scholarly journals Characterization and Quantitation of Polyphenolic Compounds in Senna gardneri and S. georgica from the Northeast of Brazil

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301
Author(s):  
Irvila Ricarte de O. Maia ◽  
Maria Teresa Salles Trevisan ◽  
Maria Goretti de V. Silva ◽  
Andrea Breuer ◽  
Robert W. Owen
Keyword(s):  
Dry Weight ◽  
Polyphenolic Compounds ◽  
Weight Basis ◽  
Rich Source ◽  
Methanol Extracts ◽  
Phytochemical Composition

Because there is a dearth of data on the taxon Senna, the aim of this study was to determine the phytochemical composition of various botanical parts of Senna gardneri Irwin & Barneby and Senna georgica Irwin & Barneby. A total of 24 polyphenolic compounds were identified in methanol extracts. The higher concentration (on a dry weight basis) of polyphenolic compounds, was detected in the leaves (29.3 g/kg) and roots (17.3 g/kg) of S. gardneri. By contrast, a lower concentration of polyphenolic compounds were detected in the roots of S. georgica (11.8 g/kg), followed by the bark (5.8 g/kg), leaves (0.7 g/kg) and fruits (0.3 g/kg). Flavonoids comprised the major polyphenolic compounds in leaves of S. gardneri (98 %) and S. georgica (100 %) and the bark of S. georgica (86 %). Whereas the composition was quite different in the roots, of which the major components were stilbenes and naphthapyrones at 87 % and 13 % in S. georgica roots and 64 % and 28 % in S. gardneri roots. The fruits of S. georgica contained only one anthraquinone. In conclusion the roots of S. gardneri (11.023 g/kg) and S. georgica (10.285 g/kg) are a rich source of stilbenes, which have promising cancer chemopreventive capacities.

scholarly journals Characterization and Quantitation of Polyphenolic Compounds in Senna macranthera var pudibunda From the Northeast of Brazil

2019 ◽  
Vol 14 (7) ◽  
pp. 1934578X1985170
Author(s):  
Irvila Ricarte de O. Maia ◽  
Maria Teresa Salles Trevisan ◽  
Maria Goretti de V. Silva ◽  
Andrea Breuer ◽  
Robert W. Owen
Keyword(s):  
Dry Weight ◽  
Polyphenolic Compounds ◽  
Weight Basis ◽  
Minor Components ◽  
Methanol Extracts ◽  
Phytochemical Composition ◽  
Lack Of Information

Until recently, there was a lack of information on the phytochemical composition of the taxon Senna. Recent reports show that Senna splendida, Senna gardneri, and Senna georcica are characterized by profiles dominated by flavonoids, naphthapyrones, and stilbenes. Here, we studied the phytochemical composition of Senna macranthera var pudibunda (Benth) Irwin & Barneby for comparison. A total of 26 polyphenolic compounds were identified in methanol extracts of various botanical parts of Senna macranthera var pudibunda (Benth.) Irwin & Barneby. The higher concentration (on a dry weight basis) of polyphenolic compounds was detected in the leaves (48.55 g/kg), bark (21.26 g/kg), and roots (17.08 g/kg), whereas a lower concentration of polyphenolic compounds was detected in the fruits (6.67 g/kg). The polyphenolic profiles of the various botanical parts were dominated by flavan-3-ols and flavan-3-ol dimers. The bark and roots contained only these components (100%), whereas flavonoids were also identified as minor components in the leaves (15.7%) and the fruits (5.6%), respectively. The flavan-3-ol conjugates comprised the well-known procyanidins, along with the rarely reported afzelechin, giobourtinidol and procassinidin dimers.

scholarly journals Characterization and Quantitation of Polyphenolic Compounds in Senna splendida from the Northeast of Brazil

