The diversity of leaves and asiaticoside content on three accessions of Centella asiatica with the addition of chicken manure fertilizer

2020 ◽  
Vol 21 (3) ◽  
Author(s):  
Happy Maratul ◽  
YULI WIDYASTUTI ◽  
SUPRIYONO ◽  
Ahmad Yunus
Keyword(s):  
Leaf Morphology ◽  
Centella Asiatica ◽  
Leaf Size ◽  
Chicken Manure ◽  
Leaf Width ◽  
Petiole Length ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Chloroform Methanol ◽  
Observation Results

Abstract. Mumtazah HM, Supriyono, Widyastuti Y, Yunus A. 2020. The diversity of leaves and asiaticoside content on three accessions of Centella asiatica with the addition of chicken manure fertilizer. Biodiversitas 21: 1035-1040. Centella asiatica (L.) commonly known as an essential medicinal herb. This plant has high diversity, especially in leaf morphology. The main objective of this research was to determine the morphology of leaves and the effect of chicken manure dosage on the increase of asiaticoside content. The leaves of three accessions characterized, and then C. asiatica planted. The research design used a completely randomized design (CRD) factorial, with the first factor is accession (C) with 3 levels (C1: accession 1, C2: accession 2, C3: accession 3). The second factor was chicken manure with 4 levels dosage (P1: 0 ton ha-1, P2: 10 tons ha-1, P3: 15 tons ha-1 and P4: 20 tons ha-1). Asiaticoside analysis used the TLC method with chloroform: methanol: water (65:25:4) as mobile phase. The result from this research showed that the diversity in leaf morphology of three accessions is in leaf size and petiole length. Accession 3 has the widest leaf size (7.3 cm) and the longest petiole (9.8 cm), accession 2 with a leaf width of 5.8 cm and petiole length in 8.5 cm, while the accession 1 has the least leaf width (5.3 cm) and the length of the petiole is the shortest with 7.3 cm. Observation results of asiaticoside showed that the highest content of asiaticoside produced by accession 3 (0.19%), then accession 1 with a content of 0.15%, while accession 2 showed the lowest asiaticoside content (0.13%). The treatment of chicken manure addition significantly increase asiaticoside content in accessions 1 and 3, but in accession 2 it was not significantly increased. The highest content of asiaticoside (0.34%) were in accession 3 with the addition of chicken manure 20 tons ha-1 (P4).

scholarly journals UJI EFEKTIVITAS EKSTRAK ETANOL DAUN PEGAGAN (Centella asiatica (L.) Urban) SEBAGAI ANTIDIABETES TERHADAP TIKUS PUTIH JANTAN (Rattus norvegicus) YANG DIINDUKSI ALOKSAN

PHARMACON ◽  
2021 ◽  
Vol 10 (1) ◽  
pp. 736
Author(s):  
Grace Laury Tulung ◽  
Widdhi Bodhi ◽  
Jainer Pasca Siampa
Keyword(s):  
Rattus Norvegicus ◽  
Design Method ◽  
Centella Asiatica ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Flavonoid Compound ◽  
Randomized Design ◽  
Decrease Blood Glucose ◽  
Completely Randomized Design

ABSTRACT Gotu Kola Leaf (Centella asiatica (L.) Urban) are known to contain flavonoid compound, and flavonoid are known to decrease blood glucose level by stimulating pancreas beta cells to produce insulin. The aim of this research is to find out wether the ethanol extract of gotu kola leaf (Centella asiatica (L.) Urban) has the antidiabetic effectiveness or not. This research used Completely Randomized Design method. There were 15 rats used in this research that were split into 5 groups that is negative control (Aquadest), positive control (Metformin), and ethanol extract of gotu kola leaf with the dosage of 5.4 mg;10.8 mg;21.6 mg. The acquired data were analyzed with ANOVA test and LSD test. The analysis result showed that ethanol extract of gotu kola leaf had antidiabetic effectiveness against male white rat. Keywords:  Centella asiatica (L.) Urban, antidiabetic, Rattus norvegicus  ABSTRAK  Daun Pegagan (Centella asiatica (L.) Urban) diketahui mengandung zat flavonoid, dimana flavonoid telah diketahui mempunyai kemampuan menurunkan kadar glukosa darah dengan merangsang sel beta pankreas untuk memproduksi insulin. Penelitian ini bertujuan untuk mengetahui apakah ekstrak etanol daun pegagan memiliki efekivitas antidiabetes. Penelitian ini menggunakan metode Rancangan Acak Lengkap. Tikus yang digunakan sebanyak 15 ekor dan dibagi dalam 5 kelompok yaitu kontrol negatif (aquadest), kontrol positif (metformin), dan ekstrak etanol daun pegagan dengan dosis 5,4 mg; 10,8 mg; 21,6 mg. Data yang ada dianalisis dengan menggunakan uji ANOVA dan uji LSD. Hasil analisis menujukkan bahwa ekstrak etanol daun pegagan memiliki efektivitas antidiabetes terhadap tikus putih jantan (Rattus norvegicus). Kata kunci: Centella asiatica (L.) Urban, antidiabetes, Rattus norvegicus

