scholarly journals THE PECULIARITIES LOCAL IMMUNE REACTIONS IN ILEUM AT POSTRESECTION PORTAL HYPERTENSION

2018 ◽  
Vol 17 (2) ◽  
pp. 71-75
Author(s):  
L. V. Tatarchuk ◽  
M. S. Hnatyuk
Keyword(s):  
Portal Hypertension ◽  
Mast Cells ◽  
Cell Density ◽  
Structural Changes ◽  
White Male ◽  
Liver Parenchyma ◽  
Male Rats ◽  
Secretory Iga ◽  
Immune Homeostasis ◽  
Immune Reactions

In the experiment, in laboratory white male rats, local immune reactions were investigated in the ileum in conditions of postresection portal hypertension, which was modeled by removing 58.1% of liver parenchyma. It has been established that postresection portal hypertension leads to pronounced changes in local immune reactions in the ileum, characterized by a 29.6% reduction in secretory IgA, a plasmoncytes with IgA on 1 mm² on the mucosa by 32.5%, an uneven, disproportional increase in the number of plasmatic cells with IgM. IgG, IgE, respectively, by 77.0%, 4.1 and 5.1 times. The cell density of the infiltrate in the mucosa of the investigated organ increased by 37.0%, and the number of mast cells by 35.8%. The results obtained indicate that postresection portal hypertension leads to weakening, tension and instability of the local immune homeostasis of the ileum mucosa and marked structural changes in the wall of the investigated organ.

scholarly journals Morphometric evaluation of features of remodeling of chondrocites of joint surfaces of temporomandibular joint of experimental animals in hyperglycemia

2021 ◽  
Vol 11 (6) ◽  
pp. 137-142
Author(s):  
Mykhailo Hnatiuk ◽  
Lesia Rubas
Keyword(s):  
Temporomandibular Joint ◽  
Structural Changes ◽  
Articular Surface ◽  
White Male ◽  
Relative Volume ◽  
Male Rats ◽  
Intercellular Substance ◽  
Temporal Fossa ◽  
Articular Surfaces ◽  
Joint Surfaces

Aim: to study morphometrically the peculiarities of remodeling of chondrocytes of the articular surfaces of the temporomandibular joint in hyperglycemia.Materials and methods: Quantitative morphological methods were used to study diameters of chondrocytes and their nuclei, nuclear-cytoplasmic relationship in these cells, relative volume of damaged chondrocytes, intercellular-chondrocytic relationship in mature chondrocytes of the articular surfaces of the temporomandibular joint of 45 laboratory mature white male rats with simulated diabetes.Results: A comprehensive analysis of the morphometric parameters presented in the article showed that they did not differ in the articular surfaces of the temporal fossa and mandibular head of the intact temporomandibular joint. The expressed structural changes of chondrocytes were revealed at a monthly and a two-month diabetic arthropathy of this joint.Conclusions: Proceeded studies and obtained results suggest that prolonged hyperglycemia leads to diabetic arthropathy of the TMJ, which is characterized by atrophy of chondrocytes, violation of nuclear-cytoplasmic relationships, an increase in the relative volume of damaged chondrocytes and volume of intercellular substance. The detected changes in quantitative morphological parameters depended on the duration of hyperglycemia and dominated in the cartilaginous tissue of the articular surface of the mandibular head.

scholarly journals MORPHOMETRIC ANALYSIS REMODELING VESSELS HEMOMICROCIRCULATORY BED OF JEJUNUM AT RESECTIONS OF LIVER

2018 ◽  
Vol 24 (1) ◽  
pp. 16-20
Author(s):  
M.S. Hnatjuk ◽  
L.V. Tatarchuk
Keyword(s):  
Portal Hypertension ◽  
Varicose Veins ◽  
Liver Parenchyma ◽  
Male Rats ◽  
Lateral Part ◽  
The Difference ◽  
The Right ◽  
Bed Expansion ◽  
Quantitative Indicators ◽  
Sexually Mature

