scholarly journals Lactic acid bacteria that activate immune gene expression in Caenorhabditis elegans can antagonise Campylobacter jejuni infection in nematodes, chickens and mice

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Xing Jin ◽  
Yufeng He ◽  
Yonghua Zhou ◽  
Xiaohua Chen ◽  
Yuan-kun Lee ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Caenorhabditis Elegans ◽  
Lactic Acid Bacteria ◽  
Campylobacter Jejuni ◽  
Large Scale ◽  
Protective Mechanism ◽  
Immune Gene ◽  
C Elegans ◽  
Good Ability ◽  
Protection Rate

Abstract Background Campylobacter jejuni is the major micro-bacillary pathogen responsible for human coloenteritis. Lactic acid bacteria (LAB) have been shown to protect against Campylobacter infection. However, LAB with a good ability to inhibit the growth of C. jejuni in vitro are less effective in animals and animal models, and have the disadvantages of high cost, a long cycle, cumbersome operation and insignificant immune response indicators. Caenorhabditis elegans is increasingly used to screen probiotics for their anti-pathogenic properties. However, no research on the use of C. elegans to screen for probiotic candidates antagonistic to C. jejuni has been conducted to date. Results This study established a lifespan model of C. elegans, enabling the preselection of LAB to counter C. jejuni infection. A potential protective mechanism of LAB was identified. Some distinct LAB species offered a high level of protection to C. elegans against C. jejuni. The LAB strains with a high protection rate reduced the load of C. jejuni in C. elegans. The transcription of antibacterial peptide genes, MAPK and Daf-16 signalling pathway-related genes was elevated using the LAB isolates with a high protection rate. The reliability of the lifespan model of C. elegans was verified using mice and chickens infected with C. jejuni. Conclusions The results showed that different LAB had different abilities to protect C. elegans against C. jejuni. C. elegans provides a reliable model for researchers to screen for LAB that are antagonistic to C. jejuni on a large scale.

scholarly journals Lactic acid bacteria that activate immune gene expression in the Caenorhabditis elegans can antagonize the Campylobacter jejuni infection in nematodes, chickens and mice

2020 ◽  
Author(s):  
Xing Jin ◽  
Yufeng He ◽  
Yonghua Zhou ◽  
Xiaohua Chen ◽  
Yuan-kun Lee ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Caenorhabditis Elegans ◽  
Lactic Acid Bacteria ◽  
Life Span ◽  
Campylobacter Jejuni ◽  
Large Scale ◽  
Protective Mechanism ◽  
Immune Gene ◽  
Good Ability ◽  
Protection Rate

Abstract Background Campylobacter jejuni is the major microbacillary pathogen responsible for human coloenteritis. Lactic acid bacteria have been shown to protect against Campylobacter infection. But LAB that showed a good ability to inhibit the growth of C. jejuni in vitro are less effective in antagonising C. jejuni in animals and animal models have the disadvantages of high cost, a long cycle, cumbersome operation and insignificant immune response indicators. Caenorhabditis elegans is increasingly used to screen probiotics for anti-pathogenic property. However, no research on the use of C. elegans to screen for probiotic candidates antagonistic to C. jejuni has been conducted to date. Results This study established a Caenorhabditis elegans life-span model enabling preselection of lactic acid bacteria to counter Campylobacter jejuni infection. A potential protective mechanism of the LAB was identified. 9 strains of distinct LAB species offered the high level of protection for Caenorhabditis elegans. Many of the LAB strains with a high protection rate reduced Campylobacter jejuni load in nematodes. The transcription of antibacterial peptide genes, MAPK and Daf-16 signalling pathway related genes was elevated by the LAB isolates with a high protection rate. The reliability of the life-span model of Caenorhabditis elegans was verified using Campylobacter jejuni -infected mice and chicken model respectively. Conclusions These results showed that LAB vary in their ability to protect Caenorhabditis elegans against Campylobacter jejuni, and Caenorhabditis elegans provides a reliable model enabling researchers to screen for LAB antagonistic to Campylobacter jejuni on a large scale.

scholarly journals Lactic acid bacteria exhibit similar antioxidant capacities in Caenorhabditis elegans- and Campylobacter jejuni-infected mice

RSC Advances ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 3329-3342
Author(s):  
Xing Jin ◽  
Yufeng He ◽  
Zhenmin Liu ◽  
Yonghua Zhou ◽  
Xiaohua Chen ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Caenorhabditis Elegans ◽  
Lactic Acid Bacteria ◽  
Oxidative Damage ◽  
Antioxidant Capacity ◽  
Campylobacter Jejuni ◽  
Analysis Model ◽  
Pathogen Infection ◽  
Antioxidant Capacities

