scholarly journals Chemical Composition and Antibacterial Activity of the Essential Oil Isolated From Flos Lonicerae (Flower Buds of Lonicera macranthoides Hand.-Mazz.)

2021 ◽  
Vol 16 (4) ◽  
pp. 1934578X2110083
Author(s):  
Sun Tao ◽  
Shi Yun ◽  
Mao Yinxue ◽  
Yang Xin ◽  
Qin Kunming
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Antimicrobial Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Flower Buds ◽  
Chinese Medicinal Herb ◽  
Flos Lonicerae ◽  
Lonicera Macranthoides

Flos Lonicerae (FL, flower buds of Lonicera macranthoides Hand.-Mazz.) is a traditional Chinese medicinal herb that is officially listed in the Chinese Pharmacopoeia. The aim of this study was to screen the chemical composition and to study the antibacterial activity of essential oils of Flos Lonicerae. The chemical composition of the essential oils was investigated using gas chromatography-mass spectrometry (GC-MS). The antibacterial activity was evaluated by the disc diffusion method to determine minimum inhibitory concentration (MIC). The major compounds of Flos Lonicerae essential oils were linalool (10.4%), palmitic acid (8.0%), geraniol (6.9%), hexanal (2.5%), and α-terpineol (2.2%). Flos Lonicerae essential oils demonstrated antibacterial activity against Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. The results of this study suggest that the essential oils of Flos Lonicerae have an interesting antimicrobial effect and may be a new potential source for a natural antimicrobial applied in the pharmaceutical field.

Chemical composition and antibacterial activities of Juniperus horizontalis essential oil

2014 ◽  
Vol 94 (2) ◽  
pp. 323-327 ◽  
Author(s):  
T. Eryiğit ◽  
N. Okut ◽  
K. Ekici ◽  
B. Yildirim
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Inhibition Zone ◽  
Antibacterial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Bacterial Strains ◽  
The Subject

Eryiğit, T., Okut, N., Ekici, K. and Yildirim, B. 2014. Chemical composition and antibacterial activities of Juniperus horizontalis essential oil. Can. J. Plant Sci. 94: 323–327. In recent years, the screening of antibacterial activity has been the subject of much research, and the antibacterial activity of essential oils could be a promising subject for future investigation. In this study, the antibacterial activities of Juniperus horizontalis essential oils and their components were investigated. Essential oils were isolated using the hydro-distillation method and their components were analyzed by gas chromatography–mass spectrometry (GC-MS). The main compounds found in the oil of leaves were linalool (33.76%), P-cymene (23.20%), gamma-terpinene (8.67%), trans-sabinene hydrate (8.59%), thyme camphor (8.49%), carvol (5.08%) and borneol (4.22%). Juniperus horizontalis essential oils were evaluated for antibacterial activity against six bacterial strains using the disc diffusion method. The results indicate that the essential oil of J. horizontalis shows variable and significant antibacterial activities against the six tested bacteria species. The diameters of the inhibition zones formed for bacteria were measured. The lowest inhibition zone was 12 mm (sensitive) against Pseudomonas aeruginosa ATCC 10145 and the highest zone was 32 mm (extremely sensitive) against Enterococcus faecalis ATCC 29212.

scholarly journals CHEMICAL COMPOSITION OF ESSENTIAL OILS FROM VITEX AGNUS-CASTUS L. GROWING IN MOROCCO AND ITS IN VITRO ANTIBACTERIAL ACTIVITY AGAINST CLINICAL BACTERIA RESPONSIBLE FOR NOSOCOMIAL INFECTIONS

2018 ◽  
Vol 11 (10) ◽  
pp. 365 ◽  
Author(s):  
Fatima El Kamari ◽  
Amal Taroq ◽  
Yassine El Atki ◽  
Imane Aouam ◽  
Badiaa Lyoussi ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Nosocomial Infections ◽  
University Hospital ◽  
Mass Spectrometry Analysis ◽  
Diffusion Method ◽  
Strong Activity ◽  
Agnus Castus

