scholarly journals Vitamin D₃ levels in the maternal serum, cord blood, and placenta of preeclamptic pregnant women

2020 ◽  
Vol 29 (2) ◽  
pp. 149-53
Author(s):  
Noroyono Wibowo ◽  
Rima Irwinda ◽  
Yohanes Handoko
Keyword(s):  
Vitamin D ◽  
Cord Blood ◽  
Early Onset ◽  
Late Onset ◽  
Placental Tissue ◽  
Maternal Serum ◽  
Cross Sectional ◽  
Vitamin D Levels ◽  
Early Onset Preeclampsia ◽  
Tissue Vitamin

BACKGROUND Preeclampsia is affected by oxidative stress, a free-radical produced as a by-product of endothelial damage, and antioxidant imbalance, such as vitamin D₃. This study was aimed to compare the vitamin D₃ levels in the placenta, cord blood, and maternal serum between patients with and without preeclampsia.  METHODS This cross-sectional study included 86 patients from Cipto Mangunkusumo Hospital and Tangerang District Hospital, in which 47 had preeclampsia (13 early-onset and 16 late-onset preeclampsia cases) and 39 had no preeclampsia. The placenta, cord blood, and maternal serum were taken after labor, then were analyzed according to preeclampsia and non-preeclampsia; furthermore, the preeclampsia group was analyzed in a subgroup of early- and late-onset preeclampsia. This is analyzed with either unpaired t-test, Mann–Whitney U test, or Kruskal–Wallis test.  RESULTS The maternal serum, cord blood, and placental tissue vitamin D₃ levels (16.30 [6.20–49.00], 11.80 [3.50–38.60], and 49.00 [22.00–411.00] ng/ml, respectively) of the preeclampsia group were similar to those of the non-preeclampsia group (13.50 [4.80– 29.20], 11.70 [1.00–28.80], and 43.40 [11.80–153.00] ng/ml, respectively) (p = 0.459, 0.964, and 0.354, respectively). However, the placental tissue vitamin D₃ levels in early-onset preeclampsia (79.00 [36.00–411.00] ng/ml) were higher than those in late-onset preeclampsia (40.00 [22.00–171.00] ng/ml) (p = 0.006).  CONCLUSIONS The vitamin D₃ levels between patients with and without preeclampsia were similar. However, the placental tissue vitamin D₃ levels in early-onset preeclampsia were higher than those in late-onset preeclampsia, possibly because of the different pathophysiology between early- and late-onset preeclampsia. 

scholarly journals The Difference in Maternal Serum Hypoxia-Inducible Factors-1α Levels between Early Onset and Late-Onset Preeclampsia

2019 ◽  
Vol 7 (13) ◽  
pp. 2133-2137 ◽  
Author(s):  
Roza Sriyanti ◽  
Johanes C. Mose ◽  
Masrul Masrul ◽  
Netti Suharti
Keyword(s):  
Early Onset ◽  
Late Onset ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
Maternal Serum ◽  
Hypoxia Inducible Factors ◽  
Cross Sectional ◽  
Significant Difference ◽  
The Difference ◽  
Early Onset Preeclampsia

BACKGROUND: Preeclampsia can be divided into early (EOPE) and late (LOPE) onset preeclampsia. Preeclampsia is related to the failure of placentation. Accumulation of hypoxia-inducible factors (HIF)-1α is commonly an acute and beneficial respond to hypoxia, while chronically elevated is associated with preeclampsia. AIM: This study aims to evaluate the serum levels of HIF-1α in preeclampsia and normal pregnancy, and to compare the difference between early-onset and late-onset preeclampsia. METHODS: A cross-sectional comparative study was conducted among a total of 69 pregnant women at ≥ 20 weeks of gestation, were recruited at obstetrics and gynaecology department at Dr M. Djamil Padang Hospital, network hospitals, health centres. They were divided into three groups early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy. Preeclampsia was diagnosed using International Guidelines. Data were analysed by SPSS 24 program; data are presented as median and range or as mean ± standard deviation. One-way ANOVA test was used to determine the relationship between HIF-1α levels with the onset of preeclampsia. RESULTS: The results showed that the mean maternal serum HIF-1α levels in early-onset preeclampsia (EOPE), late-onset preeclampsia (LOPE), and normal pregnancy were 1366.96 ± 733.40 pg/ml, 916.87 ± 466.06 pg/ml, and 716.77 ± 541.08 pg/ml. Serum HIF-1α levels were higher in early-onset preeclampsia (EOPE), and late-onset preeclampsia (LOPE) compared to normal pregnancy. Among preeclampsia patients, serum HIF-1α was higher in EOPE than LOPE women. Statistical analysis revealed a significant difference in mean maternal serum HIF-1α between early-onset preeclampsia, late-onset preeclampsia, and normal pregnancy (p < 0.05). CONCLUSION: This study concluded that there is a significantly different level of HIF-1α between in early-onset preeclampsia, late-onset preeclampsia and normal pregnancy. Early-onset preeclampsia is the highest levels of serum HIF-1α.