Liver resection is widely used in modern surgical departments. Removal of large volumes of the liver leads to postresection portal hypertension, which is complicated by bleeding from varicose veins of the esophagus, stomach, rectum, ascites, splenomegaly with secondary hypersplenism, parenchymal jaundice and portosystemic encephalopathy. The widespread prevalence of this pathology, high mortality from its complications indicates that it is an important medical and social problem. The aim of the study is quantitative morphological study of the features of remodeling the vessels of the hemomicrocirculatory bad of the jejunum at resection of different volumes of the liver. The studies were conducted on 45 sexually mature male rats, which were divided into 3 groups. The first group consisted of 15 intact virtually animals, 2-15 rats after resection of the left lateral part – 31.5% of the parenchyma of the liver, 3-15 animals after resection of the right and left lateral parts of the liver (58.1%). Euthanasia of experimental animals was carried out by bloodletting in conditions of thiopental anesthesia 1 month after the beginning of the experiment. The hemomicrocirculatory bad of the jejunum was studied by injection of its vessels into the carcass-gelatinous mixture, which was injected through the abdominal aorta. From the jejunum, lightened and histologic preparates were prepared and morphometrically studied the vessels of the microhemocirculatory bed. Quantitative indicators were processed statistically, the difference between the comparative values was determined by the Student's criterion. It was established that the severity of structural transformation of the vessels of the microhemocirculatory bad of the jejunum depends on the volume of the removed parenchyma of the liver. The analysis of the obtained results revealed that one month after resection of 31.5% of liver parenchyma, the morphometric parameters of the vessels of the haemomicrocirculatory bad of the jejunum were insignificantly altered. It was determined that resection of 58.1% of liver parenchyma caused postresection portal hypertension and marked remodeling of blood vessels of the hemomicrocirculatory bad of the jejunum, which was characterized by narrowing of arterioles by 23.4%, precapillary arterioles by 22.8%, hemocapillaries by 22.9%%, decrease in the density of microvessels – by 22.4%, the expansion of the capillary venules – by 35.5% and venules – by 28.7%, venous plethora, hypoxia, dystrophy, necrobiosis of cells and tissues, infiltrative processes and sclerosis. Resection of 58.1% of liver parenchyma leads to postresection portal hypertension, pronounced narrowing of lumen of the vessels of the adnexa and exchange units hemomicrocirculatory bed, expansion of the postcapillary venules and venules, venous plethora, hypoxia, dystrophy and necrobiosis of cells and tissues, infiltrative and sclerotic processes in wall of the jejunum.

scholarly journals Стан антиоксидантної системи експериментальних тварин при пошкодженні дванадцятипалої кишки за умов пострезекційної портальної гіпертензії

2018 ◽  
Author(s):  
M. S. Hnatjuk ◽  
L. V. Tatarchuk
Keyword(s):  
Portal Hypertension ◽  
Epithelial Cells ◽  
Antioxidant System ◽  
Liver Parenchyma ◽  
The State ◽  
Male Rats ◽  
Antioxidant Defence ◽  
Experimental Animals ◽  
Functional Changes ◽  
Smooth Myocytes