A Caenorhabditis elegans lifespan analysis model exposed to H2O2 and juglone is suitable for identifying lactic acid bacteria with antioxidant capacity that can be used to resist oxidative damage caused by pathogen infection.

scholarly journals Iron Acquisition of Urinary Tract Infection Escherichia coli Involves Pathogenicity in Caenorhabditis elegans

2021 ◽  
Vol 9 (2) ◽  
pp. 310
Author(s):  
Masayuki Hashimoto ◽  
Yi-Fen Ma ◽  
Sin-Tian Wang ◽  
Chang-Shi Chen ◽  
Ching-Hao Teng
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract ◽  
Caenorhabditis Elegans ◽  
Large Scale ◽  
Iron Acquisition ◽  
Infection Model ◽  
High Throughput Analysis ◽  
E Coli ◽  
C Elegans ◽  
Tract Infections

Uropathogenic Escherichia coli (UPEC) is a major bacterial pathogen that causes urinary tract infections (UTIs). The mouse is an available UTI model for studying the pathogenicity; however, Caenorhabditis elegans represents as an alternative surrogate host with the capacity for high-throughput analysis. Then, we established a simple assay for a UPEC infection model with C. elegans for large-scale screening. A total of 133 clinically isolated E. coli strains, which included UTI-associated and fecal isolates, were applied to demonstrate the simple pathogenicity assay. From the screening, several virulence factors (VFs) involved with iron acquisition (chuA, fyuA, and irp2) were significantly associated with high pathogenicity. We then evaluated whether the VFs in UPEC were involved in the pathogenicity. Mutants of E. coli UTI89 with defective iron acquisition systems were applied to a solid killing assay with C. elegans. As a result, the survival rate of C. elegans fed with the mutants significantly increased compared to when fed with the parent strain. The results demonstrated, the simple assay with C. elegans was useful as a UPEC infectious model. To our knowledge, this is the first report of the involvement of iron acquisition in the pathogenicity of UPEC in a C. elegans model.

Frequent Germline Mutations and Somatic Repeat Instability in DNA Mismatch-Repair-Deficient Caenorhabditis elegans

Genetics ◽  
2002 ◽  
Vol 161 (2) ◽  
pp. 651-660
Author(s):  
Marcel Tijsterman ◽  
Joris Pothof ◽  
Ronald H A Plasterk
Keyword(s):  
Caenorhabditis Elegans ◽  
Mismatch Repair ◽  
Large Scale ◽  
Somatic Tissue ◽  
Repeat Instability ◽  
Dna Mismatch ◽  
C Elegans ◽  
Colon Cancers ◽  
Dna Repeat ◽  
Female Germline

Abstract Mismatch-repair-deficient mutants were initially recognized as mutation-prone derivatives of bacteria, and later mismatch repair deficiency was found to predispose humans to colon cancers (HNPCC). We generated mismatch-repair-deficient Caenorhabditis elegans by deleting the msh-6 gene and analyzed the fidelity of transmission of genetic information to subsequent generations. msh-6-defective animals show an elevated level of spontaneous mutants in both the male and female germline; also repeated DNA tracts are unstable. To monitor DNA repeat instability in somatic tissue, we developed a sensitive system, making use of heat-shock promoter-driven lacZ transgenes, but with a repeat that puts this reporter gene out of frame. In genetic msh-6-deficient animals lacZ+ patches are observed as a result of somatic repeat instability. RNA interference by feeding wild-type animals dsRNA homologous to msh-2 or msh-6 also resulted in somatic DNA instability, as well as in germline mutagenesis, indicating that one can use C. elegans as a model system to discover genes involved in maintaining DNA stability by large-scale RNAi screens.

scholarly journals Review on the Biological Detoxification of Mycotoxins Using Lactic Acid Bacteria to Enhance the Sustainability of Foods Supply

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2655 ◽  
Author(s):  
Belal J. Muhialdin ◽  
Nazamid Saari ◽  
Anis Shobirin Meor Hussin
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Large Scale ◽  
Negative Impact ◽  
Cost Effective ◽  
Economic Loss ◽  
Current Review ◽  
Good Potential ◽  
Biological Detoxification ◽  
Safe Food

The challenges to fulfill the demand for a safe food supply are dramatically increasing. Mycotoxins produced by certain fungi cause great economic loss and negative impact on the sustainability of food supplies. Moreover, the occurrence of mycotoxins at high levels in foods poses a high health threat for the consumers. Biological detoxification has exhibited a high potential to detoxify foodstuffs on a cost-effective and large scale. Lactic acid bacteria showed a good potential as an alternative strategy for the elimination of mycotoxins. The current review describes the health and economic impacts associated with mycotoxin contamination in foodstuffs. Moreover, this review highlights the biological detoxification of common food mycotoxins by lactic acid bacteria.

