scholarly journals In-Vitro antioxidant, anti-lipid peroxidative activities and In-Silico study of Terminalia chebula bioactive compounds

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Syed Mubashar Sabir ◽  
Syed Rizwan Abbas ◽  
Shabnam Shahida ◽  
Muhammad Faraz Khan
Keyword(s):  
Lipid Peroxidation ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Protein Docking ◽  
Hot Water ◽  
Antioxidant Compounds ◽  
Terminalia Chebula ◽  
String Analysis ◽  
Anti Inflammation

Abstract Objective To evaluate the antioxidant activities and to identify the bioactive compounds in hot water extracts of Terminalia chebula fruit. Methods The antioxidant activities were determined by DPPH assay, lipid peroxidation assay, iron chelation and total antioxidant assay. The phenolic composition was determined by HPLC-DAD. Human Rab8b Protein was used for the validation of compounds as anti-inflammation. String analysis for protein synergism was used. Results The analysis of Terminalia chebula Retzius (Combretaceae) phenolics showed anti-inflammatory effect. The specific phenolic compositions were determined by high performance liquid chromatography (HPLC) and resulted in the identification of rutin, catechin, caffeic acid, gallicacid, ellagic acid, epicatechin, and quercetin as antioxidant compounds. Human Rab8b protein is selected for protein docking and all compounds except rutin showed good results. ADMET properties were checked by using AdmetSar and all seven compounds showed validation for AMET properties. The synergisms of compounds were analyzed by STRING analysis and our ligands shows strong binding with human Rab8b proteins. The aqueous extract was capable of inhibiting the lipid peroxidation in egg yolk phospholipid homogenate. The extract scavenged the 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) (IC50,71.5 ± 2.1 μg/ml). The extract displayed the high metal chelation activities and reducing abilities on the phosphomolybdenum assay. Conclusions It is concluded that extracts of T. chebula have good antioxidant and anti-inflammation activities and are rich in phenolics.

Antioxidant activities and lipid peroxidation status in human follicular fluid: age-dependent change

Zygote ◽  
2021 ◽  
pp. 1-5
Author(s):  
H. Debbarh ◽  
N. Louanjli ◽  
S. Aboulmaouahib ◽  
M. Jamil ◽  
L. Ahbbas ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Follicular Fluid ◽  
Maternal Age ◽  
Antioxidant Activities ◽  
Human Follicular Fluid ◽  
Age Related ◽  
Group I ◽  
Group Ii ◽  
Scavenging Efficiency

Summary Maternal age is a significant factor influencing in vitro fertilization (IVF) outcomes. Oxidative stress (OS) is one of the major causes of age-related cellular and molecular damage. The purpose of this work was to investigate the correlation between maternal age with intrafollicular antioxidants and OS markers in follicular fluid (FF), and also to determine the OS status in patients of advanced age. This study was a prospective study including 201 women undergoing IVF whose age was between 24 and 45 years old. FF samples were obtained from mature follicles at the time of oocyte retrieval. After treatment of FF, lipid peroxidation levels (MDA) and enzyme activities such as superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GR) and glutathione (GSH) level were evaluated using spectrophotometry. The results indicated that the age cutoff point for increasing the MDA level was fixed at 37 years, allowing the study to be differentiated into two age groups. Group I included patients whose age was less than 37 years, and group II included patients whose age was greater than or equal 37 years. Statistical analysis revealed that MDA and GSH levels and GR activity were significantly higher in group II compared with group I. The SOD and CAT activities were significantly less in group II compared with group I. We concluded that from 37 years old a reproductive ageing was accompanied by a change in the antioxidant pattern in FF that impaired reactive oxygen species scavenging efficiency.

Radioprotective effects of active compounds of Acanthopanax senticosus from the Lesser Khingan Mountain range in China

RSC Advances ◽  
2016 ◽  
Vol 6 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Wei Song ◽  
Jinming Shi ◽  
Denis Baranenko ◽  
Jing Jing ◽  
Weihong Lu
Keyword(s):  
Bioactive Compounds ◽  
Radiation Injury ◽  
Antioxidant Activities ◽  
Mountain Range ◽  
Acanthopanax Senticosus ◽  
Active Compounds

Bioactive compounds extracted from wild Acanthopanax senticosus showed radioprotective effects through in vitro antioxidant activities and suppression of radiation injury in mice.