Introduction. The resections of large volumes of the liver leads to postresection portal hypertension that results in structural-functional changes in the organs of portal vena system. The features of remodeling structures of duodenum and state of the antioxidant system of organism in the conditions of postresection portal hypertension are not studied yet. The aim of the study – to learn the state of the antioxidant system for experimental animals at the structural-functional changes of duodenum in the conditions of postresection portal hypertension. Research Methods. The studies were performed on 39 white male rats, which were divided into 3 groups. The first group consisted of 15 intact experimental animals (control), 2nd – 12 animals, which eliminated 42 % of liver parenchyma, 3rd – 12 rats after resection of 58.1 % of liver parenchyma. One month after the beginning of the experiment, euthanasia of rats was performed by bloodletting under conditions of thiopental anesthesia. In the blood of experimental animals, indicators characterizing the state of oxidative protection were determined – the activity of superoxidedismutase, catalase and ceruloplasmin. Sections of the duodenum were fixed in a 10 % neutral formalin solution and, after passing through the ethyl alcohol of increasing concentration, were placed in paraffin. Histologic sections 5–7 мm thick after deparaffinization were stained with hematoxylin-eosin, for van Gizon, Mallory, Weigert, and toluidine blue. Gistostereometrically determined the relative volumes of damaged endothelial cells, epithelial cells, smooth myocytes. A correlation analysis was carried out between biochemical and histogeometric indices with the definition of the coefficient (r) of correlation. Quantitative values were processed statistically. Results and Discussion. It is set that in the conditions of the modelled experiment activity of superoxidedismutase at the resection of 42 % parenchyma of liver statistically for certain (р<0.001) diminished on 15.5 % and at removal of 58.1 % parenchima of liver – on 25 %; activity of catalase – accordingly on 22.2 and 38.8 % and activity of ceruloplasmin – on 15.3 and 21.3 %. Received and the analyzed indexes testify that at resection of considerable volumes (42; 58.1 %) parenchyma of liver antioxidant defence of organism gets worse substantially and depends on removal at volumes of liver. A relative volume of the damaged endotheliocytes is in 2 to the group of supervisions with high authenticity (р<0.001) grew in 8.4 times, epithelial cells – in 18.9 times, and smooth myocytes – in 12 times. After the resection of 58.1 % parenchyma of liver the investigated morphometric parameters increased accordingly in 15.8; 31.4 and 18.1 times. Correlation connections between the indexes of antioxidant defence and relative at volumes of the damaged endotheliocytes, epithelial cells and smooth myocytes in duodenum at resection 42 % parenchima of liver were opposite moderate and significance (r=–0.48ч–0.56) at resection 58.1 % of liver – significance (r=–0.72÷-0.83). Conclusions. The obtained results indicate that antioxidant protection of the organism plays an important role in the adaptive-compensatory processes of the duodenum after resection of different volumes of liver parenchyma. The degree of antioxidant defense of the organism depends on the removed volume of liver parenchyma and the number of damaged structures of the duodenum.

scholarly journals INFLUENCE OF COMBINED INJECTION OF THYROXIN AND PROPYLTHIOURACILUM ON SCTRUCTURAL INDICATORS OF RENAL PARENCHYMA

2017 ◽  
Vol 21 (1) ◽  
pp. 57-67
Author(s):  
S. I. Dolomatov ◽  
V. G. Sipovski ◽  
N. Y. Novikov ◽  
I. N. Kasich ◽  
I. V. Myshko ◽  
...  
Keyword(s):  
Body Weight ◽  
Structural Changes ◽  
White Male ◽  
Kidney Tissue ◽  
Renal Parenchyma ◽  
Male Rats ◽  
Intragastric Administration ◽  
Tissue Samples ◽  
Hematoxylin And Eosin

THE AIM: to study of the dynamics of structural changes in renal parenchyma of rats exposed to long-term combined effects of thyroxine and propylthiouracilum (PTU). MATHERIAL AND METHODS – studies were performed on mongrel white male rats weighing 250-300g. Hyperthyroidism was caused by daily intragastric administration of thyroxine (T4) in amount of 50g per 100g of body weight over 30 days. On the first day of the experiment animals were divided into 2 groups. Animals of the first group (n = 25) received only T4. The rats of the second group (n = 25) were administrated propylthiouracilum and T4 daily. PTU was administered intragastric in amount of 1 mg per 100g of body weight. Kidney tissue samples were collected on the 10th, 20th and 30th days of the experiment. In addition, there were collected kidney tissue samples of the animals treated with only T4 after 20 days after cessation of hormone. Obtained tissue samples were fixed and treated by the usual method, followed by filling in paraffin. Sections were stained with hematoxylin and eosin. RESULTS – it was established that course of experimental hyperthyroidism leads to significant structural abnormalities of the renal parenchyma. Leading features of kidneys pathology at a hyperthyroidism are rough structural damages of the nephron tubular epithelium. CONCLUSIONS – combined administration in rats of thyroxin and propylthiouracilum has weakly expressed beneficial effect by limiting the development of structural damages to the renal parenchyma and clot formation. 

scholarly journals Morphometric aspects of studying the features of remodeling of atrial structures at postresection portal hypertension

2021 ◽  
Vol 11 (11) ◽  
pp. 214-220
Author(s):  
N. Gdanska ◽  
M. Hnatjuk ◽  
L. Tatarchuk ◽  
N. Monastyrska
Keyword(s):  
Portal Hypertension ◽  
Left Atrium ◽  
Morphological Changes ◽  
Multiorgan Failure ◽  
White Male ◽  
Male Rats ◽  
White Rats ◽  
Left And Right ◽  
Structural Homeostasis ◽  
Right Atria

