scholarly journals Silk fibroin induces chondrogenic differentiation of canine adipose–derived multipotent mesenchymal stromal cells/mesenchymal stem cells

2019 ◽  
Vol 10 ◽  
pp. 204173141983505 ◽  
Author(s):  
Metka Voga ◽  
Natasa Drnovsek ◽  
Sasa Novak ◽  
Gregor Majdic
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Stromal Cells ◽  
Chondrogenic Differentiation ◽  
Culture Medium ◽  
Collagen Type ◽  
Multipotent Mesenchymal Stromal Cells ◽  
Culture Conditions ◽  
Standard Cell ◽  
Cell Culture Medium

Under appropriate culture conditions, mesenchymal stem cells (MSC), also called more properly multipotent mesenchymal stromal cells (MMSC), can be induced toward differentiation into different cell lineages. In order to guide stem cell fate within an environment resembling the stem cell niche, different biomaterials are being developed. In the present study, we used silk fibroin (SF) as a biomaterial supporting the growth of MMSC and studied its effect on chondrogenesis of canine adipose–derived MMSC (cADMMSC). Adipose tissue was collected from nine privately owned dogs. MMSC were cultured on SF films and SF scaffolds in a standard cell culture medium. Cell morphology was evaluated by scanning electron microscopy (SEM). Chondrogenic differentiation was evaluated by alcian blue staining and mRNA expression of collagen type 1, collagen type 2, Sox9, and Aggrecan genes. cADMMSC cultured on SF films and SF scaffolds stained positive using alcian blue. SEM images revealed nodule-like structures with matrix vesicles and fibers resembling chondrogenic nodules. Gene expression of chondrogenic markers Sox9 and Aggrecan were statistically significantly upregulated in cADMMSC cultured on SF films in comparison to negative control cADMMSC. This result suggests that chondrogenesis of cADMMSC could occur when cells were grown on SF films in a standard cell culture medium without specific culture conditions, which were previously considered necessary for induction of chondrogenic differentiation.

scholarly journals Exposure Setup and Dosimetry for a Study on Effects of Mobile Communication Signals on Human Hematopoietic Stem Cells in vitro

2017 ◽  
Vol 15 ◽  
pp. 207-213
Author(s):  
Martina Rohland ◽  
Kai Baaske ◽  
Katharina Gläser ◽  
Henning Hintzsche ◽  
Helga Stopper ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Hematopoietic Stem Cells ◽  
Mobile Communication ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Scattering Parameter ◽  
Hematopoietic Stem ◽  
Communication Signals

Abstract. In this paper we describe the design of an exposure setup used to study possible non-thermal effects due to the exposure of human hematopoietic stem cells to GSM, UMTS and LTE mobile communication signals. The experiments are performed under fully blinded conditions in a TEM waveguide located inside an incubator to achieve defined environmental conditions as required for the living cells. Chamber slides containing the cells in culture medium are placed on the septum of the waveguide. The environmental and exposure parameters such as signal power, temperatures, relative humidity and CO2 content of the surrounding atmosphere are monitored permanently during the exposure experiment. The power of the exposure signals required to achieve specific absorption rates of 0.5, 1, 2 and 4 W kg−1 are determined by numerical calculation of the field distribution inside the cell culture medium at 900 MHz (GSM), 1950 MHz (UMTS) and 2535 MHz (LTE). The dosimetry is verified both with scattering parameter measurements on the waveguide with and without containers filled with cell culture medium and with temperature measurements with non-metallic probes in separate heating experiments.

scholarly journals Human Adipose Tissue Derived Stem Cells Promote Liver Regeneration in a Rat Model of Toxic Injury

2013 ◽  
Vol 2013 ◽  
pp. 1-10 ◽  
Author(s):  
Eva Koellensperger ◽  
Willem Niesen ◽  
Jonas Kolbenschlag ◽  
Felix Gramley ◽  
Guenter Germann ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Adipose Tissue ◽  
Liver Regeneration ◽  
Culture Medium ◽  
Human Adipose Tissue ◽  
Cell Culture Medium ◽  
Sprague Dawley ◽  
Promising Alternative ◽  
Histological Sections

In the light of the persisting lack of donor organs and the risks of allotransplantations, the possibility of liver regeneration with autologous stem cells from adipose tissue (ADSC) is an intriguing alternative. Using a model of a toxic liver damage in Sprague Dawley rats, generated by repetitive intraperitoneal application of retrorsine and allyl alcohol, the ability of human ADSC to support the restoration of liver function was investigated. A two-thirds hepatectomy was performed, and human ADSC were injected into one remaining liver lobe in group 1 (n= 20). Injection of cell culture medium performed in group 2 (n= 20) served as control. Cyclosporine was applied to achieve immunotolerance. Blood samples were drawn weekly after surgery to determine liver-correlated blood values. Six and twelve weeks after surgery, animals were sacrificed and histological sections were analyzed. ADSC significantly raised postoperative albumin (P< 0.017), total protein (P< 0.031), glutamic oxaloacetic transaminase (P< 0.001), and lactate dehydrogenase (P< 0.04) levels compared to injection of cell culture medium alone. Transplanted cells could be found up to twelve weeks after surgery in histological sections. This study points towards ADSC being a promising alternative to hepatocyte or liver organ transplantation in patients with severe liver failure.

scholarly journals The study of biological activity nanomaterial in cultivation conditions pigs sperm and oocytes in vitro