2018 ◽  
Vol 13 (6) ◽  
pp. 1934578X1801300
Author(s):  
Irvila Ricarte de O. Maia ◽  
Maria Teresa Salles Trevisan ◽  
Maria Goretti de V. Silva ◽  
Karel Douglas Klika ◽  
Edy de Brito ◽  
...  
Keyword(s):  
Phenolic Acids ◽  
Dry Weight ◽  
Polyphenolic Compounds ◽  
Weight Basis ◽  
Methanol Extracts

A total of 27 polyphenolic compounds were detected, identified and quantitated in methanol extracts of various botanical parts of Senna splendida (Vogel) H.S. Irwin & Barneby. On a dry weight basis, the higher concentration of polyphenolic compounds was detected in the leaves (28.524 g/kg) and roots (13.884 g/kg). By contrast, the values in the flowers (6.331g/kg) and bark of Senna splendida (2.736 g/kg) were considerably lower. The major component in Senna splendida leaves was quercetin diglucoside, (7.887 g/kg), in the roots methoxy oxyresveratrol (4.485 g/kg), in the flowers quercetin-3- O-rhamnoside-4′- O-glucoside (2.885 g/kg), and in the bark quercitrin (quercetin rhamnoside; 0.881 g/kg). The composition of the polyphenolic compounds in the flowers, leaves and bark were dominated by flavonoids at > 98%, whereas the composition was quite different in the roots, of which the major components were napththapyrones (54 %) and stilbenes (39 %) with a very low contribution from flavonoids (5 %) and phenolic acids (2 %).

scholarly journals Comparative Estimation of Major Iridoid Glucosides from Different Parts of Incarvillea emodi

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Ajay Rana ◽  
Harsh Pratap Singh ◽  
Devendra Dhyani
Keyword(s):  
Calibration Curve ◽  
Limit Of Detection ◽  
Dry Weight ◽  
Weight Basis ◽  
Rich Source ◽  
Linear Calibration ◽  
Limit Of Quantification ◽  
Complete Dominance ◽  
Iridoid Glucosides ◽  
Different Parts

Incarvillea emodi (Bignoniaceae) is a rich source of bioactive iridoid glucosides. This plant contains two major iridoid glucosides: plantarenaloside, a neurotropic compound, and boschnaloside, a strong antibacterial compound. Here, in this study we have developed a simple and fast HPLC-DAD method for the total comparative estimation of these two major iridoids from different parts of Incarvillea emodi. A linear calibration curve (r2=0.999) for both iridoid glucosides in varying range (15.6–500 μg/ml) is obtained. The limit of detection (LOD) and limit of quantification (LOQ) for plantarenaloside were 11.4 ng and 38 ng and for boschnaloside were 22.8 ng and 76 ng, respectively. The shoots, roots, and flowers of Incarvillea emodi have a combined presence of 7.66, 1.22, and 6.99 percent of these iridoid glucosides on dry weight basis. In shoots, plantarenaloside shows complete dominance (6.78%) over boschnaloside (0.88%), and a reversal of this trend was observed in case of flowers where boschnaloside shows complete dominance (6.12%) over plantarenaloside (0.87%). The roots contain 1.19% and 0.03% of both iridoids, respectively.

scholarly journals Evaluation of extracts of wild Cannabis sativa L. for genotoxicity and phytochemical composition

Caryologia ◽  
2021 ◽  
Vol 74 (1) ◽  
pp. 135-150
Author(s):  
Asita Okorie Asita ◽  
Sibusisiwe Magama ◽  
Thato Mamoroesi Moahloli ◽  
Selometsi Baholo
Keyword(s):  
Ethyl Acetate ◽  
Methanol Extract ◽  
Cannabis Sativa ◽  
Ethyl Acetate Extract ◽  
Dry Weight ◽  
Negative Control ◽  
Methanol Extracts ◽  
Phytochemical Composition ◽  
Ethylmethane Sulphonate ◽  
Chromosome Fragments

