scholarly journals Re-emergence of Gamma-like-II and emergence of Gamma-S:E661D SARS-CoV-2 lineages in the south of Brazil after the 2021 outbreak

2021 ◽  
Vol 18 (1) ◽  
Author(s):  
Mauro M. Oliveira ◽  
Michelle O. Schemberger ◽  
Andreia A. Suzukawa ◽  
Irina N. Riediger ◽  
Maria do Carmo Debur ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
The State ◽  
The Novel ◽  
High Frequencies ◽  
Variant Frequency ◽  
Northern Brazil ◽  
South Of Brazil ◽  
Amazonas State ◽  
Second Wave ◽  
Representative Samples

Abstract Background We report a genomic surveillance of SARS-CoV-2 lineages circulating in Paraná, southern Brazil, from March 2020 to April 2021. Our analysis, based on 333 genomes, revealed that the first variants detected in the state of Paraná in March 2020 were the B.1.1.33 and B.1.1.28 variants. The variants B.1.1.28 and B.1.1.33 were predominant throughout 2020 until the introduction of the variant P.2 in August 2020 and a variant of concern (VOC), Gamma (P.1), in January 2021. The VOC Gamma, a ramification of the B.1.1.28 lineage first detected in Manaus (northern Brazil), has grown rapidly since December 2020 and was thought to be responsible for the deadly second wave of COVID-19 throughout Brazil. Methods The 333 genomic sequences of SARS-CoV-2 from March 2020 to April 2021 were generated as part of the genomic surveillance carried out by Fiocruz in Brazil Genomahcov Fiocruz. SARS-CoV-2 sequencing was performed using representative samples from all geographic areas of Paraná. Phylogenetic analyses were performed using the 333 genomes also included other SARS-CoV-2 genomes from the state of Paraná and other states in Brazil that were deposited in the GISAID. In addition, the time-scaled phylogenetic tree was constructed with up to 3 random sequences of the Gamma variant from each state in Brazil in each month of 2021. In this analysis we also added the sequences identified as the B.1.1.28 lineage of the Amazonas state and and the Gamma-like-II (P.1-like-II) lineage identified in different regions of Brazil. Results Phylogenetic analyses of the SARS-CoV-2 genomes that were previously classified as the VOC Gamma lineage by WHO/PANGO showed that some genomes from February to April 2021 branched in a monophyletic clade and that these samples grouped together with genomes recently described with the lineage Gamma-like-II. Additionally, a new mutation (E661D) in the spike (S) protein has been identified in nearly 10% of the genomes classified as the VOC Gamma from Paraná in March and April 2021.Finally, we analyzed the correlation between the lineage and the Gamma variant frequency, age group (patients younger or older than 60 years old) and the clinical data of 86 cases from the state of Paraná. Conclusions Our results provided a reliable picture of the evolution of the SARS-CoV-2 pandemic in the state of Paraná characterized by the dominance of the Gamma strain, as well as a high frequencies of the Gamma-like-II lineage and the S:E661D mutation. Epidemiological and genomic surveillance efforts should be continued to unveil the biological relevance of the novel mutations detected in the VOC Gamma in Paraná.

scholarly journals Genomic surveillance of SARS-CoV-2 in the state of Paraná, Southern Brazil, reveals the cocirculation of the VOC P.1, P.1-like-II lineage and a P.1 cluster harboring the S:E661D mutation

2021 ◽  
Author(s):  
Mauro de Medeiros Oliveira ◽  
Michelle Orane Schemberger ◽  
Andreia Akemi Suzukawa ◽  
Irina Nastassja Riediger ◽  
Maria do Carmo Debur ◽  
...  
Keyword(s):  
High Frequency ◽  
Phylogenetic Analyses ◽  
The State ◽  
The Novel ◽  
Southern Brazil ◽  
Monophyletic Clade ◽  
Novel Mutations ◽  
New Mutation ◽  
High Frequencies ◽  
Variant Frequency

