scholarly journals Isolation and functional characterization of a glucose-6-phosphate/phosphate translocator (IbG6PPT1) from sweet potato (Ipomoea batatas (L.) Lam.)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhengdan Wu ◽  
Zhiqian Wang ◽  
Kai Zhang

AbstractSweet potato (Ipomoea batatas (L.) Lam.) is a good source of carbohydrates, an excellent raw material for starch-based industries, and a strong candidate for biofuel production due to its high starch content. However, the molecular basis of starch biosynthesis and accumulation in sweet potato is still insufficiently understood. Glucose-6-phosphate/phosphate translocators (GPTs) mediate the import of glucose-6-phosphate (Glc6P) into plastids for starch synthesis. Here, we report the isolation of a GPT-encoding gene, IbG6PPT1, from sweet potato and the identification of two additional IbG6PPT1 gene copies in the sweet potato genome. IbG6PPT1 encodes a chloroplast membrane–localized GPT belonging to the GPT1 group and highly expressed in storage root of sweet potato. Heterologous expression of IbG6PPT1 resulted in increased starch content in the leaves, root tips, and seeds and soluble sugar in seeds of Arabidopsis thaliana, but a reduction in soluble sugar in the leaves. These findings suggested that IbG6PPT1 might play a critical role in the distribution of carbon sources in source and sink and the accumulation of carbohydrates in storage tissues and would be a good candidate gene for controlling critical starch properties in sweet potato.

2021 ◽  
Author(s):  
Zhengdan Wu ◽  
Zhiqian Wang ◽  
Kai Zhang

Abstract Sweet potato (Ipomoea batatas (L.) Lam.) is a good source of carbohydrates, an excellent raw material for starch-based industries, and a strong candidate for biofuel production due to its high starch content. However, the molecular basis of starch biosynthesis and accumulation in sweet potato is still insufficiently understood. Glucose-6-phosphate/phosphate translocators (GPTs) mediate the import of glucose-6-phosphate (Glc6P) into plastids for starch synthesis. Here, we report the isolation of a GPT-encoding gene, IbG6PPT1, from sweet potato and the identification of two additional IbG6PPT1 gene copies in the sweet potato genome. IbG6PPT1 encodes a chloroplast membrane–localized GPT belonging to the GPT1 group and highly expressed in storage root of sweet potato. Heterologous expression of IbG6PPT1 resulted in increased starch content in the leaves, root tips, and seeds and soluble sugar in seeds of Arabidopsis thaliana, but a reduction in soluble sugar in the leaves. These findings suggested that IbG6PPT1 might play a critical role in carbohydrate accumulation in storage tissues and would be a good candidate gene for controlling critical starch properties in sweet potato.


Nativa ◽  
2018 ◽  
Vol 6 (4) ◽  
pp. 352
Author(s):  
Adriano Mendes Lourenço ◽  
Aline Torquato Tavares ◽  
Tiago Alves Ferreira ◽  
Danilo Alves da Silva Porto Lopes ◽  
João Victor Gonçalves Carline ◽  
...  

A batata-doce (Ipomoea batatas (L.) Lam.) tem sido reportada como uma das espécies de planta com grande capacidade de converter biomassa em matéria prima para produção de etanol. O objetivo do trabalho foi avaliar o potencial de clones de batata-doce para produção de etanol. Foram avaliados 60 clones de batata-doce para produtividade de raízes, teor de amido nas raízes, produtividade de amido, coloração da casca e da polpa e o rendimento de etanol. O clone BDTO#122,32 e as cultivares Ana Clara e Carolina Vitória com média de 46,77; 42,75 e 41,25 t ha-¹, respectivamente, foram os que mais conseguiram acumular biomassa na forma de raiz. Os clones que apresentam as maiores médias de produtividade de amido por hectare foram BDTO#144.22 e BDTO#100.23, com valores de 15,46 e 14,16% t ha-1, com rendimentos de etanol de 8,33 e 7,63 m³ ha-¹. Os clones BDTO#144.22 e BDTO#100.23 apresentaram as maiores médias de produtividade de amido por hectare e rendimento de etanol, sendo, portanto, os mais promissores para a produção de etanol.Palavras-chave: Ipomoea batatas (L.) Lam, melhoramento genético, seleção, biocombustível. POTENTIAL OF EXPERIMENTAL CLONES OF SWEET POTATO FOR ETHANOL PRODUCTION ABSTRACT:Sweet potato (Ipomoea batatas (L.) Lam.) Has been reported as one of the plant species with great ability to convert biomass into feedstock for ethanol production. The objective of this work was to evaluate the potential of sweet potato clones for ethanol production. Twenty-six sweet potato clones were evaluated for root productivity, root starch content, starch yield, bark and pulp color, and ethanol yield. Clone BDTO # 122.32 and cultivars Ana Clara and Carolina Vitória averaging 46.77; 42.75 and 41.25 t ha-1, respectively, were the ones that were able to accumulate biomass in the root form. The clones presenting the highest starch productivity per hectare were BDTO # 144.22 and BDTO # 100.23, with values of 15.46 and 14.16% t ha-1, with ethanol yields of 8.33 and 7.63 m³ ha-¹. The clones BDTO # 144.22 and BDTO # 100.23 showed the highest averages of starch productivity per hectare and yield of ethanol, thus being the most promising for the production of ethanol.Keywords: Ipomoea potatoes (L.) Lam, breeding, selection, biofuel.


