The protective effect of crocin on cisplatin-induced testicular impairment in rats

Abstract Background Side effects of cisplatin (CIS) such as testicular toxicity restrict its clinical use. Instead, evidence indicates that crocin (CR) has synergistic anti-cancer potential with CIS and exhibited beneficial effects on CIS-induced hepatorenal damage. The aim of this study was to investigate the protective potential of CR against CIS-induced testicular toxicity in rats. Methods Fifty adult male Wistar rats randomly assigned to five equal groups including control, CIS, and CIS plus CR at doses of 6.25 mg/kg (CIS + CR6.25), 25 mg/kg (CIS + CR25), and 100 mg/kg (CIS + CR100). CIS and CIS + CR groups received a single intraperitoneally (i.p.) injection of CIS (7 mg/kg). CR (6.25–100 mg/kg i.p.) injections were started three days before the CIS injection and continued once a day for up to 13 days. On the 14th day, all animals were sacrificed and their blood samples and testes were removed for biochemical and histological analyses. Results Compared to the control group, CIS significantly decreased relative testis weight (0.28 vs. 0.39, p < 0.001), testosterone level (0.3 vs. 2.31 ng/mL, p < 0.001), germinal layer area (25,886 vs. 35,320 µm2, p < 0.001), superoxide dismutase (SOD) (0.9 vs.1.73 U/mg, p < 0.001) and increased testicular lipid peroxidation (3.05 vs. 15.35 nmol/mg, p < 0.001). CR at 25 mg/kg ameliorated testicular lipid peroxidation and enhanced SOD activity compared to CIS group (p < 0.05). Besides, CR treatment at the maximum dose (100 mg/kg) resulted in reversing CIS effects on testis weight, testosterone level, SOD, lipid peroxidation, and germinal layer area. Conclusions These findings demonstrated that CR co-treatment could prevent CIS-induced testicular toxicity in rats.

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Effect of 12 Months of Exercise with Stachys Lavandulifolia Consumption on anxiety, Metabolic Syndrome Profiles and Antioxidant Defense and Lipid Peroxidation in Women with Syndrome Metabolic

10.21203/rs.3.rs-129277/v1 ◽

2020 ◽

Author(s):

Ali osali ◽

Alireza Rostami

Keyword(s):

Metabolic Syndrome ◽

Lipid Peroxidation ◽

Aerobic Exercise ◽

Antioxidant Defense ◽

Training Group ◽

Serum Levels ◽

Control Group ◽

Negative Effects ◽

Beneficial Effects ◽

Before And After

Abstract BackgroundThe purpose of this study was to investigate the effect of 12 months of aerobic exercise combining stachys lavandulifolia (S. lavandulifolia) consumption on anxiety, Metabolic Syndrome profiles and antioxidant defense (Glutathione) and lipid peroxidation (Malondialdehyde) in 50-65 years old women with syndrome metabolic.Methods48 women with syndrome Metabolic were randomly divided into four groups: exercise (n=12), exercise+S. lavandulifolia (n=12), S. lavandulifolia (n=12) and control group (n=12). S. lavandulifolia groups consumed 3 g aerial parts of S. lavandulifolia daily. Training groups performed an exercise protocol of aerobic exercise for 12 months (three sessions per week). Blood samples were obtained before and after training period for antioxidant indicators and lipid degradation measurement. Also, Beck anxiety questionnaire used for evaluating levels of anxiety. T-test and one-way analysis of variance were used for the evaluation of within-group and between-group differences, respectively.ResultsA significant increase was observed in serum levels of Malondialdehyde (P =0.004), Catalase indexes (Pvalue= 0.01), and Glutathione (P=0.001) in the training group and S. lavandulifolia groups after 12 months. Body weight, BMI, and SBP and Anexiety was decreased significantly greater in exercise +S. lavandulifolia group compared to control, exercise and S. lavandulifolia groups (P=0.001)ConclusionAnxiolytic effect and Anti-Oxidative Stress Activity was seen, so taking S. lavandulifolia along with exercises may have beneficial effects on reinforcement the antioxidant system and prevention of anxiety and The negative effects of indicators related to cardiovascular disease in women with metabolic syndrome.

