scholarly journals The promising use of nano-molecular imprinted templates for improved SARS-CoV-2 detection, drug delivery and research

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Alaa F. Nahhas ◽  
Thomas J. Webster
Keyword(s):  
Drug Delivery ◽  
Binding Sites ◽  
Solid Phase ◽  
Soft Materials ◽  
Disease Diagnosis ◽  
Antigenic Determinants ◽  
Extraction Separation ◽  
Therapeutic Tools ◽  
Proteins And Peptides ◽  
Complementary Binding

AbstractMolecular imprinting (MI) is a technique that creates a template of a molecule for improving complementary binding sites in terms of size and shape to a peptide, protein, bacteria, mammalian cell, or virus on soft materials (such as polymers, hydrogels, or self-assembled materials). MI has been widely investigated for over 90 years in various industries but is now focused on improved tissue engineering, regenerative medicine, drug delivery, sensors, diagnostics, therapeutics and other medical applications. Molecular targets that have been studied so far in MI include those for the major antigenic determinants of microorganisms (like bacteria or viruses) leading to innovations in disease diagnosis via solid-phase extraction separation and biomimetic sensors. As such, although not widely investigated yet, MI demonstrates much promise for improving the detection of and treatment for the current Coronavirus Disease of 2019 (COVID-2019) pandemic as well as future pandemics. In this manner, this review will introduce the numerous applications of MI polymers, particularly using proteins and peptides, and how these MI polymers can be used as improved diagnostic and therapeutic tools for COVID-19. Graphic Abstract

Four Sets of Complementary Binding Sites Involved in Fibrin Polymerization.

1979 ◽  
Author(s):  
Stephanie A. Olexa ◽  
Andrei Z. Budzynski
Keyword(s):  
Binding Sites ◽  
Solid Phase ◽  
Degradation Products ◽  
Monomer Molecule ◽  
Terminal Domain ◽  
Fibrin Monomer ◽  
D Region ◽  
Single Fragment ◽  
Phase Systems ◽  
Complementary Binding

Activation of fibrinogen by thrombin releases fibrinopeptides A and B, followed by the formation of an ordered fibrin polymer, probably due to the association of complementary binding sites. The present investigation focused on defining areas of fibrin monomer molecule which are active during polymerization by identifying binding regions on plasmic degradation products of fibrinogen or fibrin. Studies were done in soluble and in solid phase systems. In the first, species were mixed in solution and monitored for complex formation on Tris-glycine gels; in the latter, affinity chromatography on insolublized fibrinogen, fibrin monomer or cross linked fibrin was done. Thrombin-treated fibrinogen, Fragments X, Y, or NDSK bound to Fragment DD in solution as well as to all three insolublized species. Therefore one type of polymerization site is present and available on the Fragment D region of fibrinogen. The complementary site is a thrombin activated region on th e NH2-terminal domain of fibrinogen. Fragments E1 and E2 from crosslinked fibrin complexed with Fragment DD but not with fibrinogen, Fragments X, Y, or D. Fragment E1 and E2 also bound to insolublized crosslinked fibrin but not to insolublized fibrinogen or fiorin monomer. The data indicate that Fragment DD contains a binding site generated in the aligned Fragment D moieties which is inoperative in a single Fragment D. This site is complementary to the fourth site, a thrombin-activated region on the NH2-terminal domain of fibrinogen localized to Fragments E1 and E2. It is concluded that the first two binding sites are active on fibrin monomer molecules but the other two sites become operative only after the formation of fibrin oligomers.

Lipospheres: Emerging Carriers in the Delivery of Proteins and Peptides

1970 ◽  
Vol 1 (3) ◽  
pp. 207-214
Author(s):  
Manju Rawat ◽  
Swarnlata Saraf
Keyword(s):  
Drug Delivery ◽  
Protein Stability ◽  
Biodegradable Polymers ◽  
Clinical Utility ◽  
Physicochemical Characteristics ◽  
Peptide Delivery ◽  
Chemical Instabilities ◽  
Physical And Chemical ◽  
Proteins And Peptides

Currently, drug delivery technologies for protein and peptide delivery mainly rely on biodegradable polymers. However, protein stability during release from these systems can be critical due to physical and chemical instabilities. Lipospheres are solid microparticles composed of fat core stabilized by phospholipids layer represent an alternative carrier for the delivery of highly challenging, labile and unstable  substances. This review highlights various aspects of lipospheres like physicochemical characteristics and stability for better clinical utility with a wider spectrum of proteins and peptides.

