scholarly journals IMPROVEMENT OF THE CONTENT OF THE DRIGALSKI LACTOSE AGAR NUTRIENT MEDIUM

Author(s):  
Владимир Ермаков ◽  
Vlyadimir Ermakov ◽  
Оксана Датченко ◽  
Oksana Datchenko ◽  
Юлия Курлыкова ◽  
...  

The purpose of the study is to improve the mix formulation of the Drigalski Lactose Agar breeding ground, produced by AppliChem, and aimed at enterobacteria isolation and differentiation. Development of a new mix formulation for the Drigalski Lactose Agar breeding ground, efficient assessment of cultivation of enterobacteria on its improved base and other breeding grounds used in the laboratory practice by the authors was the task of the study. The im-proved agar Drigalsky differential medium with lactose is recommended for cultivation (isolation) of different enter-obacteria of Enterobacteriaceae family.Differentiation of enterobacteria on improved medium is carried out accord-ing to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The medium can also be used for sanitary and microbiological examination of environmental objects.The medium can be used also to conduct the ONPG test. The efficiency of cultivation of enterobacteria isolated from various animal species on the most frequently used differential diagnostic medium, including new formulation of the Drigalski Lactose Agar, ranged from 16.28 ± 1.44 to 42.18 ± 4.12 hours. Enterobacteria isolated from farm and wild animals formed colo-nies on improved Drygalsky agar with lactose during 24 hours, and enterobacteria isolated from zoo animals within 25-31 hours.

2021 ◽  
Vol 8 ◽  
Author(s):  
Pavel Kvapil ◽  
Joško Račnik ◽  
Marjan Kastelic ◽  
Pavlína Pittermannová ◽  
Tatjana Avšič-Zupanc ◽  
...  

Monitoring infectious diseases is one of the most important pillars of preventative veterinary medicine in zoological collections. The zoo environment offers a great variety of different animal species living in proximity and in contact with small wild animals and vectors (e.g., ticks and mosquitos). In this context, tick-borne encephalitis virus (TBEV), Usutu virus (USUV), and West Nile virus (WNV) causing vector-borne diseases are emerging pathogens that raise concern. The aim of the study was to detect antibodies to selected flaviviruses in various animal species in the Ljubljana Zoo, Slovenia. In total, 874 sera from 96 animal species were tested for antibodies to TBEV by enzyme-linked immunosorbent assays (ELISA); positive samples were confirmed by a virus neutralization test (VNT) using TBEV, WNV, and USUV antigens. Antibodies to TBEV were detected by ELISA in 3.9% (34/874) of zoo animals, with 4% (30/753) in mammals and 5% (4/86) in birds; the sera of reptiles (n = 34) and amphibians (n = 1) were negative. Antibodies to TBEV were confirmed by VNT in 11 mammals; one bird was positive for both WNV and USUV. The mixture of exotic animal species and their contact with wild animals and vectors such as ticks and mosquitos suggest that screening of infectious diseases in zoo animals might provide good insight into the epizootological situation of the area. This is the first survey of TBEV, WNV, and USUV in a zoological collection in Slovenia.


2019 ◽  
pp. 78-91
Author(s):  
Vahid Nasiri ◽  
Farnoosh Jameie ◽  
Habibollah Paykari ◽  
Nahideh Mazhari ◽  
Saba Soltani ◽  
...  

Zoos are places where a great number of valuable animal species are put together taken out of their natural habitats .This survey was carried out to survey the gastrointestinal parasites in animals at a zoological garden in Tehran, Iran. A total of 143 fecal samples from various captive wild animals, consisting of 40 different species were collected randomly and analysed for the presence of the different stages of parasites by direct smear preparation and zinc sulfate flotation followed by Ziel-Neelsen staining method. Data showed that the examined animals were consist of 12 species of carnivores (36 samples), 17 species of herbivores (75), 3 species of non-human primates (11 samples), and 8 species of different species of birds (21 samples). 23(16.08%) of animals, that belonging to 14 animal species, were infected with different intestinal parasites. Among 143 samples from captive wild animals 23 samples (16.08%) were positive for gastrointestinal parasites that 5 samples (3.49%) belong to Carnivores, 13 samples (9.09%) to Herbivores, 4 samples (2.8%) to Non-human primates and 1 sample (0.7%) to Aves. The prevalence of parasites was higher in Non-human primates (36.36%) followed by Herbivores (17.33%), Carnivores (13.88%) and Aves (4.76%).Some animals infected with more than one parasite species and have mixed infection, thus, out of 23 animal samples that parasites were encountered, 12 (8.39%) were infected with helminths and 13 (9.09%) were infected with protozoa. The high prevalence of gastrointestinal parasites found in zoo animals examined in this study emphasizes the importance of controlling these parasitic diseases in order to keep animals, especially in the case of endangered species, in healthy conditions and prevent probable infection of humans working with these animals to zoonotic parasites. Keywords: Wild animals; Zoological park; Parasite, Iran