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A Subpressor Dose of Angiotensin II Elevates Blood Pressure in a Normotensive Rat Model by Oxidative Stress

Physiological Research ◽

10.33549/physiolres.932738 ◽

2015 ◽

pp. 153-159 ◽

Cited By ~ 1

Author(s):

M. M. GOVENDER ◽

A. NADAR

Keyword(s):

Oxidative Stress ◽

Blood Pressure ◽

Angiotensin Ii ◽

Mrna Expression ◽

Rat Model ◽

Wistar Rat ◽

Control Group ◽

Sod Activity ◽

Male Wistar Rats ◽

Experimental Group

Oxidative stress is an imbalance between free radicals and antioxidants, and is an important etiological factor in the development of hypertension. Recent experimental evidence suggests that subpressor doses of angiotensin II elevate oxidative stress and blood pressure. We aimed to investigate the oxidative stress related mechanism by which a subpressor dose of angiotensin II induces hypertension in a normotensive rat model. Normotensive male Wistar rats were infused with a subpressor dose of angiotensin II for 28 days. The control group was sham operated and infused with saline only. Plasma angiotensin II and H2O2 levels, whole-blood glutathione peroxidase, and AT-1a, Cu/Zn SOD, and p22phox mRNA expression in the aorta was assessed. Systolic and diastolic blood pressures were elevated in the experimental group. There was no change in angiotensin II levels, but a significant increase in AT-1a mRNA expression was found in the experimental group. mRNA expression of p22phox was increased significantly and Cu/Zn SOD decreased significantly in the experimental group. There was no significant change to the H2O2 and GPx levels. Angiotensin II manipulates the free radical-antioxidant balance in the vasculature by selectively increasing O2− production and decreasing SOD activity and causes an oxidative stress induced elevation in blood pressure in the Wistar rat.

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The effect of bee venom and hyperthermia on the lipoperoxidation and antioxidant activity of the blood of tumor-bearing animals

JOURNAL of SIBERIAN MEDICAL SCIENCES ◽

10.31549/2542-1174-2021-3-25-33 ◽

2021 ◽

pp. 25-33

Author(s):

M.A. Shabalin ◽

◽

A.V. Deryugina ◽

V.V. Nazarova ◽

E.A. Gracheva ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Activity ◽

Malignant Tumors ◽

Combined Treatment ◽

Bee Venom ◽

Control Group ◽

Sod Activity ◽

Tumor Bearing ◽

Prolonged Effect ◽

Control Tumor

Introduction. Nowadays, it has been shown that one of the possible ways to increase the effectiveness of the treatment of malignant tumors is the use of combined treatment methods. Aim. To study the intensity of lipid peroxidation (LPO) and the activity of superoxide dismutase (SOD) in hyperthermia against the background of bee venom in the blood of tumor-bearing rats. Materials and methods. The experimental animals were divided into 5 groups: 1st — intact; 2nd — control (tumor-bearing animals (PS-1 tumor strain) with intraperitoneal (IP) administration of saline solution); 3rd, 4th and 5th groups — tumor-bearing animals with IP administration of 0.5 ml of bee venom against the background of hyperthermia 42, 43 and 44°C respectively. The content of diene and triene conjugates, Schiff bases (SB) and SOD activity in the blood of animals was determined. Results. From the 1st day after the end of the experiment, a statistically significant decrease in triene conjugates was recorded in all experimental groups compared to the control group. SB decreased on the 1st–7th day after the end of the experiment with the action of hyperthermia 42°C and bee venom, on the 7th–28th day — with the action of hyperthermia 43°C and bee venom, and on the 28th day — with the action of bee venom and hyperthermia 44°C, which was accompanied by an increase in SOD activity from the 7th day in all experimental groups compared to the control group. Conclusion. Hyperthermia in combination with the action of bee venom causes a decrease in lipid peroxidation products and an increase in antioxidant activity in the blood of tumor-bearing rats. The most effective action, in our opinion, is the use of bee venom against the background of hyperthermia of 43°C, at which a prolonged effect is recorded both with respect to a decrease in the concentration of SB and an increase in the activity of SOD.