Synthesis of hepatitis B surface antigen pre-S2 region fragments and studies on their immunogenicity

1988 ◽  
Vol 53 (11) ◽  
pp. 2952-2956 ◽  
Author(s):  
Bernard Lammek ◽  
Zbigniew Maćkiewicz ◽  
Izabela Derdowska ◽  
Hanna Świderska ◽  
Adam Nowosławski ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Surface Antigen ◽  
Solid Phase ◽  
Hepatitis B Surface Antigen ◽  
Gel Filtration ◽  
Exchange Chromatography ◽  
Antigenic Determinants ◽  
Phase Method ◽  
Peptide Fragments ◽  
Humoral Immune

Two peptide fragments of hepatitis B surface antigen pre-S2 region were synthesized by the solid phase method. The peptides were purified by gel filtration or ion-exchange chromatography on Sephadex SP-C-25. Both peptides induced a cellular and humoral immune response in rabbits. The results showed that fragment 14-22 of pre-S2 region contains one of the antigenic determinants.

Magnetic Nanoparticles in Brain Disease Diagnosis and Targeting Drug Delivery

2011 ◽  
Vol 7 (1) ◽  
pp. 37-46 ◽  
Author(s):  
Xin Su ◽  
Xin Zhan ◽  
Fang Tang ◽  
Jingyuan Yao ◽  
Ji Wu
Keyword(s):  
Drug Delivery ◽  
Magnetic Nanoparticles ◽  
Disease Diagnosis ◽  
Brain Disease

scholarly journals Polymeric Drug Delivery System Based on Pluronics for Cancer Treatment

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3610
Author(s):  
Jialin Yu ◽  
Huayu Qiu ◽  
Shouchun Yin ◽  
Hebin Wang ◽  
Yang Li
Keyword(s):  
Drug Delivery ◽  
Self Assembly ◽  
Drug Delivery Systems ◽  
Tumor Therapy ◽  
Chemical Properties ◽  
Drug Carriers ◽  
Disease Diagnosis ◽  
Delivery Systems ◽  
Polymeric Drug Delivery ◽  
Physical And Chemical

Pluronic polymers (pluronics) are a unique class of synthetic triblock copolymers containing hydrophobic polypropylene oxide (PPO) and hydrophilic polyethylene oxide (PEO) arranged in the PEO-PPO-PEO manner. Due to their excellent biocompatibility and amphiphilic properties, pluronics are an ideal and promising biological material, which is widely used in drug delivery, disease diagnosis, and treatment, among other applications. Through self-assembly or in combination with other materials, pluronics can form nano carriers with different morphologies, representing a kind of multifunctional pharmaceutical excipients. In recent years, the utilization of pluronic-based multi-functional drug carriers in tumor treatment has become widespread, and various responsive drug carriers are designed according to the characteristics of the tumor microenvironment, resulting in major progress in tumor therapy. This review introduces the specific role of pluronic-based polymer drug delivery systems in tumor therapy, focusing on their physical and chemical properties as well as the design aspects of pluronic polymers. Finally, using newer literature reports, this review provides insights into the future potential and challenges posed by different pluronic-based polymer drug delivery systems in tumor therapy.

Solidification of Ionic Liquids: Theory and Techniques

2010 ◽  
Vol 63 (4) ◽  
pp. 544 ◽  
Author(s):  
Anja-Verena Mudring
Keyword(s):  
Phase Transition ◽  
Ionic Liquid ◽  
Ionic Liquids ◽  
Solid Phase ◽  
Soft Materials ◽  
Separation Science ◽  
Vast Number ◽  
Plastic Crystals ◽  
Thermal Fluids ◽  
Solid Phase Transition

Ionic liquids (ILs) have become an important class of solvents and soft materials over the past decades. Despite being salts built by discrete cations and anions, many of them are liquid at room temperature and below. They have been used in a wide variety of applications such as electrochemistry, separation science, chemical synthesis and catalysis, for breaking azeotropes, as thermal fluids, lubricants and additives, for gas storage, for cellulose processing, and photovoltaics. It has been realized that the true advantage of ILs is their modular character. Each specific cation–anion combination is characterized by a unique, characteristic set of chemical and physical properties. Although ILs have been known for roughly a century, they are still a novel class of compounds to exploit due to the vast number of possible ion combinations and one fundamental question remains still inadequately answered: why do certain salts like ILs have such a low melting point and do not crystallize readily? This Review aims to give an insight into the liquid–solid phase transition of ILs from the viewpoint of a solid-state chemist and hopes to contribute to a better understanding of this intriguing class of compounds. It will introduce the fundamental theories of liquid–solid-phase transition and crystallization from melt and solution. Aside form the formation of ideal crystals the development of solid phases with disorder and of lower order like plastic crystals and liquid crystals by ionic liquid compounds are addressed. The formation of ionic liquid glasses is discussed and finally practical techniques, strategies and methods for crystallization of ionic liquids are given.

