demonstrated higher fumigant toxicity againstC. maculatusthanS. zeamaiswith 24 h LC50values of 1.33 μl/l and 1.90 μl/l respectively. The oil vapour showed activity againstC. maculatusegg, but had no appreciable larval mortality. The weight loss of grains admixed withC. ambrosioidespowder was lower than the controls after 150 days of field storage.

scholarly journals Association of peanut cultivars and aqueous neem extract in the feeding and development of Spodoptera frugiperda (J. E. Smith)

2020 ◽  
Vol 13 ◽  
pp. e909
Author(s):  
Arlindo Leal Boiça Junior ◽  
Nara Elisa Lobato Rodrigues ◽  
Julio Cesar Janini ◽  
Daline Benites Bottega ◽  
Vitor Quintela Sousa ◽  
...  
Keyword(s):  
Spodoptera Frugiperda ◽  
Larval Mortality ◽  
Total Mortality ◽  
Preference Test ◽  
Pupal Weight ◽  
Adult Longevity ◽  
Neem Extract ◽  
Peanut Cultivars ◽  
Petri Dishes ◽  
Pupal Mortality

The current study aimed to test how aqueous neem Azadirachta indica A. Juss. extract affects the attractiveness, feeding and development of Spodoptera frugiperda (J. E. Smith) on different: peanut cultivars. Tests were carried out under laboratory conditions with the cultivars IAC 503 and IAC 147 and aqueous neem extract in concentrations (mass/volume) of 5 and 10%, obtained from seeds and dried in an oven at a temperature of 35 to 38 °C for 15 days, with caterpillars offered a food choice preference test. For this, leaf discs from each treatment were placed in Petri dishes into which three first instar caterpillars were released. To assess resistance biology, single newly-hatched caterpillars were transferred to individual Petri dishes, where the following biological parameters were measured: larval mortality after 1, 3, 5, 7 and 10 days; pupal mortality; total mortality; weight of 10-day-old caterpillars; pupal weight at 24 hours; and adult longevity. The main conclusions of the work, include: cultivar IAC 147 was less attractive and consumed less; cultivar IAC 503 plus 10% neem extract reduced leaf consumption; cultivar type did not affect caterpillar biological development, and 10% neem extract caused 100% mortality in S. frugiperda.

scholarly journals REPELLENCY OF CELERY ESSENTIAL OILS (Apium graveolens L.) AGAINST Spodoptera frugiperda (Lepidoptera: Noctuidae) IN THE LABORATORY

2021 ◽  
Vol 21 (2) ◽  
pp. 91-96
Author(s):  
Trisnani Alif ◽  
Fita Fitriatul Wahidah
Keyword(s):  
Essential Oil ◽  
Essential Oils ◽  
Spodoptera Frugiperda ◽  
Concentration Level ◽  
Apium Graveolens ◽  
Herbivorous Insects ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design ◽  
Lepidoptera Noctuidae

Repellency of celery essential oil (Apium graveolens L.) against Spodoptera frugiperda (Lepidoptera: Noctuidae) in thelaboratory. Essential oil is one of the ingredients that has repellent ability to herbivorous insects. Celery is one of plantscontaining essential oil which may also have the repellent ability. This study was aimed to determine the differences in therepellent ability of celery essential oils compounds that were taken from 3 different locations against S. frugiperda in thelaboratory. This research was arranged in a factorial completely randomized design (CRD) with two factors. The first factorwas the origin of the celery plants (Malang, Surabaya, and Lamongan) and the second factor was the concentration level ofessential oils (1000, 2000, and 3000 ppm) which was repeated three times. The reliability test was carried out using filter papermethod. The results showed that the essential oil extracted from celery originated from Malang with 3000 ppm concentrationhad the highest percentage level of repellency (level 5) that reached 93.33% of repellency. Location and concentration factorshad no different effect on S. frugiperda larvae repellency. There was no correlation between location and concentration on therepellency of S. frugiperda larvae.

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