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The subchronic effects of 3,4-methylendioxymethamphetamine on oxidative stress in rat brain

Archives of Biological Sciences ◽

10.2298/abs1403075s ◽

2014 ◽

Vol 66 (3) ◽

pp. 1075-1081

Author(s):

Ivan Simic ◽

Violeta Iric-Cupic ◽

Rada Vucic ◽

Marina Petrovic ◽

Violeta Mladenovic ◽

...

Keyword(s):

Oxidative Stress ◽

Lipid Peroxidation ◽

Rat Brain ◽

Wistar Rats ◽

Superoxide Radical ◽

Reduced Glutathione ◽

Oxidative Stress Markers ◽

Sod Activity ◽

Stress Markers ◽

Male Wistar Rats

The aim of the present study was to evaluate the subchronic effects of 3,4-methylenedioxymethamphetamine on several oxidative stress markers: index of lipid peroxidation (ILP), superoxide dismutase (SOD) activity, superoxide radical (O2.-) levels, and reduced glutathione (GSH) levels in the frontal cortex, striatum and hippocampus of the rat. The study included 64 male Wistar rats (200-250g). The animals were treated per os with of 5, 10, or 20 mg/kg of 3,4-methylenedioxymethamphetamine (MDMA) every day for 15 days. The subchronic administration of MDMA resulted in an increase in ILP, SOD and O2.-, and a decrease in GSH, from which we conclude that oxidative stress was induced in rat brain.

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The effect of epidermal growth factor on liver lipid peroxidation and glutathione levels in lobectomized rats

Biochemistry and Cell Biology ◽

10.1139/o92-040 ◽

1992 ◽

Vol 70 (3-4) ◽

pp. 259-262 ◽

Cited By ~ 2

Author(s):

Nahide Gokcora ◽

Sadi Gundogdu ◽

Aysel Aricioglu ◽

Deniz Erbas ◽

Osman Durmus ◽

...

Keyword(s):

Lipid Peroxidation ◽

Growth Factor ◽

Epidermal Growth Factor ◽

Liver Lipid ◽

Lipid Peroxide ◽

Submaxillary Gland ◽

Control Group ◽

Key Factor ◽

Male Wistar Rats ◽

Epidermal Growth

Epidermal growth factor (EGF) is a growth-promoting polypeptide which is found in highest levels in male mice in the submaxillary gland. It may also be a key factor in regeneration of the liver. We performed experiments with 18 male Wistar rats, divided into three groups. Hepatic left lobectomy (%30) was performed on the first group of rats. This group received an intraperitoneal injection of EGF for 7 days. The second group was the control group into which normal saline was injected for 7 days. The third group was sham-operated. On days 5 and 7 tomographic studies of liver were performed. On day 7 EGF levels, lipid peroxidation, and glutathione in liver were measured in all of the rats. While serum EGF levels did not show any significant change, the levels of lipid peroxide were decreased and glutathione was increased. Tomographic measurements indicated that administration of EGF increased the amount of regeneration.Key words: epidermal growth factor, liver lobectomy, lipid peroxide, glutathione, radioimmunoassay.

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Metallothionein-II Inhibits Lipid Peroxidation and Improves Functional Recovery after Transient Brain Ischemia and Reperfusion in Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2014/436429 ◽

2014 ◽

Vol 2014 ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

Araceli Diaz-Ruiz ◽

Patricia Vacio-Adame ◽

Antonio Monroy-Noyola ◽

Marisela Méndez-Armenta ◽

Alma Ortiz-Plata ◽

...