Cannabis sativa L. is used as medicine and narcotic in Lesotho. Phytochemical composition and total phenolics content (TPC) for hexane, chloroform, ethyl acetate and methanol extracts of aerial parts of C. sativa were determined. Ethyl acetate extract (0.1875, 0.375 and 0.75 mg mL-1) and methanol extract (0.75, 1.5 and 3.0 mg mL-1) were evaluated for cytotoxicity, genotoxicity and modulation of cyclophosphamide (CP, 1.25 mg mL-1)- and ethylmethane sulphonate (EMS, 0.25 mg mL-1)-induced genotoxicity using Allium cepa root meristem assay. CP or EMS did not reduce mitotic index (MI) of cells, hence not cytotoxic when compared with negative control using the t-test (p>0.05), but genotoxic. Both extracts were genotoxic with methanol extract also being cytotoxic. Genotoxicity was the number of aberrant cells per 100 mitotic cells. Modulatory effect (ME) was obtained by comparing mutagen-induced genotoxicity with mixture-induced genotoxicity and expressed as the number of units of mutagen-induced genotoxicity that equalled the mixture-induced genotoxicity. ME was either positive or negative and significant only if ME = ≥ 2. Both extracts were genotoxic with methanol extract also being cytotoxic. Aberrations observed were sticky chromosomes, c-metaphase, anaphase and telophase bridges, chromosome fragments and laggards. Mixture of methanol extract with CP or EMS was more genotoxic (+ME range = 1.61-11.89) than the mutagen or extract alone which suggested synergistic interaction. Mixture of ethyl acetate extract with CP induced insignificant +ME. Mixture of ethylacetate extract with EMS was significantly more genotoxic (+ME = 2.20) than EMS only at high extract concentration. The methanol and ethylacetate extracts of C. sativa were not anti-genotoxic to CP- or EMS- induced genotoxicity. TPCs for hexane, chloroform, ethyl acetate and methanol extracts were 39831.46, 2544.94, 2438.20 and 56601.12 mg GAE/gram dry weight respectively. The differences in the cytotoxicity and MEs of the extracts were attributed to differences in phytochemical composition of extracts.

scholarly journals Phytochemical Composition and Antioxidant Capacity of Three Malian Medicinal Plant Parts

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
François Muanda ◽  
Donatien Koné ◽  
Amadou Dicko ◽  
Rachid Soulimani ◽  
Chafique Younos
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Dry Weight ◽  
Polyphenolic Compounds ◽  
Total Phenolic ◽  
Uv Detector ◽  
Total Anthocyanin ◽  
Plant Parts ◽  
Phytochemical Composition ◽  
Rp Hplc

This study evaluates the levels of total polyphenolic compounds in three Malian medicinal plants and determines their antioxidant potential. Quantitative and qualitative analysis of polyphenolics contained in plants extracts were carried out by RP-C18 RP–HPLC using UV detector. The antioxidant activity was determined by three tests. They are phosphomolybdenum, DPPH (2,2-diphenyl-1 picrylhydrazyl) and ABTS [2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic)] tests. The total phenolic and the total flavonoid contents varied from 200 to 7600 mg 100 g−1dry weight (dw), expressed as gallic acid equivalents and from 680 to 12 300 mg 100 g−1dw expressed as catechin equivalents, respectively. The total anthocyanin concentrations expressed as cyanin-3-glycoside equivalent varied from 1670 to 28 388 mg 100 g−1dw. The antioxidant capacity was measured by determining concentration of a polyphenolic (in mg ml−1) required to quench the free radicals by 50% (IC50) and expressed as vitamin C equivalent antioxidant capacity. The IC50values were ranked between 2.68 and 8.80 μg ml−1of a solution of 50% (v/v) methanol in water. The uses of plants are rationalized on the basis of their antioxidant capacity.