scholarly journals The effect of organic fertilizer chicken on the growth of papaya plants in palm oil plantation: a case study of Naga Sari Village, Mestong, Muaro Jambi District

2021 ◽  
Vol 894 (1) ◽  
pp. 012030
Author(s):  
A T Maryani ◽  
N Mirna ◽  
F F Bahar
Keyword(s):  
Oil Palm ◽  
Organic Fertilizer ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Animal Waste ◽  
Chemical Fertilizers ◽  
Randomized Design ◽  
Two Factors ◽  
Completely Randomized Design

Abstract The use of chemical fertilizers in agriculture has caused soil degradation and is harmful to human health. Organic fertilizers made from animal waste have received worldwide attention because they are not detrimental to health or the environment. This paper describes the effect of using organic fertilizers from chicken manure on the growth of papaya plants. A completely randomized design was used in this study using two factors and three repetitions; the variations of the fertilizers given were 0, 50, 100, 150, and 200 g. This study informed that organic chicken manure had a significant effect on the growth of oil palm and papaya plants. The best growth is oil palm and papaya plants given 200 g of organic fertilizer from chicken manure.

scholarly journals PENGARUH WAKTU MASERASI TERHADAP AKTIVITAS ANTIOKSIDAN EKSTRAK DAUN PEGAGAN (Centella asiatica (L.) Urban)

2020 ◽  
Vol 9 (4) ◽  
pp. 482
Author(s):  
Ni Komang Trisna Rahayu ◽  
I Dewa Gede Mayun Permana ◽  
GA. Kadek Diah Puspawati
Keyword(s):  
Antioxidant Activity ◽  
Experimental Design ◽  
Leaf Extract ◽  
Centella Asiatica ◽  
Total Flavonoid ◽  
Time Duration ◽  
Time Treatment ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Effect On Yield

This research aimed to determine the effect of maceration time on the antioxidant activity of pegagan leaf extract and to determine the best maceration time with highest antioxidant activity of pegagan leaf extract. The experimental design used was Completely Randomized Design with maceration time duration there were 18, 24, 30, 36, 42, and 48 hours. All of the treatment repeated three times to obtained 18 units of experiments. The data analyzed by analyze of variance, if the treatment had a significant effect followed by The Duncan Test. The results showed that the maceration time treatment had a significant effect on yield, total phenolik, total flavonoid, total tannin and antioxidant activity of pegagan leaf extract. The 24 hour maceration time was the best treatment with antioxidant activity 66.67%, IC50 632.82 ppm, a yield of 24.30%, a total phenolik of 57.85 mgGAE / g, a total flavonoid of 105.28 mgQE / g. total tannin 54.09 mgTAE / g.

scholarly journals Respon Pertumbuhan Indigofera (Indigofera zollingeriana) yang Diberikan Pupuk Kotoran Kelinci dan Biochar

2021 ◽  
Vol 2 (1) ◽  
pp. 19-26
Author(s):  
Gresy Eva Tresia ◽  
A. Saenab
Keyword(s):  
Growth Response ◽  
Chicken Manure ◽  
Marginal Land ◽  
Stem Height ◽  
Randomized Design ◽  
Increase Productivity ◽  
Number Of Leaves ◽  
Indigofera Zollingeriana ◽  
Completely Randomized Design ◽  
Rabbit Manure

Application of organic manures for improvement soil fertility is expected to increase productivity of Indigofera zollingeriana in marginal land agro-ecosystems.The aim of this study was to evaluate the growth response of Indigofera zollingeriana on application of rabbit and biochar fertilizers. This study used a completely randomized design with 3 treatments and 10 replications. The treatments consisted of T0 (chicken manure biofertilizer as control), T1 (biochar), and T2 (biochar + rabbit manure biofertilizer). The variables observed were stem height (cm), number of stalks, and number of leaves. Application of rabbit manure biofertilizer and biochar (T2) gave the highest plant height, number of stalks and leaves Indigofera of 45.68±4.18 cm, 35.80±1.03 stalk, and 128.5±11.65 leaves, respectively. Also, application T2 enhanced growth vegetative Indigofera started at 7 weeks of age (35th days after planting). It could be concluded that the combination of biochar and rabbit manure biofertilizer gives the optimal result in the growth of Indigofera.