Introduction. Removal of the left and right lateral lobes of the liver in white rats leads to postresection portal hypertension, which is characterized by dilation and plethora of the hepatic portal vein, mesenteric veins, esophageal and gastric veins, veins of the anterior abdominal wall, splenomegaly, ascites and multiorgan failure. Objective of the research: to study the features of remodeling of the structures of the atria at postresection portal hypertension histostereometrically. Materials of the research and their discussion. The peculiarities of remodeling the structures of the atria were histostoreometrically studied in 65 adult white male rats, which were divided into 3 groups: the 1- consisted of 20 intact animals, the 2 – 30 rats with postresection portal hypertension, which was modeled by removal of the left and right lateral lobes of the liver, the 3 – 15 animals with postresection portal hypertension and multiorgan failure. One month after the start of the experiment, rats were euthanized by bloodletting under conditions of thiopental anesthesia. Histological micropreparations were used to determine the diameters of cardiomyocytes of the left and right atria, diameters of their nuclei, nuclear-cytoplasmic relations, relative volumes of cardiomyocytes, capillaries, stroma, stromal-cardiomyocyte, capillary-cardiomyocyte relations, relative volumes of damaged cardiomyocytes. Quantitative indicators were processed statistically. Results of the research and their discussion. Histostereometrically, it was found that postresection portal hypertrophy leads to hypertrophy of atrial cardiomyocytes, their nuclei, growth of stromal structures, relative volumes of damaged cardiomyocytes, disorders of tissue and cellular structural homeostasis. The revealed features of remodeling of the studied structures dominated in the left atrium at combination of postresection portal hypertension with multiorgan failure. Conclusions. Postresection portal hypertension leads to pronounced remodeling of the structures of the left and right atria, characterized by hypertrophy of cardiomyocytes, changes in their nuclear-cytoplasmic relations, an increase of the relative volume of stroma, damaged cardiomyocytes, pronounced disorders of tissue and cellular structural  homeostasis. The revealed morphological changes dominated in the left atrium at combination of postresection portal hypertension with multiorgan failure.

scholarly journals Peculiarities of structural reorganization of the thyroid gland vessels in dynamics after experimental thermal trauma

2018 ◽  
Vol 22 (4) ◽  
pp. 610-615
Author(s):  
V.G. Korytskyi
Keyword(s):  
Thyroid Gland ◽  
Structural Changes ◽  
Burn Injury ◽  
White Male ◽  
The Body ◽  
Male Rats ◽  
Time Interval ◽  
Thermal Trauma ◽  
Vascular Bed ◽  
Ketamine Anesthesia

Thermal trauma and burn disease, which develops from deep, large lesions, are accompanied by significant morphological and functional changes not only in the skin, but also in organs of all systems of the body, including thyroid gland. The purpose of the study was to establish a microscopic reorganization of the animal thyroid gland in dynamics after an experimental thermal trauma. Burns of the third degree were applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97–100 °С. The affected area corresponded 18–20% of the surface of the rat’s body. Experimental study of structural changes in the vascular bed of the thyroid gland after severe thermal trauma was carried out on laboratory white male rats weighing 160–180 g. Euthanasia of rats was performed after ketamine anesthesia, by decapitation. Structural changes in the thyroid gland were studied after 1, 7, 14 and 21 days from the beginning of the experiment. Histologic sections of 5–6 µm thickness were stained with hematoxylin-eosin. For the study of the connective tissue condition and its main substance, silver impregnation was performed by using the Gordon-Sweet's method (to detect reticular and immature collagen fibers), staining with 3-colored MSB — selective coloring method for intravascular coagulation syndrome. It has been established that in the early stages after the thermal trauma (1–7 days of the experiment, the stage of shock and early toxemia) there are adaptive-compensatory changes and initial signs of destruction of the vascular bed structural components of the organ. In late terms (14–21 days of the experiment, the stages of late toxemia and septicotoxemia) significant destructive-degenerative changes of the thyroid gland arteries, veins and blood vessels of the hemocirculatory bed were detected. Therefore, a severe burn injury causes deep histological modification of the thyroid gland vessels, the degree of intensity of which depends on the time interval of the experiment, which adversely affects on the functioning of the organ and transvascular metabolic processes. In further researches it is planned to study the course of morphological changes of thyroid gland vessels during thermal trauma with combined application of corrective methods.  