Phytochemical analysis and nephroprotective effect of cactus (Opuntia ficus-indica) cladodes on sodium dichromate-induced kidney injury in rats

2019 ◽  
Vol 44 (3) ◽  
pp. 239-247
Author(s):  
Mbarka Hfaiedh ◽  
Dalel Brahmi ◽  
Mohamed Nizar Zourgui ◽  
Lazhar Zourgui
Keyword(s):  
Lipid Peroxidation ◽  
Ascorbic Acid ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Wistar Rat ◽  
Phytochemical Analysis ◽  
Sodium Dichromate ◽  
Opuntia Ficus Indica

Environmental and occupational exposure to chromium compounds, especially hexavalent chromium, is widely recognized as potentially nephrotoxic in humans and animals. The present study aimed to assess the efficacy of cactus (Opuntia ficus-indica) against sodium dichromate-induced nephrotoxicity, oxidative stress, and genotoxicity. Cactus cladodes extract (CCE) was phytochemically studied and tested in vitro for its potential antioxidant activities. Additionally, the preventive effect of CCE against sodium dichromate-induced renal dysfunction in a Wistar rat model (24 rats) was evaluated. For this purpose, CCE at a dose of 100 mg/kg was orally administered, followed by 10 mg/kg sodium dichromate (intraperitoneal injection). After 40 days of treatment, the rats were sacrificed, and the kidneys were excised for histological, lipid peroxidation, and antioxidant enzyme analyses. The phenol, flavonoid, tannin, ascorbic acid, and carotenoid contents of CCE were considered to be important. Our analyses showed that 1 mL of CCE was equivalent to 982.5 ± 1.79 μg of gallic acid, 294.37 ± 0.84 μg of rutin, 234.78 ± 0.24 μg of catechin, 204.34 ± 1.53 μg of ascorbic acid, and 3.14 ± 0.51 μg of β-carotene. In vivo, pretreatment with CCE was found to provide significant protection against sodium dichromate-induced nephrotoxicity by inhibiting lipid peroxidation, preserving normal antioxidant activities, and protecting renal tissues from lesions and DNA damage. The nephroprotective potential of CCE against sodium dichromate toxicity might be due to its antioxidant properties.

scholarly journals Analysis of the oxidative stress inhibition potentials of Artemisia annua and its bioactive compounds through in vitro and in silico studies

2021 ◽  
Vol 18 (3) ◽  
pp. 245-259
Author(s):  
Stephen Adakole Ejembi ◽  
Titilayo Omolara Johnson ◽  
Jonathan Dingkwoet Dabak ◽  
Augustina Oduje Akinsanmi ◽  
Jane-Rose Ifuanyachi Oche ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bioactive Compounds ◽  
Artemisia Annua ◽  
Antioxidant Activities ◽  
Protein Targets ◽  
Active Involvement ◽  
In Silico Studies ◽  
Plateau State ◽  
Antioxidant Mechanisms

Oxidative stress overwhelms the antioxidant mechanisms of living systems, with active involvement in the pathogenesis of several diseases. Natives of Gangnim in the Plateau State of Nigeria may be unknowingly endowed with some protective advantages against oxidative stress for their habitual consumption of Artemisia annua tea. The antioxidant activities of A. annua extracts were determined using in vitro methods and the inhibitory potentials of twenty-nine (29) bioactive compounds of the plant against oxidative stress target proteins were assessed through molecular docking analysis. These extracts showed significantly high activities in scavenging nitric oxide, 2,2-diphenyl-1-picrylhydrazyl (DPPH) and reducing ferric (Fe3+) to ferrous (Fe2+) iron. Virtually, none of the bioactive compounds binds to the active site of the antioxidant protein targets. Rather, 72.41, 93.10 and 75.86% of these compounds bind with high binding affinity to the activator binding sites of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT) respectively. 7,8-dimethylalloxazine (-8.10 kcal/mol) ranked highest as a prospective inhibitor of xanthine oxidase (XOX). The antioxidant activity exhibited by the extracts of the locally cultivated A. annua and the molecular interactions of its bioactive compounds against the protein targets used predict that oxidative stress inhibition could be effectively achieved with these phytochemicals.

scholarly journals Enhanced Recovery of Antioxidant Compounds from Hazelnut (Corylus avellana L.) Involucre Based on Extraction Optimization: Phytochemical Profile and Biological Activities