Vitamin D Levels, APOE Allele, and MRI Volumetry Assessed by NeuroQuant in Norwegian Adults with Cognitive Symptoms

2021 ◽  
Vol 79 (1) ◽  
pp. 311-321
Author(s):  
Jelena Zugic Soares ◽  
Renate Pettersen ◽  
Jūratė Šaltytė Benth ◽  
Karin Persson ◽  
Carsten Strobel ◽  
...  
Keyword(s):  
Vitamin D ◽  
Late Onset ◽  
Apoe Genotype ◽  
Serum Levels ◽  
Grey Matter Volume ◽  
Cognitive Symptoms ◽  
Cross Sectional ◽  
Brain Volumes ◽  
Vitamin D Levels ◽  
Mri Volumetry

Background: Allele ɛ4 of the apolipoprotein (APOE ∈4) gene is the strongest known genetic risk factor for late-onset sporadic Alzheimer’s disease. A possible relationship between vitamin D and APOE is not yet clear. Objective: In this exploratory, cross-sectional study, we examined the association between serum levels of 25-hydroxyvitamin D [25(OH)D] and brain volumes and the associations of both serum levels of 25(OH)D and APOE polymorphism to brain volumes in 127 persons (mean age 66 years) with cognitive symptoms. Methods: All subjects were examined with fully automated software for MRI volumetry, NeuroQuant. Results: After adjustment for relevant covariates, higher serum 25(OH)D levels were associated with greater volumes of cortical gray matter on both left (p = 0.02) and right (p = 0.04) sides. When both 25(OH)D levels and APOE genotype were used as the main covariates, no significant associations were found between vitamin D level and brain volume in any of the 11 brain regions. In adjusted models, only homozygous but not heterozygous APOE ∈4 allele carriers had significantly larger inferior lateral ventricles (p = 0.003) and smaller hippocampal volume (p = 0.035) than those without ɛ4. Homozygous APOE ∈4 carriers also had significantly higher vitamin D levels (p = 0.009) compared to persons without the APOE ∈4 allele. Conclusion: Higher vitamin D levels might have a preserving effect on cortical grey matter volume.

scholarly journals Altered Maternal Serum Matrix Metalloproteinases MMP-2, MMP-3, MMP-9, and MMP-13 in Severe Early- and Late-Onset Preeclampsia

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Marzena Laskowska
Keyword(s):  
Matrix Metalloproteinases ◽  
Pregnant Women ◽  
Immunosorbent Assay ◽  
Early Onset ◽  
Late Onset ◽  
Maternal Serum ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Control Subjects ◽  
Early Onset Preeclampsia

Objective. The aim of this study was to determine whether maternal serum matrix metalloproteinases 2, 3, 9, and 13 levels differ in early- and late-onset preeclampsia and uncomplicated pregnancies. Patients and Methods. The study was carried out in 125 pregnant women (29 with early-onset preeclampsia; 31 preeclamptic patients with late-onset preeclampsia; and 65 healthy pregnant controls). Levels of MMP-2, MMP-3, MMP-9, and MMP-13 were measured in the maternal serum using an enzyme-linked immunosorbent assay. Results. Maternal serum MMP-2 levels in both the groups of preeclamptic women were significantly higher than those in the controls. Levels of MMP-3 were significantly higher in preeclamptic patients with early-onset disease; however, the MMP-3 levels in patients with late-onset preeclampsia were similar to those observed in the control subjects. MMP-9 levels were lower whereas the levels of MMP-13 were higher in both preeclamptic groups of pregnant women than in the healthy controls, but these differences were statistically insignificant. Conclusions. One important finding of the present study was that MMP-3 appears to be involved solely in early-onset preeclampsia, but not in late-onset preeclampsia. Higher levels of MMP-2 and MMP-13 and lower levels of MMP-9 seem to be related to both early- and late-onset severe preeclampsia.