Keyword(s):

Lipid Peroxidation ◽

Frontal Cortex ◽

Neurological Deficit ◽

Neuroprotective Effect ◽

Treated Group ◽

Neurological Deficits ◽

Control Group ◽

Ischemia And Reperfusion ◽

Male Wistar Rats ◽

Neuroprotective Treatment

After transient cerebral ischemia and reperfusion (I/R), damaging mechanisms, such as excitotoxicity and oxidative stress, lead to irreversible neurological deficits. The induction of metallothionein-II (MT-II) protein is an endogenous mechanism after I/R. Our aim was to evaluate the neuroprotective effect of MT-II after I/R in rats. Male Wistar rats were transiently occluded at the middle cerebral artery for 2 h, followed by reperfusion. Rats received either MT (10 μg per rat i.p.) or vehicle after ischemia. Lipid peroxidation (LP) was measured 22 h after reperfusion in frontal cortex and hippocampus; also, neurological deficit was evaluated after ischemia, using the Longa scoring scale. Infarction area was analyzed 72 hours after ischemia. Results showed increased LP in frontal cortex (30.7%) and hippocampus (26.4%), as compared to control group; this effect was fully reversed by MT treatment. Likewise, we also observed a diminished neurological deficit assessed by the Longa scale in those animals treated with MT compared to control group values. The MT-treated group showed a significant (P<0.05) reduction of 39.9% in the infarction area, only at the level of hippocampus, as compared to control group. Results suggest that MT-II may be a novel neuroprotective treatment to prevent ischemia injury.

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Interaction Between Acute Exercise and Carnitine Supplementation on the Expression of Genes Involved in Liver Metabolism in Male Rats

Biointerface Research in Applied Chemistry ◽

10.33263/briac124.43484356 ◽

2021 ◽

Vol 12 (4) ◽

pp. 4348-4356

Keyword(s):

Gene Expression ◽

Acute Exercise ◽

Liver Metabolism ◽

Male Rats ◽

Control Group ◽

Carnitine Supplementation ◽

Serum Factors ◽

Beneficial Effects ◽

Expression Of Genes ◽

Male Wistar Rats

Acute exercise induces rapid and dramatic induction of transcription in the liver. The beneficial effects of carnitine on serum factors and gene expression have been proven. This study examined the interaction between acute exercise and carnitine supplementation on the expression of genes involved in liver metabolism. Thirty-two male Wistar rats were randomly assigned into 4 groups (n = 8): Group 1 control, Group 2 received 200 mg/kg/day LCAR, Group 3 performed acute exercise, and Group 4 received LCAR and performed acute exercise. Gene expression in the liver was evaluated by Real-time PCR. Acute exercise significantly increased PDK4 expression compared to other groups. Also, carnitine administration, performing an acute exercise, and combination of LCAR-Acute significantly increased AMPK and PGC-1a expression compared with the control group. The expression of SREBP-1c and SCD1 was not significantly changed between studies. The combination of acute exercise and carnitine administration increased PGC-1a expression, indicating the importance of carnitine with exercise as a beneficial supplement.

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Protective effects of hesperetin on the quality of sperm, apoptosis, lipid peroxidation, and oxidative stress during the process of cryopreservation: An experimental study

International Journal of Reproductive BioMedicine ◽

10.18502/ijrm.v19i1.8178 ◽

2021 ◽

Author(s):

Jamal Valipour ◽

Sina Mojaverrostami ◽

Beheshteh Abouhamzeh ◽

Masoumeh Abdollahi

Keyword(s):