We report a genomic surveillance of SARS-CoV-2 lineages circulating in Paraná, Southern Brazil, from March 2020 to April 2021. Our analysis, based on 333 genomes, revealed that the first variants detected in the state of Paraná in March 2020 were the B.1.1.33 and B.1.1.28 variants. The variants B.1.1.28 and B.1.1.33 were predominant throughout 2020 until the introduction of the variant P.2 in August 2020 and a variant of concern (VOC), P.1, in January 2021. Phylogenetic analyses of the SARS-CoV-2 genomes that were previously classified as the VOC P.1 lineage by PANGO showed that some genomes from February to April 2021 branched in a monophyletic clade and that these samples grouped together with genomes recently described with the lineage P.1-like-II. An extended phylogenetic analysis, including SARS-CoV-2 genomes from all over Brazil, showed that the P.1-like-II lineage appears at a high frequency in the southern region of the country. The P.1-like-II lineage genomes share some, but not all, defining mutations of the VOC P.1. For instance, it has the previously described ORF1a:D2980H and N:P383 L unique mutations and the newly detected ORF1a:P1213 L and ORF1b:K2340N mutations. Additionally, a new mutation (E661D) in the spike (S) protein has been identified in nearly 10\% of the genomes classified as the VOC P.1 from Paraná in March and April 2021. We also report the identification of the S:W152C mutation in one genome from Paraná, classified as the N.10 variant. Finally, we analyzed the correlation between the lineage and the P.1 variant frequency, age group (patients younger or older than 60 years old) and the clinical data of 86 cases from the state of Paraná. This analysis does not support an association between the P.1 variant prevalence and COVID-19 severity or age strata. Our results provided a reliable picture of the evolution of the SARS-CoV-2 pandemic in the state of Paraná characterized by the dominance of the P.1 strain, as well as a high frequencies of the P.1-like-II lineage and the S:E661D mutations. Epidemiological and genomic surveillance efforts should be continued to unveil the biological relevance of the novel mutations detected in the VOC P.1 in Paraná.

scholarly journals Evolution of mortality and lethality due to covid-19 in the state of Roraima, Brazil, from march 2020 to july 2021

2021 ◽  
Vol 31 (3) ◽  
pp. 447-457
Author(s):  
Edwin Vivanco Valenzuela ◽  
Tassiane Cristina de Morais ◽  
Blanca Guerrero Daboin ◽  
Matheus Paiva Emídio Cavalcanti ◽  
Isabella Batista Martins Portugal ◽  
...  
Keyword(s):  
Time Series ◽  
Incidence Rate ◽  
Reduction Rate ◽  
Secondary Data ◽  
The State ◽  
P Value ◽  
Northern Brazil ◽  
Poverty Indicators ◽  
Increasing Trends ◽  
Second Wave

Introduction: the context of the Covid-19 pandemic in the Brazilian North region is worrying. There is a lack of resources for Public Health, a low human development index, and poverty indicators above the national average. Objective: to analyze mortality and lethality from COVID-19 in the State of Roraima, Brazil. Methods: this is an ecological time-series study of secondary data on COVID-19 in Roraima, Northern Brazil, from March 2020 to July 2021. The incidence, mortality, and lethality rates due to COVID-19 were calculated. The Prais-Winsten regression model was used to calculate the time series trends. Trends were classified as increasing, decreasing, or stationary. The trend was considered static when the p-value was not significant (p>0.05). Results: in the state of Roraima, from March 2020 to July 2021, there were 123,125 cases and 1,903 accumulated deaths due to COVID-19. The first wave (March 2020 to October 2021) of COVID-19 recorded the incidence rate (2,995.30 new cases per 100,000 inhabitants - July 2020) and mortality (56.32 deaths per 100,000 inhabitants - June 2020) higher lethality. However, in the second wave (November 2020 to July 2021), the highest lethality rate was observed (3.47% - February 2021). It was observed that during the first wave, the incidence rate of COVID-19 showed increasing trends. During this period, the mortality rate had a stationary tendency (p>0.05) and the percentage lethality with a decreasing trend (p<0.05). During the second wave, there was a more aggravating scenario for lethality, which changed from a daily reduction rate of 0.90% to stationary trends. Conclusion: the pandemic in the state of Roraima is not yet under control, so it is necessary to strengthen strategies to mitigate the spread of the pandemic in the region and prevent the formation of new waves.

