scholarly journals A Preliminary Study of Combined Detection of COMP, TIMP-1, and MMP-3 in Synovial Fluid: Potential Indicators of Osteoarthritis Progression

Cartilage ◽  
2020 ◽  
pp. 194760352094638
Author(s):  
Jana Plsikova Matejova ◽  
Timea Spakova ◽  
Denisa Harvanova ◽  
Marek Lacko ◽  
Vladimir Filip ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Matrix Protein ◽  
Weight Bearing ◽  
Knee Oa ◽  
Significant Linear Correlation ◽  
Starting Point ◽  
Osteoarthritis Progression ◽  
Grading Scale ◽  
Severity Of The Disease ◽  
End Stage

Objective Osteoarthritis (OA) commonly affects weight-bearing joints and is characterized by articular cartilage breakdown combined with osteophyte formation at the joint margins and chronic nonspecific inflammation of synovium. Understanding the profile of inflammation in a patient population is an essential starting point to predict or prevent OA progression. The aim of this study was to identify the profile of selected biomolecules in synovial fluid (SF) and investigate the correlation according to gender, age, and severity of the disease within patients from among the general knee OA population. Design In our study SF samples were aspirated from the knees of 65 OA patients (46 patients with early knee OA and 19 patients with end-stage knee OA according to the Kellgren-Lawrence grading scale). The concentration of interleukins (IL-6, IL-8), matrix metalloproteinases (MMP-1, MMP-3, MMP-13), MMPs inhibitors (TIMP-1, TIMP-2), cartilage oligomeric matrix protein (COMP), and adiponectin was analyzed using a multiplex ELISA-based approach. Conclusions Our results indicate significant linear correlation of MMP-13 and COMP concentration with age ( P < 0.05), but not with OA severity. In fact, 3 of the examined biomolecules, MMP-3 ( P < 0.01), TIMP-1 ( P < 0.01), and COMP ( P < 0.05) significantly correlate with the grade of knee OA and might be associated with OA severity.

scholarly journals Synovial Fluid Fatty Acid Profiles Differ between Osteoarthritis and Healthy Patients

Cartilage ◽  
2018 ◽  
Vol 11 (4) ◽  
pp. 473-478 ◽  
Author(s):  
Arne Van de Vyver ◽  
Stefan Clockaerts ◽  
Chris H. A. van de Lest ◽  
Wu Wei ◽  
Jan Verhaar ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Joint Disease ◽  
Knee Joints ◽  
Nervonic Acid ◽  
Omega 3 ◽  
Knee Oa ◽  
Omega 6 ◽  
History Of ◽  
End Stage ◽  
And Control

Objective Free fatty acids (FAs) may influence cartilage metabolism and osteoarthritis (OA) disease progression. It is not clearly studied which FAs are present in the synovial fluid of knee joints and whether there are differences in FA content between nonsymptomatic and OA knee joints. The aim of this study was to investigate the presence of different types of FAs in synovial fluid of both OA- and nonsymptomatic control joints, and to analyze differences between both groups. Design A total of 23 synovial fluid samples were collected from patients with end-stage knee OA undergoing total knee replacement, with approval of the medical ethical committee. As controls, 6 synovial fluid samples were obtained from postmortem donors without any history of joint disease or arthritis. Measurement of free FA concentration was done by mass spectrometry for saturated FAs (SFA), monounsaturated FAs (MUFA), and omega-3 and omega-6 polyunsaturated FAs (n-3 PUFAs and n-6 PUFAs). Results Our measurements demonstrated the presence of SFAs, MUFAs, n-3 and n-6 PUFAs in synovial fluid of both nonsymptomatic and OA knee joints. The n-6/n-3 ratio was significantly lower in the OA group ( P = 0.0005). Arachidonic acid (n-6 PUFA) concentrations were also lower in OA synovial fluid ( P = 0.01), while tetracosadienoic acid ( P = 0.0001) and nervonic acid ( P = 0.001) (MUFAs) were higher in synovial fluid of patients with knee OA. Conclusion Synovial fluid contains a broad spectrum of free FAs. The FAs profile differs between OA and control subjects, including a tendency for less n-6 FAs in OA joints.