ERRORS ASSOCIATED WITH HERBAGE DISSECTION ANALYSES 2. Problems of species identification

1976 ◽  
pp. 213-225
Author(s):  
I.M. Ritchie ◽  
C.C. Boswell ◽  
A.M. Badland
Keyword(s):  
New Zealand ◽  
Dry Weight ◽  
Analytical Tool ◽  
Species Level ◽  
Weight Basis ◽  
Plant Identification ◽  
Leaf Fragment ◽  
Research Division ◽  
Or Groups ◽  
Botanical Analysis

HERBACE DISSECTION is the process in which samples of herbage cut from trials are separated by hand into component species. Heavy reliance is placed on herbage dissection as an analytical tool ,in New Zealand, and in the four botanical analysis laboratories in the Research Division of the Ministry of Agriculture and Fisheries about 20 000 samples are analysed each year. In the laboratory a representative subsample is taken by a rigorous quartering procedure until approximately 400 pieces of herbage remain. Each leaf fragment is then identified to species level or groups of these as appropriate. The fractions are then dried and the composition calculated on a percentage dry weight basis. The accuracy of the analyses of these laboratories has been monitored by a system of interchanging herbage dissection samples between them. From this, the need to separate subsampling errors from problems of plant identification was, appreciated and some of this work is described here.

Polycyclic Aromatic Hydrocarbons (PAHs) in Sediments of the Brisbane River (Australia) – Preliminary Results

1989 ◽  
Vol 21 (2) ◽  
pp. 161-165 ◽  
Author(s):  
S. I. Kayal ◽  
D. W. Connell
Keyword(s):  
Polycyclic Aromatic Hydrocarbons ◽  
Aromatic Hydrocarbons ◽  
River Estuary ◽  
Dry Weight ◽  
Weight Basis ◽  
Sediment Samples ◽  
Preliminary Results ◽  
Treated Sewage ◽  
Polycyclic Aromatic ◽  
Petroleum Refineries

Results of the analysis of twenty-three composite sediment samples revealed that PAHs are widely distributed in the Brisbane River estuary. Mean concentrations for individual compounds, on a dry weight basis, ranged from 0.03 µg/g for dibenz [ah] anthracene to 2.34 µg/g for fluoranthene. Observed PAH assemblages were rich in compounds having pyrolytic origins. However, the presence of petroleum derived compounds was indicative of the importance of petroleum as a PAH source in the estuary. Petroleum refineries, a coal loading terminal and a major treated sewage outfall located at the mouth were not indicated as major contributing sources of PAH pollution in the estuary.

Mineral nutrition, dinitrogen fixation, and growth of Alnuscrispa and Alnusglutinosa

1985 ◽  
Vol 15 (5) ◽  
pp. 855-861 ◽  
Author(s):  
G. Prégent ◽  
C. Camiré
Keyword(s):  
Mineral Nutrition ◽  
Dry Weight ◽  
Optimal Growth ◽  
Maximum Growth ◽  
Dinitrogen Fixation ◽  
Weight Basis ◽  
Critical Levels ◽  
N Status

Invitro cultures of Alnuscrispa (Ait.) Pursh and Alnusglutinosa (L.) Gaertn. were used to estimate critical foliage levels of selected nutrients for optimal growth and dinitrogen (N2) fixation. For A. crispa to obtain 90% of maximum growth and N2 fixation, foliar levels of 0.12% P, 0.13% Mg, <0.31% K, and <0.04% Ca on a dry weight basis were needed. For A. glutinosa, the critical levels were 0.138% P, 0.10% Mg, 0.29% Ca, and ~0.20% K. From all the deficiencies observed, P had the more pronounced effects on N status of both species.