scholarly journals First record of the caddisfly Notidobiella amazoniana Holzenthal & Blahnik, 2010 (Trichoptera, Sericostomatidae) from Roraima state, northern Brazil

Check List ◽  
2020 ◽  
Vol 16 (3) ◽  
pp. 661-664
Author(s):  
Gleison R. Desidério ◽  
Ana M. Pes
Keyword(s):  
South America ◽  
First Record ◽  
Geographic Range ◽  
The State ◽  
Type Locality ◽  
Distribution Map ◽  
The Family ◽  
Northern Brazil ◽  
Amazonas State

Notidobiella amazoniana Holzenthal &amp; Blahnik, 2010 was known only from the type locality in Amazonas state, Brazil. Herein, we provide the first record of N. amazoniana for Roraima state, thus extending the geographic range of this species to the northernmost state of Brazil. Its occurrence in Roraima also represents the first record of the family Sericostomatidae for the state and the most northern record of the genus in South America. Information on the holotype of N. amazoniana and a distribution map for all species of Notidobiella are provided.

Untimely Development, Ugly History:A Drama in Muslinand the Rejection of National-Historical Time

2013 ◽  
Vol 3 (2) ◽  
pp. 161-183
Author(s):  
Mary L. Mullen
Keyword(s):  
Everyday Life ◽  
The State ◽  
The Political ◽  
Nation State ◽  
The Novel ◽  
Historical Time ◽  
Political Processes ◽  
The Core

This article considers the politics and aesthetics of the colonial Bildungsroman by reading George Moore's often-overlooked novel A Drama in Muslin (1886). It argues that the colonial Bildungsroman does not simply register difference from the metropolitan novel of development or express tension between the core and periphery, as Jed Esty suggests, but rather can imagine a heterogeneous historical time that does not find its end in the nation-state. A Drama in Muslin combines naturalist and realist modes, and moves between Ireland and England to construct a form of untimely development that emphasises political processes (dissent, negotiation) rather than political forms (the state, the nation). Ultimately, the messy, discordant history represented in the novel shows the political potential of anachronism as it celebrates the untimeliness of everyday life.

Cyclone Bomb hits Southern Brazil in 2020

2020 ◽  
Vol 3 (3) ◽  
Author(s):  
Ricardo Gobato ◽  
Alireza Heidari
Keyword(s):  
High Pressure ◽  
Atmospheric Pressure ◽  
High Speed ◽  
Satellite Images ◽  
The State ◽  
Southern Brazil ◽  
Santa Catarina ◽  
High Area ◽  
South Of Brazil ◽  
Rapid Drop

An “explosive extratropical cyclone” is an atmospheric phenomenon that occurs when there is a very rapid drop in central atmospheric pressure. This phenomenon, with its characteristic of rapidly lowering the pressure in its interior, generates very intense winds and for this reason it is called explosive cyclone, bomb cyclone. With gusts recorded of 116 km/h, atmospheric phenomenon – “cyclone bomb” (CB) hit southern Brazil on June 30, the beginning of winter 2020, causing destruction in its influence over. One of the cities most affected was Chapecó, west of the state of Santa Catarina. The satellite images show that the CB generated a low pressure (976 mbar) inside it, generating two atmospheric currents that moved at high speed. In a northwest-southeast direction, Bolivia and Paraguay, crossing the states of Parana and Santa Catarina, and this draft that hit the south of Brazil, which caused the destruction of the affected states.  Another moving to Argentina, southwest-northeast direction, due to high area of high pressure (1022 mbar). Both enhanced the phenomenon.