2018 ◽  
Author(s):  
Musdalipah

Body scrub is cosmetic preparation used for skin care. One of the natural ingredients that can be used as raw material body scrub is purple sweet potato. Purple sweet potato contains vitamins C, E, A, carotene acids, phenols, polyphenols, and flavonoids are good for the skin. Others,polysaccharides from purple sweet potato are potential natural antioxidant.This study aims to formulate juice of purple sweet potato (Ipomoea batatas L.) varieties Ayamurasaki that formula A of 40%, formula B of 45% B, and formula C of 50%. This type of research is experimental. Testing includes homogenity test , pH test, organoleptic test, irritation test, and emulsion type test. Juice obtained by sampling grated until smooth, after that squeezed flannel cloth to obtain pollen purple sweet potato. The results showed preparations are designed to meet the physical evaluation semisolid dosage in the texture, a distinctive smell, color brown, homogeneous, oil in water emulsion type, pH ranges from 6.1 to 6.2, and does not irritate. The good body scub used is 45% of formula B


2019 ◽  
Vol 35 (6) ◽  
Author(s):  
Daiane da Silva Nóbrega ◽  
José Ricardo Peixoto ◽  
Michelle Souza Vilela ◽  
Anne Kelly da Silva Nóbrega ◽  
Elaine Caetano Santos ◽  
...  

Sweet potato (Ipomoea batatas) is a rustic horticultural crop with high production potential. However, the crop is susceptible to many pests and diseases. The objective of this study was to evaluate 10 genotypes of sweet potato regarding their yield and resistance to soil insects, under Brazilian cerrado soil conditions. Genotypes were selected from the Sweet Potato Germplasm Bank of Embrapa Hortaliças. The experiment was conducted at Água Limpa Farm, belonging to University of Brasilia (UnB), and consisted of a randomized block design, with 10 treatments (genotypes), 10 plants per plot, and four replications. The following traits were analyzed: number of perforations per root, incidence of roots injured by insects, plant resistance degree, root shape, total and marketable root yields, root peel color, root pulp color, pulp total soluble solids, pulp titratable acidity, pulp TSS/TA ratio, pulp moisture, and pulp starch yield. Genotype CNPH 53 (26.78 t ha-1) presented total root yield greater than the commercial variety Brazlândia Rosada (17.54 t ha-1). Genotype Santa Sofia (11.77 t ha-1) and Brazlândia (13.5 t ha-1) had similar marketable root yields. CNPH 53 showed the best agronomic performance, exhibiting moderate susceptibility to soil insects and root shape meeting the market standards. It also had low pulp TA (2.53%); high pulp TSS (12.25 °Brix) and pulp TSS/AT ratio (4.24); pulp moisture content close to 70%; and the highest pulp starch content (11.98%). The traits number of perforations per root, root shape, and pulp TA presented heritability values close to 70%. Marketable root yield, pulp moisture, and pulp starch content demonstrated heritability values greater than 90% and CVG/CVE greater than 1.