1970 ◽  
pp. 256-260 ◽  
Author(s):  
O. V. Shcherbak ◽  
A. B. Ziuziun ◽  
O. S. Osypchuk ◽  
S. I. Kovtun ◽  
N. P. Halahan ◽  
...  
Keyword(s):  
Cell Culture ◽  
Biological Activity ◽  
Culture Medium ◽  
Culture Conditions ◽  
Cell Culture Medium ◽  
Animal Genetic Resources ◽  
Cultivation In Vitro ◽  
Breeding And Genetics ◽  
Positive Effect

Aim. To assess the biological activity of nanomaterial UFS/BSA/NANA of culture conditions with different concentrations of spermatozoa and the oocyte-cumulus complexes of pigs in vitro. Methods. For study biological activity in experiments used ejaculate cryopreserved sperms of three boars (Bank Animal Genetic Resources Institute of Animal Breeding and Genetics nd. a. M.V. Zubets of National Academy of Agrarian Science of Ukraine) and freshly prepared oocytes pigs. Results. In study effect of different concentrations NM (UFS/BSA/NANA) on viability gametes boar. The most active sperms when we add UFS/BSA/NANA in concentration 0.001 %. Adding UFS/BSA/NANA 0.1 % concentration in the cell culture medium has provided formation mature pigs oocytes for significantly higher level, which was 20.2 % more compared to the control and 18.7 % higher, when we add UFS/BSA/NANA in concentration 0.001 %. Conclusions. It is demonstrated the positive effect on defrosted boars sperms with adding UFS/BSA/NANA in concentration 0.001 %, their biological activity increased by 13.3 % compared with control. It is demonstration that add UFS/BSA/NANA in concentration 0.1 % in cell culture medium with pig’s oocytes, level of maturation increased by 20.2 %.Keywords: oocytes, sperms, pigs, cultivation in vitro, ultra-fine silica (UFS).

scholarly journals The Cell Culture Medium Affects Growth, Phenotype Expression and the Response to Selenium Cytotoxicity in A549 and HepG2 Cells

Antioxidants ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 130 ◽  
Author(s):  
Lisa Arodin Selenius ◽  
Marita Wallenberg Lundgren ◽  
Rim Jawad ◽  
Olof Danielsson ◽  
Mikael Björnstedt
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Culture Medium ◽  
Culture Media ◽  
A549 Cells ◽  
Culture Conditions ◽  
Cell Culture Medium ◽  
Cell Culture Media ◽  
Toxic Response ◽  
Selenium Compounds

Selenium compounds influence cell growth and are highly interesting candidate compounds for cancer chemotherapy. Over decades an extensive number of publications have reported highly efficient growth inhibitory effects with a number of suggested mechanisms f especially for redox-active selenium compounds. However, the studies are difficult to compare due to a high degree of variations in half-maximal inhibitor concentration (IC50) dependent on cultivation conditions and methods to assess cell viability. Among other factors, the variability in culture conditions may affect the experimental outcome. To address this, we have compared the maintenance effects of four commonly used cell culture media on two cell lines, A549 and HepG2, evaluated by the toxic response to selenite and seleno-methylselenocysteine, cell growth and redox homeostasis. We found that the composition of the cell culture media greatly affected cell growth and sensitivity to selenium cytotoxicity. We also provided evidence for change of phenotype in A549 cells when maintained under different culture conditions, demonstrated by changes in cytokeratin 18 (CK18) and vimentin expression. In conclusion, our results have shown the importance of defining the cell culture medium used when comparing results from different studies.

scholarly journals Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells

Cells ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 466
Author(s):  
Stefano Panella ◽  
Francesco Muoio ◽  
Valentin Jossen ◽  
Yves Harder ◽  
Regine Eibl-Schindler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
Culture Medium ◽  
Ex Vivo ◽  
Free Cell ◽  
Adipose Stem Cells ◽  
Cell Culture Medium ◽  
Serum Free ◽  
A Cell ◽  
Human Adipose Stem Cells

Adipose tissue is an abundant source of stem cells. However, liposuction cannot yield cell quantities sufficient for direct applications in regenerative medicine. Therefore, the development of GMP-compliant ex vivo expansion protocols is required to ensure the production of a “cell drug” that is safe, reproducible, and cost-effective. Thus, we developed our own basal defined xeno- and serum-free cell culture medium (UrSuppe), specifically formulated to grow human adipose stem cells (hASCs). With this medium, we can directly culture the stromal vascular fraction (SVF) cells in defined cell culture conditions to obtain hASCs. Cells proliferate while remaining undifferentiated, as shown by Flow Cytometry (FACS), Quantitative Reverse Transcription PCR (RT-qPCR) assays, and their secretion products. Using the UrSuppe cell culture medium, maximum cell densities between 0.51 and 0.80 × 105 cells/cm2 (=2.55–4.00 × 105 cells/mL) were obtained. As the expansion of hASCs represents only the first step in a cell therapeutic protocol or further basic research studies, we formulated two chemically defined media to differentiate the expanded hASCs in white or beige/brown adipocytes. These new media could help translate research projects into the clinical application of hASCs and study ex vivo the biology in healthy and dysfunctional states of adipocytes and their precursors. Following the cell culture system developers’ practice and obvious reasons related to the formulas’ patentability, the defined media’s composition will not be disclosed in this study.

Neurons and Neuronal Stem Cells Survive in Glucose-Free Lactate and in High Glucose Cell Culture Medium During Normoxia and Anoxia

2010 ◽  
Vol 35 (10) ◽  
pp. 1635-1642 ◽  
Author(s):  
Sascha Wohnsland ◽  
Heinrich F. Bürgers ◽  
Wolfgang Kuschinsky ◽  
Martin H. Maurer
Keyword(s):  
Stem Cells ◽  
Cell Culture ◽  
High Glucose ◽  
Culture Medium ◽  
Cell Culture Medium ◽  
Neuronal Stem Cells
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