Who’s holding up the sky? On Rymba, a novel by A. Bushkovsky

2020 ◽  
Vol 1 (1) ◽  
pp. 150-158
Author(s):  
A. V. Zhuchkova
Keyword(s):  
Gender Politics ◽  
The State ◽  
Christian Faith ◽  
The Novel ◽  
Central Question ◽  
Men And Women ◽  
Flood Zone ◽  
The Social ◽  
Russian North ◽  
And Gender

The article deals with A. Bushkovsky’s novel Rymba that goes beyond the topics typical of Russian North prose. Rather than limiting himself to admiring nature and Russian character, the author portrays the northern Russian village of Rymba in the larger context of the country’s mentality, history, mythology, and gender politics. In the novel, myth clashes with reality, history with the present day, and an individual with the state. The critic draws a comparison between the novel and the traditions of village prose and Russian North prose. In particular, Bushkovsky’s Rymba is discussed alongside V. Rasputin’s Farewell to Matyora [ Proshchanie s Matyoroy ] and R. Senchin’s The Flood Zone [ Zona zatopleniya ]. The novel’s central question is: what keeps the Russian world afloat? Depicting the Christian faith as such a bulwark, Bushkovsky links atheism with the social and spiritual roles played by contemporary men and women. The critic argues, however, that the reliance on Christianity in the novel verges on an affectation. The book’s main symbol is a drowning hawk: it perishes despite people’s efforts to save it.

The reality of translating literature from Hebrew to Arabic The novel as a model

2020 ◽  
pp. 149-164
Author(s):  
أميرة عبد الحفيظ عمارة
Keyword(s):  
Financial Support ◽  
Academic Research ◽  
Arab Countries ◽  
The State ◽  
The Novel ◽  
Peace Agreement ◽  
Existential Anxiety ◽  
Publishing Houses ◽  
Arab Descent ◽  
State Of Israel

This research is interested in studying the reality of translation from Hebrew to Arabic, especially the translation of novels. The research relied on translated and published novels, from certain publishing houses, and it includes about 29 novels translated from Hebrew to Arabic. The first translation in this field was Ahavat Zion )loving Zion(, a novel by Abraham Mapu (1808-1867), translated by Salim Al-Dawoodi, and published by the Al-khidewiah Press in Cairo in 1899. Translations from Hebrew to, and vice versa, had Flourished after the establishment of the State of Israel, in particular after 1967 War, and resumed after the peace agreement with Israel. The largest wave of such translations was carried out in newspapers, magazines and academic research in part. The eighties and nineties of the last century were a period of translation activity in regard of partial translations in newspapers. The numbers of translations of full novels published so far have not exceeded thirty in most cases, and the number of translations published in Israel is approximate to the translations published in the Arab countries. The trends of novels that were translated inside Israel were of specific trends, and the translated works that were chosen were initiated, encouraged, and financed by organizations supported by the Israeli establishment. In addition, the translators also had a role in choosing the translated novels into Arabic to obtain financial support. As for the translated Hebrew works in the Arab countries, their focus was on the conditions and sufferings of the Israelis from Arab descent in Israel, and on the failure of Zionism and the issues of existential anxiety the Israelis are experiencing.

scholarly journals Sequence of a Coxiella endosymbiont of the tick Amblyomma nuttalli suggests a pattern of convergent genome reduction in the Coxiella genus

2020 ◽  
Author(s):  
Tiago Nardi ◽  
Emanuela Olivieri ◽  
Edward Kariuki ◽  
Davide Sassera ◽  
Michele Castelli
Keyword(s):  
Coxiella Burnetii ◽  
Phylogenetic Analyses ◽  
Genome Reduction ◽  
Metabolic Profiles ◽  
The Novel ◽  
Bacterial Symbionts ◽  
Human Pathogen ◽  
Host Genus ◽  
Supportive Role ◽  
Genus One