2020 ◽  
Vol 4 (1) ◽  
pp. 14-23
Author(s):  
Isana Supiah Yosephine Louise

AbstrakInulin merupakan bahan dasar obat yang banyak terkandung dalam umbi-umbian lokal, seperti ubi kayu/singkong (Manihot esculenta), uwi (Dioscorea spp.), ubi jalar (Ipomoea batatas), dan gembili (Dioscorea esculenta L.), yang secara luas belum diupayakan untuk diproduksi secara besar-besaran. Umbi-umbian lokal umumnya hanya dijual sebagai bahan mentah yang harganya relatif murah dan tidak dapat disimpan dalam waktu yang relatif lama. Umumnya dikonsumsi dengan cara direbus, digoreng atau diolah menjadi makanan tradisional, belum diproses menjadi produk yang lebih bermanfaat, memiliki nilai jual relatif tinggi, dan waktu penyimpanan yang relatif lebih lama. Pada kegiatan pemberdayaan masyarakat pedesaan ini telah dilakukan penyuluhan, pemberian keterampilan, dan pendampingan masyarakat dusun Krajan Wedomartani Ngemplak Sleman untuk secara mandiri mampu mengolah umbi-umbian lokal menjadi produk tepung fermentasi, tepung non fermentasi, dan ekstrak inulin. Pada kegiatan ini bahan baku yang digunakan adalah umbi singkong, uwi ungu, dan ubi jalar ungu. Proses fermentasi umbi dilakukan menggunakan jamur monascus angka atau monascus purpureus. Ekstrak inulin dilakukan dengan menggunakan pelarut alkohol. Produk tepung fermentasi dengan bahan baku umbi singkong, uwi ungu, dan ubi jalar ungu masing-masing memiliki randemen sebesar 52%, 43%, dan 40%; sedangkan ekstrak inulin memiliki randemen sesesar 7,47%, hanya saja produk inulin belum optimum bila ditinjau dari sisi warna senyawa, masih perlu dilakukan proses lanjutan untuk memperoleh produk inulin yang sesuai standar pasar. Hasi angket menunjukkan bahwa sebagian besar peserta menyatakan kegiatan seperti ini sangat bermanfaat, dan secara umum penyajian materi oleh tim pengabdi dapat diterima dengan mudah dan jelas oleh peserta. Sebagian besar peserta menyatakan bahwa metoda penyampaian materi sesuai, artinya, secara umum metoda penyampaian materi dalam kegiatan ini dapat diterima dengan mudah oleh masyarakat sasaran. Sebagian besar peserta menyatakan bahwa kegiatan seperti ini sangat membantu membuka wawasan masyarakat desa untuk dapat mengolah umbi-umbian lokal, yang semula hanya dikonsumsi dengan direbus atau digoreng atau dijual mentah dengan harga realtif murah menjadi produk yang lebih bermanfaat, memiliki nilai jual relatif tinggi, dan memiliki daya simpan relatif lebih lama. Kata kunci: inulin, monascus angka, umbi-umbian lokal, membuka wawasan, masyarakat desa The Production of Tubers-Based Inulin as The Basic Ingredients of Medicine AbstractInulin is a raw material of medicine which is contained in local tubers, such as cassava (Manihotes culenta), Dioscorea spp., sweet potato (Ipomoea batatas L.), and Dioscorea esculenta L., which is not widely strived in mass production. Local tubers commonly sold as food raw material which is relatively cheap and cannot be stored for a long time. Generally, the tubers consumed by boiling, fried or processed to traditional food. It has not processed to be more valuable product, has relatively high price, and has relatively longer the storage time. In the empowerment activity of rural community, it has been conducted counseling, providing soft skill, and community assistance in Krajan, Wedomartani, Ngemplak, Sleman to independently be able producing local tubers became fermented flour, unfermented flour, and inulin extract product. In this activity, the raw material were cassava, Dioscorea alata L. syn. D. atropurpurea Roxb., and purple sweet potato (Ipomoea batatas var Ayumurasaki). Tubers fermentation process was conducted using monascus angka or monascus purpureus mold. Inulin extract was produced using alcohol solvent. Fermented flour product from cassava, Dioscorea alata L. syn. D. atropurpurea Roxb., and purple sweet potato have yield as respectively 52%, 43%, and 40%; meanwhile inulin extract has yield as 7.47%, although inulin product had not reach optimum point regarding on the color of compound. It still needs further process to gain standardized commercially inulin product. Questioners showed that mostly of the attendances stated the activity very useful, and generally the presentation from empowerment team can be delivered easily and clearly to the communities.  Mostly of the attendances stated that the method of presentation is suitable, which means generally the presentation method in this activity can be accepted easily by the communities. Mostly of the attendances stated that the activity is very helpful for them to gain the knowledge of local tubers processing, which was originally consumed only with boiled or fried or sold uncooked with the relatively cheap price to be more valuable product, has relatively high price, and has relatively longer the storage time  Key words: inulin, monascus angka, local tubers, empowerment, rural community


2018 ◽  
Vol 6 (2) ◽  
pp. 77
Author(s):  
RTM Sutamihardja ◽  
Nia Yuliani ◽  
Hana Laelasari ◽  
Devy Susanty