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 460 ◽  
Author(s):  
Rusu ◽  
Fizeșan ◽  
Pop ◽  
Gheldiu ◽  
Mocan ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Enhanced Recovery ◽  
Biological Activities ◽  
Natural Antioxidants ◽  
Antioxidant Compounds ◽  
Cancerous Cell ◽  
Wide Range ◽  
Quantitative Analyses ◽  
Corylus Avellana L

Tree nut by-products could contain a wide range of phytochemicals, natural antioxidants, which might be used as a natural source for dietary supplements. The aim of the present study was to evaluate the phenolic and sterolic composition, as well as the antioxidant and other biological activities, of hazelnut involucre (HI) extracts. Experimental designs were developed in order to select the optimum extraction conditions (solvent, temperature, time) using turbo-extraction by Ultra-Turrax for obtaining extracts rich in bioactive compounds. Qualitative and quantitative analyses were performed by LC-MS and LC-MS/MS and they revealed important amounts of individual polyphenols and phytosterols, molecules with antioxidant potential. The richest polyphenolic HI extract with the highest antioxidant activity by TEAC assay was further evaluated by other in vitro antioxidant tests (DPPH, FRAP) and enzyme inhibitory assays. Additionally, the cytotoxic and antioxidant effects of this extract on two cancerous cell lines and on normal cells were tested. This is the first study to analyze the composition of both hydrophilic and lipophilic bioactive compounds in HI extracts. Our findings reveal that this plant by-product presents strong biological activities, justifying further research, and it could be considered an inexpensive source of natural antioxidants for food, pharmaceutical, or cosmetic industry.

scholarly journals Black goji berry (Lycium ruthenicum) tea has higher phytochemical contents and in vitro antioxidant properties than red goji berry (Lycium barbarum) tea

2020 ◽  
Vol 4 (4) ◽  
pp. 193-201
Author(s):  
Bei Liu ◽  
Qingqing Xu ◽  
Yujing Sun
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Antioxidant Properties ◽  
Antioxidant Property ◽  
Lycium Barbarum ◽  
Antioxidant Power ◽  
Goji Berry ◽  
Good Habit

Abstract Goji berry tea, a traditional herbal tea, is the main ate mode of goji berry in Asia, yet few studies in comparison with red goji berry tea and black goji berry tea are carried out. This study investigated the effects of water temperature and soak time on the colour, phytochemicals, and the antioxidant capacity [2,2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), and the ferric-reducing antioxidant power (FRAP)] of two goji berry tea. A comparison of the bioactive compounds and antioxidant activities between black and red goji berry tea was conducted. Results showed that both red and black goji berry tea were rich in phytochemicals, giving high antioxidant ability. The levels of bioactive compounds and the antioxidant activity of the two goji berry tea increased as the increases in soak temperature and time. Black goji berry tea had higher phytochemicals and antioxidant property than those of red goji berry tea. Infused at 100° water for the same time, the levels of total polysaccharides (150 mg/100 ml), total polyphenols (238 mg/ml), and antioxidant capacity (550 μmol/100 ml) of black goji berry tea were 3.5, 2, and 5 times higher, respectively, in comparison with red goji berry tea. The results of this study demonstrate that hot drink of goji berry in China is a good habit and black goji berry tea may be a better choice.

scholarly journals Chemical Composition, Antioxidant and Enzyme Inhibitory Properties of Different Extracts Obtained from Spent Coffee Ground and Coffee Silverskin

Foods ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 713 ◽  
Author(s):  
Gokhan Zengin ◽  
Kouadio Ibrahime Sinan ◽  
Mohamad Fawzi Mahomoodally ◽  
Simone Angeloni ◽  
Ahmed M. Mustafa ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Coffee Silverskin ◽  
Methanolic Extracts ◽  
Waste Production ◽  
Starting Point ◽  
Spent Coffee Ground ◽  
Coffee Ground ◽  
Inhibitory Activities