scholarly journals Different Profile of Serum Leptin between Early Onset and Late Onset Preeclampsia

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Saeedeh Salimi ◽  
Farzaneh Farajian-Mashhadi ◽  
Anoosh Naghavi ◽  
Mojgan Mokhtari ◽  
Mahnaz Shahrakipour ◽  
...  
Keyword(s):  
Early Onset ◽  
Significant Positive Correlation ◽  
Late Onset ◽  
Maternal Serum ◽  
Serum Adiponectin ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Leptin ◽  
Positive Correlation ◽  
Early Onset Preeclampsia

Aim. This study was designed to clarify the role of leptin and adiponectin in preeclampsia (PE) pathogenesis and different subtypes of preeclampsia.Method. This case control study was performed in 45 PE patients and 45 healthy controls matched for age, BMI, and ethnicity. Serum leptin and adiponectin levels were determined by enzyme linked immunosorbent assay (ELISA).Results. Maternal serum leptin and adiponectin were significantly higher in PE women than controls. Serum leptin was elevated in early onset preeclampsia (EOPE) and late onset preeclampsia (LOPE) compared to controls. Among PE patients, serum leptin was higher in EOPE than LOPE women. However, serum adiponectin was not different between EOPE and LOPE women. The serum leptin was significantly higher in severe PE than mild PE. The serum adiponectin was significantly elevated in severe PE compared to controls. Significant positive correlation was observed between leptin and adiponectin and also between leptin and BMI in controls. Moreover significant positive correlation was observed between adiponectin and BMI in PE patients and controls.Conclusion. The present study showed that serum leptin level may play a significant role as a biomarker to differentiate early and late onset PE and also its relation to BMI and severity of disease.

scholarly journals PERBANDINGAN KADAR IFN-γ DAN IL-10 PADA SERUM MATERNAL ANTARA EARLY-ONSET PREECLAMPSIA DENGAN LATE-ONSET PREECLAMPSIA

2015 ◽  
Vol 17 (2) ◽  
pp. 98
Author(s):  
Siti Nur Khalida
Keyword(s):  
Early Onset ◽  
Late Onset ◽  
Cross Sectional ◽  
Ifn Γ ◽  
Early Onset Preeclampsia

AbstrakPenelitian ini bertujuan untuk membandingkan kadar IFN-γ dan IL-10 pada serum maternal antara early-onset preeclampsia dengan late-onset preeclampsia. Penelitian ini dilakukan di RSUD dr.Mohammad Suwandhie Surabaya sejak Juni – Agustus 2015, menggunakan rancangan cross-sectional. Didapatkan 13 sampel early-onset preeclampsia, 13 sampel late-onset preeclampsia, 13 sampel hamil normal <34 minggu dan 13 sampel hamil normal ≥34 minggu, total 52 sampel serum yang kemudian dilakukan pemeriksaan IFN-γ dan IL-10 serum dengan ELISA. Berdasarkan hasil analisis statistik, didapatkan nilai median kadar IFN-γ pada early-onset preeclampsia 0 pg/ml (0-149,1 pg/ml), pada late-onset preeclampsia 0 pg/ml, pada kelompok kontrol early 0 pg/ml (0-56,6 pg/ml), dan pada kelompok kontrol late 0 pg/ml (0-92,7 pg/ml). Hal ini kemungkinan terjadi karena kadar IFN-γ pada sampel lebih rendah dari ambang batas terendah deteksi kit ELISA IFN-γ human (R&D system inc). Sementara itu, nilai median kadar IL-10 pada early-onset preeclampsia adalah 91 pg/ml (6,2-163,90 pg/ml), pada late-onset preeclampsia 12,9 pg/ml (3,5 – 110,70 pg/ml), pada kontrol early 8,9 pg/ml (0-36,5 pg/ml) dan pada kontrol late 4,8 pg/ml (0-38,8 pg/ml) Secara statistik, tidak terdapat perbedaan bermakna kadar IFN-γ antara early-onset preeclampsia dengan late-onset preeclampsia, begitu pula dengan kelompok kontrol (harga p = 0,073). Sedangkan, menurut statistik didapatkan perbedaan bermakna kadar IL-10 antara early-onset preeclampsia dengan late-onset preeclampsia, begitu pula dengan kelompok kontrol (harga p <0,0001). Kesimpulan, tidak terdapat perbedaan bermakna kadar IFN-γ baik pada kelompok early-onset preeclampsia maupun late-onset preeclampsia, sedangkan kadar IL-10 pada early-onset preeclampsia lebih tinggi dari pada late-onset preeclampsia.  Kata kunci: early-onset preeclampsia, late-onset preeclampsia, preeklampsia, IFN-γ, IL-10