Acid Hydrolysis on The Starch Flour of white sweet potato (Ipomoea batatas L.) in Making of Liquid SugarNational sugar needs for both direct consumption and for industrial needs will continue to increase as the population increases. According to Dirjenbun, in 2014 the national sugar demand reaches 5.7 million tons. Consisting of 2.8 million tons of white crystalline sugar for direct community consumption and 2.9 million tons of refined crystal sugar to meet industrial needs. White sweet potato can be used as raw material for making liquid glucose through hydrolysis process with acid (HCl). The preparation of liquid glucose consists of two stages: gelatinization stage and hydrolysis stage. Optimum hydrolysis was determined by variations in HCl concentration of 0.25; 0.5; and 0.75 N and time variations of 30, 60, and 90 minutes. The yield of sweet potato starch was 28.82% and the highest yield of hydrolysis result of white sweet potato starch was 94.07% at acid concentration 0.75 N with hydrolysis time 90 minutes.Keywords: white sweet potato, Flour of white sweet potato, liquid sugar, acid hydrolysisABSTRAKKebutuhan gula nasional baik untuk konsumsi langsung maupun untuk kebutuhan industri akan terus meningkat sejalan dengan meningkatnya jumlah penduduk. Menurut Dirjenbun, pada tahun 2014 kebutuhan gula nasional mencapai 5,7 juta ton yang terdiri dari 2,8 juta ton gula kristal putih untuk konsumsi masyarakat langsung dan 2,9 juta ton gula kristal rafinasi untuk memenuhi kebutuhan industri. Ubi jalar putih dapat dijadikan bahan baku pembuatan glukosa cair melalui proses hidrolisis dengan asam (HCl). Pembuatan glukosa cair terdiri dari dua tahap yaitu tahap gelatinisasi dan tahap hidrolisis. Hidrolisis optimum ditentukan dengan variasi konsentrasi HCl yaitu 0,25; 0,5; dan 0,75 N dan variasi waktu 30, 60, dan 90 menit. Rendemen pati ubi jalar didapatkan sebesar 28,82% dan rendemen glukosa tertinggi hasil hidrolisis pati ubi jalar putih sebesar 94,07% pada konsentrasi asam 0,75 N dengan waktu hidrolisis 90 menit.Kata kunci: Ubi jalar putih, Tepung Pati Ubi Jalar Putih, Gula cair, hidrolisis asam


2019 ◽  
Vol 20 (3) ◽  
pp. 483 ◽  
Author(s):  
Kangyong Zha ◽  
Haoxun Xie ◽  
Min Ge ◽  
Zimeng Wang ◽  
Yu Wang ◽  
...  

As major component in cereals grains, starch has been one of the most important carbohydrate consumed by a majority of world’s population. However, the molecular mechanism for regulation of biosynthesis of starch remains elusive. In the present study, ZmES22, encoding a MADS-type transcription factor, was modestly characterized from maize inbred line B73. ZmES22 exhibited high expression level in endosperm at 10 days after pollination (DAP) and peaked in endosperm at 20 DAP, indicating that ZmES22 was preferentially expressed in maize endosperm during active starch synthesis. Transient expression of ZmES22 in tobacco leaf revealed that ZmES22 protein located in nucleus. No transactivation activity could be detected for ZmES22 protein via yeast one-hybrid assay. Transformation of overexpressing plasmid 35S::ZmES22 into rice remarkedly reduced 1000-grain weight as well as the total starch content, while the soluble sugar was significantly higher in transgenic rice lines. Moreover, overexpressing ZmES22 reduced fractions of long branched starch. Scanning electron microscopy images of transverse sections of rice grains revealed that altered expression of ZmES22 also changed the morphology of starch granule from densely packed, polyhedral starch granules into loosely packed, spherical granules with larger spaces. Furthermore, RNA-seq results indicated that overexpressing ZmES22 could significantly influence mRNA expression levels of numerous key regulatory genes in starch synthesis pathway. Y1H assay illustrated that ZmES22 protein could bind to the promoter region of OsGIF1 and downregulate its mRNA expression during rice grain filling stages. These findings suggest that ZmES22 was a novel regulator during starch synthesis process in rice endosperm.


2017 ◽  
Vol 8 ◽  
Author(s):  
Suravoot Yooyongwech ◽  
Thapanee Samphumphuang ◽  
Rujira Tisarum ◽  
Cattarin Theerawitaya ◽  
Suriyan Cha-um

Sign in / Sign up

Export Citation Format

Share Document