Multivesicular liposome: A lipid-based drug delivery system for efficient drug delivery

2021 ◽  
Vol 27 ◽  
Author(s):  
Bapi Gorain ◽  
Bandar E. Al-Dhubiab ◽  
Anroop Nair ◽  
Prashant Kesharwani ◽  
Manisha Pandey ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Sustained Release ◽  
Delivery System ◽  
Targeted Delivery ◽  
Drug Loading ◽  
Therapeutic Agents ◽  
Laboratory Research ◽  
Efficacy And Safety ◽  
Liposomal Delivery ◽  
Proteins And Peptides

: The advancement of delivery tools for therapeutic agents has brought several novel formulations with increased drug loading, sustained release, targeted delivery, and prolonged efficacy. Amongst the several novel delivery approaches, multivesicular liposome has gained potential interest because this delivery system possesses the above advantages. In addition, this multivesicular liposomal delivery prevents degradation of the entrapped drug within the physiological environment while administered. The special structure of the vesicles allowed successful entrapment of hydrophobic and hydrophilic therapeutic agents, including proteins and peptides. Furthermore, this novel formulation could maintain the desired drug concentration in the plasma for a prolonged period, which helps to reduce the dosing frequencies, improve bioavailability, and safety. This tool could also provide stability of the formulation, and finally gaining patient compliance. Several multivesicular liposomes received approval for clinical research, while others are at different stages of laboratory research. In this review, we have focused on the preparation of multivesicular liposomes along with their application in different ailments for the improvement of the performance of the entrapped drug. Moreover, the challenges of delivering multivesicular vesicles have also been emphasized. Overall, it could be inferred that multivesicular liposomal delivery is a novel platform of advanced drug delivery with improved efficacy and safety.

scholarly journals Biophysical characterization of DNA origami nanostructures reveals inaccessibility to intercalation binding sites

2019 ◽  
Author(s):  
Helen L. Miller ◽  
Sonia Contera ◽  
Adam J.M. Wollman ◽  
Adam Hirst ◽  
Katherine E. Dunn ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Single Molecule ◽  
Targeted Drug Delivery ◽  
Binding Sites ◽  
Dna Origami ◽  
Target Cells ◽  
Dna Nanostructures ◽  
Synthetic Dna ◽  
Drug Molecules ◽  
Targeted Drug

AbstractIntercalation of drug molecules into synthetic DNA nanostructures formed through self-assembled origami has been postulated as a valuable future method for targeted drug delivery. This is due to the excellent biocompatibility of synthetic DNA nanostructures, and high potential for flexible programmability including facile drug release into or near to target cells. Such favourable properties may enable high initial loading and efficient release for a predictable number of drug molecules per nanostructure carrier, important for efficient delivery of safe and effective drug doses to minimise non-specific release away from target cells. However, basic questions remain as to how intercalation-mediated loading depends on the DNA carrier structure. Here we use the interaction of dyes YOYO-1 and acridine orange with a tightly-packed 2D DNA origami tile as a simple model system to investigate intercalation-mediated loading. We employed multiple biophysical techniques including single-molecule fluorescence microscopy, atomic force microscopy, gel electrophoresis and controllable damage using low temperature plasma on synthetic DNA origami samples. Our results indicate that not all potential DNA binding sites are accessible for dye intercalation, which has implications for future DNA nanostructures designed for targeted drug delivery.

scholarly journals Moleculary imprinted micro- and nanoparticles for cancer associated glycan motifs

2021 ◽  
Author(s):  
◽  
Liliia Mavliutova
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Cell Imaging ◽  
Functional Monomers ◽  
Crosslinked Polymer ◽  
Relative Contribution ◽  
Potential Applications ◽  
Specific Receptors ◽  
Cancer Glycosylation ◽  
Complementary Binding

Sialic acids are an important family of monosaccharides that are typically found as terminal moieties of glycans. Aberrant sialylation has been proven to correlate with various diseases including cancer. Glycosylation analysis is complex due to high diversityof the glycan isomers and their low abundance. Antibodies and lectins are commonly used in glycan purification and enrichment. However, high cost, poor availability, and limitation in storage/testing conditions hinders their application on a broader scale. This thesis is focused on the development of alternative glycan specific receptors with their potential applications in glycomics and cell imaging. The underlying technique for producing the synthetic receptors is molecular imprinting. Highly complementary binding sites are formed by fixing pre-ordered template/functional monomer complexes into a highly crosslinked polymer matrix. Fundamental investigation of this intermolecular imprinting approach in the imprinting of glycosylated targets is reported here. The core of this study focuses on the elucidation of relative contribution of orthogonally interacting functional monomers, their structural tuning and the importance of monomer, solvent and counterion choice on the imprinting. Molecularly imprinted polymers (MIPs) are developed as particles of different sizes for glycan/glycopeptide enrichment applications or combined with fluorescent reportergroups for use as glycan imaging nanolabels. Special attention is given to the improvement of sialic acid MIP selectivities toward particular structures associated with cancer biomarkers. Development of MIPs against such complex targets includes design of linkage selective MIPs with comprehensive studies of the affinities and selectivities of the final glycan specific materials.

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