scholarly journals Tibial Condyle Valgus Osteotomy for Ipsilateral Knee Osteoarthritis after Hip Arthrodesis

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Daisuke Fukuhara ◽  
Hiroaki Inoue ◽  
Shuji Nakagawa ◽  
Yuji Arai ◽  
Kenji Takahashi
Keyword(s):  
Knee Joint ◽  
Knee Osteoarthritis ◽  
Mechanical Load ◽  
Weight Bearing ◽  
Valgus Osteotomy ◽  
Hip Arthrodesis ◽  
Knee Oa ◽  
Early Loosening ◽  
Tibial Condyle ◽  
End Stage

We report a case of tibial condylar valgus osteotomy (TCVO) for ipsilateral knee osteoarthritis (OA) after hip arthrodesis. A 58-year-old woman developed right purulent hip arthritis at one month of age and underwent right hip fusion at 16 years old. She visited our department at the age of 57 because her right knee joint pain worsened. The range of motion for her right knee was 80° and -5° of flexion and extension, respectively, and she experienced medial weight-bearing pain. A plain X-ray image showed that the right knee joint had end-stage knee OA with a bone defect inside the tibia, and the tibial plateau shape was the pagoda type. There was a marked instability in her right knee with a valgus of 9° and varus of 7° on stress photography. She underwent TCVO on her right knee and was allowed full load four weeks after surgery. Computed tomography imaging showed bone union nine months after surgery. Two years after the operation, there was no correction loss, and she could walk independently without pain. In general, total knee arthroplasty (TKA) is indicated for end-stage knee OA; however, there are problems, such as early loosening due to the increased mechanical load on the knee after hip OA. In this case, since a good course was obtained, TCVO is considered a treatment option for terminal knee OA after hip arthrodesis.

OXIDATIVE STRESS INCIDENCE ON THE SEVERITY OF KNEE OSTEOARTHRITIS

2017 ◽  
Vol 20 (02) ◽  
pp. 1750008 ◽  
Author(s):  
Stergios A. Oikonomidis ◽  
Yannis V. Simos ◽  
Ioannis K. Toliopoulos ◽  
Ioannis I. Verginadis ◽  
Alexandros S. Oikonomidis ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Synovial Fluid ◽  
Knee Osteoarthritis ◽  
Antioxidant Capacity ◽  
Controlled Trial ◽  
Therapeutic Interventions ◽  
Clinical Markers ◽  
Knee Oa ◽  
Total Antioxidant ◽  
Grading Scale

The aim of the study was to examine the incidence of oxidative stress on the severity of knee osteoarthritis (OA). Data were obtained from a previous pilot controlled trial among patients, diagnosed with OA in one or both knees, that were randomly assigned into two different treatment groups and were either supplemented with ascorbic acid and Vitamin E daily per os or treated with meloxicam. The following markers were estimated: A. Clinical markers: functionality of the knee (WOMAC index), pain (using a pain visual analogue scale) and severity of OA (Kellgren–Lawrence grading scale) B. Laboratory markers: total antioxidant capacity (TAC) and malonyldialdehyde (MDA) levels in the synovial fluid. The TAC of the knees was moderately correlated with the severity of OA and the level of pain whilst MDA concentration was weekly correlated. An average change of 5[Formula: see text]mM of a-tocopherol in TAC (4.6–5.5[Formula: see text]mM of a-tocopherol) defines the shift among stages of OA and the level of pain experienced by the patients. Neither TAC nor MDA were correlated with the Kellgren–Lawrence grading scale. Alleviation of oxidative stress should be a key objective for the therapeutic interventions (pharmacological and nonpharmacological) in knee OA. Even small improvements in antioxidant capacity of the synovial fluid may contribute to the patient’s quality of life and to the deceleration of the disease progression.