scholarly journals Potensi tepung daun sirsak, serai, kenikir, jeruk nipis, dan kluwih terhadap kumbang bubuk kedelai (Callosobruchus analis F.) pada kedelai (Glyxine max L.) dalam simpanan

2020 ◽  
Vol 1 (2) ◽  
pp. 55
Author(s):  
Laily Dwi Dzulhijja ◽  
Wagiyana W ◽  
Sigit Prastowo
Keyword(s):  
Seed Weight ◽  
Probit Analysis ◽  
Soybean Seeds ◽  
Seed Damage ◽  
Randomized Design ◽  
Effect Of Treatment ◽  
Completely Randomized Design ◽  
The Difference ◽  
Short Time ◽  
Observation Results

This study aims to determine the effect of soursop leaf flour, lemongrass flour, kenikir leaf flour, lime leaf flour, and kluwih leaf flour on: 1) mortality, 2) damage to seeds, 3) shrinkage of seed weight, 4) appearance of adult C. analysts on soybean seeds in deposits and 5) laying eggs of C. analis imago female eggs, 6) probit analysis LT50. The experiment was carried out using a Completely Randomized Design (CRD). As treatments are: A) controls; B) soursop leaf flour in doses of 1 g, 2 g, and 3 g; C) Serai leaf flour with doses of 1 g, 2 g, and 3 g; D) kenikir leaf flour with doses of 1 g, 2 g, and 3 g; E) lime leaf flour with doses of 1 g, 2 g, and 3 g; and F) kluwih leaf flour with doses of 1 g, 2 g, and 3 g. Each treatment uses five replications. The observed parameters were analyzed by "F" test variance then if it was significantly different to find out the difference in the effect of treatment, an Tukey HSD Test was conducted at a level of 5% distrust. The best mortality results using the treatment of soursop leaf flour dose 3 g on 5 days amounted to 1.80 tails; 1 month is 6.60; 2 months totaling 13.40; and 3 months totaling 11.20. The results of the observation of the number of eggs produced by C. analis the best analyst was the treatment of soursop leaf flour dose 3 g on 5 days amounting to 139 items, 1 month 119.20 items; 2 months 91.20 items; and 3 months 72.20 items. The best imago observation results were treatment of soursop leaf flour dose 3 g at 1 month amounting to 132.20 tails; while at 2 months there were 79 heads. The best observation of seed damage and shrinkage of seed weight was the treatment of soursop leaf flour with a dose of 3 g, in the damage of seeds of 67.40 grains of seed damage; while the seed weight is reduced by 17.94 g. The results of the best analysis of Probit LT50 were treatment of soursop leaf flour with a dose of 3 g because it could kill 50% of the population C. analis with a relatively short time were 9 days after treatment.

scholarly journals PENGARUH LEVEL BOKASHI KOTORAN AYAM TERHADAP PERTUMBUHAN VEGETATIF SORGUM BROWN MIDRIB (BMR) PADA KONDISI TERNAUNG

ZOOTEC ◽  
2017 ◽  
Vol 38 (1) ◽  
pp. 50
Author(s):  
Claudia F. Rawung ◽  
S.D Anis ◽  
Rustandi . ◽  
W.B. Kaunang
Keyword(s):  
Plant Height ◽  
Vegetative Growth ◽  
Research Result ◽  
Chicken Manure ◽  
Leaf Number ◽  
Brown Midrib ◽  
Leaf Analysis ◽  
Randomized Design ◽  
Completely Randomized Design ◽  
Better Than

THE INFLUENCE CHICKEN MANURE BOKASHI ON VEGETATIVE GROWTH OF BROWN MIDRIB SORGHUM (BMR) UNDER SHADE. The aim of this experiment was to study the effects of application of chicken manure bokashi on the vegetative growth of Brown Midrib (BMR) sorghum.  Completely Randomized Design (CRD) has been used according to Steel and Torrie (1995). The treatments were level of chicken manure  bokashi as follows B1= 0 gram; B2= 200 gram; B3= 400 gram and B4=  800 gram/polybag, with 7 replications to form 28 numbers of observations. The variables measured were plant height, leaf number and  length of leaf. Analysis of Variance showed that treatments effected significanly different (P<0.05) on plant height, leaf number and length of leaf. Result of Tuckey Simultenaus Test showed that between treatment B3 and B4 were not significant different (P>0.05) on all variable measured, but both treatments gave a good performance of all variable measured significantly (P<0,05) better than treatments  B2 and B1. Based on this research result it can be concluded that utilization of chicken manure bokashi up to 400 gram/polybag could provide nutrients requirement to support vegetative growth of sorghum BMR in term of height of plant, leaf number and length of leaf. Keywords: Bokashi, chicken manure, growth, BMR sorghum, shaded.