2020 ◽  
Vol 9 (22) ◽  
Author(s):  
Kasumi Ishida-Kuroki ◽  
Nachiko Takeshita ◽  
Yoshihiro Nitta ◽  
Takehisa Chuma ◽  
Ken Maeda ◽  
...  

ABSTRACT We report 16S rRNA amplicon sequence data from feces from 58 wild boars, 60 feral raccoons, 9 wild Japanese badgers, 21 wild masked palm civets, and 8 wild raccoon dogs in Japan. The predominant bacterial taxa in the fecal microbiota were similar in part but varied among the animal species.


2018 ◽  
Vol VIII (z. 2) ◽  
pp. 123-137
Author(s):  
Magdalena Micińska

Animal traps have always accompanied man, with whom the primary people organized the first hunts. Along with the development of hunting art, traps gradually gave way to specialized hunting weapons. However, the use of animal traps on a large scale still occurs in countries that are world exporters of fur and skins of wild animals - Canada, Russia and the USA. Driven by expressed in art. 13 TFEU with the principle of animal welfare, the European Union has introduced a number of regulations to ensure humane catches in member countries as well as in third countries exporting skin and fur. The purpose of this article is to analyze the current legal situation in Poland with regard to the implementation of EU legislation on humane trap standards, with particular regard to the obligations contained in the agreement concluded between the European Community, Canada and the Russian Federation on 22 July 1997 - on international humane trapping standards . Keywords - EU, Poland, Russia, Canada, USA, animal welfare, humane animal protection, snare, poaching, animal species protection, hunting, animal traps, hunting, trapping, hunting law.


1988 ◽  
Vol 15 (3) ◽  
pp. 239-249 ◽  
Author(s):  
Gbadebo Jonathan Osemeobo

To conserve a resource without having adequate data and finance is difficult and frustrating. The situation of wildlife in Nigeria is nevertheless different. Except in the Yankari, Upper Ogun, and Kwiabaha, Game Reserves, and the Kainji Lake National Park, little efforts have been made to protect the Nigerian animal wildlife resources from human pressure and widespread extinction. To many, what remains of the wild animals are best seen in the few state-owned zoological gardens in Nigeria. However, because most indigenous large animal species—including Elephant, Buffalo, Chimpanzee, Gorilla, Rhinoceros, Leopard, and Ostrich—have not been able to reproduce in the various zoological gardens so far, the hope to conserve these animals is brittle. The questions are, what factors are working against wildlife conservation? Indeed, what hopes exist for Nigerian animal wildlife?Animal wildlife is a declining resource in Nigeria because of unplanned land-use practices. For example, landuses in game reserves are often conflicting and contradictory. Four land-uses: timber extraction, hunting, food-crop production, and settlement, are simultaneously going on in game reserves, with little or no control measures and with no management plans. The excessive demands for land for these conflicting uses have greatly disturbed the ecosystems involved, thus making the survival of the wild animals uncertain. Specifically, the problems of wildlife conservation in Nigeria are: (a) poaching; (b) indiscriminate burning of the vegetation; (c) uncontrolled grazing activities in the reserves; (d) intensive logging for domestic and industrial uses; (e) user rights on the reserves enjoyed by the traditional owners of the land before reservation; (f) lack of adequate funds to manage the reserves; (g) ineffective legislation; (h) lack of trained manpower; (i) urban sprawl; and (j) infrastructural development of roads, electric and telegraph lines, and irrigation schemes, all within the game reserves.The future for Nigerian animal wildlife depends on the nation's ability to conserve what is left either in their natural habitat or, at least, in zoological gardens. The task is not simple under conditions of economic depression, with inadequate manpower and without effective management of game reserves. In these circumstances, the game reserves should be reduced to manageable numbers, while state governments should win public sympathy through adequate conservation publicity and the provision of sufficient vehicles and personnel to manage the game reserves.The policy of land-use in game reserves should be reviewed, while researches should be conducted on (a) the levels of land-use that could be consistent with maintaining wild animals in the reserves, (b) the number and species of animals hunted per year, (c) the population of animal species in the game reserves and their habitat suitability, (d) the endangered and extinct animal species and specific reasons for the decline in their populations, and (e) human problems peculiar to each reserve and ways of minimizing them.