scholarly journals Automated Classification of Osteoarthritis in the Knee Using Deep Learning

2021 ◽  
Author(s):  
Simon Olsson ◽  
Ehsan Akbarian ◽  
Anna Lind ◽  
Ali Sharif Razavian ◽  
Max Gordon
Keyword(s):  
Neural Network ◽  
Deep Learning ◽  
Weight Bearing ◽  
Operative Management ◽  
University Hospital ◽  
Automated Classification ◽  
Knee Oa ◽  
Orthopedic Surgeons ◽  
Grading Scale ◽  
Visual Disturbances

Abstract BACKGROUNDPrevalence for knee osteoarthritis is rising in Sweden and globally due to an ageing and more obese population. This has subsequently led to an increasing demand for knee arthroplasties. Correctly diagnosing, classifying, follow-up and planning for either conservative or operative management of knee OA is therefore of a great interest. Most orthopedic surgeons rely on standard weight bearing radiographs, improving the reliability and reproducibility of these interpretations could thus be hugely beneficial. Recently deep learning, a form of artificial intelligence (AI), has shown promising results for interpreting radiographs. In this study, we aim to evaluate how well an AI can classify knee OA severity using entire image series and not excluding common visual disturbances such as implants, casts and other pathologies.MethodsWe selected 6103 radiograph exams taken at Danderyd University Hospital between the years 2002-2016 and manually categorized them according to the Kellgren & Lawrence grading scale (KL). We then trained a convolutional neural network that we evaluated against a test set of 300 exams. These exams had been reviewed independently by two senior orthopedic surgeons who settled exams with disagreement through a consensus session. ResultsOur network yielded an overall high AUC of >0.87 for all KL grades except KL grade 2 and a mean AUC of 0.92. When merging adjacent KL grades together, all but one group showed near perfect results with AUC > 0.95 indicating excellent performance. ConclusionWe found that we could teach a neural network classify knee OA severity and laterality using the KL grading scale without cleaning the input data from major visual disturbances such as implants and other pathologies.

scholarly journals Phospholipase A2 inhibitor–loaded micellar nanoparticles attenuate inflammation and mitigate osteoarthritis progression

2021 ◽  
Vol 7 (15) ◽  
pp. eabe6374
Author(s):  
Yulong Wei ◽  
Lesan Yan ◽  
Lijun Luo ◽  
Tao Gui ◽  
Biang Jang ◽  
...  
Keyword(s):  
Clinical Success ◽  
Joint Space ◽  
Therapeutic Agents ◽  
Knee Joints ◽  
Phospholipid Membrane ◽  
Drug Discovery And Development ◽  
Knee Oa ◽  
Clinical Challenge ◽  
Osteoarthritis Progression ◽  
Human And Mouse

Treating osteoarthritis (OA) remains a major clinical challenge. Despite recent advances in drug discovery and development, no disease-modifying drug for knee OA has emerged with any notable clinical success, in part, due to the lack of valid and responsive therapeutic targets and poor drug delivery within knee joints. In this work, we show that the amount of secretory phospholipase A2 (sPLA2) enzyme increases in the articular cartilage in human and mouse OA cartilage tissues. We hypothesize that the inhibition of sPLA2 activity may be an effective treatment strategy for OA. To develop an sPLA2-responsive and nanoparticle (NP)–based interventional platform for OA management, we incorporated an sPLA2 inhibitor (sPLA2i) into the phospholipid membrane of micelles. The engineered sPLA2i-loaded micellar NPs (sPLA2i-NPs) were able to penetrate deep into the cartilage matrix, prolong retention in the joint space, and mitigate OA progression. These findings suggest that sPLA2i-NPs can be promising therapeutic agents for OA treatment.

scholarly journals Analysis of Cartilage Oligomeric Matrix Protein (COMP) in Synovial Fluid, Serum and Urine from 51 Racehorses with Carpal Bone Fracture