SOLUBLE SUGAR CHANGES OCCURRING DURING COLD HARDENING OF SPRING WHEAT, FALL RYE AND ALFALFA

1983 ◽  
Vol 63 (2) ◽  
pp. 415-420 ◽  
Author(s):  
D. G. GREEN
Keyword(s):  
Spring Wheat ◽  
Soluble Sugar ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Weight Basis ◽  
Cold Hardening ◽  
Spring Type

Alfa, a relatively nonhardy alfalfa cultivar continued to accumulate, on a dry weight basis, fructose, α- and β-D-glucose, sucrose and maltose during the latter stages of cold hardening. Rambler, a hardier alfalfa cultivar conversely showed a decrease for these soluble sugars with hardening. Frontier rye, a very hardy winter habit cereal showed decreases in these soluble sugars plus melibiose during the same hardening period. These results support the hypothesis that hardy cereals and alfalfa undergo a decrease in soluble sugars with hardening, while less hardy cereals and alfalfa continue to increase in content of soluble sugars. Manitou wheat appeared not to fit this hypothesis and showed the decreased soluble sugars usually associated with hardy cultivars. Although Manitou is a spring type wheat, one of its parents, Thatcher, does contain gene(s) for the winter habit.Key words: Sugar, cold hardening, wheat, rye, alfalfa

scholarly journals THE MOLECULAR WEIGHT OF RHODOPSIN AND THE NATURE OF THE RHODOPSIN-DIGITONIN COMPLEX

1954 ◽  
Vol 37 (3) ◽  
pp. 381-399 ◽  
Author(s):  
Ruth Hubbard
Keyword(s):  
Molecular Weight ◽  
Single Molecule ◽  
Extinction Coefficient ◽  
Dry Weight ◽  
Weight Basis ◽  
Sedimentation Behavior ◽  
A Value ◽  
Wet Weight ◽  
Single Boundary ◽  
Stoichiometric Complex

The sedimentation behavior of aqueous solutions of digitonin and of cattle rhodopsin in digitonin has been examined in the ultracentrifuge. In confirmation of earlier work, digitonin was found to sediment as a micelle (D-1) with an s20 of about 6.35 Svedberg units, and containing at least 60 molecules. The rhodopsin solutions sediment as a stoichiometric complex of rhodopsin with digitonin (RD-1) with an s20 of about 9.77 Svedberg units. The s20 of the RD-1 micelle is constant between pH 6.3 and 9.6, and in the presence of excess digitonin. RD-1 travels as a single boundary also in the electrophoresis apparatus at pH 8.5, and on filter paper at pH 8.0. The molecular weight of the RD-1 micelle lies between 260,000 and 290,000. Of this, only about 40,000 gm. are due to rhodopsin; the rest is digitonin (180 to 200 moles). Comparison of the relative concentrations of RD-1 and retinene in solutions of rhodopsin-digitonin shows that RD-1 contains only one retinene equivalent. It can therefore contain only one molecule of rhodopsin with a molecular weight of about 40,000. Cattle rhodopsin therefore contains only one chromophore consisting of a single molecule of retinene. It is likely that frog rhodopsin has a similar molecular weight and also contains only one chromophore per molecule. The molar extinction coefficient of rhodopsin is therefore identical with the extinction coefficient per mole of retinene (40,600 cm.2 per mole) and the E(1 per cent, 1 cm., 500 mµ) has a value of about 10. Rhodopsin constitutes about 14 per cent of the dry weight, and 3.7 per cent of the wet weight of cattle outer limbs. This corresponds to about 4.2 x 106 molecules of rhodopsin per outer limb. The rhodopsin content of frog outer limbs is considerably higher: about 35 per cent of the dry weight, and 10 per cent of the wet weight, corresponding to about 2.1 x 109 molecules per outer limb. Thus the frog outer limb contains about five hundred times as much rhodopsin as the cattle outer limb. But the relative volumes of these structures are such that the ratio of concentrations is only about 2.5 to 1 on a weight basis. Rhodopsin accounts for at least one-fifth of the total protein of the cattle outer limb; for the frog, this value must be higher. The extinction (K500) along its axis is about 0.037 cm.2 for the cattle outer limb, and about 0.50 cm.2 for the frog outer limb.

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