Reactive Oxygen Species ◽

Lipid Peroxidation ◽

Experimental Study ◽

Reactive Oxygen ◽

Human Sperm ◽

Control Group ◽

Oxygen Species ◽

Protective Effects ◽

Beneficial Effects

Background: Hesperetin is a bioflavonoid compound, largely used in Chinese traditional medicine and found plenty in citrus fruits. Hesperetin has beneficial effects against different diseases. The sperm cryopreservation process is a common method that is used in infertility laboratories. It has been reported that during the cryopreservation process, the quality of sperm is significantly reduced. Objective: To investigate the effect of hesperetin on the quality of human spermatozoa during the cryopreservation process. Materials and Methods: In this experimental study, 22 sperm sample of normozoospermia men who referred to the infertility department of the Shariati Hospital (Tehran, Iran) Between October and November 2019 were collect and divided into three groups as: 1) fresh, 2) control (frozen-thawed group without treatment), and 3) treatment group as frozen-thawed samples supplemented with 20 μM hesperetin. Motility, Viability, morphology, Apoptotic-like changes, intracellular H2O2, intracellular O2−, and lipid peroxidation (LPO) was measured. Results: Hesperetin treatment during the cryopreservation process of human sperm significantly improved the viability, motility, and morphology rates of the spermatozoa after frozen-thawed process in control group (p < 0.01). In addition, it significantly reduced the reactive oxygen species (ROS) level, LPO level and increased the percentage of viable sperm cells with intact plasma membrane (p < 0.01) after frozen-thawed process. Conclusion: Hesperetin can improve the quality of human sperm and also protect human sperm against reactive oxygen species, LPO, and apoptosis during the cryopreservation-thawing process. Key words: Cryopreservation, Hesperetin, Spermatozoa, Reactive oxygen species.

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Pengaruh pemberian ekstrak pasak bumi (Eurycoma longifolia Jack) terhadap kualitas spermatozoa tikus wistar (Rattus norvegicus)

Jurnal e-Biomedik ◽

10.35790/ebm.4.1.2016.10867 ◽

2016 ◽

Vol 4 (1) ◽

Author(s):

Bill C. A. Bogar ◽

Lydia Tendean ◽

Grace L. A. Turalaki

Keyword(s):