2008 ◽  
Vol 70 (9) ◽  
pp. 915-921 ◽  
Author(s):  
Koh ARAI ◽  
Masaaki TAGAMI ◽  
Takashi HATAZOE ◽  
Eikoh NISHIMATSU ◽  
Yuri SHIMIZU ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Bone Fracture ◽  
Cartilage Oligomeric Matrix Protein ◽  
Matrix Protein ◽  
Carpal Bone ◽  
Serum And Urine

scholarly journals One year change of knee cartilage morphology in the first release of participants from the Osteoarthritis Initiative progression subcohort: association with sex, body mass index, symptoms and radiographic osteoarthritis status

2008 ◽  
Vol 68 (5) ◽  
pp. 674-679 ◽  
Author(s):  
F Eckstein ◽  
S Maschek ◽  
W Wirth ◽  
M Hudelmaier ◽  
W Hitzl ◽  
...  
Keyword(s):  
Risk Factors ◽  
Body Mass Index ◽  
Body Mass ◽  
Statistical Significance ◽  
Weight Bearing ◽  
Rate Of Change ◽  
Cartilage Loss ◽  
Knee Oa ◽  
Symptomatic Knee ◽  
Osteoarthritis Initiative

Objective:The Osteoarthritis Initiative (OAI) is a multicentre study targeted at identifying biomarkers for evaluating the progression and risk factors of symptomatic knee OA. Here cartilage loss using 3 Tesla (3 T) MRI is analysed over 1 year in a subset of the OAI, together with its association with various risk factors.Methods:An age- and gender-stratified subsample of the OAI progression subcohort (79 women and 77 men, mean (SD) age 60.9 (9.9) years, body mass index (BMI) 30.3 (4.7)) with both frequent symptoms and radiographic OA in at least one knee was studied. Coronal FLASHwe (fast low angle shot with water excitation) MRIs of the right knee were acquired at 3 T. Seven readers segmented tibial and femoral cartilages blinded to order of acquisition. Segmentations were quality controlled by one expert.Results:The reduction in mean cartilage thickness (ThC) was greater (p = 0.004) in the medial than in the lateral compartment, greater (p = 0.001) in the medial femur (−1.9%) than in the medial tibia (−0.5%) and greater (p = 0.011) in the lateral tibia (−0.7%) than in the lateral femur (0.1%). Multifactorial analysis of variance did not reveal significant differences in the rate of change in ThC by sex, BMI, symptoms and radiographic knee OA status. Knees with Kellgren–Lawrence grade 2 or 3 and with a BMI >30 tended to display greater changes.Conclusions:In this sample of the OAI progression subcohort, the greatest, but overall very modest, rate of cartilage loss was observed in the weight-bearing medial femoral condyle. Knees with radiographic OA in obese participants showed trends towards higher rates of change than those of other participants, but these trends did not reach statistical significance.

scholarly journals High systemic bone mineral density increases the risk of incident knee OA and joint space narrowing, but not radiographic progression of existing knee OA: the MOST study

2009 ◽  
Vol 69 (01) ◽  
pp. 163-168 ◽  
Author(s):  
M C Nevitt ◽  
Y Zhang ◽  
M K Javaid ◽  
T Neogi ◽  
J R Curtis ◽  
...  
Keyword(s):  
Bone Mineral Density ◽  
Bone Mineral ◽  
Joint Space Narrowing ◽  
Weight Bearing ◽  
Joint Space ◽  
Whole Body ◽  
Mineral Density ◽  
Knee Oa ◽  
Increased Risk