Abstract Ticks require bacterial symbionts for the provision of necessary compounds that are absent in their hematophagous diet. Such symbionts are frequently vertically transmitted and, most commonly, belong to the Coxiella genus, which also includes the human pathogen Coxiella burnetii. This genus can be divided in four main clades, presenting partial but incomplete co-cladogenesis with the tick hosts. Here we report the genome sequence of a novel Coxiella, endosymbiont of the African tick Amblyomma nuttalli, and the ensuing comparative analyses. Its size (~1 Mb) is intermediate between symbionts of Rhipicephalus species and other Amblyomma species. Phylogenetic analyses show that the novel sequence is the first genome of the B clade, the only one for which no genomes were previously available. Accordingly, it allows to draw an enhanced scenario of the evolution of the genus, one of parallel genome reduction of different endosymbiont lineages, which are now at different stages of reduction from a more versatile ancestor. Gene content comparison allows to infer that the ancestor could be reminiscent of Coxiella burnetii. Interestingly, the convergent loss of mismatch repair could have been a major driver of such reductive evolution. Predicted metabolic profiles are rather homogenous among Coxiella endosymbionts, in particular vitamin biosynthesis, consistently with a host-supportive role. Concurrently, similarities among Coxiella endosymbionts according to host genus and despite phylogenetic unrelatedness hint at possible host-dependent effects.

scholarly journals The Second Wave of COVID-19: Clinical Pharmacy Services During a Field Hospital Operation

2021 ◽  
pp. 001857872110323
Author(s):  
Jessica Mazzone ◽  
Krysta Shannon ◽  
Richard Rovelli ◽  
Racha Kabbani ◽  
Angel Amaral ◽  
...  
Keyword(s):  
Clinical Pharmacy ◽  
The State ◽  
Pharmacy Services ◽  
Newly Diagnosed ◽  
Field Hospital ◽  
Clinical Role ◽  
Diagnosed Diabetes ◽  
Newly Diagnosed Diabetes ◽  
Second Wave

The second wave of COVID-19 emerged in the late fall months in the state of Massachusetts and inadvertently caused a rise in the number of cases requiring hospitalization. With a field hospital previously opened in central Massachusetts during the Spring of 2020, the governor decided to reimplement the field hospital. Although operations were effectively accomplished during the first wave, the reimplementation of the field hospital came with its new set of challenges for operating a satellite pharmacy. Experiences gathered include new pharmacy operation workflows, the clinical role of pharmacy services, introduction of remdesivir treatment, and pharmacy involvement in newly diagnosed diabetes patients requiring insulin teaching. Pharmacy services were successful in adapting to the rapidly growing number in patients with a total of over 600 patients served in a course of 2 months.

scholarly journals Cryptosporidium myocastoris n. sp. (Apicomplexa: Cryptosporidiidae), the Species Adapted to the Nutria (Myocastor coypus)

2021 ◽  
Vol 9 (4) ◽  
pp. 813
Author(s):  
Jana Ježková ◽  
Zlata Limpouchová ◽  
Jitka Prediger ◽  
Nikola Holubová ◽  
Bohumil Sak ◽  
...  
Keyword(s):  
Phylogenetic Analyses ◽  
Clinical Signs ◽  
Prepatent Period ◽  
Cryptosporidium Species ◽  
The Novel ◽  
Myocastor Coypus ◽  
The Czech Republic ◽  
Molecular Analyses ◽  
Cryptosporidium Spp ◽  
Faecal Samples

Cryptosporidium spp., common parasites of vertebrates, remain poorly studied in wildlife. This study describes the novel Cryptosporidium species adapted to nutrias (Myocastor coypus). A total of 150 faecal samples of feral nutria were collected from locations in the Czech Republic and Slovakia and examined for Cryptosporidium spp. oocysts and specific DNA at the SSU, actin, HSP70, and gp60 loci. Molecular analyses revealed the presence of C. parvum (n = 1), C. ubiquitum subtype family XIId (n = 5) and Cryptosporidium myocastoris n. sp. XXIIa (n = 2), and XXIIb (n = 3). Only nutrias positive for C. myocastoris shed microscopically detectable oocysts, which measured 4.8–5.2 × 4.7–5.0 µm, and oocysts were infectious for experimentally infected nutrias with a prepatent period of 5–6 days, although not for mice, gerbils, or chickens. The infection was localised in jejunum and ileum without observable macroscopic changes. The microvilli adjacent to attached stages responded by elongating. Clinical signs were not observed in naturally or experimentally infected nutrias. Phylogenetic analyses at SSU, actin, and HSP70 loci demonstrated that C. myocastoris n. sp. is distinct from other valid Cryptosporidium species.

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