scholarly journals Large-scale genome-wide analysis links lactic acid bacteria from food with the gut microbiome

2020 ◽  
Vol 11 (1) ◽  
Author(s):  
Edoardo Pasolli ◽  
Francesca De Filippis ◽  
Italia E. Mauriello ◽  
Fabio Cumbo ◽  
Aaron M. Walsh ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Gut Microbiome ◽  
Large Scale ◽  
Streptococcus Thermophilus ◽  
Fermented Food ◽  
Fermented Foods ◽  
Genome Wide Analysis ◽  
Genome Wide ◽  
Stool Samples

Abstract Lactic acid bacteria (LAB) are fundamental in the production of fermented foods and several strains are regarded as probiotics. Large quantities of live LAB are consumed within fermented foods, but it is not yet known to what extent the LAB we ingest become members of the gut microbiome. By analysis of 9445 metagenomes from human samples, we demonstrate that the prevalence and abundance of LAB species in stool samples is generally low and linked to age, lifestyle, and geography, with Streptococcus thermophilus and Lactococcus lactis being most prevalent. Moreover, we identify genome-based differences between food and gut microbes by considering 666 metagenome-assembled genomes (MAGs) newly reconstructed from fermented food microbiomes along with 154,723 human MAGs and 193,078 reference genomes. Our large-scale genome-wide analysis demonstrates that closely related LAB strains occur in both food and gut environments and provides unprecedented evidence that fermented foods can be indeed regarded as a possible source of LAB for the gut microbiome.

scholarly journals Probiotics and Postbiotics as Substitutes of Antibiotics in Farm Animals: A Review

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3431
Author(s):  
Daria Zamojska ◽  
Adriana Nowak ◽  
Ireneusz Nowak ◽  
Ewa Macierzyńska-Piotrowska
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Large Scale ◽  
Culture Media ◽  
Body Weight Gain ◽  
Antimicrobial Effect ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Farm Animals ◽  
The European Union

Since 2006, the use of growth-promoting antibiotics has been banned throughout the European Union. To meet the expectations of livestock farmers, various studies have been carried out with the use of lactic acid bacteria. Scientists are trying to obtain the antimicrobial effect against the most common pathogens in large-scale farms. Supplementing the diet of broilers with probiotics (live, nonpathogenic microorganisms) stabilized the intestinal microbiota, which improved the results of body weight gain (BWG) and feed intake (FI). The positive effect of probiotics based on lactic acid bacteria has been shown to prevent the occurrence of diarrhea during piglet weaning. The antagonistic activity of postbiotics (inanimate bacteria, cell components, or post-fermentation by-products) from post-culture media after lactobacilli cultures has been proven on Staphylococcus aureus—the pathogen most often responsible for causing mastitis among dairy cows. The article aims to present the latest research examining the antagonistic effect of lactic acid bacteria on the most common pathogens in broilers, piglets, pigs, and cow farms.

scholarly journals Caenorhabditis elegans, a Host to Investigate the Probiotic Properties of Beneficial Microorganisms

2020 ◽  
Vol 7 ◽  
Author(s):  
Cyril Poupet ◽  
Christophe Chassard ◽  
Adrien Nivoliez ◽  
Stéphanie Bornes
Keyword(s):  
Caenorhabditis Elegans ◽  
High Throughput Screening ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Probiotic Properties ◽  
C Elegans ◽  
Probiotic Potential ◽  
Challenges And Opportunities ◽  
Future Challenges

Caenorhabditis elegans, a non-parasitic nematode emerges as a relevant and powerful candidate as an in vivo model for microorganisms-microorganisms and microorganisms-host interactions studies. Experiments have demonstrated the probiotic potential of bacteria since they can provide to the worm a longer lifespan, an increased resistance to pathogens and to oxidative or heat stresses. Probiotics are used to prevent or treat microbiota dysbiosis and associated pathologies but the molecular mechanisms underlying their capacities are still unknown. Beyond safety and healthy aspects of probiotics, C. elegans represents a powerful way to design large-scale studies to explore transkingdom interactions and to solve questioning about the molecular aspect of these interactions. Future challenges and opportunities would be to validate C. elegans as an in vivo tool for high-throughput screening of microorganisms for their potential probiotic use on human health and to enlarge the panels of microorganisms studied as well as the human diseases investigated.

Sign in / Sign up

Export Citation Format

Share Document