In a world where an eco-friendlier approach is becoming more and more necessary, it is essential to reduce waste production and to reuse residues of the company’s supply chain. Coffee silverskin (CS) and spent coffee ground (SCG), two by-products of coffee production, are important sources of bioactive compounds and, for this, some authors have proposed their reuse in the nutraceutical, food, and cosmetic sector. However, their potential enzyme inhibitory properties have been poorly investigated. Hence, the objective of the current work was to study the enzymatic inhibitory activities against acetylcholinesterase, butyrylcholinesterase, α-amylase, α-glucosidase, and tyrosinase of different extracts of CS and SCG. Before these in vitro bioassays, the phytochemical composition of each extract was investigated via colorimetric assays and HPLC-MS/MS analysis. In addition, the antioxidant activities were evaluated by different chemical approaches. SCG extracts contained a higher content of bioactive compounds, notably the SCG EtOH:H2O extract was the richest in caffeine and possessed the highest antioxidant activities. The hydroalcoholic and methanolic extracts were shown to be the most active against all tested enzymes, while the water extracts displayed lower activity. Our results showed a weak correlation between bioactive compounds and enzyme inhibitory effects, proving inhibitory activities likely due to non-phenolic molecules such as alkaloids and terpenoids. Obtained findings could be a starting point to develop novel nutraceuticals from CS and SCG.

scholarly journals Fungal-Mediated Biotransformation of Soybean Supplemented with Different Cereal Grains into a Functional Compound with Antioxidant, Anti-Inflammatory and Antitumoral Activities

2020 ◽  
Vol 11 (1) ◽  
pp. 8018-8033
Keyword(s):  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Brown Rice ◽  
Value Added ◽  
Cereal Grains ◽  
Antioxidant Compounds ◽  
A Value ◽  
Biotransformation Process ◽  
Soybean Fermentation ◽  
Anti Inflammatory

The aim of this study was to select fungal strains and alternative substrates to increase the production of bioactive compounds by solid-state bioprocessing using soybeans. Initially, from a total of 17 fungi strains, R. oligosporus NRRL 3267, R. oligosporus NRRL 2710, and R. arrhizus NRRL 2582 were pre-selected for presenting the greatest antioxidant activities during soybean fermentation. The three Rhizopus strains were cultured in soybeans supplemented with different cereal grains (brown rice, wheat, corn, and oat) aiming to achieve a higher antioxidant activity. Soybean supplementation with brown rice increased the concentration of phenolic compounds (0.697 to 6.447 mg GAE g-1) significantly compared with the only use of soybean (1.792 to 3.10 mg GAE g-1), using R. oligosporus NRRL 2710. The improved fungal-mediated biotransformation process (R. oligosporus NRRL 2710 with soybeans and brown rice) generated an isoflavone aglycone-rich product, containing different antioxidant compounds, such as trans-cinnamic acid, gallic acid, myricetin, quercetin, and kaempferol. The fermented substrate also showed great potential to inhibit hyaluronidase enzyme (anti-inflammatory activity) and against CaCo-2 tumor cells growth (antitumor activity). The resulting compound could serve as a value-added food and/or feed ingredient and a rich source of bioactive compounds.

scholarly journals Improvement on high-cholesterol diet induced atherosclerosis, lipid profile, oxidative stress and genotoxicity in the liver of mice by Echinops spinosissimus Turra subsp. spinosus

2020 ◽  
Author(s):  
Donyez Frikha Dammak ◽  
Hajer Ben Saad ◽  
Emna Bouattour ◽  
Ons Boudawara ◽  
Raoudha Mezghani Jarraya
Keyword(s):  
Oxidative Stress ◽  
Antioxidant Activities ◽  
Dietary Cholesterol ◽  
Atherogenic Index ◽  
High Cholesterol Diet ◽  
Antioxidant Compounds ◽  
Cholesterol Diet ◽  
High Cholesterol ◽  
Oxidative Stress Biomarkers

Abstract Background Hypercholesterolemia is a major risk factor for the development of atherosclerosis and endothelial dysfunction. Methods The present study investigates the possible mechanism of Echinops spinosissimus Turra subsp. spinosus ( E. s. spinosus ) flower on the high cholesterol diet. Results Our in vitro results demonstrated the richness of E.s. spinosus flower in antioxidant compounds, and its antioxidant activities. The co-administration of E.s. spinosus (100 or 200 mg/kg/day) with high-fat diet attenuated hepatotoxicity as monitored by the improvement of oxidative stress biomarkers and plasma lipid and liver parameters, when compared to the hypercholesterolemic mice. Atherogenic index and body weight were also reduced markedly, compared to control mice. These results were confirmed by the improvement of histological changes and DNA damage. Conclusion These data indicate that E.s. spinosus flower reduces the hypercholesterolemia risk and atherogenic properties of dietary cholesterol. Its hypocholesterolemic effect may be due to its antioxidant activities and its richness in bioactive molecule.

Sign in / Sign up

Export Citation Format

Share Document