scholarly journals Pengaruh Pemberian Pupuk Kotoran Ayam dan Urea terhadap Pertumbuhan dan Hasil Tanaman Bawang Daun pada Tanah Gambut Pedalaman

2018 ◽  
Vol 4 (1) ◽  
pp. 29-37
Author(s):  
Fredy Agus Saputra Pantie ◽  
Titin Apung Atikah ◽  
Lusia Widiastuti
Keyword(s):  
Plant Height ◽  
Peat Soil ◽  
Fertilizer Application ◽  
Chicken Manure ◽  
Growth And Yield ◽  
Single Factor ◽  
Fresh Weight ◽  
Urea Fertilizer ◽  
Randomized Design ◽  
Completely Randomized Design

The purpose of this study was to determine the effect of chicken manure and urea on the growth and yield of onion leaves on inland peat soil. The design used was a Completely Randomized Design (CRD) factorial with two treatment factors. The first factor is that chicken manure fertilizer application consists of 4 levels, namely: 0, 5, 10 and 15 tons/ha, and the second factor is the provision of urea that consists of 3 levels, namely: 0, 125 and 250 kg/ha. The variables measured were plant height and fresh weight of the leaves of onion plants. The results showed that the interaction between chicken manure and urea fertilizer had no significant effect on all variables observed at all ages observed. Single factor giving 15 tons/ha of chicken manure fertilizer showed growth and better results on the average variable plant height and fresh weight of the leaves of onion plants in inland peat soil. Single factor giving of urea 250 kg/ha showed growth and better results on the average variable plant height and fresh weight of onion plants in inland peat soil.

scholarly journals PENGARUH PEMBERIAN HORMON NAA, PUPUK GANDASIL DAN PUPUK GROWMORE PADA PERTUMBUHAN TANAMAN ANGGREK

BUANA SAINS ◽  
2020 ◽  
Vol 19 (2) ◽  
pp. 13
Author(s):  
Astri Sumiati ◽  
Astutik Astutik
Keyword(s):  
Plant Community ◽  
Economic Value ◽  
Leaf Length ◽  
Ornamental Plant ◽  
Leaf Width ◽  
Factor I ◽  
Randomized Design ◽  
Factor Ii ◽  
Completely Randomized Design ◽  
Leaf Fertilizer

Orchid is an ornamental plant, including the Orchidaceae tribe, which is widely favored among the ornamental plant community because it has high economic value, unique shapes, attractive colors and longer-lasting blooming flowers, one of which is a Phalaenopsis orchid. One of the efforts to accelerate the vegetative phalaenopsis can be done with the application of the NAA hormone and leaf fertilizers such as Gandasil fertilizer and Growmore fertilizer. The aims of this research to determine the effect of NAA, Gandasil and Growmore fertilizer types on the growth of hybrid Phalaenopsis orchids. The study was conducted at Greenhouse Jalan Tlogomas Kec. Lowokwaru, Malang City, for 5 months. The experiment was carried out using a factorial Completely Randomized Design (CRD) consisting of 2 factors namely, the factor I: NAA concentration: control 0 ppm (N0), 100 ppm (N1), 200 ppm (N2), and 300 ppm (N3). Factor II: leaf fertilizer, Growmore (P1) and Gandasil D (P2). Variables observed included: when buds appeared, leaf length, leaf width and percentage of live plants. The results showed that there was an interaction between the concentration of NAA and the type of leaf fertilizer on the emergence of shoots. When the fastest shoots appear at 100 ppm NAA, Growmore fertilizer (N1P1), which is 29 days. The NAA hormone influences the increase in leaf length and leaf width until 16 weeks of age. The best growth of hybrid Phalaenopsis was obtained at the administration of 200 ppm NAA with an increase in leaf length of 1.49 cm and an increase in leaf width of 1.06 cm until 16 weeks of age. Growmore and Gandasil D did not affect the vegetative growth of hybrid Phalaenopsis Orchid plants.

scholarly journals PENGARUH CAMPURAN TANAH GAMBUT DAN PUPUK KANDANG AYAM TERHADAP PERTUMBUHAN Caliandra callothyrsus