scholarly journals MORPHOMETRIC EVALUATION OF SPERMATOGENIC EPITHELIOCYTES STRUCTURE UNDER THE RUBOMYCIN INFLUENCE IN RATS

2020 ◽  
Vol 6 (1) ◽  
pp. 68-73
Author(s):  
M. S. Hnatiuk ◽  
S. О. Konovalenko ◽  
L. V. Tatarchuk ◽  
О. B. Yasinovsky
Keyword(s):  
Epithelial Cells ◽  
Structural Changes ◽  
White Male ◽  
Relative Volume ◽  
Male Rats ◽  
Morphometric Parameter ◽  
Testicular Damage ◽  
Experimental Conditions ◽  
Significant Difference ◽  
Spermatogenic Epithelium

Background. Over the last 20 years, the quality of men’s sperm around the world has deteriorated twice. The causes are poor environment, stress, sedentary lifestyle, prevalence of sexually transmitted infections, obesity, alcohol abuse and various stimulants. The structure and function of the testes, when exposed to various drugs and chemicals, attracts the attention of present researchers. Objectives. The aim of the research was to study the structural changes of the spermatogenic epithelium in testicular damage caused by rubomycin hydrochloride. Methods. The spermatogenic epithelium of the testes of 30 white adult white male rats, which were divided into 2 groups, was morphometrically examined. The 1st group comprised 15 experimental intact animals, the 2nd – 15 rats, in which testicular damage with rubomycin hydrochloride was simulated. Euthanasia of rats was performed by bloodletting under thiopental anesthesia one month after the experiment beginning. Quantitative morphological parameters were statistically processed. In spermatocytes of the 1st P-order, spermatogonia and spermatids of testes, their height, diameter of nuclei, nuclear-cytoplasmic ratio and relative volumes of damaged cells of spermatogenic epithelium were evaluated. Results. It was established that spermatogenic epithelial cells were damaged in the simulated experimental conditions, which was morphometrically confirmed by changes in nuclear-cytoplasmic relations in the 1st, 2nd order spermatocytes, spermatogonia and spermatids. The relative volumes of damaged spermatogenic epithelial cells also increased significantly. The most significant morphometric parameters were altered in spermatids. Thus, the nuclear-cytoplasmic ratio in the studied cells was statistically significantly (p <0.001) increased by 7.4% compared with the similar control morphometric parameter. The relative volume of damaged spermatids in these experimental conditions reached (32.50±0.18) %, which with a high statistically significant difference (p<0.001) exceeded the same control value in almost 15.8 times. Optically, histological preparations of the testes showed severe vascular disorders, characterized by dilation and plethora mostly of venous vessels, which pointed to violation of venous drainage of the studied organs. Conclusions. According to the attained research results it has been established that nuclear-cytoplasmic relations are an objective and valuable informative indicator of a functional condition of cells and their structural changes in pathological conditions. When rubomycin hydrochloride is exposed to experimental animals, the most significant changes in nuclear-cytoplasmic relations are found in spermatic spermatozoa.

scholarly journals Peculiarities remodeling the venous bed in the testis at postresection arterial hypertension in small blood circulation

2020 ◽  
Vol 19 (1) ◽  
pp. 20-24
Author(s):  
M. S. Hnatjuk ◽  
S. O. Konovalenko ◽  
L. V. Tatarchuk
Keyword(s):  
Endothelial Cells ◽  
Pulmonary Arterial Hypertension ◽  
Arterial Hypertension ◽  
Structural Changes ◽  
White Male ◽  
Cor Pulmonale ◽  
Male Rats ◽  
Outer Diameter ◽  
External Diameter ◽  
Pulmonary Arterial