Objective: The aim of the current study is to determine the chemical composition and evaluate antibacterial activity of Vitex agnus-castus L. (VAC) essential oils against some bacteria causing nosocomial infections in the neonatal and intensive care rooms at the university hospital center of Fez Morocco. Methods: The phytochemical screening of essential oils was determined using gas chromatography (GC) and GC-mass spectrometry analysis. The antibacterial test was evaluated against Gram-positive bacteria Staphylococcus aureus and Gram-negative bacteria species (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) using disc diffusion method. Results: Twenty-nine components were identified in the fruits’ oil representing 93.1% of total oil. The major components in the fruits oil are 1,8-cineole (11.6%), α-thujene (9.3%), phyllocladene (8.2%), α-pinene (7.9%), caryophyllene (5.9%), and cubenol (5%). Furthermore, 28 components were identified in the leaf essential oil. The main component was caryophyllene (9.5%), followed by 1,8-cineole (8.7%), manoyl oxide (7.3%), eugenyl acetate (7.1%), phyllocladene (6.8%), and α-pinene (5.2%). Antibacterial activity of both oils showed a strong activity against nosocomial bacteria tested. Conclusion: Essential oils of Moroccan VAC could be exploited as natural drugs for bacteria, especially those who have acquired resistance to conventional antibiotics.

Chemical composition and biological activities of leaf and fruit essential oils from Eucalyptus camaldulensis

2017 ◽  
Vol 72 (11-12) ◽  
pp. 483-489 ◽  
Author(s):  
Gulden Dogan ◽  
Nazan Kara ◽  
Eyup Bagci ◽  
Seher Gur
Keyword(s):  
Gas Chromatography ◽  
Chemical Composition ◽  
Essential Oils ◽  
Biological Activities ◽  
Eucalyptus Camaldulensis ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Leaf Oil ◽  
Fruit Oil ◽  
Antibacterial And Antifungal

AbstractThe chemical composition of the essential oils from the leaves and fruit ofEucalyptus camaldulensisgrown in Mersin, Turkey was analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) techniques. The biological activities (antibacterial and antifungal) were examined using the agar well diffusion method. The main leaf oil constituents werep-cymene (42.1%), eucalyptol (1,8-cineole) (14.1%), α-pinene (12.7%) and α-terpinol (10.7%). The main constituents of the fruit oil were eucalyptol (1,8-cineole) (34.5%),p-cymene (30.0%), α-terpinol (15.1%) and α-pinene (9.0%). Our results showed that both types of oils are rich in terms of monoterpene hydrocarbons and oxygenated monoterpenes. The leaf and fruit essential oils ofE. camaldulensissignificantly inhibited the growth of Gram-positive (Staphylococcus aureusandBacillus subtilis) and Gram-negative (Escherichia coliandStreptococcussp.) bacteria (p<0.05). The oils also showed fungicidal activity againstCandida tropicalisandC. globrata. Leaf essential oils showed more activity than fruit essential oils, probably due to the higher p-cymene concentration in leaves.

scholarly journals Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria

2019 ◽  
Vol 20 (10) ◽  
Author(s):  
Souheila Bounab ◽  
Takia Lograda ◽  
Messaoud Ramdani ◽  
Pierre Chalard ◽  
Gilles Figueredo
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Diffusion Method ◽  
Nonanoic Acid ◽  
Upgma Cluster Analysis ◽  
Oil Concentration ◽  
Thymelaea Hirsuta ◽  
Main Components

