scholarly journals The Effects of Starvation and Surgical Injury on the Plasma Levels of Glucose, Free Fatty Acids, and Neutral Lipids in Newborn Babies Suffering from Various Congenital Anomalies

1981 ◽  
Vol 15 (4) ◽  
pp. 313-318 ◽  
Author(s):  
M C Elphick ◽  
A W Wilkinson
1969 ◽  
Vol 52 (5) ◽  
pp. 904-910 ◽  
Author(s):  
Garnett Wood ◽  
Lane Hintz ◽  
Harold Salwin

Abstract Chemical changes that occur in the proteins, nucleotides, and lipids of fish tissue during storage at low temperatures were investigated. Homogenized tissue, prepared from fresh rock-fish (striped hass, Roccus species), was stored up to six days at temperatures from -10° to 4°C and then analyzed. At 0°C and below, the solubility of myofibrillar proteins decreased. There were also changes in polyacrylamide gel electrophoretic patterns of protein extracts. The total nucleotide content decreased rapidly at all temperatures. The lipids were extracted from each sample and separated into neutral lipids, phospholipids, and free fatty acids by column chromatography. The fatty acid composition of each fraction was determined by gas chromatography. In the fresh tissue, polyunsaturated acids occurred in greatest proportion in the free fatty acid and phospholipid fractions, whereas inono-unsaturated acids were inofe highly concentrated in the neutral lipids. The percentages of saturated acids were approximately the same in all fractions. During storage, there were considerably larger losses of individual acids from phospholipids than from neutral lipids. The polyunsaturated acids of the phospholipid fraction were affected most. Over 10% of these aeids were lost in six days at ice temperature, but only a small proportion of the losses was accounted for by increases in free fatty acids. Oxidative proo esses may account for the imbalance because the rate of oxidation, as measured by the thio-barbituric acid test, increased with storage temperature in the same manner as the rale at which unsaturated fatty acids were lost from the pliospliolipuls. Losses of polyunsaturated acids from the neutral lipids were much smaller, suggesting a selectively protective mechanism or environment in that fraction. The changes in the phospholipid fatty acids may provide the basis for useful objective tests of fish lecomposilion.


2018 ◽  
Vol 20 (11) ◽  
pp. 2661-2669 ◽  
Author(s):  
Sandro Spiller ◽  
Matthias Blüher ◽  
Ralf Hoffmann

2018 ◽  
Vol 2018 ◽  
pp. 1-8
Author(s):  
Alexander M. Wathne ◽  
Hanne Devle ◽  
Carl Fredrik Naess-Andresen ◽  
Dag Ekeberg

Fatty acid (FA) profiles of the species Tettigonia viridissima, Chorthippus biguttulus, and Chorthippus brunneus were determined and quantitated. Extracted lipids were derivatized into FA methyl esters (FAMEs) prior to analysis by GC-MS. A total of 37 different FAs were identified in T. viridissima, yielding a total FA content of 10.4 g/100 g of dry matter. The contents of saturated FAs, monounsaturated FAs, and polyunsaturated FAs were 31.1, 35.9, and 33.0%, respectively. Lipids from T. viridissima were also fractioned into neutral lipids, free fatty acids, and polar lipids by offline solid phase extraction. For C. brunneus and C. biguttulus, 33 FAs were identified, yielding a total FA content of 6.14 g/100 g of dry matter. SFAs, MUFAs, and PUFAs, respectively, constituted 32.7, 25.1, and 42.1% of the total FA content. The contents of MUFAs, PUFAs, n-3 FAs, and n-6 FAs of each species, and the n-6/n-3 ratio, were subsequently discussed.


1989 ◽  
Vol 35 (11) ◽  
pp. 1009-1014 ◽  
Author(s):  
Yoshiko Arimitsu ◽  
Atsuko Moribayashi ◽  
Norihisa Goto

Sonically disrupted cells from avirulent strain Shibaura of Leptospira interrogans serovar copenhageni induced a skin reaction characterized by infiltration of polymorphonuclear leukocytes (PMN) associated with some edema in guinea pigs. To determine the substance inducing infiltration of PMN, lipids of avirulent strain Shibaura were extracted with chloroform – methanol – water after washing with acetone. The lipids comprised 28% of the dry weight of the cell. When the lipids were further separated into water–methanol and chloroform fractions, the most severe PMN infiltration of all samples was seen in the skin inoculated with extract recovered from the chloroform fraction. Neutral and polar lipids were detected after thin-layer chromatography of the chloroform extract. Neutral lipids were detected as free fatty acids (FFA). Fatty acids contained in polar lipids were mainly palmitic acid and palmitoleic acid, whereas FFA comprised 66.5% oleic acid. Skin reactions consisting of marked edema with mild infiltration of PMN were elicited by FFA. There was no obvious difference between a commercially available FFA mixture and the FFA from avirulent strain Shibaura. These observations suggest that FFA may play some role in the pathogenesis of leptospirosis.Key words: Leptospira interrogans serovar copenhageni avirulent strain Shibaura, polar lipids, free fatty acids, skin reaction.


1994 ◽  
Vol 72 (2) ◽  
pp. 113-116 ◽  
Author(s):  
Jacques LeBlanc ◽  
Julie Soucy

The effect of various doses of i.p. injection of the adenosine receptor agonist (R)-phenylisopropyladenosine (R-PIA), ranging from nanomolar to micromolar concentrations, on plasma levels of free fatty acids, glucose, insulin, glucagon, ACTH, and corticosterone was examined in 200-g male rats. At the lowest dose of R-PIA (0.005 μmol/kg), a marked decrease in plasma insulin and free fatty acids was observed. This effect on free fatty acids persisted up to the highest concentration of R-PIA (50 μmol/kg). The insulin response showed a similar pattern except at the highest concentration, when the plasma levels were within normal ranges. A 100% increase in plasma glucose was found, but only with doses of 0.5 μmol/kg and above, suggesting an A2 receptor influence, possibly related to the elevation of plasma glucagon observed with the same doses of R-PIA. It has been shown that caffeine, an antagonist of adenosine, stimulates the pituitary–adrenal axis. Surprisingly, it was shown that R-PIA produces the same effect, as evidenced by the marked elevation of both plasma ACTH and corticosterone at concentrations of 0.5 μmol/kg and higher. It is suggested that this centrally mediated effect is due to a primary peripheral action.Key words: adenosine, glucose, free fatty acids, insulin, glucagon, ACTH, corticosterone.


1987 ◽  
Vol 35 (02) ◽  
pp. 96-100 ◽  
Author(s):  
F. Nilsson ◽  
E. Berglin W-O ◽  
R. Ekroth ◽  
G. Holm ◽  
I. Milocco ◽  
...  

2019 ◽  
Author(s):  
Bela Kikalishvili ◽  
Tsitsana Sulakvelidze ◽  
Nina Vachnadze ◽  
Manana Malania ◽  
Durmishkhan Turabelidze

Crude neutral lipids were obtained from aerial parts of alkaloid-containing plants Chelidoniummayus L, VincaherbaceaWaldts. et.kit, Vinca minor L. Physalisalkekengi L var. franchetii, growing in Georgia and their qualitative composition was established. As well free fatty acids were identified quantitatively and qualitatively using HPLC.


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