scholarly journals Study of correlation between maternal and cord blood vitamin D3 levels

2019 ◽  
Vol 7 (1) ◽  
pp. 20
Author(s):  
Sunil Rai ◽  
Saurav Das ◽  
Shankar Narayan
Keyword(s):  
Vitamin D ◽  
Cord Blood ◽  
Blood Sample ◽  
Vitamin D3 ◽  
Serum Vitamin ◽  
Maternal Blood ◽  
Maternal Serum ◽  
Blood Levels ◽  
Cord Blood Sample ◽  
Vitamin D Levels

Background: Vitamin D deficiency during pregnancy and in newborn period is common in this country. Vitamin D status of the mother is known to influence the vitamin D levels in the neonate, however how closely the maternal vitamin D level correlates with the cord blood Vitamin D is not clearly understood. To study the correlation between maternal and neonatal serum Vitamin D3 levels by as indicated by cord blood 25(OH)D levels and find out if there is a significant variation of cord blood 25(OH)D levels in Vitamin D sufficient and insufficient mothers.Methods: Healthy pregnant women between 18-45 years of age with no known history of chronic disease or long-term medication, consenting for the study were enrolled. Maternal blood sample was collected in peripartum period, cord blood sample was obtained after delivery from the umbilical cord after clamping. Vitamin D3 levels were measured by RIA and paired maternal and cord blood levels were statistically analyzed.Results: 569 paired samples of maternal and cord blood were analyzed. The mean maternal serum 25(OH)D level was 35.63ng/ml (sd 6.18, range 9.2-39.8) as compared to 13.52ng/ml (sd 3.79, range 7.9-27) for the neonates. 457 of the mothers were found to have sufficient, 101(18%) insufficient and 11(2%) deficient Vitamin D levels as per Endocrinological Society guidelines. In comparison, 535(94%) of the neonates had deficient levels, none of the neonates had sufficient Vitamin D levels, 34(5.99%) had insufficient levels. No significant correlation was found between maternal and neonatal serum vitamin 25(OH)D levels (r=0.007, P=0.85).Conclusions: Maternal and Cord blood serum Vitamin D3 levels were found to be poorly correlated in this study.

scholarly journals Differences in levelFms-Like Tyrosine Kinase-1 (sFlt-1), soluble Endoglin (s-Eng), and Placental Growth Factor (PIGF) between Early Onset Preeclampsia and Late Onset Preeclampsia

2018 ◽  
Vol 3 (2) ◽  
pp. 11
Author(s):  
Lita Nafratilova ◽  
Yusrawati Yusrawati ◽  
Irza Wahi
Keyword(s):  
Early Onset ◽  
Late Onset ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cross Sectional ◽  
Intrinsic Factors ◽  
Significant Difference ◽  
The Difference ◽  
Cross Sectional Design ◽  
Early Onset Preeclampsia