Abstract. Souhila B, Takia L, Messaoud R, Pierre C, Gilles F. 2019. Chemical composition and antibacterial activity of essential oils of Thymelaea hirsuta from Algeria. Biodiversitas 20: 2868-2876. The objectives of this study were to determine the chemical composition and to evaluate the antibacterial activity of Thymelaea hirsuta (L.) Endl., essential oils from seven sampling locations in M'sila region (Algeria). Extraction of essential oils was carried out by the hydro-distillation; the analysis of chemical composition of essential oil was carried out by GC-MS. Antimicrobial activity was performed by disc diffusion method at the essential oil concentration of non-diluted and diluted (1:2, 1:4 and 1:8 v:v of DMSO) against eight species of bacteria. The results showed that the average yields of essential oils were 0.3 ± 0.12%. A total of 45 components were identified, averaging 98.2 ± 1.85% of the total oils. The main components were nonanal-n (10.39 ± 3.21%), hexadecanoic acid (9.77 ± 2.81%), nonanoic acid (9.13 ± 6.49%), triacontane (7.2 ± 3.34%), isopropyl tetradecanoate (6.16 ± 1.99%) and tridecane (4.87 ± 3.1%). Based on the UPGMA cluster analysis, there were two clades of T. hirsuta. T. hirusta has a chemical polymorphism with different chemotypes marked in nature. There were four chemotypes identified in the essential oil of T. hirsuta in the region of M'sila. The essential oil of T. hirsuta has antibacterial activity against eight tested bacteria on the concentration-dependentt manner.

scholarly journals Molecular Composition and Antibacterial Effect of Five Essential Oils Extracted from Nigella sativa L. Seeds against Multidrug-Resistant Bacteria: A Comparative Study

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Mohammed Dalli ◽  
Salah-eddine Azizi ◽  
Hind Benouda ◽  
Ali Azghar ◽  
Maroua Tahri ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Nigella Sativa ◽  
Multidrug Resistant ◽  
Climatic Conditions ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Microdilution Method

Nigella sativa L. (NS) and its volatile compounds are well known for their broad spectrum of effects. This study aimed to investigate the variability of the chemical composition and the in vitro antibacterial activity of five essential oils (Eos) originated from Morocco, Saudi Arabia, Syria, India, and France. These five samples were grown under different edaphic and climatic conditions. The agar diffusion method and microdilution method in 96-well plates were used to test the sensitivity of multidrug-resistant strains clinically isolated from patients (methicillin-resistant Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Acinetobacter baumannii), for the determination of the minimum inhibitory concentration and bactericidal concentration. Among all the investigated Eos, the monoterpenes were highly present in the chemical composition. Moroccan, Saudi Arabian, and Syrian seeds were characterized by the presence α-phellandrene (20.03–30.54%), β-cymene (12.31–23.82 %), and 4−caranol (9.77–14.27%). The Indian seeds were rich with 4-caranol (18.81%), β-cymene (14.22%), α-phellandrene (10.58%), and β-chamigrene (9.54%), while France NS was rich with estragole (20.22%) and D-limonene (14.63%). The minimum inhibitory (MIC) and bactericidal concentration (MBC) obtained for the four Eos (with the exception of France because of the low yield) tested were ranging from 3 to 40 μl/ml. Gram-positive (+) bacteria were slightly sensitive to the Eos tested than the Gram-negative (−) bacteria. The results of this study showed that the Eos of NS seeds show interesting antibacterial activity which could be associated to the existence of different bioactive compounds. Indeed, these compounds can be used for preventive or curative purposes in the face of the noncontrolled emergence of resistance to antibiotics.

Chemical composition, antioxidant, and antimicrobial activities of two essential oils from Algerian propolis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Safia Boulechfar ◽  
Amar Zellagui ◽  
Meltem Asan-Ozusaglam ◽  
Chawki Bensouici ◽  
Ramazan Erenler ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oils ◽  
Pathogenic Bacteria ◽  
Antioxidant Properties ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Reducing Ability ◽  
Antioxidant And Antimicrobial Activity ◽  
Fungistatic Effect