2020 ◽  
Vol 48 ◽  
Author(s):  
Marco Aurélio Cunha Del Vechio ◽  
Edson Gerônimo ◽  
Enzo Emmerich Paula De Castro ◽  
Luciana Vieira Pinto Ribeiro ◽  
Rita De Cássia Lima Ribeiro ◽  
...  

Background: Studies on intestinal parasites in cougars Puma concolor are scarce, and most of the available information on the species has been derived from individuals in captivity. One of the greatest threats to the survival of wild cats is habitat loss caused by urban sprawl, agricultural matrix, and linear developments such as highways and railways, which expose animals to direct contact with humans and to a high risk of death. Given the lack of scientific data the objective of this study was to report on the occurrence of intestinal parasites in a cougar (P. concolor) specimen from the northwestern region of Paraná State, Brazil.Case: The carcass of a free-living cougar (P. concolor) individual was sent to the Interdisciplinary Science Museum (Museu Interdisciplinar de Ciências – MIC) of Paranaense University (Universidade Paranaense - UNIPAR); an individual was killed during a collision with an unidentified vehicle on highway PR-486, in the municipality of Mariluz (PR, Brazil). The geographical coordinates of the location where the animal was found are: 23°59′29″S, 53°8′47″W. This region is characterized by semi-deciduous seasonal forest remnants. After being identified on site and collected by the inspectors of the Paraná Environmental Institute (Instituto Ambiental do Paraná - IAP), the cadaver was donated to the MIC (by the IAP) for a necropsy to elucidate the cause of death. In this context, the cadaver was sent to the Animal Pathology section of the Department of Veterinary Medicine at UNIPAR. The animal was necropsied by performing the standard necropsy technique for carnivores: opening the intestine through the mesentery and exposing the mucosa from the duodenum to the colon. The necropsy showed that the cause of death was hypovolemic shock due to trauma. During necropsy, parasites in the intestinal lumen were visible to the naked eye. Parasites and feces were collected and stored in sterile flasks containing 10% formaldehyde and saline. The parasites were subjected to Faust and Hoffman techniques, micrometry, and morphological analysis, resulting in the identification of the roundworm Toxocara cati and the tapeworm Spirometra decipiens.Discussion: Identifying the roadkill species along highways is of paramount importance, since they are living very close to man and, consequently, reservoirs and disseminators of different infectious and parasitic zoonoses may be possible. Infection by protozoa and helminths in animals usually occurs by ingesting the infective form present in food or water. Toxocara cati and the tapeworm Spirometra decipiens were only reported in captivity before. Research on parasites in free-living wild animals is not common in the scientific literature and this is due to the difficulty of capturing this animal category as they live in difficult to access places such as forests and forests, therefore, research projects in partnership with museums are extremely important for the recognition of wild animal species that circulate in the region, in addition to the parasitic identification of these animals for future sanitary measures and for the preservation of the animal species in the environment. The Parasitological studies of wild animals are highly relevant for expanding this knowledge, especially considering the possible transfer risk of specific parasitic diseases to other animals, as well as to humans.


2017 ◽  
Vol 73 (7) ◽  
pp. 387-394
Author(s):  
Agnieszka Kaupke ◽  
Artur Rzeżutka

Cryptosporidium parvum is a zoonotic protozoan parasite occurring in a wide range of hosts. Invasions caused by this parasite have been reported in humans and in many animal species including birds. Despite its worldwide prevalence, infections have usually generated considerable losses in the livestock industry, mostly affecting calves, lambs and goat kids. It has previously been shown that ruminants are a major reservoir of zoonotic Cryptosporidium parvum and contact with an infected animal can lead to human infection. The application of molecular methods for parasitological diagnostics has increased our knowledge on the parasite hosts and its prevalence in humans and animals. They also confirmed their usefulness during epidemiological investigations and in surveillance studies of human and animal cryptosporidiosis. In this review the current state of knowledge concerning the importance of Cryptosporidium parvum invasions in farm and wild animals was presented.