Early Onset Preeclampsia (EO-PE) is preeclampsia that develops before 34 weeks 'gestation, caused by intrinsic factors, while Late Onset Preeclampsia (LO-PE) is preeclampsia that develops after 34 weeks' gestation due to extrinsic and maternal factors. There is an increased production of antiangiogenic factors (sFlt-1, s-Eng and PIGF) contribute to pathophysiology of preeclampsia.This study aims to measure the difference of sFlt-1, sEng, PIGF levels between EO-PE and LO-PE. This was an observational study with cross sectional design conducted at Dr. M. Djamil, TK Hospital. III dr. Reksodiwiryo and Biomedical Laboratory FK Unand Padang from August 2017 to August 2018. The sample of this study were 26 severe preeclampsia women : 13 (EO-PE)  and 13 (LO-PE), selected using consecutive sampling. Levels of sFlt-1, sEng, PIGF were examined using the enzyme-linked immunosorbent assay (ELISA) method. Statistical analysis was performed using unpaired t test and Mann-Whitney Test. Results shown that serum levels of sFlt-1 and sEng in (EO-PE)  were 9.51 ± 0.71 ng / L, 1.44 ± 0.06 ng / mL, 5.79 ± 0.42 ng / mL while in PEAL it was 8, 89 ± 0.78 ng / mL, 1.35 ± 0.14 ng / mL, 6.72 ± 0.76. There were a significant difference with a value of p <0.05. The conclusion of this study is that the levels of sFlt-1 and sEng are higher in (EO-PE)  than(LO-PE)and PIGF levels was lower in (EO-PE) compared to (LO-PE)

scholarly journals Placental Growth Factor Level is Lower in Early-Onset Preeclampsia, while Tumor Necrosis Factor Alpha Level does not Show any Difference between Early and Late Onset Preeclampsia

2016 ◽  
Author(s):  
Christofani Ekapatria
Keyword(s):  
Serum Level ◽  
Early Onset ◽  
Late Onset ◽  
Necrosis Factor Alpha ◽  
Cross Sectional ◽  
District Hospitals ◽  
Tnf Α ◽  
Kolmogorov Smirnov ◽  
The Difference ◽  
Early Onset Preeclampsia

Objective: To analyze the difference of PlGF and TNF-α serum level between early-onset and late-onset preeclampsia. Method: This is a cross-sectional analytic comparative study comparing serum level of PlGF and TNF-α between groups with earlyand late-onset preeclampsia. Each group consists of 32 subjects who met inclusion criteria and presented to Dr. Hasan Sadikin Hospital or its district hospitals in September - November 2012. Statistical analysis was performed with Kolmogorov Smirnov test, Saphiro-Wilk test, and non-parametric Mann-Whitney test. Result: Mean of PlGF serum level in the group with early-onset preeclampsia is 53.0344±38.07140 pg/ml, while mean of which in the group with late-onset preeclampsia is 241.8063±192.8373 pg/ml (p

scholarly journals Comparison of serum soluble edoglin (sEng) level in eary onset preeclampsia, late onset preeclampsia and normal pregnant woman

2018 ◽  
Vol 25 (1) ◽  
pp. 10 ◽  
Author(s):  
Aldika Akbar ◽  
Mita Herdiyantini ◽  
Aditiawarman Aditiawarman
Keyword(s):  
Early Onset ◽  
Late Onset ◽  
Serum Levels ◽  
Normal Pregnancy ◽  
University Hospital ◽  
Maternal Circulation ◽  
Cross Sectional ◽  
Venous Puncture ◽  
Soluble Endoglin ◽  
Early Onset Preeclampsia

Objectives: This study aimed to compare the serum levels of soluble Endoglin (s-Eng) between early onset preeclampsia, late onset preeclampsia and normal pregnant women.Materials and Methods: This was an analytic observational study (Cross-Sectional) performed on 39 pregnant women with early-onset preeclampsia (EO-PE), late-onset preeclampsia (LO-PE), and normal pregnancy. The patients were consecutively chosen in Dr. Soetomo Hospital, Airlangga University Hospital and Dr. M. Soewandhi Hospital Surabaya in May-July 2016. The serum concentration of soluble Endoglin were collected by venous puncture taken from maternal circulation and measured by ELISA.Results: From this study, serum concentrations of soluble Endoglin was higher significantly on the early onset  preeclampsia compared with late onset preeclampsia and normal pregnancy (47,65 ± 40,17 vs 13,46 ± 9,48 vs 6,11 ± 1,45 ng/mL; p=0.000). Conclusion: This study shows angiogenic imbalance was more prominent compared in early-onset than late-onset preeclampsia. This may be because the placental dysfunction, placental ischemia, which produce excessive anti angiogenic factors, whic later causing endothelial dysfunction was more related to early onset preeclampsia.

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