Abstract This study aims to investigate the chemical composition, antioxidant, and antimicrobial activity of two essential oils (EOs) from Algerian propolis. The volatile constituents were analyzed by gas chromatography-mass spectrometry. Fifty components were identified from the oils. The major components were found to be: cedrol (17.0%), β-eudesmol (7.7%), and α-eudesmol (6.7%) in EO of propolis from Oum El Bouaghi (EOPO) whilst α-pinene (56.1%), cis-verbenol (6.0%), and cyclohexene,3-acetoxy-4-(1-hydroxy-1-methylethyl)-1-methyl (4.4%) in EO of propolis from Batna (EOPB). The antioxidant properties of EOPO and EOPB were determined using 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS•+) and cupric reducing antioxidant capacity (CUPRAC assays), respectively. Both EOs had more cupric ion reducing ability than scavenging ABTS•+ radicals. The antimicrobial potential of the two EOs against eight pathogens was assayed by the agar diffusion method and the mode of action was determined by microdilution assay. The results revealed that EOPB was bactericidal for all tested pathogenic bacteria and fungicidal for Candida albicans ATCC 10231, whereas, EOPO showed bacteriostatic effect against Escherichia coli O157:H7 and Pseudomonas aeruginosa ATCC27853 and fungistatic effect against C. albicans ATCC 10231. Thus, the obtained results suggest the important use of propolis EOs as preservative agents.

scholarly journals In vitro Study of Antibacterial Activity on Multi-Resistant Bacteria and Chemical Composition of the Chloroform Extract of Endemic Centaurea drabifolia subsp. cappadocica

2009 ◽  
Vol 4 (9) ◽  
pp. 1934578X0900400 ◽  
Author(s):  
Aysel Ugur ◽  
Nurdan Sarac ◽  
M. Emin Duru ◽  
Yavuz Beyatli
Keyword(s):  
Gas Chromatography ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
In Vitro Study ◽  
Chloroform Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
Ethyl Palmitate

The antimicrobial activity of the n-hexane, chloroform, ethyl acetate and ethanol extracts of the aerial parts of C. drabifolia S.M. subsp. cappadocica (DC.) Wagenitz (Asteraceae) was evaluated against microorganisms including multi-antibiotic resistant bacteria using the paper disc diffusion method. The chemical composition of the chloroform extract of this plant was determined by gas chromatography and gas chromatography-mass spectrometry. The chloroform extract exhibited significant antibacterial activity against all the bacteria tested, except Stenotrophomonas maltophilia MU63. The major compounds of the chloroform extract were spathulenol (14.1%), caryophyllene oxide (12.5%), octadecanol (10.2%), ethyl palmitate (7.7%), [Z,Z]-10,12-hexadecadienal (6.0%), 3-hydroxy p-anisaldehyde (5.9%) and pentacosane (5.8%).

scholarly journals Chemical composition, antimicrobial activity and chromosome number of Hertia cheirifolia L. from Algeria

2020 ◽  
Vol 7 (2) ◽  
pp. 31-43
Author(s):  
Ounoughi Abdelkader ◽  
Ramdani Messaoud ◽  
Lograda Takia ◽  
Chalard Pierre ◽  
Figueredo Gilles
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Chromosome Number ◽  
Essential Oil ◽  
Essential Oils ◽  
Diffusion Method ◽  
Chemical Compositions ◽  
Root Cells ◽  
Karyotypic Analysis ◽  
Germacrene D

AbstractThe aims of this work are to investigate the chemical composition, the antibacterial activity of the essential oil and the chromosome numbers of two populations of Hertia cheirifolia. The samples were collected in the flowering stage, in eastern Algeria locality. The aerial parts of H. cheirifolia were submitted to a hydro-distillation. GC and GC / MS analysed the chemical compositions of the obtained essential oils. The antibacterial activity of essential oils was evaluated using the disks diffusion method against ten bacterial strains. For karyotypic analysis, the squashing method is used. Fifty-eight compounds representing 98.93% of the total oil were identified in H. cheirifolia. The chemical composition is dominated by the presence of major products, α-pinene (48.49 - 53.85%) and Germacrene-D (2.64 - 12.66%). Two distinct chemical breeds were identified, the α-pinene-spathulenol of Batna population, and the α-pinene-germacrene-D of Setif population. The essential oil of H. cheirifolia has a moderate activity against bacteria tested. In contrast, the strains E. coli ATCC 25922, P. syringae ATCC 53543 and E. fecalis ATCC 49452 are resistant to H. cheirifolia essential oils. The observations of root cells meristematic at metaphase of H. cheirifolia gave a diploid chromosome number 2n = 2x = 20, with a basic chromosome number (x= 10).