Morphological methods examined the venous bad testis of 45 laboratory white male rats, which were divided into 3 groups. The 1 group included 15 intact animals, 2 – 20 rats with pulmonary arterial hypertension and compensated cor pulmonale, 3 – 10 animals with pulmonary arterial hypertension and decompensated cor pulmonale. Hypertension in the small circulatory system and cor pulmonale was modeled by performing right-sided pulmonectomy in animals. It was found that the diameter of the capillary venules of the testis with compensated cor pulmonale was statistically (p<0.01) significantly increased by 4.5 %, and the diameter of venules - by 2.3 % (p<0.05), the external diameter of the venous vessels was statistically significant (p<0.05) increased by 3.1%, and internal – by 2.8 % (p<0.05). The height of endothelial cells of the venous vessels, the diameters of their nuclei in conditions of compensated cor pulmonale did not change significantly. The nuclear-cytoplasmic relations in endothelial cells were unchanged, which testified to the stability of cellular structural homeostasis. Studies and results show that the investigated morphometric parameters of venous vessels in decompensated cor pulmonale changed more pronounced. The diameter of the capillary venules in these experimental conditions increased by 19.1 % (p<0.001), the diameter of the venules – by 22.2 % (p<0.001), the outer diameter of the venous vessels increased by 18.5 % (p<0.001), and inner diameter by 22.3 % (p<0.001). The revealed remodeling of venous vessels resulted in thinning of their walls. At decompensation of the cor pulmonale, the height of the endothelial cells of the venous vessels of the testis decreased by 5.8 % (p<0.01), the diameters of the nuclei of these cells – by 2.8 % (p<0.01). Nuclear-cytoplasmic ratios changed by 6.4 % (p<0.001), indicating a marked disruption of structural cellular homeostasis. The relative volume of damaged endothelial cells of the venous testicular vessels in decompensated cor pulmonale increased in 17.5- times (p<0.001), which could lead to endothelial dysfunction and affect the degree of remodeling venous vessels of the testis. The data obtained indicate that postresection arterial hypertension in the small circle of circulation leads to pronounced structural changes in the venous bad in the testis, which significantly impairs the drainage of venous blood from the specified organ, worsens its trophic supply and plays an important role in its pathomorphogenesis. The most pronounced degree of remodeling of venous vessels was found in decompensated of the cor pulmonale.

scholarly journals ПОРУШЕННЯ ВСМОКТУВАЛЬНОЇ ФУНКЦІЇ ТОНКОЇ КИШКИ ПРИ ПОСТРЕЗЕКЦІЙНІЙ ПОРТАЛЬНІЙ ГІПЕРТЕНЗІЇ

2018 ◽  
Author(s):  
M. S. Hnatiuk ◽  
L. V. Tatarchuk
Keyword(s):  
Small Intestine ◽  
Portal Hypertension ◽  
Epithelial Cells ◽  
Liver Parenchyma ◽  
Relative Volume ◽  
Male Rats ◽  
Morphometric Parameter ◽  
Lateral Part ◽  
Chemical Agent ◽  
Absorption Function