2021 ◽  
Vol 9 (2) ◽  
pp. 199
Author(s):  
Adizar Razzaq Ichsani ◽  
Burhanuddin Burhanuddin ◽  
Siti Latifah
Keyword(s):  
Plant Growth ◽  
Raw Materials ◽  
Peat Soil ◽  
Chicken Manure ◽  
Research Purpose ◽  
Pellet Production ◽  
Randomized Design ◽  
Dry Wood ◽  
Completely Randomized Design

Kaliandra (Calliandra callothyrsus) is a plant that includes of  Fabaceae family. Kaliandra has  200 species in a medium-sized tree with compounded flowers. benefits of Kaliandra can produce fast and qualified raw materials energy, especially for pellet production. Producing Energy is for commercial requirements about 4600 kcal per kg of dry wood and 7200 kcal per kg of charcoal. This research purpose is to get the best media in Kaliandra growth. The method of research has been used by Completely Randomized Design (CRD). The research consisted of 6 treatments of planting media (M0, M1, M2, M3, M4, M5).  Those treatments have been 5th replicated therefore become 30 experimental units. These results showed best media is M5 (peat soil: Chicken Manure = 3:1). M5 is the best plant growth media.Keywords: Calliandra growth at peat media AbstrakKaliandra (Caliandra callothyrsus) merupakan tanaman anggota family Fabaceae. Kaliandra memiliki anggota sekitar 200 jenis wujudnya berupa pohon berukuran sedang dengan bunga tersusun majemuk. Manfaat dari Kaliandra dapat menghasilkan bahan baku energi secara cepat dan berkualitas terutama untuk  produksi pelet. Energi dihasilkan untuk syarat komersial sekitar 4600 kkal per kg kayu kering dan 7200 kkal panas per kg arang. Penelitian ini bertujuan untuk mendapatkan media terbaik dalam pertumbuhan Kaliandra. Metode yang digunakan adalah eksperimen dirancang menggunakan Rancangan Acak Lengkap (RAL). Percobaan terdiri dari 6 perlakuan media tanam (M0, M1, M2, M3, M4, M5). Penelitian ini menggunakan 5 kali ulangan sehingga terdapat 30 unit percobaan. Hasil penelitian menunjukan bahwa media terbaik adalah M5(Tanah gambut: Pupuk kandang ayam = 3:1). Media M5 merupakan media dengan pertumbuhan tanaman terbaik.Kata kunci: Pertumbuhan kaliandra pada media gambut

The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro

2012 ◽  
Vol 10 (2) ◽  
pp. 54-60
Author(s):  
IRMA PUTRI HAYANTI ◽  
RETNA BANDRIYATI AMIPUTRI ◽  
PRASWANTO PRASWANTO
Keyword(s):  
Shoot Growth ◽  
Organic Fertilizer ◽  
Centella Asiatica ◽  
Culture Method ◽  
Shoot Formation ◽  
Randomized Design ◽  
Leaf Formation ◽  
Medicinal Crop ◽  
Completely Randomized Design

Hayanti IP, Amiputri RB, Praswanto. 2012. The effect of liquid organic fertilizer and pearl grass extract on shoot growth of gotu kola (Centella asiatica) in vitro. Biofarmasi 10: 54-60. Gotu kola (Centella asiatica L.) is a medicinal crop, needed by local industry of pharmacy. Gotu kola in Indonesia is not enough to supply in market requisites. Tissue culture method was used to multiply gotu kola with a combination of medium from liquid organic fertilizer and pearl grass extract. The purposes of the research were to determine the effect of liquid organic fertilizer and pearl grass extract on the shoot growth of gotu kola in vitro, and to obtain the optimal concentration of liquid organic fertilizer and pearl grass extract combination on the shoot formation of gotu kola in vitro. The research was conducted in November 2010 to June 2011 in Plant Physiology and Biotechnology Laboratory, Faculty of Agriculture, Sebelas Maret University, Surakarta. The experimental design used was a Completely Randomized Design (CRD) with two treatment factors and three replications. The first factor was liquid organic fertilizer concentrations, i.e. 0 mL/L, 2 mL/L, 4 mL/L and 8 mL/L. The second factor was pearl grass extract, i.e. 0 mL/L, 3 mL/L, 6 mL/L and 12 mL/L. Variables observed were the percentage of shoot formation, the time of shoot formation, the number of shoot, the length of shoot, the percentage of leaf formation, the time of leaf formation and the number of leaf. The result of the research showed that not all of treatments provided shoot formation. The combination of liquid organic fertilizer concentrations 0 mL/L and pearl grass extract 12 mL/L showed the best results, in which the average of length of shoot was 2.16 cm, the number of shoot was 8 and the number of leaf was 8.

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