scholarly journals Chemical Composition and Antibacterial Activity of Essential Oils from the Algerian Endemic Origanum glandulosum Desf. against Multidrug-Resistant Uropathogenic E. coli Isolates

Antibiotics ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 29 ◽  
Author(s):  
Larbi Zakaria Nabti ◽  
Farida Sahli ◽  
Hocine Laouar ◽  
Ahmed Olowo-okere ◽  
Joice Guileine Nkuimi Wandjou ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Resistant Bacteria ◽  
E Coli ◽  
Broth Microdilution Method ◽  
Resistant Strains ◽  
Tract Infections

Antibiotics are becoming ineffective against resistant bacteria. The use of essential oils (EOs) may constitute an alternative solution to fight against multidrug-resistant bacteria. This study aims to determine the chemical composition of EOs from five populations of the endemic Algerian Origanum glandulosum Desf. and to investigate their potential antibacterial activity against multidrug-resistant uropathogenic E. coli strains. The EOs were obtained by hydrodistillation and their composition was investigated by gas chromatography/mass spectrometry (GC/MS). The antibacterial activity was evaluated by the disc diffusion method against eight E. coli strains (six uropathogenic resistant and two referenced susceptible strains). Minimum inhibitory and bactericidal concentrations (MIC/MBC) were obtained by the broth microdilution method. The main EO components were thymol (15.2–56.4%), carvacrol (2.8–59.6%), γ-terpinene (9.9–21.8%) and p-cymene (8.5–13.9%). The antibacterial tests showed that all the EOs were active against all the strains, including the multidrug-resistant strains. The EO from the Bordj location, which contained the highest amount of carvacrol (59.6%), showed the highest antibacterial activity (inhibition diameters from 12 to 24.5 mm at a dilution of 1/10). To our knowledge, this is the first description of the activity of O. glandulosum EOs against resistant uropathogenic strains. Our study suggests that O. glandulosum EO could be used in some clinical situations to treat or prevent infections (e.g., urinary tract infections) with multidrug-resistant strains.

scholarly journals Chemical Composition, Antioxidant, and Antimicrobial Activities of the Essential Oils From Аrtemisia annua L. Growing Wild in Tajikistan

2020 ◽  
Vol 15 (5) ◽  
pp. 1934578X2092781
Author(s):  
Farukh S Sharopov ◽  
Aminjon Salimov ◽  
Sodik Numonov ◽  
Abduahad Safomuddin ◽  
Mahinur Bakri ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Antioxidant Activity ◽  
Antibacterial Activity ◽  
Chemical Composition ◽  
Essential Oils ◽  
Hierarchical Cluster ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Aerial Parts ◽  
Antioxidant And Antimicrobial Activities

The aerial parts of Аrtemisia annua L. were collected from Varzob, Rudaki, and Hisor regions of Tajikistan. The essential oil was obtained by hydrodistillation and analyzed by gas chromatography–mass spectrometry. The essential oils of aerial parts of А. annua were dominated by the monoterpenoids like camphor (32.5%-58.9%), 1,8-cineole (13.7%-17.8%), camphene (4.5%-8.4%), and α-pinene (1.9%-7.3%). Hierarchical cluster analysis of A. annua essential oils indicated the existence of 3 A. annua chemotypes: camphor/1,8-cineole, camphor, and artemisia ketone. The essential oils of А. annua show weak antioxidant activity and average antibacterial activity. In our opinion, the antibacterial activity of А. annua essential oils is related to the presence of 1,8-cineole. To our best knowledge, this is the first report concerning the chemical composition, chemotypic variation, antioxidant, and antimicrobial activities of the essential oils obtained from the aerial parts of А. annua, growing wild in Tajikistan.

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