The liver resection is widely used in modern surgical clinics. Removal of large volumes of liver leads to postresection portal hypertension, which is complicated by bleeding from varicose veins of the esophagus, stomach, rectum, ascites, splenomegaly, secondary hypersplenism, parenchymal jaundice, and portosystemic encephalopathy. The widespread prevalence of this pathology, high mortality from its complications indicates that it is an important medical and social problem.The aim of the study – to learn the features of the suction function of the small intestine at postresection portal hypertension.Materials and Methods. The research was conducted on 45 laboratory-sexually mature white male rats, which were divided into 3 groups. The 1 group consisted of 15 intact virtually healthy animals, 2–15 rats after resection of the left lateral part – 31.5 % of liver parenchyma, 3–15 animals after removal of the right and left lateral parts of the liver (58.1 %). One month after the start of the experiment, the suction function of the small intestine was investigated for the D-xylose test. It is specified chemical agent is an inert carbohydrate which is absorbed by passive diffusion. D-xylose is not metabolized in the human body and animals, is excreted in the urine, fully and adequately reflects the suction function of the small intestine. From the small intestine made histological micropreparations. Morphometrically determined the height of the epitheliocytes of the mucousa of the intestine, the diameter of their nuclei, nuclearcytoplasmic relations in them, the relative volume of their damage. Quantitative indicators were processed statistically.Results and Discussion. It was found that one month after resection of 31.5 % of liver parenchyma, the content of D-xylose in urine decreased by only 5.1 %, indicating a slight disruption of the absorption function of the small intestine, and resection 58.1 % of the liver decreased of D-xylose in urine by 28.6 %. The latter figure testified to a marked violation of the suction function of the small intestine. One month after resection, 58.1 % of liver parenchyma decreased the height of epithelial cells by 19.4 % compared with the same control quantitative morphological parameters, and the diameter of their nuclei – only by 2.3 %. Non-uniform disproportionate changes in spatial characteristics of the nucleus and cytoplasm of the epithelial cells resulted in marked violations of nuclear-cytoplasmic relations in these cells. At the same time, the indicated morphometric parameter is statistically significantly (p <0.001) increased by 47.9 %, which indicated an alteration of structural cellular homeostasis. Relative volume of damaged endothelial cells a month after removal of 58.1 % of liver parenchyma increased 21.3 times. Significant correlation between the concentration of D-xylose in urine, nuclear-cytoplasmic ratios in epitheliocytes and the relative volume of damaged epithelial cells was found, which allowed to confirm the close relationship between structural changes in mucosa of the intestine and violation of the suction function in the simulated experimental conditions. Consequently, significant resection of the liver parenchyma leads to posresection portal hypertension, severe lesions of the mucosa of the intestine, which significantly impairs the suction function of the small intestine.Conclusions. Resection of 58.1 % of liver parenchyma leads to postresection portal hypertension and severe structural rearrangement of the epitheliocytes of the mucosa of the small intestine, which is confirmed by a disturbance in their cellular structural homeostasis and a significant increase in the relative volume of their damage, which was complicated by a significant violation of the suction function of the small intestine.

scholarly journals STRUCTURAL CHANGES OF LONG TUBULAR BONES OF MATURE RATS UNDER THE HYPERGLYCEMIA

2021 ◽  
Vol 74 (9) ◽  
pp. 2052-2059
Author(s):  
Alina O. Ponyrko ◽  
Valentyna I. Bumeister ◽  
Serhii M. Dmytruk ◽  
Olha S. Yarmolenko ◽  
Tetiana P. Teslyk ◽  
...  
Keyword(s):  
Sodium Chloride ◽  
Structural Changes ◽  
Morphological Changes ◽  
White Male ◽  
Male Rats ◽  
Control Group ◽  
Cross Sectional ◽  
Left Femur ◽  
Increased Risk ◽  
Tubular Bones

The aim: To evaluate morphological changes in long tubular bones of mature rats under the influence of experimental hyperglycemia. Materials and methods: The study was conducted on 140 nonlinear white male rats divided into two groups. The experimental group included rats that were introduced into a state of hyperglycemia by a single intraperitoneal injection of an alloxan dihydrate solution at a dose of 150 mg / kg body weight in 0.9% sodium chloride. The control group included rats that were injected with a similar volume of 0.9% sodium chloride one time intraperitoneally. The animals were taken out of the experiment on the 2nd, 30th, 60th, 90th, 120th, 150th and 180th day. Right and left femur and humerus were studied by morphometric and histological methods. Results: Under conditions of prolonged uncontrolled hyperglycemia in mature rats, there is a slowdown in the growth rate of length and thickness of femur and humerus. This is indicated by a significant decrease in the length of bone and its diaphyses, as well as by a decrease in the cross-sectional area of the diaphysis, the width of the proximal and distal epiphyses, starting from 120 and 90 days of the experiment, respectively. The relative area of trabecular tissue, thickness of trabeculae and epiphyseal cartilage decreases in comparison with animals of the control group. The diameter of osteons and their channels increases in cortical tissue. Changes in the microarchitecture of the trabecular and cortical compartments of femur and humerus under conditions of hyperglycemia are similar and are characterized by a reduced bone mass, bone disorder progression and remodeling disorders. Conclusions: Prolonged uncontrolled experimental hyperglycemia leads to slow growth of femur and humerus in mature rats, which is accompanied by an increase in microarchitecture disorder of the trabecular and cortical compartments, causing miniaturization of bones and, consequently, violation of their biomechanical properties and increased risk of fractures.

Sign in / Sign up

Export Citation Format

Share Document