Author(s):  
João PGLM Rodrigues ◽  
Susana Barrera-Vilarmau ◽  
João MC Teixeira ◽  
Elizabeth Seckel ◽  
Panagiotis Kastritis ◽  
...  

AbstractSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is responsible for the ongoing global pandemic that has infected more than 14 million people in more than 180 countries worldwide. Like other coronaviruses, SARS-CoV-2 is thought to have been transmitted to humans from wild animals. Given the scale and widespread geographical distribution of the current pandemic, the question emerges whether human-to-animal transmission is possible and if so, which animal species are most at risk. Here, we investigated the structural properties of several ACE2 orthologs bound to the SARS-CoV-2 spike protein. We found that species known not to be susceptible to SARS-CoV-2 infection have non-conservative mutations in several ACE2 amino acid residues that disrupt key polar and charged contacts with the viral spike protein. Our models also predict affinity-enhancing mutations that could be used to design ACE2 variants for therapeutic purposes. Finally, our study provides a blueprint for modeling viral-host protein interactions and highlights several important considerations when designing these computational studies and analyzing their results.


The Auk ◽  
2019 ◽  
Vol 136 (1) ◽  
Author(s):  
Kara A Navock ◽  
David H Johnson ◽  
Samantha Evans ◽  
Matthew J Kohn ◽  
James R Belthoff

ABSTRACT Host-parasite relationships between Western Burrowing Owls (Athene cunicularia hypugaea) and the fleas (Pulex irritans, Siphonaptera:Pulicidae) they harbor were studied to understand the extent to which migratory Burrowing Owls translocated fleas from wintering grounds to breeding grounds. This has implications for host-parasite relationships in Burrowing Owls and also potentially for the dynamics of plague, as Burrowing Owl distributions overlap plague foci, owls inhabit fossorial mammal colonies where epizootic outbreaks of plague occur, and owls may harbor species of flea that are competent plague vectors. We used hydrogen stable isotope analysis to help elucidate geographic origins of fleas collected from adults and nestlings in 2 migratory populations of Burrowing Owls in Idaho and Oregon, USA. For adults, we posited that bird-mediated dispersal would impart flea isotopic compositions representative of southern latitudes and be similar to owl toenail tissue recently grown on wintering grounds, but they would differ from contour feathers presumably grown on breeding grounds the previous year. We assumed nestling feathers and toenails would have isotopic compositions representative of the breeding grounds. We analyzed contour feathers and toenails from adults collected shortly after they arrived in breeding grounds following spring migration and from nestlings later in the breeding season, to which we compared isotopic compositions in fleas collected from individuals of both age classes. Fleas on nestlings in both populations had isotopic compositions that did not differ from nestling feathers and toenails, suggesting that nestling fleas had breeding ground origins. Fleas on adults in one population (Oregon) had breeding ground isotopic signatures, as flea compositions did not differ from nestling feathers or toenails. Adult owls in Idaho had fleas that similarly did not express a wintering ground signature, but they were enriched in the heavy isotope (deuterium) relative to nestling feathers and toenails. Therefore, we discuss the possibility that adult owls in Idaho acquired fleas at migratory stopover sites. While the latter indicates that Burrowing Owls have the potential to disperse fleas, there was no evidence of continent-wide movement of fleas by owls from wintering grounds to breeding grounds.


2016 ◽  
Vol 39 (2) ◽  
pp. 97-100
Author(s):  
Chandra Kala

The Himalayan grasslands from sub-tropical to temperate, alpine and steppe have been exploited historically by humans for multiple uses including grazing lands for livestock. Besides domestic and wild animals, a large number of migratory birds use these grasslands and wetland areas dotted in these grasslands during their vertical and horizontal migration in the Himalayan region during breeding season. Despite quite unique and sensitive ecosystems these grasslands are least studied. The present study, therefore, aims to study the structure and composition of Himalayan grasslands with respect to their multiple utilities, including breeding grounds for migratory birds.


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