Objectives:Previous studies suggest that high systemic bone mineral density (BMD) is associated with incident knee osteoarthritis (OA) defined by osteophytes but not with joint space narrowing (JSN), and are inconsistent regarding BMD and progression of existing OA. The association of BMD with incident and progressive tibiofemoral OA was tested in a large prospective study of men and women aged 50–79 years with or at risk for knee OA.Methods:Baseline and 30-month weight-bearing posteroanterior and lateral knee radiographs were scored for Kellgren-Lawrence (K-L) grade, JSN and osteophytes. Incident OA was defined as the development of K-L grade ⩾2 at follow-up. All knees were classified for increases in grade of JSN and osteophytes from baseline. The association of gender-specific quartiles of baseline BMD with risk of incident and progressive OA was analysed using logistic regression, adjusting for covariates.Results:The mean (SD) age of 1754 subjects was 63.2 (7.8) years and body mass index was 29.9 (5.4) kg/m2. In knees without baseline OA, higher femoral neck and whole body BMD were associated with an increased risk of incident OA and increases in grade of JSN and osteophytes (p<0.01 for trends); adjusted odds were 2.3–2.9-fold greater in the highest compared with the lowest BMD quartiles. In knees with existing OA, progression was not significantly related to BMD.Conclusions:In knees without OA, higher systemic BMD was associated with a greater risk of the onset of JSN and K-L grade ⩾2. The role of systemic BMD in early knee OA pathogenesis warrants further investigation.

scholarly journals Arthrosonography and biomarkers in the evaluation of destructive knee cartilage osteoarthrosis

2009 ◽  
Vol 137 (11-12) ◽  
pp. 653-658 ◽  
Author(s):  
Sandra Zivanovic ◽  
Ljiljana Petrovic-Rackov ◽  
Aleksandar Zivanovic
Keyword(s):  
Matrix Protein ◽  
Biological Fluids ◽  
Bone Destruction ◽  
Cartilage Thickness ◽  
Type I ◽  
Human Cartilage ◽  
Medial Condyle ◽  
Negative Direction ◽  
Knee Oa ◽  
Progressive Loss

Introduction. Knee osteoarthrosis (OA) is a degenerative disease with progressive loss of cartilage of joints and bone destruction. During this process, the release of fragments of connective tissue matrix is detected in the biological fluids such as human cartilage glycoprotein (YKL-40), cartilage oligomeric matrix protein (COMP) and collagen type I C terminal telopeptid (CTX-I). Objective. The aim of the study was to determine the degree of connection cartilage thickness measured by ultrasound with serum concentrations of biomarkers YKL-40, COMP and CTX-I in patients with primary knee OA. Methods. The analysis included 88 patients with the diagnosis of knee OA. Ultrasound examination of knees were done by two rheumatologists. The analysis of serum samples determined the concentration of COMP, YKL-40 and CTX-I by the ELISA method. Results. The average age of patients was 69.97?9.37 years and the duration of knee OA 6.46?6.73 years. The average cartilage thickness of the femoral condyle was 1.33?0.20 mm; of the medial condyle (MC) (front access) 1.30?0.23 mm, (rear access) 1.30?0.29 mm and lateral condyli (LC) (front access) 1.39?0.27 mm. The average cartilage thickness of MC (front access) was 1.27 mm (0.98-1.42 mm), (rear access) 1.27 mm (0.84-1.46 mm) and LC (front access) 1.36 mm (1.01-1.57 mm) (p=0.002). There was a significant connection in the negative direction between the patients' age and the cartilage thickness of MC (front and rear access) and LC (front access) (r=-0.253; p=0.017). There was a significant negative direction of interrelationship between the cartilage thickness of MC (front access) (r=-0.259; p=0.015) and LC (front access) and the disease duration (r=-0.259; p=0.015). In patients with knee OA lasting for 5 years the measured cartilage thickness was 1.27 mm (1.16-1.49 mm), and 0.99 mm (0.94-1.23 mm) (p=0.007) in those lasting for 20 years. There was a significant relationship in a negative direction between the concentration of YKL-40 and cartilage thickness of MC (front access) (r=-0.249; p=0.019). Conclusion. The progressive loss of cartilage during the long-term evolution of osteoarthrosis is most extensive in the femoral MC. The increased serum levels of YKL-40 can be a good indicator of joint cartilage destruction.

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