Rattus Norvegicus ◽

Control Group ◽

Adult Rats ◽

Beneficial Effects ◽

Eurycoma Longifolia ◽

Male Wistar Rats ◽

Group 3 ◽

Eurycoma Longifolia Jack ◽

Group 2 ◽

Group 1

Abstract: Infertility is an inability of couples to reproduce after carrying out sexual intercourses for at least a year without using any contraceptives. There are 10-15% couples worldwide who experience infertility problems and almost one half of them is on men. Spermatozoa qualities including concentration, motility, and morphology are used as an indicator for men’s fertility. The utilization of herbal medicine (i.e. Eurycoma longifolia Jack extracts) is now being an alternative way to improve fertility among the community. This study was carried out to find the effects of Eurycoma longifolia Jack on spermatozoa qualities. This experimental study was conducted to nine male wistar rats (Rattus norvegicus) weighing from 200-250 grams, aging from 12-15 months. These nine adult rats were divided into 3 groups of 3 rats each. 400 and 600 mg/kg/day of Eurycoma longifolia Jack extracts were orally administrated to group 1 and 2 respectively, while group 3 were treated as control group. After 50 days, the animals of group 1, 2 and 3 were sacrificed. As a result, there is an improvement in qualities of spermatozoa which are statistically significant (p<0,05) in group 2. Thus, provide the beneficial effects of 600mg/kg Eurycoma longifolia Jack extracts on the qualities of spermatozoa.Keywords: pasak bumi, spermatozoaAbstrak: Infertilitas adalah ketidakmampuan pasangan untuk dapat hamil setelah satu tahun berhubungan intim tanpa menggunakan kontrasepsi. Ada sekitar 10 – 15 % pasangan mengalami masalah infertilitas dan hampir setengahnya masalah ada pada pria. Kualitas spermatozoa meliputi konsentrasi, motilitas dan morfologi spermatozoa merupakan salah satu indikator fertilitas pada pria. Penggunaan tanaman herbal atau yang lebih dikenal jamu telah menjadi pengobatan alternatif di masyarakat. Tanaman herbal yang dimaksud adalah pasak bumi. Penelitian ini bertujuan untuk mengetahui efek dari esktrak pasak bumi (Eurycoma longifolia Jack) terhadap kualitas spermatozoa. Penelitian ini menggunakan 9 ekor tikus wistar jantan (Rattus norvegicus) dengan berat badan 200-250 g berumur 12-15 bulan. Sembilan ekor wistar dibagi menjadi tiga kelompok, dan satu kelompok terdiri dari tiga ekor tikus wistar. Penelitian ini dilaksankan selama 50 hari. Esktrak pasak bumi (Eurycoma longifolia Jack) diberikan dengan dosis 400mg/kgBB per hari pada perlakuan 1 dan dosis 600 mg/kgBB per hari pada perlakuan 2. Setelah 50 hari hewan coba pada kelompok kontrol, perlakuan 1 dan perlakuan 2 diterminasi. Hasil penelitian memperlihatkan terjadi peningkatan kualitas spermatozoa yang signifikan secara statistik (p<0,05) pada perlakuan 2 terhadap kelompok kontrol. Hasil tersebut menunjukan bahwa Esktrak pasak bumi (Eurycoma longifolia Jack) pada dosis 600 mg/kgBB dapat meningkatkan kualitas spermatozoa.Kata kunci: pasak bumi, spermatozoa

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The Effect of Captopril and Losartan on Tacrolimus-induced Testicular Toxicity in Rats

Shiraz E-Medical Journal ◽

10.5812/semj.106199 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Seyedeh Mahsa Poormoosavi ◽

Behnam Ghorbanzadeh ◽

Mohammad Amin Behmanesh ◽

Kooshan Ghobadi ◽

Reza Amirgholami

Keyword(s):

Renin Angiotensin System ◽

Serum Levels ◽

Immunosuppressive Drug ◽

Angiotensin Receptors ◽

Seminiferous Tubules ◽

Control Group ◽

Testicular Toxicity ◽

Angiotensin System ◽

Blocking Effects ◽

Male Wistar Rats

Background: Tacrolimus is an immunosuppressive drug broadly used to avoid rejection of tissue transplantation. Some studies suggest that tacrolimus has destructive effects on the male reproductive system due to activation of the renin-angiotensin system (RAS) and inflammation. Objectives: The present study aimed at determining the toxic effects of tacrolimus on testis and evaluating the blocking effects of captopril and losartan on angiotensin-converting enzyme (ACE) and angiotensin receptors, respectively, in rats. Methods: The present experimental study was conducted on 36 adult male Wistar rats (weighing 200 ± 20 g) randomly divided into six groups and treated based on the experimental design for 30 days. At the end of the intervention, the rats were anesthetized and their blood samples were obtained to measure the serum levels of testosterone and ACE2 enzyme. Also, both testes were removed for histopathological examinations after scarifying. Results: Tacrolimus decreased testosterone and ACE2 levels significantly. The ACE2 levels were lower in the groups treated with a combination of tacrolimus and losartan or captopril compared to the control group while testosterone level significantly increased in the groups receiving a combination of tacrolimus and captopril. The epithelial thickness of seminiferous tubules and the number of primary spermatocytes significantly decreased in the rats only receiving tacrolimus compared to the groups that received captopril and losartan, along with tacrolimus. Conclusions: According to the findings, the administration of captopril or losartan, along with tacrolimus, can decrease its testicular toxicity. Captopril and losartan act through blocking RAS but cannot improve ACE2 level; therefore, the recovery of testicle tissue might not be attributed to the ACE2 approach but to the anti-inflammatory effect.

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